Solubilzation composition of titrated extract of centella asiatica

Abstract

According to an embodiment of the present application, provided is a solubilization composition of a titrated extract of Centella Asiatica. The solubilization composition of a titrated extract of Centella Asiatica comprises a titrated extract of Centella Asiatica (TECA), a solubilizing agent, and a solubilization aid, wherein the solubilizing agent comprises a glycolipid, the glycolipid comprises a mannosylerythritol lipid, and the solubilization aid may comprise dipotassium glycyrrhizate. According to embodiments of the present application, a titrated extract of Centella Asiatica, which is a substance with poor solubility, can be uniformly solubilized, and thus can be stabilized in a transparent or translucent form without precipitation.

Description

Solubilized composition of quantitative extract of Centella asiatica

[0001] This application relates to a solubilized composition of quantitative extract of Centella asiatica.

[0002] Titrated extract of Centella Asiatica (TECA) is a substance obtained by quantifying active ingredients from Centella Asiatica at a specific ratio. Titrated extract of Centella Asiatica possesses various benefits, such as skin damage repair, anti-inflammatory, and antioxidant effects, and is utilized as a material for cosmetics and pharmaceuticals to aid in skin improvement and recovery. However, TECA is inherently a poorly soluble substance, and there is a problem in that it is difficult to use because it does not disperse uniformly in water-soluble media and precipitates easily.

[0003] Surfactants are generally used to uniformly disperse poorly soluble substances in water-soluble media. While surfactants aid in solubilization by lowering the water-oil boundary, synthetic surfactants can cause skin irritation and may lead to side effects, such as damage to the skin barrier with long-term use. Furthermore, as some synthetic surfactants can have harmful effects on the environment, the use of eco-friendly and safe ingredients has recently become increasingly important.

[0004] Therefore, there is a need to develop a method to solubilize the quantitative extract of Centella asiatica, a poorly soluble substance, using natural raw materials.

[0005] The purpose of this application is to provide a solubilized composition of quantitative extract of Centella asiatica.

[0006] According to an embodiment of the present application, a titrated extract solubilization composition of Centella Asiatica is provided. The titrated extract solubilization composition of Centella Asiatica comprises a titrated extract of Centella Asiatica (TECA), a solubilizing agent, and a solubilizing aid, wherein the solubilizing agent comprises a glycolipid, the glycolipid comprises a mannosylerythritol lipid, and the solubilizing aid may comprise a dipotassium glycyrrhizate.

[0007] In addition, the weight ratio of the above mannosylerythritol lipid and the above dipotassium glycyrrhizate may be 1:0.5 to 7.5.

[0008] In addition, the above mannosylerythritol lipid may be included in an amount of 0.2 to 10 weight percent based on the total weight of the composition.

[0009] In addition, the above-mentioned dipotassium glycyrrhizate may be included in an amount of 1 to 15 weight percent relative to the total weight of the composition.

[0010] In addition, the above quantitative extract of Centella asiatica may be included in an amount of 0.1 to 5 weight percent relative to the total weight of the composition.

[0011] In addition, the above-mentioned solubilized composition of the quantitative extract of Centella asiatica may have an average particle size of 130 nm or less as measured by the Dynamic Light Scattering (DLS) method.

[0012] In addition, the above-mentioned solubilized composition of the quantitative extract of Centella asiatica may have a polydispersity index of 0.140 to 0.260 as measured by the Dynamic Light Scattering (DLS) method.

[0013] According to an embodiment of the present application, a cosmetic composition comprising the above-mentioned quantitative extract solubilized composition of Centella asiatica is provided.

[0014] According to the embodiments of the present application, as the quantitative extract of Centella asiatica, which is a poorly soluble substance, is uniformly solubilized, it can be stabilized into a transparent or translucent state without precipitation.

[0015] The effects obtainable from the embodiments of the present application are not limited to those mentioned above, and other unmentioned effects will be clearly understood by those skilled in the art to which the present application belongs from the description below.

[0016] FIGS. 1 to 3 are photographs showing a composition for solubilizing quantitative extract of Centella asiatica according to the examples and comparative examples of the present application.

[0017] The structural or functional descriptions of the embodiments disclosed in this specification are merely illustrative for the purpose of explaining embodiments according to the technical concept of this application. Furthermore, embodiments according to the technical concept of this application may be implemented in various forms other than those disclosed in this specification. Additionally, the technical concept of this application is not to be interpreted as being limited to the embodiments described in this specification.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention pertains.

