Method of treating animal mixtures with keratinase production, followed by energy stabilisation of nutrients and protein concentration and activation

Abstract

A method for decomposition of keratin material, e. g. mixture of feathers, is disclosed. The method comprises treating the keratin material with Bacillus cereus KB043 strain, subsequently with sodium hydroxide and potassium hydroxide, and finally with neutralizing acid. The end product is intended for use as an amino acid fertilizer.

Description

[0001] METHOD OF TREATING ANIMAL MIXTURES WITH KERATINASE PRODUCTION, FOLLOWED BY

[0002] ENERGY STABILISATION OF NUTRIENTS AND PROTEIN CONCENTRATION AND ACTIVATION.

[0003] Technical Field

[0004] The invention relates to a method of degrading animal mixtures resulting in the production of keratinase - a keratin intermediate - for remediation and multiplication of remediation processes in soil and plants and seeds, especially for energetic stabilisation of nutrients, concentration and activation of proteins, the presence of which is multiplied many times through the action of a quantum device, thus increasing the efficiency and quality of the final product.

[0005] Background

[0006] The Slovak published patent application PP 1539-2003 describes a fertiliser additive containing organically fixed nitrogen for the production of granulated or liquid fertiliser, which consists of a mixture of untreated or granulated bovine horns and hooves with a moisture content of up to 12 wt%, containing up to 18 percent of organically fixed nitrogen of protein nature, wherein the size of the granules of treated bovine horns and hooves is from 0 to 12 mm. At the same time, the essence of said solution is a method of producing the fertiliser additive containing organic nitrogen for producing granulated or liquid fertiliser, according to which the mixture is poured into a technological container, for example a reactor. 100% potassium hydroxide (KOH) is added to the mixture in a mass ratio of at least 1 : 2 to the quantity of the mixture to be processed and 200 1 of water are poured to the contents of the process vessel. The mixture can be stirred or heated in lye bath. Once dissolved, it is neutralised by adding 50 litres of 85 % phosphoric acid. After neutralisation, it can be enriched with other nutrients such as NO3, Mg, Fe, Co, B, Mn, Zn, Cu, etc. Finally, water (H2O) is added to the solution up to a volume of 1000 litres. From the point of view of plant nutrition, the nitrogen content of the soil is the most important, although the presence of phosphorus and sulphur has a positive effect too. As it is organically bound in the soil, it is released gradually. Decomposition of albuminoid substances converts it first to ammonium (NH4+) and by further oxidation to nitrate form (NO3). Fertilisers produced so far are usually low in nutrients. The nitrogen present is in nitrate form and to a lesser extent in ammonium form. In off-root application, amide nitrogen is less necrotic to plant tissue. Combination with other forms of nitrogen allows better utilisation of this nutrient by plants.

[0007] Further known is a method for producing compost according to a Hungarian invention, patent no. 205 336 A, high in organic matter with a stimulating and alternative ameliorative effect, using an additive comprising keratin and pig manure. The production method consists in separating the liquid pig manure into solid and liquid parts by phase separation. The solid part is mixed with keratin-containing substances (hair, horn). The resulting base material contains 5-50% of keratin substance. The base material is mixed with 2-8% slaked lime, 5-10% alginite, 5-10% rhyolite tuff and 10-20% peat. The mixture obtained, taking into account its total weight, is further homogenised after the addition of 10% keratin additive. This well-mixed compost is then put in a bank. Bank moisture of 65-80% is ensured with the help of a liquid containing 5- 20% dissolved keratin. After 1-2 months, the bank is stirred. According to the Hungarian invention, patent no. 205 739 A, a base material for a slow degrading biofertiliser enriched with trace elements and with a high organic content using keratin is known. The method for making this mixture is as follows. Substances containing keratin are mixed with slaked lime. They are subjected to heat treatment in an autoclave under simultaneous pressure change and constant agitation, The hot mass is filtered and filled into storage tanks. The biofertiliser mass thus obtained is supplemented with trace elements as required. The above fertilisers with keratin additives are technologically demanding, lengthy and require some time before application; heat treatment also increases the cost of its preparation.

