Roselle oil composition, and preparation method therefor and use thereof

Abstract

A roselle oil composition, and a preparation method therefor and the use thereof in skin care products. The roselle oil composition comprises, in parts by weight: 90-100 parts of sunflower seed oil and 1-5 parts of roselle extract, wherein the roselle extract has been subjected to a microencapsulation treatment. A preparation method for the roselle oil composition comprises the following steps: slightly heating the sunflower seed oil to 35-40°C, adding the roselle extract thereto, and continuously stirring same until uniform to obtain the composition.

Description

A Roselle Oil Composition, Its Preparation Method and Application

[0001] This application claims priority to Chinese Patent Application No. 202411555743.5, filed on November 4, 2024, entitled "A Roselle Oil Composition and Its Preparation Method and Application", the contents of which are incorporated herein by reference. Technical Field

[0002] This application relates to the field of cosmetics, and more specifically, to a hibiscus oil composition, its preparation method, and its application. Background Technology

[0003] Roselle, scientifically known as Hibiscus rosa-sinensis, is an annual herbaceous plant belonging to the genus Hibiscus in the Malvaceae family. Its unique nutritional value and bioactive components have attracted widespread attention and research in recent years. Roselle has not only been widely cultivated in Guangxi and Yunnan provinces, but its economic and social benefits are also increasingly prominent, particularly demonstrating enormous potential in the food and pharmaceutical development sectors.

[0004] Hibiscus is rich in high-quality protein, various vitamins (such as vitamin C and vitamin A), minerals (potassium, calcium, magnesium, etc.), and fiber. These essential nutrients are crucial for maintaining human health. More notably, hibiscus also contains abundant polyphenols, polysaccharides, organic acids, and other bioactive substances. These components not only endow hibiscus with powerful antioxidant, antibacterial, and anti-inflammatory properties but also lay a solid foundation for its wide application in the medical and cosmetic fields.

[0005] In the field of healthcare, hibiscus has been proven to possess various health benefits due to its diverse bioactive components, including regulating blood pressure, lowering cholesterol, and promoting digestion. Meanwhile, hibiscus extract is widely used in the food industry, such as in the production of hibiscus beverages and pastries, enriching food varieties and enhancing their nutritional value and health benefits. Furthermore, the natural pigments in hibiscus, due to their good stability and high safety, are used for food freshness monitoring, providing a new means of ensuring food safety.

[0006] As consumers increasingly demand safety and efficacy in skincare products, the application of natural plant extracts in skincare is becoming more widespread. Hibiscus, a high-quality plant with both medicinal and edible uses, offers new insights for skincare product development due to its rich nutrients and bioactive substances. Currently, there are no research reports on its application in skincare products. Summary of the Invention

[0007] In order to apply the efficacy of hibiscus to skin care products and meet the current consumer requirements for the safety and efficacy of skin care products, this application provides a hibiscus oil composition, its preparation method and application.

[0008] The hibiscus oil composition provided in this application adopts the following technical solution:

[0009] A roselle oil composition, comprising, by weight, the following components: 90-100 parts sunflower seed oil and 1-5 parts roselle extract;

[0010] The roselle extract is microencapsulated.

[0011] By employing the above-mentioned technical solutions, sunflower seed oil is rich in essential fatty acids, vitamin E, sterols, and other components, providing excellent moisturizing effects. Linoleic acid, as a component of the skin barrier, helps repair and maintain skin barrier function, reducing transepidermal water loss. Vitamin E can lock in moisture, forming a protective film to help the skin stay hydrated for a long time. Hibiscus is rich in polyphenols, flavonoids, and other plant active ingredients, possessing soothing and anti-inflammatory effects, effectively reducing skin redness, sensitivity, and discomfort, thus achieving a soothing effect. Hibiscus extract, such as proanthocyanidins and ursolic acid, has the ability to inhibit inflammatory factors, reducing skin inflammation. These components achieve their anti-inflammatory effect by reducing the release of pro-inflammatory factors (such as IL-1 and TNF-α). Hibiscus extract, such as anthocyanins and quercetin, has strong antioxidant capabilities, scavenging free radicals, reducing oxidative stress, preventing skin cell damage, delaying skin aging, and protecting the skin from environmental pollution and ultraviolet radiation. This complex is composed of sunflower seed oil and hibiscus extract, both purely natural ingredients without any added synthetic chemicals, ensuring the product's safety and gentleness. This combination of natural ingredients meets the modern consumer's demand for natural, safe, and healthy cosmetics.

