Use of medium-chain fatty acid mixed structured lipid in amelioration of dietary-induced glucose-lipid metabolism disorder

Abstract

A medium-chain fatty acid mixed structured lipid and the use thereof in the preparation of a feed, health product or food for ameliorating a dietary-induced glucose-lipid metabolism disorder. The medium-chain fatty acid mixed structured lipid contains capric-lauric triglyceride and capric-lauric diglyceride; and a mass percentage ratio of capric acid to lauric acid in the medium-chain fatty acid mixed structured lipid is 1:1 to 1:4. Adding lauric acid-rich medium-chain fatty acid mixed glycerides to high-energy feed can ameliorate the dietary-induced glucose-lipid metabolism disorder, including but not limited to reducing serum glucose levels, improving glucose homeostasis, reducing serum low-density lipoprotein cholesterol levels, and improving the expression of glucose-lipid metabolism-related genes.

Description

Application of medium-chain fatty acid mixed structured lipids rich in lauric acid in improving diet-induced glycolipid metabolism disorder TECHNICAL FIELD

[0001] The present application relates to the technical field of functional structured lipids, in particular to the application of medium-chain fatty acid mixed structured lipids rich in lauric acid in improving diet-induced glycolipid metabolism disorder. BACKGROUND

[0002] With the improvement of living standards, people's dietary structure has changed a lot, and the proportion of high-energy diet has increased significantly, leading to excess energy intake, which in turn promotes adipocyte hypertrophy and ectopic fat deposition, resulting in glycolipid metabolism disorder. Structured lipids refer to new oils and fats that are structurally modified by changing the composition and arrangement of fatty acids on the glycerol backbone to enhance their physicochemical properties and nutritional value. Compared with natural oils and fats, structured lipids can improve the physicochemical properties of oils and fats, making them more conducive to the development and utilization of oils and fats. By synthesizing structured lipids, the content of functional fatty acids can be increased, allowing structured lipids to exert better physiological functions. Studies have shown that structured lipids have a positive impact on reducing fat accumulation and improving glycolipid metabolism.

[0003] Caprylic acid, capric acid and glycerol can be reacted to obtain caprylic acid capric acid glyceride (ODO), which has a wide range of applications in food. However, some studies have shown that caprylic acid may have an adverse effect on glycolipid metabolism, further exacerbating high-fat diet-induced blood glucose elevation and cholesterol metabolism disorder, and may also exacerbate obesity-induced changes in bone marrow microenvironment, promoting bone metastasis of cancer. SUMMARY

[0004] The present application found that medium-chain fatty acid mixed glycerides rich in lauric acid can improve diet-induced glycolipid metabolism disorder, including but not limited to reducing serum glucose levels, improving glucose homeostasis, reducing serum low-density lipoprotein cholesterol levels, and improving glycolipid metabolism-related gene expression.

[0005] Based on the discovery of the new function of medium-chain fatty acid mixed glycerides rich in lauric acid in the present application:

[0006] The first object of the present application is to provide the application of medium-chain fatty acid mixed structured lipids in the preparation of health products or food for improving diet-induced glycolipid metabolism disorder, wherein the medium-chain fatty acid mixed structured lipids contain capric acid lauric acid triglyceride and capric acid lauric acid diglyceride.

[0007] The second object of the present application is to provide the application of medium-chain fatty acid mixed structured lipids in the preparation of feed for improving glycolipid metabolism disorder induced by high-energy diet, wherein the medium-chain fatty acid mixed structured lipids contain capric acid lauric acid triglyceride and capric acid lauric acid diglyceride.

[0008] For the above two applications:

[0009] Optionally, the mass percentage ratio of capric acid and lauric acid in the mixed medium-chain fatty acid structured lipid is 1:1-1:4. Further, the mass percentage ratio of capric acid and lauric acid in the mixed medium-chain fatty acid structured lipid is 1:2-1:3.

[0010] Optionally, the capric acid lauric acid triglyceride is capric acid di-lauric acid triglyceride and dicapric acid monolauric acid triglyceride; and the capric acid lauric acid diglyceride is monocapric acid monolauric acid diglyceride.

