Laboratory immunohistochemical staining slide fixing and auxiliary operation apparatus

Abstract

A laboratory immunohistochemical staining slide fixing and auxiliary operation apparatus, comprising an immunohistochemical staining slide fixing apparatus (1), a storage box (5), and a dedicated filter box for coverslipping (6). The immunohistochemical staining slide fixing apparatus (1) comprises a slide grasping spring (11), a first handle (12) and a second handle (13), one end of the first and second handles (12, 13) being fixed to two sides of the slide grasping spring (11), respectively, and the other ends each being bent inward to form first and second gripping parts (14, 15). By means of squeezing the handles (12, 13) to drive the top of the slide grasping spring (11) to arch open, multiple staining slides (2) can be inserted and positioned on the slide grasping spring (11); releasing the spring (11) can clamp and fix the slides (2) for subsequent batch immunohistochemical staining operations, such as staining and washing, significantly improving experiment efficiency. The storage box (5) enables centralized storage of multiple slide fixing apparatuses (1), preventing small fixing apparatuses (1) from being lost due to random placement, and reducing laboratory losses. The dedicated filter box for coverslipping (6) is used to place a slide (2) to be coverslipped on a filter mesh thereof for coverslipping processing, allowing residual xylene on the slide (2) to be centrally recovered, and thereby improving laboratory environmental safety and the personal safety of operators.

Description

Laboratory immunohistochemical staining section fixation and auxiliary operation device Technical Field

[0001] This invention relates to the field of experimental equipment technology, specifically to a laboratory-use device for fixing and assisting in the immunohistochemical staining of sections. Background Technology

[0002] Immunohistochemical staining is based on the fundamental principle of immunology—antigen-antibody reaction, that is, the principle of specific binding between antigens and antibodies. It uses a chemical reaction to make the chromogenic agent (fluorescein, enzyme, metal ion, isotope) labeled with antibodies appear colored to identify antigens (peptides and proteins) in tissue cells, and to study their localization, qualitative and relative quantification.

[0003] The routine steps of immunohistochemical staining typically include cleaning slides, tissue fixation, tissue embedding, sectioning, spreading, drying, dewaxing (by placing slides in xylene), dehydration (by sequentially placing slides in alcohol of different concentrations), antigen retrieval, serum blocking, addition of primary antibody, addition of secondary antibody, addition of chromogenic agent, counterstaining, dehydration, clearing, and mounting. The entire process involves several media changes and cleaning operations, making it relatively complex, especially when multiple immunohistochemical staining slides need to be processed simultaneously. Traditionally, operators manually handle slides multiple times to process, dehydrate, and clear multiple slides. This individual operation not only reduces efficiency but also increases the likelihood of operator hand contact with the staining solution and slide contamination. To improve ease of operation, multiple slides can be simultaneously fixed using the same device, allowing for simultaneous movement of multiple slides for each operation.

[0004] Chinese patent CN 217341468 U discloses a slide clamping device that is easy to disassemble, including a fixing box and clamping springs located inside the fixing box. The tension of the clamping springs is controlled by a traction rope. When the spring coils are pulled open to a distance sufficient to accommodate slides, multiple slides can be fixed between the spring coils. Releasing the pulling section causes the clamping springs to automatically spring back, thus firmly clamping the slides placed between the spring coils. While this structure can clamp multiple slides, its direct application in immunohistochemical staining experiments presents several problems: First, when using this structure to clamp multiple immunohistochemical staining slides, because the springs are pulled horizontally from both ends, the circumferential gaps between the spring coils are uniform. This results in several slides being inserted between the spring coils and arranged parallel to each other. Since multiple slides requiring batch processing are simultaneously placed into a vertical glass staining tank during the experiment, the spacing between the slides needs to be adjusted. First, the proximity of the sections means that the ends of adjacent sections furthest from the spring coil are close together. Parallel sections furthest from the spring coil are prone to sticking together during staining, leading to contact contamination, damage to the stained tissue, and affecting experimental results. Second, the device relies on the automatic spring-loaded contraction of the spring after the traction rope is released to clamp the sections. The speed and force of this automatic contraction are uncontrollable, and since the sections are thin glass sheets, the spring-loaded contraction can easily shatter the sections, affecting subsequent operations. Third, each opening and closing of the clamping spring only clamps one section at a time. First, when multiple slides need to be clamped on the clamping spring, multiple opening and resetting processes are required. This means that the slides initially clamped between the spring coils must withstand the impact force from the spring rebound multiple times, further increasing the possibility of slide breakage. Second, the process of controlling the bending of the pulling section to open the clamping spring is mainly achieved by the slide pushing the pulling section. The pulling section only contacts the front end of the slide, and there is a high possibility of misalignment during the inward pushing process, which will affect the accuracy of slide clamping. Slides that are suddenly de-stressed at the front end may also shift in position and hit the spring coil, causing slide damage. Third, after using the clamping spring to clamp the slides, the overall size of the clamping spring is small, and the space for hand operation is limited. The operability of the operator is relatively low when holding the clamping spring with multiple slides for slide cleaning, staining and other operations. Therefore, this device still has many shortcomings for immunohistochemical staining experiments. Fourth, after using the clamping spring to clamp the slides, the clamped slides and springs need to be removed from the fixing box from the device before they can be placed in the staining tank, which increases the process of loading and unloading the springs and reduces convenience.

