Smearing apparatus and smearing method

Abstract

The present application discloses a smearing apparatus and a smearing method. The smearing apparatus comprises: a liquid driving module; a sample needle, the liquid driving module being connected to one end of the sample needle by means of a conduit, and the liquid driving module being used for driving a sample in the conduit and / or in the sample needle to be outputted to a needle port of the other end of the sample needle; a stage, the stage being used for bearing a slide to be subjected to smearing of a sample to be smeared; a control module, the control module being used for controlling the needle port of the sample needle to be in contact with the slide to be subjected to smearing, controlling the liquid driving module to drive the sample to be outputted from the needle port, during the process of outputting the sample from the needle port, keeping the needle port in contact with the slide to be subjected to smearing, and controlling the stage and the needle port to move relative to each other in at least one first target direction, so as to form a smeared layer of the sample on the slide to be subjected to smearing. The first target direction is parallel to an object bearing plane, which is the side of the stage facing the slide to be subjected to smearing. On the basis of the described method, the reliability of sample smearing can be effectively improved.

Description

Slicing apparatus and sliceing method

[0001] Cross-reference to related applications

[0002] This application claims priority to Chinese Patent Application No. 2024108679298, filed on June 28, 2024, entitled “Apparatus and Method for Spreading Patch,” the entire contents of which are incorporated herein by reference.

[0003] This application claims priority to Chinese Patent Application No. 2024108779760, filed on June 28, 2024, entitled “Apparatus and Method for Preparing Blood Film”, the entire contents of which are incorporated herein by reference.

[0004] This application claims priority to Chinese Patent Application No. 2024108679334, filed on June 28, 2024, entitled “Apparatus and Method for Generating Blood Membrane”, the entire contents of which are incorporated herein by reference.

[0005] This application claims priority to Chinese Patent Application No. 2024108780043, entitled “Coating Apparatus and Coating Method”, filed on June 28, 2024, the entire contents of which are incorporated herein by reference. Technical Field

[0006] This application relates to the field of sample testing technology, and in particular to smearing apparatus and smearing methods. Background Technology

[0007] In existing technologies, it is usually necessary to prepare a sample smear based on a slide-pushing method, using a sample and a slide to be smeared. Subsequently, the sample on the smear can be observed by a slide reading device, microscope or other observation equipment to achieve sample analysis and obtain the sample detection results.

[0008] The drawback of the push-smear method is that it typically involves dropping a certain amount of sample onto a slide (such as a glass slide), then using a pusher to press the sample and push it in one direction, causing the sample to spread out on the slide. During this process, as the pusher moves, the amount of sample in contact with it decreases. As a result, the initial layer of the sample smear formed by the pusher is too thick and has too much cell overlap, failing to meet the requirements for "monolayer cell spreading" sample detection. Conversely, the later part of the sample smear has sparse sample and a discontinuous coating, which also fails to meet the requirements for sample detection.

[0009] In the related art, a sample coating layer is formed on a slide by coating, and a sample needle is used to deliver sample liquid to the slide to form a more uniform sample coating layer than the push method. However, in the coating method of the related art, the sample needle is coated above the slide without being in contact with the slide. When the sample needle outputs too much sample liquid or at a high rate, the sample liquid may splash, and it is difficult to control the micron-level distance between the sample needle and the slide, which may also cause the sample coating layer on the sample slide to be discontinuous or have too high a cell overlap.

[0010] Therefore, the reliability of the sample slide prepared by the existing method is poor. Technical problem

[0011] How to improve the reliability of the sample slide. Technical solution

[0012] To solve the above technical problems, the first technical solution adopted by the present application is: a smearing device, comprising: a liquid driving module; a sample needle, one end of the sample needle is connected to the liquid driving module through a pipeline, and the liquid driving module is used to drive the sample in the pipeline and / or the sample needle to be output to the other end of the sample needle, i.e., the needle port; a stage, the stage is used to carry a slide to be smeared with a sample; a control module, the control module is used to control the needle port of the sample needle to be in contact with the slide to be smeared, and control the liquid driving module to drive the sample to be output from the needle port; during the process of outputting the sample from the needle port, the needle port is kept in contact with the slide to be smeared, and the stage and the needle port are controlled to move relatively in at least one first target direction to form a sample coating layer on the slide to be smeared; the first target direction is parallel to the stage plane, and the stage plane is the side of the stage facing the slide to be smeared.

[0013] Among them, the smearing device further comprises: a first moving module, the first moving module is connected to the control module; a first elastic member, the first elastic member is connected to the sample needle and the first moving module respectively, and the first moving module is used to drive the sample needle to move in a second target direction; the second target direction is perpendicular to the stage plane, and the first elastic member is used to apply an elastic pushing force or an elastic pulling force to the sample needle in the second target direction.

[0014] Among them, the smearing device further comprises: a second moving module, the second moving module is connected to the control module, and the stage is connected to the second moving module; the second moving module is used to control the stage to move in at least one first target direction.

[0015] Among them, the smearing device further comprises: a second elastic member, the second elastic member is connected to the stage and the slide to be smeared respectively, and the second elastic member is used to apply an elastic pushing force or an elastic pulling force to the stage in the second target direction; the second target direction is perpendicular to the stage plane.

[0016] The other end of the sample needle is provided with at least one groove; the groove extends in the needle body of the sample needle from the end face of the other end of the sample needle in the direction of the one end of the sample needle, and the groove is in communication with the needle port of the sample needle.

[0017] The groove has a groove width on the end face of the other end of the sample needle, and the groove width is not less than 2 microns and not more than 100 microns, and / or the groove has a groove depth in the second target direction, and the groove depth is not less than 2 microns and not more than 80 microns.

[0018] The control module is further configured to control the liquid driving module to output the sample to the needle port and make at least part of the sample form a convex liquid surface outside the needle port before the needle port of the sample needle contacts the object to be smeared; or the control module is further configured to control the liquid driving module to output the sample to the needle port and make at least part of the sample contact the object to be smeared before the stage and the needle port relatively move in the at least one first target direction.

[0019] The first target direction includes a first sub-target direction and a second sub-target direction, and the first sub-target direction is perpendicular to the second sub-target direction; the control of the stage and the needle port to relatively move in the at least one first target direction includes: controlling the stage and the needle port to relatively move in the at least one first target direction, so that the sample needle alternately performs a first relative motion and a second relative motion relative to the stage; the first relative motion is that the sample needle moves a first preset distance in the first sub-target direction relative to the stage, and then the sample needle moves a second preset distance in the second sub-target direction relative to the stage; the second relative motion is that the sample needle moves the first preset distance in the opposite direction of the first sub-target direction relative to the stage, and then the sample needle moves the second preset distance in the second sub-target direction relative to the stage.

[0020] The second preset distance is the sum of the diameter of the needle port of the sample needle and a preset diffusion distance.

[0021] The control module is further configured to: control the projection of the sample to be smeared and the projection of the needle mouth to be overlapped in the second target direction, and then control the liquid driving module to deliver the cleaning liquid to the sample needle to clean the sample needle.

[0022] The control module is further configured to: control the projection of the sample to be smeared and the projection of the needle mouth to be overlapped in the second target direction, and then control the liquid driving module to deliver the cleaning liquid to the sample needle to clean the sample needle.

[0023] The liquid driving module comprises: a first liquid driving unit configured to deliver the sample to the sample needle; and a second liquid driving unit configured to deliver the cleaning liquid to the sample needle.

[0024] The control module is further configured to: control the liquid driving module to output the sample before controlling the stage and the needle mouth to move relative to each other in the at least one first target direction, so that the sample invades the needle mouth of the sample needle.

[0025] The smearing device further comprises a cleaning swab module, and the control module is further configured to: control the cleaning swab module to absorb part of the sample located outside the sample needle at the needle mouth of the sample needle after the liquid driving module outputs the sample to make the sample invade the needle mouth of the sample needle.

[0026] The control module is further configured to: control the liquid driving module to output the sample before controlling the stage and the needle mouth to move relative to each other in the at least one first target direction, so that the sample invades the needle mouth of the sample needle.

[0027] Before the needle port of the sample needle and the object to be smeared are relatively displaced in the first target direction, the sample is outputted by the sample driving module so as to infiltrate the needle port of the sample needle, including: before the needle port of the sample needle contacts the object to be smeared, the sample is outputted by the sample driving module so as to infiltrate the needle port of the sample needle and form a convex liquid surface outside the sample needle.

[0028] Before the needle port of the sample needle and the object to be smeared are relatively displaced in the first target direction, the sample is outputted by the sample driving module so as to infiltrate the needle port of the sample needle, including: before the needle port of the sample needle contacts the object to be smeared, the sample is outputted by the sample driving module so as to infiltrate the needle port of the sample needle and form a convex liquid surface outside the sample needle.

[0029] The ratio of the length of the sample coating layer in the length direction of the object to be smeared to the length of the object to be smeared is greater than 1 / 2.

[0030] After the relative movement of the object table and the needle port in at least one first target direction is controlled to form a sample coating layer on the object to be smeared, the control module is further used for: first controlling the sample driving module to suck the sample through the sample needle, and then controlling the needle port of the sample needle to move away from the object to be smeared.

[0031] The sample driving module includes: a first sample driving unit for delivering the sample to the sample needle; and a second sample driving unit for sucking the sample through the sample needle; the flow rate of the sample delivered by the first sample driving unit is less than the flow rate of the sample sucked by the second sample driving unit.