[0011] Optionally, the capric acid di-lauric acid triglyceride is at least one of 1-capric acid-2,3-di-lauric acid glyceride and 1,3-di-lauric acid-2-capric acid glyceride;

[0012] the dicapric acid monolauric acid triglyceride is at least one of 1,2-dicapric acid-3-lauric acid glyceride and 1,3-dicapric acid-2-lauric acid glyceride;

[0013] the monocapric acid monolauric acid diglyceride is at least one of 1-capric acid-3-lauric acid diglyceride, 1-capric acid-2-lauric acid diglyceride and 2-capric acid-1-lauric acid diglyceride.

[0014] Optionally, the mixed medium-chain fatty acid structured lipid contains, in mass percentage:

[0015] the capric acid di-lauric acid triglyceride is not less than 30%;

[0016] the dicapric acid monolauric acid triglyceride is 15-30%;

[0017] the monocapric acid monolauric acid diglyceride is 20-25%.

[0018] Optionally, the improvement of the sugar-fat metabolism disorder induced by diet includes at least one of lowering serum glucose level, improving sugar homeostasis, lowering serum low-density lipoprotein cholesterol level and improving expression of sugar-fat metabolism related genes.

[0019] For its application in the preparation of health products or food:

[0020] Optionally, the mixed medium-chain fatty acid structured lipid can be directly added to food or prepared into soft capsules as a dietary supplement or health product, and the recommended daily intake is 0.25g-2.30g; more preferably, the recommended daily intake of the mixed medium-chain fatty acid structured lipid is 0.6g-1.50g; most preferably, the recommended daily intake of the mixed medium-chain fatty acid structured lipid is 0.8g-1.2g.

[0021] For its application as a feed additive:

[0022] Optionally, the medium-chain fatty acid mixed structured lipid is added to the high-energy feed at 100-3000 mg / kg.

[0023] Further, the medium-chain fatty acid mixed structured lipid is added to the high-energy feed at 300-2500 mg / kg; more preferably, the medium-chain fatty acid mixed structured lipid is added to the high-energy feed at 800-1500 mg / kg; most preferably, the medium-chain fatty acid mixed structured lipid is added to the high-energy feed at 1000-1200 mg / kg.

[0024] Optionally, the high-energy feed generally refers to high-fat feed, high-sugar feed or high-fat high-sugar feed, such as feed with a fat energy supply ratio content of more than 35%, a fat energy supply ratio in the range of 35-70% or feed containing 60% fructose or sucrose; further, it can be high-fat feed with a fat energy supply ratio of 45%.

[0025] The application also provides a medium-chain fatty acid mixed structured lipid, which contains capric acid lauric acid triglyceride and capric acid lauric acid diglyceride; the mass percentage content ratio of capric acid and lauric acid in the medium-chain fatty acid mixed structured lipid is 1:1-1:4. Further, the mass percentage content ratio of capric acid and lauric acid in the medium-chain fatty acid mixed structured lipid is 1:2-1:3.

[0026] The application also provides a preparation method of the medium-chain fatty acid mixed structured lipid, which comprises:

[0027] The capric acid, lauric acid and glycerol are mixed at 45-75°C, and after being fully liquefied and mixed, a fixed lipase or a chemical catalyst is added, vacuum is extracted or a molecular sieve is added, and the reaction is kept for 4-12 h; after removing the solid by filtration, the unreacted fatty acid, glycerol and monoglyceride are removed by molecular distillation or chemical separation method, and the medium-chain fatty acid mixed structured lipid is obtained.

[0028] Compared with the prior art, the application has the following beneficial effects:

[0029] The application researches and finds that the medium-chain fatty acid mixed structured lipid rich in lauric acid can significantly improve the sugar metabolism disorder induced by high-energy diet compared with the medium-chain fatty acid mixed structured lipid rich in capric acid and other similar products capric acid lauric acid glyceride, including but not limited to reducing serum glucose level, improving glucose homeostasis, reducing serum low-density lipoprotein cholesterol level and improving sugar and lipid metabolism related gene expression. BRIEF DESCRIPTION OF DRAWINGS

[0030] Fig. 1 is a result graph of the influence of different doses of capric acid lauric acid mixed structured lipid and capric acid lauric acid glyceride on sugar and lipid metabolism;

[0031] Figure 2 is a graph showing the effect of lauric acid and capric acid mixed structured lipids on glucose metabolism;

[0032] Figure 3 is a graph showing the effect of lauric acid and capric acid mixed structured lipids on serum lipid metabolism related indicators;

[0033] Figure 4 is a graph showing the effect of lauric acid and capric acid mixed structured lipids on the expression of liver glycolipid metabolism related genes. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the description of the present application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application.