[0019] Among the physical properties mentioned in this specification, if the measurement temperature affects the property, the property is the one measured at room temperature and pressure unless otherwise specifically stipulated.

[0020] Unless otherwise specifically defined among the physical properties mentioned in this specification, said physical properties may basically follow the SI unit system. For example, weight may be expressed in kg units, and length may be expressed in m units.

[0021] The term "room temperature" as used in this specification refers to a natural temperature that is not heated or cooled, and may mean, for example, any temperature within the range of 10°C to 30°C, for example, about 15°C or higher, about 18°C ​​or higher, about 20°C or higher, about 23°C or higher, about 27°C or lower, or 25°C. Unless specifically defined in this specification, the unit of temperature is Celsius (°C).

[0022] The term "relatively high temperature relative to room temperature" as used in this specification refers to a temperature higher than room temperature, and may mean, for example, any temperature within the range of 30°C to 50°C, for example, about 35°C or higher, about 40°C or higher, or about 45°C or lower.

[0023] Among the physical properties mentioned in this specification, if the measured pressure affects the physical property, unless otherwise specifically defined, the physical property is the property measured at atmospheric pressure. The term atmospheric pressure used in this specification refers to natural pressure that is not pressurized or depressurized, and typically refers to atmospheric pressure within the range of about 700 mmHg to 800 mmHg.

[0024] As used in this specification, the terms a to b refer to a range between a and b, including a and b. For example, including a to b parts by weight is equivalent to including within the range of a to b parts by weight.

[0025] Throughout this specification, values ​​expressed in the form of ranges should be interpreted in a flexible manner to include not only numeric values ​​explicitly mentioned as limits of such ranges, but also all individual numeric values ​​or sub-ranges included within such ranges, as if each individual numeric value and sub-range were explicitly mentioned. For example, a range of “about 0.1% to about 5%” or “about 0.1% to 5%” should be interpreted to include not only about 0.1% to about 5%, but also individual values ​​(e.g., 1%, 2%, 3%, and 4%) and sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range. Unless otherwise indicated, a reference to “about X to Y” has the same meaning as “about X to about Y.” Likewise, unless otherwise indicated, a reference to “about X, Y, or about Z” has the same meaning as “about X, about Y, or about Z.”

[0026] The terms “about” or “approximately” as used herein mean that dimensions, sizes, formulations, parameters, shapes, and other quantities and characteristics may not be precise and may be approximate and / or larger or smaller by reflecting, as necessary, tolerances, conversion factors, rounding, measurement errors, and other factors known to those skilled in the art. The terms “about” or “approximately” apply to the dimensions, sizes, formulations, parameters, shapes, or other quantities or characteristics described herein, generally, regardless of whether they are explicitly mentioned. The term “about” as used herein may allow for a degree of variability in a given value or range, for example, within 10%, within 5%, or within 1% of the stated value or stated range limit, and includes the stated precise value or range.

[0027] Where a method for measuring or evaluating specific dimensions, sizes, formulations, parameters, shapes, and other quantities and characteristics is not described in this specification, a person skilled in the art may readily recognize a previously known method for measuring or evaluating, or may readily derive such method by referring to prior art known prior to the filing date of this specification.

[0028] As used herein, the term “substantially” refers to a majority or most, such as at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, 99.99%, or at least about 99.999% or more, or 100%. As used herein, the term “substantially absent” may mean having nothing, or having a negligible amount of a material present such that the amount of the material does not affect the substantial properties of the composition containing the material. At this time, a small amount is an amount of material in the composition of about 0% to about 5% by weight, or about 0% to about 1% by weight, or about 5% or less by weight, or about 4.5% or less by weight, or about 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.01, or 0.001% by weight or less. The term “substantially absent” as used herein may mean that the composition has about 0% by weight to about 5% by weight of material, or about 0% by weight to about 1% by weight, or about 5% by weight or less, or about 4.5% by weight or less, about 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.01, or 0.001% by weight or less, or a minor amount having about 0% by weight.

[0029] As used in this specification, the term “generally” means 50% or more, 60% or more, 70% or more, 80% or more, or 90% or more.