[0032] To solve the above technical problems, the second technical scheme adopted by the present application is: a smearing method applied to the smearing device; the smearing method includes: controlling the needle port of the sample needle to contact the object to be smeared, and controlling the sample driving module to drive the sample to be outputted from the needle port; during the outputting of the sample from the needle port, the needle port is kept in contact with the object to be smeared, and the relative movement of the object table and the needle port in at least one first target direction is controlled to form a sample coating layer on the object to be smeared. Beneficial effects

[0033] Differing from the prior art, in the technical solution of the application, the liquid driving module is used to deliver the sample to the sample needle to output from the needle port of the sample needle, the object table is used to carry the to-be-smear, and the control module is used to keep the needle port of the sample needle in contact with the to-be-smear during the output of the sample from the needle port of the sample needle, and control the relative movement of the object table and the needle port of the sample needle in at least one first target direction, so as to realize the smearing of the sample on the to-be-smear and form a sample coating on the to-be-smear. Based on the above-mentioned mode, firstly, the sample can be smeared on any position on the sample smear during the relative movement, so that the sample forms a uniform coating of any shape required by the user on the to-be-smear, thereby reducing the possibility that the coating formed on the to-be-smear cannot meet the requirements of sample detection due to the over-thickness of the coating and the over-high cell overlapping degree of part sections, and / or due to the reasons of the sample scarcity and the discontinuous coating of part sections. Secondly, by keeping the needle port of the sample needle in contact with the to-be-smear for smearing, the possibility of the splashing of the sample liquid can be reduced, the distance between the needle port of the sample needle and the to-be-smear does not need to be controlled, the sample coating formed is uniform and smooth, and is regular and uninterrupted. In summary, the reliability of the sample smear is improved. BRIEF DESCRIPTION OF DRAWINGS

[0034] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0035] FIG. 1 is a structural schematic diagram of an embodiment of the smear device of the present application;

[0036] FIG. 2 is a schematic diagram of an embodiment of the to-be-smear and the object table of the present application;

[0037] FIG. 3 is a schematic diagram of an embodiment of the to-be-smear and the object table of the present application;

[0038] FIG. 4 is a schematic diagram of an embodiment of the to-be-smear and the object table of the present application;

[0039] FIG. 5 is a schematic diagram of an embodiment of the to-be-smear and the object table of the present application;

[0040] FIG. 6 is a bottom view schematic diagram of an embodiment of the needle port of the sample needle of the present application;

[0041] FIG. 7 is a side view schematic diagram of an embodiment of the needle port of the sample needle of the present application;

[0042] FIG. 8 is a schematic diagram of an embodiment of the sample needle and the sample in the sample needle of the present application;

[0043] Fig. 9 is a second schematic view of an embodiment of a sample needle and a sample therein according to the present application;

[0044] Fig. 10 is a third schematic view of an embodiment of a sample needle and a sample therein according to the present application. DETAILED DESCRIPTION

[0045] The present application will be further described below in conjunction with the accompanying drawings and embodiments. It is particularly pointed out that the following embodiments are only for illustrating the present application, but not for limiting the scope of the present application. Similarly, the following embodiments are only part of the embodiments of the present application, but not all the embodiments of the present application, and all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0046] In this document, reference to“an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of other embodiments. Those skilled in the art will appreciate that the embodiments described herein are merely examples of the application and that variations of these embodiments can be used and that it is in the intent of the application that combinations of these embodiments with other embodiments and variations of these embodiments can be used.

[0047] In the description of the present application, it is to be noted that unless otherwise explicitly specified and limited, the terms“mounting”,“setting”,“connecting”,“connecting” should be understood broadly, for example, it can be fixedly connected, it can be detachably connected, or integrally connected; it can be mechanically connected, or electrically connected; it can be directly connected, or connected through an intermediate medium. For those skilled in the art, the above can be specifically connected according to the specific meaning of the present application.

[0048] The present application provides a smearing device, as shown in Fig. 1, which is a structural schematic view of an embodiment of the smearing device according to the present application. As shown in Fig. 1, the smearing device comprises a liquid driving module 11, a sample needle 12, a carrier table 13 and a control module (not shown in the figure).

[0049] The liquid driving module 11 is connected to one end of the sample needle 12 through a pipeline. The liquid driving module 11 is used to drive the sample in the pipeline and / or in the sample needle 12 to be output to the needle opening at the other end of the sample needle 12.

[0050] The liquid driving module 11 can be used to store blood samples and other types of samples, which are not limited here. The liquid driving module 11 can further comprise a power mechanism connected to the sample needle 12 to transport the stored sample to the sample needle 12, so that the sample is output from the end of the sample needle 12 close to the to-be-smearing sheet 21.

[0051] The carrier table 13 is used to carry the to-be-smearing sample, i.e. the to-be-smearing sheet 21.

[0052] The to-be-coated slide 21 can be a glass slide, for example. The sample can be applied to the to-be-coated slide 21 to obtain a sample glass slide, which can be used for observation to obtain a sample detection result.

[0053] The to-be-coated slide 21 can be placed on the stage 13, and the side of the to-be-coated slide 21 on which the sample is to be applied faces away from the stage 13, so that the sample needle 12 can output the sample to the to-be-coated slide 21 for subsequent coating operations.

[0054] The control module is configured to:

[0055] The control module is configured to:

[0056] During the continuous output of the sample from the needle opening, the needle opening is kept in contact with the to-be-coated slide 21, and the stage 13 and the needle opening are controlled to move relative to each other in at least one first target direction D1 to form a sample coating layer on the to-be-coated slide 21.

[0057] The first target direction D1 is parallel to the loading plane, which is the side of the stage 13 facing the to-be-coated slide 21. The first target direction D1 can be any direction on the loading plane. The first target direction D1 in any direction can be used as a starting point for the relative movement, and the first target direction D1 in any other direction can be used for subsequent relative movement. The specific direction of the first target direction D1 is not limited herein.

[0058] Regarding the control module, first, the control module can be a module having the ability to drive the stage 13 and the needle opening of the sample needle 12 to move relative to each other in the first target direction D1 while keeping the needle opening in contact with the to-be-coated slide 21. The control module can be any type of module having driving ability, and the specific type of module can be determined according to actual needs. The specific type of module is not limited herein.

[0059] In an example, the control module can include or be connected to a corresponding movement module. The movement module can be a module for moving the sample needle 12 and / or the stage 13, such as a motor drive mechanism and a corresponding slide rail. The movement module can also be another type of mechanism having the ability to move the sample needle 12 and / or the stage 13. The specific type of mechanism can be determined according to actual needs. The specific type of mechanism is not limited herein.

[0060] For example, as shown in the view of Fig. 1, when the sample needle 12 is controlled to keep the sample outlet end in contact with the slide 21 and to move the sample outlet end of the sample needle 12 relative to the slide 21 in at least one first target direction D1, the second target direction D2 is perpendicular to the slide plane, and the second target direction D2 can be upward or downward, and the first target direction D1 can be forward or backward, left or right, or any target direction parallel to the surface of the slide 21 carrying the sample, which is not limited here.

[0061] As shown in the view of Fig. 1, in the first example, the control module can control the sample needle 12 to move up and down in the second target direction D2 and control the stage 13 to move forward and backward and left and right in at least one first target direction D1 through the moving module.

[0062] In the second example, the control module can control the sample needle 12 to move forward and backward and left and right in at least one first target direction D1 and control the stage 13 to move up and down in the second target direction D2 through the moving module.

[0063] In the third example, the control module can control the stage 13 to be static and control the sample needle 12 to move up and down in the second target direction D2 and move forward and backward and left and right in at least one first target direction D1 through the moving module.

[0064] In the fourth example, the control module can control the sample needle 12 to be static and control the stage 13 to move up and down in the second target direction D2 and move forward and backward and left and right in at least one first target direction D1 through the moving module.

[0065] Secondly, the control module can also have the ability to control the liquid driving module 11 to deliver the sample to the sample needle 12, and / or the control ability of the control module to control other devices of the smearing device to work, which is not limited here.

[0066] It can be understood that the up and down movement in the second target direction D2 is to make the sample needle 12 contact the slide 21, and the forward and backward and left and right movement in the first target direction D1 is to form a sample coating on the slide 21.

[0067] Specifically, the above-mentioned liquid driving module 11 can specifically refer to a syringe, the range of which is not less than 30 microliters and not greater than 100 microliters. Since the sample coating usually only needs 2-5 microliters or other microliter-level total amount of sample, by configuring the range of the syringe to a smaller interval, the control accuracy of the sample delivery speed during subsequent sample delivery can be improved, thereby reducing the possibility of the situation that the cell overlap is too high due to outputting too much sample to the to-be-coated slide 21, or the situation that the sample coating is discontinuous due to outputting too little sample to the to-be-coated slide 21, thereby facilitating the preparation of the single-layer cell flat sample coating, and improving the reliability of the sample coating.

[0068] In an example, a reverse R corner can be arranged at the edge of the needle opening of the sample needle 12, and / or the end face of the other end of the sample needle 12 is a plane, thereby reducing the contact stress between the needle opening and the to-be-coated slide 21, so as to reduce the possibility of scratches and other damage to the to-be-coated slide 21 and damage to the sample cells during the coating process.

[0069] The liquid driving module 11 can obtain the sample through the sample needle 12. In a first mode, the liquid driving module 11 can control the sampling needle to transfer the sample to the sample needle 12 after the sampling needle pierces into the sample tube and collects the sample. The sampling needle and the sample needle 11 can be independent double needles or integrated single needles. In a second mode, the liquid driving module 11 can control the sample needle 12 to collect the sample from the sample tube after the piercing needle pierces the cap of the sample tube to form an opening and the sample needle 12 enters the sample tube through the opening. The piercing needle and the sample needle 11 can be independent double needles or integrated single needles. Through the two modes, the sample can be obtained.

[0070] Based on the above-mentioned mode, the needle opening can move relative to the to-be-coated slide 21 according to the preset track when the stage 13 and the needle opening move relative to each other in at least one first target direction D1, so that the sample output from the needle opening forms a single-layer cell sample coating with a preset shape on the to-be-coated slide 21. The preset shape can be determined according to actual needs, for example, it can be a rectangle, a circle, or any shape required by a user, which is not limited here.