[0005] Furthermore, before mounting slides in the laboratory, operators typically remove the slides from the mounting device. During this process, xylene from multiple slides can contaminate the workbench. Additionally, after removal, the slides are often placed directly on the workbench for mounting, leaving a residual amount of xylene. To prevent xylene spillage, operators may place paper or other absorbent materials under the slides to absorb excess xylene. However, this method does not effectively address the issue of uncontrolled xylene leakage causing workbench contamination, posing a threat to laboratory safety and the personal safety of the operators.

[0006] Therefore, it is essential to design a laboratory slide fixation device that can help with the batch processing of immunohistochemical staining slides and experimental accessories that can assist in the smooth and safe execution of the immunohistochemical staining process. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a laboratory-use immunohistochemical staining slide fixation and auxiliary operation device. This device allows for the simultaneous fixation of multiple slides for subsequent batch immunohistochemical staining operations such as immunostaining and washing. Furthermore, the slides are less prone to contamination or tissue damage during the immunohistochemical staining process, thus improving the accuracy of experimental results. In addition, this application discloses an auxiliary operation device including a storage box and a dedicated mounting filter box. The storage box is specifically designed to store the immunohistochemical staining slide fixation device proposed in this application to prevent loss. The dedicated mounting filter box filters out any xylene dripping from the slides during mounting and allows for centralized collection of the xylene, contributing to improved safety in laboratory operations.

[0008] The technical solution of the present invention is as follows: a laboratory immunohistochemical staining slide fixation and auxiliary operation device, comprising: an immunohistochemical staining slide fixation device for clamping and fixing several slides; a storage box for storing the immunohistochemical staining slide fixation device; the immunohistochemical staining slide fixation device includes a clamping spring, a first handle and a second handle, one end of the first handle and the second handle are respectively fixed to the two ends of the clamping spring, and the ends of the first handle and the second handle away from the clamping spring are respectively bent inward to form a first gripping part and a second gripping part.

[0009] Furthermore, the clip spring is composed of several spiral coils connected together, with the cross-section of the spiral coils being square and the four corners being rounded.

[0010] Furthermore, the clip spring is 4-5 cm long, the inner diameter of the clip spring is 1.5-2 cm, and the diameter of the spring wire wound to form the clip spring is 2-2.5 mm.

[0011] Furthermore, the first handle and the second handle are fixed at the two ends of the clamping spring at an angle, and the angle between the axis of the first handle and the axis of the clamping spring is 30°~35°.

[0012] Furthermore, the immunohistochemical staining section fixation device also includes a clamping and fixing component, which includes a fixing block, a first hook, a second hook, a control button, and a top-pressure spring. The fixing block is fixedly connected to the first handle, and the interior of the fixing block is hollow, forming a movable cavity. The fixing block has a hook-connecting movable port on its side near the second handle, and the hook-connecting movable port communicates with the movable cavity. The top-pressure spring is located in the movable cavity, with one end fixed to the bottom surface of the movable cavity and the other end fixedly connected to the bottom surface of the first hook. The top of the first hook has a first barb extending upward and toward the first handle at one end near the second handle, and the top surface of the first hook is fixedly connected to the bottom surface of the control button. The second hook has a second barb extending downward and toward the second handle at one end.

[0013] Furthermore, the bottom surface of the second hook is higher than the bottom surface of the first hook and lower than the top surface of the first hook, and the first barb and the second barb can be matched and hooked.

[0014] Furthermore, the immunohistochemical staining section fixation device also includes a binding and fixing component, which includes a hook strap and a fixing post; the hook strap is an annular non-elastic connecting strap, one end of which is fixed to the second handle; the fixing post includes a lower columnar part and an upper limiting disc part, one end of which is fixed to the first handle and the other end is inserted into the side of the limiting disc part; the diameter of the limiting disc part is larger than the diameter of the columnar part.

[0015] Furthermore, the storage box includes a storage box body, drawers, and handles. The storage box body has several pull-out drawers inside, and a handle is provided on the top of the storage box body. Each drawer has a handle on the outside, and a snap-fit ​​component is provided on both sides of the bottom surface inside the drawer. The first handle and the second handle can be snapped and fixed in the storage box body using the snap-fit ​​component.

[0016] Furthermore, a magnetic block is provided on the outer surface of the drawer at the end away from the handle, and a magnet is provided on the inner side of the storage box at the position corresponding to the magnetic block. The magnetic block and the magnet can be magnetically attracted to each other.