[0008] Summary of the Invention

[0009] The deficiencies described in the prior art are remedied by the invention as defined in the present patent claims, the gist of which is that the method for treating animal mixtures focuses on complex degradation of animal mixtures, the production of keratinase and intermediate for nutrition and remediation processes in seeds, soil and plants, the application of which will significantly affect the change in the structure of the soil stock, multiply carbon sequestration, thus avoiding soil erosion, while making meaningful use of quantum saturation in the extraquantum world of energies, such as energy stabilisation of nutrients, concentration and activation of proteins, the presence of which is multiplied many times through the action of the quantum device, thus increasing the efficiency and quality of the final product. This process is transformed into binary codes and then, through a quantum device, into extraquantum communication, the language of hexagrams, trigrams and digrams (diplets) for 3D and 2D. The developed coded language is designed to communicate the initiation of the primary degradation processes in order to multiply the subsequent intracellular revitalisation processes.

[0010] Thirty-two types of energy waves through short elastic waves make this technology unique and guaranteed intellectually sustainable. The carrier of this information is a colloidal solution that is saturated with the Bacillus cereus additive KB043, biostimulants, electromagnetically saturated aqueous solution with significant saturation of soil improvement agents, bacteriocins, mycorrhizal fungi, biorepellents (isobutyl butyrate, naphthalene, hexadecane and trans- limonene oxide, and generic host compounds, limonene, cv.s-limonene oxide and ^-myrcene), concentration of activated proteins, activated humic and fulvic acids in colloidal form, elicitors with fungicidal and bactericidal effect through stimulation of the natural defence of the organism provide increased immunity, anti-stress, increased yield, destruction of pathogens / e.g. Chitopron / , biopesticides-biopreparations, cleaning pore and leaf surface, colloidal silicone with 99.99% purity, non-selective total natural bioherbicides, lipophilic derivatives of p-benzoquinone.

[0011] An amino acid fertiliser providing a change in soil structure is obtained by processing the above defined animal mixtures, among which we can include feathers, e.g. from chickens, geese, ducks, ostriches from poultry plants, and bristles, hair from cowhide, horns and hooves, pig's trotters, fish scraps, mussels, meat and bone meal, fish meal, hair, fur, residues of hides, hair, sheep's wool, camel's wool, bristles of pigs and cattle, kangaroos, bovine, camel, kangaroo bones, bones of any poultry and pig bones (hereinafter referred to as the mixture of organic materials).

[0012] The production process is carried out in processing plants - meat processing plants, collection yards, etc., where possible also drying or grinding of organic raw materials takes place. The raw materials is transported in transport containers approved by the veterinary administration and validly disinfected by an authorised company.

[0013] In particular for epidemiological reasons, the processing of the mixture of certain organic raw materials must be carried out within 24 hours. Otherwise, it is necessary to provide a refrigerated container for the storage of raw materials. The technological process itself takes place in a special stainless steel vessel secured against concentrated alkalis, into which the weighed organic raw material mixture is placed. The reaction vessel must be adapted to the strict requirements of the technological process.

[0014] The minimum dose is 500 kg of mixed organic raw materials. Subsequently, 125 1 of the Bacillus cereus strain KBO43 and 125 kg of dextrose CeHnOe are added to the reaction vessel containing the weighed mixture.

[0015] After thorough stirring for 6 hours and enzymatic reaction, a saturated crystalline aqueous solution in a volume of 50 kg / and a quantity of 105 to 150 kg - caustic soda - E524, molar mass 39.997, at enthalpy of dissolution: delta H sol. = -1112.7 J / g, electron configuration of the sodium cation 11 Na+: ls2 2s22p6 is added by means of a quantum device, or a saturated 50 1 crystalline-colloidal solution is added by means of a quantum device, to which from 45 to 88 kg of potassium hydroxide E525, molecular weight 56.11 g / mol, is added in proportion to the weight of the organic raw material mixture. The container is closed and sealed. It is poured into 150 litres of electro-saturated aqueous solution, in proportion to the weight of the organic raw material mixture.

[0016] The decomposition of the organic raw material mixture takes about 15 hours, with occasional stirring over 21 hours, the action of bacteria, enzymes, the mass of animal waste is converted into keratinase, a semi-finished product intended for neutralisation of the solution.

