Antibacterial structure comprising antibacterial polymeric layer and polymeric support layer carrying antibacterial component, and preparation method thereof

Abstract

The present invention provides an antibacterial structure comprising: a polymeric support layer carrying an antibacterial component; and an antibacterial polymeric layer applied onto the support layer. The antibacterial structure according to the present invention exhibits an excellent antibacterial effect, shows a contact angle of 150° or higher with a solvent even if the antibacterial component is carried, and has antifouling properties.

Description

Antibacterial structure comprising an antibacterial polymer layer and a polymer support layer containing an antibacterial component, and a method for manufacturing the same

[0001] The present invention relates to an antibacterial structure having excellent antibacterial properties by coating an antibacterial polymer on a polymer support layer containing an antibacterial component.

[0002]

[0003] As hygienic living becomes more actively demanded in daily life, antibacterial agents are being used in various fields.

[0004] In a prior invention, an antibacterial polymer was produced by graft polymerizing a VDF polymer with a quaternary ammonium-containing acrylic monomer and a fluorinated acrylic monomer, thereby realizing excellent antibacterial properties, good solubility in general-purpose organic solvents, and superhydrophobic / superoleophobic properties (Korean Patent Publication No. 10-2023-0153627). However, the antibacterial polymer coating alone has the disadvantage of making it difficult to use for a long period of time.

[0005] Accordingly, the inventors of the present invention developed a structure having enhanced antibacterial properties by coating an antibacterial polymer on a polymer support layer to ensure durability and additionally loading an antibacterial component into the polymer support layer.

[0006]

[0007] An object of one aspect of the present invention is to provide an antibacterial structure having excellent antibacterial properties, particularly the ability to kill bacteria, by containing an antibacterial component therein.

[0008] In addition, another object of the present invention is to provide a method for producing the antimicrobial structure.

[0009] In addition, another object of the present invention is to provide an antibacterial substrate including an antibacterial structure in which a polymer support layer is laminated on an adhesive layer.

[0010] In addition, another object of the present invention is to provide a method of using the antibacterial substrate.

[0011]

[0012] In the present invention,

[0013] A polymer support layer containing an antibacterial ingredient; and

[0014] An antimicrobial structure is provided, comprising an antimicrobial polymer layer coated on the support layer.

[0015]

[0016] The structure according to the present invention exhibits an excellent antibacterial effect, exhibits a contact angle of 150° or more with respect to a solvent despite containing an antibacterial component, and has antifouling properties.

[0017]

[0018] Figure 1 is a schematic diagram showing the manufacturing of a polymer support layer loaded with an antibacterial component.

[0019] Figure 2 is a schematic diagram showing spray coating of an antibacterial polymer on a polymer support layer.

[0020] Figure 3 is a schematic diagram showing an overall method for manufacturing a structure of the present invention.

[0021] Fig. 4 is an actual photograph of the structure of the present invention.

[0022] Figure 5 is a photograph of the surface of the structure after spray coating taken with an optical microscope at 200x magnification.

[0023] Figure 6 is a photograph of the surface of the structure after spray coating taken with an optical microscope at 500x magnification.

[0024] Figure 7 is a photograph taken at 1,000x magnification of the surface using a scanning electron microscope.

[0025] Figure 8 is a photograph taken at 10,000x magnification of the surface using a scanning electron microscope.

[0026] Figure 9a is a photograph showing the contact angle for water on a surface spray-coated on a polymer support layer that does not contain oil (antibacterial component).

[0027] Figure 9b is a photograph showing the contact angle for water on a spray-coated surface after plasma treatment to improve polymer adhesion to a polymer support layer.

[0028] Figure 9c is a photograph showing the contact angle of water on a surface spray-coated on a polymer support layer containing oil (antibacterial component).

[0029] Fig. 10 is a photograph showing an appearance of oil being released from a structure through finger contact. Fig. 10a is a photograph of the structure of the present invention, Fig. 10b is a photograph showing a finger being brought into contact with the structure, Fig. 10c shows an antibacterial component left on a finger after contact, and Fig. 10d shows an appearance of an antibacterial component being released through the structure.

[0030] Figure 11a is a photograph showing the contact angle of the structure film with water before the 85 / 85 test.

