Nutritional composition and pet food for improving pet health

Abstract

A nutritional composition for improving pet health and a pet food, the nutritional composition being composed of dietary fibers, probiotics and oil. The dietary fibers comprise blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder; the probiotics are at least one of Bacillus coagulans MTCC5856, Enterococcus faecalis GDMCC No: 62869, Bifidobacterium longum GDMCC No: 62866, and Enterococcus faecium GDMCC No: 62870; the oil is at least one of fish oil, animal oil and vegetable oil, and the oil contains ω-3 and ω-6 polyunsaturated fatty acids. The provided nutritional composition for improving pet health can improve the intestinal health of pets, reduce the rate of diarrhea, enhance the immune and antioxidant capacity of pets, and improve the hair health of pets.

Description

Nutritional composition for improving pet health and pet food TECHNICAL FIELD

[0001] The present application relates to the technical field of pet feed, in particular to a nutritional composition for improving pet health and pet food. BACKGROUND

[0002] In daily life, pets are becoming more and more important in people's hearts. How to make their pets grow healthily, meet the daily needs of pets through daily diet, increase the body's immunity and disease resistance, and reduce the occurrence of diseases are the topics that pet owners are concerned about at present. However, for pets with various problems in gastrointestinal digestion and absorption function, the food fed usually has a great influence on the state of the pets. Cat and dog food, as a kind of nutritional food specially provided for cats and dogs, belongs to a kind of high-end pet food between human food and traditional livestock and poultry feed, and the main purpose is to provide pets with basic life guarantee and growth and development nutrients. Therefore, pet nutritional composition and pet food are required to have the characteristics of being able to supplement the nutrients required for daily life activities, high digestion and absorption rate, scientific formula, and being able to prevent cat and dog diseases.

[0003] At present, there are few compositions on the market that can improve pet health in many aspects, and the composition is relatively simple and the function is not comprehensive enough.

[0004] SUMMARY

[0005] The technical problem to be solved by the present application is to provide a nutritional composition for improving pet health, which can improve the intestinal health of pets, reduce the diarrhea rate, enhance the antioxidant capacity and immunity of pets, and improve the hair health of pets.

[0006] In order to solve the above technical problems, the present application provides the following technical scheme:

[0007] The first aspect of the present application provides a nutritional composition for improving pet health, which is composed of dietary fiber, probiotics and oil, wherein:

[0008] The dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder;

[0009] The probiotics are at least one of Bacillus coagulans MTCC5856, Enterococcus faecalis GDMCC No:62869, Bifidobacterium longum GDMCC No:62866 and Enterococcus faecium GDMCC No:62870;

[0010] The oil is at least one of fish oil, animal oil and vegetable oil, and the oil contains polyunsaturated fatty acid omega-3 and polyunsaturated fatty acid omega-6.

[0011] Further, the nutritional composition comprises the following components in parts by weight: dietary fiber 6-15 parts, polyunsaturated fatty acid omega-3 8-12 parts, polyunsaturated fatty acid omega-6 21-39 parts.

[0012] Further, in the nutritional composition, the dietary fiber comprises the following components in the following amounts: blueberry powder 0.2-3 parts, cranberry powder 0.2-3 parts, pumpkin powder 0.5-4 parts, carrot powder 4-9 parts, chicory powder 0.1-2 parts.

[0013] Further, in the nutritional composition, the dietary fiber comprises the following components in the following amounts: blueberry powder 0.2-3 parts, cranberry powder 0.2-3 parts, pumpkin powder 0.5-4 parts, carrot powder 4-9 parts, chicory powder 0.1-2 parts.

[0014] Further, in the nutritional composition, the probiotic bacteria are selected from any one of the following groups:

[0015] a. Bacillus coagulans MTCC5856 in an amount of (1-5)*10 6 CFU / g;

[0016] b. Enterococcus faecium GDMCC No:62869 in an amount of (0.5-6.5)*10 9 CFU / g;

[0017] c. Enterococcus faecium GDMCC No:62869, Bifidobacterium longum GDMCC No:62866 and Enterococcus faecalis GDMCC No:62870 in a total amount of (0.4-2.5)*10 10 CFU / g.

[0018] Further, in the nutritional composition, the probiotic bacteria are selected from any one of the following groups:

[0019] a. Bacillus coagulans MTCC5856 in an amount of 3.88*10 6 CFU / g;

[0020] b. Enterococcus faecium GDMCC No:62869 in an amount of (1.94-4.85)*10 9 CFU / g;

[0021] c. Enterococcus faecium GDMCC No:62869, Bifidobacterium longum GDMCC No:62866 and Enterococcus faecalis GDMCC No:62870 in a total amount of (0.58-1.46)*10 10 CFU / g.

[0022] Further, the ratio of polyunsaturated fatty acid omega-3 to omega-6 in the nutritional composition is 1:3.

[0023] Further, the content of polyunsaturated fatty acid omega-3 in the nutritional composition is 10 parts, and the content of polyunsaturated fatty acid omega-6 is 30 parts.

[0024] The second aspect of the present application provides the use of the above-mentioned nutritional composition in the preparation of pet food.

[0025] The third aspect of the present application provides a pet food, which comprises the above-mentioned nutritional composition.

[0026] Compared with the prior art, the present application has the following beneficial effects:

[0027] 1. The nutritional composition for improving the health of pets provided by the present application comprises dietary fiber, probiotics and oil, wherein the dietary fiber is composed of blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder, can regulate the growth and distribution of intestinal flora, improve antioxidant capacity and improve intestinal health; the probiotics are at least one of Bacillus coagulans MTCC5856, Enterococcus faecalis GDMCC No:62869, Bifidobacterium longum GDMCC No:62866 and Enterococcus faecalis GDMCC No:62870, which can enhance the digestion and absorption of nutrients and immune function in the intestinal tract; and the ratio of polyunsaturated fatty acid omega-3 to omega-6 is controlled by the oil, which can improve the dandruff and lackluster condition of pet hair and improve the quality of hair.

[0028] 2. In the nutritional composition for improving the health of pets provided by the present application, the dietary fiber, probiotics and oil produce a synergistic effect, and when they are used together, they can better improve the intestinal health of pets, improve the digestive function and reduce the incidence of diarrhea; and improve the antioxidant capacity and immune capacity of pets and improve the health of pet hair. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0030] As described in the background, for pet food, it is required to have the characteristics of comprehensive nutrition, high digestion and absorption rate, scientific formula, and the ability to treat or prevent cat and dog diseases. However, at present, there are few compositions on the market that can improve pets in many ways, and the combination is relatively simple and the function is not comprehensive enough.

