Cosmetic composition comprising brewers' yeast extract and dihydric alcohol

Abstract

The present invention relates to a cosmetic composition comprising a brewers' yeast extract and a dihydric alcohol. The cosmetic composition comprises the brewers' yeast extract and the dihydric alcohol, and selectively promotes the growth of beneficial skin bacteria to maintain the balance of skin microorganisms, thereby being able to help skin health, such as improving damaged skin. Therefore, the cosmetic composition can be used for maintaining the microbiome balance of the skin, and can improve or increase the ultraviolet blocking effect of a sunblock, thereby being able to be used for ultraviolet blocking boosting.

Description

Cosmetic composition comprising brewer's yeast extract and dihydric alcohol

[0001] The present invention relates to a cosmetic composition for maintaining skin microbiome balance and a UV-blocking boosting cosmetic composition comprising brewer's yeast extract and dihydric alcohol.

[0002] Microbiome is a compound word of microbiota and genome, and it can be defined as a microbial community that includes microorganisms that live or coexist in all environments, including humans, animals and plants, soil, oceans, lakes, rocks, and the atmosphere, as well as their entire genetic information. Recently, it has also been used to refer to microbial communities in various environments. In addition, research is being conducted on various microbiomes, including plant microbiome, animal microbiome, environmental microbiome, human microbiome, gut microbiome, and skin microbiome, and among these, research on the human microbiome is the most extensive.

[0003] The human body is a complex, vibrant ecosystem, teeming with life. A diverse range of microorganisms reside in the stomach, skin, and other organs. Within these organs, 500 to 1,000 different species of microorganisms coexist, forming a microbial flora.

[0004] Microorganisms residing on the skin surface influence physiological functions such as human growth, nutrition, immunity, and resistance to pathogens. They also play a role in blocking the invasion of external microorganisms, especially pathogenic ones. However, because microorganisms present on the skin include both beneficial and harmful bacteria, maintaining skin microbiome balance is crucial for healthy skin, promoting the growth of beneficial bacteria while suppressing the growth of harmful bacteria.

[0005] Meanwhile, ultraviolet rays (UV) irradiated from sunlight are the main cause of skin erythema, edema, freckles, and skin cancer. Generally, UV rays are classified into ultraviolet A (UV-A, 320 ~ 400 nm), ultraviolet B (UV-B, 280 ~ 320 nm), and ultraviolet C (UV-C, 200 ~ 280 nm) depending on their wavelength. UV-C passes through the ozone layer and disappears without reaching the Earth's surface, but UV-B penetrates the epidermis and causes blemishes, freckles, and erythema, and UV-A penetrates the dermis and is known to cause skin aging and skin irritation, such as promoting skin cancer, wrinkles, and melanin formation.

[0006] The easiest way to protect your skin from UV rays is to use cosmetics containing sunscreen. Sunscreen cosmetics contain inorganic sunscreens, such as titanium dioxide and zinc oxide, and organic sunscreens, such as ethylhexyl methoxycinnamate, ethylhexyl salicylate, octocrylene, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyl triazine, and diethylaminohydroxybenzoylhexyl benzoate. They are often used in combination to complement each other's strengths and weaknesses. While increasing the sunscreen content in sunscreen cosmetics can enhance their UV protection, it can also cause skin irritation, allergies, and inflammation, such as acne, limiting the amount of sunscreen that can be added.

[0007] [Prior Art Literature]

[0008] [Patent Document]

[0009] Republic of Korea Publication Patent No. 10-2022-0004328

[0010] Republic of Korea Publication Patent No. 10-2019-0053216

[0011] Republic of Korea Publication Patent No. 10-2023-0106251

[0012] The purpose of the present invention is to provide a cosmetic composition that can maintain the skin microbiome balance by increasing beneficial skin bacteria while reducing harmful skin bacteria.

[0013] In addition, the present invention aims to provide a UV-blocking boosting cosmetic composition capable of enhancing the UV-blocking effect of a UV-blocking agent.

[0014] One aspect of the present invention provides a cosmetic composition for maintaining skin microbiome balance, comprising a brewer's yeast extract and a dihydric alcohol.