[0031] Figures 11b and 11c are photographs showing the contact angle of the structure film with water after performing the 85 / 85 test under the same conditions, respectively.

[0032]

[0033] Hereinafter, the present invention will be described in detail.

[0034] The embodiments of the present invention may be modified in various ways, and the scope of the present invention is not limited to the embodiments described below. Furthermore, the embodiments of the present invention are provided to more fully explain the present invention to those of ordinary skill in the art.

[0035] Furthermore, reference to an element "including" throughout the specification does not exclude other elements, but rather includes other elements, unless specifically stated otherwise.

[0036]

[0037] One aspect of the present invention is:

[0038] A polymer support layer containing an antibacterial ingredient; and

[0039] An antimicrobial structure is provided, comprising an antimicrobial polymer layer coated on the support layer.

[0040] Antimicrobial ingredients are substances that inhibit the growth of microorganisms or have a bactericidal effect. Examples of substances containing antimicrobial ingredients include plant-based essential oils. Plant-based essential oils are volatile plant concentrates extracted from plants, typically refined aromatic oils or gasoline extracted from flowers, leaves, or fruits.

[0041] In this specification, plant-based essential oils include, but are not limited to, essential oils extracted from plants containing antibacterial ingredients, and the plants include various plants such as rosemary, lemongrass, bergamot, sage, hinoki, tea tree, eucalyptus, pine, mountain ash, yuzu, and angelica.

[0042] The content of the antibacterial component for the entire polymer support layer is not particularly limited, but can be used in amounts of 5 wt% or more, 10 wt% or more, 20 wt% or more, 30 wt% or more, 40 wt% or more, or 50 wt% or more. However, in terms of the structural stability of the polymer support layer, it can be used in amounts of 60 wt% or less or 50 wt% or less.

[0043] The above structural support polymer may include a polysiloxane-based, polyester-based, polyamide-based, polyurethane-based, or poly(meth)acrylate-based polymer, and in one example of the present invention, PDMS was used.

[0044] The above polymer support layer may further include a release rate control agent to control the release rate of the antibacterial component.

[0045] Release rate modifiers may include oils that are non-toxic to humans and do not evaporate easily, such as paraffin, shea butter, coconut oil, jojoba oil, and beeswax.

[0046] Paraffin is, for example, a straight-chain hydrocarbon having 20 to 40 carbon atoms, or 20 to 35 carbon atoms, and may be a single type or a mixture of several types of hydrocarbons in terms of carbon length, and is generally provided as a mixture of hydrocarbons having different carbon atoms. Paraffin may contain impurities other than the hydrocarbons having the aforementioned carbon atoms in amounts of 20% or less, 10% or less, 5% or less, or 1% or less. Products provided under the trademark Vaseline also contain petrolatum obtained during the crude oil refining process, which is a mixture containing paraffin. Paraffin included in the polymer support layer can control the release rate of the antimicrobial component.

[0047] The release rate modifier may be included in an amount of 1 wt% to 50 wt%, 5 wt% to 40 wt%, 10 wt% to 30 wt%, or 15 wt% to 25 wt% relative to the entire polymer support layer.

[0048] In order to ensure the structural stability of the polymer support layer, the structural support polymer can be used in an amount of 30 wt% or more, 40 wt% or more, or 50 wt% or more relative to the entire polymer support layer.

[0049] In the present invention, the antibacterial polymer layer includes an antibacterial polymer, and the antibacterial polymer may include an antibacterial fluorine-based polymer.

[0050] The above antibacterial fluorinated polymer may include a repeating unit derived from a CTFE (chlorotrifluoroethylene) monomer, and may be a copolymer further including a repeating unit derived from a fluorinated monomer such as VDF (vinylidene fluoride), TFE (tetrafluoroethylene), trifluoroethylene, HFP (hexafluoropropylene), TrFE (trifluoroethylene), HFIB (hexafluoroisobutylene), or perfluoroalkyl vinyl ether.

[0051] The above copolymer may be a random, alternating, or block copolymer, and is preferably a random copolymer.

[0052] As an example, the antibacterial polymer layer may include a polymer in which a vinyl monomer containing a quaternary ammonium ion is grafted onto the fluorinated polymer backbone. Optionally, the antibacterial polymer layer may further graft a fluorinated (meth)acrylic monomer onto the fluorinated polymer backbone.

