Composite reagent capable of significantly promoting germination of epimedium seeds

Abstract

Disclosed is a composite reagent capable of significantly promoting germination of Epimedium seeds, comprising the following components: molybdenum salt, alfalfa root extract, astragalus leaf extract, gibberellin, γ-aminobutyric acid, and water. The composite reagent of the present invention can greatly increase the germination rate of Epimedium seeds, and can thus expand the planting scale thereof.

Description

A compound reagent that can significantly promote the germination of Epimedium seeds Technical Field

[0001] This invention belongs to the field of agricultural technology, specifically a compound microbial agent for regulating the intestinal microbial environment of animals. Background Technology

[0002] Epimedium is a plant belonging to the genus Epimedium in the family Berberidaceae. It is used as a medicinal material.

[0003] Epimedium has pungent, sweet, and warm properties; it tonifies kidney yang, strengthens tendons and bones, and dispels wind and dampness. In recent years, with the deepening research on epimedium, its usage has increased significantly, thus requiring large-scale cultivation to meet demand.

[0004] During the ripening period of Epimedium seeds, the follicles split open, but the embryo is not fully formed, resulting in a low germination rate of the seeds under natural conditions. This is a problem for the large-scale production of Epimedium. Therefore, this invention solves the problem by providing a compound reagent that can significantly promote the germination of Epimedium seeds.

[0005] Summary of the Invention

[0006] The purpose of this invention is to provide a compound reagent that can significantly promote the germination of Epimedium seeds, thereby overcoming the shortcomings of the prior art.

[0007] The technical solution adopted in this invention is as follows:

[0008] A compound reagent that can significantly promote the germination of Epimedium seeds is made of the following components by weight percentage: 1-1.5% molybdenum salt, 4-6% alfalfa root extract, 0.3-0.6% Astragalus leaf extract, 0.3-0.6% gibberellin, 1-2% γ-aminobutyric acid, and the remainder is water.

[0009] As a further technical solution: the method for preparing the alfalfa root extract is as follows:

[0010] (1) First, peel the alfalfa roots and then dry them;

[0011] (2) The dried alfalfa roots are crushed to obtain alfalfa root powder;

[0012] (3) Mix alfalfa root powder with water at a mass ratio of 1:10, then adjust the temperature to 80℃, keep warm and stir for 4 hours, centrifuge at 5000r / min for 20min, filter, and obtain the first filtrate and filter residue.

[0013] (4) Mix the filter residue with the ethanol solution, stir at room temperature for 2 hours, centrifuge at 5500 r / min for 15 min, filter to obtain a secondary filtrate, and dry the secondary filtrate by rotary evaporation to obtain a secondary extract.

[0014] (5) Add the secondary extract to the primary filtrate, stir for 3 minutes, then ultrasonically disperse for 3 minutes, and finally concentrate to 10% of the original volume to obtain alfalfa root extract.

[0015] As a further technical solution: the power of the ultrasonic dispersion treatment is 500W;

[0016] The ethanol mass fraction is 75%;

[0017] The mass ratio of the filter residue to the ethanol solution is 1:6.

[0018] As a further technical solution: the molybdenum salt is molybdenum sulfate.

[0019] As a further technical solution: the preparation method of the Astragalus leaf extract is as follows:

[0020] Chop the astragalus leaves into small pieces;

[0021] Mix the chopped astragalus leaves with water at a mass ratio of 1:5, and then beat the mixture for 10 minutes to obtain a pulp.

[0022] Add an 80% ethanol solution to the slurry, stir for 2 hours, centrifuge at 7500 r / min for 10 min, and filter to obtain the filtrate.

[0023] The filtrate was spray-dried to obtain Astragalus leaf extract.

[0024] As a further technical solution: the mixing ratio of the slurry and the 80% ethanol solution is 1:5;

[0025] The pulping speed is 3500 r / min.

[0026] As a further technical solution: the mass ratio of Astragalus leaf extract to gibberellin is 1:1.

[0027] As a further technical solution: the composite reagent needs to be used in conjunction with irradiation.

[0028] As a further technical solution: the method of using the composite reagent is as follows:

[0029] Epimedium seeds were irradiated once, then mixed with a compound reagent at a mass ratio of 1:30-32, soaked at 5℃ for 35 hours, filtered, drained, and then subjected to a second irradiation treatment before sowing.

[0030] The single irradiation treatment is as follows: 12 C 6+ The irradiation dose was 35 Gy, and the dose rate was 60 Gy / min.

