Aerosol test device

Abstract

An embodiment of the present invention provides an aerosol test device comprising: a lower frame comprising a chip seating portion on which a chip including a sample is seated; and a fluid inlet / outlet which is mounted on the upper side of the lower frame and supplies, to the sample, and discharges a fluid, wherein the fluid inlet / outlet comprises: a body; a fluid entrance comprising an inflow tube which is connected to the body and extends toward the seating portion; a contact portion comprising an end portion that comes into contact with the chip and a contact tube surrounding the inflow tube; and a fluid outlet portion which is connected to the body and is connected to the contact tube. In addition, the fluid inlet / outlet comprises a moving portion which moves the inflow tube in the direction in which the inflow tube extends.

Description

Aerosol testing device

[0001] The present invention relates to an aerosol testing device.

[0002] As part of a health assessment, the biochemical effects of airborne substances and gas mixtures (referred to as 'aerosols') on cells, for example, the respiratory systems of humans or animals, are investigated. This can be done by placing biological samples on a membrane and exposing them to aerosols.

[0003] Conventionally, a device is known for placing a sample in an exposure chamber and exposing the sample to an aerosol. After placing the sample, such a device exposes the sample to an aerosol and monitors changes in the sample after a period of time.

[0004] (Patent Document 1) European Patent Publication No. 3287762 (Published Feb. 28, 2018)

[0005] The present invention aims to provide an aerosol testing device capable of controlling fluid flow to a sample.

[0006] The present invention aims to provide an aerosol testing device capable of simulating respiratory positions by adjusting the Stokes number of the fluid flow.

[0007] To achieve the above objectives, the present invention may provide the following aerosol testing device.

[0008] The present invention provides an aerosol testing device comprising, in one embodiment, a lower frame including a chip mounting portion on which a chip including a sample is mounted; and a fluid inlet / outlet portion mounted on the upper side of the lower frame and supplying and discharging fluid to the sample; wherein the fluid inlet / outlet portion includes a body, a fluid inlet portion including an inlet pipe connected to the body and extending toward the chip mounting portion, a contact portion including an end portion contacting the chip and a contact pipe surrounding the inlet pipe, and a fluid outlet portion connected to the body and connected to the contact pipe, and wherein the fluid inlet / outlet portion includes a moving portion that moves the inlet pipe in the direction of extension of the inlet pipe.

[0009] In one embodiment, the moving part may be partially fixed to the body.

[0010] In one embodiment, the moving part may include a first part connected to the fluid inlet part and moving together, and a second part connected to the first part through a screw thread and fixed to the body, and the inlet pipe may be configured to advance and retract in the extension direction by rotating the first part relative to the second part.

[0011] In one embodiment, the first part of the moving part and the contact part may be integrally formed with the body.

[0012] In one embodiment, the first part is coupled to the fluid inlet part, and the moving part may surround and be coupled to the first part and include a scale formed on the outer surface.

[0013] In one embodiment, the contact portion includes a first seal ring that contacts the chip at a location different from the sample at the lower part of the end, and the first portion is inserted into an inlet groove of the body, and a second seal ring that adheres to the inlet groove may be mounted.

[0014] In one embodiment, the present invention provides an aerosol testing device comprising: a lower frame on which a chip including a sample is placed; and a fluid inlet / outlet unit mounted on the upper side of the lower frame and supplying and discharging fluid to and from the sample, wherein the fluid inlet / outlet unit comprises a body having an internal space formed to cover the chip, a fluid inlet unit including an inlet pipe connected to the body and extending toward the sample, a fluid outlet unit connected to the body and connected to the internal space, and a moving unit that moves the inlet pipe in the direction of extension of the inlet pipe, and wherein the moving unit is disposed outside the body.

[0015] In one embodiment, the moving part includes a fixed part fixedly coupled to the outside of the body and a movable part that moves relative to the fixed part and is coupled to the fluid inlet part, and the movable part and the fixed part may be connected by a gear or a screw.

[0016] In one embodiment, the movable part is coupled to the fluid inlet part, and the body includes an inlet groove into which the fluid inlet part is inserted and an outlet groove into which the fluid outlet part is inserted, and the internal space has a bell shape communicating with the inlet groove and the outlet groove, the lower part of the internal space is covered by the lower frame, and the inlet pipe can extend into the interior of the internal space.

