Antimicrobial composition, method for its preparation and antimicrobial product

Abstract

The antimicrobial composition comprises a mixture or a reaction product of at least turmeric extract (TME), japonica extract (SJE) and Azithromycin (AZM), wherein the ratio of the mass of turmeric extract (TME) to the mass of japonica extract (SJE), mT\n.: msjE is in the range from 1 : 10 to 10: 1, and wherein the ratio of the sum of the masses of turmeric extract (TME) and japonica extract (SJE) to the mass of Azithromycin (AZM), (mTME + mSJE) : mAZM is in the range from 1 : 10 to 10: 1. The composition has an excellent antimicrobial effect. It can be embedded in products or placed on their surfaces and prevents or impedes the settling of microorganisms.

Description

[0001] P5392-PCT October 17, 2024

[0002] - 1 -

[0003] ANTIMICROBIAL COMPOSITION, METHOD FOR ITS PREPARATION AND ANTIMICROBIAL PRODUCT

[0004] FIELD OF THE INVENTION

[0005] The invention relates to an antimicrobial composition, a method for the preparation of the antimicrobial composition and a masterbatch and a product comprising the antimicrobial composition.

[0006] BACKGROUND OF THE INVENTION

[0007] Harmful or opportunistic microorganisms, frequently termed as microbes, such as mold, bacteria and fungus often exist and grow in air, in water or near water sources and air. These microbes, as well as various other particles and pollutants, may be delivered to an associated water environment. They can have a negative impact on people’s health, for example through oral intake, inhalation or contact with the skin, and they may cause various illnesses.

[0008] Microorganisms such as bacteria, algae or fungi can accumulate and multiply on object surfaces. Damp or wet surfaces are particularly suitable for this. Deposits of microorganisms or microbes, are generally undesirable. They can form layers of slime in swimming pools and ponds, for example. Such slime layers increase the risk of slipping and also act as a breeding ground for germs. Swimming pools can also have plastic linings. The surfaces of such plastic linings are preferred places for microorganisms to settle. There is also a risk that microplastics will dissolve from such plastic linings and be released into the water. This is harmful to the environment. P5392-PCT October 17, 2024

[0009] - 2 -

[0010] A significant percentage of the world's population suffers from various illnesses and diseases that are caused by microorganisms. These microorganisms are mostly harmless, but some of them are potentially harmful and some of them are always harmful to humans and environment if not reduced. These discomforts and diseases include disorders caused by contaminated water, such as stomach pain, diarrhea, chronic digestive track disorders and other related illnesses. Such ailments are frequently caused or made worse by water-borne microbes and particulates. The Water quality or air quality is critical to human comfort and health.

[0011] There is a variety of approaches to reduce or eliminate negative impacts of microbes, e.g. by filtering of water and retaining microbes and other particles using purification devices, using disinfectants like chlorine to kill or destroy the microbes in water and on surfaces of objects or by using antibiotics or antimicrobials like iodine, copper and silver to kill the microbes in or on the human body.

[0012] Water purification devices including filter membranes, sand filters and / or activated carbon, for example, can be used to remove harmful microorganisms from water. Such devices must be cleaned or regenerated frequently.

[0013] There is a variety of antibiotic and antimicrobial agents that are used particularly in the medical field. Persistent use of antibiotics or antibacterials has provoked the emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) bacteria, which render even the most effective drugs ineffectual.

[0014] Iodine or iodine solutions like Lugol’s solution and povidone iodine (PVP-I), a water-soluble combination of molecular iodine and polyvinylpyrrolidone, are well- established antiseptics. Iodine is one of the most widely accepted antimicrobials in the world as it is active against bacteria, fungi and viruses and has no known P5392-PCT October 17, 2024

[0015] - 3 - acquired resistance. The mechanisms of action for iodine can include penetration into the cell wall of a microorganism, causing blocking of the hydrogen bond which results in damage to the phospholipid cell membrane or damage to and denaturing of the proteins, nucleotides and fatty acids, leading to rapid cell death by binding to thiol and sulphydryl groups. Some antiseptics with high iodine levels may cause pain and irritation when applied to open wounds. There are also concerns regarding cytotoxicity, systemic absorption and delayed wound healing.

[0016] One of the advantages of iodine as an antimicrobial is that bacterial resistance does not develop even with extended use as it does for many antibiotics. Hospital acquired infections are a serious and persistent problem, in part because many such pathogens have developed or exhibit multidrug resistance and virulence. Six of the most common and noxious nosocomial i.e. hospital acquired pathogens are known by the acronym ESKAPE, which includes: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter.