[0071] Compared with the traditional pushing method, the technical scheme of the present application can perform sample coating in the same way at any position on the to-be-coated slide 21, so that the cell distribution at each position in the sample coating formed is relatively uniform, and under the condition that the relationship between the relative movement speed and the sample delivery speed is reasonably configured, the cell overlap and the sample content at each position in the sample coating of the sample slide formed are relatively uniform, and the possibility of the situation that the coating is too thick or the coating is discontinuous can be reduced as much as possible, thereby improving the reliability of the sample slide.

[0072] In addition, by keeping the needle opening in contact with the slide to be smeared 21 during the output of the sample, the possibility of sample splashing when the sample delivery speed is too large or serious cell overlapping or discontinuous coating when the sample delivery speed is too small can be reduced, and the distance between the needle opening of the sample needle and the slide to be smeared does not need to be controlled, the sample coating formed is uniform, flat, regular and uninterrupted, and the reliability of sample smearing is further improved.

[0073] Unlike the prior art, in the technical solution of the present application, the liquid driving module is used to deliver the sample to the sample needle for output from the needle opening of the sample needle, the sample stage is used to carry the slide to be smeared, and the control module is used to keep the needle opening of the sample needle in contact with the slide to be smeared during the continuous output of the sample from the needle opening of the sample needle, and to control the relative movement of the sample stage and the needle opening of the sample needle in at least one first target direction, to realize the smearing of the sample on the slide to be smeared, and to form a sample coating on the slide to be smeared. Based on the above-mentioned manner, first, the sample can be smeared on any position on the sample slide during the relative movement, so as to form a uniform coating of any shape required by the user on the slide to be smeared, and the possibility that the coating formed on the slide to be smeared cannot meet the requirements of sample detection due to the over-thickness of the coating and the over-high degree of cell overlapping in some sections, and / or due to the lack of sample and discontinuous coating in some sections is reduced. Secondly, by keeping the needle opening of the sample needle in contact with the slide to be smeared for coating, the possibility of sample liquid splashing is also reduced, the distance between the needle opening of the sample needle and the slide to be smeared does not need to be controlled, and the sample coating formed is uniform, flat, regular and uninterrupted. In summary, the reliability of sample smearing is improved. In an embodiment, as shown in FIG. 1, the smearing device further comprises a first moving module 14 and a first elastic member 15.

[0074] The first moving module 14 is connected to the control module.

[0075] The first elastic member 15 is connected to the sample needle 12 and the first moving module 14 respectively, and the first moving module 14 is used to drive the sample needle 12 to move in a second target direction D2.

[0076] The second target direction D2 is perpendicular to the sample plane, and the first elastic member 15 is used to apply an elastic pushing force or an elastic pulling force in the second target direction D2 to the sample needle 12.

[0077] Specifically, the smearing device can further include a main body, and the first moving module 14 can be a module arranged on the main body and capable of causing the sample needle 12 to move downward or upward or other types of movement in the second target direction D2. For example, a first sliding rail extending in the second target direction D2 can be arranged on the main body, and the first moving module 14 can cause the part of the mechanism connected to the sample needle 12 to move on the first sliding rail. In other examples, the first moving module 14 can also be other forms of modules having the above-mentioned capability, which are not limited here.

[0078] An outer flange can be arranged on the outer side wall of the sample needle 12, one end of the first elastic member 15 can be connected to the outer flange on the sample needle 12, and the other end of the first elastic member 15 can be connected to the first moving module 14, that is, the first moving module 14 is connected to the sample needle 12 through the first elastic member 15, so that the sample needle 12 can move within a certain range in the second target direction D2 due to the elastic deformation of the first elastic member 15 even when the first moving module 14 stops working under the action of an external force. Since the to-be-smearing sample 21 and the needle opening cannot be absolutely smooth and have a certain degree of unevenness, based on the above-mentioned manner, the sample needle 12 can be caused to keep contact with the to-be-smearing sample 21 based on the elastic force when the needle opening of the sample needle 12 does not descend far enough, thereby reducing the possibility of sample spraying, and the contact stress between the needle opening of the sample needle 12 and the to-be-smearing sample 21 can be reduced based on the elastic force when the needle opening of the sample needle 12 descends too far, thereby reducing the possibility of damage such as scratching of the to-be-smearing sample 21 by the needle opening of the sample needle 12, and improving the reliability of sample smearing.

[0079] In practice, the sample needle 12 can be controlled to descend to a degree at which the first elastic member 15 slightly compresses and deforms to generate an elastic force, so that the first elastic member 15 can abut the sample needle 12 against the to-be-smearing sample 21 through the elastic force, thereby realizing stable contact between the sample needle 12 and the to-be-smearing sample 21 during smearing, and improving the stability of smearing. In this process, the first elastic member 15 can play a buffering role when the sample needle 12 contacts the to-be-smearing sample 21, thereby reducing the possibility of damage to the to-be-smearing sample 21 by the sample needle 12 due to too large a distance moved by the sample needle 12 toward the to-be-smearing sample 21, and improving the fault tolerance and safety when the sample needle 12 is controlled to move toward the to-be-smearing sample 21 to realize contact therebetween.

[0080] Based on the above-mentioned manner, the needle opening of the sample needle 12 can be controlled to keep contact with the to-be-smearing sample 21 by the first moving module 14, thereby reducing the possibility of sample spraying when the sample conveying speed is too large, or the possibility of serious cell overlapping or discontinuous smearing when the sample conveying speed is too small, and further improving the reliability of sample smearing.

[0081] In an embodiment, as shown in FIG. 1, the smearing device further includes a second moving module 16.

[0082] The second moving module 16 is connected to the control module, and the object table 13 is connected to the second moving module 16, and the second moving module 16 is used to control the object table 13 to move in at least one first target direction D1.

[0083] Specifically, the smearing device can further include a main body, and the second moving module 16 can be a module arranged on the main body and capable of moving the object table 13 forward and backward or left and right or other types of movement in the first target direction D1, for example, referring to FIGS. 2-5, FIG. 2 is one of the schematic diagrams of an embodiment of the smearing device and the object table, FIG. 3 is another of the schematic diagrams of an embodiment of the smearing device and the object table, FIG. 4 is a third of the schematic diagrams of an embodiment of the smearing device and the object table, and FIG. 5 is a fourth of the schematic diagrams of an embodiment of the smearing device and the object table, as shown in FIGS. 2-5, the trajectory formed by the arrows in the smearing object 21 is the preset trajectory, and the at least one first target direction D1 includes a first sub-target direction D11 and a second sub-target direction D12, and the first sub-target direction D11 and the second sub-target direction D12 are perpendicular to each other.

[0084] In the view shown in FIG. 1, the output end of the sample needle 12 can move forward and backward relative to the smearing object 21 in the first sub-target direction D11, and / or move left and right in the second sub-target direction D12, wherein, as shown in FIGS. 2-5, the first sub-target direction D11 can be a forward direction or a backward direction, and the second sub-target direction D12 can be a left direction or a right direction, which is not limited here.

[0085] The second moving module 16 can control the object table 13 to move in the first sub-target direction D11 and the second sub-target direction D12, respectively, so that the object table 13 and the needle port of the sample needle 12 produce relative movement in the first sub-target direction D11 and the second sub-target direction D12, respectively, to enable the sample output from the needle port to smear on the smearing object 21 according to the above-mentioned preset trajectory.

[0086] It should be noted that in other embodiments, the first moving module 14 described in the foregoing embodiments can control the sample needle 12 to move forward and backward relative to the smearing object 21 in the first sub-target direction D11, and / or move left and right in the second sub-target direction D12, to enable the sample output from the needle port to smear on the smearing object 21 according to the above-mentioned preset trajectory, or the first moving module 14 described in the foregoing embodiments and the second moving module 16 described in the foregoing embodiments can be controlled respectively to enable the sample output from the needle port to smear on the smearing object 21 according to the above-mentioned preset trajectory, which is not limited here.

[0087] In an embodiment, as shown in FIG. 1, the smearing device further comprises a second elastic member 17.

[0088] The second elastic member 17 is connected to the object table 13 and the to-be-smearing slide 21 respectively, and is used to apply an elastic pushing force or an elastic pulling force in a second target direction D2 to the object table 13.

[0089] The second target direction D2 is perpendicular to the object plane.

[0090] Specifically, one end of the second elastic member 17 can be connected to the object table 13, and the other end of the second elastic member 17 can be connected to the to-be-smearing slide 21, that is, the object table 13 is connected to the to-be-smearing slide 21 through the second elastic member 17, so that the to-be-smearing slide 21 can perform a certain range of buffering motion in the second target direction D2 due to the elastic deformation of the second elastic member 17 even in the case that the first moving module 14 controls the sample needle 12 to contact the to-be-smearing slide 21 and the moving distance of the downward movement is too large and a larger external force is applied. Based on the above manner, when the needle port of the sample needle 12 is lowered too much, the contact stress between the needle port and the to-be-smearing slide 21 can be reduced based on the elastic force, thereby reducing the possibility of damage such as scratching of the to-be-smearing slide 21 caused by the needle port of the sample needle 12, and improving the reliability of sample smearing.

[0091] In practice, the sample needle 12 can be controlled to be lowered to a degree that the second elastic member 17 slightly compresses and deforms to generate an elastic force, so that the second elastic member 17 can abut the sample needle 12 on the to-be-smearing slide 21 through the elastic force, to realize stable contact between the sample needle 12 and the to-be-smearing slide 21 in the smearing process, thereby improving the stability of smearing. In this process, through the setting of the second elastic member 17, the buffering effect can be achieved when the sample needle 12 contacts the to-be-smearing slide 21, to reduce the possibility of damage to the to-be-smearing slide 21 caused by the sample needle 12 due to the too large moving distance of the sample needle 12 to the to-be-smearing slide 21, and to improve the fault tolerance rate and safety when the sample needle 12 is controlled to move to the to-be-smearing slide 21 to realize contact therebetween.