[0017] Furthermore, the laboratory immunohistochemical staining slide fixation and auxiliary operation device also includes a special mounting filter box, used to filter and collect residual liquid on the slides during slide mounting; the special mounting filter box includes a filtrate box, U-shaped grooves, and a filter screen; the filter screen includes a filter screen plate and a support rod, with two support rods fixed on opposite sides of the filter screen plate and the ends of the rods protruding from the filter screen plate; the filter screen plate has several longitudinally penetrating filter holes on both sides; the filtrate box includes a box body and a box lid, the box lid is hinged to one side of the box body and can rotate relative to the box body; the box body and the box lid are opened and closed by a magnetic switch; the box body is 2-2.5 cm high, 10-11 cm long, and 8-9 cm wide; a pair of upward-opening U-shaped grooves are respectively provided on opposite sides inside the box body, the positions of the U-shaped grooves corresponding to the positions of the support rods; after the filter screen is placed inside the box body, the support rods protruding from its edges can be placed in the corresponding U-shaped grooves.

[0018] The beneficial effects of this invention are as follows:

[0019] 1. The immunohistochemical staining slide fixing device disclosed in this application includes a clamping spring and handles fixed on both sides thereon. By squeezing the handles, the top of the clamping spring is arched and opened. Multiple staining slides can be inserted and fixed on the clamping spring in one opening and rebound process, which facilitates subsequent batch staining, cleaning and other immunohistochemical staining operations on the slides. It is no longer necessary to take the slides out one by one for corresponding processing, which can significantly improve experimental efficiency.

[0020] 2. This application uses two handles that are clamped in opposite directions to make the clamping spring open in an arched state to complete the insertion action. Since the clamping spring is bent into an arch before the insertion, even if the force on the spring is released after the insertion, the inserted and fixed slices are still radially distributed on it. When several slices connected to the fixing device are inserted into the staining tank for staining, the ends of the radially distributed slices are far apart, so the ends of adjacent slices are not easy to stick together during the staining process, which can reduce the possibility of the slices being contaminated during the experiment.

[0021] 3. The immunohistochemical staining slide fixation device disclosed in this application has a simple structure. The presence of a handle makes it easy to hold the spring holding the slide to perform the corresponding experimental operations. The operation is convenient, the slide mounting and fixation process can be completed by a single person, and the technical requirements for the operator are not high, making it easy to promote and use in the laboratory.

[0022] 4. When using the immunohistochemical staining slide fixation device disclosed in this application to clamp and fix several slides, the clamping spring only needs to undergo one opening-rebound process. This avoids the situation where the slide is repeatedly struck due to the frequent opening and rebound of the spring, reducing the occurrence of slide breakage. Furthermore, since the force of the rebound process is controllable, the impact force on the slide when the spring rebounds can be controlled to a minimum, which can further reduce the possibility of slide breakage during the fixation operation and fully improve the stability of the experimental process.

[0023] 5. This application limits and fixes the position of the handle after the spring is arched and opened to a certain angle by setting clamping or binding fasteners on the two handles of the slice fixing device. During the slide insertion process, the operator does not need to continuously apply pressure to the handle to ensure the stability of the spring, which can reduce the physical exertion of the operator. It is especially suitable for experimental personnel with less strength, and can also avoid the abnormal rebound of the spring due to accidental unloading of force, further improving the stability of the operation process.

[0024] 6. This application uses a square spring as the clamping spring. Compared with a round spring of the same specification, the square spring has a larger contact area with the slice when clamping it, which can further improve the stability of the clamping process and also further reduce the damage to the slice caused by spring rebound.

[0025] 7. This application discloses a storage box specifically for the immunohistochemical staining slide fixation device, which can realize the centralized storage of multiple slide fixation devices, avoid the loss of small-volume fixation devices due to random placement, reduce laboratory losses, and the clamp structure can limit and fix the fixation devices placed in the drawer, preventing them from moving randomly in the drawer and causing accidental damage.

[0026] 8. This application discloses a special filter box for mounting slides, which can remove the stained slides one by one from the fixing device and place them on the filter screen to await mounting. The slides to be mounted can also be placed on the filter screen for mounting. The xylene remaining on the slides can be collected and treated. At the same time, the operation of removing the slides from the fixing device before mounting can also be carried out in the special filter box. This process can minimize the contamination of the laboratory environment by xylene on multiple slides, effectively improving the safety of the laboratory environment and the personal safety of the operators. Instruction manual illustrations

[0027] Figure 1 is an isometric view of the immunohistochemical staining section fixation device disclosed in Example 1;

[0028] Figure 2 is a top view schematic diagram of the immunohistochemical staining section fixation device disclosed in Example 1;

[0029] Figure 3 is a left-side schematic diagram of the immunohistochemical staining section fixation device disclosed in Example 1;

[0030] Figure 4 is a schematic diagram of the clip spring disclosed in Embodiment 1;

[0031] Figure 5 is a diagram showing the state of the immunohistochemical staining section fixation device disclosed in Example 1 after the clamping springs are compressed and arched in opposite directions and the section is inserted between its spiral coils.

