Functional skin toner

Abstract

Provided is a functional skin toner comprising Acanthopanax extract and organic vanadium as active ingredients, wherein the organic vanadium is a chelate compound of vanadium and organic acid or amino acid.

Description

functional lotion

[0001] The present invention relates to a functional cosmetic water, and more specifically, to a functional cosmetic water having excellent antioxidant effects.

[0002] The skin is a membrane covering the outside of the body that performs various physiological functions to protect the body from environmental factors such as external stimuli, disturbances, and dryness, thereby playing a role in protecting and regulating internal organs and other internal systems.

[0003] However, as age increases, the body's physiological functions decline; this leads to a decrease in the secretion of various hormones that regulate metabolism, as well as a decline in the function and activity of immune cells. Furthermore, repeated exposure to light or heat alters the appearance or function of the skin. Consequently, an increase in free radicals and reactive oxygen species results in excessive physical and chemical irritation and stress. This damages the skin by causing a decline in normal skin function, the formation of blemishes and freckles due to melanin deposition, and the acceleration of skin aging.

[0004] Efforts are continuously being made to maintain skin health by preventing such skin aging and damage and maintaining healthier, more beautiful skin, by incorporating physiologically active substances obtained from various animals, plants, and microorganisms into cosmetics to preserve the skin's inherent functions, activate skin cells, and promote skin metabolism; accordingly, cosmetics and topical skin preparations are being developed.

[0005] However, due to safety concerns such as irritation, erythema, and redness when applied to the skin, the usage of these substances is limited, or their effects are minimal, making it difficult to expect any substantial improvement in skin function.

[0006] Furthermore, while there are ongoing attempts to develop cosmetic lotions containing ingredients harmless to the human body that possess antioxidant effects capable of eliminating free radicals and reactive oxygen species, products with antioxidant effects generally exhibit cytotoxicity; therefore, there is a demand for the development of cosmetic lotions that can achieve anti-aging through antioxidant effects without cytotoxicity.

[0007] One of the various objectives of the present invention is to provide a cosmetic water that is effective for anti-aging by inhibiting oxidative stress through excellent antioxidant effects.

[0008] According to one aspect, a functional cosmetic water is provided that comprises Acanthopanax extract and organic vanadium as active ingredients, wherein the organic vanadium is a chelate compound of vanadium and an organic acid or amino acid.

[0009] In one embodiment, the above Acanthopanax extract may be included in 1.0 to 10.0 mg / ml, and the above organic vanadium may be included in 100 μg / ml or less (except for 0 μg / ml).

[0010] In one embodiment, the organic acid may be one or more selected from phosphoric acid, fumaric acid, lactic acid, citric acid, malic acid, butyric acid, formic acid, acetic acid, gluconic acid, succinic acid, tartaric acid, glutamic acid, oxalic acid, and ascorbic acid.

[0011] In one embodiment, the amino acid may be one or more selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

[0012] A functional cosmetic water according to one aspect of the present invention can exhibit an anti-aging effect on the skin by inhibiting oxidative stress through its excellent antioxidant effect.

[0013] The effects of one aspect of the present invention are not limited to the effects described above, but include all effects that can be inferred from the configurations described in the detailed description of the invention or the claims of this specification.

[0014] Figure 1 is a graph comparing cell growth efficiency according to the concentration of Acanthopanax extract.

[0015] Figure 2 is a graph comparing cell growth efficiency according to organic vanadium concentration while the concentration of Acanthopanax extract is fixed.

[0016] Figure 3 is a graph measuring the antioxidant effect according to the presence of Acanthopanax extract and organic vanadium.

[0017] Figure 4 shows the results of measuring DPPH radical scavenging activity according to whether Acanthopanax extract and organic vanadium are included.

[0018] Figure 5 shows ABTS according to the presence or absence of Acanthopanax extract and organic vanadium. +This is the result of measuring radical scavenging ability.