[0015] The “beer yeast” used in the present invention refers to yeast separated from beer that has completed fermentation during the beer manufacturing process. Types of the beer yeast include, but are not limited to, Saccharomyces cerevisiae, Saccharomyces pastorianus, and Saccharomyces eubayanus.

[0016] For example, the brewer's yeast may be Saccharomyces cerevisiae.

[0017] The above Saccharomyces cerevisiae is a wild yeast that has been used since ancient times to make beer, wine, bread, etc., and is believed to have been isolated from grape skins.

[0018] The “brewer’s yeast extract” used in the present invention includes an extract (dissolution) obtained by extraction treatment of brewer’s yeast, a diluted or concentrated extract, a dried product obtained by drying the extract, a conditioned or purified product of the extract, or a mixture thereof, and includes the extract itself and all formulations that can be formed using the extract.

[0019] According to one specific example of the present invention, the brewer's yeast extract may be obtained from Saccharomyces cerevisiae using an extraction method known in the art without limitation.

[0020] Specifically, the brewer's yeast extract may be extracted from Saccharomyces cerevisiae by one or more methods selected from the group consisting of crushing extraction, cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, pressure extraction, and solvent extraction. Two or more extraction methods may be combined to effectively extract the active ingredients of brewer's yeast.

[0021] The above-mentioned disruption extraction is an extraction method that destroys cell membranes through physical or chemical methods. Physical methods of the disruption extraction include mechanical disruption using a blender, mixer, homogenizer, etc., and chemical methods include disruption using enzymes, surfactants, etc., but are not limited thereto.

[0022] The above solvent extraction may be performed with one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, hexane, and mixtures thereof.

[0023] According to one specific example of the present invention, the brewer's yeast extract may be additionally subjected to a concentration process such as heat concentration, freeze concentration, vacuum low-temperature concentration, evaporation concentration, reduced pressure concentration, atmospheric pressure concentration, centrifugal concentration, etc. and / or a drying process such as hot air drying, freeze drying, spray drying, etc.

[0024] In addition, the brewer's yeast extract includes not only extracts obtained by the above-described extraction method, but also extracts obtained through conventional purification processes. For example, fractions obtained through various additional purification methods, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatographies (designed for separation by size, charge, hydrophobicity, or affinity), may also be included in the extract of the present invention.

[0025] The “dihydric alcohol” used in the present invention refers to a glycol represented by the general formula R(OH)2, and includes ethylene glycol, propylene glycol, etc. depending on the number of carbon atoms.

[0026] According to one specific example of the present invention, the dihydric alcohol may be at least one selected from the group consisting of ethylene glycol, methylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, heptamethylene glycol, and octylene glycol.

[0027] The above dihydric alcohols are widely used industrially, and in particular, butylene glycol and pentylene glycol are also used as cosmetic ingredients for the purposes of improving emulsion stability, formulation stability, and user experience.

[0028] “Microbiome” as used in the present invention refers to a community of various microorganisms such as bacteria, fungi, and viruses living in the human body, and “skin microbiome” typically refers to microorganisms that inhabit or exist on human skin.

[0029] “Maintaining skin microbiome balance” as used in the present invention means appropriately maintaining the balance of the skin microbial community by increasing beneficial skin bacteria while suppressing the growth and / or activity of harmful skin bacteria.

[0030] According to one specific example of the present invention, maintaining the microbiome balance of the skin may be achieved by increasing beneficial skin bacteria and reducing harmful skin bacteria.

[0031] Here, “increasing beneficial skin bacteria” means promoting the growth of beneficial bacteria that are present on the skin or can be introduced from the outside and activating the growth of beneficial bacteria, and “reducing harmful skin bacteria” means maintaining the balance of skin microorganisms by suppressing the growth of harmful bacteria that are present on the skin or can be introduced from the outside or inactivating the growth of harmful bacteria.

[0032] According to one specific example of the present invention, the skin beneficial bacteria and harmful bacteria include, but are not limited to, bacteria and fungi that inhabit the skin known in the art.

[0033] Specifically, the beneficial bacteria may be at least one selected from the group consisting of Staphylococcus epidermidis, Streptococcus pneumoniae, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Bifidobacterium bifidum, and the harmful bacteria may be at least one selected from the group consisting of Staphylococcus aureus and Cutibacterium acnes.