[0092] In an embodiment, referring to FIGS. 6 and 7, FIG. 6 is a bottom view of an embodiment of the needle port of the sample needle, and FIG. 7 is a side view of an embodiment of the needle port of the sample needle. As shown in FIGS. 6 and 7, the other end of the sample needle 12 is provided with at least one groove 121.

[0093] The groove 121 extends in the direction of one end of the sample needle 12 from the end face of the other end of the sample needle 12 in the needle body of the sample needle 12, and the groove 121 is in communication with the needle port X of the sample needle 12.

[0094] Specifically, as shown in FIG. 6, the accommodating space in the groove 121 is in communication with the space for conveying the sample corresponding to the needle opening X, and the sample can flow out of the groove 121. As shown in FIG. 7, the groove 121 can extend in the needle body of the sample needle 12 from the end face of the other end of the sample needle 12 in the direction of one end of the sample needle 12 to form a groove with a certain depth.

[0095] Based on the above manner, although the needle opening is kept in contact with the object slide 21 when the sample is output from the needle opening, there is still a gap between the needle opening and the object slide 21 in a microscopic view. During the sample coating process, the cells in the sample can flow out of the gap. Without the groove 121, larger cells (such as white blood cells in the sample, which are usually larger in size) or cell clusters gathered together in the sample are easily squeezed and broken by the gap, resulting in distortion of the sample detection result. By providing the above groove 121, the larger cells in the sample can flow out of the groove 121, reducing the possibility of cell breakage and further improving the reliability of sample smearing.

[0096] In addition, when cells are gathered, the gathered cells can also be output without damage through the groove, reducing the possibility of being squeezed and broken by the smaller gap between the sample needle 12 and the object slide 21 due to the overall volume of the gathered cells being too large, and further improving the reliability of sample smearing.

[0097] In addition, since the needle opening of the sample needle 12 is kept in contact with the object slide 21 for coating, if the sample flow rate is too large, the sample may splash due to ineffective release of hydraulic pressure. By providing the above groove 121, the hydraulic pressure can be effectively released in this case, reducing the possibility of sample splashing and improving the stability during coating, and further improving the reliability of sample smearing.

[0098] Optionally, the groove width of the groove 121 on the end face of the other end of the sample needle 12 is not less than 2 microns and not more than 100 microns, and / or the groove depth of the groove 121 in the second target direction D2 is not less than 2 microns and not more than 80 microns.

[0099] Specifically, as shown in FIGS. 6 and 7, the groove width can be S1, and the groove depth can be S2. By making the groove width S1 not less than 2 microns and not more than 100 microns, and / or the groove depth S2 of the groove 121 in the second target direction D2 not less than 2 microns and not more than 80 microns, the situation that larger cells or gathered cell clusters are squeezed and broken due to the gap being too small can be avoided, thereby improving the reliability of sample smearing.

[0100] It should be noted that the at least one groove 121 can be arranged at intervals around the center of the needle port, and the number of the at least one groove 121 can be determined according to requirements, which is not limited here. In addition, since the size of the largest white blood cell is generally not less than 7 microns and not more than 20 microns, the groove width S1 and the groove depth S2 can also be set to a size that is not much different from the size of the white blood cell, such as can be set to not less than 7 microns and not more than 20 microns, to ensure that the white blood cell can flow out smoothly without being damaged and reduce the possibility of sample splashing, further improving the reliability of sample smearing.

[0101] In an embodiment, the control module is further configured to:

[0102] Before the needle port of the sample needle 12 contacts the to-be-smearing 21, the control liquid driving module 11 outputs the sample to the needle port, and at least part of the sample forms a convex liquid surface outside the needle port;

[0103] Alternatively, the control module is further configured to control the liquid driving module 11 to output the sample to the needle port before the stage 13 and the needle port move relative to each other in at least one first target direction D1, and at least part of the sample contacts the to-be-smearing 21.

[0104] Specifically, referring to FIGS. 8-10, FIG. 8 is a schematic diagram of an embodiment of the sample needle and the sample therein, FIG. 9 is a schematic diagram of an embodiment of the sample needle and the sample therein, and FIG. 10 is a schematic diagram of an embodiment of the sample needle and the sample therein.

[0105] For example, as shown in FIG. 8, which is a schematic diagram of a sample needle 12 and the sample therein without forming a convex liquid surface outside the needle port, it can be seen that the sample forms a concave liquid surface Z1 in the sample needle 12. If the needle port contacts the to-be-smearing 21 and the output of the sample is controlled at this time, the sample coating on the to-be-smearing 21 is likely to be discontinuous due to the presence of air between the concave liquid surface Z1 and the to-be-smearing 21, that is, the sample cannot form a continuous coating due to the presence of air, which further easily leads to distortion of the sample detection result.

[0106] However, based on the technical solution of the present application, as shown in FIGS. 9 and 10, a convex liquid surface Z2 or Z3 can be formed outside the needle port, and then the needle port is controlled to contact the to-be-smearing 21, so that at least part of the sample contacts the to-be-smearing 21, and then the output of the sample is performed, which can reduce the possibility of the problem as shown in the example of FIG. 8, and improve the reliability of sample smearing.

[0107] In addition, the distance between the farthest end of the convex liquid surface away from the needle port and the needle port has a negative correlation with the viscosity of the sample, and the distance can be controlled by the liquid driving module 11 according to the empirical value of the average viscosity of the sample.

[0108] If the viscosity of the sample is large, the amount of liquid protruding out of the needle 12 at the needle opening can be small, and if the viscosity of the sample is small, the amount of liquid protruding out of the needle 12 at the needle opening can be large. For example, FIG. 9 shows a schematic diagram of a sample with large viscosity and the needle 12, and the maximum distance between the protruding liquid surface Z2 and the needle opening of the needle 12 is T1. FIG. 10 shows a schematic diagram of a sample with small viscosity and the needle 12, and the maximum distance between the protruding liquid surface Z3 and the needle opening of the needle 12 is T2. T1 is smaller than T2.

[0109] Based on the above manner, the size of the protruding liquid surface to be formed can be adapted to the viscosity of the sample, the possibility of the liquid surface being damaged and the sample leaking out due to the formation of a protruding liquid surface that is not adapted can be reduced, the possibility of the leaked sample polluting the object to be smeared 21 or the device can be reduced, and the reliability of sample smearing can be improved.

[0110] Optionally, the smearing device further comprises a cleaning swab module, the cleaning swab module being sleeved on the needle 12.

[0111] Before the needle opening of the needle 12 contacts the object to be smeared 21, the control liquid driving module 11 outputs the sample to the needle opening, and after at least part of the sample forms a protruding liquid surface outside the needle opening, the control module is further configured to:

[0112] control the cleaning swab module to suck the part of the sample outside the needle opening, so that the maximum distance between the protruding liquid surface and the needle opening of the needle 12 is reduced.

[0113] Specifically, since it is difficult to determine the viscosity of the sample, when the user cannot determine the viscosity of the sample, the sample can be output to form a protruding liquid surface, and then the cleaning swab module is controlled to suck a part of the sample outside the needle opening corresponding to the protruding liquid surface, so as to reduce the sample corresponding to the protruding liquid surface, so that the protruding liquid surface is reduced to a more appropriate size, the possibility of the liquid surface being damaged and the sample leaking out due to the formation of a protruding liquid surface that is not adapted can be reduced, the possibility of the leaked sample polluting the object to be smeared 21 or the device can be reduced, and the reliability of sample smearing can be improved.

[0114] In an embodiment, as shown in FIGS. 2 and 3, the at least one first target direction D1 includes a first sub-target direction D11 and a second sub-target direction D12, and the first sub-target direction D11 is perpendicular to the second sub-target direction D12.

[0115] The control of the relative movement of the object table 13 and the needle opening in the at least one first target direction D1 can specifically include:

[0116] The control of the stage 13 and the needle mouth to move relatively in at least one first target direction D1, so that the sample needle 12 alternately performs first relative motion and second relative motion relative to the stage 13.

[0117] The first relative motion is that the sample needle 12 moves a first preset distance in a first sub-target direction D11 relative to the stage 13, and then the sample needle 12 moves a second preset distance in a second sub-target direction D12 relative to the stage 13.

[0118] The second relative motion is that the sample needle 12 moves a first preset distance in the opposite direction of the first sub-target direction D11 relative to the stage 13, and then the sample needle 12 moves a second preset distance in the second sub-target direction D12 relative to the stage 13.

[0119] Specifically, for example, as shown in FIG. 2 or FIG. 3, when the sample is output from the needle mouth of the sample needle 12, the control of the stage 13 and the sample needle 12 to move in the first sub-target direction D11 and the second sub-target direction D12 respectively as described above, so that the sample is coated on the arc-shaped track corresponding to the above movement, and in cooperation with the diffusion phenomenon of the sample itself after being coated on the smear 21, the sample can form a sample coating layer within the rectangular frame corresponding to the track.

[0120] In addition, the sample coating layer formed by the sample moving in the first sub-target direction D11 multiple times can have no gap between the sample coating layers of adjacent rows in the row part of the sample coating layer, that is, the sample coating layers of adjacent rows partially overlap or connect, so that there is no gap between the rows in the finally formed sample coating layer, improving the quality of the formed sample coating layer, and improving the proportion of the single-layer cell paving part in the sample coating on the sample smear, so as to facilitate subsequent sample detection, thereby improving the reliability of the sample smear.

[0121] It should be noted that the second preset distance can be the sum of the outer diameter of the needle mouth and a preset diffusion distance, and the preset diffusion distance can be the width distance increased due to diffusion in one first target direction D1 from the initial time to the time when the diffusion phenomenon ends after the sample is output to the smear 21.

[0122] It should be noted that the concentration of the sample is different, and the preset diffusion distance is different. The preset diffusion distance can be set as the distance increased when the sample corresponding to the sample used to form the sample coating diffuses, or the preset diffusion distance can be set as a preset value in the preset diffusion distance interval of the commonly used sample, which is not limited here. Based on this, the samples coated according to the corresponding trajectory can be connected and matched at the edges after diffusion, thereby forming a continuous sample coating with appropriate cell overlap, for example, a single-layer cell flat sample coating can be formed, which is convenient for sample detection.