[0019] Figure 6 shows the measurement results of the degree of DNA damage according to the presence of Acanthopanax extract and organic vanadium in the presence of oxidative stress.

[0020] Figure 7 shows the measurement results of cell suicide-related enzyme activity according to the presence of Acanthopanax extract and organic vanadium in the presence of oxidative stress.

[0021] Figure 8 is a comparison photo of the sensory evaluation before and after.

[0022] The advantages and features of the present invention and the methods for achieving them will become clear by referring to the embodiments described in detail below together with the accompanying drawings.

[0023] However, the present invention is not limited to the embodiments disclosed below but may be implemented in various different forms. These embodiments are provided merely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention, and the present invention is defined only by the scope of the claims.

[0024] Accordingly, in some embodiments, well-known process steps, well-known structures, and well-known techniques are not specifically described to avoid the present invention being interpreted ambiguously.

[0025] The terms used herein are for describing embodiments and are not intended to limit the invention. In this specification, the singular form includes the plural form unless specifically stated otherwise.

[0026] Throughout this specification, when a part is described as 'comprising' a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0027] A functional cosmetic water, which is one aspect of the present invention, will be described in detail below.

[0028] A functional cosmetic water according to one aspect of the present invention comprises Acanthopanax extract and organic vanadium as active ingredients, wherein the organic vanadium is a chelate compound of vanadium and an organic acid or amino acid.

[0029] As used in this specification, 'Ogaphi' refers to a general term for plants of the genus Acanthopanax, meaning one of Acanthopanax, Acanthopanax senticosus, Acanthopanax senticosus var. grandiflora, Acanthopanax senticosus var. seoulensis, Acanthopanax senticosus var. insularis, Acanthopanax senticosus var. pilosa

[0030] Acanthopanax extract is derived from the leaves of the Acanthopanax tree and has generally been used as a medicinal ingredient. The main components of this extract are seven types of triterpenoid glycosides, including Eleutherosides A, B, C, D, E, F, and G. In addition, it is rich in sugars such as glucose and galactose, large amounts of carotenoids, vitamins B1, B2, and C, and minerals. It also contains glycosides such as sterols, coumarins, lignins, flavones, and futalains. Sterols have a stimulating effect on sex hormones, while coumarins provide a significant sedative effect.

[0031] According to a paper published by the Korean Society for Life Sciences, it was confirmed that applying Acanthopanax extract to vascular endothelial cells promotes endothelial cell growth but does not affect apoptosis. Additionally, Acanthopanax extract was shown to promote the migration of vascular endothelial cells.

[0032] In addition, it is known that Acanthopanax extract helps the growth of cells other than endothelial cells, and it has been confirmed that B vitamins, vitamin C, and carotenoids have antioxidant effects.

[0033] Eleutheroside E, a representative functional substance found in Acanthopanax extract, improved swimming time by more than twofold compared to the control group's 10 minutes in mouse experiments involving forced swimming and sleep disturbance. Additionally, regarding weight changes after sleep disturbance induction, the group administered eleutheroside E showed less weight loss compared to the control group. Through this, it was confirmed that it helps alleviate oxygen stress.

[0034] Based on the antioxidant and cell growth-promoting effects of this Acanthopanax extract, the inventors applied it to a cosmetic lotion to achieve skin anti-aging.

[0035] The above Acanthopanax extract can be obtained by washing the leaves of the Acanthopanax tree with deionized water, cutting them into pieces of about 1 mm to 3 mm, drying them, adding 20,000 parts by weight of distilled water to 100 parts by weight of the dried Acanthopanax leaves, heating for 30 to 40 minutes, cooling at room temperature, centrifuging at 4,000 rpm to 8,000 rpm for about 10 minutes, and then filtering, but is not limited thereto.