[0031] To solve the technical problem, the present application provides a nutritional composition for improving pet health, which has rich nutritional ingredients, can improve intestinal health of pets, improve hair health of pets, and improve immunity of pets.

[0032] Specifically, the nutritional composition provided by the present application is composed of three types of ingredients, namely dietary fiber, probiotics and oil.

[0033] Dietary fiber is a polysaccharide that cannot be digested and absorbed by the gastrointestinal tract, nor can it produce energy. Dietary fiber can be specifically divided into two categories, namely soluble dietary fiber and insoluble dietary fiber. Soluble dietary fiber is generally derived from pectin, alginate, konjac, etc., which is intertwined with starch and other carbohydrates in the gastrointestinal tract and delays the absorption of the latter, so it can reduce postprandial blood glucose. Insoluble dietary fiber is generally derived from whole grain food, including wheat bran, oatmeal, brown rice, oat whole grain food, beans, vegetables and fruits, etc. Insoluble dietary fiber can promote gastrointestinal peristalsis, speed up the passage of food through the gastrointestinal tract, and reduce absorption; in addition, insoluble fiber absorbs water in the large intestine, softens the stool, and can prevent and treat constipation.

[0034] In the present application, the dietary fiber in the nutritional composition can be provided by fruits, vegetables and other raw materials. Preferably, these fruits and vegetables can be dried to make powder, and the dietary fiber can be provided by the fruit and vegetable powder, so that the amount of dietary fiber added can be accurately controlled.

[0035] In the present application, the source of dietary fiber includes two categories. The first category is pumpkin powder, carrot powder and chrysanthemum powder. This type of fruit and vegetable powder can provide rich dietary fiber, which can not only strengthen intestinal peristalsis and improve the digestibility of nutrients, but also regulate the abundance of intestinal flora, promote the production of intestinal mucus, improve the integrity of intestinal mucosa, and improve intestinal health. The second category is blueberry powder and cranberry powder. Blueberry and cranberry are berries originally from North America. Blueberry contains rich anthocyanins, and cranberry contains rich proanthocyanidins. These ingredients have strong antioxidant activity, can scavenge free radicals, improve the activity of antioxidant enzymes, and inhibit lipid peroxidation, which can reduce intestinal damage caused by oxidative stress, regulate intestinal barrier function, and improve intestinal flora structure, thereby further improving intestinal health. In addition, blueberry powder and cranberry powder can also promote the growth of intestinal beneficial bacteria, maintain the balance of intestinal microecology of pets, further improve the intestinal health level of pets, and enhance the ability of pets to resist pathogenic bacteria and harmful microorganisms.

[0036] In some embodiments of the present application, the amount of dietary fiber added in the nutritional composition can be 6-15 parts, for example, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.

[0037] In some embodiments of the present application, the amount of blueberry powder added to the nutritional composition is 0.2-3 parts, for example, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.8 parts, 3.0 parts, etc., preferably 0.8-1.2 parts, and further preferably 1 part.

[0038] In some embodiments of the present application, the amount of cranberry powder added to the nutritional composition is 0.2-3 parts, for example, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.8 parts, 3.0 parts, etc., preferably 0.8-1.2 parts, and further preferably 1 part.

[0039] In some embodiments of the present application, the amount of pumpkin powder added to the nutritional composition is 0.5-4 parts, for example, 0.5 parts, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.4 parts, 3.6 parts, 3.8 parts, 4 parts, etc., preferably 1.8-2.5 parts, and further preferably 2 parts.

[0040] In some embodiments of the present application, the amount of carrot powder added to the nutritional composition is 4-9 parts, for example, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, etc., preferably 6-8 parts, and further preferably 7 parts.

[0041] In some embodiments of the present application, the amount of chicory powder added to the nutritional composition is 0.1-2 parts, for example, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, 1.2 parts, 1.5 parts, 1.6 parts, 1.8 parts, 2 parts, etc., preferably 0.4-0.8 parts, and further preferably 0.5 parts.

[0042] In some preferred embodiments of the present application, the amounts of the components of dietary fiber added to the nutritional composition are as follows: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, and 0.5 parts of chicory powder. The use of this ratio of dietary fiber can better affect the growth and distribution of intestinal flora and improve intestinal health.

[0043] Probiotics are active microorganisms that colonize in the body of human or animal, and can change the composition of the flora in a certain part of the host. Adding a certain amount of probiotics in pet food can enhance the immune regulation ability of pets, inhibit the adhesion of pathogenic bacteria to intestinal villi, improve the barrier function of the digestive system epithelium, and improve the digestive problems of pets.

[0044] There are many types of probiotics applied in pet food, such as yeast, Bacillus subtilis, Bifidobacterium, Lactobacillus, cheese probiotics, etc. Unlike the probiotics commonly used in the prior art, the inventors have selected several specific bacteria as probiotics after long-term experiments, and the probiotics are at least one of Bacillus coagulans MTCC5856, Enterococcus faecalis GDMCC No:62869, Bifidobacterium longum GDMCC No:62866, and Enterococcus faecium GDMCC No:62870.

[0045] Bacillus coagulans MTCC5856 is a gram-positive spore-forming bacteria, which has the ability to improve digestive function, improve nutritional status, and enhance immunity.

[0046] Enterococcus faecalis can form a biofilm on intestinal epithelial cells, protecting the intestinal epithelial cells from harmful substances; it also has nutritional effects, can promote the absorption of amino acids and residues, and improve the synthesis of tissue proteins.

[0047] Bifidobacterium longum can effectively inhibit intestinal pathogenic bacteria, thereby effectively relieving gastrointestinal diseases such as irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD), regulating intestinal flora imbalance, and improving symptoms such as abdominal discomfort, abdominal distension, and difficulty defecating; in addition, it plays an important role in maintaining the intestinal microecological balance of the body and regulating the immune capacity of the body.

[0048] Enterococcus faecium is a gram-positive coccus, which can inhibit the adhesion of pathogenic bacteria to the intestine through the size exclusion effect, form an intestinal barrier, protect the intestinal health, and maintain the microecological balance; can inhibit the reproduction of animal pathogenic bacteria, maintain and adjust the intestinal microecological balance; produce various nutrients (vitamins, amino acids, growth-promoting factors, etc.), which can participate in metabolism and promote animal growth; can induce the body to produce cytokines, interferons, interleukins, etc., enhance the non-specific immunity of the body, improve the disease resistance, reduce the inflammatory response, and promote the intestinal health.