[0053] As an example,

[0054] The vinyl monomer containing a quaternary ammonium ion in the polymer main chain may be a compound represented by the following chemical formula 1.

[0055] [Chemical Formula 1]

[0056]

[0057]

[0058] In the above chemical formula 1,

[0059] R1 to R3 are each independently C- 1-4 It is alkyl;

[0060] X is Cl - , Br - , I - , AlCl4 - , Al2Cl7 - , BF4 - , PF6 - , ClO4 - , NO3 - , CH3COO - , CF3COO - , CH3SO3 - , CF3SO3 - , (CF3SO2)2N - , (CF3SO2)3C - ,AsF6 - , SbF6 - , NbF6 - , TaF6 - , (CN)2N - , C4F9SO3 - , (C2F5SO2)2N - , C3F7COO - or (CF3SO2)(CF3CO)N -and X is H or CH3, Y- is any anion, and n is 1 to 6.

[0061] The above fluorinated (meth)acrylic monomer may be selected from the group consisting of trifluoroethyl methacrylate, tetrafluoropropyl methacrylate, trifluoroethyl methacrylate, pentafluoropropyl methacrylate, octafluoropentyl methacrylate, tetrafluoroethyl methacrylate, hexafluorobutyl methacrylate, and heptafluorobutyl methacrylate, and preferably pentafluoropropyl methacrylate (PFPMA).

[0062] The vinyl monomer and the fluorine-based (meth)acrylic monomer containing the above-mentioned quaternary ammonium ion can be introduced sequentially or simultaneously to be grafted, and the resulting grafted branch chain can be composed of a single component or can be composed of two or more types.

[0063] The above antibacterial polymer may have a molecular weight of 1,000 to 999,000, 100,000 to 900,000, 150,000 to 800,000, 250,000 to 700,000, 300,000 to 500,000, or 300,000 to 400,000.

[0064] As a specific example, the above antibacterial polymer may be a copolymer in which PFPMA (pentafluoropropyl methacrylate) and QDMA (2-(methacryloyloxyethyl)trimethylammonium iodide) are grafted onto the P(VDF-CTFE) polymer main chain.

[0065] In the antibacterial structure of the present invention, an empty space exists within the polymer support layer, allowing an antibacterial component to be loaded therein, and the antibacterial polymer layer is formed by coating an antibacterial polymer on the polymer support layer to form a three-dimensional network in the form of fibers. Accordingly, the antibacterial component can be released outside the structure through the polymer support layer and the antibacterial polymer layer.

[0066]

[0067] Another aspect of the present invention is:

[0068] A step of forming a polymer support layer containing an antibacterial ingredient;

[0069] Comprising a step of coating an antibacterial polymer layer on a support layer,

[0070] A method for manufacturing the above-described antibacterial structure is provided.

[0071] In the step of forming a polymer support layer, the polymer support layer is manufactured by polymerizing a polymer-forming monomer so as to include a crosslinking agent and an antibacterial component.

[0072] Specifically, PDMS monomer and crosslinker are added to the mixing container in an amount of 20 wt% to 90 wt%, 30 wt% to 85 wt%, or 40 wt% to 80 wt% based on the total mixture weight.

[0073] Antibacterial component in an amount of 1 wt% to 50 wt%, 5 wt% to 40 wt%, 10 wt% to 30 wt% or 15 wt% to 25 wt% and

[0074] A release rate control agent is added in an amount of 1 wt% to 50 wt%, 5 wt% to 40 wt%, 10 wt% to 30 wt%, or 15 wt% to 25 wt% and mixed. The mixture is then placed in a mold and cured to produce a polymer support layer loaded with an antibacterial component.

[0075] In the step of coating the antimicrobial polymer layer, the antimicrobial polymer is coated on the support layer. Any method capable of coating a substrate can be used without particular restrictions. However, spin coating, dip coating, roll coating, solution coating, and spray coating are preferred, with spray coating being more preferred. This allows the antimicrobial polymer to form a three-dimensional network in the form of fibers.