[0036] In nature, vanadium does not exist in a metallic state but mostly in the form of inorganic vanadium, such as V2O5. Because this inorganic vanadium exhibits toxicity such as kidney damage and nervous system problems, the United States has set an allowable limit of approximately 0.009 mg / kg for drinking water, and in Korea, the Atmospheric Environment Conservation Act and the Occupational Safety and Health Act also set permissible concentrations.

[0037] However, organic vanadium, which is made water-soluble by organicizing inorganic vanadium, is known to have low toxicity to the human body and has antioxidant effects such as removing free radicals and active oxygen.

[0038] Therefore, after repeated experiments, the inventor confirmed that by including organic vanadium and Acanthopanax extract as active ingredients, the antioxidant effect can be maximized to obtain an anti-aging effect on the skin, and thus completed the present invention.

[0039] The functional cosmetic water of the present invention may contain 1.0 to 10.0 mg / ml of the Acanthopanax extract and 100 μg / ml or less of the organic vanadium (excluding 0 μg / ml). Preferably, the functional cosmetic water of the present invention may contain 2.0 to 8.0 mg / ml of the Acanthopanax extract and 1 to 75 μg / ml of the organic vanadium. More preferably, it may contain 2.5 to 7.5 mg / ml of the Acanthopanax extract and 2 to 60 μg / ml of the organic vanadium. Most preferably, it may contain 3 to 6 mg / ml of the Acanthopanax extract and 3 to 50 μg / ml of the organic vanadium, but is not limited thereto. When the above ranges are satisfied, the antioxidant effect of the functional cosmetic water is maximized, and since it does not exhibit cytotoxicity, the anti-aging effect can be maximized.

[0040] Organic vanadium can be produced by the following methods, but is not necessarily limited thereto.

[0041] First, inorganic vanadium powder is prepared. Here, the inorganic vanadium may be one or more selected from the group consisting of vanadium pentoxide (V2O5), vanadium trioxide (V2O3), sodium metavanadate (NaVO3), ammonium metavanadate (NH4VO3), vanadium tetrachloride (VCl4), and vanadium oxychloride (VOCl3), but is not limited thereto.

[0042] At this time, it is preferable to use inorganic vanadium with a purity of 99.0 or higher and a mesh size of 325 or higher, and in this case, it may be advantageous in terms of reactivity, but is not limited thereto.

[0043] Next, amino acids and / or organic acids are prepared and mixed with distilled water to obtain an acid solution. The temperature of the acid solution is preferably 0.5 to 1.5 N (normal concentration) and more preferably 0.8 to 1.2 N (normal concentration). If the concentration of the acid solution is excessively low, the carboxyl groups (RCOO) contained in the amino acids and / or organic acids - If the concentration of ) is low, it may be insufficient to replace the oxygen ions of inorganic vanadium, and conversely, if the concentration of the acid solution is excessively high, there may be an excessive amount of amino acids or organic acids that do not participate in the reaction.

[0044] Here, the organic acid may be one or more selected from, for example, fumaric acid, lactic acid, citric acid, malic acid, butyric acid, formic acid, acetic acid, gluconic acid, succinic acid, tartaric acid, glutamic acid, oxalic acid, and ascorbic acid, but is not limited thereto.

[0045] In addition, the amino acid may be one or more selected from the group consisting of, for example, alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine, but is not limited thereto.

[0046] Next, the acid solution and the inorganic vanadium powder are mixed in a weight ratio of 2:1 to 4:1 and reacted for at least 12 hours (preferably at least 24 hours) while stirring at a speed of 1,000 rpm to 6,000 rpm.

[0047] In this reaction, chelation is carried out as the carboxyl groups of amino acids and / or organic acids replace the oxygen ions of inorganic vanadium, and organic vanadium is formed.

[0048] Taking vanadium pentoxide among inorganic vanadium as an example, the reaction equation for the formation of organic vanadium compounds is as shown in Equation 1 below.