[0034] As an example of the above beneficial bacteria, Staphylococcus epidermidis is a gram-positive bacterium that is generally present on the skin and mucous membranes of humans and livestock. It produces glycerin to maintain the moisturizing effect of the skin, synthesizes organic acids to keep the skin acidic, and produces antibiotics to suppress the growth of harmful skin bacteria such as Staphylococcus aureus.

[0035] As an example of the above harmful bacteria, Staphylococcus aureus is a gram-positive facultative anaerobic bacterium that exists on the skin and nasal surfaces of healthy humans and livestock, and is known as a causative agent of not only food poisoning but also purulent diseases such as skin abscesses, otitis media, and cystitis. Cutibacterium acnes is a type of actinomycete that is anaerobic or oxygen-tolerant, and is mainly present on human skin, conjunctiva, external ear, pharynx, and female urinary tract, and induces an inflammatory response by decomposing fatty acids secreted by the sebaceous glands of hair follicles, which can cause acne or act as an opportunistic pathogen.

[0036] The cosmetic composition for maintaining the skin microbiome balance according to the present invention can maintain the skin microbiome balance by selectively increasing beneficial bacteria and reducing harmful bacteria on the skin due to the synergistic effect of mixing brewer's yeast extract and dihydric alcohol.

[0037] According to one specific example of the present invention, the cosmetic composition for maintaining the microbiome balance of the skin may include 0.01 to 5 wt% of brewer's yeast extract and 0.1 to 40 wt% of dihydric alcohol.

[0038] Specifically, the cosmetic composition for maintaining the skin's microbiome balance may contain 0.01 to 5 wt%, 0.01 to 1 wt%, 0.05 to 5 wt%, or 0.05 to 1 wt% of brewer's yeast extract based on the total weight.

[0039] In addition, the cosmetic composition for maintaining the skin microbiome balance may contain 0.1 to 40 wt%, 0.1 to 30 wt%, 0.1 to 20 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.5 to 40 wt%, 0.5 to 30 wt%, 0.5 to 20 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 1 to 40 wt%, 1 to 30 wt%, 1 to 20 wt%, 1 to 10 wt%, or 1 to 5 wt% of a dihydric alcohol based on the total weight.

[0040] In the cosmetic composition for maintaining skin microbiome balance according to the present invention, if the brewer's yeast extract is contained in an amount of less than 0.01 wt% and the dihydric alcohol is contained in an amount of less than 0.1 wt%, the growth of beneficial bacteria cannot be promoted, and thus the effect of maintaining skin microbiome balance cannot be exhibited, and ultimately the effect of improving skin condition cannot be exhibited. In addition, if the brewer's yeast extract is contained in an amount of more than 5 wt% and the dihydric alcohol is contained in an amount of more than 40 wt%, there is a problem in that the skin microbiome balance is broken because the growth or activity of both harmful bacteria and beneficial bacteria is suppressed.

[0041]

[0042] Another aspect of the present invention provides a UV-blocking boosting cosmetic composition comprising a brewer's yeast extract and a dihydric alcohol.

[0043] The “beer yeast extract” and “dihydric alcohol” used in the present invention are as described above.

[0044] “UV protection” as used in the present invention means the function of protecting the skin from ultraviolet rays of the sun through physical and / or chemical action, and ultraviolet blockers are classified into organic ultraviolet blockers that chemically absorb ultraviolet rays and inorganic ultraviolet blockers that physically scatter ultraviolet rays, depending on the ingredients.

[0045] “UV protection boosting” as used in the present invention means enhancing the UV protection effect by helping the skin absorb the UV protection agent or helping the UV protection agent settle into the skin.

[0046] The UV-blocking boosting cosmetic composition according to the present invention can improve or enhance the UV-blocking effect of a UV-blocking agent or a UV-blocking cosmetic composition containing the same due to the synergy effect of mixing a brewer's yeast extract and a dihydric alcohol.

[0047] Specifically, the above UV-blocking boosting cosmetic composition may increase the sun protection factor (SPF), which is an index indicating the effect of blocking UV-B, by 2 to 30, or 3 to 20.

[0048] According to one specific example of the present invention, the UV-blocking boosting cosmetic composition may include 0.01 to 5 wt% of brewer's yeast extract and 0.1 to 40 wt% of dihydric alcohol.

