Pseudomonas arvensis sp. nov.

Abstract

The invention relates to an agronomic composition comprising a Pseudomonas sivasensis or a Pseudomonas arvensis sp. nov., uses of this composition and use of a Pseudomonas sivasensis or a Pseudomonas arvensis sp. nov. as a protective endophyte.

Description

[0001] PSEUDOMONAS ARVENSIS SP. NOV.

[0002] technical field

[0003] The present invention relates to varieties of Pseudomonas sp. having biocontrol or biostimulation properties and their use in agronomy.

[0004] Previous art

[0005] Many microorganisms are known to have potentially interesting properties in biocontrol or biostimulation.

[0006] Among these, different strains of Pseudomonas sp. have been identified in the rhizosphere of rapeseed plants, or associated with other ecosystems.

[0007] However, the genus Pseudomonas includes a great many species, expressing, depending on their species, or even depending on the strain, an immense diversity of combinations of factors associated with properties that are sometimes useful, sometimes deleterious, sometimes useful under certain conditions and deleterious under others.

[0008] Patent application CN1 13817653 describes the Pseudomonas fluorescens strain BsEBl, which is useful for improving the survival rate of tissue-cultured Bletilla striata plants after transplantation and for promoting Bletilla striata growth. However, there are no observations regarding a potential protective effect against pathogens.

[0009] Thus, generalization or extrapolation to the level of the Pseudomonas species is meaningless; conversely, the identification of markers associated with beneficial species, or even strains, represents a more readily applicable technical contribution. Brief summary of the invention

[0010] A first aspect of the present invention relates to an agronomic composition comprising a strain of Pseudomonas sp. having more than 99.6% identity with SEQ.ID.NO:1 or SEQ.ID.NO:2 on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2, preferably this Pseudomonas sp. expresses koreencein and / or fragine and / or contains the functional genes kecA, kecB, kecC, kecD, kecE, kecF, kecG, kecH, kecl, kecJ, kecK, and / or the functional genes hamA, hamB, hamC, hamD and hamE.

[0011] Preferably, Pseudomonas comprises operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 (SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40).

[0012] Alternatively, or furthermore, preferably, Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20 and SEQ ID NO:21 and, preferably, SEQ ID NO:22 (SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52 and SEQ ID NO:53 and, preferably, SEQ ID NO:54).

[0013] Alternatively, or furthermore, preferably, Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28 and SEQ ID NO:29 (SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45 and SEQ ID NO:46 and, preferably, SEQ ID NO:47).

[0014] This composition is advantageous for the biocontrol of pathogens, preferably insects or fungi. An associated aspect of the present invention relates to an agronomic composition comprising an extract of this Pseudomonas as described above.

[0015] Another associated aspect of the present invention relates to a seed of a plant of interest coated with an artificial composition, comprising the agronomic composition or the agronomic composition comprising the extract described above.

[0016] Preferably, it is a liquid composition, preferably aqueous.

[0017] Advantageously, this composition is for spraying on a plant of interest.

[0018] Thus, a related aspect of the present invention relates to a method for preventing or treating disease affecting a plant, comprising the step of applying one of the compositions described above to a plant of interest.

[0019] Preferably, the plant is a monocotyledon, preferably a cereal, preferably chosen from wheat, barley, spelt, rye, rice and maize.

[0020] Preferably, the disease is a fungal disease, preferably caused by Fusarium (preferably Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici).

[0021] This method is also advantageous when the disease is caused by an insect.

[0022] Another aspect of the present invention relates to a granulated composition comprising a carrier and one of the agronomic compositions described above. Preferably, the carrier is selected from a silicate and / or an aluminosilicate, for example kaolin or vermiculite, a calcium salt, for example gypsum, dolomite, talc, a polysaccharide, for example maltodextrin, a lignin derivative, or a mixture thereof.

[0023] Advantageously, in these compositions, Pseudomonas is encapsulated in a water-sensitive matrix, preferably a polysaccharide matrix, such as alginate or xanthan gum.

[0024] Another related aspect of the present invention relates to the use of a strain of Pseudomonas or a composition comprising a strain of Pseudomonas or an extract of a strain of Pseudomonas, said strain of Pseudomonas having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 for the biocontrol of pathogens, preferably fungal pathogens and / or pathogens affecting a monocotyledon, preferably the pathogen is chosen from Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici.