[0036] The present application provides a lauric acid-rich lauric acid and capric acid mixed structured lipid, which comprises capric acid-dilaurylglycerol, dicapric acid-monolaurylglycerol and capric acid-monolaurylglycerol diester, and the mass percentage ratio of capric acid and lauric acid in the lauric acid and capric acid mixed structured lipid is 1:1-1:4, and further preferably 1:2-1:4.

[0037] The capric acid-dilaurylglycerol is at least one of 1-capric acid-2,3-dilaurylglycerol and 2-capric acid-1,3-dilaurylglycerol; the dicapric acid-monolaurylglycerol is at least one of 1,2-dicapric acid-3-laurylglycerol and 1,3-dicapric acid-2-laurylglycerol; and the capric acid-monolaurylglycerol diester comprises at least one of 1-capric acid-3-laurylglycerol diester, 1-capric acid-2-laurylglycerol diester and 2-capric acid-1-laurylglycerol diester.

[0038] A method for synthesizing the above mixed structured lipid comprises: esterification of capric acid, lauric acid and glycerol to synthesize the lauric acid and capric acid mixed structured lipid. The esterification refers to a process in which fatty acids and alcohols react under the catalysis of acid, base or enzyme to generate ester compounds.

[0039] In the esterification, the mixed molar ratio of capric acid, lauric acid and glycerol is 1:(1-8):1. Preferably, the mixed molar ratio is 1:(2-4):1, and the mixed structured lipid synthesized under this condition is rich in lauric acid.

[0040] When the esterification reaction occurs, 4-12% of the immobilized lipase by mass of the substrate or 0.2-0.8% of the chemical catalyst by mass of the substrate is added. The immobilized lipase used is any one of immobilized Aspergillus oryzae lipase, Rhizopus oryzae lipase, and Rhizopus delemar lipase, and the chemical catalyst is a strong acid or a strong base.

[0041] The esterification reaction is carried out as follows: the reactants are mixed at 45-75°C, and after being fully liquefied and mixed, the immobilized lipase is added, vacuum is applied or molecular sieves are added, and the reaction is incubated for 4-10 h. After the solid is removed by filtration, molecular distillation or chemical catalysis is used to remove the fatty acids, glycerol, and monoglycerides, and the lauric acid capric acid mixed structured lipid is obtained.

[0042] The application of the above-mentioned mixed structured lipid in improving high-energy diet-induced glucose and lipid metabolism disorders. In some examples, at least one of the following functions is required: reducing serum glucose levels, improving glucose homeostasis, reducing serum low-density lipoprotein cholesterol levels, and improving at least one of the functions related to gene expression in glucose and lipid metabolism.

[0043] The lauric acid capric acid mixed structured lipid (LDL) rich in lauric acid and the capric acid lauric acid mixed structured lipid (DLD) rich in capric acid are synthesized by the above-mentioned method, triglyceride tri-capric acid and triglyceride tri-lauric acid are directly mixed according to the fatty acid molar ratio of LDL to obtain a physical mixed oil (MLD), and octodecanocapric acid glyceride (ODO) is a commercially available product. The efficacy of the mixed structured lipid in improving diet-induced glucose and lipid metabolism disorders is evaluated through animal experiments. C57BL / 6J mice are randomly divided into groups, each group has 10 mice, and they are free to drink and eat water. After 16 weeks, samples are taken. The control group is fed with ordinary feed (fat energy ratio is 10%), the high-energy group is fed with high-fat feed (fat energy ratio is 45%), experimental group 1 is fed with high-fat feed added with 300-2400 mg / kg LDL, experimental group 2 is fed with high-fat feed added with 300-2400 mg / kg DLD, experimental group 3 is fed with high-fat feed added with 300-2400 mg / kg ODO, and experimental group 4 is fed with high-fat feed added with 300-2400 mg / kg MLD.