[0017] Like iodine, various forms of copper and silver or compounds with copper and silver are also known to have broad spectrum antimicrobial properties. Copper and copper ions are used as water purifier, algaecide, fungicide and anti-fouling agent. Copper Iodide (Cui) is known to have some antiviral properties. And copper ions in copper sulfate solutions have been shown to reduce the hydrophobicity of biofilm, i.e. the water repelling nature of biofilm's outer layer, which can allow greater access to companion antimicrobials to the interior microbes within a biofilm. Although copper is essential for the cells in a human body in very small quantities, excess copper levels in the body can be toxic. In general microorganisms are far more susceptible to copper relative to the lower sensitivity of human tissue. Copper is released into the water and it is not easy to control a suitable release rate. P5392-PCT October 17, 2024

[0018] - 4 -

[0019] Copper toxicity to micro-organisms including viruses, is thought to occur as a result of numerous mechanisms. Such mechanisms can include, for example, (1) the displacement of essential metals from their native binding sites; (2) interference with oxidative phosphorylation and osmotic balance; and (3) inducing alterations in the conformational structure of the nucleic acids, membranes and proteins of microbes. There has been a strong drive from scientific and commercial and governmental agencies to upgrade silver, copper and other toxic metals antimicrobials with more eco- and human friendly versions of antimicrobials.

[0020] The use of antimicrobials, and especially iodine, typically requires carefully balancing an antimicrobial's effectiveness against the risks of its toxicity to living tissue. Antimicrobial efficacy is not useful, beneficial or acceptable if it comes at the expense of damaging healthy tissue or interfering with or delaying the processes of wound healing. Most iodine-based formulations have difficulty achieving the appropriate balance. Copper, while more differentially damaging to microbes than human tissue at low concentrations, must also maintain this balance. With respect to iodine, formulations such as Betadine®'s PVP-I are not approved for use on wounds or non-intact skin. If medical professionals choose to use them on open wounds or surgical sites by diluting them, applying them and then flushing them out quickly in an attempt to avoid the toxic side effects, they engage in such off-label use a risk, as the procedure is not based on scientific evidence and the side effects are risky and unknown.

[0021] As iodine is considered one of the most efficacious broad spectrum topical antiseptics, with no known resistance, it would represent a significant breakthrough to find or develop the formulation of a composition that releases sufficiently low levels of iodine so as to not cause any delay in wound healing or result in any toxicity to healthy tissues even under prolonged contact, while still proving highly effective against infection, pathogens and biofilms with sustained antimicrobial activity, all in a solution that demonstrates the long-term stability required of an effective medical product and a bio-compatible pH. Such a formulation, further P5392-PCT October 17, 2024

[0022] - 5 - leveraged by the antimicrobial and anti-biofilm characteristics of appropriate copper complexes would represent a significant advancement in the standard of care and treatment of wounds and infection.

[0023] Such a composition that is non-toxic, safe and non-irritating to human skin and tissue, (including under prolonged contact) that is simultaneously highly effective at destroying or inactivating viruses such as coronaviruses would further provide an extremely useful and effective antimicrobial disinfectant for treating surfaces such as personal protective equipment (PPE), gloves, masks and / or skin and eliminating coronaviruses from such surfaces, one that could be used in situ as often and as frequently as necessary without causing any insult or irritation to surrounding skin and tissues.

[0024] Treatments with such antimicrobial substances must be repeated to achieve a lasting effect. These substances are released into the environment in an uncontrolled manner.

[0025] It is also state of the art to implement antimicrobial agents into Polymers.

[0026] WO20 18 / 065773 Al discloses a masterbatch for mixing with a raw polymer material during plastics manufacturing, the masterbatch comprising a thermoplastic polymer material and silver ions as an antimicrobial agent. The antimicrobial agent is dispersed within the thermoplastic polymer material, wherein the antimicrobial agent is present at a level of 20% to 30% by weight of the thermoplastic polymer material. An antimicrobial plastics product, especially a door handle may be manufactured by moulding a mixture comprising the masterbatch and a raw polymer material, wherein the masterbatch is present at a level of 1% to 5% by weight of the mixture.

[0027] An object of the present invention is to provide an antimicrobial composition with high efficiency, a product or semi-finished product comprising this antimicrobial composition and a process for manufacturing the antimicrobial composition. P5392-PCT October 17, 2024

[0028] - 6 -

[0029] A further object of the present invention is to provide the antimicrobial composition, the semi-finished product or the product in such way that they have a sustainable antibacterial effect.