[0025] Another associated aspect of the present invention relates to the use of a strain of Pseudomonas having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 as an endophyte.

[0026] Preferably, for these uses, Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:5, SEO ID NO:6, SEO ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 (SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40).

[0027] Preferably, for these uses, Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20 and SEQ ID NO:21 and, preferably, SEQ ID NO:22 (SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52 and SEQ ID NO:53 and, preferably, SEQ ID NO:54).

[0028] Preferably, for these uses, Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28 and SEQ ID NO:29 (SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45 and SEQ ID NO:46 and, preferably, SEQ ID NO:47).

[0029] Another related aspect of the present invention relates to the use of a Pseudomonas strain or a composition comprising a Pseudomonas strain or an extract of a Pseudomonas strain, said Pseudomonas strain having more than 90% identity with SEQ ID NO:1 or 2, over the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity over at least 10,000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4, for the biocontrol of insect pests, wherein the Pseudomonas comprises the operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:1, SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 ... :10, SEQ ID NO:1 1, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 (SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO :38, SEQ ID NO:39, SEQ ID NO:40).Brief description of the drawings.

[0030] Figure 1 shows the colonization of wheat by a Pseudomonas according to the invention.

[0031] Detailed description of an embodiment of the invention

[0032] The inventors identified a group within the genus Pseudomonas for the biocontrol of pathogens and for increased growth.

[0033] These Pseudomonas share a very strong sequence identity and have the common characteristic of producing koreencein (B) and / or fragine (in the context of the present invention, it is preferably (-)-Fragine).

[0034] Formula I (non-limiting example) - Koreencein B

[0035] Other Koreenceins are known, such as Koreencein A (molecular mass, 208 Da), C (similar to B, but hydroxylated on the carbon chain; molecular mass 226 Da) and D (molecular mass 278 Da).

[0036] Formula 2 - (-)-Fragine

[0037] Furthermore, the Pseudomonas species of the present invention synthesize at least one siderophore, cellulase activity, and protease activity. Pseudomonas sivasensis and Pseudomonas arvensis are among the identified Pseudomonas species. Based on genetic analyses, the inventors have grouped some of the strains possessing the properties described above into a subspecies of P. arvensis.

[0038] The inventors have further demonstrated the colonization of plants at the tissue level, and even of the vascular system, by these Pseudomonas, which provides a more lasting systemic effect; this also allows for use directly at the seed level and / or as an endophyte.

[0039] Thus, a first aspect of the present invention relates to an agronomic composition comprising a strain of Pseudomonas sp. having more than 99.6% identity with SEQ.ID.NO:1 or SEQ.ID.NO:2 on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2.

[0040] These are the sequences between (joining) 16S and 23S, which are particularly conserved.

[0041] Advantageously, these strains of Pseudomonas express koreencein (at least one of the forms, although form B is shown above, this is not limiting, although preferred) and / or fragin, or at least have the operons to do so.

[0042] Thus, preferably, the Pseudomonas strain includes the functional operons kecA, kecB, kecC, kecD, kecE, kecF, kecG, kecH, kecl, kecl, kecJ and kecK for the production of koreencein (B).

[0043] In the context of the present invention, the KecA-K series is preferably understood to mean:

[0044] - KecA - Acyl group-bearing protein

[0045] - KecB & kecD - B-ketoacyl synthases

[0046] - KecC & kecE - “thiolase-like” domains

[0047] - kecF -pyridoxalphosphate- / acyl / ACP-dependent aminotransferase

[0048] - kecG - ketoreductase kegH - NAD(P) H oxidoreductase

[0049] Kecl-kecJ encode hypothetical proteins

[0050] KecK encodes a carrier

[0051] In the context of the present invention, kecK is potentially less important.

[0052] Thus, preferably, the Pseudomonas strain includes the functional operons hamA, hamB, hamC, hamD, hamE, hamF, and hamG, (all functional sequences except possibly hamF and hamG) for the production of fragin.