[0030] The terms used herein are for describing embodiments and are not intended to limit or / or restrict the present application. Singular expressions include plural expressions unless the context clearly indicates otherwise. Additionally, numbers used herein (e.g., First, Second, etc.) are merely identifiers to distinguish one component from another.

[0031] In this specification, when a part is described as being connected to another part, this includes not only cases where they are directly connected, but also cases where they are indirectly connected with other components in between. Furthermore, when a part is described as including a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0032] Furthermore, in this application, the term “or” is intended to mean an implied “or” rather than an exclusive “or.” That is, unless otherwise specified or evident from the context, “X uses A or B” is intended to mean one of the natural implied substitutions. In other words, if X uses A; if X uses B; or if X uses both A and B, “X uses A or B” may apply to any of these cases. Additionally, the term “and / or” as used herein should be understood to refer to and include all possible combinations of one or more of the enumerated related configurations.

[0033]

[0034] According to an embodiment of the present application, a solubilized composition of titrated extract of Centella Asiatica is provided. The solubilized composition of titrated extract of Centella Asiatica comprises titrated extract of Centella Asiatica (TECA), which is a poorly soluble substance, wherein the titrated extract of Centella Asiatica is stably solubilized so that no precipitation phenomenon may occur. Titrated extract of Centella Asiatica is a substance obtained by quantitatively extracting active ingredients derived from Centella Asiatica. Titrated extract of Centella Asiatica contains triterpenes such as asiaticoside, madecassoside, asiatic acid, and madecassic acid. Titrated extract of Centella Asiatica has efficacy such as skin regeneration, anti-inflammatory, antioxidant, and collagen synthesis promotion, and is widely used in products for skin damage repair, anti-aging, anti-inflammation, and irritation relief.

[0035] In the examples, the quantitative extract of Centella asiatica may be included in an amount of 0.1 to 5 weight% relative to the total weight of the composition. Solubilization can proceed stably within the above range. For example, the content of the quantitative extract of Centella asiatica may be 0.1 weight% or more, 0.5 weight% or more, or 1 weight% or more. For example, the content of the quantitative extract of Centella asiatica may be 5 weight% or less, 3 weight% or less, 2 weight% or less, or 1 weight% or less.

[0036] In the examples, the solubilization composition of the quantitative extract of Centella asiatica may include a solubilizing agent and a solubilization aid. The solubilizing agent is used to mix poorly soluble substances with a polar solvent (water) to create a homogeneous solution state, and its use allows for obtaining a transparent or translucent formulation. The solubilization aid is used in conjunction with the solubilizing agent and can enhance the solubilization effect or improve stability, and can complement the function of the solubilizing agent to increase the solubility and transparency of specific components. According to the examples of the present application, the quantitative extract of Centella asiatica can be stably solubilized by using the solubilizing agent and the solubilization aid together.

[0037] In the examples, the solubilizing agent may include glycolipids. Glycolipids are biomolecules in which sugar and lipid are combined, and are mainly found as components of cell membranes. Glycolipids can solubilize the quantitative extract of Centella asiatica, which is a poorly soluble substance, in water by, for example, forming micelles or liposomes in water and loading the quantitative extract of Centella asiatica inside. According to the examples of the present application, by using glycolipids, which are natural raw materials, instead of synthetic surfactants such as polyethylene glycol as a solubilizing agent, biocompatibility and eco-friendliness can be satisfied, and additionally, the solubilizing efficacy for the quantitative extract of Centella asiatica can be improved.

[0038] In the examples, the glycolipid may include mannosylerythritol lipid. When mannosylerythritol lipid is used, the solubilization efficacy for the quantitative extract of Centella asiatica can be maximized.

[0039] In the examples, the solubilizing agent may include dipotassium glycyrrhizate. When dipotassium glycyrrhizate is used, the solubilizing enhancement efficacy for the quantitative extract of Centella asiatica can be maximized.