[0049] (Equation 1) V2O5+ 10RCOOH → V2((RCOO)2)5+ 10H +

[0050] The reaction temperature of the acid and inorganic vanadium is preferably 25 to 60°C, and more preferably 35 to 40°C. If the reaction temperature is excessively low, the reaction rate may become excessively slow, and conversely, if the reaction temperature is excessively high, denaturation of the amino acid may occur.

[0051] The reaction acidity of the above acid and inorganic vanadium may be pH 0.9 to 4.5, and preferably 1.2 to 1.8. If the reaction acidity is excessively low, the degree of dissociation of the carboxyl group is low, and the number of carboxyl groups participating in the reaction may be excessively small; conversely, if the reaction acidity is excessively high, the degree of dissociation of the carboxyl group is high, but the concentration of hydrogen ions may become excessively high.

[0052] Next, the upper layer is taken from the reaction solution of the acid solution and the inorganic vanadium powder to obtain organic vanadium.

[0053] The above functional cosmetic water may further include naturally derived substances. For example, the above-mentioned naturally derived substances include Angelica extract, avocado extract, Hydrangea extract, Althea extract, Arnica extract, Aloe extract, Apricot extract, Apricot kernel extract, Ginkgo extract, Fennel extract, Turmeric extract, Oolong tea extract, Ligusticum chuanxiong extract, Echinacea leaf extract, Scutellaria baicalensis extract, Phellodendron bark extract, Coptis japonica extract, Barley extract, Capsicum sarmentosum extract, Lamium amplexicaulum extract, Watercress extract, Orange extract, Dried seawater, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat flour, Hydrolyzed silk, Artemisia capillaris extract, Hibiscus extract, Pyracantha extract, Kiwi extract, Cinchona extract, Cucumber extract, Guanosine, Gardenia extract, Sasa borealis extract, Clara extract, Walnut extract, Grapefruit extract, Chlorella extract, Mulberry extract, Gentian extract, Black tea extract, Yeast extract, Burdock extract, Fermented rice bran extract, Rice Germ oil, Comfrey extract, Collagen, Cranberry extract, Stellaria media extract, Sage extract, Soapwort extract, Sasa borealis extract, Crataegus pinnatifida extract, Zanthoxylum piperitum extract, Shiitake mushroom extract, Rehmannia glutinosa extract, Lithospermum erythrorhizon extract, Perilla frutescens extract, Linden extract, Clematis terniflora extract, Peony extract, Acorus calamus root extract, Birch extract, Equisetum arvense extract, Seiyouki-taekis, Crataegus pinnatifida extract, Western hawthorn extract, Western elderberry extract, Western yarrow extract, Western peppermint extract, Sage extract, Malva verticillata extract, Cnidium officinale extract, Aster extract, Soybean extract, Jujube extract, Thyme extract, Tea extract, Citrus peel extract, Angelica gigas extract, Calendula extract, Prunus persica kernel extract, Spruce extract, Triosteum sinense extract, Tomato extract, Natto extract, Carrot extract, Garlic extract, Wild rose extract, Hibiscus extract, Ophiopogon japonicus extract, Parsley extract, Honey, Hamamelis extract, Korean mint extract, bisabolol, loquat extract, Tussilago farfara extract, butterbur flower stem extract, Poria cocos extract, grape extract, propolis, loofah extract, safflower extract,It may include, but is not limited to, peppermint extract, Bodhi japonica extract, hop extract, pine extract, horse chestnut extract, Amaranthium extract, Sapindus mukorossi extract, lemon balm (Melissa) extract, peach extract, cornflower extract, eucalyptus extract, Saxifrage extract, Yuzu extract, Coix lacryma-jobi extract, mugwort extract, lychee extract, lavender extract, apple extract, lettuce extract, lemon extract, Astragalus sinicus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, etc.

[0054] The present invention will be explained in more detail below through examples.