[0076] The spray coating technique described above involves dissolving or dispersing a solid component in a solvent and spraying it onto a substrate through a nozzle with compressed gas. This technique is widely used in industry. The sprayed solution is ejected from the nozzle in the form of fine particles. The solvent evaporates upon or after reaching the substrate, leaving the solid component behind. The fine particles exhibit a significantly increased surface area compared to when they are agglomerated. If the solvent is a low-boiling-point solvent with rapid evaporation, it will evaporate instantly before reaching the substrate.

[0077]

[0078] Another aspect of the present invention is:

[0079] Deposition film layer;

[0080] an adhesive layer formed on a release film; and

[0081] An antibacterial substrate is provided, comprising the above-described antibacterial structure in which a polymer support layer is laminated on an adhesive layer.

[0082] A release film refers to a film that has a release property that does not adhere well to an adhesive component, and can play a role in protecting the surface of a target object, the surface of an adhesive, the surface of an adhesive, etc. The release film can be manufactured and configured in the form of a thin film coating a coating composition on a substrate (PET, PE, PP, paper, etc.).

[0083] Antibacterial materials can be used by adhering to a desired object due to the presence of an adhesive layer. The adhesive layer contains an adhesive, which exerts a certain degree of adhesive strength and / or adhesion to bond or adhere various adherends to each other. The adhesive may be any material with adhesive strength, including, but not limited to, epoxy resin, acrylic resin, urethane resin, and silicone resin.

[0084]

[0085] Another aspect of the present invention is:

[0086] A step of removing a release film layer of an antibacterial substrate;

[0087] A method of using an antibacterial substrate is provided, comprising a step of adhering an antibacterial substrate from which a heteromorphic film layer has been removed to a surface of a target object.

[0088] By removing the release film layer of the antibacterial substrate, the adhesive layer of the antibacterial substrate can come into contact with and adhere to the target object.

[0089]

[0090] Hereinafter, the present invention will be described in detail through examples.

[0091] However, the examples described below are only specific examples of one aspect of the present invention, and the present invention is not limited thereto.

[0092]

[0093] Manufacturing Example 1. Manufacturing of antibacterial polymer composition (PVDF-PFPMA-QDMA)

[0094] Step 1: A solution was prepared with 30 g of a random copolymer of vinylidene fluoride (VDF) and chlorotrifluoroethylene (CTFE) (molecular weight 1,000 to 999,000), 500 g of dimethyl sulfoxide, 30 g of a fluorinated methacrylic monomer PFPMA (pentafluoropropyl methacrylate), 20 g of a cationic monomer QDMA (2-(methacryloyloxyethyl)trimethylammonium iodide), 1 g of monovalent copper chloride, and 2.0 ml of pentamethyldiethylenetriamine.

[0095] Step 2: Afterwards, the solution was stirred at a temperature of 95°C and a stirring speed of 500 rpm to perform polymerization.

[0096]

[0097] Manufacturing Example 2. Antibacterial ingredient (antibacterial oil)

[0098] Among the known antibacterial oils, candidates were selected, and the minimum inhibitory concentration (MIC) of Escherichia coli (E. coli) was measured to evaluate antibacterial activity, which is summarized in Table 1 below.

[0099] Oil E. Coli Minimum Inhibitory Concentration (mg ml -1 )Rosemary 4.5~10, lemongrass 0.6, bergamot 0.5, sage 3.5-5, hinoki 12.5, tea tree 2.5, eucalyptus 0.09

[0100] Additionally, the evaporation rates of eucalyptus, hinoki, and tea tree among antibacterial oils were evaluated and shown in Table 2.

[0101] Oil Residual Amount (%) 0 hours 6 hours 1 day 3 days 7 days Eucalyptus 100.08 4.46 8.35 8.15 1.1 Hinoki 100.07 4.45 5.9 47.84 4.2 Tea Tree 100.07 5.05 6.45 2.85 0.1

[0102] For the purpose of carrying out the invention thereafter, eucalyptus was used as an example.

[0103]

[0104] Example 1. Preparation of a polymer support layer loaded with an antibacterial component

[0105] As shown in Fig. 1, PDMS monomer, crosslinking agent, eucalyptus essential oil as an antibacterial oil, and petrolatum as a release rate regulator were added to a container and mixed. The mixture was then placed in a mold and cured to produce a polymer support layer loaded with an antibacterial component. As in Experimental Examples 1 and 2, polymer support layers loaded with an antibacterial component were produced by varying the content ratio of each component and used in the experiments.