[0012] Optionally, the microencapsulation process is as follows:

[0013] Roselle flowers were rapidly frozen and dried using liquid nitrogen. The dried roselle flowers were then pulverized into fine powder with a particle size ≤100μm.

[0014] Roselle powder was extracted using supercritical carbon dioxide extraction with 10%–15% ethanol as a co-solvent for 60–90 minutes to obtain the extract.

[0015] The extract and the embedding material were dissolved in ethanol to form an initial mixture. The embedding material was selected from sodium alginate or whey protein. Ultrasonic emulsification was performed at a frequency of 20-25 kHz and a power of 140-160 W for 5-10 minutes to form a uniform microemulsion.

[0016] The microemulsion was spray-dried to form microcapsule particles, thus obtaining roselle extract.

[0017] By employing the above-mentioned technical solution, hibiscus flowers are rapidly frozen and dried using liquid nitrogen before extraction, avoiding the damage to heat-sensitive active substances caused by high temperatures during traditional heat drying, thus ensuring that the extract contains a high concentration of natural antioxidants. Utilizing supercritical carbon dioxide for extraction ensures the integrity and activity of antioxidants such as polyphenols, anthocyanins, and quercetin in the extract. Adding 10%–15% ethanol as a co-solvent further improves extraction efficiency and increases the dissolution of polar compounds. The extract and encapsulation material are then combined using ultrasonic emulsification technology to form a uniform microemulsion, followed by spray drying to form microcapsules. This encapsulation technology protects the active ingredients from oxidation or degradation, extending the product's shelf life while improving its stability and permeability in the skin. The encapsulation material also forms a moisturizing film on the skin surface, further enhancing the product's moisturizing effect. The uniform microemulsion formed by ultrasonic emulsification ensures that the hibiscus extract is evenly dispersed in the encapsulation material, thereby improving the release of active ingredients during use.

[0018] Optional, apple stem cell extract may also be included;

[0019] The mass ratio of the apple stem cell extract to the roselle extract is (0.4-0.6):1.

[0020] Optionally, the preparation method of the apple stem cell extract is as follows:

[0021] Cut the apple peel into small pieces and immerse them in a mixture of cellulase and pectinase. The concentration of cellulase is 0.5%–1.0% (w / v) and the concentration of pectinase is 0.2%–0.5% (w / v). The temperature is controlled at 23–28°C. After completion, the isolated cells are transferred to a culture medium.

[0022] The cells were cultured at 22–25°C. After the stem cell population was formed, an enzymatic extraction method was used, employing 0.1%–0.2% (w / v) esterase, 0.2%–0.5% (w / v) xylanase, and 0.1%–0.2% (w / v) protease at 4–10°C for 24–36 hours.

[0023] After enzymatic extraction, an ultrafiltration membrane with a pore size of 10-50 nm was selected to filter the obtained extract. The concentrated apple stem cell extract was then mixed with nanoliposomes by high-speed stirring to form a uniform nanoparticle suspension.

[0024] The nanoparticle suspension was electrostatically spray-dried, and the dried powder was redispersed in liposomes to obtain the apple stem cell extract.

[0025] By employing the aforementioned technical solution, apple stem cells, rich in plant polyphenols, antioxidants, and other active ingredients, effectively neutralize free radicals and reduce signs of skin aging. Simultaneously, their ability to promote cell regeneration helps repair damaged skin, improving elasticity and firmness. Combined with hibiscus extract, the synergistic effect further enhances the anti-aging effect of the skincare product, improving skin tone and texture. The entire extraction process utilizes natural enzymatic technology and low-temperature processing, reducing the damage to active ingredients caused by chemical solvents and high temperatures, ensuring the product's safety and gentleness. The extracted apple stem cell extract is then mixed with nanoliposomes to form a nanoparticle suspension, which not only protects the stem cell components from external environmental influences (such as oxidation, light, and temperature changes) but also improves the product's absorption into the skin. The small particle size of nanoliposomes allows for better penetration into the deeper layers of the skin. Liposome encapsulation technology not only enhances the permeability of active ingredients but also prevents their loss or oxidation, extending the shelf life and effectiveness of skincare products.

[0026] Optional ingredients also include jojoba oil and avocado oil;

[0027] The mass ratio of jojoba oil, avocado oil and roselle extract is (2-3):(1-1.5):1.

[0028] By employing the above-mentioned technical solutions, jojoba oil penetrates rapidly, providing immediate hydration and regulating sebum function. Avocado oil works on deeper layers of skin tissue, providing long-lasting nourishment and repairing dry and damaged skin barriers. Hibiscus extract, through its anti-inflammatory properties, alleviates redness and irritation caused by weakened skin barrier function, promoting barrier repair at its source. This combination not only provides long-lasting hydration but also helps skin recover from environmental stressors (such as pollution and UV rays), reducing skin burden.