[0053] In the context of the present invention, the hamA-F series preferably means:

[0054] - HamA - An oxygenase-like with a hemic group

[0055] - HamB - a protein similar to RmlC with a cupin domain

[0056] - HamC - p-aminobenzoate N-oxygensa AurF

[0057] - HamE - Polyketide cyclase-dehydrase

[0058] - HamG - Class III Aminotransferase

[0059] - HamD includes an adenylation domain, a thiolation domain and a reductase domain.

[0060] The inventors have, moreover, sequenced the genes of the Koreencein operon in the strains of Pseudomonas according to the invention; these are sequences 5 to 15.

[0061] Similarly, for two different species, belonging to two different genera, the Fragine operons in the strains of Pseudomonas according to the invention were sequenced: SEQ ID NOs 16 to 22 (P. s / vasens / s) and SEQ ID Nos: 23 to 29 (P. arvensis, strain DS3PS4).

[0062] Thus, preferably, a Pseudomonas according to the present invention encodes peptides having at least 95% identity with each of the SEQ ID NOs: 5 to 15 (translated into peptides; cf. SEQ ID Nos: 30 to 40), and / or (i) peptides having at least 95% identity with each of the SEQ ID NOs: 16 to 22 (translated into peptides; SEQ ID Nos: 48 to 54) or (ii) peptides having at least 95% identity with each of the SEQ ID NOs: 23 to 29 (translated into peptides; SEQ ID NOs: 41 to 47).

[0063] In the context of the present invention, the percentage of identity is preferably calculated on the basis of the sequence in question over its entire length (possibly translated) and calculated according to the BLAST algorithm with standard parameters (BLOSUM matrix 62; size of "word"=3); it is the sum of the retained residues and those marked by a "+" sign.

[0064] Furthermore, and advantageously, the identified protein is functional.

[0065] In the context of the present invention, "functional protein" is preferably understood to mean a protein having the percentage of identity described above and an absence of an internal "STOP" codon, which would result in a protein truncated by more than 5% of its length.

[0066] The presence of these operons advantageously implies the ability of Pseudomonas to synthesize fragine and / or Koreencein (B).

[0067] An associated aspect of the present invention relates to an agronomic composition comprising an extract of Pseudomonas as described above, for example comprising fragine and / or Koreencein (B).

[0068] In addition to these two biomolecules, the extract according to the invention advantageously comprises other effective biomolecules, even those synergistic with fragine and / or koreencein. Furthermore, the presence of the functional operons fragine and / or koreencein in a Pseudomonas (having an identity with SEQ ID Nos. 1-4 as described above) implies a close phylogenetic relationship, even if the Pseudomonas had been classified in another species.

[0069] An associated aspect of the present invention relates to a seed of a plant of interest coated with an artificial composition, comprising the agronomic composition described above. Preferably, the incorporation of the agronomic composition into the coating and subsequent treatments are compatible with the viability of Pseudomonas.

[0070] Given the ability of Pseudomonas to colonize the tissues of the plant of interest, its incorporation into the seed will reinforce this, to the benefit of the plant.

[0071] Preferably, alternatively, the composition(s) comprising the Pseudomonas described above is a liquid composition.

[0072] This allows, for example, spraying on a plant of interest.

[0073] An associated aspect of the present invention relates to a method for preventing or treating disease affecting a plant, comprising the step of applying the composition described above to a plant of interest, preferably a monocotyledon, preferably a cereal, preferably selected from wheat, barley, spelt, rye, rice and maize.

[0074] This composition is particularly effective when the disease is a fungal disease, or even when the pathogen is an insect (in the presence of the functional koreencein operon), and is preferably caused by Fusarium sp., preferably Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici.

[0075] An alternative to the present invention relates to a granulated composition comprising a support and the agronomic composition(s) described above.

[0076] Preferably, the support is chosen from a silicate and / or an aluminosilicate, for example kaolin or vermiculite, a calcium salt, for example gypsum, dolomite or talc, - a polysaccharide, for example maltodextrin or even an alginate or xanthan gum, which even allows the encapsulation of Pseudomonas for delayed release, a lignin derivative,

[0077] - or a mixture of these.

[0078] Another related aspect of the present invention relates to the use of a strain of Pseudomonas having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 for the biocontrol of insect pests or fungal pathogens and / or monocotyledon pathogens, preferably the pathogen is chosen from Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici.

[0079] Besides the strain, a composition including this Pseudomonas or, better still, an extract of this Pseudomonas is advantageous for this use.