[0040] In the examples, the weight ratio of mannosylerythritol lipid to dipotassium glycyrrhizate may be 1:0.5 to 7.5. In this specification, the weight ratio of A and B means A:B based on weight. For example, the weight ratio of mannosylerythritol lipid to dipotassium glycyrrhizate may be 1:0.5 or more, 1:1 or more, 1:2 or more, 1:3 or more, 1:4 or more, 1:5 or more, 1:6 or more, or 1:7 or more. For example, the weight ratio of mannosylerythritol lipid to dipotassium glycyrrhizate may be 1:0.75 or less, 1:0.7 or less, 1:0.6 or less, 1:0.5 or less, 1:0.4 or less, 1:0.3 or less, 1:0.2 or less, or 1:0.1 or less. Within the above ranges, the solubilization efficacy for the quantitative extract of Centella asiatica can be maximized, and the quantitative extract of Centella asiatica can be solubilized to have a small and uniform particle size.

[0041] In the examples, the weight ratio of the quantitative extract of Centella asiatica and the mannosylerythritol lipid may be 1:1 to 4. For example, the weight ratio of the quantitative extract of Centella asiatica and the mannosylerythritol lipid may be 1:1 or higher, 1:1.5 or higher, or 1:2 or higher. For example, the weight ratio of the quantitative extract of Centella asiatica and the mannosylerythritol lipid may be 1:4 or lower, 1:3.5 or lower, 1:3 or lower, 1:2.5 or lower, or 1:2 or lower. Within the above ranges, the solubilization efficacy for the quantitative extract of Centella asiatica can be maximized, and the quantitative extract of Centella asiatica can be solubilized to have a small and uniform particle size.

[0042] In the examples, mannosylerythritol lipids may be included in an amount of 0.2 to 10 weight% relative to the total weight of the composition. For example, the mannosylerythritol lipid content may be 0.2 weight% or more, 0.5 weight% or more, 1 weight% or more, 2 weight% or more, 3 weight% or more, 4 weight% or more, 5 weight% or more, 6 weight% or more, 7 weight% or more, 8 weight% or more, or 9 weight% or more. For example, the mannosylerythritol lipid content may be 10 weight% or less, 9 weight% or less, 8 weight% or less, 7 weight% or less, 6 weight% or less, 5 weight% or less, 4 weight% or less, 3 weight% or less, 2 weight% or less, 1 weight% or less, or 0.5 weight% or less. Within the above ranges, the solubilization efficacy for the quantitative extract of Centella asiatica can be maximized, and the quantitative extract of Centella asiatica can be solubilized to have a small and uniform particle size.

[0043] In the examples, dipotassium glycyrrhizate may be included in an amount of 1 to 15 weight% relative to the total weight of the composition. For example, the dipotassium glycyrrhizate content may be 1 weight% or more, 2 weight% or more, 3 weight% or more, 4 weight% or more, 5 weight% or more, 6 weight% or more, 7 weight% or more, 8 weight% or more, 9 weight% or more, 10 weight% or more, 11 weight% or more, 12 weight% or more, 13 weight% or more, or 14 weight% or more. For example, the dipotassium glycyrrhizate content may be 15 weight% or less, 14 weight% or less, 13 weight% or less, 12 weight% or less, 11 weight% or less, 10 weight% or less, 9 weight% or less, 8 weight% or less, 7 weight% or less, 6 weight% or less, 5 weight% or less, 4 weight% or less, 3 weight% or less, or 2 weight% or less. Within the above range, the solubilization efficacy for the quantitative extract of Centella asiatica can be maximized, and the quantitative extract of Centella asiatica can be solubilized to have a small and uniform particle size.

[0044] In the examples, the solubilizing composition of the quantitative extract of Centella asiatica may have an average particle size of 130 nm or less as measured by the Dynamic Light Scattering (DLS) method. For example, the average particle size may be the average particle size of micelles or liposomes formed within the solubilizing composition of the quantitative extract of Centella asiatica. For example, the average particle size of the solubilizing composition of the quantitative extract of Centella asiatica may be 130 nm or less, 120 nm or less, 110 nm or less, 100 nm or less, 90 nm or less, 80 nm or less, 70 nm or less, 60 nm or less, 50 nm or less, 40 nm or less, or 30 nm or less. For example, the average particle size of the solubilizing composition of the quantitative extract of Centella asiatica may be 1 nm or more, 5 nm or more, 10 nm or more, or 20 nm or more. For example, the average particle size of the solubilizing composition of the quantitative extract of Centella asiatica may be 1 to 130 nm. If the average particle size falls within the above range, it can be understood that solubilization has proceeded sufficiently, resulting in a small average particle size.