[0029] Hibiscus extract can eliminate free radical damage caused by environmental pollution and ultraviolet radiation, while the deep nourishing and anti-inflammatory effects of avocado oil can further alleviate inflammatory responses caused by oxidative stress. The phytosterols in avocado oil and the anti-inflammatory polyphenols (such as ursolic acid) in hibiscus extract work together to reduce skin inflammation by decreasing the release of pro-inflammatory factors (such as TNF-α and IL-1), resulting in healthier skin and reduced irritation and allergic reactions.

[0030] Jojoba oil, with a structure similar to the skin's natural oils, helps regulate sebum secretion, preventing excessive oiliness while maintaining a proper water-oil balance, making it ideal for oily or combination skin. For oily and combination skin, jojoba oil regulates sebum secretion, helping to reduce shine and clogged pores, while providing adequate hydration. Hibiscus extract, while reducing sebum secretion, can control common oily skin problems such as inflammation and acne through its anti-inflammatory mechanism. Avocado oil's long-lasting nourishing effect ensures that the skin doesn't become dry due to excessive oil control, preventing water-oil imbalance.

[0031] Optional, astaxanthin is also included;

[0032] The mass ratio of astaxanthin to roselle extract is (0.1-0.3):1.

[0033] By employing the above-mentioned technical solutions, astaxanthin can effectively neutralize free radicals in the skin, prevent skin cell damage caused by oxidative stress, and protect skin cells from external factors such as ultraviolet rays and pollution, thereby delaying skin aging. The polyphenols and flavonoids (such as anthocyanins and quercetin) in hibiscus extract already possess strong antioxidant capabilities. When combined with astaxanthin, the two can synergistically enhance the antioxidant effect, effectively reducing the formation of wrinkles and fine lines, while improving skin elasticity and firmness. Astaxanthin not only has antioxidant effects but also possesses certain anti-inflammatory properties. Its synergistic effect with hibiscus extract and jojoba oil significantly enhances the anti-inflammatory and soothing efficacy of the composition.

[0034] Secondly, this application provides a method for preparing a hibiscus oil composition, using the following technical solution:

[0035] A method for preparing a hibiscus oil composition includes the following steps:

[0036] Sunflower seed oil was gently heated between 35 and 40°C. Roselle extract was then added to the sunflower seed oil and stirred continuously until homogeneous to obtain a roselle oil composition.

[0037] Optional, the following steps may be included:

[0038] Sunflower seed oil was gently heated between 35 and 40°C. Roselle extract was added to the sunflower seed oil while stirring. Jojoba oil and avocado oil were added to the mixture in sequence and stirred at low speed for 5 to 10 minutes. Astaxanthin was then added to the sunflower seed oil while maintaining the temperature at 25 to 30°C and stirring at low speed for 5 to 10 minutes. Apple stem cell extract was then added and stirred at low speed for 5 to 10 minutes at 20 to 25°C to obtain the roselle oil composition.

[0039] By adopting the above technical solution, the roselle oil composition has significant antioxidant, anti-aging, repairing and moisturizing effects through the synergistic effect of a variety of natural active ingredients. Furthermore, through scientific temperature control and stirring processes, the activity of the ingredients and the stability of the product are guaranteed, making it suitable for use in high-efficiency skin care products.

[0040] Thirdly, this application provides an application of a hibiscus oil composition, employing the following technical solution:

[0041] Application of a hibiscus oil composition, wherein the hibiscus oil composition is used in a skin care product.

[0042] In summary, this application has the following beneficial effects:

[0043] 1. Because this compound includes hibiscus extract and sunflower seed oil, the combination of natural ingredients enhances the safety and efficacy of the product, while broadening its application range and meeting consumer demand for natural and safe cosmetics. It also synergizes with the soothing and antioxidant effects of hibiscus extract to achieve long-lasting moisturizing and soothing effects on the skin, inhibiting inflammatory factors and suppressing oxidative stress. This compound is purely natural, safe, has good soothing effects, and possesses certain moisturizing and antioxidant properties.

[0044] 2. This application preferably employs a formulation of jojoba oil, avocado oil, and hibiscus extract in a ratio of (2-3):(1-1.5):1. The three ingredients work synergistically to provide comprehensive skincare benefits. Jojoba oil penetrates the skin quickly, providing immediate hydration and regulating sebum production; avocado oil deeply nourishes and repairs dry and damaged skin barriers; hibiscus extract alleviates inflammation and environmental stress-induced damage through its anti-inflammatory and antioxidant mechanisms, helping the skin recover its health. This formula is particularly suitable for regulating sebum secretion, balancing oil and water levels, and ensuring long-lasting nourishment and repair for oily and combination skin.