[0031] The secondary irradiation treatment is as follows: 12 C 6+ The irradiation dose was 50 Gy, and the dose rate was 40 Gy / min. Beneficial effects:

[0032] This invention's compound reagent can significantly improve the germination rate of Epimedium seeds, thereby expanding its cultivation scale. This invention primarily promotes Epimedium seed germination through irradiation treatment combined with the compound reagent. A single irradiation treatment causes slight DNA damage within the Epimedium seed, triggering its DNA repair mechanism and activating various enzymes, thus laying the foundation for subsequent seed germination. Subsequent impregnation with the compound reagent, through the penetration of active ingredients, further enhances enzyme activity, accelerates metabolism within the Epimedium seed, promotes cell division, and accelerates embryo development, further promoting seed germination and growth. Finally, a second irradiation treatment further stimulates the vigor of the Epimedium seeds and shortens germination time. Attached Figure Description

[0033] Figure 1 is a bar chart showing the germination time of 90% of Epimedium seeds. Detailed Implementation

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] The following are specific embodiments.

[0036] Example 1

[0037] A compound reagent that can significantly promote the germination of Epimedium seeds comprises the following components by weight percentage: 1% molybdenum salt, 4% alfalfa root extract, 0.3% Astragalus leaf extract, 0.3% gibberellin, 1% γ-aminobutyric acid, and the remainder being water; the alfalfa root extract is prepared as follows:

[0038] (1) First, peel the alfalfa roots and then dry them;

[0039] (2) The dried alfalfa roots are crushed to obtain alfalfa root powder;

[0040] (3) Mix alfalfa root powder with water at a mass ratio of 1:10, then adjust the temperature to 80℃, keep warm and stir for 4 hours, centrifuge at 5000r / min for 20min, filter, and obtain the first filtrate and filter residue.

[0041] (4) Mix the filter residue with 75% ethanol solution, stir at room temperature for 2 hours, centrifuge at 5500 r / min for 15 min, filter to obtain a secondary filtrate, and dry the secondary filtrate by rotary evaporation to obtain a secondary extract.

[0042] (5) Add the secondary extract to the primary filtrate, stir for 3 min, then ultrasonically disperse for 3 min, and finally concentrate to 10% of the original volume to obtain alfalfa root extract; the ultrasonic dispersion power is 500W.

[0043] The mass ratio of filter residue to ethanol solution is 1:6; the molybdenum salt is molybdenum sulfate; the preparation method of Astragalus leaf extract is as follows:

[0044] Chop the astragalus leaves into small pieces;

[0045] Mix the chopped astragalus leaves with water at a mass ratio of 1:5, and then beat the mixture for 10 minutes to obtain a pulp.

[0046] Add an 80% ethanol solution to the slurry, stir for 2 hours, centrifuge at 7500 r / min for 10 min, and filter to obtain the filtrate.

[0047] The filtrate was spray-dried to obtain Astragalus leaf extract; the mixture ratio of the slurry and 80% ethanol solution was 1:5.

[0048] The pulping speed is 3500 r / min; the mass ratio of Astragalus leaf extract to gibberellin is 1:1; the compound reagent needs to be used in conjunction with irradiation; the method of using the compound reagent is as follows:

[0049] Epimedium seeds were irradiated once, then mixed with a compound reagent at a mass ratio of 1:30, soaked at 5°C for 35 hours, filtered, drained, and then subjected to a second irradiation treatment before sowing.

[0050] One irradiation treatment is as follows: 12 C 6+ The irradiation dose was 35 Gy, and the dose rate was 60 Gy / min.

[0051] The secondary irradiation treatment is as follows: 12 C 6+ The irradiation dose was 50 Gy, and the dose rate was 40 Gy / min.

[0052] Example 2

[0053] A compound reagent that can significantly promote the germination of Epimedium seeds comprises the following components by weight percentage: 1.2% molybdenum salt, 4.5% alfalfa root extract, 0.4% Astragalus leaf extract, 0.4% gibberellin, 1.5% γ-aminobutyric acid, and the remainder being water; the alfalfa root extract is prepared as follows:

[0054] (1) First, peel the alfalfa roots and then dry them;

[0055] (2) The dried alfalfa roots are crushed to obtain alfalfa root powder;

[0056] (3) Mix alfalfa root powder with water at a mass ratio of 1:10, then adjust the temperature to 80℃, keep warm and stir for 4 hours, centrifuge at 5000r / min for 20min, filter, and obtain the first filtrate and filter residue.