[0123] Optionally, the second preset distance is the sum of the needle port diameter of the sample needle 12 and the preset diffusion distance.

[0124] Specifically, as shown in FIG. 2 or FIG. 3 or FIG. 4 or FIG. 5, the inter-row spacing of the sample needle 12 relative to the sample slide 21 in the arc trajectory is set to the target distance by setting the second preset distance as the target distance. When the sample is coated according to the arc trajectory, a space for sample diffusion can be reserved, so that the samples between rows can be connected to each other after diffusion, so that the sample coating formed by the sample can reduce the possibility of discontinuity of the sample and reduce the possibility of excessive cell overlap of the sample, thereby improving the reliability of the sample coating.

[0125] In an embodiment, when the control stage 13 and the needle port are relatively moved in at least one first target direction D1, the speed of the relative movement is recorded as the relative movement speed.

[0126] The expected thickness of the sample on the sample slide 21 is recorded as the coating thickness.

[0127] The average diffusion speed of the sample on the sample slide 21 is recorded as the sample diffusion speed.

[0128] The total duration of the diffusion of the sample on the sample slide 21 is recorded as the sample diffusion duration.

[0129] The maximum outer diameter of the sample needle 12 in the first target direction D1 is recorded as the sample needle outer diameter.

[0130] The maximum inner diameter of the sample needle 12 in the first target direction D1 is recorded as the sample needle inner diameter.

[0131] The speed of the sample needle 12 transporting the sample is recorded as the sample transport speed.

[0132] The sample transport speed is not less than the first speed and not greater than the second speed.

[0133] The first speed is a product of the relative movement speed, the coating thickness, and a first value, the second speed is a product of the relative movement speed, the coating thickness, and a second value, the first value is a sum of a third value and an inner diameter of the sample needle 12, the second value is a sum of the third value and an outer diameter of the sample needle 12, and the third value is a product of a sample diffusion speed and a sample diffusion duration.

[0134] Specifically, as shown in FIG. 8, the outer diameter of the sample needle 12 is φout, and the inner diameter of the sample needle 12 is φin.

[0135] When the stage 13 and the needle port move relative to each other in at least one first target direction D1, the speed of the relative movement is Vrel.

[0136] The coating thickness is a thickness H of a sample coating formed on the slide 21 in a second target direction D2.

[0137] After the sample is output from the needle port to the slide 21, the sample diffuses for a certain duration, and the average diffusion speed of the diffusion is Vdiff, and the total duration from the beginning to the end of the diffusion is Tdiff.

[0138] The speed at which the sample needle 12 transports the sample is Vsample.

[0139] After the initial time at which the sample is output to the slide 21, the sample diffuses on the slide 21, and the greater the viscosity of the sample, the smaller the diffusion speed, and the smaller the viscosity of the sample, the greater the diffusion speed.

[0140] A preset amount of sample used to form a sample coating can be output on the slide 21, the width in the target direction at the initial time at which the sample is output to the slide 21 is recorded as an initial width, and the timing is started until the width of the sample in the target direction stops changing, and the timing is completed, the duration obtained by the timing is recorded as the sample diffusion duration Tdiff, and the final value of the width of the sample in the target direction is recorded as a target width.

[0141] The sample diffusion speed Vdiff is the sample diffusion width divided by the sample diffusion duration Tdiff, and the sample diffusion width is a value obtained by subtracting the initial width from the target width. The target direction is one of all the first target directions D1.

[0142] Vrel*H*(Vdiff*Tdiff+φin)≤Vsample≤Vrel*H*(Vdiff*Tdiff+φout).

[0143] By causing the sample transport speed Vsample to satisfy the above formula, the possibility of the sample coating being too thick or discontinuous can be reduced, and the reliability of the sample slide can be further improved.

[0144] For example, according to the formula, when Vexp is 0.1 mm / s, Texp is 0.1 s, phi_in is 0.3 mm, phi_out is 1.6 mm, and H is 0.01 mm, first, if Vphase is 4 mm / s, then Vsample is not less than 0.0124 μL / s and not greater than 0.0644 μL / s, second, if Vphase is 16 mm / s, then Vsample is not less than 0.0496 μL / s and not greater than 0.2576 μL / s, and third, if Vphase is 28 mm / s, then Vsample is not less than 0.0868 μL / s and not greater than 0.4508 μL / s.

[0145] The above is only an example, and other numerical combinations can also be specific, which are not limited herein.

[0146] In an embodiment, the length of the sample needle 12 is not greater than 20 cm.

[0147] Specifically, based on the above manner, when the sample needle 12 is kept in contact with the to-be-smear 21, the rigidity of the sample needle 12 can be improved by reducing the length of the sample needle 12, so that the sample needle 12 is not easy to deform or not easy to deform too much when subjected to the force applied by the to-be-smear 21, thereby enabling the sample in the sample needle 12 to be output to the desired site according to user demand, and improving the reliability of the formed sample coating.

[0148] In an embodiment, the sample needle 12 can include a coating needle and a puncture needle, and the puncture needle is sleeved outside the coating needle.

[0149] Before the needle opening of the sample needle 12 is controlled to be in contact with the to-be-smear 21 and the liquid driving module 11 is controlled to continuously drive the sample to be output from the needle opening, the control module is further configured to: control the puncture needle to puncture the sample test tube, and control the coating needle to enter the sample test tube, so that the coating needle sucks the sample in the sample test tube based on the driving force provided by the liquid driving module 11.

[0150] Controlling the needle opening of the sample needle 12 to be in contact with the to-be-smear 21 and controlling the liquid driving module 11 to continuously drive the sample to be output from the needle opening can specifically include:

[0151] Controlling the needle opening of the coating needle to be in contact with the to-be-smear 21 and controlling the liquid driving module 11 to continuously drive the sample to be output from the needle opening.

[0152] During the continuous output of the sample from the needle opening, the needle opening is kept in contact with the to-be-smear 21, and the stage 13 and the needle opening are controlled to move relatively in at least one first target direction D1 to form a sample coating on the to-be-smear 21, which can specifically include:

[0153] The needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening.

[0154] Based on the above manner, the needle for puncture and the needle for sample coating are different two needles. Since the particulate matter generated by puncture is usually adhered to the puncture needle for puncture, the possibility of the sample coating needle being contaminated by the particulate matter is low. When the sample coating is performed based on the sample coating needle, the possibility of the particulate matter falling on the slide 21 to be coated is small, thereby reducing the possibility of the particulate matter generated by puncture contaminating the sample and negatively interfering with subsequent sample detection, and improving the reliability of the sample coating. In addition, since the output amount of the sample needs to be controlled during sample coating, the inner diameter of the needle for coating is small, that is, the overall rigidity is poor. If the sample test tube is directly punctured, the coating needle is easily deformed or even broken, or the test tube cap of the sample test tube is not punctured. Therefore, the needle for puncture and the needle for sample coating are different two needles, and the strength of the puncture needle is greater than that of the coating needle.

[0155] In another embodiment, the coating needle and the puncture needle are two needles that are spatially independent and separated from each other.

[0156] Before the needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening, the control module is further configured to control the puncture needle to puncture the sample test tube, and control the puncture needle to suck the sample in the sample test tube based on the driving force provided by the drive liquid module 11.

[0157] The needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening, which can specifically include:

[0158] The needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening, which can specifically include:

[0159] The needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening, which can specifically include:

[0160] The needle opening of the sample needle 12 is kept in contact with the slide 21 to be coated, and the drive liquid module 11 is controlled to drive the sample to continuously output from the needle opening, which can specifically include:

[0161] Based on the above manner, since the needle for puncture and the needle for sample coating are different, the particulate matter generated by puncture is usually adhered to the puncture needle, and the possibility of adhering to the coating needle is low, so that when the sample liquid is coated based on the coating needle to form a sample coating layer, the possibility of the particulate matter falling on the slide 21 is small, thereby reducing the possibility of the particulate matter generated by puncture polluting the sample and negatively interfering with subsequent sample detection, and improving the reliability of the sample coating layer. In addition, since the output amount of the sample needs to be controlled during sample coating, the inner diameter of the needle for coating is small, that is, the overall rigidity is poor. If the sample test tube is directly punctured, the coating needle is easy to deform or even break, or the test tube cap is not punctured into the sample test tube. Therefore, the needle for puncture and the needle for sample coating are different, and the strength of the puncture needle is greater than that of the coating needle.

[0162] In an embodiment, during the continuous output of the sample from the needle opening, the needle opening is kept in contact with the slide, and the stage and the needle opening are controlled to move relatively in at least one first target direction to form a sample coating layer on the slide 21. After that, the control module is further used for:

[0163] When the projection of the slide 21 and the projection of the needle opening do not overlap in a second target direction D2, the control liquid module 11 is controlled to deliver a cleaning liquid to the sample needle 12 to clean the sample needle 12.

[0164] The second target direction D2 is perpendicular to the stage plane.

[0165] Specifically, after the sample coating layer is formed, the needle opening of the sample needle 12 is controlled to move relative to the slide 21, so that the projection of the needle opening of the sample needle 12 in the second target direction D2 and the projection of the slide 21 in the second target direction D2 do not overlap each other, that is, the projection of the needle opening of the sample needle 12 in the second target direction D2 and the projection of the slide 21 in the second target direction D2 are staggered. Thereafter, the control liquid module 11 is controlled to deliver a cleaning liquid to the sample needle 12 to clean the sample remaining in the sample needle 12. Based on this manner, even if the waste liquid generated by cleaning drops from the needle opening of the sample needle 12, without other factors, the dropped waste liquid is difficult to fall on the slide 21, that is, the possibility of the waste liquid generated by cleaning polluting the formed sample coating layer can be reduced, and the reliability of the sample coating layer is improved.