[0045] 3. This application utilizes innovative extraction processes to ensure the integrity and high efficacy of the active ingredients in hibiscus extract and apple stem cell extract. Hibiscus extract, through liquid nitrogen flash freezing, supercritical carbon dioxide extraction, and ultrasonic emulsification encapsulation technology, effectively retains its antioxidant and soothing properties while enhancing its stability and penetration in the skin. Apple stem cell extract, employing natural enzymatic and low-temperature processing technologies combined with nanoliposome encapsulation, not only retains abundant anti-aging components but also enhances its absorption and effectiveness in deeper layers of the skin. Detailed Implementation

[0046] The following detailed description of this application is provided in conjunction with the embodiments. It should be noted that: unless otherwise specified, the conditions in the following embodiments are performed under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the raw materials used in the following embodiments are all from commercially available sources.

[0047] Example of preparation of hibiscus extract

[0048] Preparation Example 1

[0049] Preparation of a hibiscus extract:

[0050] Fresh roselle flowers are rapidly frozen in liquid nitrogen, and then freeze-dried to remove moisture, resulting in dried roselle flowers.

[0051] The dried roselle flowers were pulverized using a pulverizer until the particle size reached ≤100μm.

[0052] 10 kg of roselle powder was placed in a supercritical carbon dioxide extraction device and extracted at a low temperature of 35°C with a pressure controlled at 22 MPa and a CO2 flow rate maintained at 12 ± 1 L / min. 12% ethanol was added as a co-solvent and the extraction time was 75 minutes to obtain the extract.

[0053] The extract and sodium alginate were dissolved in ethanol to form an initial mixture. The amount of ethanol used was three times the mass of the extract, and the concentration of sodium alginate in the mixture was 3% (w / v). The initial mixture was then treated with ultrasonic emulsification technology at a frequency of 23 kHz, a power of 150 W, and a treatment time of 8 minutes to form a stable microemulsion.

[0054] The microemulsion obtained above was spray-dried using a centrifugal nozzle with atomization pressure controlled at 18 MPa, an airflow rate of 40 L / min, an inlet air temperature of 110–120 °C, and an outlet air temperature of 60–70 °C. After drying, the temperature was lowered to room temperature, and the resulting microcapsule particles were collected by a cyclone separator to obtain roselle extract.

[0055] Preparation Example 2

[0056] Preparation of a roselle extract: The difference from Preparation Example 1 is that whey protein is used as the embedding material, and the concentration of whey protein in the mixture is 8% (w / v).

[0057] Preparation Example 3

[0058] Preparation of a roselle extract: The difference from Preparation Example 1 is that ethanol is not added as a co-solvent.

[0059] Preparation Example 4

[0060] Preparation of a hibiscus extract:

[0061] Preparation of a hibiscus extract:

[0062] Fresh roselle flowers are rapidly frozen in liquid nitrogen, and then freeze-dried to remove moisture, resulting in dried roselle flowers.

[0063] The dried roselle flowers were pulverized using a pulverizer until the particle size reached ≤100μm.

[0064] 10 kg of roselle powder was placed in a supercritical carbon dioxide extraction device and extracted at a low temperature of 35°C with a pressure controlled at 22 MPa and a CO2 flow rate maintained at 12 ± 1 L / min. 12% ethanol was added as a co-solvent and the extraction time was 75 minutes to obtain the extract.

[0065] The extract was filtered through a 30 kDa ultrafiltration membrane to obtain the roselle extract.

[0066] Example of preparation of apple stem cell extract

[0067] Preparation Example 5

[0068] Preparation of an apple stem cell extract:

[0069] Select fresh, undamaged late-ripening apples from Utrecht. Wash them thoroughly and use aseptic techniques to remove the flesh, leaving only the peel. Cut the apple peel into small pieces, approximately 1cm in size. 3 .

[0070] Choose a mixture containing 0.8±0.1% (w / v) cellulase, 0.35±0.1% (w / v) pectinase and 10 mM citrate-sodium citrate buffer, with the remainder being deionized water.

[0071] The temperature of the mixture was controlled at 25℃. Small pieces of apple peel were soaked in the enzyme solution and left to stand for 3 hours. After that, the mixture was centrifuged (5000 rpm for 10 minutes) to remove cell debris and residue, and the apple peel cells were separated. The mixture was then rinsed several times with sterile water to remove residual enzymes and impurities.

