Magnetised physiological water solution for use in the healing treatment of skin and / or tissue wounds or injuries, in particular post-surgical surgical wounds

Abstract

The present invention relates to a magnetised physiological water solution containing 0.9% by weight of sodium chloride for use in the healing treatment of skin or tissue injuries or wounds, in particular post-surgical surgical wounds or injuries. The present invention also relates to a process for preparing the above magnetised physiological water solution and to the magnetised physiological water solution obtained by means of said process.

Description

[0001] Title: “Magnetised physiological water solution for use in the healing treatment of skin and / or tissue wounds or injuries, in particular post- surgical surgical wounds”

[0002] DESCRIPTION

[0003] Field of application

[0004] In its more general aspect, the present invention relates to the treatment of skin or tissue injuries or wounds.

[0005] In particular, the present invention relates to the use of a magnetised physiological water solution for the treatment of skin or tissue injuries or wounds, in particular but not exclusively post-surgical surgical wounds, in order to promote healing and scarring thereof.

[0006] Prior art

[0007] As it is known, a wound or injury is a disruption of the integrity of a tissue associated with a degenerative or traumatic loss of biological substance. Injuries may only affect the skin or may be deep injuries that also affect the internal muscle tissues, such as in surgical operations.

[0008] Each wound or injury undergoes a repair process that is substantially independent of the type and severity of the tissue damage. In this regard, three distinct phases are recognized in the healing process. First of all, there is an exudative or inflammatory phase that favours the detachment of the deteriorated tissue and the cleaning of the wound, then a proliferative phase that favours the development of granulation tissue followed by a differentiation and regeneration phase for the maturation and formation of the scar. The inflammatory phase is characterized by haemostasis and inflammation. In this phase, capillary vasodilation and migration of inflammatory cells (platelets, neutrophils, leukocytes, macrophages, and T lymphocytes) toward the injury site occur. In particular, neutrophil granulocytes play a central role in cleaning the injury by means of phagocytosis whereas macrophages are essential for the healing process.

[0009] The proliferation phase is characterized by epithelial formation, angiogenesis, granulation tissue formation, and collagen deposition. Angiogenesis is stimulated by multiple factors, such as TNF-a, and is essential for releasing nutrients into and around the injury site and is critical for efficient wound healing. The formation of granulation tissue is a complex event involving leukocytes, histiocytes, plasma cells, mast cells, and in particular fibroblasts, which promote tissue growth through the production of collagen.

[0010] In the final differentiation phase, the injury undergoes contraction and the granulation tissue becomes increasingly depleted of fluid and blood vessels, begins to strengthen and remodel to form scar tissue.

[0011] A drawback that sometimes occurs in the healing process of injuries or wounds, in particular in the healing of post-surgical surgical wounds or injuries, is the formation of keloid scars (keloids), which are cicatricial injuries that grow beyond the border of a skin injury. Starting from an abrasion or a wound, keloids develop a much larger and more extensive scar than the original damage.

[0012] Keloid scars (keloids) are the result of excessive growth of granulation tissue that originates at the edges of a wound and is mainly due to excessive and unregulated proliferation of fibroblasts in the deep dermis. These fibroblasts produce abnormal amounts of collagen and the overproduction of collagen, in turn, gives a solid consistency to the scar.

[0013] In general, the formation of scars depends on several factors, among which the regenerative capabilities of the specific subject. In the event of the formation of a keloid scar, an unwanted aesthetic defect is obtained, which is more or less accentuated depending on the greater or lesser extension of the scar beyond the edges of the wound or injury. In particular cases, said aesthetic defect may also lead to a functional disorder, for instance when keloid scars are formed in regions of the body, such as the neck region, the elbow and knee folds or at the level of hands or feet, to such an extent as to create functional disorders relating to movement.

[0014] To prevent the formation of keloids as much as possible during the healing process of wounds or injuries, in particular during the healing of post-surgical injuries or wounds, the use specific ointments or gel silicones applied to the site of the wound or injury in order to keep it moist, thus avoiding dehydration, is known. Moreover, the use of greasy bandages for bedsores in hospitals is well known.

[0015] However, these remedies have a reduced effectiveness and can sometimes hinder healing, making the healing process significantly longer.