[0055] Preparation

[0056] 1. Cell preparation

[0057] Human epithelial keratinocytes (HaCaT cells) were prepared to evaluate the effects of Acanthopanax sessiliflorus extract and organic vanadium on the skin. HaCaT cell (Human Adult low Calcium High Temperature) cell lines were purchased from ATCC (Gaithersburg, MD, US) and used.

[0058] 2. Preparation of Acanthopanax extract

[0059] 200g of Acanthopanax senticosus was ground to a size passable through a 240μm sieve, 1.6L of distilled water was added, and the mixture was heated at 95°C for 4 hours to extract the product. This process was repeated once, after which the filtrate was collected and filtered (Whatman No. 3 filter paper, Whatman International Ltd., Maidstone, UK). The filtrate was freeze-dried to obtain a dried powder (20.3g) (yield: 20.3%), which was stored at 4°C. For the experiment, the powder was dissolved in physiological saline to prepare a stock solution (100 mg / ml). The prepared stock solution was stored at -20°C and diluted for use.

[0060] 3. Preparation of Organic Vanadium Aqueous Solution

[0061] Vanadium pentoxide was purified to a high purity (6N:99.9999%) as inorganic vanadium and reacted with a 1N acetic acid solution at pH 1.5 and 36°C. At this time, the acetic acid solution and vanadium pentoxide were mixed in a reaction vessel at a weight ratio of 3:1 and reacted at 3,000 rpm for 24 hours, after which the upper layer was taken to obtain organic vanadium. The organic vanadium was dissolved in purified water and filtered through a 0.22 μm filter (500 mL Bottle Top Vacuum Filter 0.22 μm, Corning, River St., Oneonta, NY, USA) to obtain an aqueous solution of organic vanadium.

[0062] Experiment Example 1 - Cell Activity Experiment (1)

[0063] Since Acanthopanax extract can inhibit cell metabolism and exhibit toxicity, potentially inducing oxidative stress or apoptosis, cytotoxicity was checked after treating HaCaT cells with Acanthopanax extract to determine the appropriate dosage.

[0064] Specifically, HaCaT cells were added to DMEM (Dulbecco modified Eagle medium, Gibco-BRL, Grand Island, NY, US) medium with 10% FBS (Fetal Bovine Serum, Hyclone, Wyman Street, Waltham, MA, US) and 10,000 U / mL Penicillin-Streptomycin (PS, Gibco-BRL), and plated in a 96-well microplate with 5×10⁶ cells per well. 5 Cells were seeded at a concentration of 1 / ml and cultured for 24 hours under conditions of 37°C and 5% CO2. Subsequently, the cultured HaCaT cells were treated with Acanthopanax sessiliflorus extract at concentrations of 1.0–5.0 ml / ml, and after 24 hours of incubation, 10 μl of CCl was added per well. After another 24 hours, the absorbance was measured at a wavelength of 450 nm using an ELISA reader. This experiment was repeated three times to obtain the mean values ​​and standard deviations, and the results are shown in Figure 1.

[0065] Since no significant results were observed at concentrations of Acanthopanax sessiliflorus extract below 1.0 mg / ml, this experiment was conducted starting from a concentration of 1.0 mg / ml. Cell activity increased with increasing concentration from 1.0 mg / ml to 3.5 mg / ml; at 3.5 mg / ml, cell activity was approximately 30% of that of the untreated control group (untreated control 100, Acanthopanax sessiliflorus extract 3.5 mg / ml treatment group 134, showing a 34% increase). Meanwhile, from a concentration of 3.5 mg / ml to 10 mg / ml, cell activity showed a non-significant increase or a constant pattern, and no cytotoxicity was observed.

[0066] Experiment Example 2 - Cell Activity Experiment (2)

[0067] Since organic vanadium can also inhibit cell metabolism and exhibit toxicity, potentially inducing oxidative stress or apoptosis, we treated HaCaT cells with Acanthopanax extract and organic vanadium to determine the appropriate amount of organic vanadium to check for cytotoxicity.

