Seed treatment agent for promoting crop rooting, and preparation method therefor and use thereof
By preparing a seed dressing agent containing calcium dihydrogen phosphate, potassium dihydrogen phosphate, cooked fish meat, ferrous sulfate, magnesium sulfate, and compound probiotic liquid, the problem of poor root development during the seed germination to seedling rooting process of crops was solved, thereby improving crop growth and yield.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- HEILONGJIANG GREEN FOOD SCI RES INST
- Filing Date
- 2024-11-15
- Publication Date
- 2026-05-21
Smart Images
Figure PCTCN2024132345-FTAPPB-I100001 
Figure PCTCN2024132345-FTAPPB-I100002 
Figure PCTCN2024132345-FTAPPB-I100003
Abstract
Description
A seed dressing agent for promoting crop rooting, its preparation method and application Technical Field
[0001] This invention relates to the field of seed dressing technology, and more specifically to a seed dressing agent that promotes crop rooting, its preparation method, and its application. Background Technology
[0002] With social development and the improvement of people's living standards, people's demand for grain yield and quality is increasing. Poor root development during the process from seed germination to seedling rooting will affect the absorption and transport of nutrients and water by crops, resulting in stunted plants and easy lodging, which will seriously affect crop growth and yield.
[0003] During seed germination, seed dressing agents can externally supplement seeds with nutrients and physiologically active substances, promoting embryo development, seedling rooting, and crop growth. This is of great significance for improving crop yield and agricultural product quality, and for addressing the problems of decreasing arable land and slow agricultural output growth in my country.
[0004] In summary, how to provide a method for preparing a seed dressing agent that promotes crop rooting is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention
[0005] In view of this, the present invention provides a seed dressing agent for promoting crop rooting, its preparation method and application.
[0006] To achieve the above objectives, the present invention adopts the following technical solution:
[0007] This invention provides a seed dressing agent to promote crop rooting, which is prepared by mixing and fermenting the raw materials as follows: 10-20 parts of calcium dihydrogen phosphate, 5-10 parts of potassium dihydrogen phosphate, 5-10 parts of cooked fish meat, 2-4 parts of ferrous sulfate, 4-8 parts of magnesium sulfate, and 50-60 parts of compound probiotic liquid. The raw material components are mixed and fermented at a temperature above 4°C for 30 days to obtain the seed dressing agent to promote crop rooting.
[0008] A method for preparing a seed dressing agent that promotes crop rooting specifically includes the following steps:
[0009] (1) Preparation of compound probiotic solution: The bacterial solutions of Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii, and Saccharomyces cerevisiae were compounded according to the following weight ratio to obtain the compound probiotic solution. The amounts were: 25-40 parts Bacillus licheniformis bacterial solution, 20-35 parts Lactobacillus casei bacterial solution, 20-35 parts Lactobacillus delbrueckii bacterial solution, and 20-30 parts Saccharomyces cerevisiae bacterial solution.
[0010] (2) Obtaining the seed dressing agent: Weigh out 10-20 parts of calcium dihydrogen phosphate, 5-10 parts of potassium dihydrogen phosphate, 5-10 parts of cooked fish meat, 2-4 parts of ferrous sulfate, 4-8 parts of magnesium sulfate and 50-60 parts of compound probiotic liquid according to the predetermined weight ratio. Mix the raw material components and ferment them to obtain the seed dressing agent.
[0011] Furthermore, in step (2) above, the fermentation temperature is above 4°C and the facultative anaerobic fermentation lasts for more than 30 days.
[0012] The present invention also claims protection for the application of a seed dressing agent for promoting crop rooting as described above, or a seed dressing agent for promoting crop rooting prepared by the preparation method described above, in crop cultivation.
[0013] As can be seen from the above technical solution, compared with the prior art, the beneficial effects achieved by the present invention are: promoting embryo development and seedling rooting, promoting crop growth, and increasing crop yield. Detailed Implementation
[0014] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0015] The reagents required for this invention are conventional experimental reagents, purchased from commercially available channels; the experimental methods not mentioned are conventional experimental methods, and will not be described in detail here.