[0049] The inventors have found that, compared with other types of probiotics, the several probiotics selected by the present application, when used alone or in combination, can better promote the intestinal health of pets and improve the digestive problems of pets when compounded with dietary fiber and oil as a nutritional composition.

[0050] In the present nutritional composition, the probiotic bacteria can be added in the form of bacterial powder and the live bacterial addition can be in the range of 1*10 5 CFU / g, for example, it can be 1.0*10 10 CFU / g, 5*10 5 CFU / g, 1*10 5 CFU / g, 5*10 6 CFU / g, 1*10 6 CFU / g, 5*10 7 CFU / g, 1*10 7 CFU / g, 5*10 8 CFU / g, 1*10 8 CFU / g, 5*10 9 CFU / g, 1*10 9 CFU / g, 5*10 10 CFU / g, 1*10 10 CFU / g, 2*10 10 CFU / g, 2.5*10 6 CFU / g, etc.

[0051] In some preferred embodiments of the present application, the probiotic bacteria and their addition can be any of the following groups:

[0052] a. Bacillus coagulans MTCC 5856 in the range of (1-5)*10 6 CFU / g, for example, it can be 1*10 6 CFU / g, 2*10 6 CFU / g, 3*10 6 CFU / g, 4*10 6 CFU / g, 5*10 6 CFU / g, etc., preferably 3.88*10 9 CFU / g;

[0053] b. Enterococcus faecalis GDMCC No: 62869 in the range of (0.5-6.5)*10 9 CFU / g, for example, it can be 0.5*10 9 CFU / g, 1*10 9 CFU / g, 1.5*10 9 CFU / g, 2*10 9 CFU / g, 2.5*10 9 CFU / g, 3*10 9 CFU / g, 3.5*10 9 CFU / g, 4*10 9CFU / g, 5*10 9 CFU / g, 5.5*10 9 CFU / g, 6*10 9 CFU / g, 6.5*10 9 The preferred concentration of CFU / g is (1.94~4.85)*10. 9 CFU / g;

[0054] c. Enterococcus faecalis GDMCC No:62869, Bifidobacterium longum GDMCC No:62866, and Enterococcus faecalis GDMCC No:62870, with a total addition amount of (0.4~2.5)*10. 10 CFU / g, for example, can be 0.4*10 10 CFU / g, 0.5*10 10 CFU / g, 0.6*10 10 CFU / g, 0.8*10 10 CFU / g, 1.0*10 10 CFU / g, 1.2*10 10 CFU / g, 1.4*10 10 CFU / g, 1.6*10 10 CFU / g, 1.8*10 10 CFU / g, 2.0*10 10 CFU / g, 2.2*10 10 CFU / g, 2.4*10 10 CFU / g, 2.5*10 10 The preferred concentration of CFU / g is (0.58~1.46)*10. 10 CFU / g; preferably, the addition ratio of the above three bacteria is 1:1:1.

[0055] Oils are also a major nutrient in pet food. They provide pets with essential fats, improve palatability, and enhance coat health. Oils can be divided into saturated and unsaturated fatty acids, with unsaturated fatty acids being an essential type of fatty acid for humans. Based on the number of double bonds, unsaturated fatty acids are further classified into monounsaturated and polyunsaturated fatty acids. Among dietary fats, monounsaturated fatty acids include oleic acid, while polyunsaturated fatty acids include linoleic acid, linolenic acid, and arachidonic acid. Humans and animals cannot synthesize linoleic acid and linolenic acid and must obtain them from their diet. Based on the position and function of the double bonds, polyunsaturated fatty acids are further divided into ω-3 and ω-6 series. Linolenic acid, DHA, and EPA belong to the ω-3 series, while linoleic acid and arachidonic acid belong to the ω-6 series.

[0056] Adding ω-3 and ω-6 series polyunsaturated fatty acids in pet food is beneficial to improve the condition of pet hair dandruff and lack of luster, and improve the quality of hair. Meanwhile, the proportion of ω-3 and ω-6 should be controlled in a certain range. According to reports, when the proportion of ω-3 and ω-6 is between 1:2 and 1:10, it has a positive effect on inflammation, skin allergy, arthritis, certain tumors and other diseases, and can improve skin health. At present, it is generally believed that the optimal proportion of ω-3 and ω-6 series polyunsaturated fatty acids is 1:4.

[0057] In the present application, the inventors have found through a large number of experiments that when the proportion of polyunsaturated fatty acids ω-3 and ω-6 in the nutritional composition is controlled to be 1:(2.1-3.9), it is more beneficial to the health of pets. Pets fed with pet food with this proportion of polyunsaturated fatty acids show obvious advantages in hair grade, immune indicators and blood lipid indicators, etc. In the preferred embodiments, the proportion of polyunsaturated fatty acids ω-3 and ω-6 is 1:3, which is most beneficial to the health of pets.

[0058] In some embodiments of the present application, the content of polyunsaturated fatty acid ω-3 in the nutritional composition is 8-12 parts, for example, it can be 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, etc.; the content of polyunsaturated fatty acid ω-6 is 21-39 parts, for example, it can be 21 parts, 23 parts, 1:25 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 35 parts, 36 parts, 38 parts, 39 parts, etc.

[0059] In the present application, the types of oil and fat in the nutritional composition include but are not limited to at least one of fish oil, animal oil and vegetable oil. Among them, fish oil can be selected from salmon oil, cod oil, tuna oil and other oil extracted from deep-sea animals, which is rich in ω-3 series polyunsaturated fatty acids DHA, EPA, etc. Animal oil can be selected from chicken oil, beef tallow and other animal fat, which is mainly composed of saturated fatty acids. Vegetable oil can be selected from flaxseed oil, sunflower seed oil and other vegetable fat, which is rich in ω-6 series polyunsaturated fatty acid linoleic acid, and flaxseed oil is also rich in ω-3 series polyunsaturated fatty acid α-linolenic acid.

[0060] According to the types of polyunsaturated fatty acids rich in the above-mentioned oil and fat, by selecting appropriate oil and fat, the proportion of ω-3 and ω-6 series polyunsaturated fatty acids in the nutritional composition can be controlled.