[0080] Another related aspect of the present invention relates to the use of a strain of Pseudomonas having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 as an endophyte (Pseudomonas having the ability to colonize plant tissues).

[0081] In these applications, Pseudomonas preferably expresses fragin and / or koreencein (B) and / or possesses the (functional) operons of sequences 5-15 and / or those of sequences 16-22 or 23-29, i.e., they encode functional peptides having at least 95% identity with these sequences, identity being calculated as described above. Other features and advantages of the present invention will be derived from the following non-limiting description, and with reference to the drawings and examples.

[0082] Examples.-

[0083] It is understood that the present invention is in no way limited to the embodiments described above and that many modifications can be made to it without departing from the scope of the annexed claims.

[0084] Example 1: Collection of stumps:

[0085] The inventors analyzed the microbiome of four cereal fields located in Belgium: 3 fields of winter wheat and one of spelt.

[0086] An initial pre-screening test for microorganisms was performed by testing for antagonism with Fusarium graminearum. This was followed by an in vitro antagonism test against four pathogens: Fusarium graminearum, Gaeumannomyces graminis var. tritici, Oculimacula yallundae, and Zymoseptoria tritici.

[0087] In addition, the genomes were sequenced and subjected to MLSA "Multi Locus Sequence analysis", with sequencing of 16S rRNA and other genes (gapA, glnS, gltA, gyrB, ileS, nuoD, recA, rpoB and rpoD).

[0088] Furthermore, the presence of cellulolytic or proteolytic activities was tested. Example 2 - identification of promising strains.

[0089] 444 isolates were obtained. Among these, 114 showed antagonism against Fusarium graminearum, and 33 of these showed antagonism against at least 3 of the 4 pathogens described in Example 1.

[0090] All isolates showed cellulase activity, protease activity and the ability to produce a siderophore.

[0091] Among these isolates, the inventors noted, to their great surprise, a significant proportion of species of the Pseudomonas sivasensis type. And Pseudomonas arvensis.

[0092] These were therefore sequenced (see SEQ ID NO: 3 and SEO ID NO: 4). There was no plasmid carried by these strains.

[0093] Based on the sequencing, the inventors were able to propose a new subspecies, closely related to P. arvensis.

[0094] These two groups share the expression of genes involved in biofilm formation such as algA, algG, algl, algDEFJKLPQRUWX, mucABDP, Ukwn (antigen-O) and wcaG (but not algCZ, algB, mucCE, the rhamnose synthesis gene rhIA (but not rhIBC), several genes involved in type IV pili, Pyochelin and Pyoverdine receptors, as well as pyoverdine, and secondary metabolism molecules such as viscosine, APE-Vf, pseudopyronin, fragine (especially sp. Nov) and koreencein (especially P. sivasensis species).

[0095] Example 3 - Colonization of plants of interest

[0096] The inventors soaked wheat seeds in solutions containing Pseudomonas sivasensis or Pseudomonas arvensis sp. Nov. according to the invention and labeled with green fluorescent protein (GFP). The inventors observed significant systemic colonization by confocal microscopy (Figure 1a). The experiment was repeated, this time by spraying onto the leaves, and tissue colonization was observed, particularly in the stomatal niches (Figure 1b).

[0097] Example 4 - in planta protection The Pseudomonas from Example 3 (non-GFP) were sprayed on wheat leaves just before Z. tritici infection or 2 and 3 days before infection. The number of lesions decreased sharply (negative control, 0; positive control, 50; Pseudomonas before infection: 20; Pseudomonas days 2 and 3: 5), as did the average number of pycnidia (negative control, 0; positive control, 97; Pseudomonas before infection: 30; Pseudomonas 2 and 3 days before infection: not detected).

Claims

Demands 1. An agronomic composition comprising a strain of Pseudomonas sp. having more than 99.6% identity with SEQ.ID.NO :1 or SEQ.ID.NO :2 on the 4639 nucleotides of said SEQ ID NO :1 or SEQ ID NO :

2.

2. The composition according to claim 1 being for the biocontrol of pathogens, preferably an insect or fungus.

3. The agronomic composition according to claim 1 or 2, wherein the Pseudomonas strain expresses koreencein and / or includes the functional genes kecA, kecB, kecC, kecD, kecE, kecF, kecG, kecH, kecl, kecJ, kecK.