[0068] Specifically, HaCaT cells were added to DMEM (Dulbecco modified Eagle medium, Gibco-BRL, Grand Island, NY, US) medium with 10% FBS (Fetal Bovine Serum, Hyclone, Wyman Street, Waltham, MA, US) and 10,000 U / mL Penicillin-Streptomycin (PS, Gibco-BRL), and plated in a 96-well microplate with 5×10⁶ cells per well. 5 Cells were seeded at 1 / ml and cultured for 24 hours under conditions of 37°C and 5% CO2. Subsequently, the cultured HaCaT cells were treated with 3.5 mg / ml of Acanthopanax sessiliflorus extract and 0–40 μg / ml of organic vanadium. After 24 hours of incubation, 10 μl of CCl was added per well, and after 24 hours, the absorbance was measured at a wavelength of 450 nm using an ELISA reader. This experiment was repeated three times to obtain the mean value and standard deviation, and the results are shown in Figure 2.

[0069] Cell activity increased in a concentration-dependent manner up to 0–30 μg / ml of organic vanadium (100 for the untreated group with Acanthopanax extract and organic vanadium, 180 for the group treated with 3.5 mg / ml of Acanthopanax extract and 30 μg / ml of organic vanadium, showing an increase of about 80%), but at 35 μg / ml or higher of organic vanadium, cell activity showed a tendency to decrease.

[0070] Experiment Example 3 - Cell Activity Experiment (3)

[0071] To determine the effects of Acanthopanax extract and organic vanadium on oxidative stress, HaCaT cells were treated with Acanthopanax extract and / or organic vanadium, and then treated with H2O2, which is known to induce oxidative stress in skin epidermal cells as a representative reactive oxygen species (Cross CE et al, Oxygen radicals and human disease. Ann Intern. Med. 1987 ; 107(4) : 526-45.; Applegate LA et al, Two genes contribute to different extents to the heme oxygenase enzyme activity measured in cultured human skin fibroblasts and keratinocytes: implications for protection against oxidant stress. Photochem Photobiol. 1995 ; 61(3) : 285-91.), and cytotoxicity was checked.

[0072] Specifically, cultured HaCaT cells were treated with 3.5 mg / ml of Acanthopanax sessiliflorus extract and / or 30 μg / ml of organic vanadium and cultured for 24 hours. After treatment with 500 μM H2O2 (Sigma-Aldrich Co.) for 2 hours, 10 μl of CCK was added per well, and after 24 hours, the absorbance was measured at a wavelength of 450 nm using an ELISA reader. This experiment was repeated three times to obtain the mean value and standard deviation, and the results are shown in Figure 3.

[0073] The treatment group treated with 500 μM H2O2 for 2 hours showed a 50% decrease in cell activity compared to the untreated group (untreated group 100, H2O2 treated group 50), while the groups with added Acanthopanax extract and organic vanadium under 500 μM H2O2 showed an increase in cell activity of approximately 10% compared to the H2O2 treated group (untreated group 100, Acanthopanax extract added group 60, organic vanadium added group 62). Meanwhile, the groups with added Acanthopanax extract and organic vanadium showed a 20% increase in cell activity compared to the untreated group (untreated group 100, Acanthopanax extract and organic vanadium added group 120). Through this, it was confirmed that the mixture of Acanthopanax extract and organic vanadium inhibits oxidative stress caused by H2O2.

[0074] Experiment Example 4 - DPPH Radical Scavenging Activity Experiment

[0075] To determine the effects of Acanthopanax extract and organic vanadium on the scavenging activity of the free radical DPPH (2,2-diphenyl-1-picrylhydrazyl), cultured HaCaT cells were treated with 3.5 mg / ml of Acanthopanax extract and / or 30 μg / ml of organic vanadium and cultured for 24 hours. After treatment with 500 μM H2O2 (Sigma-Aldrich Co.) for 2 hours, the cells were washed three times with PBS. Subsequently, 150 μL of 0.4 mM DPPH (Sigma-Aldrich Co.) solution was added to 100 μL of the cells and reacted at 37°C for 30 minutes, after which the absorbance was measured at a wavelength of 518 nm using an ELISA reader. The absorbance value for the reactant was expressed as the antioxidant activity of each sample relative to the control group as DPPH radical scavenging activity. The experiment was repeated three times to obtain the average and standard deviation values ​​of the measurements, and the results were calculated according to Equation (1) below. The results are shown in Fig. 4 below.