[0072] MS medium was used, with Yisheng Biotechnology 61005ES plant gel (plant cell culture grade) selected in the medium. The medium was sterilized in an autoclave at 121℃ for 15 minutes. The isolated apple cells were transferred to the medium and statically cultured at 22-25℃. The suspension culture method was used, with the cells placed on a shaker and gently stirred to promote cell proliferation and aggregation. After 7 days of culture, the apple cells gradually formed cell clusters, which in turn generated a stem cell population.

[0073] The selected enzymatic solution contained the following components: esterase 0.15±0.05% (w / v), xylanase 0.35±0.05% (w / v), protease 0.15±0.05% (w / v), and deionized water as the remaining component. The stem cell population was placed in the enzymatic solution and the enzymatic reaction was carried out at 6±1℃ for 30 hours.

[0074] After enzymatic extraction, cell debris and other insoluble substances were separated by centrifugation (8000 rpm, 15 minutes) to obtain a clear extract containing the effective components of apple stem cells. The enzymatic solution containing stem cells was then filtered using a 30 nm pore size ultrafiltration membrane at an operating pressure of 0.2 MPa.

[0075] The ultrafiltration product was mixed with a 10% (w / v) nano lecithin solution at a mass ratio of 1:3 and stirred at a high speed of 3000 r / min to form a uniform nanoparticle suspension.

[0076] The nanoparticle suspension was processed by an electrostatic spray drying device with the spray drying temperature controlled at 40°C. The dried nanoparticle powder was then redispersed in a lecithin solution with a mass ratio of nanoparticle powder to lecithin solution of 1:2, forming a uniform nanoliposome suspension to obtain apple stem cell extract.

[0077] Example

[0078] Example 1

[0079] A method for preparing a hibiscus oil composition:

[0080] Pour 9 kg of sunflower seed oil (model: Yuanye S24927) into a container and heat slowly to 38℃. During heating, stir slowly at a speed of 50 r / min to ensure the oil is heated evenly.

[0081] After the sunflower seed oil was heated to the target temperature, 0.1 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0082] Continue stirring slowly to ensure that the roselle extract is evenly dispersed in the oil phase. The stirring time should be controlled at 10 minutes to obtain the roselle oil composition.

[0083] Example 2

[0084] A method for preparing a hibiscus oil composition:

[0085] Pour 10 kg of sunflower seed oil (model: Yuanye S24927) into a container and heat it slowly to 38℃. During the heating process, stir slowly at a speed of 50 r / min to ensure that the oil is heated evenly.

[0086] After the sunflower seed oil was heated to the target temperature, 0.5 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0087] Continue stirring slowly to ensure that the roselle extract is evenly dispersed in the oil phase. The stirring time should be controlled at 10 minutes to obtain the roselle oil composition.

[0088] Example 3

[0089] A method for preparing a hibiscus oil composition:

[0090] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38℃. During the heating process, stir slowly at a speed of 50 r / min to ensure that the oil is heated evenly.

[0091] After the sunflower seed oil was heated to the target temperature, 0.3 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0092] Continue stirring slowly to ensure that the roselle extract is evenly dispersed in the oil phase. The stirring time should be controlled at 10 minutes to obtain the roselle oil composition.

[0093] Example 4

[0094] A method for preparing a hibiscus oil composition:

[0095] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0096] After the sunflower seed oil was heated to the target temperature, 0.3 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0097] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract was added. The apple stem cell extract was prepared in Preparation Example 5. The mixture was stirred at a low speed of 50 r / min for 5-10 minutes to obtain the hibiscus oil composition.

[0098] Example 5

[0099] A method for preparing a roselle oil composition: the difference from Example 4 is the addition of 0.12 kg of apple stem cell extract.

[0100] Example 6

[0101] A method for preparing a roselle oil composition: the difference from Example 4 is the addition of 0.18 kg of apple stem cell extract.

[0102] Example 7

[0103] A method for preparing a hibiscus oil composition:

[0104] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0105] After the sunflower seed oil was heated to the target temperature, 0.3 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0106] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract, 0.75 kg of jojoba oil (purchased from Maclean's reagent J885960) and 0.4 kg of avocado oil (purchased from Maclean's reagent A790409) were added. The apple stem cell extract was prepared from Preparation Example 5. The mixture was stirred at a low speed of 50 r / min for 10 minutes to obtain the roselle oil composition.