4. The composition according to any of the preceding claims wherein Pseudomonas expresses fragin and / or comprises the functional genes hamA, hamB, hamC, hamD and hamE.

5. The composition according to any of the preceding claims wherein Pseudomonas comprises operons encoding peptides having at least 95% identity with each of the sequences SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, and SEQ ID NO:

40.

6. The composition according to any one of the preceding claims wherein Pseudomonas comprises operons encoding peptides having at least 95% identity with each of the sequences SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52 and SEQ ID NO:53 and, preferably, SEQ ID NO:

54.

7. The composition according to any one of the preceding claims 1 to 5 wherein Pseudomonas comprises the operons encoding peptides having at least 95% identity with each of the sequences SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45 and SEQ ID NO:46 and, preferably, SEQ ID NO:

47.

8. An agronomic composition comprising an extract of Pseudomonas as defined in claims 1 to 7.

9. A seed of a plant of interest coated with an artificial composition, said artificial composition comprising the agronomic composition according to any one of claims 1 to 8.

10. The composition according to any one of claims 1 to 8 being a liquid composition. 1 1. The liquid composition of claim 10 being for spraying on a plant of interest.

12. A method for preventing or treating a disease affecting a plant comprising the step of applying the composition according to claim 10 or 11 to a plant of interest.

13. The method according to claim 12 wherein the plant is a monocotyledon, preferably a cereal, preferably selected from wheat, barley, spelt, rye, rice and maize.

14. The method according to claim 12 or 13 wherein the disease is a fungal disease, preferably caused by Fusarium, preferably Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici.

15. The method according to claim 12 or 13 wherein the disease is caused by an insect.

16. A granulated composition comprising a carrier and the agronomic composition according to any one of claims 1 to 8.

17. The composition according to claim 16, wherein the support is selected from - a silicate and / or an aluminosilicate, for example kaolin or vermiculite, - a calcium salt, for example gypsum, dolomite, talc, - a polysaccharide, for example maltodextrin, - a derivative of lignin, or - a mixture of these.

18. The composition according to any one of claims 16 to 17, wherein Pseudomonas is encapsulated in a matrix sensitive to water, preferably, a polysaccharide matrix, such as alginate or xanthan gum.

19. Use of a Pseudomonas strain or a composition comprising a Pseudomonas strain or an extract of a Pseudomonas strain, said Pseudomonas strain having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 for the biocontrol of pathogens, preferably fungal pathogens and / or pathogens affecting a monocotyledon, preferably the pathogen is selected from Fusarium graminearum, Gaeumannomyces graminis, Oculimacula yallundae and Zymoseptoria tritici.

20. Use of a Pseudomonas strain having more than 90% identity with SEQ ID NO:1 or 2, on the 4639 nucleotides of said SEQ ID NO:1 or SEQ ID NO:2 and / or more than 95% identity on at least 10000 consecutive nucleotides of SEQ ID NO:3 or SEQ ID NO:4 as an endophyte.

21. Use according to claims 19 or 20, wherein Pseudomonas comprises operons encoding peptides having at least 95% identity with SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39 and SEQ ID NO:

40.

22. Use according to any one of claims 19 to 21, wherein Pseudomonas comprises operons encoding peptides having at least 95% identity with each of the sequences SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52 and SEQ ID NO:53 and, preferably, SEQ ID NO:

54.

23. Use according to any one of claims 19 to 21, wherein Pseudomonas comprises operons encoding peptides having at least 95% identity with the translation product of each of the sequences SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28 and SEQ ID NO:

29.

24. Use of a Pseudomonas strain or a composition comprising a Pseudomonas strain or an extract of a Pseudomonas strain, said Pseudomonas strain having more than 90% identity with SEQ ID NO: 1 or 2, over the 4639 nucleotides of said SEQ ID NO: 1 or SEQ ID NO: 2 and / or more than 95% identity over at least 10000 consecutive nucleotides of SEQ ID NO: 3 or SEQ ID NO: 4, for the biocontrol of insect pests, wherein the Pseudomonas comprises the operons encoding peptides having at least 95% identity with SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39 and SEQ ID NO:40.

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