[0045] In addition, the above-mentioned solubilizing composition of the quantitative extract of Centella asiatica may have a polydispersity index of 0.140 to 0.260 as measured by the Dynamic Light Scattering (DLS) method. For example, the polydispersity index may be the polydispersity index of micelles or liposomes formed within the solubilizing composition of the quantitative extract of Centella asiatica. For example, the polydispersity index of the solubilizing composition of the quantitative extract of Centella asiatica may be 0.140 or higher, 0.150 or higher, 0.160 or higher, 0.170 or higher, 0.180 or higher, 0.190 or higher, 0.200 or higher, 0.210 or higher, 0.220 or higher, 0.230 or higher, 0.240 or higher, or 0.250 or higher. For example, the polydispersity index of the solubilized composition of the quantitative extract of Centella asiatica may be 0.260 or less, 0.250 or less, 0.240 or less, 0.230 or less, 0.220 or less, 0.210 or less, 0.200 or less, 0.190 or less, 0.180 or less, 0.170 or less, 0.160 or less, or 0.150 or less. If the polydispersity index falls within the above range, it can be understood that the particles have been solubilized to have a uniform size.

[0046]

[0047] According to an embodiment of the present application, a cosmetic composition comprising a solubilized composition of a quantitative extract of Centella asiatica is provided. The solubilized composition of the quantitative extract of Centella asiatica may be included, for example, in an amount of 0.001% to 100% by weight based on the total weight of the cosmetic composition.

[0048] In the examples, the cosmetic composition may further include additives commonly used in the art within a range that does not impair the effects of the examples of the present application. For example, the cosmetic composition may further include thickeners, pH adjusters, surfactants, stabilizers, preservatives, antiseptics, disinfectants, moisturizers, sunscreens, skin conditioning agents, whitening agents, coloring agents, organic pigments, inorganic pigments, fragrances, vitamins, natural colorants, pearls, and other functional ingredients. Here, the functional ingredients may include, for example, wrinkle-improving ingredients, whitening ingredients, antioxidant ingredients, anti-aging ingredients, plumping ingredients, exfoliating ingredients, scrub ingredients, moisturizing ingredients, skin barrier strengthening ingredients, skin elasticity-enhancing ingredients, etc. The amount of the above ingredients can be easily selected by a person skilled in the art within a range that does not impair the purpose and effects of the present application.

[0049] In the examples, the cosmetic composition may be provided in formulations commonly manufactured in the art. For example, the cosmetic composition may be formulated into an oil-dispersed formulation in which a powder complex is dispersed in an oil phase, a water-dispersed formulation in which a powder complex is dispersed in an aqueous phase, an emulsion formulation of the type of oil in water (O / W), water in oil (W / O), oil in water in water (W / O / W), and oil in water in oil (O / W / O), a solubilized formulation, etc. However, it is not limited thereto, and the cosmetic composition may also be provided in formulations such as a solution, suspension, emulsion, solid, gel, oil, powder, paste, or foam aerosol.

[0050] In the examples, the cosmetic composition can be manufactured into various products. For example, it can be manufactured into sun care products such as sun cream, sun stick, sun milk, sun lotion, and sun cushion. For example, it can be manufactured into functional cosmetics such as nourishing cream, essence, and ampoule, or into basic cosmetics such as toner and lotion. In addition, it can be manufactured into soap, cleanser, cleansing cream, cleansing water, etc., or into color cosmetics such as lipstick, lip balm, mascara, blush, shading, highlighter, makeup base, foundation, compact powder, concealer, skin cover, etc. Furthermore, it can be manufactured into hair cleansing products such as shampoo, rinse, hair conditioner, hair gel, etc. Additionally, it can be manufactured into products that are attached to the skin, such as packs and hydrogel slimming patches, or products that are sprayed onto the skin, such as mist and aerosol.

[0051] In the examples, the composition may be a topical skin composition. For example, the topical skin composition may have a formulation such as a solution, oil, paste, cream, gel, patch, spray, etc.

[0052] In the examples, the skin topical composition may appropriately incorporate ingredients commonly used in skin topical preparations such as cosmetics or pharmaceuticals, for example, aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, sunscreens, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, fatty substances, solvents, thickeners, gelling agents, emollients, foaming agents, fragrances, metal ion chelating agents, chelating agents, vitamins, various skin nutrients, or combinations thereof as needed. The amount of the above ingredients can be easily selected by a person skilled in the art within a range that does not impair the purpose and effect of the present application.