[0032] Figure 6 shows the state of the slides after they are placed in the staining tank. (a) The small figure shows the state of the slides when they are inserted into the compartments of the staining tank in the traditional operation mode; (b) The small figure shows the state of the slides after several slides are inserted into the clamping spring and the gripping force on the first / second handle is released so that the spring rebounds and clamps the slides and the slides are placed into the vertical staining tank.

[0033] Figure 7 is an isometric view of the immunohistochemical staining section fixation device with clamping fixation element disclosed in Example 2;

[0034] Figure 8 is a top view of the immunohistochemical staining section fixation device with clamping fixation element disclosed in Example 2;

[0035] Figure 9 is a diagram showing the state of the immunohistochemical staining section fixation device disclosed in Example 2 after the clamping springs are compressed and arched in opposite directions and the positions of the first handle and the second handle are limited by the clamping and fixing components.

[0036] Figure 10 is an isometric view of the clamping and fixing component disclosed in Embodiment 2;

[0037] Figure 11 is a cross-sectional schematic diagram of the clamping and fixing component disclosed in Embodiment 2;

[0038] Figure 12 is a top view of the fixation device for immunohistochemical staining sections with binding fasteners;

[0039] Figure 13 shows the state of the clamp springs of the immunohistochemical staining section fixation device after they are compressed and arched in opposite directions and the positions of the first and second handles are fixed by the binding fasteners.

[0040] Figure 14 is an isometric view of the fixed pile;

[0041] Figure 15 is an isometric view of the storage box disclosed in Embodiment 3;

[0042] Figure 16 is an isometric view of a storage box with a magnetic block-magnet assembly;

[0043] Figure 17 is an isometric view of the sealing filter box disclosed in Example 4;

[0044] Figure 18 is an isometric view of the filter screen placed in the filtrate box;

[0045] Figure 19 is a top view of the filter screen plate installed on the U-shaped groove in the filtrate box;

[0046] Figure 20 is an isometric view of a filter cartridge with a magnetic switch;

[0047] Among them, 1-immunohistochemical staining section fixation device, 2-section, 3-clamping fixation component, 4-binding fixation component, 5-storage box, 6-special filter box for mounting section;

[0048] 11-Clamping spring, 12-First handle, 13-Second handle, 14-First grip, 15-Second grip;

[0049] 21-Supporting edge;

[0050] 31-Fixing block, 32-First hook connector, 33-Second hook connector, 34-Control button, 35-Top pressure spring, 36-Moving cavity, 37-First barb, 38-Second barb;

[0051] 341 - Disc-shaped button; 342 - Control column;

[0052] 41-Hook and strap, 42-Fixing post;

[0053] 421 - Columnar portion; 422 - Limiting disc portion;

[0054] 51-Storage box body, 52-Drawer, 53-Handle, 54-Clamp, 55-Magnetic block, 56-Magnet;

[0055] 61- Filtration box, 62- U-shaped groove, 63- Filter screen, 64- Support rod, 65- Filter holes, 66- First magnetic component, 67- Second magnetic component. Detailed Implementation

[0056] The following embodiments further illustrate the content of the present invention, but should not be construed as limiting the present invention. Any modifications and substitutions made to the methods, steps, or conditions of the present invention without departing from the essence of the invention are within the scope of the present invention. Example 1

[0057] In order to enable batch staining and cleaning of immunohistochemical staining sections, this application discloses an immunohistochemical staining section fixation device, which is mainly used in the laboratory stage when performing immunohistochemical staining operations. It can be used to fix multiple immunohistochemical staining sections at the same time, so that the necessary steps of immunohistochemical staining such as staining and cleaning can be performed on multiple sections at the same time, improving the convenience of operation, increasing the efficiency of experimental operation, and reducing the possibility of section contamination due to frequent handling.

[0058] Referring to Figures 1-5, the immunohistochemical staining section fixation device includes a clamping spring 11, a first handle 12 and a second handle 13. One end of the first handle 12 and the second handle 13 are respectively fixed to the two ends of the clamping spring 11. The ends of the first handle 12 and the second handle 13 away from the clamping spring 11 are respectively bent inward to form a first gripping part 14 and a second gripping part 15.

[0059] The clamping spring 11 is composed of several spiral coils connected together. The cross-section of the spiral coil is generally square, but the four corners are rounded. The reason for choosing a specially made square spring to assemble this fixing device is that, compared with the conventional springs of the same specification (the same specification means that the inner side length of the square spring and the inner diameter of the circular spring are the same) with a circular cross-section, when the clamping spring 11 clamps the slice 2 and the slice 2 enters the spiral coil to a certain depth, the contact area between the square spring and the slice is larger than that between the circular spring and the slice. Increasing the contact area can improve the clamping stability and prevent the slice from falling out between the spiral coils during clamping or movement, which would affect subsequent experiments. At the same time, since the shape of the square spring matches the end of the slice, it can also reduce the damage to the slice caused by the spring rebound.