[0166] Optionally, the control liquid module 11 includes a first control liquid unit and a second control liquid unit.

[0167] The first control liquid unit is used to deliver a sample to the sample needle 12.

[0168] The second control liquid unit is used to deliver a cleaning liquid to the sample needle 12.

[0169] The first driving liquid unit transports the sample at a flow rate less than the flow rate at which the second driving liquid unit transports the cleaning liquid.

[0170] Specifically, the sample needle 12 can be connected to the first driving liquid unit and the second driving liquid unit, respectively, wherein the first driving liquid unit and the second driving liquid unit both have the ability to provide driving force to drive the corresponding liquid, the first driving liquid unit is used to transport the sample to the sample needle 12, so that the sample is continuously output to the smear 21 to form a sample coating, and the second driving liquid unit is used to transport the cleaning liquid to the sample needle 12, so that the cleaning liquid processes the residue of the sample in the sample needle 12 to achieve cleaning of the sample needle 12.

[0171] Since the amount of sample required to form a sample coating on the smear 21 is very small, generally less than 5ul, it needs to be accurate and stable to avoid the cell overlap of the sample coating being too high, which is not conducive to the formation of a single-layer cell sample coating; when the cleaning liquid is transported to clean the sample needle, the flow rate and liquid volume of the cleaning liquid need to be large to achieve better cleaning effect. Therefore, the flow rate of the first driving liquid unit transporting the sample can be less than the flow rate of the second driving liquid unit transporting the cleaning liquid, so that the controllability of the first driving liquid unit transporting the sample is stronger than that of the second driving liquid unit transporting the cleaning liquid, and the transportation intensity of the second driving liquid unit transporting the cleaning liquid is stronger than that of the first driving liquid unit transporting the sample.

[0172] In summary, based on the above method, the stability and accuracy of the first driving liquid unit transporting the sample to the sample needle 12 are improved, small flow output of the sample during coating can be realized to reduce the possibility of the sample coating having too high cell overlap, and the output speed of the cleaning liquid during cleaning can be increased to improve the cleaning efficiency.

[0173] In an embodiment, during the continuous output of the sample from the needle opening, the needle opening is kept in contact with the smear 21, and the relative movement of the stage 13 and the needle opening in at least one first target direction D1 is controlled, so that after the sample coating is formed on the smear 21, the control module is further used for:

[0174] First, the driving liquid module 11 is controlled to suck the sample liquid through the sample needle 12, and then the needle opening at the other end of the sample needle 12 is controlled to move away from the smear 21.

[0175] Specifically, after the sample is continuously output through the sample needle 12 and the generation of the sample coating on the smear 21 is completed, the driving liquid module 11 is first controlled to provide suction to the sample needle 12 to promote the sample needle 12 to suck the sample present in its inner cavity or at its needle head, and then the sample needle 12 is controlled to stop contacting and moving away from the smear 21.

[0176] Based on the above manner, after forming the sample coating by coating with the sample needle 12, the excess sample can be first sucked, and then the sample needle 12 is lifted to complete the preparation of the prepared smear 21 containing the sample coating, which can reduce the possibility that the excess sample attached to the sample needle 12 drops back to the sample coating due to the action of lifting the sample needle 12, thereby damaging the formed sample coating, and further improves the reliability of the sample coating. It can also avoid too much sample at the corresponding position of the sample coating, causing the cell overlap degree to be too high at this position, affecting the subsequent observation and detection of the sample coating, and improving the reliability of the sample coating.

[0177] In an embodiment, the control module is further configured to control the liquid output module 11 to output the sample to make the sample infiltrate the needle opening of the sample needle 12 before controlling the relative movement of the object table 13 and the needle opening in at least one first target direction D1.

[0178] Based on the above manner, first, by performing the above relative displacement in the first target direction D1 and continuously outputting the sample while the needle opening of the sample needle 12 is in contact with the prepared smear 21, the sample output from the sample needle 12 can directly reach the prepared smear 21 after leaving the sample needle 12, thereby reducing the occurrence of the situation that the sample impacts the prepared smear 21 due to gravity acceleration or too high sample output rate or too much sample output after the sample leaves the sample needle 12, further reducing the possibility of liquid splashing due to the impact of the prepared smear 21, allowing the sample to stably fall on the user's desired position on the slide, improving the controllability of sample output, and reducing the possibility of discontinuous sample coating or too high cell overlap degree in the sample coating due to liquid splashing.

[0179] Secondly, by making the sample infiltrate the needle opening of the sample needle 12 before the relative displacement of the needle opening of the sample needle 12 and the prepared smear 21 in the first target direction D1, that is, before the needle opening of the sample needle 12 contacts the prepared smear 21 or before the needle opening of the sample needle 12 contacts the prepared smear 21 but has not started the above relative displacement in the first target direction D1 and continuously outputting the sample, the contact surface between the needle opening of the sample needle 12 and the prepared smear 21 can be filled with sample after the needle opening of the sample needle 12 contacts the prepared smear 21, which can improve the continuity of the sample coating formed by the sample during the subsequent above relative displacement in the first target direction D1 and continuously outputting the sample, and can improve the uniformity of the sample coating, improve the controllability and rationality of the cell overlap degree.

[0180] In summary, the reliability of smear preparation can be effectively improved.

[0181] In the view of Figure 1, when the needle port of the sample needle 12 is always kept in contact with the to-be-smear 21 located on the object table 13, the second target direction D2 is perpendicular to the first target direction D1, the second target direction D2 can be upward or downward, and the first target direction D1 can be forward or backward, left or right, or any direction parallel to the side of the to-be-smear 21 on which the sample is carried, which is not limited here.

[0182] In the view of Figure 1, in the first example, the control module can be used to control the sample needle 12 to move up and down in the second target direction D2, and control the object table 13 to move forward and backward and left and right in the at least one first target direction D1.

[0183] In the second example, the control module can be used to control the sample needle 12 to move forward and backward and left and right in the at least one first target direction D1, and control the object table 13 to move up and down in the second target direction D2.

[0184] In the third example, the control module can be used to control the object table 13 to be stationary, and control the sample needle 12 to move up and down in the second target direction D2, and move forward and backward and left and right in the at least one first target direction D1.

[0185] In the fourth example, the control module can be used to control the sample needle 12 to be stationary, and control the object table 13 to move up and down in the second target direction D2, and move forward and backward and left and right in the at least one first target direction D1.

[0186] It can be understood that the up and down movement in the second target direction D2 is to make the sample needle 12 contact the to-be-smear 21, and the forward and backward and left and right movement in the first target direction D1 is to form the sample coating on the to-be-smear 21.

[0187] Optionally, the smear device further comprises a cleaning swab module 18.

[0188] The control module is further used to:

[0189] After the control liquid output module 11 outputs the sample to make the sample infiltrate the needle port of the sample needle 12, the control cleaning swab module 18 is used to absorb part of the sample located outside the sample needle 12 at the needle port of the sample needle 12.

[0190] Specifically, after the sample infiltrates the needle port of the sample needle 12, part of the sample located outside the sample needle 12 is absorbed to reduce the possibility of the occurrence of the situation that the overlapping degree of the cells located at or near the needle port of the sample needle 12 in the subsequent sample coating is too high due to too much liquid at the needle port of the sample needle 12, and improve the reliability of smear preparation.

[0191] Further, if the sample invades the needle opening of the sample needle 12 before the sample needle 12 contacts the to-be-smear 21, the sample forms a convex liquid surface at the needle opening of the sample needle 12.

[0192] Specifically, before the needle opening of the sample needle 12 is controlled to keep in contact with the to-be-smear 21 placed on the object table 13, if no pre-treatment is performed, the liquid surface formed by the sample in the sample needle 12 is a concave liquid surface Z1, and generally the entire concave liquid surface Z1 is in the sample needle 12. If the needle opening of the sample needle 12 is directly controlled to contact the to-be-smear 21 at this time and the sample is output, air between the sample needle 12 and the to-be-smear 21 is easy to cause negative effects on the continuous output of the sample, reduce the output stability, and increase the possibility of discontinuous sample coating or too high cell overlapping in the subsequent sample coating. Therefore, before the needle opening of the sample needle 12 is controlled to keep in contact with the to-be-smear 21 placed on the object table 13, the sample can first form a convex liquid surface Z3 outside the sample needle 12 as shown in FIG. 10, the distance between the farthest end of the convex liquid surface Z3 and the second end of the sample needle 12 is T2, and then the cleaning swab module 18 is controlled to move to the needle opening of the sample needle 12 as shown in FIG. 9, so that the cleaning swab module 18 absorbs part of the sample outside the sample needle 12, but cannot completely absorb it, and still needs to absorb the convex liquid surface Z2 of the sample formed after the absorption, at least part of which is outside the sample needle 12, and the distance between the farthest end of the convex liquid surface Z2 and the second end of the sample needle 12 is T1, wherein T1 is less than T2.

[0193] Further, in other embodiments, the liquid driving module 11 can also be controlled to output the sample and maintain for a preset time after the needle opening of the sample needle 12 is controlled to keep in contact with the to-be-smear 21 placed on the object table 13, so that the sample invades the needle opening of the liquid ejector. In this way, the air between the contact surface of the sample needle 12 and the to-be-smear 21 can also be discharged by the blood sample, so that when the relative displacement in the first target direction D1 is performed, the sample continuously output from the sample needle 12 can be as little as possible to be negatively interfered by the air, and can be stably output, reducing the possibility of discontinuous sample coating or too high cell overlapping in the sample coating, and improving the reliability of the smear preparation.

[0194] Based on the above manner, the second end of the sample needle 12 has a certain amount of sample outside the sample needle 12, so that when the second end of the sample needle 12 is in contact with the to-be-smear 21, due to the presence of the blood sample outside the sample needle 12, there is only a small amount of air or no air between the contact surface of the sample needle 12 and the to-be-smear 21, so that when the relative displacement in the first target direction D1 is performed, the sample continuously output from the sample needle 12 can be as little as possible to be negatively interfered by the air, and can be stably output, reducing the possibility of discontinuous sample coating or high cell overlapping degree in the sample coating, and improving the reliability of smear preparation.