[0049] Specifically, the UV-blocking boosting cosmetic composition may contain 0.01 to 5 wt%, 0.01 to 1 wt%, 0.05 to 5 wt%, or 0.05 to 1 wt% of brewer's yeast extract based on the total weight.

[0050] In addition, the above UV-blocking boosting cosmetic composition may contain 0.1 to 40 wt%, 0.1 to 30 wt%, 0.1 to 20 wt%, 0.1 to 10 wt%, 0.1 to 5 wt%, 0.5 to 40 wt%, 0.5 to 30 wt%, 0.5 to 20 wt%, 0.5 to 10 wt%, 0.5 to 5 wt%, 1 to 40 wt%, 1 to 30 wt%, 1 to 20 wt%, 1 to 10 wt%, or 1 to 5 wt% of a dihydric alcohol based on the total weight.

[0051] In the UV-blocking boosting cosmetic composition according to the present invention, if the brewer's yeast extract is included in an amount of less than 0.01 wt% and the dihydric alcohol is included in an amount of less than 0.1 wt%, the effect of improving the UV-blocking function of the sunscreen cannot be exhibited, and if the brewer's yeast extract is included in an amount of more than 5 wt% and the dihydric alcohol is included in an amount of more than 40 wt%, skin irritation, allergies, acne, etc. may be induced.

[0052] The cosmetic composition for maintaining skin microbiome balance and boosting UV protection according to the present invention can be prepared in a liquid or solid form using bases, adjuvants, and additives commonly used in the cosmetic field. Liquid or solid cosmetics may include, but are not limited to, toners, creams, lotions, and bath products. Bases, adjuvants, and additives commonly used in the cosmetic field are not particularly limited, and include, for example, water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, and liquid paraffin.

[0053] The cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions, and may include conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers.

[0054] In the present invention, the cosmetic composition can be manufactured into any formulation commonly manufactured in the art, and can be formulated into, for example, a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a microcapsule, a microgranule, an ionic vesicle dispersant, a non-ionic vesicle dispersant, a powder foundation, an emulsion foundation, a wax foundation, a stick, a mask pack, and a spray, but is not limited thereto. More specifically, the cosmetic composition can be manufactured into the formulation of a flexible toner, a nourishing toner, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

[0055] In the present invention, when the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.

[0056] In the present invention, when the cosmetic composition is in the form of a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether may be additionally included.

[0057] In the present invention, when the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizer, or an emulsifier may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0058] In the present invention, when the formulation of the cosmetic composition is a suspension, a liquid diluent such as water or ethanol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tragacanth may be used as a carrier component.

[0059] In the present invention, when the formulation of the cosmetic composition is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.

[0060] The cosmetic composition of the present invention may be applied alone or in combination, or may be applied in combination with other cosmetic compositions other than those of the present invention. Furthermore, the cosmetic composition of the present invention may be used according to conventional methods, and the frequency of application may vary depending on the user's skin condition or preference.

[0061] In the present invention, if the cosmetic composition is soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it can be applied to the skin and then wiped off, removed, or washed off with water. For example, soaps include liquid soap, powdered soap, solid soap, and oil soap, surfactant-containing cleansing formulations include cleansing foam, cleansing water, cleansing towels, and cleansing packs, and surfactant-free cleansing formulations include cleansing cream, cleansing lotion, cleansing water, and cleansing gel, but are not limited thereto.

[0062] The cosmetic composition according to the present invention can be used to maintain the balance of skin microorganisms and thereby improve damaged skin, thereby helping skin health by selectively promoting the growth of skin beneficial bacteria by including a brewer's yeast extract and a dihydric alcohol, and can be used to improve or increase the UV blocking effect of a sunscreen, thereby improving or increasing UV blocking effect.

[0063] Figure 1 shows the effect of brewer's yeast live bacteria and brewer's yeast extract according to one embodiment of the present invention on the growth of skin beneficial bacteria.

[0064] Figure 2 shows the effect of brewer's yeast extract according to one embodiment of the present invention on skin cells.

[0065] Figure 3 shows the effect of brewer's yeast extract according to one embodiment of the present invention on the biofilm formation ability of skin beneficial bacteria and harmful bacteria.

[0066] Figure 4 shows the effect of brewer's yeast extract according to one embodiment of the present invention on the growth of beneficial and harmful skin bacteria.