[0016] Example 1
[0017] Preparation of Seed Dressing Agent 1
[0018] The seed dressing agent comprises, by weight, 10 parts calcium dihydrogen phosphate, 9 parts potassium dihydrogen phosphate, 10 parts cooked fish meat, 3 parts ferrous sulfate, 8 parts magnesium sulfate, and 60 parts compound probiotic liquid. The compound probiotic is a mixture of bacterial liquids from Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii, and Saccharomyces cerevisiae. The weight percentages of the four bacterial liquids are 25 parts Bacillus licheniformis, 30 parts Lactobacillus casei, 35 parts Lactobacillus delbrueckii, and 27 parts Saccharomyces cerevisiae. The bacterial counts of the four bacterial liquids are as follows: Bacillus licheniformis liquid ≥ 1.5 × 10⁻⁶. 10 CFU / mL, Lactobacillus casei bacterial culture concentration ≥5.0×10⁻⁶ 11 cfu / mL, Lactobacillus delbrueckii bacterial culture with a bacterial count ≥4.0×10⁻⁶ 11 CFU / mL, the bacterial count of *Saccharomyces cerevisiae* in the culture is ≥5.0 × 10⁻⁶. 8 cfu / mL.
[0019] Add calcium dihydrogen phosphate, potassium dihydrogen phosphate, cooked fish meat, ferrous sulfate, magnesium sulfate and compound probiotic liquid together, stir and mix for fermentation. Fermentation temperature is above 4℃, and facultative fermentation is carried out for 33 days. The product after fermentation is used as seed dressing agent 1.
[0020] Example 2
[0021] Preparation of Seed Dressing Agent 2
[0022] The seed dressing agent comprises, by weight, 13 parts calcium dihydrogen phosphate, 10 parts potassium dihydrogen phosphate, 8 parts cooked fish meat, 4 parts ferrous sulfate, 7 parts magnesium sulfate, and 56 parts compound probiotic liquid. The compound probiotic is a mixture of bacterial liquids from Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii, and Saccharomyces cerevisiae. The weight percentages of the four bacterial liquids are 31 parts Bacillus licheniformis, 35 parts Lactobacillus casei, 20 parts Lactobacillus delbrueckii, and 23 parts Saccharomyces cerevisiae. The bacterial counts of the four bacterial liquids are as follows: Bacillus licheniformis liquid ≥ 1.5 × 10⁻⁶. 10 CFU / mL, Lactobacillus casei bacterial culture concentration ≥5.0×10⁻⁶ 11 cfu / mL, Lactobacillus delbrueckii bacterial culture with a bacterial count ≥4.0×10⁻⁶ 11 CFU / mL, the bacterial count of *Saccharomyces cerevisiae* in the culture is ≥5.0 × 10⁻⁶. 8 cfu / mL.
[0023] Add calcium dihydrogen phosphate, potassium dihydrogen phosphate, cooked fish meat, ferrous sulfate, magnesium sulfate and compound probiotic liquid together, stir and mix, ferment at a temperature above 4℃ for 35 days, and use the product after fermentation as seed dressing agent 2.
[0024] Example 3
[0025] Preparation of Seed Dressing Agent 3
[0026] The seed dressing agent comprises, by weight, 17 parts calcium dihydrogen phosphate, 7 parts potassium dihydrogen phosphate, 5 parts cooked fish meat, 3 parts ferrous sulfate, 6 parts magnesium sulfate, and 54 parts compound probiotic liquid. The compound probiotic is a mixture of bacterial liquids from Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii, and Saccharomyces cerevisiae. The weight percentages of the four bacterial liquids are 37 parts Bacillus licheniformis, 25 parts Lactobacillus casei, 30 parts Lactobacillus delbrueckii, and 20 parts Saccharomyces cerevisiae. The bacterial counts of the four bacterial liquids are as follows: Bacillus licheniformis liquid ≥ 1.5 × 10⁻⁶. 10 CFU / mL, Lactobacillus casei bacterial culture concentration ≥5.0×10⁻⁶ 11 cfu / mL, Lactobacillus delbrueckii bacterial culture with a bacterial count ≥4.0×10⁻⁶ 11 CFU / mL, the bacterial count of *Saccharomyces cerevisiae* in the culture is ≥5.0 × 10⁻⁶. 8 cfu / mL.
[0027] Add calcium dihydrogen phosphate, potassium dihydrogen phosphate, cooked fish meat, ferrous sulfate, magnesium sulfate and compound probiotic liquid together, stir and mix for fermentation. The fermentation temperature is above 4℃, and the facultative fermentation is carried out for 30 days. The product after fermentation is used as seed dressing agent 3.