[0016] AU 2015230725 discloses a magnetic dipole stabilized solution which may further comprise folic acid and / or an antibiotic for use in the treatment or improvement of a skin condition, including injuries and wounds, hypertrophic scars and keloids, wherein the treatment comprises contacting the area of skin to be treated or improved with the magnetic dipole stabilized solution.

[0017] KR202 10150656 describes a magnetized water comprising calcium acetate for use in the correction of cosmetic defects, such as wrinkles and wounds.

[0018] WO 2019 / 215165 describes a process for the preparation of magnetized water for medicinal use, characterized in that electromagnetic energies in the form of: (i) light energy with a wavelength of between 500 and 700 nm, (ii) light energy with a wavelength of between 701 and 1050 nm and (iii) an electrical energy with a maximum voltage of 240 V are applied to the water simultaneously, wherein at least one of the electromagnetic energies (i), (ii) and (iii) is applied to the water in the form of pulse trains having at least two discrete frequencies, wherein a first frequency is between 0.1 and 10 Hz and a second frequency is between 40 and 1500 Hz.

[0019] JP33 15022 describes a device for producing magnetized water comprising a tank for storing the water to be treated, a pump for pumping the water from the tank, a water circulation channel for returning the pumped water to the tank and a magnetic device arranged in the water circulation channel to generate lines of magnetic force approximately perpendicular to the flow of water.

[0020] US 2006275490 discloses a composition of physiologically ingestible matter, consisting of microstructured water consisting essentially of hydrogen and oxygen atoms and having a negative oxidation-reduction potential (ORP), which is made using water preconditioned by electrolysis, the method of preconditioning the water comprising the addition of ozone to the water; and passing the water through a magnetic flux.

[0021] WO 01 / 90004 discloses a method of producing magnetized water by means of a pulsating magnetic field, wherein the water comprises 0.5% sodium chloride (distilled water) .

[0022] JP2003251361 describes a water treatment system comprising a flow path for industrial wastewater, livestock wastewater, domestic wastewater or other water to be treated containing fine particulate matter; and a means of forming a magnetic field within the flow path, the direction of which changes as it moves in the direction of the water to be treated, by arranging a plurality of magnets along the flow path or by wrapping a coil around the flow path.

[0023] Therefore, there is a need in the field for a new product for the treatment of wounds or injuries, in particular injuries or post-surgical surgical wounds, which can promote the healing of such wounds or injuries by preventing or reducing the formation of keloids as much as possible.

[0024] Summary of the invention The Applicant has found out that a magnetised physiological water solution containing 0.9% of sodium chloride by weight on the weight of the solution is able to promote the healing of wounds or injuries, in particular post-surgical wounds or injuries, preventing or reducing the formation of keloids more effectively, thereby responding to the need highlighted above.

[0025] Therefore, according to the present invention, the above need is met by a magnetised physiological water solution for use in the healing treatment of skin or tissue injuries or wounds, in particular post-surgical surgical wounds.

[0026] Furthermore, the Applicant found out that such magnetized physiological aqueous solution can maintain its magnetic characteristics that promote the above healing effect for a relatively long duration, which can be at least 36 months.

[0027] Further features and advantages of the present invention will become clearer from the following detailed description which is provided by way of indicative and non-limiting example with reference to the appended figures.

[0028] Brief description of the figures

[0029] In these figures:

[0030] - Figures 1 and 2 show a statistical average of the results of objective aesthetic evaluations of sutured post-surgical wounds, 36 hours after surgery, each day after surgery during the hospital stay, 10 days after discharge and 30 days after surgery, in subjects who received a daily dressing of such wounds including a treatment with magnetised physiological water (Figure 1) and in subjects who received a daily dressing of such wounds including a treatment with non-magnetised physiological water (Figure 2);

[0031] - Figures 3 and 4 show a statistical average of the results of objective surgical evaluations of sutured post-surgical wounds, 36 hours after surgery, each day after surgery during the hospital stay, 10 days after discharge and 30 days after surgery, in subjects who received a daily dressing of such wounds including a treatment with magnetised physiological water (Figure 3) and in subjects who received a daily dressing of such wounds including a treatment with non-magnetised physiological water (Figure 4); and

[0032] - Figures 5 and 6 show the results of subjective surgical evaluations of sutured post-surgical wounds, carried out by medical personnel 30 days after surgery, in subjects who received a daily dressing of such wounds including a treatment with magnetised physiological water (Figure 5) and in subjects who received a daily dressing of such wounds including a treatment with non-magnetised physiological water (Figure 6) .