[0061] The nutritional composition provided by the present application can be obtained by mixing dietary fiber, oil and bacteria powder. The nutritional composition is preferably in the form of a powder bag, which can be added to various pet food raw materials in a certain proportion to obtain pet food. In some embodiments, the amount of the nutritional composition added to the pet food can be 4% to 6%. Compared with the pet food raw materials, the nutritional composition can improve the overall nutritional composition of the pet food, improve the intestinal health of pets, and improve the digestion, immunity and antioxidant capacity of pets.

[0062] It should be noted that the pet food raw material described above can be a pet basic food (also known as a base food), which contains some of the most basic nutrients required by pets, such as protein, vitamins, minerals, and part of the oil, etc. For example, in some embodiments, the base food can include the following components by mass percentage: crude protein 30.0% to 50.0%, crude fat 9.0% to 25.0%, crude fiber ≤ 9%, moisture ≤ 14%, calcium 0.6% to 1.3%, total phosphorus 0.5% to 1.2%, taurine 0.1% to 0.2%. In addition, such base food can also be obtained by commercial purchase, such as Good Taste Complete Cat Food.

[0063] The present application will be further described below in conjunction with specific examples and comparative examples.

[0064] In each of the following examples and comparative examples, the base food is Good Taste Complete Cat Food, and the raw material composition thereof is: meat and its products (chicken powder 15%), wheat, corn, corn protein powder, beer yeast powder, fish oil (1.0%), taste enhancer (1.0%). The composition of the additive is: vitamin A, vitamin D3, dl-α-tocopherol acetate, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 12, D-biotin, folic acid, nicotinamide, D-calcium pantothenate, choline chloride, zinc sulfate, copper sulfate, ferrous sulfate, manganese sulfate, sodium selenite, calcium iodate, potassium chloride, taurine, tert-butyl hydroquinone, citric acid, light calcium carbonate, calcium hydrogen phosphate. The ingredient test results are shown in Table 1, and each component is calculated on a dry basis, except for moisture and oil.

[0065] Table 1

[0066] In each of the following examples and comparative examples, Bacillus coagulans MTCC5856 is obtained by commercial purchase, Bacillus subtilis is purchased from Jinan Xingyihe Chemical Co., Ltd., Lactobacillus (Lactobacillus plantarum) is purchased from Jiangsu Caolei Biological Technology Co., Ltd., and Bifidobacterium is purchased from Shandong Jiahongyike Biological Engineering Co., Ltd.

[0067] Enterococcus faecalis HHP003 (Enterococcus faecalis) is Enterococcus faecalis A-A-2 with the preservation number GDMCC No:62869, which was preserved in Guangdong Microbial Culture Collection Center on October 13, 2022;

[0068] Bifidobacterium longum HHP004 (Bifidobacterium longum) is Bifidobacterium longum A-1-12 with the preservation number GDMCC No:62866, which was preserved in Guangdong Microbial Culture Collection Center on May 9, 2023;

[0069] Enterococcus faecium HHP005 (Enterococcus faecium) is Enterococcus faecium M-3 with the preservation number GDMCC No:62870, which was preserved in Guangdong Microbial Culture Collection Center on October 13, 2022.

[0070] Example 1

[0071] The nutritional composition for improving the health of pets provided in this embodiment is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder, the probiotics are Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil.

[0072] The content of each component in the nutritional composition is: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable count of Bacillus coagulans MTCC5856 is 3.88*10 6 CFU / g.

[0073] 5.15g of the nutritional composition of this embodiment is added to the base food, mixed, to obtain 100g of finished pet food. The content of the nutritional composition in the finished pet food is: 0.1wt% of blueberry powder, 0.1wt% of cranberry powder, 0.2wt% of pumpkin powder, 0.7wt% of carrot powder, and 0.05wt% of chicory powder; the viable count of Bacillus coagulans MTCC5856 is 2*10 5 CFU / g.

[0074] Example 2

[0075] The nutritional composition for improving the health of pets provided in this embodiment is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder, the probiotics are Enterococcus faecalis HHP003, and the oil is chicken oil and fish oil.

[0076] The content of each component in the nutritional composition is: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable bacterial amount of Enterococcus faecalis HHP003 is 3.88*10 9 CFU / g.

[0077] 5.15 g of the nutritional composition of the present example is added to the base food, mixed, and 100 g of finished pet food is obtained. The content of the nutritional composition in the finished pet food is: 0.1 wt% of blueberry powder, 0.1 wt% of cranberry powder, 0.2 wt% of pumpkin powder, 0.7 wt% of carrot powder, and 0.05 wt% of chicory powder; the total viable bacterial amount of Enterococcus faecalis HHP003, Bifidobacterium longum HHP004, and Enterococcus faecium HHP005 is 5*10 8 CFU / g.

[0078] Example 3

[0079] The nutritional composition for improving the health of pets provided in the present example is composed of dietary fiber, probiotics, and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, and chicory powder, the probiotics are Enterococcus faecalis HHP003 + Bifidobacterium longum HHP004 + Enterococcus faecium HHP005, and the oil is chicken oil and fish oil.

[0080] The content of each component in the nutritional composition is: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable bacterial amount of Enterococcus faecalis HHP003, Bifidobacterium longum HHP004, and Enterococcus faecium HHP005 is 3.23*10 9 CFU / g.

[0081] 5.15 g of the nutritional composition of the present example is added to the base food, mixed, and 100 g of finished pet food is obtained. The content of the nutritional composition in the finished pet food is: 0.1 wt% of blueberry powder, 0.1 wt% of cranberry powder, 0.2 wt% of pumpkin powder, 0.7 wt% of carrot powder, and 0.05 wt% of chicory powder; the total viable bacterial amount of Enterococcus faecalis HHP003, Bifidobacterium longum HHP004, and Enterococcus faecium HHP005 is 5*10 8 CFU / g.

[0082] Comparative Example 1

[0083] The nutritional composition for improving the health of pets provided in the present example is composed of probiotics, wherein the probiotics are Bacillus coagulans MTCC5856.

[0084] The probiotic of the present comparative example was added to the base diet and mixed to obtain 100 g of the finished pet food. The viable count of Bacillus coagulans MTCC 5856 in the finished pet food was 2*10 5 CFU / g.

[0085] Comparative Example 2

[0086] The nutritional composition for improving the health of pets provided in the present comparative example consists of dietary fibers, wherein the dietary fibers include blueberry powder, cranberry powder, pumpkin powder, carrot powder, and chicory powder.