[0044] The following is described with specific examples:

[0045] Example 1: Synthesis of capric acid lauric acid mixed structured lipid

[0046] Capric acid, lauric acid and glycerol were mixed in a molar ratio of 1:2:1, placed in a constant temperature water bath shaker at 65°C, completely melted and mixed, then 10% immobilized lipase and appropriate amount of molecular sieve were added, covered with a flip cap, reacted at 200 rpm for 6h, then filtered to remove lipase and molecular sieve. The product was dissolved in n-hexane, phenolphthalein was used as an indicator, KOH methanol solution was titrated to pink, excess fatty acid was removed, n-hexane was removed by rotary evaporation, and lauric acid-rich capric acid-lauric acid mixed structured lipid LDL was obtained. Change the ratio of capric acid and lauric acid (i.e. capric acid, lauric acid and glycerol are mixed in a molar ratio of 2:1:1), and synthesize capric acid-rich capric acid-lauric acid mixed structured lipid DLD according to the above method. An appropriate amount of the above mixed structured lipid was weighed and identified by GC-MS (gas chromatography-mass spectrometry). The composition is shown in Table 1 (LDL) and Table 2 (DLD).

[0047] Table 1 Composition of lauric acid-rich capric acid-lauric acid mixed structured lipid (LDL)

[0048] (Capric acid: lauric acid = 1:2.3)

[0049]

[0050] Table 2 Composition of capric acid-rich capric acid-lauric acid mixed structured lipid (DLD)

[0051] (Capric acid: lauric acid = 1.7:1)

[0052]

[0053] Example 2: Capric acid-lauric acid mixed structured lipid improves high-fat diet-induced glucose and lipid metabolism disorder

[0054] C57BL / 6J mice were randomly divided into groups, 10 mice per group, free drinking water, NCD group fed with ordinary feed (fat energy ratio 10%), HFD group fed with high-fat feed (fat energy ratio 45%), experimental groups added LDL (Table 1) 300, 1200 mg / kg, DLD (Table 2) 1200 mg / kg and MLD in high-fat feed, and ODO 800, 1600 mg / kg. After feeding the mice for 14 weeks, they were fasted overnight, and the glucose tolerance was determined. The fasting blood glucose was measured by tail vein blood sampling, recorded as the blood glucose at 0 min, and the glucose solution (2 g / kg body weight of glucose) was injected intraperitoneally. The blood glucose value was measured by tail vein blood sampling at 30, 60, 90, 120 min, the blood glucose curve was drawn, and the area under the curve was calculated. After 16 weeks, the mice were fasted overnight, and fresh blood was collected by orbital blood sampling. After standing for 30 min, the serum was centrifuged and stored at -80°C. The levels of serum glucose and lipid metabolism-related indicators were determined using commercially available kits.

[0055] The results of serum glycolipid metabolism related indexes of mice with intragastric administration of different doses of LDL and ODO (Figure 1) show that the effect of 1200 mg / kg LDL on improving serum glucose (Glu) (A in Figure 1), serum low density lipoprotein cholesterol (LDL-C) (D in Figure 1) and high density lipoprotein cholesterol / low density lipoprotein cholesterol ratio (HDL-C / LDL-C) (E in Figure 1) is significantly stronger than that of 300 mg / kg LDL; the effect of 1600 mg / kg ODO on reducing serum glucose Glu is significantly stronger than that of 800 mg / kg ODO, and the effect of reducing serum LDL-C is stronger than that of 800 mg / kg ODO (p=0.0846). There is no significant difference in the levels of serum total triglyceride (TG) (B in Figure 1) and serum total cholesterol (T-CHO) (C in Figure 1) of each group, and the serum TG levels of 300 mg / kg LDL and 800 mg / kg ODO are slightly higher than those of 1200 mg / kg LDL and 1600 mg / kg ODO, respectively.

[0056] From the comparison, it can be seen that:

[0057] (1) The effect of 1200 mg / kg LDL on improving glycolipid metabolism is better than that of 300 mg / kg LDL, and the effect of 1600 mg / kg ODO on improving glycolipid metabolism is better than that of 800 mg / kg ODO. Within a certain range, the effects of LDL and ODO on improving glycolipid metabolism both increase with the increase of their added amounts.