[0028] Example 4
[0029] Preparation of Seed Dressing Agent 4
[0030] The seed dressing agent comprises, by weight, 20 parts calcium dihydrogen phosphate, 5 parts potassium dihydrogen phosphate, 7 parts cooked fish meat, 2 parts ferrous sulfate, 4 parts magnesium sulfate, and 50 parts compound probiotic liquid. The compound probiotic is a mixture of bacterial liquids from Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii, and Saccharomyces cerevisiae. The weight percentages of the four bacterial liquids are 40 parts Bacillus licheniformis, 20 parts Lactobacillus casei, 25 parts Lactobacillus delbrueckii, and 30 parts Saccharomyces cerevisiae. The bacterial counts of the four bacterial liquids are as follows: Bacillus licheniformis liquid ≥ 1.5 × 10⁻⁶. 10 CFU / mL, Lactobacillus casei bacterial culture concentration ≥5.0×10⁻⁶ 11 cfu / mL, Lactobacillus delbrueckii bacterial culture with a bacterial count ≥4.0×10⁻⁶ 11 CFU / mL, the bacterial count of *Saccharomyces cerevisiae* in the culture is ≥5.0 × 10⁻⁶. 8 cfu / mL.
[0031] Add calcium dihydrogen phosphate, potassium dihydrogen phosphate, cooked fish meat, ferrous sulfate, magnesium sulfate and compound probiotic liquid together, stir and mix for fermentation. Fermentation temperature is above 4℃, facultative fermentation for 37 days, and the product after fermentation is used as seed dressing agent 4.
[0032] Example 5
[0033] The effect of seed dressing agents on maize growth
[0034] The experimental variety was Zhengdan 958. 750 seeds were randomly divided into 5 groups: 4 experimental groups and 1 control group. In the experimental groups, 0.26 mL of seed dressing agent was diluted with water to 26 mL. Then, 150 corn seeds were mixed thoroughly with the diluted seed dressing agent, dried, and sown in germination boxes. In the control group, an equal volume of water was used instead of the diluted solution.
[0035] Germination experiments were conducted at 25℃. Germination potential, germination rate, seedling height, and root dry weight were measured. The average germination potential, average germination rate, average seedling height, and average root dry weight of each group were calculated. The results are shown in Table 1.
[0036] Table 1
[0037] The four groups of maize seeds treated with the seed dressing agent showed significantly higher germination potential, germination rate, seedling height, and root dry weight compared to the control group. This indicates that the seed dressing agent prepared above can significantly promote the germination of maize seeds.
[0038] The basic physicochemical properties of the field soil were: pH 7.46, topsoil organic matter content 26.5 g / kg, available phosphorus content 28 mg / kg, available potassium content 185 mg / kg, and available nitrogen content 126 mg / kg. The experiment was designed with 5 groups, including 4 experimental groups and 1 control group.
[0039] In the experimental group, the seed dressing agent was sprayed evenly onto the surface of corn seeds at a ratio of 0.5L seed dressing agent to 100kg of seeds. After mixing thoroughly, the seeds were exposed to the sun for 12 hours before sowing. The control group used an equal amount of water instead of seed dressing agent.
[0040] After corn emergence, the number of seedlings, field emergence rate, plant height, and number of roots were recorded. The average plant height, average emergence rate, and average number of roots for each group were calculated. The results are shown in Table 2.
[0041] Table 2
[0042] The four groups of maize treated with the seed dressing agent showed significantly higher plant height, emergence rate, and root number than the control group. This indicates that the seed dressing agents prepared above can significantly promote rooting and growth of maize.
[0043] Before corn harvest, indoor seed testing was conducted to calculate the average ear length, average ear diameter, average ear grain weight, and average 100-grain weight for each group. After corn harvest, the yield of each area was tallied, and the average yield of each area was calculated. The results are shown in Table 3.
[0044] Table 3
[0045] The four groups of maize treated with seed dressing agents showed significantly higher results than the control group in five traits: ear length, ear diameter, ear grain weight, 100-grain weight, and yield. The seed dressing agents prepared above can significantly promote maize growth and increase maize yield.
[0046] Example 6
[0047] Effects of seed dressing agents on winter wheat growth
[0048] The basic physicochemical properties of the field soil were: pH 8.32, topsoil organic matter content 12.5 g / kg, available phosphorus 10.88 mg / kg, available potassium 128.7 mg / kg, and total nitrogen 0.91 g / kg. The experiment was designed with 5 groups, including 4 experimental groups and 1 control group.