[0030] These problems are addressed by an antimicrobial composition according to the features of claim 1, by a masterbatch according to claim 7, by a product or semifinished product according to the features of claim 8 and by a method according to the features of claim 10.

[0031] SUMMARY OF THE INVENTION

[0032] The formulation of a highly effective antimicrobial composition is based on antimicrobial agents including turmeric extract (TME) and stephania japonica extract (SJE) which are preferably of natural origin, in combination with azithromycin (AZM) which is known as an antibiotic. Surprisingly, the antimicrobial effect of the agents in combination is better than that of the individual agents.

[0033] The ratio of the mass of turmeric extract (TME) to the mass of japonica extract (SJE), ITITME: msjE is usually in the range from 1 : 10 to 10: 1. The ratio of the sum of the masses of turmeric extract (TME) and japonica extract (SJE) to the mass of Azithromycin (AZM), (HITME + ms®) : ITIAZM is usually in the range from 1 : 10 to 10:1.

[0034] The antimicrobial composition is a mixture or reaction mixture or a reaction product of the three basic components and preferably further components. Such further components may in particular be auxiliary substances or additives which interact with other substances in the composition. Preferably, the composition comprises nano titanium dioxide (n-TiCE) and / or N-Trimethoxysilylpropyl-N,N,N- trimethylammonium chloride. P5392-PCT October 17, 2024

[0035] - 7 -

[0036] Reaction mixtures are mixtures in which components of a mixture at least partially interact or react with each other. This can change chemical and / or physical properties such as composition or binding forces between components of the mixture. In this patent application the expression “mixture” is to be understood as also including “reaction mixtures”.

[0037] Nano titanium dioxide (n-TiCh) is not soluble in water. It remains in the reaction mixture or reaction product respectively, complexes at least partially with the other components and causes a stronger bonding of these components. It increases the wash-out resistance of the components in water. Parts of n-TiCh in the composition that do not react with other components of the mixture, retain their mild antimicrobial properties and boost the performance of the compound.

[0038] N-Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride on its own has mild antimicrobial properties. It has methoxy groups that interact with hydroxy (-OH) groups from other components in the mixture, increasing the molecular size of the compounds. Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride may attach to n-TiCh, in particular to n-TiCh, which complexes with TME, SJE, AZE, and further stimulates the formation of larger molecules or particles. This results in better anchoring of the components, in particular TME, SJE and AZE, and makes the composition or end product washing proof. This does not affect the antimicrobial effect of TME, SJE and AZE but rather improves it.

[0039] The composition may further comprise one or more of the following components: Low molecular weight polyethylene glycol (PEO)

[0040] Its main function is to offer dispersing capability so n-TiCE and other ingredients disperse into smallest possible physical sizes and leads to more uniform distribution throughout the mixture, the reaction mixture or the reaction products. The end capped -OH moi eties from this low molecular weight substance PEO will serve as functional groups participating in reaction and / or via hydrogen bonding by P5392-PCT October 17, 2024

[0041] - 8 - complexing in the reaction mixture, holding other molecules together during functioning of the reaction product, reinforcing washing off resistance.

[0042] Polyacrylic acid, aqueous suspension (PAA)

[0043] It provides hydrogen-bonding or anchoring sites for n-TiO?. It is used as a dispersant or stabilizer to promote a homogeneous distribution of the other components.

[0044] Guanidine hydrochloride (GHC1)

[0045] It can act as a complexing agent similar to n-TiCh. Its ability to denature biomolecules such as proteins and nucleic acids also has at least partial effect on the antimicrobial composition.

[0046] Hexamethylenediamine (HDMA)

[0047] It acts as crosslinker for the components of the antimicrobial composition.

[0048] Preferably, the antimicrobial composition, i.e. its components or reaction mixture or reaction product are embedded in or arranged on at least one surface section of a carrier material. Preferably, the carrier material is made of plastic or synthetic resin. It may in particular comprise or consist of at least one of a polyolefin, a polyester or a polyamide.

[0049] The compound of the carrier material and the antimicrobial composition may be a product or a semi-finished product with antimicrobial properties or an additive which can be used to produce such products or semi-finished products. In this patent application the term “product” also includes such semi-finished products and additives.

[0050] The carrier material and other components like n-TiCh and Trimethoxysilylpropyl- N,N,N-trimethylammonium chloride act alone or in combination with each other as P5392-PCT October 17, 2024

[0051] - 9 - additional antimicrobials as well as binders which hold the active substances together.