[0058] (4) The effect of 300 mg / kg LDL on improving glycolipid metabolism is close to that of 1600 mg / kg ODO.

[0059] (3) The effect of 1200 mg / kg LDL on improving glycolipid metabolism is significantly better than that of 1600 mg / kg ODO.

[0060] It is shown that the capric-lauric acid structured lipid can achieve a better effect on improving diet-induced glycolipid metabolism disorder than caprylocaproyl glycerides at a lower dose.

[0061] The results of glucose tolerance test showed that (Fig. 2, A and B), there was no significant difference in initial blood glucose among groups, and the blood glucose of high-fat diet groups was higher than that of NCD group at 30, 60, 90 min after intraperitoneal injection of glucose, and the blood glucose of each group returned to a similar level after 120 min. Statistical analysis of the area under the curve (AUC) found that the area under the curve (AUC) of each high-fat diet group was significantly higher than that of the NCD group, and DLD, ODO and MLD had no obvious effect, while the area under the curve (AUC) of LDL group was significantly lower than that of HFD group, indicating that LDL can improve the destruction of glucose homeostasis induced by high-fat diet. The results of serum glucose level are shown in Fig. 2C. LDL can significantly reduce the increase of blood glucose induced by high-fat diet, and ODO (p = 0.0521) and MLD (p = 0.0744) have a trend of reducing blood glucose but not significant.

[0062] The effects of lauric acid mixed with decanoic acid on serum lipid metabolism-related indicators are shown in Fig. 3. There was no significant difference in the level of serum total triglyceride (TG) (Fig. 3, A) among groups, but the structured lipid DLD rich in decanoic acid further increased the serum TG level. High-fat diet increased the levels of serum total cholesterol (T-CHO) (Fig. 3, B) and serum low-density lipoprotein cholesterol (LDL-C) (Fig. 3, C), and LDL, DLD and MDL had no obvious effect on serum T-CHO level, while ODO had a trend of further increasing serum T-CHO (p = 0.099). MLD could reduce LDL-C to a certain extent (p = 0.068) but not significantly, while LDL significantly reduced the level of serum LDL-C. From the results of high-density lipoprotein cholesterol / low-density lipoprotein cholesterol ratio (HDL-C / LDL-C) (Fig. 3, D), DLD and ODO had no obvious improvement effect, MLD and LDL could increase serum HDL-C / LDL-C, and the effect of LDL was more significant. The above results showed that LDL had good effect on regulating serum lipid metabolism.

[0063] The effects of lauric acid-rich capric acid-lauric acid mixed structured lipids on the expression of genes related to hepatic glycolipid metabolism are shown in Figure 4. High-fat diet increased the expression of G6pc (glucose-6-phosphatase catalytic subunit gene) (B in Figure 4) and Pepck (phosphoenolpyruvate carboxykinase gene) (A in Figure 4) related to hepatic gluconeogenesis, and the expression of G6pc and Pepck in the liver of each experimental group was lower than that in the HFD group, among which LDL, ODO and MLD had significant differences, and the effect of LDL was the most significant. LDL significantly improved the decrease in the expression of Gck (hexokinase gene) (C in Figure 4) related to hepatic glycolysis induced by high-fat diet, and DLD and MLD had no obvious effect, while ODO might further reduce the expression of Gck in the liver (p = 0.099). Pparg (peroxisome proliferator-activated receptor gamma gene) (D in Figure 4) and Srebp1 (sterol regulatory element binding protein 1 gene) (E in Figure 4) are related to lipid synthesis, and LDL significantly down-regulated the expression of Pparg and Srebp1 in the liver of mice fed with high-fat diet, and DLD only reduced the expression of Pparg, and ODO and MLD had no significant effect on the expression of Pparg and Srebp1 in the liver.