[0087] The content of each component in the nutritional composition was as follows: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, and 0.5 parts of chicory powder.

[0088] 1.15 g of the nutritional composition of the present comparative example was added to the base diet and mixed to obtain 100 g of the finished pet food. The content of the nutritional composition in the finished pet food was as follows: 0.1 wt% of blueberry powder, 0.1 wt% of cranberry powder, 0.2 wt% of pumpkin powder, 0.7 wt% of carrot powder, and 0.05 wt% of chicory powder.

[0089] Comparative Example 3

[0090] The nutritional composition for improving the health of pets provided in the present comparative example consists of oils, wherein the oils are chicken oil and fish oil.

[0091] The content of each component in the nutritional composition was as follows: 10 parts of polyunsaturated fatty acid omega-3 and 30 parts of polyunsaturated fatty acid omega-6.

[0092] 4 g of the nutritional composition of the present comparative example was added to the base diet and mixed to obtain 100 g of the finished pet food.

[0093] Comparative Example 4

[0094] The nutritional composition for improving the health of pets provided in the present comparative example consists of dietary fibers, probiotics, and oils, wherein the dietary fibers include blueberry powder, pumpkin powder, and chicory powder, the probiotics are Bacillus coagulans MTCC 5856, and the oils are chicken oil and fish oil.

[0095] The content of each component in the nutritional composition was as follows: 1 part of blueberry powder, 2 parts of pumpkin powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable count of Bacillus coagulans MTCC 5856 was 3.88*10 6 CFU / g.

[0096] 5.15 g of the nutritional composition of the present comparative example (including 0.8 g of corn starch) was added to the base food and mixed to obtain 100 g of the finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1 wt%, pumpkin powder 0.2 wt%, chicory powder 0.05 wt%, corn starch 0.8 wt%, and the viable cell count of Bacillus coagulans MTCC5856 was 2*10 5 CFU / g.

[0097] Comparative Example 5

[0098] The nutritional composition provided by the present comparative example for improving the health of pets is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes cranberry powder, carrot powder, chicory powder, the probiotics are Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil.

[0099] The content of each component in the nutritional composition was: 1 part of cranberry powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable cell count of Bacillus coagulans MTCC5856 was 3.88*10 6 CFU / g.

[0100] 5.15 g of the nutritional composition of the present comparative example (including 0.3 g of corn starch) was added to the base food and mixed to obtain 100 g of the finished pet food. The content of the nutritional composition in the finished pet food was: cranberry powder 0.1 wt%, carrot powder 0.7 wt%, chicory powder 0.05 wt%, corn starch 0.3 wt%, and the viable cell count of Bacillus coagulans MTCC5856 was 2*10 5 CFU / g.

[0101] Comparative Example 6

[0102] The nutritional composition provided by the present comparative example for improving the health of pets is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, and chicory powder, the probiotics are Bacillus subtilis, and the oil is chicken oil and fish oil.

[0103] The content of each component in the nutritional composition was: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable cell count of Bacillus subtilis was 3.88*10 6 CFU / g.

[0104] 5.15g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100g of finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1wt%, cranberry powder 0.1wt%, pumpkin powder 0.2wt%, carrot powder 0.7wt%, chicory powder 0.05wt%, and the viable bacterial count of Lactobacillus plantarum was 2*10 5 CFU / g.

[0105] Comparative Example 7

[0106] The nutritional composition for improving pet health provided by the present comparative example is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Lactobacillus plantarum, and the oil is chicken oil and fish oil.

[0107] The content of each component in the nutritional composition was: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; and the viable bacterial count of Lactobacillus plantarum was 3.88*10 6 CFU / g.

[0108] 5.15g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100g of finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1wt%, cranberry powder 0.1wt%, pumpkin powder 0.2wt%, carrot powder 0.7wt%, chicory powder 0.05wt%, and the viable bacterial count of Lactobacillus plantarum was 2*10 5 CFU / g.

[0109] Comparative Example 8

[0110] The nutritional composition for improving pet health provided by the present comparative example is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Bifidobacterium, and the oil is chicken oil and fish oil.

[0111] The content of each component in the nutritional composition was: 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, 0.5 parts of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; and the viable bacterial count of Bifidobacterium was 3.88*10 6 CFU / g.

[0112] 5.15 g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100 g of the finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1 wt%, cranberry powder 0.1 wt%, pumpkin powder 0.2 wt%, carrot powder 0.7 wt%, chicory powder 0.05 wt%, and the viable count of Bacillus coagulans MTCC5856 was 2*10 5 CFU / g.

[0113] Comparative Example 9

[0114] The nutritional composition for improving the health of pets provided by the present comparative example is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil.

[0115] The content of each component in the nutritional composition was: blueberry powder 1 part, cranberry powder 1 part, pumpkin powder 2 parts, carrot powder 7 parts, chicory powder 0.5 parts, polyunsaturated fatty acid omega-3 20 parts, polyunsaturated fatty acid omega-6 20 parts; and the viable count of Bacillus coagulans MTCC5856 was 3.88*10 6 CFU / g.

[0116] 5.15 g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100 g of the finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1 wt%, cranberry powder 0.1 wt%, pumpkin powder 0.2 wt%, carrot powder 0.7 wt%, chicory powder 0.05 wt%, and the viable count of Bacillus coagulans MTCC5856 was 2*10 5 CFU / g.

[0117] Comparative Example 10

[0118] The nutritional composition for improving the health of pets provided by the present comparative example is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil.

[0119] The content of each component in the nutritional composition was: blueberry powder 1 part, cranberry powder 1 part, pumpkin powder 2 parts, carrot powder 7 parts, chicory powder 0.5 parts, polyunsaturated fatty acid omega-3 16 parts, polyunsaturated fatty acid omega-6 24 parts; and the viable count of Bacillus coagulans MTCC5856 was 3.88*10 6 CFU / g.

[0120] 5.15 g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1 wt%, cranberry powder 0.1 wt%, pumpkin powder 0.2 wt%, carrot powder 0.7 wt%, chicory powder 0.05 wt%, and the viable count of Bacillus coagulans MTCC 5856 was 2*10 5 CFU / g.

[0121] Comparative Example 11

[0122] The present comparative example provides a nutritional composition for improving the health of pets, which is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Bacillus coagulans MTCC 5856, and the oil is chicken oil and fish oil.