[0060] Preferably, the clip spring 11 is made of a corrosion-resistant alloy material to avoid corrosion and damage caused by contact with chemical reagents during the experiment.

[0061] The overall length a of the clip spring 11 is 4.5 cm, the inner diameter b of the clip spring 11 is 2 cm, and the diameter of the alloy wire wound to form the clip spring 11 is 2 mm.

[0062] To increase the arching curvature of the clamping spring 11 after bending under force, thus better opening the spiral coil for easier slice insertion, the fixing device initially fixes the first handle 12 and the second handle 13 at both ends of the clamping spring 11 in an inclined position. The angle ∠θ between the axis of the first handle 12 / second handle 13 and the axis of the clamping spring 11 is between 30° and 35°. Preferably, in this embodiment, ∠θ is set to 30°.

[0063] The specific operating steps for this fixing device are as follows:

[0064] The experimenter grasped the first and second grip portions 14 / 15 on the first and second handles 12 / 13 with two fingers of one hand, and squeezed the two handles together. At this time, the clamping spring 11 connecting the first handle 12 and the second handle 13 contracted and bent inward on the side near the handle grip portion, while the other side opened outward during the bending process, making the clamping spring 11 arched. After the outer side of the clamping spring 11 was pulled open, the distance between the outer sides of the adjacent spiral coils increased. Then, ensuring that the clamping spring 11 and the handle were on the same horizontal plane, the slice 2 could be inserted into the gap between the adjacent spiral coils. Because the spiral coils were tightly connected, after being opened for the slice to be inserted, the spiral coils themselves exerted a certain compressive force on the slice insertion end. After the slice 2 was inserted into the clamping spring 11, it was in a relatively stable state, and the slice 2 itself was not easy to fall out of the spring coils. To further improve the stability of the slice insertion process, it can also be performed on a table. The fixing device should be positioned slightly above the table surface. After inserting slice 2 between the spring coils, the fixing device can be tilted slightly so that the bottom of the free end of the slice inserted into the fixing device can be pressed against the table surface to prevent it from falling out of the spring coils. Then, based on the same operation, different slices 2 are inserted into different gaps between the spiral coils to separate the slices 2. Since the clamping spring 11 is compressed into an arch shape, after inserting slice 2, slice 2 is fixed radially on the clamping spring 11. After clamping all slices 2, loosen two fingers to reduce the gripping force on the two handle grips. The two handles slowly move in opposite directions, and the arched spring slowly retracts. During the retraction process, the inserted slice 2 can be further clamped. After the slice 2 is further clamped, even if slice 2 is facing down, it will not fall off the clamping spring 11, and the slice 2 will still be fixed radially on the clamping spring 11. Afterwards, slice 2 can be put into a vertical dyeing tank for dyeing or washing and other operations.

[0065] Since the slice 2 is clamped and fixed by the spring 11, when the slice 2 is put into the dyeing tank, it does not need to be inserted into the slots formed by the opposing baffles 21 on both sides of the dyeing tank. Before the fixing device, the operator inserts the slice 2 one by one into the slots to fix the relative position for dyeing operation. If the slice 2 is placed according to the slots (see the small figure in Figure 6(a)), the slice 2 needs to be taken out and put in one by one. It is not possible to process multiple slices 2 at the same time. The process is cumbersome and limited by the number of slots. Each dyeing tank can only dye a maximum of five slices 2 at the same time. However, after fixing the slice 2 using the fixing device disclosed in this application, since the end of the slice 2 is clamped and fixed by the clamping spring 11, it is no longer necessary to move along the groove to achieve relative fixation of the position of the slice 2. Although for the purpose of saving costs, this laboratory still chooses to use the original staining vat for staining operations, since the groove structure is abandoned during use, as shown in the small figure in Figure 6(b), the slice 2 is still inserted in a vertical state. At this time, the placement direction of the staining vat is equivalent to rotating 90° relative to the original usage state. At this time, the groove structure is located on the side, so the insertion of the slice 2 is no longer restricted by the groove. In this case, it is not only convenient to move and process the slice 2 uniformly, but also to increase the number of slices 2 processed at the same time. The number of slices put into the staining vat is no longer limited by the number of grooves. As long as the slice 2 can be put into the staining vat and the end can be in a radiating shape, the processing quantity can be increased. (It has been verified that by using the fixing device disclosed in this embodiment to fix the slices, 7 slices can be put into the same staining vat for corresponding operations at the same time).

[0066] Because the distance between the ends of adjacent sections 2 is relatively large, they are less likely to come into close contact during the staining process (see small figure in Figure 6(b)), which reduces the possibility of section 2 being contaminated. Example 2

[0067] Because the operator needs to apply relatively large force when gripping the handle and moving it in opposite directions to open the clamping spring 11, and in order to insert multiple slices one by one between the spiral coils on the clamping spring 11 after it is opened once, the clamping spring 11 needs to be kept in an arched and open state for a period of time. During this period, the fingers need to continuously apply force to the gripping part. If the fingers loosen during the insertion process due to the experimenter's lack of strength or an accident, causing the clamping spring 11 to retract, force needs to be applied again to open the clamping spring 11. This repeated opening and rebounding can easily cause the slices already inserted between the spiral coils to shift and fall out. The repeated rebounding process can also easily damage the slices. However, for experimenters with less strength, especially female operators, it is indeed quite difficult to continuously apply large force with their fingers.