[0107] Example 8

[0108] A method for preparing a roselle oil composition: the difference from Example 7 is the use of 0.6 kg of jojoba oil and 0.3 kg of avocado oil.

[0109] Example 9

[0110] A method for preparing a roselle oil composition: the difference from Example 7 is the use of 0.9 kg of jojoba oil and 0.45 kg of avocado oil.

[0111] Example 10

[0112] A method for preparing a hibiscus oil composition:

[0113] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0114] After the sunflower seed oil was heated to the target temperature, 0.3 kg of roselle extract, which was prepared in Preparation Example 1, was added.

[0115] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract, 0.75 kg of jojoba oil (purchased from Maclean's reagent J885960), 0.4 kg of avocado oil (purchased from Maclean's reagent A790409), and 0.06 kg of astaxanthin (purchased from Maclean's reagent A832359) were added. The apple stem cell extract was prepared from Preparation Example 5. The mixture was stirred at a low speed of 50 r / min for 10 minutes to obtain the roselle oil composition.

[0116] Example 11

[0117] A method for preparing a roselle oil composition: the difference from Example 10 is the addition of 0.03 kg of astaxanthin.

[0118] Example 12

[0119] A method for preparing a roselle oil composition: the difference from Example 10 is the addition of 0.09 kg of astaxanthin.

[0120] Example 13

[0121] A method for preparing a hibiscus oil composition: The difference from Example 10 is that the hibiscus extract was prepared from Preparation Example 2.

[0122] Comparative Example

[0123] Comparative Example 1

[0124] A method for preparing a hibiscus oil composition: The difference from Example 10 is that the hibiscus extract was prepared by Preparation Example 3.

[0125] Comparative Example 2

[0126] A method for preparing a hibiscus oil composition: The difference from Example 10 is that the hibiscus extract was prepared by Preparation Example 4.

[0127] Comparative Example 3

[0128] A method for preparing a hibiscus oil composition: The difference from Example 10 is that the apple stem cell extract used is PhytoCellTec from the Swiss Miberl Group. TM Malus Domestica apple stem cells.

[0129] Performance testing

[0130] Detection methods

[0131] (1) Moisturizing effect test:

[0132] Thirty participants of different genders, ages, and skin types were selected, excluding those with skin diseases, allergies, or other conditions unsuitable for the test. The inner forearm was used for skin testing. Each participant applied hibiscus oil complexes from Examples 1-13 and Comparative Examples 1-3 to the same designated area, with identical application area and thickness, and adjacent application areas spaced 1 cm apart. A professional Corneometer CM 825 was used to measure skin moisture content, assessing skin hydration status by measuring the moisture content of the stratum corneum; data is typically displayed in units (AU).

[0133] Before the first use of the product, the baseline moisture level of each test subject's test area was measured, and the skin moisture content before product use was recorded. Moisture content was measured at 4h, 8h, and 12h after application, and the moisture content of the skin in the test area was recorded to calculate its water retention rate.

[0134] Table 1 Moisturizing effect test

[0135] (2) DPPH free radical scavenging rate test

[0136] Preparation of DPPH ethanol solution: Weigh 20 mg DPPH, dissolve in anhydrous ethanol, and dilute to a final volume of 250 mL in a volumetric flask. The DPPH concentration is prepared to be 2 × 10⁻⁶. -4 mol / L; store protected from light at 0–4℃, prepare and use immediately, effective within 4 hours; (positive control: use vitamin C at the same concentration)

[0137] Preparation of the test solution: Prepare four concentrations, diluted 10, 20, 50, and 100 times respectively. Take 1.0 mL of the test solution and mix it with 1.0 mL of 2×10⁻⁶ solution. -4 Mix 1.0 mL of anhydrous ethanol with 1.0 mL of 2 × 10⁻⁶ mol / L DPPH solution (tube A); -4 Mix 1 mol / L DPPH solution (tube B); mix 1.0 mL of anhydrous ethanol with 1.0 mL of the test solution (tube C); after reacting for 30 min, measure the absorbance values ​​of tubes A, B, and C at 517 nm and calculate the clearance rate.

[0138] The calculation formula is: Clearance rate (%) = [(B+C)-A] / B

[0139] (3) HaCaT cell viability test

[0140] HaCaT cells were seeded into 96-well plates, with 5,000 to 10,000 cells per well, and cultured for 24 hours to ensure cell adhesion.

[0141] The roselle oil complexes prepared in Examples 1-13 and Comparative Examples 1-3 were used to treat HaCaT cells for 24 hours.