[0064] In summary, compared with MLD, LDL significantly reduced the serum Glu level, improved the expression of genes related to glucose homeostasis and hepatic lipid metabolism, and had a more significant effect on the expression of genes related to cholesterol metabolism and hepatic glucose metabolism, indicating that the improvement of structured lipids on glycolipid metabolism was better than that of triglyceride physically mixed oil. Compared with DLD, LDL significantly reduced the serum Glu level, improved glucose homeostasis and cholesterol metabolism, and had a more significant effect on the expression of genes related to hepatic glycolipid metabolism, indicating that lauric acid-rich capric acid-lauric acid structured lipids had a better effect on the improvement of glycolipid metabolism than capric acid-rich capric acid-lauric acid structured lipids.

[0065] Compared with 1600 mg / kg of ODO, LDL had a lower addition amount (1200 mg / kg) and could significantly reduce the serum Glu level, improve glucose homeostasis, cholesterol metabolism and the expression of genes related to hepatic lipid metabolism, and had a more significant effect on the expression of genes related to hepatic glucose metabolism, indicating that capric acid-lauric acid structured lipids could achieve a better effect on the improvement of diet-induced glycolipid metabolism disorder at a lower dose than capric acid-lauric acid structured lipids.

[0066] The above results show that lauric acid-rich capric acid-lauric acid mixed structured lipids can improve glucose homeostasis, regulate cholesterol metabolism, reduce hepatic lipid synthesis, and improve high-fat diet-induced glycolipid metabolism disorder. It is expected to be applied in food or health products for improving glycolipid metabolism disorder.

[0067] The above-described embodiments are merely illustrative of several embodiments of the present application, which are described in more detail and in a specific and detailed manner, but should not be construed as limiting the scope of the patent. It should be noted that for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present application, and these are all within the scope of the present application. Therefore, the scope of protection of the patent of the present application should be subject to the appended claims.

Claims

1. A medium-chain fatty acid mixed structured lipid, characterized by, The medium-chain fatty acid mixed structured lipid contains capric acid lauric acid triglyceride and capric acid lauric acid diglyceride; the mass percentage ratio of capric acid and lauric acid in the medium-chain fatty acid mixed structured lipid is 1:1-1:4; The capric acid lauric acid triglyceride is capric acid di-lauric acid triglyceride and dicapric acid mono-lauric acid triglyceride; the capric acid lauric acid diglyceride is capric acid mono-lauric acid diglyceride; The medium-chain fatty acid mixed structured lipid contains, in mass percentage: The capric acid di-lauric acid triglyceride is not less than 30%; The dicapric acid mono-lauric acid triglyceride is 15-30%; The capric acid mono-lauric acid diglyceride is 20-25%; The preparation of the medium-chain fatty acid mixed structured lipid comprises: Mixing capric acid, lauric acid and glycerol at 45-75°C, after being fully liquefied and mixed, adding immobilized lipase or chemical catalyst, vacuumizing or adding molecular sieve, keeping warm for 4-12h, after removing solid by filtration, removing unreacted fatty acid, glycerol and monoglyceride by molecular distillation or chemical method, the medium-chain fatty acid mixed structured lipid is obtained.

2. The medium-chain fatty acid mixed structured lipid according to claim 1, wherein, The capric acid di-lauric acid triglyceride is at least one of 1-capric acid-2, 3-di-lauric acid glycerol ester and 1, 3-di-lauric acid-2-capric acid glycerol ester; The dicapric acid mono-lauric acid triglyceride is at least one of 1, 2-dicapric acid-3-lauric acid glycerol ester and 1, 3-dicapric acid-2-lauric acid glycerol ester; The capric acid mono-lauric acid diglyceride is at least one of 1-capric acid-3-lauric acid diglyceride, 1-capric acid-2-lauric acid diglyceride and 2-capric acid-1-lauric acid diglyceride.

3. Use of the medium-chain fatty acid mixed structured lipid according to claim 1 or 2 in the preparation of feed for improving diet-induced glucose and lipid metabolism disorder.

4. Use according to claim 3, characterized in that, The improvement of diet-induced glucose and lipid metabolism disorder comprises at least one of reducing serum glucose level, improving glucose homeostasis, reducing serum low-density lipoprotein cholesterol level and improving expression of glucose and lipid metabolism related genes.

5. Use according to claim 3, characterized in that, The medium-chain fatty acid mixed structured lipid is added to high-energy feed at 100-3000mg / kg.

6. Use of the medium-chain fatty acid mixed structured lipid according to claim 1 or 2 in the preparation of health care products or food.

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