[0052] Some examples of antimicrobial compositions or additives, semi-finished products and products are given below.

[0053] In some embodiments, the antimicrobial composition comprises only a powdered mixture of SJE, TME and AZM. Preferably, this mixture also comprises n-TiCh. The preferred weight ratios of the components are as follows: IHTME: msjE : 1 : 10 to 10: 1, preferably 1 :3 to 3: 1 or 1 :2 to 2: 1; (IHTME + msis) : IUAZM : 1 : 10 to 10: 1, preferably 1 : 1 to 6: 1, in particular about 9:2; (IHTME + msis) : mTio2 : 1 :4 to 4: 1, preferably 1 : 1 to 3:1, in particular about 4:3. These specifications apply with tolerance ranges of + / - 15%.

[0054] In some embodiments, such mixtures are compressed into tablets, preferably together with a binder or carrier material.

[0055] Antimicrobial masterbatch compositions:

[0056] Masterbatches are additives that are used in the manufacture of products to give them special properties. This applies in particular to the manufacture of plastic products or resins for coating surfaces. The proportion or concentration of active ingredients like an antimicrobial composition in masterbatches is generally significantly higher than in the products to be manufactured. In the manufacture of such products, the masterbatch is added to a specific base material in a predetermined weight or volume ratio to give the product the desired properties.

[0057] The antimicrobial composition and products comprising the antimicrobial composition are compatible with foodstuffs. P5392-PCT October 17, 2024

[0058] - 10 -

[0059] In some embodiments, the masterbatch may comprise plastic pellets or granules including the antimicrobial composition. Such masterbatches can be used, for example, in the manufacture of plastic parts, particularly in the manufacture of plastic parts by injection molding or extrusion. The antimicrobial composition is distributed over the entire volume of such parts. Such antimicrobial products can be manufactured easily and efficiently, even in large quantities. Internal and external surfaces can be reliably antimicrobial treated, even for products with complex structures.

[0060] Some examples are parts of water pumps, valves, fans, water and ventilation ducts, pipes, hoses, sealing elements, parts of washing machines, tumble dryers, dishwashers, refrigerators, coffee machines, etc. in households and commercial applications, containers, pails, jars, medical apparatus, cable jacketing etc.

[0061] In some embodiments, the masterbatch may comprise a synthetic resin including the antimicrobial composition. Synthetic resins can be soft solids or highly viscous substances, for example, which usually contain prepolymers with reactive functional groups. In paints and adhesives, such synthetic resins can be used as an intermediate stage in the production of duroplastics. Thermoplastic resins or thermoplastic polymers are not curable. They can soften or melt when heat is applied and solidify again when cooled. Viscous masterbatches can be mixed with other viscous materials such as synthetic resin paints in the desired concentration. In this form, synthetic resins comprising the antimicrobial composition can be applied to the surfaces of objects. After drying and / or curing, they form an antimicrobial layer on the surfaces of these objects. This allows antimicrobial substances to be specifically placed only on selected surfaces of objects, i.e. where they are actually required. Since only the surfaces of objects are coated, the material consumption is low compared to the fullvolume antimicrobial treatment of objects. This applies even if the surfaces are coated several times, e.g. about two to ten times, in particular three to six times.

[0062] Once the antimicrobial resins have solidified, they provide a sustainable, washable P5392-PCT October 17, 2024

[0063] - 11 - and waterproof coating. Such coatings can be applied easily to new and existing structures. They can be applied at low cost and are efficient and effective.

[0064] Some examples of objects of which one or more surfaces may be coated with an antimicrobial layer are listed hereafter: swimming pools or parts thereof, e.g. its walls, parts of washing machines, tumblers, refrigerators, dishwashers, water containers, water and ventilation ducts in appliances and buildings, filter devices for purification of water and / or air, sanitary building installation, water treatment systems for drinking water and / or waste water, surfaces in kitchens and / or food processing plants, surfaces in hospitals etc. Antimicrobial thin films or coatings can also be referred to as two-dimensional antimicrobial objects.

[0065] The antimicrobial composition can also be used as an additive in an impregnating agent for treating porous materials such as textiles, fleece, paper, wood or concrete, for example. The antimicrobial composition is combined with a solvent or a dispersion medium. After evaporation or drying of the solvent or dispersion medium the applied active substances provide an antimicrobial effect to the treated materials. Possible applications include filtration devices, fabrics, towels, facial masks, items of clothing such as socks or underwear, gloves, sportswear, shoes or parts thereof etc.