[0068] To solve the above problems, this embodiment adds a clamping and fixing component 3 based on embodiment one.

[0069] Referring to Figure 7-11, the clamping and fixing member 3 is provided on the first handle 12 and the second handle 13. When the first and second handles 12 / 13 are squeezed and move closer to each other to a certain distance, the clamping and fixing member 3 can fix the two handles to limit the position of the two handles. At this time, the operator no longer needs to apply force to the handles, and the handles will not open in opposite directions. This can obviously save effort and further improve the stability of the operation process.

[0070] The clamping and fixing component 3 includes a fixing block 31, a first hook connector 32, a second hook connector 33, a control button 34, and a top pressure spring 35. One end of the fixing block 31 is fixedly connected to the first handle 12. The interior of the fixing block 31 is hollow, forming a movable cavity 36. The side of the fixing block 31 near the second handle 12 has a hook connection port that communicates with the movable cavity 36. The top pressure spring 35 is located inside the movable cavity 36, with one end fixed to the bottom surface of the movable cavity 36 and the other end fixedly connected to the bottom surface of the first hook connector 32. The top of the first hook connector 32, near the second handle 13, has an upward and downward... The first hook 37 extends in the direction of the first handle 12. The top surface of the first hook member 32 is fixedly connected to the bottom surface of the control button 34. The control button 34 extends out of the fixed block 31 through the through-hole on the top surface of the fixed block 31. One end of the second hook member 33 is fixedly connected to the second handle 13, and the other end is provided with a second hook 38 extending downward and towards the second handle 13. The bottom surface of the second hook member 33 is higher than the bottom surface of the first hook member 32 and lower than the top surface of the first hook member 32. The first hook 37 and the second hook 38 can be matched and hooked to connect the first hook member 32 and the second hook member 33 together.

[0071] The control button 34 consists of two parts: a disc-shaped button 341 and a control column 342. The disc-shaped button 341 is located on the outside of the fixed block 31. The control column 342 is coaxially fixedly connected to the bottom surface of the disc-shaped button 341. After the control column 342 moves through the through-hole on the top surface of the fixed block 31, its bottom surface is fixedly connected to the top surface of the first hook connector 32.

[0072] When two fingers of one hand press against the gripping parts of the first handle 12 and the second handle 13, causing the two handles to move closer together, the fixing block 31 and the second hook connector 33 also move closer to each other. When the second hook connector 33 enters the interior of the fixing block 31 and contacts the first hook connector 32, the first hook connector 32 will interfere with its position, thus hindering its further movement and preventing the two handles from moving closer together. At this time, the operator's other hand presses down on the disc-shaped button 341 to move the control column 342 and the first hook connector 32 downwards in sequence. At this time, the top spring 35 is compressed, and when the first hook connector 32... After the height of the first handle 12 and the second handle 13 is lower than the height of the second barb 38, the first handle 12 and the second handle 13 can be further squeezed towards each other. At this time, the second hook connector 33 can move forward further. When the disc button 341 is released, the top spring 35 returns to its original position, and the height of the first hook connector 32 rises. The first barb 37 and the second barb 38 are then hooked together in a staggered manner. In addition, when the squeezing force on the first handle 12 and the second handle 13 is released, the second hook connector 33 will return to its original position to a certain extent. The return process allows the first barb 37 and the second barb 38 to be tightly hooked together, achieving a stable matching hook connection between the first hook connector 32 and the second hook connector 33. After the first barb 37 and the second barb 38 are hooked together, the positions of the first handle 12 and the second handle 13 are fixed. Even if no further squeezing force is applied to the two handles, the clamp spring 11 can be kept in an arched and open state. This structure is particularly suitable for experimental operators with relatively little strength and will not affect the stability of the operation process.

[0073] As another embodiment, the binding fastener 4, which consists of a hook and a fixing post, can be used instead of the clamping fastener 3 to fix the position of the two handles.

[0074] Referring to Figures 12-14, the binding fastener 4 includes a hook strap 41 and a fixing post 42. The hook strap 41 is a ring-shaped non-elastic connecting strap, which can be made of non-elastic materials such as nylon. One end of the hook strap 41 is fixed to the second handle 13. The fixing post 42 is mushroom-shaped, including a columnar part 421 at the bottom and a limiting disc part 422 at the top. One end of the columnar part 421 is fixed to the first handle 12, and the other end is coaxially inserted and connected to the side of the limiting disc part 422. The diameter of the limiting disc part 422 is larger than the diameter of the columnar part 421.