[0017] In one embodiment, the moving part includes a fixed part fixedly coupled to the outside of the body and a movable part that moves relative to the fixed part and is connected to the inlet pipe, and the movable part may include an extension part coupled to the inlet pipe and a rotatable operating part to move the movable part relative to the fixed part.

[0018] In one embodiment, the operating part may be a micrometer in which the anvil is connected to the fixed part and the spindle is connected to the movable part.

[0019]

[0020] In one embodiment, the lower frame includes a plurality of chip mounting portions on which the chip is mounted, and the fluid inlet / outlet portion may include a plurality of movable portions to individually control a plurality of internal spaces and an inlet pipe extending into the internal spaces corresponding to the plurality of chip mounting portions.

[0021] In one embodiment, a seal ring that adheres to the inlet groove of the body may be disposed on the lower outer surface of the fluid inlet portion.

[0022] The present invention aims to provide an aerosol testing device capable of controlling fluid flow to a sample through the above configuration.

[0023] The present invention aims to provide an aerosol testing device capable of simulating respiratory positions by adjusting the Stokes number.

[0024] Figure 1 is a graph showing the deposition rate according to particle diameter by respiratory region.

[0025] Figure 2 is a schematic diagram of a conventional aerosol test apparatus.

[0026] Figure 3 is a schematic diagram of an inertial collider.

[0027] FIG. 4 is a perspective view of an aerosol test device according to an embodiment of the present invention.

[0028] FIG. 5 is another perspective view of an aerosol test device according to one embodiment of the present invention.

[0029] FIG. 6 is an exploded perspective view of an aerosol test device according to an embodiment of the present invention.

[0030] FIG. 7 is a cross-sectional view of an aerosol test device according to an embodiment of the present invention.

[0031] FIG. 8 is a perspective view of an aerosol test device according to another embodiment of the present invention.

[0032] FIG. 9 is a partial cross-sectional view of an aerosol test device according to another embodiment of the present invention.

[0033] Figure 10 is a partial cross-sectional view showing the movement of the inlet pipe in Figure 9.

[0034] * Explanation of the symbols *

[0035] 100: Aerosol test device 110: Base

[0036] 120: Lower plate 121: Chip settling area

[0037] 122: Fastening groove 123: Top surface

[0038] 200: Fluid inlet / outlet 201: Body

[0039] 202: Fastening groove 206: Internal space

[0040] 210: Fluid inlet 211: Inlet pipe

[0041] 220: Fluid outlet 221: Outlet pipe

[0042] 230: Contact part 232: Contact tube

[0043] 233: Seal Ring 250: Moving Part

[0044] 251: Part 1 252: Part 2

[0045] 253: Scale part 255: Fixed part

[0046] 256: Moving part 257: Control part

[0047] Preferred embodiments of the present invention will be described below with reference to the attached drawings. However, embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

[0048] In addition, embodiments of the present invention are provided to more fully explain the present invention to those with average knowledge in the relevant technical field.

[0049] In drawings, the shapes and sizes of elements may be exaggerated for clearer explanation.

[0050] In describing the embodiments of the present invention, if it is determined that a detailed description of known technology related to the present invention may unnecessarily obscure the essence of the present invention, such detailed description will be omitted. Furthermore, the terms described below are defined considering their functions in the present invention, and these may vary depending on the intentions or conventions of the user or operator. Therefore, such definitions should be based on the content throughout this specification. The terms used in the detailed description are merely for describing the embodiments of the present invention and should not be limited in any way. Unless explicitly stated otherwise, expressions in the singular form include the meaning of the plural form.

[0051] In this description, expressions such as “include” or “equipped” are intended to refer to certain characteristics, numbers, steps, actions, elements, parts or combinations thereof, and should not be interpreted to exclude the existence or possibility of one or more other characteristics, numbers, steps, actions, elements, parts or combinations thereof other than those described.

[0052] In the specification, terms such as 'top', 'upper', 'upper surface', 'lower', 'lower', 'lower surface', and 'side' are based on the drawings and may actually vary depending on the orientation in which the elements or components are arranged.

[0053] Additionally, throughout the specification, when it is said that one part is 'connected' to another part, this includes not only cases where they are 'directly connected,' but also cases where they are 'indirectly connected' with other elements in between.