[0142] After treatment, CCK-8 reagent was added to the cells and incubated for 2 hours. The optical density (OD value) at 450 nm was measured using a microplate reader, and this value is directly proportional to cell viability. If the hibiscus oil complex did not significantly inhibit cell viability, it indicates that it has good safety for HaCaT cells.

[0143] (4) Inflammatory factor test

[0144] HaCaT cells were cultured in 6-well plates, 1 × 10⁶ cells per well. 5 Cells. Cells were treated with a hibiscus oil complex for 24 hours.

[0145] After treatment, LPS (1 μg / mL) was added to stimulate the cells, and they were incubated for 24 hours to induce an inflammatory response.

[0146] Total RNA was extracted from cells and reverse transcribed to generate cDNA. The gene expression of the inflammatory cytokine IL-6 was detected using qPCR, and the relative gene expression level was analyzed using the ΔCt method.

[0147] Collect cell culture supernatant and use an ELISA kit to detect the IL-6 content in the supernatant.

[0148] The calculation method is as follows:

[0149] Relative expression level of inflammatory factors (%) = Inflammatory factor level in the treatment group / Inflammatory factor level in the LPS group * 100%

[0150] If the hibiscus oil complex significantly reduces the level of LPS-induced inflammatory factors, it indicates that it has an anti-inflammatory effect.

[0151] Table 2 Clearance rate / cell viability / inflammatory factor test data

[0152] As can be seen from Examples 3 and Comparative Examples 1-2, and Tables 1-2, adding 10%-15% ethanol as a co-solvent further improves extraction efficiency and increases the dissolution rate of polar compounds. The extract and encapsulation material are combined using ultrasonic emulsification to form a uniform microemulsion, which is then spray-dried to form microcapsule particles. This encapsulation technique protects the active ingredients from oxidation or degradation, extending the product's shelf life and improving its stability and permeability in the skin. The encapsulation material also forms a moisturizing film on the skin surface, further enhancing the product's moisturizing effect. The uniform microemulsion formed by ultrasonic emulsification ensures that the hibiscus extract is evenly dispersed in the encapsulation material, thereby improving the release effect of the active ingredients during use.

[0153] As can be seen from Example 4 and Comparative Example 3, and in conjunction with Tables 1-2, the entire extraction process utilizes natural enzymatic technology and low-temperature treatment, reducing the damage to active ingredients caused by chemical solvents and high temperatures, thus ensuring the safety and gentleness of the product. The extracted apple stem cell extract, when mixed with nanoliposomes, forms a nanoparticle suspension, which not only protects the stem cell components from external environmental influences but also enhances the product's absorption into the skin. The small particle size of the nanoliposomes allows for better penetration into the deeper layers of the skin. Liposome encapsulation technology not only improves the permeability of active ingredients but also prevents their loss or oxidation, extending the shelf life and effectiveness of skincare products.

[0154] As can be seen from Examples 1-3 and Tables 1-2, the combination of sunflower seed oil and roselle extract effectively achieves soothing and antioxidant effects, and has excellent long-lasting moisturizing effects. All the ingredients used are pure natural ingredients, without the addition of synthetic chemicals, thus ensuring the safety and gentleness of the product.

[0155] As can be seen from Examples 3-6 and Tables 1-2, apple stem cells are rich in plant polyphenols, antioxidants, and other active ingredients, which can effectively neutralize free radicals and reduce signs of skin aging. Simultaneously, their ability to promote cell regeneration helps repair damaged skin and improve skin elasticity and firmness. When used in combination with hibiscus extract, the synergistic effect further enhances the anti-aging effect of the skincare product, improving skin tone and texture.

[0156] As can be seen from Examples 4 and 7-9, and Tables 1-2, the formulation using a ratio of (2-3):(1-1.5):1 of jojoba oil, avocado oil, and hibiscus extract not only provides immediate and deep hydration but also exhibits excellent anti-inflammatory and antioxidant effects. Their synergistic effect helps the skin maintain its oil-water balance, reduce inflammation, and promote skin barrier repair while resisting pollution, UV rays, and oxidative stress. This combination is ideal for oily, combination, and sensitive skin, comprehensively improving skin health.

[0157] As can be seen from Examples 7 and 10-12, and in conjunction with Tables 1-2, the polyphenols and flavonoids (such as anthocyanins and quercetin) in hibiscus extract already possess strong antioxidant capabilities. When combined with astaxanthin, the two can synergistically enhance the antioxidant effect, effectively reducing the formation of wrinkles and fine lines, while improving skin elasticity and firmness. Astaxanthin not only has antioxidant effects but also possesses certain anti-inflammatory properties. Its synergistic effect with hibiscus extract and jojoba oil significantly enhances the anti-inflammatory and soothing efficacy of the composition.