[0075] When the fingers apply force to bring the two handles close together to a certain distance, the free end of the hook 41 can be looped onto the columnar part 421. Then, the limiting disc part 422 can be inserted into the columnar part 421 to prevent the hook 41 from slipping off the columnar part 421. Slowly release the force of the hand. The two handles may move slightly in opposite directions. This action can further tighten the hook 41. At this time, the two handles will no longer move in opposite directions. At this time, the inserting plate can begin. After the inserting plate is finished, pull off the limiting disc part 422. The hook 41 can then be slid to the free end of the columnar part 421 and removed. After that, the return clamping operation of the clamping spring 11 and subsequent experiments can be carried out normally.

[0076] In this embodiment, since a set of binding fasteners 4 is set on the front side of the handle for corresponding operations, in order to prevent the two front sides of the handle from flipping in opposite directions when the binding fasteners 4 are set on one side to fix the handle, which may cause the two ends of the clip spring 11 to flip forward and fail to form a stable arched structure, it is preferable that a set of binding fasteners 4 be set on the front and rear sides of the handle for relative positioning. Example 3

[0077] Since the immunohistochemical staining section fixation device 1 proposed in Embodiment 1 of this application is a small-volume experimental instrument, and each laboratory is usually equipped with more than one, it is easy to lose it if it is left randomly on the laboratory table after use, resulting in significant losses.

[0078] To address the aforementioned issues, this embodiment discloses a storage box 5 specifically designed for the immunohistochemical staining section fixation device. Referring to Figure 15, the storage box 5 includes a box body 51, drawers 52, and handles 53. The box body 51 contains five pull-out drawers 52. A handle 53 is located on the top of the box body 51 for easy lifting and moving of the entire box. Each drawer has a recessed handle on its outer side for easy pulling out of each drawer 52. A clamp 54 is located on both sides of the bottom surface inside each drawer 52. When the section fixation device is placed inside the drawer 52, the first handle 12 and the second handle 13 can be secured with the corresponding clamp 54. This design prevents the fixation device from shaking inside and causing damage. Each drawer 52 is used to store one immunohistochemical staining section fixation device 1.

[0079] In practical use, it was found that when the storage box 5 is pulled up or subjected to an external force, the drawer 52 may accidentally slip out of the storage box 5 without human intervention, causing damage to the drawer 52 and / or the immunohistochemical staining slide fixation device 1 stored inside. To prevent this, in this embodiment, a magnetic block 55 is provided on the outer side of the drawer 52 away from the handle (refer to Figure 16), and a magnet 56 is provided on the inner side of the storage box body 51 at a position corresponding to the magnetic block 55. The magnetic block 55 and the magnet 56 can be magnetically attracted to each other. This design prevents the drawer 52 from accidentally slipping out when the storage box 5 is moved or subjected to external force, which is more conducive to the preservation of the fixation device and avoids accidental damage to experimental instruments. Example 4

[0080] Immunohistochemical staining requires mounting at the end. However, xylene residue is usually present on the slides before mounting. If the slides are not properly handled (e.g., placed directly on a shelf or with paper underneath for mounting), excess xylene will inevitably leak out. Xylene is a colorless, transparent, and volatile chemical. Over time, a large amount of xylene residue will remain in the laboratory environment, seriously polluting it and affecting the health of laboratory personnel.

[0081] To address the aforementioned issues, this embodiment discloses a special mounting filter box 6, applicable to immunohistochemical staining processes, as shown in Figures 17-19. It includes a filtrate box 61, U-shaped grooves 62, and a filter screen. The filter screen comprises a filter plate 63 and support rods 64. Two support rods 64 are fixed to opposite sides of the filter plate 63, with their ends protruding beyond the filter plate 63. The filter plate 63 has several longitudinally penetrating filter holes 65 arranged in an array on both sides. The filtrate box 61 includes a box body and a lid. The lid is hinged to one side of the box body and can rotate relative to the box body to open or close it. A pair of upward-opening U-shaped grooves 62 are provided on opposite sides inside the box body. The positions of the U-shaped grooves 62 correspond to the positions of the support rods 64. After the filter screen is placed inside the box body, the protruding parts of the support rods 64 can be placed within the corresponding U-shaped grooves 62, thus suspending the filter screen.

[0082] After a series of immunohistochemical staining steps, before mounting, the slides removed from xylene, along with the immunohistochemical staining slide fixation device, are placed on a filter plate 63 for slide removal (i.e., slides are removed one by one from the fixation device). The removed slides are placed on the filter screen, and then the mounting operation is performed one by one on the filter plate 63. During the mounting process, any residual xylene on the slides can flow into the box through the filter holes 65. After all slides are mounted, the filter screen is removed from the box for professional cleaning. After removing the filter screen, the box lid is closed, and the box containing xylene can be transferred to a professional operating table for centralized processing. This method can minimize the disorderly overflow of residual xylene on the slides, reduce the impact of xylene on the laboratory environment, and ensure experimental operation and personal safety.