[0123] The content of each component in the nutritional composition was: blueberry powder 1 part, cranberry powder 1 part, pumpkin powder 2 parts, carrot powder 7 parts, chicory powder 0.5 parts, polyunsaturated fatty acid ω-3 13.3 parts, polyunsaturated fatty acid ω-6 26.7 parts; and the viable count of Bacillus coagulans MTCC 5856 was 3.88*10 6 CFU / g.

[0124] 5.15 g of the nutritional composition of the present comparative example was added to the base food and mixed to obtain 100 g of finished pet food. The content of the nutritional composition in the finished pet food was: blueberry powder 0.1 wt%, cranberry powder 0.1 wt%, pumpkin powder 0.2 wt%, carrot powder 0.7 wt%, chicory powder 0.05 wt%, and the viable count of Bacillus coagulans MTCC 5856 was 2*10 5 CFU / g.

[0125] Comparative Example 12

[0126] The present comparative example provides a nutritional composition for improving the health of pets, which is composed of dietary fiber, probiotics and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotics are Bacillus coagulans MTCC 5856, and the oil is chicken oil and fish oil.

[0127] The content of each component in the nutritional composition was: blueberry powder 0.5 parts, cranberry powder 0.5 parts, pumpkin powder 1 part, carrot powder 3.5 parts, chicory powder 0.25 parts, polyunsaturated fatty acid ω-3 5 parts, polyunsaturated fatty acid ω-6 15 parts; and the viable count of Bacillus coagulans MTCC 5856 was 3.88*10 6 CFU / g.

[0128] Example 1 provides a nutritional composition for improving pet health, which is composed of dietary fiber, probiotic bacteria and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotic bacteria is Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil. 5 CFU / g.

[0129] Comparative Example 13

[0130] The nutritional composition provided by the comparative example for improving pet health is composed of dietary fiber, probiotic bacteria and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotic bacteria is Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil.

[0131] The content of each component in the nutritional composition is: 2 parts of blueberry powder, 2 parts of cranberry powder, 4 parts of pumpkin powder, 14 parts of carrot powder, 1 part of chicory powder, 10 parts of polyunsaturated fatty acid omega-3, and 30 parts of polyunsaturated fatty acid omega-6; the viable bacterial amount of Bacillus coagulans MTCC5856 is 3.88*10 6 CFU / g.

[0132] Example 1 provides a nutritional composition for improving pet health, which is composed of dietary fiber, probiotic bacteria and oil, wherein the dietary fiber includes blueberry powder, cranberry powder, pumpkin powder, carrot powder, chicory powder, the probiotic bacteria is Bacillus coagulans MTCC5856, and the oil is chicken oil and fish oil. 5 CFU / g.

[0133] Test and Test

[0134] Experimental method: healthy adult cats (male to female ratio 1:1) with similar age and weight were selected, randomly divided into groups, 6 in each group, and fed in single cages. The pet foods prepared by the examples and comparative examples were fed respectively. The feed was given according to the recommendation of NRC (2006). The animals were allowed to eat and drink freely, and the adaptation period was 1 week, and the test period was 8 weeks. At specific time points, feces, blood or hair were collected for the following scoring or testing.

[0135] 1. Fecal quality

[0136] The feces were scored according to Table 2, and the average value was taken (the closer to 3, the better).

[0137] Table 2

[0138] The diarrhea rate was calculated by the number of diarrhea during the test period: Diarrhea rate (%) = 100 x the number of diarrhea of the cat during the test period / the total number of feces score statistics of the cat during the test period.

[0139] 2. Nutrient apparent digestibility

[0140] All feces were collected continuously for 5 days at the 6th week, weighed and stored in a -20℃ freezer for analysis. The contents of crude protein (GB / T 6432-2018), dry matter (GB / T 6435-2014), crude fat (GB / T 6433-2006), crude fiber (GB / T 6434-2006), crude ash (GB / T 6438-2007), calcium (GB / T 6436-2018) and phosphorus (GB / T 6437-2018) in the feed and feces samples were determined. The total energy of the feed and feces samples was determined using an oxygen bomb calorimeter (ISO 9831:1998). The formula for calculating the apparent digestibility of a nutrient is as follows:

[0141] Apparent digestibility of a nutrient (%) = (nutrient content in feed - nutrient content in feces) / (nutrient content in feed) x 100.

[0142] 3. Preparation of blood samples and determination of biochemical indicators

[0143] At the end of the test, the cats in the test and control groups were subjected to venous blood sampling from the forelimbs. Part of the blood was subjected to routine blood analysis, and the rest was separated into serum for blood biochemical detection and immunoglobulin detection. The blood biochemical detection indicators included blood glucose Glu, blood lipid TG, high-density lipoprotein HDLC, low-density lipoprotein LDLC, total protein TP, albumin ALB, globulin GLOB, glutamic-pyruvic transaminase ALT, glutamic-oxaloacetic transaminase AST; the immunoglobulin detection indicators included IgA, IgG and IgM. The immunoglobulin IgA, IgG and IgM were determined using an Elisa kit.

[0144] Among the above blood biochemical indicators, the blood glucose was better between 4.5 and 14.0, the blood lipid was better between 17.0 and 104.0; the high-density lipoprotein HDLC was better with higher value, and the low-density lipoprotein LDLC was better with lower value; the total protein was better between 54.00 and 89.00, the albumin was better between 22.00 and 45.00, and the globulin was better between 15.00 and 57.00; the glutamic-pyruvic transaminase was better between 8.2 and 100, and a higher value would cause liver damage; the glutamic-oxaloacetic transaminase was better between 9.2 and 39.5, and a higher value would cause liver damage.

[0145] 4. Blood antioxidant indicators

[0146] The determination of serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) activity all used the kit of Nanjing Jiancheng Biological Engineering Institute, and the determination method was operated according to the instruction.

[0147] Among the above blood antioxidant indexes, the higher the superoxide dismutase SOD is within a certain range, the better; the lower the malondialdehyde MDA is within a certain range, the better, and the higher indicates that the degree of cell membrane lipid peroxidation is high; the higher the total antioxidant capacity T-AOC is within a certain range, the better, and the higher the antioxidant capacity is.

[0148] 5. Hair health

[0149] On the 42nd day of the test, the back hair of each cat was collected, and the fur gloss and softness indicators of the cats in each group were evaluated by 5 professionals according to the fur condition evaluation standard (the closer to 3, the better) in a double-blind manner. All samples were scored using the following 5 grades: 1 = dull, rough, dry; 2 = moderate reflection, moderate softness; 3 = high reflection, very soft; 4 = moderate softness, moderate oiliness; 5 = very oily, where 1≤HS≤2 is dry, 2<HS<4 is ideal, and 4≤HS≤5 is oily.