[0017] Neutralisation of keratinase is carried out through alternative recipes, depending on the intended use of the final product. For this type of soil and plant nutrition we use maximum high rates (from 85% to 99.99% of the value) of concentrates, which are mixed in the following quantities into keratinase, using the following neutralisation options - up to 150 kg of 2-hydroxypropane- 1,2,3-tricarboxylic acid, 3-3-carboxy-3 hydroxypeptandic acid C6H8O7 CO3H50 (COOH, or up to 140 kg of acetasol (CH3COOH), or up to - 150 kg of methanoic acid (HCOOH); or up to - 135 kg of vitriol oil; r - up to 128 kg,- E 338 -, orthophosphoric acid H3PO4; or trihydrogen(tertaoxo)phosphate in proportion to the weight of the organic raw material mixture. - These acids can be added directly to the reaction vessel, while being mixed with specially treated and electromagnetically saturated water (110 litres) before pouring them in, and under vigorous stirring the neutralisation of the intermediate is stabilised.

[0018] The pH of the prepared mixture is then measured. The sample taken is cooled to 20°C and pH of the intermediate is measured. The required pH shall be within 8 ± 0.2. pH is adjusted by adding the above acids, sodium hydroxide a potassium hydroxide. In the final dilution stage, depending on the purpose of the finished product, it is possible to infiltrate MgSC , FeSO4.7H2O, H3BO3, MnSC , ZnSC , CuSC , (NH4)3PO4, humic acid, a biostimulant obtained by bioleaching - with dissolved Fe-Mn-Si minerals, with a wide range of bacteriocins, alcohols and organic acids:

[0019] - 2-hydroxypropanoic acid (CH3-CHOH-COOH);

[0020] - propanoic acid (C2H5COOH);

[0021] - a set of carboxylic acids CH3-(CH2)2-COOH -hexanoic, 9 octadecenoic-CH3-(CH2)7- CH=CH-(CH2)7-COOH, hexanoic acid CH3-(CH2)4-COOH, butanoic acid-CH3-(CH2)2- COOH, hypochlorous acid HC1O, (TSTHjeMovC^, Ca(NO3)2, - electromagnetically saturated aqueous solution, soil improvers, bacteriocins, mycorrhizal fungi;

[0022] - biorepellent substances (-isobutyl butyrate C8HieO2 / CID7351, naphthalene CioHs, hexadecane H3C(CH2)i4CH3 and / ra / / .s-limonene oxide CioHieO and generic host compounds, limonene C10H16, cv.s-limonene oxide CioHieO / CID6432449 and ^-myrcene C10H16), a selected mixture of activated humic and fulvic acids.

[0023] After taking a control sample, the activated solution obtained by means of the quantum encoding device, hexagrams, trigrams, gigrams and (diplets) is added to the mixture thus homogenised.

[0024] The transfer of this complex information, which is based on the chemical-physical analysis of the final product, brings a new horizon in the process of the hitherto unknown influence of quantum devices, using 32 types of energies from the extra-quantum world, to increase the quality and level of implemented nutrients, amino acids, biostimulants and biofungicides and ongoing processes, technological procedures with expected transformations of the target product. The total volume of activated solution is in the ratio of 5 1 / 1000 1 of finished product. Whether the concentrate is alkaline, acidic, neutral, double saturated or triple saturated will determine the purpose of the final product.

[0025] Posttranslational modifications of proteosynthesis allow us to realise the methionine content of keratinase. And proteosynthesis that starts with the initiator tRNA, i.e. the one that carries methionine. It binds to the small ribosomal subunit and begins to slowly traverse the mRNA molecule from the 5' end. After discovering the AUG initiation sequence it binds and translation begins.

[0026] Characterisation and definition of the essence and meaningfulness of quantum saturation as energy stabilisation of nutrients, concentration and activation of proteins, the presence of which increases many times through the action of the quantum device and thus increases the efficiency and quality of the final product. At the same time, clarification of the technological language intended for communication in the initiation of the processes of primary degradation and subsequent multiplication of intracellular revitalisation processes.