[0057] (4) Mix the filter residue with 75% ethanol solution, stir at room temperature for 2 hours, centrifuge at 5500 r / min for 15 min, filter to obtain a secondary filtrate, and dry the secondary filtrate by rotary evaporation to obtain a secondary extract.

[0058] (5) Add the secondary extract to the primary filtrate, stir for 3 min, then ultrasonically disperse for 3 min, and finally concentrate to 10% of the original volume to obtain alfalfa root extract; the ultrasonic dispersion power is 500W.

[0059] The mass ratio of filter residue to ethanol solution is 1:6; the molybdenum salt is molybdenum sulfate; the preparation method of Astragalus leaf extract is as follows:

[0060] Chop the astragalus leaves into small pieces;

[0061] Mix the chopped astragalus leaves with water at a mass ratio of 1:5, and then beat the mixture for 10 minutes to obtain a pulp.

[0062] Add an 80% ethanol solution to the slurry, stir for 2 hours, centrifuge at 7500 r / min for 10 min, and filter to obtain the filtrate.

[0063] The filtrate was spray-dried to obtain Astragalus leaf extract; the mixture ratio of the slurry and 80% ethanol solution was 1:5.

[0064] The pulping speed is 3500 r / min; the mass ratio of Astragalus leaf extract to gibberellin is 1:1; the compound reagent needs to be used in conjunction with irradiation; the method of using the compound reagent is as follows:

[0065] Epimedium seeds were irradiated once, then mixed with a compound reagent at a mass ratio of 1:31, soaked at 5°C for 35 hours, filtered, drained, and then subjected to a second irradiation treatment before sowing.

[0066] One irradiation treatment is as follows: 12 C 6+ The irradiation dose was 35 Gy, and the dose rate was 60 Gy / min.

[0067] The secondary irradiation treatment is as follows: 12 C 6+ The irradiation dose was 50 Gy, and the dose rate was 40 Gy / min.

[0068] Example 3

[0069] A compound reagent that can significantly promote the germination of Epimedium seeds comprises the following components by weight percentage: 1.5% molybdenum salt, 6% alfalfa root extract, 0.6% Astragalus leaf extract, 0.6% gibberellin, 2% γ-aminobutyric acid, and the remainder being water; the alfalfa root extract is prepared as follows:

[0070] (1) First, peel the alfalfa roots and then dry them;

[0071] (2) The dried alfalfa roots are crushed to obtain alfalfa root powder;

[0072] (3) Mix alfalfa root powder with water at a mass ratio of 1:10, then adjust the temperature to 80℃, keep warm and stir for 4 hours, centrifuge at 5000r / min for 20min, filter, and obtain the first filtrate and filter residue.

[0073] (4) Mix the filter residue with 75% ethanol solution, stir at room temperature for 2 hours, centrifuge at 5500 r / min for 15 min, filter to obtain a secondary filtrate, and dry the secondary filtrate by rotary evaporation to obtain a secondary extract.

[0074] (5) Add the secondary extract to the primary filtrate, stir for 3 min, then ultrasonically disperse for 3 min, and finally concentrate to 10% of the original volume to obtain alfalfa root extract; the ultrasonic dispersion power is 500W.

[0075] The mass ratio of filter residue to ethanol solution is 1:6; the molybdenum salt is molybdenum sulfate; the preparation method of Astragalus leaf extract is as follows:

[0076] Chop the astragalus leaves into small pieces;

[0077] Mix the chopped astragalus leaves with water at a mass ratio of 1:5, and then beat the mixture for 10 minutes to obtain a pulp.

[0078] Add an 80% ethanol solution to the slurry, stir for 2 hours, centrifuge at 7500 r / min for 10 min, and filter to obtain the filtrate.

[0079] The filtrate was spray-dried to obtain Astragalus leaf extract; the mixture ratio of the slurry and 80% ethanol solution was 1:5.

[0080] The pulping speed is 3500 r / min; the mass ratio of Astragalus leaf extract to gibberellin is 1:1; the compound reagent needs to be used in conjunction with irradiation; the method of using the compound reagent is as follows:

[0081] Epimedium seeds were irradiated once, then mixed with a compound reagent at a mass ratio of 1:32, soaked at 5°C for 35 hours, filtered, drained, and then subjected to a second irradiation treatment before sowing.

[0082] One irradiation treatment is as follows: 12 C 6+ The irradiation dose was 35 Gy, and the dose rate was 60 Gy / min.