[0150] 6. Data processing and analysis

[0151] Independent sample t test was used in SPSS 26.0 software, and the results were expressed as "mean ± standard error". Letters represent significant differences, and different letters indicate significant differences between groups. Single letter shows strong significant difference, i.e. p<0.01; double letter shows weak significant difference, i.e. p<0.05.

[0152] Test Example 1: Effect of probiotic species on pet health

[0153] Thirty-six healthy adult cats (male:female ratio 1:1) with similar age and weight were selected and randomly divided into 6 groups, 6 cats in each group, and fed in single cages. The feed was formulated according to the recommendation of NRC (2006) to meet the daily nutritional level of the test cats. Free access to food and water, adaptation period for 1 week, test period for 8 weeks. Feces were collected daily, and feces scoring was performed by 5 observers, and the average value was calculated, and the diarrhea rate was calculated. The apparent digestibility of nutrients was detected after continuous collection of feces for 5 days in the 8th week, and the average value was obtained. The results are shown in Table 3.

[0154] Table 3

[0155] As can be seen from Table 3, the cats fed with the pet food of Examples 1-3 have better fecal scores than the cats of Comparative Examples 6-8, and the diarrhea rates are significantly lower than those of Comparative Examples 6-8. In addition, the dry matter digestibility, crude protein digestibility, crude fat digestibility, phosphorus digestibility, crude ash digestibility, crude fiber digestibility, calcium digestibility and digestible energy are all better than those of Comparative Examples 6-8. In addition, in the immune health index, Examples 1-3 are also generally better than Comparative Examples 6-8. Therefore, compared with the commonly used probiotics such as Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium, the selection of Bacillus coagulans MTCC5856, Enterococcus faecalis HHP003 and Enterococcus faecalis HHP003 + Bifidobacterium longum HHP004 + Enterococcus faecium HHP005 as probiotics in the nutritional composition of the present application can better improve the intestinal health of pets, improve the digestive problems of pets and improve the immune health of pets.

[0156] Test Example 2: Influence of the type of dietary fiber on pet health

[0157] Eighteen healthy adult cats with similar age and weight (male to female ratio 1:1) were selected and randomly divided into 3 groups, 6 cats in each group, and fed in single cages. The feed was prepared according to the NRC (2006) recommendation to meet the daily nutritional level of the test cats. The cats were allowed to freely eat and drink water, and the adaptation period was 1 week, and the test period was 8 weeks. Feces were collected daily, and fecal scores were evaluated by 5 observers, and the average value was calculated, and the diarrhea rate was calculated. The apparent digestibility of nutrients was obtained by averaging the values after detection of the feces collected for 5 consecutive days at the 8th week. The forelimb venous blood of the cats was collected at the end of the experiment for antioxidant index analysis. The results are shown in Table 4.

[0158] Table 4

[0159] As can be seen from the results of Table 4, the fecal score of the dietary fiber of Example 1 using blueberry powder + cranberry powder + pumpkin powder + carrot powder + chicory powder is better than that of Comparative Examples 4-5, and the diarrhea rate is significantly lower than that of Comparative Examples 4-5. The same results are also shown in the digestibility indexes, and the dry matter digestibility, crude protein digestibility, crude fat digestibility, phosphorus digestibility, crude ash digestibility, crude fiber digestibility, calcium digestibility and digestible energy of Example 1 are all higher than those of Comparative Examples 4-5. In addition, the antioxidant index of Example 1 is also significantly better than that of Comparative Examples 4-5. This indicates that the selection of blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder as dietary fiber in the nutritional composition of the present application can better improve the intestinal health, improve the digestive capacity and the antioxidant capacity.

[0160] Test Example 3: Influence of the ratio of polyunsaturated fatty acids ω-3:ω-6 on pet health

[0161] Select 24 healthy adult cats (male:female ratio 1 :1) with similar age and weight, and randomly divide them into 4 groups, 6 cats in each group, for single cage feeding. The feed is prepared according to the recommendation of NRC (2006) to meet the daily nutritional level of the test cats. The cats are allowed to freely eat and drink, and the adaptation period is 1 week, and the test period is 8 weeks. At the end of the experiment, 5 observers score the hair grade of the test cats and take the average value; at the same time, the pre-experimental forelimb venous blood is collected for analysis of immune protein and antioxidant biochemical indicators. The results are shown in Table 5.

[0162] Table 5

[0163] As can be seen from the results in Table 5, compared with Comparative Examples 9-11, the hair score of the pets in Example 1 is closest to 3, the hair is highly reflective, and very soft. In addition, Example 1 is lower than Comparative Examples 9-11 in blood lipid and low-density lipoprotein indicators, and higher than Comparative Examples 9-11 in high-density lipoprotein indicators, indicating that the blood health of the pets in Example 1 is better than that of Comparative Examples 9-11. This shows that when the polyunsaturated fatty acid ω-3:ω-6 in the pet food is controlled to be 1:3, it is most beneficial to the health of the pets.

[0164] Test Example 4: Effect of Nutritional Composition Ingredients on Pet Health

[0165] Select 24 healthy adult cats (male:female ratio 1 :1) with similar age and weight, and randomly divide them into 4 groups, 6 cats in each group, for single cage feeding, and feed the pet food of Comparative Examples 1-3 and Example 1 respectively. The feed is prepared according to the recommendation of NRC (2006) to meet the daily nutritional level of the test cats. The cats are allowed to freely eat and drink, and the adaptation period is 1 week, and the test period is 8 weeks. The feces are collected daily, scored by 5 observers, and the diarrhea rate is calculated. The apparent digestibility of nutrients is detected by continuously collecting feces for 5 days in the 8th week. At the end of the experiment, 5 observers score the hair grade of the test cats and take the average value; at the same time, the pre-experimental forelimb venous blood is collected for analysis of immune protein and antioxidant biochemical indicators. The results are shown in Table 6.