[0033] Detailed description

[0034] In the present description, the term “physiological water solution” or “physiological water” indicates any water solution containing 0.9% of sodium chloride and / or other physiologically acceptable salts in such a quantity as to make the solution substantially “isotonic”, namely having a salt concentration that is substantially equal to that of blood plasma. In the present invention, the physiological water is preferably sterile.

[0035] Preferably, the physiological water for use according to the present invention is a sodium chloride water solution at a concentration that is substantially equal to 0.9% by weight of the weight of the solution.

[0036] In the use according to the present invention, the physiological water is magnetised maintaining it in an open magnetic field having opposed poles. The magnetic field which the water is subjected to can be a constant or variable magnetic field (for instance to pulses), with constant or variable frequency between 120 Hz and 4200 Hz, the overall amount of electromagnetic energy calculated as the average power generated by the constant magnetic field or by the pulses lasting from 0.10 ms and 0.50 ms, is between 800 and 1500 Joules and the treatment time can be between 90 minutes and 600 minutes.

[0037] In this way, the average amount of energy transmitted to the water during the entire exposure can be in a range of 10 to 20 Joules so as to incorporate electromagnetic “information” (e.g. signals) into the water, which is beneficial in the healing treatment of wounds or injuries, in particular post-surgical wounds or injuries.

[0038] In this way, a persistence of the electromagnetic signal in the structure of the water molecules that is created by the imposed magnetic field can be achieved, in particular a persistence of at least 36 months.

[0039] The average power of the magnetic field applied in the above magnetization treatments can be between 1400 Watts and 2800 Watts, in particular 2000 Watts, and the average field density of the electromagnetic flux can be between 70 millitesla (mT) and 130 millitesla (mT).

[0040] The magnetisation process of the physiological water may be carried out with any device able to generate a magnetic field, such as for instance a magnetron or other magnetic devices known in the art.

[0041] It is known that magnetised pure water may incorporate electromagnetic information for a certain period of time, thereby assuming physicalchemical features similar to biological water, i.e. the water present in a living organism, both that inside the cell (intracellular) and that present in the extracellular matrix.

[0042] This biological water is in contact with cell membranes and biological macromolecules. It is known that close to the cell membranes, water reorganizes itself, creating a layer of a few hundred micrometers made of ordered water that is much more similar to intracellular water than to extracellular water. This layer of “informed” or interfacial water, called “exclusion zone” (EZ) by G.H. Pollack, is characterized by very pure water, with peculiar features and capable of self-purification (see references 1 and 2).

[0043] Said organization is made up of coherence domains of water dipoles that oscillate simultaneously. A necessary condition for this phenomenon to occur is the generation of a photonic electromagnetic field that is trapped in the coherence domain itself, produced by the collision of photons with a certain number of water molecules (see reference 3).

[0044] An increase in the amount of interfacial water leads to an increase in electron donors, i.e. energy for oxidation-reduction processes (see reference 4) and at the same time may be a sign of a contribution to the generation of further magnetic fields, which, if hierarchized, would represent an increase in the self-regulation potential of the living organism (see references 5 and 6).

[0045] In the use according to the present invention, the physiological water is made coherent by means of a treatment with an appropriate magnetic field that allows the water structure to reorganize with coherence domains that incorporate electromagnetic information able to interact with biological molecules and cellular membranes.

[0046] In this regard, it has been surprisingly found out that the magnetised physiological water according to the present invention promotes the healing (cicatrisation) of wounds and injuries, in particular of post- surgical wounds or injuries, thus improving the proliferation and repair of benign fibroblasts and counteracting the pathological proliferation of fibroblasts and the excessive deposition of collagen, thereby avoiding or limiting the formation of hypertrophic or keloid scars.