[0158] As can be seen from Examples 10 and 13 and Tables 1-2, sodium alginate was selected as the embedding material in this application, which achieved better results and enabled the composition to have better moisturizing and antioxidant effects.

[0159] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A roselle oil composition, characterized in that, By weight, it includes the following components: 90-100 parts sunflower seed oil and 1-5 parts roselle extract; The roselle extract is microencapsulated.

2. The hibiscus oil composition according to claim 1, characterized in that, The microencapsulation process is as follows: Roselle flowers were rapidly frozen and dried using liquid nitrogen. The dried roselle flowers were then pulverized to a particle size of ≤100μm to obtain roselle powder. The roselle powder was extracted using supercritical carbon dioxide extraction, and 10%–15% ethanol was added as a co-solvent to obtain the extract. The extract and the embedding material are dissolved in ethanol to form an initial mixture; the embedding material is selected from sodium alginate or whey protein. The initial mixture is ultrasonically emulsified to form a uniform microemulsion; The microemulsion was spray-dried to form microcapsule particles, thus obtaining roselle extract.

3. The hibiscus oil composition according to claim 2, characterized in that, The supercritical carbon dioxide extraction time is 60–90 min.

4. The hibiscus oil composition according to claim 2, characterized in that, The ultrasonic emulsification frequency is 20–25 kHz, the power is 140–160 W, and the emulsification time is 5–10 min.

5. The hibiscus oil composition according to claim 1, characterized in that, It also includes apple stem cell extract; The mass ratio of the apple stem cell extract to the roselle extract is (0.4-0.6):

1.

6. The hibiscus oil composition according to claim 5, characterized in that: The preparation method of the apple stem cell extract is as follows: Apple peels were cut into small pieces and immersed in a mixture of cellulase and pectinase. After enzymatic hydrolysis, the separated cells were transferred to a culture medium and cultured until a stem cell population was formed. The formed stem cell population was then enzymatically extracted and filtered through an ultrafiltration membrane to obtain a concentrated apple stem cell extract. The concentrated apple stem cell extract was mixed with nanoliposomes by high-speed stirring to form a uniform nanoparticle suspension. The nanoparticle suspension was electrostatically spray-dried, and the dried powder was redispersed in liposomes to obtain the apple stem cell extract.

7. The roselle composition according to claim 6, characterized in that, The concentration of cellulase in the mixture of cellulase and pectinase is 0.5% to 1.0% (w / v), the concentration of pectinase is 0.2% to 0.5% (w / v), and the temperature is 23 to 28°C.

8. The roselle composition according to claim 6, characterized in that, The culture temperature is 22–25°C.

9. The roselle composition according to claim 6, characterized in that, The enzymes used in the enzymatic extraction are 0.1%–0.2% (w / v) esterase, 0.2%–0.5% (w / v) xylanase, and 0.1%–0.2% (w / v) protease.

10. The roselle composition according to claim 6 or 9, characterized in that, The enzymatic extraction temperature is 4–10℃, and the processing time is 24–36 h.

11. The roselle composition according to claim 6, characterized in that, The ultrafiltration membrane has a pore size of 10–50 nm.

12. The roselle oil composition according to claim 1, characterized in that: It also includes jojoba oil and avocado oil; The mass ratio of jojoba oil, avocado oil and roselle extract is (2-3):(1-1.5):

1.

13. The hibiscus oil composition according to claim 1, characterized in that: It also includes astaxanthin; The mass ratio of astaxanthin to roselle extract is (0.1-0.3):

1.

14. A method for preparing the hibiscus oil composition according to any one of claims 1 to 4, characterized in that: Includes the following steps: After gently heating sunflower seed oil between 35-40°C, hibiscus extract is added and stirred continuously until homogeneous to obtain the hibiscus oil composition.

15. The preparation method according to claim 14, characterized in that: The preparation method of the roselle oil composition includes the following steps: Sunflower seed oil was gently heated between 35-40℃, hibiscus extract was added, and stirring was maintained. Jojoba oil and avocado oil were added in sequence, and stirring was carried out at low speed for 5-10 minutes. Astaxanthin was then added, and the temperature was maintained at 25-30℃, and stirring was carried out at low speed for 5-10 minutes. Apple stem cell extract was then added, and stirring was carried out at low speed for 5-10 minutes at 20-25℃ to obtain the hibiscus oil composition.

16. The use of the hibiscus oil composition according to any one of claims 1-13 in a skin care product.

Images