[0106]

[0107] Experimental Example 1. Release delay effect according to vaseline content

[0108] As in Example 1, the ratio of the content of eucalyptus essential oil and vaseline was adjusted, and a polymer support layer containing PDMS in an amount 2.5 times the weight of the sum of the two components was manufactured. Then, the weight of eucalyptus essential oil remaining in the polymer support layer over time was measured to evaluate the release delay effect according to the content of vaseline, and the results are presented in Table 3 below.

[0109] Time (h) Sample Weight (E: Eucalyptus, V: Vaseline, wt%)E:V = 7:3E:V = 5:5E:V = 3:70100.0100.0100.0100.0199.199.599.5298.999.199.22094.095.196.82493.094.796.39683.384.191.012082.782.689.914479.981.489.016876.780.388.3

[0110] According to Table 3 above, the higher the oil (eucalyptus) content, the higher the release rate, and it can be seen that Vaseline has the effect of delaying the release of oil.

[0111]

[0112] Experimental Example 2. Release control effect according to PDMS content

[0113] The release characteristics of oil (antibacterial component) were evaluated by varying the content ratio of antibacterial component in the polymer of the polymer support layer. Vaseline, as a release rate control agent, was used at a weight ratio of 1:1 to the oil.

[0114] The results are presented in Table 4 below.

[0115] Oil Residual (wt%) PDMS: Oil: Vaseline 0 hr 2 hr 1 day 7 days 14 days Surface Oil Exposure 10:2:2100.099.396.092.992.6X5:2:2100.099.094.489.388.9X2:2:2100.098.692.684.583.8O

[0116] According to Table 4 above, as the PDMS content increases, the oil release rate slows down. In the case of a 2:2:2 ratio, although oil and Vaseline are contained in large quantities and the oil is retained within the structure, it can be confirmed that the oil is exposed on the surface and continuously leaks out.

[0117]

[0118] Example 2. Spray coating of polymer composition

[0119] The following experiment was conducted to confirm the spray coating performance of the antimicrobial polymer composition according to Manufacturing Example 1. Figure 2 shows the spray coating performed in this example.

[0120] Specifically, 10 wt% of the antibacterial polymer manufactured in Manufacturing Example 1 was dissolved in methyl ethyl ketone (MEK) and acetone, respectively, to make the coating solution below, and spray coating was performed on the polymer support layer, as shown in Fig. 3. At this time, the solvent used for coating is not particularly limited as long as it is a low-boiling-point solvent capable of dissolving the polymer, but since the polymer of Manufacturing Example 1 shows particularly good solubility in ketone-based solvents, methyl ethyl ketone was used as the solvent.

[0121] For spray coating, the nozzle used was an AM6 nozzle from ATOMAX. The nozzle spray spacing was fixed at 5 mm, and the nozzle-to-sample distance was 15 cm. Coating was performed twice on a 2 cm × 2 cm PDMS polymer, with an interval of 1 minute between coats. The nozzle moving speed was 15 mm / min, and the air pressure of the nozzle was 2 kPa. The solution concentration was 10%, the solution discharge rate was 1 ml / min, and the sample temperature was 27°C.

[0122] As described above, a structure having antifungal, antibacterial, and antifungal properties was manufactured by spray coating an antibacterial polymer on a polymer support layer, and its schematic diagram is shown in Fig. 4.

[0123]

[0124] Experimental Example 3. Evaluation of Surface Properties of Antibacterial Structures

[0125] (1) Surface structure analysis

[0126] In order to evaluate the coating surface properties of the polymer compound according to Manufacturing Example 1, the surface microstructure was observed using a scanning electron microscope after spray coating, and the results are shown in FIGS. 5 to 8.

[0127] Figures 5 and 6 are microstructure photographs taken at 200x and 500x magnification using an optical microscope after spray coating, and Figures 7 and 8 show microstructure photographs taken at 1,000x and 10,000x magnification using a scanning electron microscope.