[0047] Without being bound to any scientific theory, it is believed that the electromagnetic information contained in the magnetised physiological water according to the present invention is able to interact with the tissues at the wound level where it is applied, thus effectively counteracting damage from free radicals and improving vascular permeability, while at the same time reducing inflammation, swelling and pain. As a result, the healing effect of the dressing on the wound surface is improved, while at the same time achieving sufficiently rapid healing of the wound or injury without substantial formation of keloid scars.

[0048] It has also been found, surprisingly, that the magnetized physiological water according to the invention can maintain the electromagnetic signals imparted with the magnetization treatment according to the invention for very long periods, equal to at least 36 months. Therefore, the magnetized water according to the invention has a relatively long shelf life during which it can maintain substantially unchanged its effectiveness for the use according to the invention in the treatment (healing) of wounds and lesions, in particular post-operative wounds or lesions. This duration of persistence of the electromagnetic signals in the physiological water according to the invention and, consequently, of its effectiveness in the use according to the invention is much higher than the duration of persistence of the electromagnetic signals of magnetized water as such or of non-physiological magnetized water with a lower sodium chloride content (for example 0.5%).

[0049] In fact, it is known from the literature (see reference 7) that the magnetization of water alters some of its chemical-physical properties, such as in particular the electrical conductivity and the pH. In particular, it has been observed that the electrical conductivity of water increases after the magnetization treatment compared to that of non-magnetized water with an increase that varies depending on different parameters, including the intensity of the applied magnetic field and the salt content of the water.

[0050] From experimentation conducted by the Applicant through conductivity measurements at variable times, it has been observed that a 0.5% sodium chloride aqueous solution, such as distilled water, when subjected to a magnetization treatment according to the invention, increases its electrical conductivity compared to that of the solution before the treatment but such conductivity returns to the value before the treatment after approximately 24 hours, thereby quickly losing the magnetic information imparted by the magnetization treatment.

[0051] Differently, a 0.9% sodium chloride physiological solution, when subjected to the same magnetization treatment, shows substantially the same increase in conductivity and such increase in conductivity is maintained for over 36 hours. Furthermore, from conductivity measurements at subsequent times during the clinical trials of the magnetized physiological aqueous solution of the invention, the substantially unaltered persistence of the electromagnetic signals and the efficacy of said magnetized solution for use according to the invention was observed for at least 12 months, the duration of such trials, and from further observations after the trials it was possible to evaluate a potential duration of persistence of the signals in the magnetized physiological solution and, consequently, of its efficacy at no less than 36 months.

[0052] It should be noted that, in the present invention, the magnetised physiological water can be used as such in the application on the wound or injury to be healed or in combination with one or more other traditional active principles used in the healing of wounds or injuries (in particular post-surgical wounds or injuries), said active principles having antiinflammatory, antimicrobial, antifungal, and / or healing properties (for instance active principles that promote healing through cellular stimulation) .

[0053] Non-limiting examples of active principles with anti-inflammatory properties include: aloe vera, arnica, devil’s claw, turmeric, calendula, mallow and combinations thereof.

[0054] Non-limiting examples of healing active principles include: hyaluronic acid, silver, titanium, dimethicone, hydrolysed collagen and combinations thereof.

[0055] A non-limiting example of an antifungal active principle is tea tree oil.

[0056] The combination of the magnetised physiological water with one or more of the above active principles may be simultaneous, sequential, or separated.

[0057] The simultaneous combination may be, for instance, in the form of a composition comprising magnetised physiological water and one or more of the above active principles wherein said composition is applied directly to the surface of the wound or injury to be healed. Said composition may be preferably in the form of a water solution or a gel.

[0058] The separated or sequential combination may provide for the application on the surface of the wound to be healed of magnetised physiological water and of one or more of the above active principles in any order, wherein said active principles may be applied separately, sequentially, or simultaneously, for instance in the form of a composition including them.

[0059] Preferably, the application of the magnetised physiological water in the form of water solution, possibly in combination with one or more of the above active principles, onto the surface of the wound to be healed is carried out by spraying or nebulizing.

[0060] After applying magnetised physiological water, possibly in combination with one or more of the above active principles, a dressing, such as gauze, may be preferably applied onto the surface of the wound.

