Methods for detecting extracellular vesicle-associated molecules

WO2026107359A3PCT designated stage Publication Date: 2026-06-25GUARDANT HEALTH INC
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
GUARDANT HEALTH INC
Filing Date
2025-11-14
Publication Date
2026-06-25

AI Technical Summary

Technical Problem

Existing methods for analyzing extracellular vesicle (EV)-associated molecules in liquid biopsies, such as blood samples, are inadequate for sensitive and accurate detection due to low and variable amounts of cancer-associated molecules, leading to obscured predictive value of mutations and challenges in isolating and processing EVs for analysis.

Method used

A method involving partitioning EVs into compartments to ensure single EVs per compartment, followed by detection of EV-associated target molecules using nucleic acid sequencing or amplification, optionally with binding agent-oligonucleotide conjugates, to analyze EV-associated proteins and nucleic acids.

Benefits of technology

Enables sensitive and accurate detection of EV-associated molecules, allowing for improved understanding of heterogeneous EV populations and earlier disease detection, particularly in cancer diagnosis.

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Abstract

Provided herein are methods of analyzing a plurality of extracellular vesicle (EV)-associated target molecules in a sample. An exemplary method comprises (a) partitioning extracellular vesicles in the sample into a plurality of compartments, wherein after the partitioning, only a single extracellular vesicle is present in at least a portion of the plurality of compartments, thereby providing partitioned extracellular vesicles; and (b) for at least a portion of the partitioned extracellular vesicles, detecting a presence, absence, or level of each of the plurality of EV-associated target molecules using nucleic acid sequencing.
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