[0128] As a result of the analysis, it was confirmed that fibers of several hundred nanometers in thickness were well formed, as shown in FIGS. 7 and 8, and similar results were observed when acetone was used as a solvent. This confirms that the polymer compound according to Manufacturing Example 1 can well form a coating layer through spray coating.

[0129]

[0130] (2) Contact angle analysis

[0131] In order to evaluate the coating surface properties of the polymer compound according to Manufacturing Example 1, 10 wt% of the polymer compound of Manufacturing Example 1 was dissolved in methyl ethyl ketone (MEK) to make a coating solution. The coating solution was coated under the following three conditions, and the contact angle for water of each coated surface was measured, which is shown in Fig. 9. Fig. 9a shows spray coating on a polymer support layer that does not contain oil (antibacterial component), Fig. 9b shows spray coating after plasma treatment to improve polymer adhesion to the polymer support layer, and Fig. 9c shows spray coating on a polymer support layer containing oil (antibacterial component). As a result, the contact angles were all measured to be 150° or higher.

[0132]

[0133] (3) Confirmation of release of oil (antibacterial component) through contact

[0134] In order to confirm that oil inside the polymer support layer can be released to the outside even after surface coating with a polymer compound, the structure was touched with a finger and oil was confirmed to be released through the structure, as shown in Fig. 10.

[0135] Figure 10 (a) is a photograph of a sticker spray-coated with the polymer compound of Example 1 on a polymer support layer, and when it was touched as in (b), it was seen that it came off on the hand as in (c) and that antibacterial oil was released to the outside as in (d).

[0136]

[0137] Experimental Example 4. Evaluation of the mechanical durability of antibacterial structures (85℃, 85RH%)

[0138] Samples under various conditions according to Table 5 were subjected to an 85 / 85 durability test to determine whether the polymer was decomposed or deformed through weight changes and changes in contact angle with water for 72 hours at 85°C and 85% humidity. Two samples of the oil-supported structural film claimed in the present invention were produced and designated as structural films #1 and #2.

[0139] Sample Test Before Test After Comparison Weight (g) Weight (g) Difference (g) Change (%) PDMS Film 0.4102 0.4211 0.0109 2.7 Oil-loaded PDMS Film 0.4126 0.3745-0.038-9.2 Oil-free Structure Film 0.4644 0.4739 0.0095 2.0 Structure Film #10.4635 0.4251-0.038-8.3 Structure Film #20.4695 0.4269-0.043-9.1

[0140] (1) Weight change before and after 85 / 85 durability evaluation

[0141] In the case of the control PDMS film, when there is no oil, it tends to slightly increase in weight due to absorption of some moisture, but when oil is impregnated, it is understood that the oil is released due to high temperature and the weight decreases. This can be confirmed that in the structure with the same spray-coated coating layer added, when there is no oil impregnated, the weight increases somewhat due to moisture absorption, and in the case of structure films #1 and #2, the weight decreases. Through this, it was confirmed that there was no effect on the collapse or decomposition of the structure in terms of weight, and in comparison with the control group, the overall increase or decrease in weight before and after the evaluation is not much different from that evaluated in the general PDMS film, so it can be seen that there is no big change in the durability or in terms of oil evaporation and water absorption in forming the structure.

[0142] (2) Contact angle for water before and after 85 / 85 durability evaluation

[0143] The contact angle for water before and after the 85 / 85 test of the structure film #1 is shown in Fig. 11.

[0144] Figure 11a shows a photograph before the 85 / 85 test, and Figures 11b and 11c show the results after two 85 / 85 tests. The increase in each contact angle was at most 0.3 degrees, showing little change, indicating that the durability of the structural film was maintained even after the test.

[0145]

[0146] Experimental Example 5. Antibacterial Evaluation of Antibacterial Polymer Layers

[0147] (1) Preparation of bacterial solution of test strain

[0148] The test strains used were Staphylococcus aureus ATCC 6538 (S. aureus), a Gram-positive bacterium, Escherichia coli ATCC 8739 (E. coli), a Gram-negative bacterium, and Candida albicans ATCC 10231 (C. albicans). Bacteria were inoculated onto Nutrient Agar (NA) and pre-cultured at 35°C for 1 day, and yeasts were inoculated onto Potato Dextrose Agar (PDA) and pre-cultured at 25°C for 2 days. The pre-cultured strains were diluted in sterile saline solution to prepare a bacterial solution with a concentration of 106 CFU / mL.