[0076]

[0077] As a result of confirming DPPH radical scavenging activity, the IC50 of DPPH radical scavenging activity was measured to be 2.93 mg / mL for Acanthopanax extract and 25 μg / mL for organic vanadium. In addition, the DPPH radical scavenging activity was measured to be 60% for Acanthopanax extract (3.5 mg / ml) and 63% for organic vanadium (30 μg / ml), and 80% for a mixture of Acanthopanax extract (3.5 mg / ml) and organic vanadium (30 μg / ml), confirming that oxidative stress caused by H2O2 was significantly inhibited.

[0078] Experimental Example 4 - ABTS Radical Scavenging Activity Experiment

[0079] To determine the effect of Acanthopanax extract and organic vanadium on the scavenging activity of the free radical ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), cultured HaCaT cells were treated with 3.5 mg / ml of Acanthopanax extract and / or 30 μg / ml of organic vanadium and cultured for 24 hours, then treated with 500 μM H2O2 (Sigma-Aldrich Co.) for 2 hours and washed three times with PBS. Subsequently, 7 mM ABTS (Sigma-Aldrich Co.) and 2.45 mM potassium persulfate were mixed in a 1:1 ratio and reacted in a dark room at room temperature (28°C) for 24 hours to form radicals. Before use, the ABTS solution was diluted with phosphate buffer saline (PBS, pH 7.4) using an ELISA reader until the absorbance at 734 nm was 0.7 ± 0.02. After adjusting the concentration with 190 μL of diluted ABTS radical cation solution, 10 μL was added to each cell and reacted at room temperature for 6 minutes. The absorbance was measured at a wavelength of 734 nm using an ELISA reader, and the experiment was repeated 3 times. The mean and standard deviation of the measured values ​​were obtained and calculated according to the equation (2) below, and the results are shown in Fig. 5 below.

[0080]

[0081] As a result of confirming the ABTS radical scavenging activity, the IC50 of the ABTS radical scavenging activity was measured to be 2.04 mg / mL for Acanthopanax extract and 20 μg / mL for organic vanadium. In addition, the ABTS radical scavenging activity was measured to be 53% for Acanthopanax extract (3.5 mg / ml) and 58% for organic vanadium (30 μg / ml), and 75% for a mixture of Acanthopanax extract (3.5 mg / ml) and organic vanadium (30 μg / ml), confirming that oxidative stress caused by H2O2 was significantly inhibited.

[0082] Experimental Example 5 - Experiment on Inhibition of DNA Oxidative Damage

[0083] To investigate the effects of Acanthopanax sessiliflorus extract and organic vanadium on DNA fragmentation caused by H2O2-induced oxidative stress, HaCaT cells were placed in 60Ø dishes in DMEM (Gibco-BRL) medium supplemented with 10% FBS (Hyclone) and 10,000 U / mL PS (Gibco-BRL) at a concentration of 1 x 10⁶ 9 Cells were seeded at 1 / ml and cultured for 24 hours in a 37°C, 5% CO2 incubator. Cells were treated with 3.5 mg / ml Acanthopanax sessiliflorus extract and / or 30 μg / ml organic vanadium, respectively, and cultured for another 24 hours, after which 500 μM H2O2 (Sigma-Aldrich Co.) was added for 2 hours. Subsequently, DNA was extracted using the Qiagen mini kit (Qiagen; Max-Volmer-Strabe, Hilden, Germany) and subjected to DNA electrophoresis (electrophoresis using 1% agarose gel with ethidium bromide), followed by observation using a UV detector. The results are shown in Figure 4 below.