[0053]

[0054] The present invention will be explained below through manufacturing examples and experimental examples, but the scope of the present invention is not limited by the contents presented below.

[0055]

[0056] Preparation Example

[0057]

[0058] (1) Preparation Example 1: Preparation of Examples 1 to 9

[0059] Solubilized compositions of the quantitative extract of Centella asiatica in Examples 1 to 9 were prepared according to the contents listed in Tables 1 and 2. Specifically, the quantitative extract of Centella asiatica and a solubilizing agent were mixed and added to purified water, and then a solubilizing aid was added. At this time, the mixture was heated using a disperser mixer. The units of the contents in Tables 1 and 2 are weight%.

[0060]

[0061] Classification Example 1 Example 2 Example 3 Example 4 Example 5 Solubilizing agent Mannosyl erythritol lipid 2 2 2 2 Solubilizing adjuvant Dipotassium glycyrrhizate 1 0 1 5 4 2 1 Quantitative extract of Centella asiatica 1 1 1 1 1 Purified water To 100 To 100 To 100 To 100 To 100

[0062]

[0063] Classification Example 6 Example 7 Example 8 Example 9 Solubilizing agent Mannosyl erythritol lipid 0.2 1 / 4 10 Solubilizing adjuvant Dipotassium glycyrrhizate 11 4 10 Quantitative extract of Centella asiatica 0.1 0.5 25 Purified water To 100 To 100 To 100 To 100

[0064]

[0065] (2) Preparation Example 2: Preparation of Comparative Examples 1 to 14

[0066] Solubilized compositions of the quantitative extract of *Clematis terniflora* of Comparative Examples 1 to 14 were prepared according to the contents listed in Tables 3 and 4 using the same method as in Preparation Example 1. The units of the contents in Tables 3 and 4 are weight%.

[0067]

[0068] Classification Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Solubilizing Agent Mannosyl Erythritol Lipid-222 0.115 Solubilizing Adjuvant Dipotassium Glycyrrhizate 10-0.1 0.5 2044 Quantitative Extract of Centella Asiatica 1111111 Purified Water To 100 To 100 To 100 To 100 To 100 To 100 To 100

[0069]

[0070] Classification Comparative Example 8 Comparative Example 9 Comparative Example 10 Comparative Example 11 Comparative Example 12 Comparative Example 13 Comparative Example 14 Solubilizing Agent Mannosyl Erythritol Lipid--222--Lecithin 2----22 Hydrogenated Lecithin-2-----Solubilizing Auxiliary Dipotassium Glycyrrhizate 44-----Lecithin--4----C12-13 Phareth-9---4-4-PEG-40 Hydrogenated Castor Oil----4-4 Centella Asiatica Quantitative Extract 1111111 Purified Water To 100 To 100 To 100 To 100 To 100 To 100 To 100

[0071]

[0072] Experimental Example

[0073]

[0074] (1) Experimental Example 1: Evaluation of appearance and stability

[0075] Each of the solubilized compositions of the quantitative extract of *Cerambycodon grandiflorus* prepared in Examples 1 to 9 and Comparative Examples 1 to 14 was visually observed to evaluate their appearance and stability, and the results are shown in Table 5. In addition, photographs of each of the prepared Examples 1 to 9 and Comparative Examples 1 to 14 are shown in Figures 1 to 3.

[0076] Specifically, in the properties item, O was indicated if the properties were transparent or translucent, and X if they were opaque; in the stability item, O was indicated if no precipitation occurred, and X if precipitation occurred.

[0077]

[0078] Classification Properties Stability Example 1OO Example 2OO Example 3OO Example 4OO Example 5OO Example 6OO Example 7OO Example 8OO Example 9OO Comparative Example 1XX Comparative Example 2XX Comparative Example 3OX Comparative Example 4OO Comparative Example 5OX Comparative Example 6XX Comparative Example 7OX Comparative Example 8XX Comparative Example 9XX Comparative Example 10XX Comparative Example 11XX Comparative Example 12OX Comparative Example 13XX Comparative Example 14OX

[0079]

[0080] As a result of the experiment, in the case of Examples 1 to 9 and Comparative Example 4, a transparent or translucent formulation was formed and no precipitation phenomenon was detected, confirming that the quantitative extract of Centella asiatica was stably solubilized.