[0067] Figure 5 shows the boosting effect of brewer's yeast extract and butylene glycol on an inorganic UV blocker according to one embodiment of the present invention.

[0068] Figure 6 shows the boosting effect of brewer's yeast extract and butylene glycol on an organic UV blocker according to one embodiment of the present invention.

[0069] Figure 7 shows the boosting effect of brewer's yeast extract and pentylene glycol on an inorganic UV blocker according to one embodiment of the present invention.

[0070] Figure 8 shows the boosting effect of brewer's yeast extract and pentylene glycol on an organic UV blocker according to one embodiment of the present invention.

[0071] The present invention will be described in more detail below. However, this description is provided merely as an example to aid understanding of the present invention, and the scope of the present invention is not limited by this exemplary description.

[0072]

[0073] Preparation Example 1. Brewer's yeast culture

[0074] Saccharomyces cerevisiae (ATCC 2341) was used as live yeast. S. cerevisiae was cultured with shaking at 30°C for 24 hours using YPD (yeast extract peptone dextrose) liquid medium, and the supernatant was removed by centrifugation, and only the sedimented S. cerevisiae was collected.

[0075]

[0076] Preparation Example 2. Brewer's yeast extract

[0077] Yeast lysate was prepared using the live yeast strain S. cerevisiae of Preparation Example 1. Briefly, S. cerevisiae was disrupted using lysozyme (Sigma-aldrich, 10837059001), and the alcohol generated during the live yeast culture in Preparation Example 1 was removed using an evaporator. The resulting solution was sterilized at 135°C for 6 seconds and dried using spray drying to prepare a brewer's yeast extract.

[0078]

[0079] Preparation Example 3. Dihydric alcohol

[0080] Butylene glycol and pentylene glycol were purchased from Sigma-Aldrich and used as dihydric alcohols.

[0081]

[0082] Preparation Example 4. Beneficial and harmful skin bacteria

[0083] Staphylococcus epidermidis was used as a beneficial skin bacteria, and Staphylococcus aureus and Cutibacterium acnes were used as harmful skin bacteria.

[0084] S. epidermidis (ATCC 12228) and S. aureus (ATCC 6538) were cultured in tryptic soy broth (TSB) medium at 32°C for 18 hours, then diluted with 0.85% NaCl to adjust the absorbance (600 nm) to 0.5 to 0.6.

[0085] C. acnes (ATCC 6919) was cultured in reinforced clostridial medium (RCM) liquid medium at 37°C under anaerobic conditions for 4 to 6 days, then diluted with 0.85% NaCl to adjust the absorbance (600 nm) to 0.5 to 0.6.

[0086]

[0087] Example 1. Effects of live brewer's yeast and brewer's yeast extract on the growth of beneficial skin bacteria.

[0088] The effects of brewer's yeast and brewer's yeast extract on the growth of beneficial skin bacteria were evaluated.

[0089] After preparing a 10% TSB medium containing 1% (v / v) of the brewer's yeast live culture of Preparation Example 1 or the brewer's yeast extract of Preparation Example 2, S. epidermidis was added to the medium at a concentration of > 10 6 1% (v / v) inoculation was performed to achieve a concentration of CFU / g. After incubation at 32°C for 4 hours, 10 was added using 0.85% NaCl. -1 ~ 10 -8 The culture was diluted and spread on TSA solid medium, and then cultured again at 32℃ for 24 hours to confirm the colony of S. epidermidis.

[0090] As a result, as shown in Fig. 1, the brewer's yeast extract did not affect the growth of S. epidermidis, whereas the brewer's yeast extract promoted the growth of S. epidermidis.

[0091]

[0092] Example 2. Effect of brewer's yeast extract on skin cells

[0093] To evaluate the effects of brewer's yeast extract on skin cells, a cytotoxicity test was conducted.

[0094] HaCaT cells, a keratinocyte cell line, were cultured at 37°C using DMEM medium (containing 10% FBS and 1% penicillin / streptomycin).

[0095] HaCaT cells were seeded at 1 x 10 in a 96-well plate. 4 After preparing cells / well, the brewer's yeast extract of Preparation Example 2 was treated at various concentrations (31.25, 62.5, 125, 250, 500, 1000, 2000 μg / ml). After culturing at 37°C for 48 hours, the cell viability was confirmed by measuring the absorbance at 450 nm using the Quanti-MAX™ WST-8 cell viability assay kit (Biomax).