[0195] In addition, by using the cleaning swab module 18 to absorb part of the sample outside the sample needle 12 after forming the initial convex liquid surface Z3, the liquid surface of the sample changes from the convex liquid surface Z3 to the convex liquid surface Z2, reducing the possibility of sample dripping due to the excessive area of the liquid surface of the sample or too much sample outside the sample needle 12, and further reducing the possibility of sample liquid splashing or device pollution caused by sample droplets, and reducing the possibility of discontinuous sample coating or high cell overlapping degree in the sample coating caused by blood sample splashing, further improving the reliability of smear preparation.

[0196] Optionally, before the needle port of the sample needle 12 and the to-be-smear 21 are relatively displaced in the first target direction D1, the liquid driving module 11 is controlled to output the sample to make the sample infiltrate the needle port of the sample needle 12, which can specifically include:

[0197] Before the needle port of the sample needle 12 and the to-be-smear 21 are in contact, the liquid driving module 11 is controlled to output the sample to make the sample infiltrate the needle port of the sample needle 12 and form a convex liquid surface outside the sample needle 12.

[0198] Specifically, based on the above manner, before the needle port of the sample needle 12 and the to-be-smear 21 are in contact, the second end of the sample needle 12 has a certain amount of sample outside the sample needle 12, so that after the second end of the sample needle 12 is in contact with the to-be-smear 21, due to the presence of the blood sample outside the sample needle 12, there is only a small amount of air or no air between the contact surface of the sample needle 12 and the to-be-smear 21, so that when the relative displacement in the first target direction D1 is performed, the sample continuously output from the sample needle 12 can be as little as possible to be negatively interfered by the air, and can be stably output, reducing the possibility of discontinuous sample coating or high cell overlapping degree in the sample coating, and improving the reliability of smear preparation.

[0199] Further, before the needle opening of the sample needle 12 contacts the to-be-smear 21, the control liquid driving module 11 outputs the sample so that the sample invades the needle opening of the sample needle 12 and forms a convex liquid surface outside the sample needle 12, which can specifically include:

[0200] Before the needle opening of the sample needle 12 contacts the to-be-smear 21, and when the to-be-smear 21 and the needle opening of the sample needle 12 are misaligned in a second target direction D2, the control liquid driving module 11 outputs the sample so that the sample invades the needle opening of the sample needle 12 and forms a convex liquid surface outside the sample needle 12.

[0201] The second target direction D2 is perpendicular to the first target direction D1, or the second target direction D2 is perpendicular to the object plane.

[0202] Specifically, assuming that the first target direction D1 is a horizontal direction, the second target direction D2 is a vertical direction or a gravity direction. By misaligning the to-be-smear 21 and the sample needle 12 in the second target direction D2, when the convex liquid surface is formed and the sample is too much to drip, the sample that drips will not drip onto the to-be-smear 21 along the second target direction D2, thereby reducing the possibility that the sample formed on the to-be-smear 21 is discontinuous or the cell overlapping degree is too high, and further improving the reliability of smear preparation.

[0203] In an embodiment, the ratio of the length of the sample coating in the length direction of the to-be-smear 21 to the length of the to-be-smear 21 is greater than 1 / 2.

[0204] Specifically, by making the ratio of the length of the sample coating in the length direction of the to-be-smear 21 to the length of the to-be-smear 21 greater than 1 / 2, a coating area with a large proportion can be divided on the to-be-smear 21, which is used for the sample liquid to form a sample coating. The greater the proportion of the coating area in the to-be-smear 21, the more difficult it is to control the needle opening of the sample needle 12 to move in the coating area and continuously output the sample liquid to form a sample coating, and the fault tolerance rate of the sample coating can be improved, and the possibility of the sample coating being discontinuous or the cell overlapping degree being too high can be further reduced.

[0205] In an embodiment, after controlling the object table 13 and the needle opening to move relatively in at least one first target direction D1 to form a sample coating on the to-be-smear 21, the control module is further configured to:

[0206] First, control the liquid driving module 11 to suck the sample through the sample needle 12, and then control the needle opening of the sample needle 12 to move away from the to-be-smear 21.

[0207] Specifically, after the sample is continuously output through the sample needle 12 and the generation of the sample coating on the to-be-smear 21 is completed, the liquid driving module 11 is first controlled to provide suction to the sample needle 12 to cause the sample needle 12 to suck the sample present in the inner cavity or at the needle port of the sample needle 12, and then the sample needle 12 is controlled to stop being in contact with and moving away from the to-be-smear 21.

[0208] Based on the above manner, after the sample coating is formed by the sample needle 12, the excess sample can be first sucked and then the sample needle 12 is lifted to complete the preparation of the to-be-smear 21 containing the sample coating, which can reduce the possibility that the excess sample attached to the sample needle 12 drops back to the sample coating due to the action of lifting the sample needle 12, thereby damaging the formed sample coating, further improve the reliability of the sample coating, and meanwhile, the excess sample between the sample needle 12 and the to-be-smear 21 is sucked away by the liquid driving module 11, avoiding too much sample at the corresponding position of the sample coating, causing the cell overlapping degree to be too high at this position, affecting the subsequent observation and detection of the sample coating, and improving the reliability of the sample coating.

[0209] Optionally, the liquid driving module 11 includes a first liquid driving unit and a second liquid driving unit.

[0210] The first liquid driving unit is configured to deliver the sample to the sample needle 12.

[0211] The second liquid driving unit is configured to suck the sample through the sample needle 12.

[0212] The flow rate at which the first liquid driving unit delivers the sample is less than the flow rate at which the second liquid driving unit sucks the sample.

[0213] Specifically, the first liquid driving unit is mainly configured to provide a pushing force to the sample needle 12 and deliver the sample, so that the sample is output from the liquid outlet end of the sample needle 12 to form a sample coating.

[0214] The second liquid driving unit is mainly configured to provide a suction to the sample needle 12 and suck the sample, so that the sample is collected through the sample needle 12, or the excess sample is sucked back before the sample needle 12 is lifted after the sample coating is formed.

[0215] Since the amount of sample required for the sample coating is very small, generally less than 5ul, by making the flow rate at which the first liquid driving unit delivers the sample less than the flow rate at which the second liquid driving unit sucks the sample, the controllability of the first liquid driving unit in continuously outputting the sample to form the sample coating can be improved, avoiding the phenomenon of sample spatter and serious cell overlapping caused by too much output of the sample, thereby reducing the possibility of discontinuity or too high cell overlapping degree of the sample coating, further improving the reliability of the sample coating, and meanwhile, the second liquid driving unit with a larger flow rate is used for sample collection or sucking back the excess sample before the sample needle 12 is lifted after the sample coating is formed, which can shorten the time for sample collection and sucking back of the excess sample, improving the efficiency.

[0216] It should be noted that the first driving liquid unit and the second driving liquid unit mentioned in the foregoing embodiments are driving liquid units of the same type, and the range of the first driving liquid unit is smaller than that of the second driving liquid unit.

[0217] Alternatively, the first driving liquid unit and the second driving liquid unit mentioned in the foregoing embodiments are driving liquid units of different types, and the range of the first driving liquid unit is smaller than that of the second driving liquid unit.

[0218] Specifically, when the first driving liquid unit and the second driving liquid unit are driving liquid units of the same type, the first driving liquid unit and the second driving liquid unit can both be syringes, or can be driving liquid units of other types, which are not limited here.

[0219] When the first driving liquid unit and the second driving liquid unit are driving liquid units of different types, the first driving liquid unit can be a syringe, and the second driving liquid unit can be a quantitative pump, or can be a combination of driving liquid units of other types, which are not limited here.

[0220] Based on the above manner, by making the range of the first driving liquid unit smaller than that of the second driving liquid unit when the first driving liquid unit and the second driving liquid unit are driving liquid units of the same type, the stability and precision of the first driving liquid unit in delivering the sample to the sample needle 12 can be improved. Moreover, by making the first driving liquid unit a unit with higher stability and precision in delivering the sample relative to the second driving liquid unit when the first driving liquid unit and the second driving liquid unit are driving liquid units of different types and the range of the first driving liquid unit is smaller than that of the second driving liquid unit, the stability and precision of the first driving liquid unit in delivering the sample to the sample needle 12 can also be improved, thereby improving the reliability of the sample coating.

[0221] The present application also proposes a smearing method applied to the smearing device of any one of the foregoing embodiments.

[0222] The smearing method comprises the following steps.

[0223] The needle opening of the sample needle 12 is controlled to be in contact with the to-be-smearing object 21, and the driving liquid module 11 is controlled to continuously output the sample from the needle opening.

[0224] During the continuous output of the sample from the needle opening, the needle opening is kept in contact with the to-be-smearing object 21, and the stage 13 and the needle opening are controlled to move relatively in at least one first target direction D1 to form a sample coating on the to-be-smearing object 21.

[0225] Specifically, the smearing method can further include the steps performed by the control module of any one of the foregoing embodiments, which are not described here again.

[0226] Differing from the prior art, in the technical solution of the application, the liquid driving module is used to deliver the sample to the sample needle to output from the needle port of the sample needle, the object table is used to carry the to-be-smear, and the control module is used to keep the needle port of the sample needle in contact with the to-be-smear during continuous output of the sample from the needle port of the sample needle, and control the object table and the needle port of the sample needle to relatively move in at least one first target direction, so as to realize smearing of the sample on the to-be-smear to form a sample coating on the to-be-smear. Based on the above manner, firstly, the sample can be smeared on any position on the sample smear during the relative movement, so as to form a uniform coating of any shape required by a user on the to-be-smear, and reduce the possibility that the coating formed on the to-be-smear cannot meet the sample detection requirement due to the over-thick coating and the over-high cell overlapping degree of a partial section, and / or due to the sample scarcity and the discontinuous coating of a partial section. Secondly, by keeping the needle port of the sample needle in contact with the to-be-smear for smearing, the possibility of sample liquid spatter can be reduced, the distance between the needle port of the sample needle and the to-be-smear does not need to be controlled, the sample coating formed is uniform and smooth, and the sample coating is regular and uninterrupted. In summary, the reliability of the sample smear is improved.