[0054] The present invention will be described in detail below through each embodiment or example of the invention. It should be noted that each embodiment or example described in this specification is not limited to a single embodiment or example, but may also be combined with other embodiments or examples. Accordingly, the citation of claims in the patent claims is merely an example of an embodiment, and the technical concept of the present invention should not be interpreted as being limited only to a combination with the cited claims; rather, combinations with various claims are also included within the scope of the technical concept of the present invention.

[0055] Figure 1 shows a graph illustrating the deposition rate according to particle diameter for each respiratory region. As shown in Figure 1, the size and proportion of deposited particles vary depending on the location of the respiratory region; therefore, for accurate testing of aerosols, it is necessary to simulate the location to be tested.

[0056] Figure 2 shows two schematic diagrams of an aerosol test apparatus. In the apparatus of Figure 2(a), an aerosol is supplied through an inlet (I), passes over the upper part of a sample (C), for example, a biological tissue sample, placed in a container (V), and is discharged through an outlet (O). In the apparatus of Figure 2(b), the inlet (I) of the aerosol is positioned above the sample (C) placed inside the container (V), and the aerosol supplied from the inlet is diverted inside the container (V) and then discharged to the outside.

[0057] Since this test device performs tests at fixed positions for the inlet (I), outlet (O), and container (V), it is impossible to simulate the positional characteristics of the respirator. To improve this, methods have been proposed to simulate positional characteristics by applying an electromagnetic field to the test device or by providing moisture to utilize electromagnetic force or gravity; however, these methods have limitations in that they can affect the sample (C) and cannot provide accurate simulation because moisture or electromagnetic force is not provided in the actual respirator.

[0058] Meanwhile, Figure 3 shows a schematic diagram of an inertial collider in a single nozzle structure. In this inertial collider, the ratio (S / W) of the nozzle width (W) to the distance (S) to the collision plate sharpens the collection efficiency curve.

[0059] In one embodiment, the present invention provides an aerosol testing device that enables the simulation of respiratory locations by changing the ratio of particles contained in the aerosol. By changing the ratio, the Stokes Number changes, and since the size of the particles primarily captured changes when the Stokes Number changes, the simulation of respiratory locations can be made more accurate. Furthermore, in one embodiment of the present invention, since the respiratory system is simulated solely by mechanical configuration, factors unrelated to reality, such as moisture and electromagnetic interference, can be excluded, thereby enabling accurate aerosol testing.

[0060] In one embodiment of the present invention, the distance (S) is adjusted by controlling the distance between a sample corresponding to the impact surface and an aerosol supply pipe corresponding to the nozzle, thereby enabling the performance of tests corresponding to various respiratory positions.

[0061] FIGS. 4 to 7 illustrate an aerosol testing device according to an embodiment of the present invention. FIG. 4 illustrates a perspective view of an aerosol testing device according to an embodiment of the present invention, FIG. 5 illustrates another perspective view of an aerosol testing device according to an embodiment of the present invention, FIG. 6 illustrates an exploded perspective view of an aerosol testing device according to an embodiment of the present invention, and FIG. 7 illustrates a cross-sectional view of an aerosol testing device according to an embodiment of the present invention.

[0062] In this embodiment, the aerosol test device (100) includes a base (110), a lower frame (120), and a fluid inlet / outlet part (200) coupled to the lower frame (120).

[0063] The base (110) is where the lower frame (120) is placed and has a flat upper plane.

[0064] The lower frame (120) includes a chip mounting portion (121) on which a chip (300) is mounted, a fastening groove (122) formed on the upper surface (123), and an observation groove (124) formed on a part of the chip mounting portion (121). The chip mounting portion (121) is formed in a groove shape from the upper surface (123) in a shape corresponding to the chip (300), and a plurality of them are arranged in two rows. In this embodiment, a total of 12 chip mounting portions (121) are arranged in two rows of six each, but are not limited thereto, and one or more are sufficient. The chip mounting portions (121) are connected to each other. A plurality of fastening grooves (122) are arranged on the upper surface (123) surrounding the chip mounting portion (121). A bolt (B) penetrating the fluid inlet / outlet section (200) is fastened to the fastening groove (122), and the contact portion (230) of the fluid inlet / outlet section (200) is in close contact with the chip (300) by fastening the bolt (B). An observation groove (124) is formed penetrating the lower frame (120), and the observation groove (124) is positioned at a location corresponding to the sample placement groove (310) of the chip (300), so that the sample can be observed during or after the test without separating the fluid inlet / outlet section (200).