[0066] In other applications, antimicrobial compositions can also be combined with zeolites and / or activated carbon, for example. This is particularly useful for water treatment devices.

[0067] Once applied, the antimicrobial composition inhibits growth of microorganisms, in particular harmful microorganisms. This helps to prevent or impede the spread of diseases and infections and / or reduce malodors caused by microorganisms. P5392-PCT October 17, 2024

[0068] - 12 -

[0069] DESCRIPTION OF PROCESSES FOR THE PRODUCTION OF PREFERRED EMBODIMENTS

[0070] The following is an example of a process for producing an antimicrobial composition. The information is provided for better understanding and is not to be understood as a restriction for manufacturing processes and / or antimicrobial compositions and antimicrobial products. The quantities and procedures indicated refer to the production of small quantities in laboratories. This information can of course be scaled up for the industrial production of larger quantities. The weights refer to the reactants of the reaction mixture, which can at least partially interact or react with each other during further processing. Unless otherwise stated, weights and ratios are to be understood with a tolerance range of + / - 15%.

[0071] - Grinding and mixing of SJE (300g), TME (150g), AZM (100g) and n-TiCE (450g) for 20 minutes at 1000 rpm, preferably with intermittent stops to avoid unnecessary temperature rises over e.g. 60°C to 80°C.

[0072] - Adding PAA (1200g, 30% solid) and PEO (50g) and mixing for 5 minutes at 50 rpm to obtain a suspension.

[0073] - Drying of the mixture (e.g. for 5 hours at 110°C with reduced pressure in vacuum oven, subsequent for 1 hour at 150 °C; then let cool down to room temperature).

[0074] - Washing the resultant solid (e.g. three times with 5000 ml deionized water).

[0075] - Grinding of the solid mixture e.g. for 20 minutes at 2000 rpm.

[0076] - Drying of the resultant solid (also referred to as 2-n-TiO2) preferably for 3 hours at 150°C in a vacuum oven at reduced pressure and cool down to room temperature and store in an air-tight container. P5392-PCT October 17, 2024

[0077] - 13 -

[0078] Optional further processing steps:

[0079] - Charge GHC. (150g), HDMA (250g) and deionized water (5000ml) in an autoclave with protective nitrogen flowing in at 20ml / min. Let the gas outlet pass to a hydrochloric acid (5%) reservoir to accept NH3 gas evolved.

[0080] - Low speed stirring for 3 hours at 80°C, then charging 2-n-TiO2 (1000g) and medium speed stirring of about 50rpm, reduce pressure and rise autoclave temperature from room temperature to 130°C in about 1 hour, first 30 minutes with nitrogen gas flowing in and in the second 30 minutes time period, with nitrogen gas flow stopped. Keeping autoclave at reduced pressure, until water is basically distilled off

[0081] - Maintaining the autoclave temperature at 130°C for another 3 hours, then raise temperature to 175°C for 6 hours under vacuum.

[0082] - Rising the autoclave temperature to 195°C and maintaining for 6 hours, with nitrogen protection gas flowing in at 20ml / min.

[0083] - Cooling down the resultant solid mixture (also referred to as 3-n-TiO2), and washing preferably three times with 6000 ml deionized water

[0084] - Drying solid 3-n-TiO2, preferably in a vacuum oven at 150°C for 3 hours at reduced pressure, cool down to room temperature and store in an air-tight container.

[0085] Optional further processing steps:

[0086] - Grinding SJE (50g), 3-n-TiO2 (1200g) for 20 minutes at 1000 rpm with intermittent stops in order not to raise temperature too high.

[0087] - Repeat the above step to make enough reaction solid mixture for future use.

[0088] - Mixing 3 kg of this solid mixture and 7 kg of polypropylene (melt index MI = 12g / 10 min)

[0089] - charging the resultant mixture into an extruder (e.g. a twin-screw extruder with 19mm barrel diameter and a Length / Diameter ratio of 44). P5392-PCT October 17, 2024

[0090] - 14 -

[0091] - Extruding and using a pelletizer to cut the extruded strand into approximately 3mm length by 2mm diameter cylindrical pellets (granules) as antimicrobial plastic masterbatch.

[0092] Alternative further processing steps: - Grinding SJE (100g), 3-n-TiO2 (900g) for 20 minutes at 1000 rpm with intermittent stops in order not to raise temperature too high.

[0093] - Repeat the above step to make enough reaction solid mixture for future use.