[0061] These operations are repeated periodically, for instance daily, every 2-6 days, weekly and / or monthly after surgery until the wound or injury has completely healed.

[0062] Therefore, the present invention also relates to the use of a magnetised physiological water solution (magnetised physiological water) comprising 0.9% of sodium chloride by weight on the weight of the solution with one or more other active principles having anti-inflammatory, antimicrobial, antifungal, and / or healing properties for the healing treatment of skin or tissue injuries or wounds, in particular post-surgical surgical wounds.

[0063] In another aspect, the present invention also relates to a process for preparing a magnetised physiological water solution comprising 0.9% of sodium chloride by weight on the weight of the solution and to the magnetised physiological water solution thus obtained.

[0064] The process comprises the steps of:

[0065] - preparing a physiological water solution comprising 0.9% of sodium chloride by weight on the weight of the solution in a container,

[0066] - optionally subjecting said physiological water solution to sterilization,

[0067] - subjecting said physiological water, which has possibly been rendered sterile, to a magnetic field having frequency comprised between 120 Hz and 4200 Hz for a time comprised between 90 minutes and 600 minutes, for example 450 minutes, transmitting to the water an overall amount of energy during the entire exposure comprised between 10 and 20 Joule.

[0068] In this way, electromagnetic “information” (e.g. signals) into the water are incorporated, which is beneficial in the healing treatment of wounds or injuries, in particular post-surgical wounds or injuries.

[0069] The sterilisation of the physiological water solution may be carried out with processes that are per se conventional, for instance by boiling the solution or irradiating it with ultraviolet rays (UVC).

[0070] Preferably, the physiological water solution is a sodium chloride physiological solution prepared by dissolving common table salt (sodium chloride) in water to obtain a 0.9% solution by weight on the weight of the solution. Said solution can be brought to the boil or subjected to UVC irradiation until a sterile physiological water is obtained.

[0071] Preferably, the magnetic field is a pulsed magnetic field with variable frequency between 120 Hz and 4200 Hz, with an amount of energy for each pulse between 800 Joule and 1500 Joule, for example 1000 Joule and a duration of each pulse between 0.10 ms and 0.50 ms, for example 0.119 ms for a treatment time with the magnetic field preferably between 90 minutes and 600 minutes, for example 450 minutes. In this way, an amount of energy comprised between 10 and 20 Joule is transmitted to the water during the entire exposure to the magnetic field and electromagnetic “information” (e.g. signals) into the water are incorporated, which is beneficial in the healing treatment of wounds or injuries, in particular post-surgical wounds or injuries.

[0072] The magnetised physiological water as above obtained may be packaged in any container, made of glass or plastic, of the desired shape (for instance a bottle or flask). The container is preferably equipped with a suitable means of delivery, for instance of the spray type or a nebulizer.

[0073] The present invention will be further illustrated in the following indicative and non-limiting example.

[0074] Example

[0075] A study was conducted to evaluate the effectiveness of the magnetised physiological water in the healing process of post-surgical surgical wounds.

[0076] The magnetized physiological water used in this study is a magnetized physiological aqueous solution containing 0.9% sodium chloride by weight of the weight of the solution. It was obtained by subjecting a previously sterile physiological aqueous solution containing 0.9% sodium chloride by weight on the weight of the solution to a pulsed magnetic field with variable pulse frequency between 120 Hz and 4200 Hz, with an amount of energy for each pulse of 850 Joules and a duration of each pulse of 0. 119 ms for a treatment time of approximately 450 minutes.

[0077] As a comparison, a sterile physiological aqueous solution containing 0.9% sodium chloride by weight on the weight of the solution that was not subjected to any magnetization treatment was used.