[0096] As a control group, PBS was used instead of brewer's yeast extract.

[0097] As a result, as shown in Fig. 2, the brewer's yeast extract did not show toxicity to HaCaT cells.

[0098]

[0099] Example 3. Effect of brewer's yeast extract on the biofilm formation ability of beneficial and harmful skin bacteria.

[0100] The effect of brewer's yeast extract on the biofilm formation ability of beneficial and harmful skin bacteria was evaluated.

[0101] After preparing 10% TSB medium containing 1, 3, or 5% (v / v) of brewer's yeast extract of Preparation Example 2, 1.2 ml / well was dispensed into a 12-well plate. Then, S. epidermidis or S. aureus was added at > 10 6 1% (v / v) inoculation was performed to obtain a concentration of CFU / g. Afterwards, biofilm formation was induced on the wall of a 12-well plate by culturing at 32°C and 80 rpm for 5 days. After removing the culture medium, 1.5 ml / well of 1% crystal violet solution was dispensed so that the biofilm on the wall was submerged, and the plate was left at room temperature for 30 minutes to allow the biofilm to be stained. After removing the crystal violet solution, the plate was washed three times with sterile water. Finally, the biofilm was dissolved with 1.5 ml / well of 96% EtOH, and the biofilm formation ability was measured by measuring the absorbance (590 nm).

[0102] As a control, 10% TSB medium was used instead of brewer's yeast extract.

[0103] As a result, as shown in Fig. 3, brewer's yeast extract selectively promoted biofilm formation of S. epidermidis while inhibiting biofilm formation of S. aureus.

[0104]

[0105] Example 4. Effect of brewer's yeast extract on the growth of beneficial and harmful skin bacteria.

[0106] The effect of brewer's yeast extract on the growth of beneficial and harmful skin bacteria was evaluated.

[0107] After preparing a 10% TSB medium containing 1% (v / v) of the brewer's yeast live culture of Preparation Example 1 or the brewer's yeast extract of Preparation Example 2, S. epidermidis, S. aureus, or C. acnes was added thereto at a concentration of > 10 61% (v / v) inoculation was performed to achieve a concentration of CFU / g. After incubation at 32°C for 4 hours, 10 was added using 0.85% NaCl. -1 ~ 10 -8 The culture was diluted and spread on TSA solid medium, and then cultured again at 32℃ for 24 hours, and colonies of each strain were confirmed.

[0108] As a result, as shown in Fig. 4, the brewer's yeast extract selectively promoted only the growth of S. epidermidis, as in Example 1, without affecting the growth of S. aureus and C. acnes.

[0109]

[0110] Example 5. Effects of brewer's yeast extract and butylene glycol on the growth of beneficial and harmful skin bacteria.

[0111] The effects of brewer's yeast extract and butylene glycol on the growth of beneficial and harmful skin bacteria were evaluated.

[0112] Using 0.85% NaCl, S. epidermidis, S. aureus, or C. acnes > 10 6 After diluting to a concentration of 10 CFU / g, brewer's yeast extract (0.05% or 0.1%) and butylene glycol (1.5% or 3%) of Preparation Example 2 were added and cultured at 32°C for 4 hours. Afterwards, each strain was incubated at 10 using 0.85% NaCl. -1 ~ 10 -8 S. epidermidis and S. aureus were diluted and plated on TSA solid medium, and C. acnes was plated on RCA solid medium. After culturing again at 32℃ for 24 hours, colonies of each bacteria were confirmed.

[0113] As a control group, brewer's yeast extract (0.05% or 0.1%) of Preparation Example 2 was used.

[0114] As a result, as shown in Table 1 below, when brewer's yeast extract and butylene glycol were added, the growth of S. aureus and C. acnes was not significantly affected, whereas the growth of S. epidermidis was promoted. In particular, the higher the concentration of brewer's yeast extract or butylene glycol, the more the growth of the beneficial bacteria S. epidermidis was enhanced.