[0027] Leptons, quarks, electrons and neutrins, as the smallest particles, are structured and consist of certain wave energies. Lepton and quark represent the nodal state of short elastic waves. In a cluster of particles, each particle has a different form of energy. After the particles break up, short elastic waves line up behind each other - we observe a wave and a wave state of the same particle occurs. An extra-quantum level is created, where the whole space is filled with extraquantum waves. Each wave is discrete in its own way, as it consists of short elastic waves, with energies passing from one state to another. None of them disappear, they undergo rebirth, i.e. transformation into another species with a new mission. When energy waves - waves of energies - intertwine into a cluster, the invisible becomes visible. Each particle - wave is a carrier of a certain kind of information, a code. Depending on the combination of waves, matter with definable properties is created in the material world. The characteristics of the new object are formed at the extra-quantum level. That is, the information embedded in the extra-quantum level determines what this particle will create. The defined order of the waves is sorted into a certain order, which is very important, like in DNA. Every fragment has its place. The first wave has a special role - it carries information about the whole set and carries the basic program code. All of this gives rise to the fact that instead of the 6 known types of energy, 32 types of energy act in the real world. A method for activation and energy stabilisation of micronutrient volume, via a quantum device transformed into a binary language. The carrier of this information is the colloidal solution : 0101101001101110001000000111101001101001 0110111001101111 01101011 00100000 00110000001011100011001100110111

[0028] Humic acids 64.5

[0029] 0100100001110101 01101101 01101001 0110111001101111 0111011001100101 00100000 01101011 01111001 01110011 01100101 0110110001101001 0110111001111001 00100000 00110110001101000010111000110101

[0030] Specific gravity 1247

[0031] 01001101 01100101 011100100110111001100001 001000000110100001101101 01101111 011101000110111001101111 01110011 11000101 10100101 0010000000110001 00110010 0011010000110111

[0032] Aspartic acid 11.57

[0033] 01001011 01111001 01110011 01100101 0110110001101001 0110111001100001 00100000 01100001 01110011 0111000001100001 0111001001100001 01100111 01110101 01110011 01101111 0111011001100001 0010000000110001 00110001 0010111000110101 00110111

[0034] Treonine 0.96

[0035] 010101000111001001100101 01101111 01101110 11000011 10101101 0110111000100000 00110000001011100011100100110110

[0036] Serine 4.46

[0037] 010101000111001001100101 01101110 11000011 10101101 011011100010000000110100 001011100011010000110110

[0038] Glutamic acid 18.75

[0039] 01001011 01111001 01110011 01100101 0110110001101001 0110111001100001 00100000 01100111 0110110001110101 01110100 11000011 10100001 0110111001101111 01110110 11000011 10100001 0010000000110001 001110000010111000110111 00110101

[0040] Proline 17.61

[0041] 010100000111001001101111 0110110001101001 011011100010000000110001 00110111 001011100011011000110001

[0042] Glycine 12.71

[0043] 01000111 0110110001111001 01100011 01101001 011011100010000000110001 00110010 001011100011011100110001

[0044] Alanine 11.90

[0045] 01000001 0110110001100001 0110111001101001 011011100010000000110001 00110001 001011100011100100110000

[0046] Valine 12.43

[0047] 0101011001100001 0110110001101001 011011100010000000110001 0011001000101110 0011010000110011

[0048] The basic information is based on a complete physicochemical analysis of the final product - the sum of essential nutrients, micronutrients, amino acids, humic and fulvic acids, residues of additives to improve the quality of soil structure and foliar nutrition, bacteria, biostimulants, mycorrhizal fungi - and has been transformed by a quantum device into hexagrams, trigrams and digrams (diplets) for 3D and 2D. The carrier of this information is the colloidal solution. (Figure 1 -3 ).

[0049] The convergence of advanced technologies such as artificial intelligence and high-performance computing is revolutionising engineering in physics simulation. A key component of these methods are physically informed neutron networks that contain known physical laws or principles. Using similar technologies to Siml.ai, we simulate the physical transformations and stabilisation of our product.