[0166] Table 6

[0167] As can be seen from the results in Table 6, in Comparative Examples 1-3, the use of probiotics, dietary fiber or oil alone in the nutritional composition cannot comprehensively improve the health of the pets. For example, in Comparative Example 1, the use of probiotics alone in the nutritional composition can effectively improve the digestion of the pets and reduce the diarrhea rate, but it has no obvious effect on the improvement of the immune capacity, antioxidant capacity and hair health of the pets. In Comparative Example 2, the use of dietary fiber alone in the nutritional composition can significantly improve the antioxidant capacity of the pets, but it has no obvious effect on the improvement of the digestion of the pets, the reduction of the diarrhea rate, and the improvement of the hair health of the pets.

[0168] As a comparison, the nutritional composition in Example 1 simultaneously uses probiotics, dietary fiber and oil, and the results show that the intestinal health, antioxidant capacity, immune capacity and hair health of the pets are significantly improved. In particular, from the results in the table, it can also be seen that the pets in Example 1 have certain improvement in the partial digestibility index, immune index and hair health index relative to Comparative Examples 1-3, which indicates that when the nutritional composition simultaneously contains probiotics, dietary fiber and oil, the three components can play a synergistic effect, which can better improve the intestinal health of the pets, improve the immune capacity, and improve the hair health of the pets.

[0169] Test Example 5: Effect of the amount of the nutritional composition on the health of pets

[0170] Eighteen healthy adult cats with similar age and weight (male:female = 1:1) were selected and randomly divided into 3 groups, 6 cats in each group, and single-cage feeding was performed. The feed was prepared according to the NRC (2006) recommendation to meet the daily nutritional level of the test cats. Free access to food and water, the adaptation period was 1 week, and the test period was 8 weeks. The feces were collected daily, and the feces were scored by 5 observers, and the average value was calculated, and the diarrhea rate was calculated. The apparent digestibility of nutrients was obtained by taking the average value of the values obtained by detecting the feces collected for 5 consecutive days at the 8th week. The front limb venous blood of the cats was collected at the end of the experiment for immune protein and antioxidant index analysis, and the hair index was scored by 5 observers on the hair grade of the experimental cats, and the average value was taken. The results are shown in Table 7.

[0171] Table 7

[0172] As shown in Table 7, in Comparative Example 12, the amount of the nutritional composition added to the pet food is less, and it is not as good as the example in improving intestinal health, improving digestive capacity, improving immune capacity and antioxidant capacity, and improving hair health. In Comparative Example 13, the amount of the nutritional composition added to the pet food is more. From the results, the excessive addition of the nutritional composition has a negative effect on many indicators relative to Example 1, such as a significant increase in the diarrhea rate relative to Example 1, and a significant decrease in the digestive capacity; in addition, there is a certain decrease in the immune capacity and antioxidant capacity. Therefore, the amount of the nutritional composition in the pet food needs to be controlled within a certain range, and the amount that is too low or too high is not good for the improvement of the health of the pets.

[0173] In summary, the composition for improving the health of pets provided by the present application can improve the intestinal health of the pets, reduce the diarrhea rate, and additionally improve the antioxidant capacity and immune capacity of the pets, and improve the hair health of the pets.

[0174] The above-described embodiments are merely preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Any equivalent substitutions or transformations made by those skilled in the art based on the present application are within the protection scope of the present application. The protection scope of the present application is subject to the claims.

Claims

1. A nutritional composition for improving the health of a pet, characterized in that, Consists of dietary fiber, probiotic and oil, wherein: The dietary fiber comprises blueberry powder, cranberry powder, pumpkin powder, carrot powder and chicory powder; The probiotic is at least one of Bacillus coagulans MTCC5856, Enterococcus faecalis GDMCC No:62869, Bifidobacterium longum GDMCC No:62866, Enterococcus faecium GDMCC No:62870; The oil is at least one of fish oil, animal oil and plant oil, and the oil contains polyunsaturated fatty acid omega-3 and polyunsaturated fatty acid omega-6.

2. The nutritional composition for improving the health of a pet according to claim 1, wherein The nutritional composition comprises the following components by weight parts: 6-15 parts of dietary fiber, 8-12 parts of polyunsaturated fatty acid omega-3, and 21-39 parts of polyunsaturated fatty acid omega-6.

3. The nutritional composition for improving the health of a pet according to claim 2, wherein In the nutritional composition, the addition amount of each component in the dietary fiber is 0.2-3 parts of blueberry powder, 0.2-3 parts of cranberry powder, 0.5-4 parts of pumpkin powder, 4-9 parts of carrot powder, and 0.1-2 parts of chicory powder.

4. The nutritional composition for improving the health of a pet according to claim 3, wherein In the nutritional composition, the addition amount of each component in the dietary fiber is 1 part of blueberry powder, 1 part of cranberry powder, 2 parts of pumpkin powder, 7 parts of carrot powder, and 0.5 parts of chicory powder.

5. The nutritional composition for improving the health of a pet according to claim 1, wherein In the nutritional composition, the type and addition amount of probiotic are selected from any one of the following groups: a. Bacillus coagulans MTCC 5856, added in an amount of (1-5) * 10 6 CFU / g; b. Enterococcus faecalis GDMCC No: 62869, added amount is (0.5~6.5)*10 9 CFU / g; c. Enterococcus faecalis GDMCC No: 62869, Bifidobacterium longum GDMCC No: 62866 and Enterococcus faecium GDMCC No: 62870 in a total amount of (0.4-2.5)*10 10 CFU / g.

6. The nutritional composition for improving the health of a pet according to claim 5, wherein In the nutritional composition, the type and addition amount of probiotic are selected from any one of the following groups: a. Bacillus coagulans MTCC 5856, added at 3.88*10 6 CFU / g; b. Enterococcus faecalis GDMCC No: 62869, added amount is (1.94~4.85)*10 9 CFU / g; c. Enterococcus faecalis GDMCC No: 62869, Bifidobacterium longum GDMCC No: 62866 and Enterococcus faecium GDMCC No: 62870 in a total amount of (0.58-1.46)*10 10 CFU / g.

7. The nutritional composition for improving the health of a pet according to claim 2, wherein In the nutritional composition, the ratio of polyunsaturated fatty acid omega-3 to omega-6 is 1:

3.

8. The nutritional composition for improving the health of a pet according to claim 7, wherein In the nutritional composition, the content of polyunsaturated fatty acid omega-3 is 10 parts, and the content of polyunsaturated fatty acid omega-6 is 30 parts.

9. Use of the nutritional composition of any one of claims 1-8 in the preparation of pet food.

10. A pet food, characterized by, The pet food comprises the nutritional composition of any one of claims 1-8.

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