[0115] S. epidermidisS. aureusC. Acne untreated3 x 10 6 cfu / g4 x 10 6 cfu / g2 x 10 7 cfu / g Brewer's yeast extract 0.05% 2 x 10 8 cfu / g3 x 10 6 cfu / g6 x 10 7 cfu / g Brewer's yeast extract 0.1%6 x 10 8 cfu / g5 x 10 6 cfu / g5 x 10 7 cfu / g Brewer's yeast extract 0.05% + butylene glycol 1.5% 4 x 10 8 cfu / g4 x 10 6 cfu / g1 x 10 7 cfu / g Brewer's yeast extract 0.1% + butylene glycol 3% 8 x 10 8 cfu / g7 x 10 6 cfu / g2 x 10 7 cfu / g

[0116]

[0117] Example 6. UV protection boosting effect of brewer's yeast extract and butylene glycol

[0118] The UV-blocking boosting efficacy of brewer's yeast extract and butylene glycol was evaluated according to FDA standards.

[0119] After purchasing sunscreens containing inorganic UV blockers and sunscreens containing organic UV blockers on the market, samples were prepared by adding brewer's yeast extract (0.05% or 0.1%) and butylene glycol (1.5% or 3%) of Preparation Example 2 to each.

[0120] First, an untreated PMMA plate (16 cm 2 , 2-7 microns) were measured more than 5 times and set to the value before application, and then UVA was applied at 800 J / m 2 -eff (4MED) conditions. After that, the sample was weighed exactly 0.03 g and evenly applied to a PMMA plate with a finger, rotating it in all directions to reach a final mass of 0.01875 g. After leaving it in a dark room (room temperature) for more than 30 minutes, the SPF value was measured.

[0121] As a result, as shown in Fig. 5, when 0.05% and 0.1% of brewer's yeast extract were added to the sunscreen containing an inorganic UV blocker, the SPF value increased by 1.98 and 2.09, respectively, and when 1.5% and 3% of butylene glycol were added together with brewer's yeast extract, the SPF value increased by 3.18 and 4.48, respectively.

[0122] Also, as shown in Fig. 6, when 0.05% and 0.1% of brewer's yeast extract were added to the sunscreen containing an organic UV blocker, the SPF value increased by 0.55 and 1.59, respectively, and when 1.5% and 3% of butylene glycol were added together with brewer's yeast extract, the SPF value increased by 3.72 and 5.98, respectively.

[0123] These results suggest that brewer's yeast extract and butylene glycol enhance the UV protection effect of inorganic / organic sunscreens.

[0124]

[0125] Example 7. Effects of brewer's yeast extract and pentylene glycol on the growth of beneficial and harmful skin bacteria.

[0126] The effects of brewer's yeast extract and pentylene glycol on the growth of beneficial and harmful skin bacteria were evaluated.

[0127] Using 0.85% NaCl, S. epidermidis, S. aureus, or C. acnes > 10 6 After diluting to a concentration of 10 CFU / g, brewer's yeast extract (0.05% or 0.1%) and pentylene glycol (1.5% or 3%) of Preparation Example 2 were added and cultured at 32°C for 4 hours. Afterwards, each strain was incubated at 10 using 0.85% NaCl. -1 ~ 10 -8 S. epidermidis and S. aureus were diluted and plated on TSA solid medium, and C. acnes was plated on RCA solid medium. After culturing again at 32℃ for 24 hours, colonies of each bacteria were confirmed.

[0128] As a control group, brewer's yeast extract (0.05% or 0.1%) of Preparation Example 2 was used.

[0129] As a result, as shown in Table 2 below, when brewer's yeast extract and pentylene glycol were added, the growth of S. aureus and C. acnes was not significantly affected, while the growth of S. epidermidis was promoted.

[0130] S. epidermidisS. aureusC. Acne untreated3 x 10 6 cfu / g4 x 10 6 cfu / g2 x 10 7 cfu / g Brewer's yeast extract 0.05% 2 x 10 8 cfu / g3 x 10 6 cfu / g6 x 10 7 cfu / g Brewer's yeast extract 0.1%6 x 10 8 cfu / g5 x 10 6 cfu / g5 x 10 7cfu / g Brewer's yeast extract 0.05% + pentylene glycol 1.5% 1 x 10 8 cfu / g2 x 10 6 cfu / g3 x 10 7 cfu / g Brewer's yeast extract 0.1% + pentylene glycol 3% 2 x 10 8 cfu / g9 x 10 5 cfu / g8 x 10 5 cfu / g

[0131]

[0132] Example 8. UV protection boosting effect of brewer's yeast extract and pentylene glycol

[0133] The UV-blocking boosting efficacy of brewer's yeast extract and pentylene glycol was evaluated according to FDA standards.