[0081] On the other hand, in Comparative Examples 1 to 3 and 5 to 14, a precipitation phenomenon occurred, and in Comparative Examples 1, 2, 6, 8 to 11 and 13, an opaque formulation appeared, confirming that the quantitative extract of Centella asiatica was not stably solubilized.

[0082]

[0083] (2) Experimental Example 2: Measurement of particle size and polydispersity index (PDI)

[0084] The average particle size and polydispersity index of each of the solubilized compositions of the quantitative extract of *Clematis florida* prepared in Examples 1 to 9 and Comparative Examples 1 to 14 were measured by performing particle size analysis using the ELSZneo device of Otsuka Korea, and the results are shown in Table 6.

[0085]

[0086] Classification Particle Size (nm) Polydispersity Index (PDI) Example 1 64.70.252 Example 2 107.10.244 Example 3 27.90.197 Example 4 72.00.190 Example 5 68.80.217 Example 6 115.20.147 Example 7 77.10.226 Example 8 29.80.166 Example 9 31.00.178 Comparative Example 1648.30.280 Comparative Example 2 157.50.277 Comparative Example 3 130.20.248 Comparative Example 4 140.20.288 Comparative Example 5 156.40.270 Comparative Example 6 725.60.273 Comparative Example 7144.60.286 Comparative Example 8832.20.276 Comparative Example 9892.60.297 Comparative Example 10833.70.279 Comparative Example 11675.40.276 Comparative Example 12176.50.277 Comparative Example 13658.70.251 Comparative Example 14195.60.269

[0087]

[0088] As a result of the experiment, it can be confirmed that Examples 1 to 9 exhibit an average particle size of 130 nm or less, indicating that the particles were solubilized to be sufficiently small. On the other hand, Comparative Examples 1 to 14 are confirmed to have an average particle size exceeding 130 nm, indicating that the particles were not solubilized to be sufficiently small.

[0089] In addition, it can be confirmed that Examples 1 to 9 exhibit a polydispersity index of 0.260 or less, indicating that the particles are uniformly distributed. On the other hand, Comparative Examples 1, 2, 4 to 12 and 14 are confirmed to have a polydispersity index exceeding 0.260, indicating that the particles are not uniformly distributed.

[0090]

[0091] Foregoing, specific parts of the contents of this application have been described in detail. It will be apparent to those skilled in the art that such specific descriptions are merely preferred embodiments and do not limit the scope of this application. Accordingly, the actual scope of this application is defined by the appended claims and their equivalents.

Claims

1. Contains titrated extract of Centella Asiatica (TECA), a solubilizing agent, and a solubilizing aid, and The above-mentioned solubilizing agent includes glycolipids, and A composition for solubilizing a quantitative extract of Centella asiatica, wherein the glycolipid comprises mannosylerythritol lipid and the solubilizing agent comprises dipotassium glycyrrhizate.

2. In Paragraph 1, A composition for solubilizing a quantitative extract of Centella asiatica, wherein the weight ratio of the above mannosylerythritol lipid and the above dipotassium glycyrrhizate is 1:0.5 to 7.

5.

3. In Paragraph 1, A composition for solubilizing a quantitative extract of Centella asiatica, comprising 0.2 to 10 weight percent of the above-mentioned mannosylerythritol lipid based on the total weight of the composition.

4. In Paragraph 1, A composition for solubilizing quantitative extract of Centella asiatica, comprising 1 to 15 weight percent of the above-mentioned dipotassium glycyrrhizate based on the total weight of the composition.

5. In Paragraph 1, A composition for solubilizing a quantitative extract of Centella asiatica, wherein the above-mentioned quantitative extract of Centella asiatica is included in an amount of 0.1 to 5 weight% relative to the total weight of the composition.

6. In Paragraph 1, A composition for solubilizing quantitative extract of Centella asiatica, having an average particle size of 130 nm or less as measured by the dynamic light scattering (DLS) method.

7. In Paragraph 1, A solubilized composition of quantitative extract of Centella asiatica, having a polydispersity index of 0.140 to 0.260 as measured by the dynamic light scattering (DLS) method.

8. A cosmetic composition comprising a solubilized composition of a quantitative extract of Centella asiatica according to any one of claims 1 to 7.

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