[0149] (2) Antibacterial test

[0150] A film was manufactured using the polymer according to Manufacturing Example 1, and its antibacterial activity was evaluated. A film cut to 5 cm × 5 cm (width × height) was placed on the bottom of a petri dish, and 0.4 mL of bacterial solution was inoculated into each dish. A sterilized film cut to 4 cm × 4 cm (width × height) was covered on top, and cultured at 35°C for 24 hours. A sterilized film was used as the film placed on the bottom for the control group. After culture, the film inoculated with bacteria was homogenized into 10 mL of SCDLP medium, and the film inoculated with yeast was homogenized into 10 mL of SDLPA medium, and the bacterial count was measured.

[0151] The results of measuring antibacterial activity are shown in Table 6 below.

[0152] S. aureusE. coliC. albicans0 h24 h0 h24 h0 h24 hControl2.4×10 5 3.5×10 5 1.9×10 5 2.3×10 7 2.4×10 5 1.1×10 5 PFPMA-QDMA< 10 1 < 10 1 < 10 1

[0153] The test results for all three test strains showed an inhibition rate of over 99.9%, indicating that the antibacterial polymer has high antibacterial power.

Claims

1. A polymer support layer containing an antibacterial ingredient; and An antimicrobial structure comprising an antimicrobial polymer layer coated on the support layer.

2. In paragraph 1, The above antibacterial component is a volatile, antibacterial structure.

3. In paragraph 1, The above antibacterial component is an antibacterial structure containing an antibacterial plant essential oil.

4. In paragraph 1, An antimicrobial structure wherein the polymer support layer further comprises a release rate controlling agent.

5. In paragraph 1, The above antibacterial polymer layer is an antibacterial structure comprising an antibacterial fluorine-based polymer.

6. In paragraph 5, The above antibacterial fluorine-based polymer is an antibacterial structure in which a vinyl monomer containing a quaternary ammonium ion is grafted onto the fluorine-based polymer main chain.

7. In paragraph 5, The above antibacterial fluorine-based polymer is an antibacterial structure in which a fluorine-based (meth)acrylic monomer is further grafted onto the fluorine-based polymer main chain.

8. In paragraph 5, An antimicrobial structure wherein the above antimicrobial fluorinated polymer is a copolymer further comprising a repeating unit derived from one of VDF, TFE, trifluoroethylene, and HFP.

9. In paragraph 7, The vinyl monomer containing the above quaternary ammonium ion is an antibacterial structure having the following chemical formula 1: [Chemical Formula 1] In the above chemical formula 1, R1 to R3 are each independently C- 1-4 It is alkyl; Xmore Cl - , Br - I - AlCl4 - Al2Cl7 - , BF4 - PF6 - ClO4 - NO3 - CH3COO - CF3COO - CH3SO3 - CF3SO3 - (CF3SO2)2N - (CF3SO2)3C - AsF6 - SbF6 - , NbF6 - TaF6 - (CN)2N - C4F9SO3 - (C2F5SO2)2N - C3F7COO - More (CF3SO2)(CF3CO)N - 이고; X is H or CH3, Y- is any anion, The above n is 1 to 6.

10. In paragraph 1, The above antibacterial polymer layer is an antibacterial structure in which the antibacterial polymer forms a three-dimensional network in the form of fibers.

11. Step of forming a polymer support layer containing an antibacterial component; Comprising a step of coating an antibacterial polymer layer on a support layer, A method for manufacturing an antibacterial structure of the first paragraph.

12. In paragraph 11, The polymer support layer comprises a step of polymerizing a monomer for polymer formation so as to include a crosslinking agent and an antibacterial component; and further comprising a step of crosslinking the polymerized polymer, A method for manufacturing an antibacterial structure of the first paragraph.

13. Heterogeneous film layer; an adhesive layer formed on a release film; and An antibacterial substrate comprising the antibacterial structure of claim 1, wherein a polymer support layer is laminated on an adhesive layer.

14. Step of removing the release film layer of the antibacterial substrate; and A method of using an antibacterial substrate, comprising a step of adhering an antibacterial substrate from which a heteromorphic film layer has been removed to a surface of a target object.

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