[0227] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, different embodiments or examples described in the specification and the features of different embodiments or examples can be combined and combined by those skilled in the art without contradiction.

[0228] In addition, the terms "first", "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0229] Any processes or methods described in the flowcharts or otherwise described herein can be understood as representing modules, segments, or portions of code that include one or more executable instructions for implementing specific logic functions or steps, and alternate implementations are possible. The preferred embodiments of the application should not be construed as limited to the order in which the steps are illustrated or discussed, and alternate implementations can be implemented in which the steps are performed in different orders, substantially simultaneously, or omitted entirely, depending on the functionality involved.

[0230] Logic and / or steps represented in the flowcharts or otherwise described herein, for example, can be embodied in computer-readable medium, which can be any device or apparatus that can store, communicate, propagate, or transport programming for use by or in connection with an instruction execution system, apparatus, or device. The computer-readable medium can be, for example, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium include the following: an electrical connection having one or more wires (electronic devices), a portable computer diskette (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or Flash memory), an optical fiber (optical device), and a portable compact disc read-only memory (CDROM). Note that the computer-readable medium can even be paper or another suitable medium upon which the program is printed, as the program can be electronically captured, for example via an optical scanner, then compiled, interpreted, or otherwise processed in a suitable manner, if necessary, and stored in a computer memory.

[0231] The above description is merely illustrative of the application, and does not limit the scope of the application, which is defined by the appended claims. Any equivalent structure or process variations, which transform the disclosed application into a technically equivalent application, are also included within the scope of the application.

Claims

1. A smearing device, characterized in that, The smearing device comprises: a driving liquid module; a sample needle, one end of which is connected to the driving liquid module, and the driving liquid module is configured to drive sample in the sample needle and / or a pipeline to output to a needle opening at the other end of the sample needle; a carrier table configured to carry a smear to be smeared with the sample; a control module configured to: control the needle opening of the sample needle to contact the smear to be smeared, and control the driving liquid module to drive the sample to output from the needle opening; during the process of the sample output from the needle opening, keep the needle opening in contact with the smear to be smeared, and control the carrier table to relatively move with the needle opening in at least one first target direction to form a sample coating on the smear to be smeared; wherein the first target direction is parallel to a carrier plane, and the carrier plane is a side of the carrier table facing the smear to be smeared.

2. The smear device of claim 1, wherein, The smearing device further comprises: a first moving module connected to the control module; a first elastic member connected to the sample needle and the first moving module respectively, and the first moving module is configured to drive the sample needle to move in a second target direction; wherein the second target direction is perpendicular to the carrier plane, and the first elastic member is configured to apply an elastic pushing force or an elastic pulling force to the sample needle in the second target direction.

3. Smear device according to claim 1 or 2, characterized in that The smearing device further comprises: a second moving module connected to the control module, and the carrier table is connected to the second moving module, and the second moving module is configured to control the carrier table to move in at least one first target direction.

4. The smearing device according to claim 1 or 2, characterized in that The smearing device further comprises: a second elastic member connected to the carrier table and the smear to be smeared respectively, and the second elastic member is configured to apply an elastic pushing force or an elastic pulling force to the carrier table in a second target direction; wherein the second target direction is perpendicular to the carrier plane.

5. The smearing device according to claim 1 or 2, characterized in that The other end of the sample needle is provided with at least one groove; the groove extends in the needle body of the sample needle from the end face of the other end of the sample needle in the direction of one end of the sample needle, and the groove is in communication with the needle opening of the sample needle.

6. The smear device of claim 5, wherein, The groove width of the groove on the end face of the other end of the sample needle is not less than 2 microns and not more than 100 microns, and / or the groove depth of the groove in the second target direction is not less than 2 microns and not more than 80 microns.

7. The smear device according to claim 1 or 2, characterized in that The control module is further configured to control the driving liquid module to output the sample to the needle opening before the needle opening of the sample needle contacts the smear to be smeared, and to form a convex liquid surface with at least part of the sample outside the needle opening; or, the control module is further configured to control the driving liquid module to output the sample to the needle opening before the carrier table relatively moves with the needle opening in at least one first target direction, and to make at least part of the sample contact the smear to be smeared.

8. The smearing device according to claim 1 or 2, characterized in that The first target direction comprises a first sub-target direction and a second sub-target direction, and the first sub-target direction is perpendicular to the second sub-target direction. The control of the relative movement of the objective table and the needle mouth in at least one first target direction comprises: controlling the relative movement of the objective table and the needle mouth in at least one first target direction, so that the sample needle alternately performs a first relative motion and a second relative motion relative to the objective table; wherein the first relative motion is that the sample needle moves a first preset distance in a first sub-target direction relative to the objective table, and then the sample needle moves a second preset distance in a second sub-target direction relative to the objective table; the second relative motion is that the sample needle moves a first preset distance in the opposite direction of the first sub-target direction relative to the objective table, and then the sample needle moves a second preset distance in the second sub-target direction relative to the objective table.

9. The smear device of claim 8, wherein, The second preset distance is the sum of the needle mouth diameter of the sample needle and a preset diffusion distance.

10. The smear device of claim 1 or 2, wherein, When controlling the relative movement of the objective table and the needle mouth in at least one first target direction, the speed of the relative movement is recorded as the relative movement speed; The preset expected thickness of the sample on the to-be-smear is recorded as the coating thickness; The average diffusion speed of the sample on the to-be-smear is recorded as the sample diffusion speed; The total time length of the diffusion of the sample on the to-be-smear is recorded as the sample diffusion time length; The maximum outer diameter of the sample needle in the first target direction is recorded as the sample needle outer diameter; The maximum inner diameter of the sample needle in the first target direction is recorded as the sample needle inner diameter; The speed of the sample needle conveying the sample is recorded as the sample conveying speed; The sample conveying speed is not less than a first speed and not greater than a second speed; wherein the first speed is the product of the relative movement speed, the coating thickness and a first value, the second speed is the product of the relative movement speed, the coating thickness and a second value, the first value is the sum of a third value and the sample needle inner diameter, the second value is the sum of the third value and the sample needle outer diameter, and the third value is the product of the sample diffusion speed and the sample diffusion time length.

11. The smear device of claim 1 or 2, wherein, During the output of the sample from the needle mouth, the needle mouth is kept in contact with the to-be-smear, and the control of the relative movement of the objective table and the needle mouth in at least one first target direction is performed, so that after forming a sample coating on the to-be-smear, the control module is further used for: controlling the projection of the to-be-smear and the projection of the needle mouth not to overlap in a second target direction, and then controlling the liquid driving module to convey cleaning liquid to the sample needle to clean the sample needle.

12. The smear device of claim 11, wherein, The liquid driving module comprises: a first liquid driving unit, which is used to convey the sample to the sample needle; a second liquid driving unit, which is used to convey cleaning liquid to the sample needle; wherein the flow rate of the first liquid driving unit conveying the sample is less than the flow rate of the second liquid driving unit conveying the cleaning liquid.

13. The smear device according to claim 1 or 2, wherein the control module is further used for: Before controlling the stage and the needle port to move relatively in at least one first target direction, the control module controls the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle.

14. The smear device of claim 13, wherein, The smearing device further comprises a cleaning swab module; The control module is further configured to: After controlling the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle, the control module controls the cleaning swab module to absorb part of the sample outside the sample needle at the needle port of the sample needle.

15. The smear device of claim 13, wherein, The control of the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle before the relative displacement of the needle port of the sample needle and the object to be smeared in the first target direction comprises: After the needle port of the sample needle contacts the object to be smeared, the control of the liquid driving module to output the sample and maintain for a preset time length so that the sample infiltrates the needle port of the sample needle.

16. The smear device of claim 13, wherein, The control of the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle before the relative displacement of the needle port of the sample needle and the object to be smeared in the first target direction comprises: Before the needle port of the sample needle contacts the object to be smeared, the control of the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle and forms a convex liquid surface outside the sample needle.

17. The smear device of claim 16, wherein, The control of the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle before the needle port of the sample needle contacts the object to be smeared comprises: Before the needle port of the sample needle contacts the object to be smeared, and when the object to be smeared and the needle port of the sample needle are misaligned in a second target direction, the control of the liquid driving module to output the sample so that the sample infiltrates the needle port of the sample needle and forms a convex liquid surface outside the sample needle; The second target direction is perpendicular to the object plane.

18. The smear device of claim 1 or 2, wherein, The ratio of the length of the sample coating in the length direction of the object to be smeared to the length of the object to be smeared is greater than 1 / 2.

19. The smear device of claim 1 or 2, wherein, After the control of the stage and the needle port to move relatively in at least one first target direction to form a sample coating on the object to be smeared, the control module is further configured to: First, control the liquid driving module to suck the sample into the sample needle, and then control the needle port of the sample needle to move away from the object to be smeared.

20. The smear device of claim 19, wherein, The liquid driving module comprises: A first liquid driving unit, configured to deliver the sample to the sample needle; A second liquid driving unit, configured to suck the sample into the sample needle; The flow rate of the sample delivered by the first liquid driving unit is less than the flow rate of the sample sucked into the sample needle by the second liquid driving unit.

21. A method of smearing, characterized by The smearing device is applied to any one of claims 1 to 18; The smearing method comprises: Controlling the needle port of the sample needle to contact the object to be smeared, and controlling the liquid driving module to drive the sample to be output from the needle port; During the outputting of the sample from the needle port, the needle port is kept in contact with the object to be smeared, and the relative movement of the object table and the needle port in at least one first target direction is controlled to form a sample coating on the object to be smeared.

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