Claims

PATENT CLAIMS1. Method of treating animal mixtures with keratinase production, followed by energy stabilisation of nutrients and protein concentration and activation, characterized in that 125 1 of the Bacillus cereus strain KBO43 and 125 kg of dextrose GH^Or,. are added to 500 kg of a organic raw material mixture; after stirring for 6 hours and enzymatic reaction, a saturated crystalline aqueous solution in a volume of 50 kg / 1 and a quantity of 105 to 150 kg - caustic soda - E524, molar mass 39.997, at enthalpy of dissolution: delta H sol. = -1112.7 J / g, electron configuration of the sodium cation 11 Na+: ls2 2s22p6 is added by means of a quantum device, or a saturated 50 1 crystalline-colloidal solution is added by means of a quantum device, to which from 45 to 88 kg of potassium hydroxide E525, molecular weight 56.11 g / mol, is added in proportion to the weight of the organic raw material mixture, then decomposition of the organic raw material mixture occurs in a closed reaction vessel lasting 21 hours with occasional stirring, the action of bacteria and enzymes converts the mass of animal waste into keratinase, which is neutralised by being added to 150 kg of 2-hydroxypropane-l,2,3-tricarboxylic acid, 3-3-carboxy-3 hydroxypeptandioic acid C6H8O7 CO3H50 (COOH, or to 140 kg of acetasol (CH3COOH), or to 150 kg of methanoic acid (HCOOH), or to 135 kg of vitriol oil, or to 128 kg of, - E 338, orthophosphoric acid H3PO4, or trihydric phosphoric acid in proportion to the weight of the organic material mixture, while these acids can be added directly to the reaction vessel, mixing them with specially treated and electromagnetically saturated water (110 litres) before pouring and under intensive stirring to stabilise the neutralisation of the semi-finished product, then pH of the prepared mixture is measured, where a sample is taken from the neutralised prepared mixture, cooled to 20°C and its pH is measured, the desired pH of the mixture to be prepared being in the range 8 ± 0,2, and its adjustment being effected by the addition of the above acids, sodium hydroxide or potassium hydroxide, and then a control sample is taken and the activated solution obtained by means of a quantum encoding device, hexagrams, trigrams, gigrams and (diplets) is added to the mixture thus homogenised.

2. Method of treating animal mixtures with keratinase production, subsequent energy stabilisation of nutrients and protein concentration and activation according to claim 1, characterized in that in the final dilution step, depending on the purpose of the finished product, it is possible to infiltrate MgSC , FeSO4.7H2O, H3BO3, MnSC , ZnSC , CuSO4, (NH4)3PO4, humic acid, a biostimulant obtained by bioleaching - with dissolved Fe-Mn-Si minerals, with a wide range of bacteriocins, alcohols and organic acids, such as - 2-hydroxypropanoic acid (CH3-CH0H-C00H); - propanoic acid (C2H5COOH); - a set of carboxylic acids CH3-(CH2)2-COOH -hexanoic, 9 octadecenoic - CH3- (CH2)7-CH=CH-(CH2)7-COOH, hexanoic acid CH3-(CH2)4-COOH, butanoic acid- CH3-(CH2)2-COOH, hypochlorous acid HC10, (NH4)6Mo7O24, Ca(NO3)2, - electromagnetically saturated aqueous solution, soil improvers, bacteriocins, mycorrhizal fungi; biorepellent substances (-isobutyl butyrate C8HieO2 / CID7351, naphthalene CioFh, hexadecane H3C(CH2)i4CH3 and / ra / / .s-limonene oxide CioHieO and generic host compounds, limonene C10H16, cv.s-limonene oxide CioHi6O / CID6432449 and ^-myrcene C10H16), a selected mixture of activated humic and fulvic acids.

3. Method of treating animal mixtures with keratinase production, subsequent energy stabilisation of nutrients and protein concentration and activation, characterized in that the organic mixture is formed from a mixture of feathers, e.g. the organic matter is composed of a mixture of feathers, e.g. from chickens, geese, ducks, ostriches from poultry plants, and bristles, hair from cowhide, horns and hooves, pig's trotters, fish scraps, mussels, meat and bone meal, fish meal, hair, fur, residues of hides, hair, sheep's wool, camel's wool, bristles of pigs and cattle, kangaroos, bovine, camel, kangaroo bones, bones of any poultry and pig bones.

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