[0134] After purchasing sunscreens containing inorganic UV blockers and sunscreens containing organic UV blockers on the market, samples were prepared by adding brewer's yeast extract (0.05% or 0.1%) and pentylene glycol (1.5% or 3%) of Preparation Example 2 to each.

[0135] First, an untreated PMMA plate (16 cm 2 , 2-7 microns) were measured more than 5 times and set to the value before application, and then UVA was applied at 800 J / m 2 -eff (4MED) conditions. After that, the sample was weighed exactly 0.03 g and evenly applied to a PMMA plate with a finger, rotating it in all directions to reach a final mass of 0.01875 g. After leaving it in a dark room (room temperature) for more than 30 minutes, the SPF value was measured.

[0136] As a result, as shown in Fig. 7, when 0.05% and 0.1% of brewer's yeast extract were added to the sunscreen containing an inorganic UV blocker, the SPF value increased by 1.98 and 2.09, respectively, and when 1.5% and 3% of pentylene glycol were added together with brewer's yeast extract, the SPF value increased by 4.13 and 9.27, respectively.

[0137] Also, as shown in Fig. 8, when 0.05% and 0.1% of brewer's yeast extract were added to the sunscreen containing an organic UV blocker, the SPF value increased by 0.55 and 1.59, respectively, and when 1.5% and 3% of pentylene glycol were added together with brewer's yeast extract, the SPF value increased by 4.92 and 14.14, respectively.

[0138] These results suggest that brewer's yeast extract and pentylene glycol enhance the UV protection effect of inorganic / organic sunscreens.

[0139]

[0140] The present invention has been described above, focusing on preferred embodiments thereof. Those skilled in the art will appreciate that the present invention can be implemented in modified forms without departing from its essential characteristics. Therefore, the disclosed embodiments should be considered illustrative rather than limiting. The scope of the present invention is set forth in the claims, not the foregoing description, and all differences within the scope equivalent thereto should be construed as being encompassed by the present invention.

Claims

1. A cosmetic composition for maintaining skin microbiome balance, comprising brewer's yeast extract and dihydric alcohol as active ingredients.

2. In claim 1, A cosmetic composition wherein the brewer's yeast extract is extracted from Saccharomyces cerevisiae by at least one method selected from the group consisting of crushing extraction, cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, pressure extraction, and solvent extraction.

3. In claim 1, A cosmetic composition wherein the above dihydric alcohol is at least one selected from the group consisting of ethylene glycol, methylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, heptamethylene glycol, and octylene glycol.

4. In claim 1, A cosmetic composition that maintains the microbiome balance of the skin by increasing beneficial skin bacteria and reducing harmful skin bacteria.

5. In claim 4, The above beneficial bacteria are at least one selected from the group consisting of Staphylococcus epidermidis, Streptococcus pneumoniae, Latobacillus acidophilus, Lacotbacillus rhamnosus, and Bifidobacterium bifidum. A cosmetic composition wherein the harmful bacteria is at least one selected from the group consisting of Staphylococcus aureus and Cutibacterium acnes.

6. In claim 1, The above cosmetic composition comprises 0.01 to 5 wt% of brewer's yeast extract and 0.1 to 40 wt% of dihydric alcohol.

7. A UV-blocking boosting cosmetic composition comprising brewer's yeast extract and dihydric alcohol as active ingredients.

8. In claim 7, A cosmetic composition wherein the brewer's yeast extract is extracted from Saccharomyces cerevisiae by at least one method selected from the group consisting of crushing extraction, cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, pressure extraction, and solvent extraction.

9. In claim 7, A cosmetic composition wherein the above dihydric alcohol is at least one selected from the group consisting of ethylene glycol, methylene glycol, propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, heptamethylene glycol, and octylene glycol.

10. In claim 7, The above cosmetic composition comprises 0.01 to 5 wt% of brewer's yeast extract and 0.1 to 40 wt% of dihydric alcohol.

Images