[0084] No fragmentation was observed in the untreated group, but DNA fragmentation was observed in the H2O2-treated group. However, fragmentation tended to be somewhat inhibited in the groups with added Acanthopanax extract and organic vanadium, and fragmentation was more inhibited in the group with added mixed solution of Acanthopanax extract and organic vanadium compared to the groups with added Acanthopanax extract and organic vanadium.

[0085] Additionally, the expression patterns of Bax and caspase-3 proteins involved in the apoptosis signal associated with DNA fragmentation were examined, and the results are shown in Figure 5 below.

[0086] In the H2O2-treated group, the expression of Bax and caspase-3 proteins was observed compared to the untreated group. In the groups with added Acanthopanax extract and organic vanadium, the expression of Bax and caspase-3 proteins was inhibited, and in the group with added mixture of Acanthopanax extract and organic vanadium, the expression of both proteins was significantly inhibited. This indicates that Acanthopanax extract and organic vanadium inhibit DNA fragmentation caused by apoptosis, thereby suppressing oxidative stress induced by H2O2.

[0087] Experimental Example 6 - Experiment on the Improvement of Skin Troubles and Skin Soothing

[0088] A mask pack was prepared using a conventional method according to the composition listed in Table 1 below.

[0089] Ingredient Content: Acanthopanax extract 3.5 mg / ml, Organic vanadium 30 μg / ml, Preservatives, colorants, and flavors in appropriate amounts, Residual purification water

[0090] To evaluate the usability of the manufactured mask pack, 15 adults aged 20 to 50 who usually experience skin itching were tested. After using the product on their faces for 15 days, they were asked to evaluate the perceived efficacy according to the evaluation criteria below using the 10-point scale in Table 2, and the average values ​​are shown in Table 3 below.

[0091] Improve No change Worsen ①②③④⑤⑥⑦⑧⑨⑩

[0092] Relieves itching, soft skin, radiant skin, reduces tightness and oiliness, increases moisture 1.331.873.932.331.871.93

[0093] Referring to Fig. 3, it can be seen that the cosmetic lotion according to the present invention not only has an effect of improving itching but also has an excellent feel when used.

[0094] Meanwhile, photographs were taken of two participants in the evaluation, showing (a) before using the mask pack and (b) after 15 days of use, as shown in FIG. 8. Referring to FIG. 8, it can be confirmed that the cosmetic lotion according to the present invention has a skin trouble improvement effect and a skin soothing effect.

[0095] Although preferred embodiments of the present invention have been described illustratively above, the scope of the present invention is not limited to such specific embodiments. Other embodiments may be proposed by adding, changing, deleting, or adding components within the scope obvious to a person skilled in the art who understands the concept of the present invention, and such embodiments should also be interpreted as falling within the scope described in the claims of the present invention.

[0096] The scope of this specification is defined by the claims set forth below, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof should be interpreted as being included within the scope of this specification.

Claims

1. A functional cosmetic water containing Acanthopanax extract and organic vanadium as active ingredients The above organic vanadium is a functional cosmetic water that is a chelate compound of vanadium and an organic acid or amino acid.

2. In Paragraph 1 A functional cosmetic water containing 1.0 to 10.0 mg / ml of the above Acanthopanax extract and 100 μg / ml or less of the above organic vanadium (except for 0 μg / ml).

3. In Paragraph 1 A functional cosmetic water in which the above organic acid is one or more selected from phosphoric acid, fumaric acid, lactic acid, citric acid, malic acid, butyric acid, formic acid, acetic acid, gluconic acid, succinic acid, tartaric acid, glutamic acid, oxalic acid, and ascorbic acid.

4. In Paragraph 1 A functional cosmetic water in which the above amino acid is one or more selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

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