[0049] The experimental variety was Bainong 207, and the yield was 150 kg / hm². 2 Sowing was carried out at the appropriate rate. In the experimental group, the seed dressing agent was sprayed evenly onto the surface of the winter wheat seeds at a ratio of 0.75 L of seed dressing agent to 100 kg of seeds. After mixing thoroughly, the seeds were exposed to sunlight for 12 hours before sowing. In the control group, an equal amount of water was used instead of the seed dressing agent.
[0050] The number of seedlings was measured 15 days after winter wheat sowing, and plant height, root length, and number of roots per plant were measured 20 days after emergence. After winter wheat harvest, the yield of each area was tallied. The average emergence rate, average plant height, average root length, average number of roots, and average yield of each area were calculated. The results are shown in Table 4.
[0051] Table 4
[0052] The four groups of winter wheat treated with seed dressing agents showed significantly higher emergence rate, plant height, root length, number of roots, and yield than the control group. The seed dressing agents prepared above can significantly promote the rooting and growth of winter wheat and increase its yield.
[0053] Example 7
[0054] The effect of seed dressing agents on rice growth
[0055] The basic physicochemical properties of the field soil were: pH 6.52, topsoil organic matter content 42.5 g / kg, available phosphorus content 12.15 mg / kg, available potassium content 157.3 mg / kg, available nitrogen content 193.4 mg / kg, and total nitrogen content 0.28 g / kg. The experiment was designed with 5 groups, including 4 experimental groups and 1 control group.
[0056] The experimental variety was Longjing 26, and the yield was 60 kg / hm². 2Sowing was carried out at the appropriate rate. In the experimental group, the seed dressing agent was sprayed evenly onto the surface of the rice seeds at a ratio of 1.0 L of seed dressing agent to 100 kg of seeds. After mixing thoroughly, the seeds were exposed to sunlight for 12 hours before sowing. In the control group, an equal amount of water was used instead of the seed dressing agent.
[0057] A seedling quality survey was conducted 40 days after rice sowing, and plant height, stem base width, root length, and number of roots per plant were recorded. The average plant height, average stem base width, average root length, and average number of roots per plant were calculated for each group. The results are shown in Table 5.
[0058] Table 5
[0059] The rice seedlings treated with the seed dressing agent in the four groups showed significantly higher plant height, stem base width, root length, and root number compared to the control group. This indicates that the seed dressing agent prepared above can significantly promote rooting and growth of rice seedlings.
[0060] Before rice harvest, indoor seed testing was conducted to calculate the average number of panicles, average seed setting rate, and average thousand-grain weight for each group. After rice harvest, the yield of each area was tallied, and the average yield of each area was calculated. The results are shown in Table 6.
[0061] Table 6
[0062] The four groups of rice treated with the seed dressing agent showed significantly higher panicle number, seed setting rate, thousand-grain weight, and yield compared to the control group. This indicates that the seed dressing agent prepared above can significantly promote rice growth and increase rice yield.
[0063] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made based on the content of the present invention specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.
Claims
1. A seed dressing for promoting rooting of a crop, characterized by, The ingredients include the following parts by weight: 10-20 parts calcium dihydrogen phosphate, 5-10 parts potassium dihydrogen phosphate, 5-10 parts cooked fish meat, 2-4 parts ferrous sulfate, 4-8 parts magnesium sulfate, and 50-60 parts compound probiotic liquid.
2. The method of producing the seed dressing for promoting rooting of crops according to claim 1, characterized by, Specifically, the following steps are included: (1) Preparation of compound probiotic liquid; (2) Obtaining the seed dressing agent.
3. The method for preparing a seed dressing agent to promote crop rooting according to claim 2, characterized in that, In step (1), the compound probiotics include Bacillus licheniformis, Lactobacillus casei, Lactobacillus delbrueckii and Saccharomyces cerevisiae; in step (2), the fermentation temperature is above 4°C, the fermentation conditions are facultative anaerobic fermentation, and the fermentation time is above 30 days.
4. The application of a seed dressing agent for promoting crop rooting as described in claim 1 or a seed dressing agent for promoting crop rooting prepared by the preparation method described in claims 2-3 in crop cultivation.