[0083] To prevent the filter cartridge from being accidentally opened and causing unnecessary problems, this embodiment designs a magnetic switch at the opening end of the filter cartridge 61 (refer to Figure 20). The magnetic switch includes a first magnetic element 66 on the lid and a second magnetic element 67 on the body. The first magnetic element 66 and the second magnetic element 67 can be magnetically attracted to each other, so that the filter cartridge 61 in the closed state can be tightly closed and will not be accidentally opened, causing damage to its internal filter screen and other parts. It can also prevent dust, moisture or other contaminants from entering the cartridge during normal storage, and can more effectively protect the filter cartridge itself.

[0084] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. However, the above description is merely a specific embodiment of the present invention, and the technical features of the present invention are not limited thereto. Any other embodiments derived by those skilled in the art without departing from the technical solution of the present invention should be covered within the patent scope of the present invention.

Claims

1. A laboratory immunohistochemical staining section fixation and auxiliary operation device, characterized in that, include: Immunohistochemical staining section fixation device, used to clamp and fix several sections; Storage box for storing immunohistochemical staining slide fixation devices; The immunohistochemical staining section fixation device includes a clamping spring, a first handle, and a second handle. One end of the first and second handles is fixed to both ends of the clamping spring, and the ends of the first and second handles away from the clamping spring are bent inward to form a first gripping part and a second gripping part, respectively.

2. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, The clip spring is composed of several spiral coils connected together. The cross-section of the spiral coil is square and the four corners are rounded.

3. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 2, characterized in that, The clip spring is 4-5 cm long, with an inner diameter of 1.5-2 cm, and the spring wire wound to form the clip spring has a diameter of 2-2.5 mm.

4. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, The first and second handles are fixed at an angle to both ends of the clamping spring, and the angle between the axis of the first and second handles and the axis of the clamping spring is 30°~35°.

5. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, The immunohistochemical staining section fixation device further includes a clamping and fixing component, which includes a fixing block, a first hook connector, a second hook connector, a control button, and a top pressure spring. The fixed block is fixedly connected to the first handle. The interior of the fixed block is hollow, forming a movable cavity. The fixed block has a hook-and-connect movable port on the side near the second handle, which is connected to the movable cavity. The top compression spring is located inside the movable cavity, with one end fixed to the bottom surface of the movable cavity and the other end fixedly connected to the bottom surface of the first hook connector; The top of the first hook connector is provided with a first barb that extends upward and toward the first handle at one end near the second handle, and the top surface of the first hook connector is fixedly connected to the bottom surface of the control button; One end of the second hook is fixedly connected to the second handle, and the other end is provided with a second barb that extends downward and toward the direction of the second handle.

6. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 5, characterized in that, The bottom surface of the second hook is higher than the bottom surface of the first hook and lower than the top surface of the first hook, and the first barb and the second barb can be matched and hooked.

7. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, The immunohistochemical staining section fixation device also includes a binding and fixing component, which includes hooks and fixing posts; The hook strap is a loop-shaped, inelastic connecting strap, with one end of the hook strap fixed to the second handle; The fixed post includes a columnar part at the bottom and a limiting disc part at the top. One end of the columnar part is fixed to the first handle, and the other end is inserted and connected to the side of the limiting disc part. The diameter of the limiting disc is larger than the diameter of the columnar part.

8. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, The storage box includes a storage box body, drawers, and handles. The storage box body has several pull-out drawers inside. The top of the storage box body has a handle, and each drawer has a pull handle on the outside. There is a snap-fit ​​component on each side of the bottom surface inside the drawer. The first handle and the second handle can be snapped and fixed in the storage box body using the snap-fit ​​component.

9. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 8, characterized in that, A magnetic block is provided on the outer side of the drawer at the end away from the handle, and a magnet is provided on the inner side of the storage box at the position corresponding to the magnetic block. The magnetic block and the magnet can attract each other magnetically.

10. The laboratory immunohistochemical staining section fixation and auxiliary operation device as described in claim 1, characterized in that, It also includes a special filter box for mounting, used to filter and collect residual liquid on the slides during the mounting process; The sealing filter box includes a filtrate box, a U-shaped groove, and a filter screen; The filter screen includes a filter screen plate and two support rods. The two support rods are fixed on opposite sides of the filter screen plate and the ends of the rods protrude from the filter screen plate. The filter screen plate is provided with several longitudinal filter holes that penetrate both sides of it. The filtrate box includes a box body and a box lid. The box lid is hinged to one side of the box body and can rotate relative to the box body. The box body and the box lid are opened and closed by a magnetic switch. The height of the box body is 2~2.5cm, the length is 10~11cm, and the width is 8~9cm. On opposite sides inside the box, there are a pair of U-shaped grooves with upward openings. The positions of the U-shaped grooves correspond to the positions of the support rods. After the filter screen is placed inside the box, the support rods protruding from its edges can be placed in the corresponding U-shaped grooves.

Images