[0078] The study was conducted on 50 female patients, who underwent surgery in the breast region, both for aesthetic and oncological reconstructive reasons with prostheses or autologous flaps and contralateral meatoplasty or mastopexy. The patients were divided into two groups of 25 and the patients in each group were randomly selected and were aged between 30 and 65 years. The patients were apparently healthy and were included in the study after standard blood tests (complete blood count, ESR, CRP, Gamma GT, protein electrophoresis, TSH, FT3, FT4, creatinine, and azotaemia) and urine tests and after written informed consent. In order to make the study as homogeneous as possible, all patients were sutured in the same way in triple layer, using absorbable suture material for the internal stitches and, for the external suture, nonabsorbable material with simple interrupted stitches. A group of patients underwent a dressing consisting of cleansing the surgical wound with amuchin solution, disinfection with chlorhexidine in water solution and application with a spray bottle, on the entire surgical wound, of a sterile magnetised physiological water solution according to the present invention. The other group of patients (comparison group) was subjected to the same dressing as above with the difference that a normal sterile physiological water solution (i.e. not magnetically treated) was applied to the entire wound instead of the magnetised sterile physiological water solution.

[0079] The dressing of the patients of the above two groups was carried out with the same methods daily at the same time, both during the period of hospitalization and at home after discharge. Each dressing was performed in the morning on an empty stomach and the patients were asked to limit their movement during the day and to carry out their work and / or usual occupations slowly, in order to create as much biological uniformity as possible, a condition which could limit the possible variations of resistance and reactance properties of the informed physiological water (magnetised) as much as possible.

[0080] The healing process was evaluated through an objective aesthetic evaluation by the patients in the study groups and through objective surgical evaluations by medical personnel carried out daily during hospitalization, weekly within the first month after discharge and subsequently every three months for one year after surgery. Each evaluation was assigned the following score: 1= insufficient, 2= sufficient and 3= optimal.

[0081] In Figures 1-6 the results of the above evaluations are shown.

[0082] In particular, Figures 1 and 2 show a statistical average of the results of the objective aesthetic evaluations of the sutured post-surgical wounds carried out daily by the patients in the two groups, 36 hours after surgery, every day after surgery during the hospitalisation period, 10 days after discharge and 30 days after surgery. It can be noted that 57% of the patients in the group that received the dressings including the application of magnetised physiological water considered the wound healing status to be optimal from an aesthetic point of view, whereas 43% of the patients in the aforementioned group considered this healing status to be sufficient from an aesthetic point of view (Figure 1). None of the patients in the above group considered that the wound healing status was insufficient or unsatisfactory from an aesthetic point of view.

[0083] Conversely, in the control group in which normal physiological water was applied to the suture site, 50% of the patients considered the wound healing status to be insufficient from an aesthetic point of view, whereas the remaining 50% of the patients considered the healing status to be only sufficient (Figure 2).

[0084] Figures 3 and 4 show a statistical average of the results of the objective surgical evaluations of the healing status of the sutured post-surgical wounds of the two groups of patients, carried out daily by the medical staff, 36 hours after surgery, every day after surgery during the hospitalization period, 10 days after discharge and 30 days after surgery.

[0085] It can be noted that in the group that received dressings including the application of magnetised physiological water, the wound healing status was considered by the medical staff to be optimal for 57% of the patients and sufficient for the remaining 43% of the patients (Figure 3). None of the patients in the above group received an insufficient or unsatisfactory evaluation on the wound healing status from a surgical point of view and, in particular, there was no evidence of excessively large and / or protruding scars from the suture site that would create a significant aesthetic effect.

[0086] Conversely, in the control group, the wound healing status was considered optimal by the medical staff only for 4% of the patients, whereas it was considered sufficient for 67% of the patients and insufficient for 29% of the patients in said group (Figure 4). In particular, in patients who received an insufficient evaluation, a significant aesthetic defect was found due to keloid scars forming on the surface of the wound.

[0087] Figures 5 and 6 show a statistical average of the results of subjective surgical evaluations of the healing status of the sutured post-surgical wounds of the two groups of patients, carried out by the medical staff 30 days after surgery.

[0088] It can be noted that in the group that received dressings including the application of magnetised physiological water, the wound healing status was subjectively considered by the medical staff as optimal for 57% of the patients, sufficient for 39% of the patients (Figure 5) and insufficient only for the remaining 4% of the patients in this group.

[0089] Conversely, in the control group, the wound healing status was subjectively considered by the medical staff as optimal only for 21% of patients, sufficient for 25% of patients and insufficient for the remaining 54% of patients in this group.