[0094] - Mixing 3.5 kg of this solid mixture and 6.5 kg of high density polyethylene (melt index MI = 2 g / lOmin) - charging the resultant mixture into an extruder (e.g. a twin-screw extruder with 19mm barrel diameter and a Length / Diameter ratio of 44).

[0095] - Extruding and using a pelletizer to cut the extruded strand into approximately 3mm length by 2mm diameter cylindrical pellets (granules) as antimicrobial plastic masterbatch.

Claims

P5392-PCT October 17, 2024- 15 -WHAT IS CLAIMED IS:

1. Antimicrobial composition comprising a mixture or a reaction product of at least turmeric extract (TME), japonica extract (SJE) and Azithromycin (AZM), wherein the ratio of the mass of turmeric extract (TME) to the mass of japonica extract (SJE), IHTME: msjE is in the range from 1 : 10 to 10: 1, and wherein the ratio of the sum of the masses of turmeric extract (TME) and japonica extract (SJE) to the mass of Azithromycin (AZM), (ITITMI + s.ii ) : mAZM is in the range from 1 : 10 to 10: 1.

2. Antimicrobial composition according to claim 1, characterized in that it comprises nano-particle titanium dioxide n-TiCE or a reaction product of nano-particle titanium dioxide n-TiCE and other components of the composition, and that the ratio of the masses of n-TiCE to the sum of the masses of turmeric extract (TME) and japonica extract (SJE), m-no?: (IUTME + msjE) is in the range from 1 :4 to 4: 1.

3. Antimicrobial composition according to claim 1 or claim 2, characterized in that it comprises N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride or a reaction product of N-trimethoxysilylpropyl-N,N,N- trimethylammonium chloride and other components of the composition.

4. Antimicrobial composition according to any of claims 1 to 3, characterized in that it comprises polyacrylic acid (PAA) and / or low molecular weight polyethylene glycol (PEO) or a reaction product of any of these substances and other components of the composition.

5. Antimicrobial composition according to any of claims 1 to 4, characterized in that it comprises guanidine hydrochloride (GHC1) and / or hexamethyleneP5392-PCT October 17, 2024- 16 - diamine (HMD A) or a reaction product of any of these substances and other components of the composition.

6. Antimicrobial composition according to any of claims 1 to 5, characterized in that it comprises a carrier material selected from polyolefins, including particularly polypropylenes and polyethylenes, copolymers of polyolefins, compounds of polyolefins or copolymers of polyolefins or resins from these substances.

7. Masterbatch in the form of granules or pellets or in the form of a resin comprising an antimicrobial composition according to any one of claims 1 to 6.

8. Antimicrobial product or semi-finished product, characterized in that at least a part of its surface or of its volume comprises an antimicrobial composition according to any of claims 1 to 6.

9. Antimicrobial product or semi-finished product according to claim 8, characterized in that it comprises one of the following: swimming pools or parts thereof, in particular its walls, parts of washing machines, tumblers, refrigerators, dishwashers, water containers, water and ventilation ducts in appliances and buildings, filter devices for purification of water and / or air, sanitary building installation, water treatment systems for drinking water and / or waste water, surfaces in kitchens and / or food processing plants, surfaces in hospitals; parts of water pumps, valves, fans, water and ventilation ducts, pipes, hoses, sealing elements, parts of washing machines, tumble dryers, dishwashers, refrigerators, coffee machines, containers, pails, jars, medical apparatus, cable jacketing; fabrics, towels, facial masks, items of clothing such as socks or underwear, gloves, sportswear, shoes or partsP5392-PCT October 17, 2024- 17 - thereof.

10. Method for manufacturing an antimicrobial composition according to the features of claims 1 to 6, comprising the step of mixing of at least turmeric extract (TME), japonica extract (SJE) and Azithromycin (AZM), wherein the ratio of the mass of turmeric extract (TME) to the mass of japonica extract (SJE), ITITME: msjE is in the range from 1 : 10 to 10: 1, and wherein the ratio of the sum of the masses of turmeric extract (TME) and japonica extract (SJE) to the mass of Azithromycin (AZM), (IHTME + msjE) : ITIAZM is in the range from l:10 to l0:l.

11. Method according to claim 10, characterized in grinding and mixing turmeric extract (TME), japonica extract (SJE) and Azithromycin (AZM) together with nano-particle titanium dioxide (n-TiCE) wherein the ratio of the masses of n- TiCE to the sum of the masses of turmeric extract (TME) and japonica extract(SJE), mTiO2: (HITME + msjE) is in the range from 1 :4 to 4: 1.