[0083] The secondary irradiation treatment is as follows: 12 C 6+ The irradiation dose was 50 Gy, and the dose rate was 40 Gy / min.

[0084] Comparative Example 1:

[0085] Based on the technical solution of Example 1, alfalfa root extract is not added, while the rest of the technical solution remains unchanged.

[0086] Comparative Example 2:

[0087] Based on the technical solution of Example 1, without adding Astragalus leaf extract, the rest of the technical solution remains unchanged.

[0088] Comparative Example 3

[0089] Based on the technical solution of Example 1, the composite reagent is not irradiated during use, while the rest of the technical solution remains unchanged.

[0090] test:

[0091] Seeds of *Epimedium sagittatum* were used as the experimental subject. The treated seeds from the examples and comparative examples were mixed with perlite at a volume ratio of 1:10 and placed in bags. 120 ml of sterile water was added, and the mixture was stirred well. After standing at room temperature for 40 minutes, excess water was removed, and the bags were sealed. Each group contained 100 seeds. Germination was defined as the radicle extending 2 mm from the seed coat. The germination rate was then calculated.

[0092] Table 1

[0093] As can be seen from Table 1, the composite reagent prepared in this invention promotes the germination of Epimedium seeds.

[0094] Statistics show that 90% of the germination times in the above experiments were:

[0095] Table 2

[0096] As can be seen from Table 2, the composite reagent of the present invention can improve the germination rate of Epimedium seeds.

[0097] The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the scope of implementation shown. Any changes made in accordance with the concept of the present invention, or equivalent embodiments modified to have equivalent changes, shall be within the protection scope of the present invention as long as they do not exceed the spirit covered by the specification.

Claims

1. A composite reagent capable of significantly promoting the germination of Epimedium seed, characterized in that, The following components are included in the percentage by weight: 1-1.5% of molybdenum salt, 4-6% of alfalfa root extract, 0.3-0.6% of astragalus leaf extract, 0.3-0.6% of gibberellin, 1-2% of gamma-aminobutyric acid, and the rest is water.

2. The combination agent of claim 1, wherein: The preparation method of the alfalfa root extract is as follows: (1) first, the alfalfa root is peeled and then dried; (2) the dried alfalfa root is crushed to obtain alfalfa root powder; (3) the alfalfa root powder is mixed with water at a mass ratio of 1:10, and then the temperature is adjusted to 80℃, and the mixture is stirred for 4 hours, then centrifuged at 5000r / min for 20min, filtered to obtain the first filtrate and residue; (4) the residue is mixed with ethanol solution, stirred at room temperature for 2 hours, centrifuged at 5500r / min for 15min, filtered to obtain the second filtrate, and the second filtrate is rotary evaporated to dryness to obtain the second extract; (5) the second extract is added to the first filtrate, stirred for 3min, then ultrasonically dispersed for 3min, and finally concentrated to 10% of the original volume to obtain the alfalfa root extract.

3. The combination agent of claim 2, wherein: The power of the ultrasonic dispersion treatment is 500W; The mass fraction of ethanol is 75%; The mass ratio of the residue to ethanol solution is 1:

6.

4. The combination agent of claim 1, wherein: The molybdenum salt is molybdenum sulfate.

5. The combination agent of claim 1, wherein: The preparation method of the astragalus leaf extract is as follows: The astragalus leaf is cut; The cut astragalus leaf is mixed with water at a mass ratio of 1:5, and then beaten for 10min to obtain a slurry; 80% ethanol solution is added to the slurry, then stirred for 2 hours, centrifuged at 7500r / min for 10min, and filtered to obtain the filtrate; The filtrate is spray dried to obtain the astragalus leaf extract.

6. The combination agent of claim 5, wherein: The mixing ratio of the slurry and 80% ethanol solution is 1:5; The beating speed is 3500r / min.

7. The combination agent of claim 1, wherein: ###0002### The mass ratio of the astragalus leaf extract to gibberellin is 1:

1.

8. The combination agent of claim 1, wherein: The composite agent needs to be used with irradiation.

9. The combination agent of claim 9, wherein: The use method of the composite agent is as follows: The epimedium seed is irradiated once, then mixed with the composite agent at a mass ratio of 1:30-32, soaked at 5℃ for 35 hours, then filtered and drained, and then irradiated again before sowing; The primary irradiation treatment is: 12 C 6+ 35 Gy at a dose rate of 60 Gy / min; The secondary irradiation treatment is: 12 C 6+ The irradiation dose is 50 Gy at a dose rate of 40 Gy / min.

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