[0090] References

[0091] 1. Daniel C. Elton, Per D. Spencer, James D. Riches and Elizabeth D. Williams “Exclusion Zone Phenomena in Water - A critical review of Experimental Findings and Theories” - International Journal of Molecular Sciences, 2020, 21, 5041. 2. Abha Sharma, Colby Adams, Benjamin D. Cashdollar, Zheng Li, Nam V. Nguyen, Himasri Sai, Jiachun Shi, Cautham Velchuru, Kevin Z. Zhu and Gerald H. Pollack “Effect of Health-Promoting Agents on Exclusion- Zone Size” Dose-Response July-September 2018: 1-8.

[0092] 3. Messori C. (2019) “The Super-Coherent State of Biological Water”. Open Access Library Journal, 6: e5236.

[0093] 4. Albert Szent-Gyorgyi “Perspectives in Biology and Medicines” Johns Hopkins University Press Volume 14, Number 2, Winter 1971 pp. 239- 249.

[0094] 5. Luc Montagnier, Emilio del Giudice, Jamal Aissa, Claude Lavallee, Steven Motschwiller, Antonio Capolupo “Transduction of DNA information through water and electromagnetic waves” Electromagnetic Biology and Medicine Volume 34, Issue 2: Emilio del Giudice and the Science of Water.

[0095] 6. Emilio del Giudice, Paola Rosa Spinetti and Alberto Tedeschi “Water Dynamics at the Root of Metamorphosis in Living Organisms” ISSN 2073- 4441 www.mdpi.com / journal / water.

[0096] 7. " C. M. A. S. Pereira, L. M. G. de Oliveira et aL “Effect of Magnetic Treatment on the Electrical Conductivity of Water” Journal of Applied Physics, 2010.

Claims

CLAIMS1. Magnetised physiological water solution containing 0.9% by weight on the weight of the solution for use in the healing treatment of skin or tissue wounds or injuries.

2. Magnetised physiological water solution for use according to claim 1, wherein said wounds or injuries are post-surgical surgical wounds or injuries.

3. Magnetised physiological water solution for use according to claim 1 or 2, wherein said physiological water solution is a water solution of 0.9% sodium chloride.

4. Magnetised physiological water solution for use according to any one of the preceding claims in combination with at least another active principle having anti-inflammatory, antimicrobial, antimycotic and / or healing properties.

5. Magnetised physiological water solution for use according to claim 4, wherein said magnetised physiological water solution and said at least another active principle are applied to said wounds or injuries simultaneously, sequentially, or separately.

6. Process for the preparation of a magnetised physiological water solution containing 0.9% by weight on the weight of the solution, the process comprising the steps of:- preparing a physiological water solution containing 0.9% by weight on the weight of the solution in a container,- optionally subjecting said physiological water solution to sterilization,- subjecting said physiological water solution, which has possibly been rendered sterile, to a magnetic field having frequency comprised between 120 Hz and 4200 Hz for a time comprised between 90 minutes and 600 minutes, transmitting to the water an overall amount of energy duringthe entire exposure comprised between 10 and 20 Joule.

7. Process according to claim 6, wherein said physiological water solution, which has possibly been rendered sterile, is subjected to a pulsed magnetic field with variable frequency between 120 Hz and 4200 Hz, with an amount of energy for each pulse between 800 Joule and 1500 Joule and a duration of each pulse between 0.10 ms and 0.50 ms, for a treatment time with the magnetic field preferably between 90 minutes and 600 minutes.

8. Process according to claim 6 or 7, wherein the average power of the magnetic field applied in the above magnetization treatments is between 1400 Watts and 2800 Watts and the average field density of the electromagnetic flux is between 70 millitesla (mT) and 130 millitesla (mT).

9. Magnetised physiological water solution containing 0.9% by weight on the weight of the solution obtainable by means of a process according to anyone of claims 6 to 8.

10. Magnetised physiological water solution according to claim 9, wherein said solution is a water solution of 0.9% by weight of sodium chloride.

11. Magnetized physiological aqueous solution according to claim 9 or 10 for use in the healing treatment of skin or tissue wounds or injuries.

12. Magnetized physiological aqueous solution for use according to claim 11, wherein said wounds or injuries are post-operative surgical wounds or injuries.

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