[0065] The fluid inlet / outlet section (200) includes a body (201), a fluid inlet section (210) including an inlet pipe (211) connected to the body (201) and extending toward the chip seating section (121), a contact section (230) including an end that contacts the chip (300) and a contact pipe (232) surrounding the inlet pipe (211), and a fluid outlet section (220) connected to the body (201) and connected to the contact pipe (232).

[0066] The fluid inlet / outlet section (200) may be provided with a plurality of channels corresponding to the chip mounting section (121). That is, the fluid inlet / outlet section (200) includes the first to Nth fluid inlet / outlet sections (200a, 200b, 200c, 200d, 200e). Since the first to Nth fluid inlet / outlet sections (200a, 200b, 200c, 200d, 200e) all have the same structure, they will be described as a single fluid inlet / outlet section (200). The number of fluid inlet / outlet sections (200) is the same as the number of chip mounting sections (121).

[0067] In this embodiment, the body (201) has a rectangular shape, but is not limited thereto and can have various shapes. The body (201) includes an inlet groove (203) through which an inlet pipe (211) passes at the top, an outlet groove (204) connected to an outlet pipe (221) at one side, an intersection part (205) where the inlet groove (203) and the outlet groove (204) intersect in the middle, and a fastening groove (202) formed through in the vertical direction at a position that does not overlap with the inlet groove (203) or the outlet groove (204), and a contact part (230) is integrally connected to the bottom. The aforementioned bolt (B) is inserted into the fastening groove (202) to fasten the body (201) and the lower frame (120). In this embodiment, the body (201) is implemented in a form in which the groove is formed from a single member, but is not limited thereto, and a method in which each component is implemented in a separate member and combined is also possible.

[0068] The fluid inlet section (210) includes an inlet pipe (211) and a ferrule (212) that fixes the inlet pipe (211) and connects it to the moving section (250). The inlet pipe (211) is configured to extend in a straight line inside the body (201), and the direction of extension is perpendicular to the upper surface (123) of the lower frame (120). That is, in this embodiment, the lower frame (120) has an upper surface parallel to the horizontal plane, and the inlet pipe (211) extends in a vertical direction.

[0069] The ferrule (212) can be configured so that the lower outer surface (213) is fitted into the upper part of the through groove (251a) of the first part (251) of the moving part (250) to be described later, and the first part (251) and the ferrule (212) move together at least in the up and down direction.

[0070] The fluid outlet section (220) includes an outlet pipe (221) and a ferrule (222) that fixes the outlet pipe (221) and is inserted into the outlet groove (204) of the body (201).

[0071] The contact portion (230) is implemented at the lower part of the body (201) and includes a contact tube (232) composed of a groove formed in the body (201), a protrusion (231) protruding downward in a tubular shape from the lower part of the contact tube (232), and a seal ring (233) coupled to the lower end of the protrusion (231). The contact tube (232) has a diameter larger than that of the inlet tube (211) and a diameter corresponding to that of the sample placement groove (310) of the chip (300) to be described later, thereby forming a path through which the aerosol passing through the inlet tube (211) changes direction at the sample placement groove (310) and exits to the outlet tube (221) without leakage.

[0072] The moving part (250) is configured to move the inlet pipe (211) in an upward and downward direction, which is the extension direction, and includes a first part (251) connected to the fluid inlet part (210) and moved together, a second part (252) connected to the first part (251) through screw threads (251c, 252a) and fixed to the body (201), and a scale part (253) surrounding the outside of the first part (251) and having a scale indicating the degree of movement.

[0073] The first part (251) is inserted into the lower outer surface (213) of the ferrule (212) and includes a through groove (251a) through which the inlet pipe (211) passes, an upper outer surface (251b) coupled with the scale portion (253) at the top, a thread (251c) formed at the bottom of the upper outer surface (251b) and coupled with the thread (252a) of the second part (252), and a seal ring (251d) provided on the outer surface and in close contact with the inner surface of the inlet groove (203) to prevent leakage through the inlet groove (203).

[0074] The second part (252) may be formed as a separate member, but in this embodiment, it is formed integrally with the body (201) constituting the inlet groove (203). The second part (252) has the inlet groove (203) extended on its inner surface and includes a thread (252a) that is coupled to the thread (251c) of the first part (251).

[0075] The scale portion (253) includes an inner portion (253b) that is coupled to the upper outer portion (251b) of the first portion (251) inside, and a scale (253a) on the outer surface that can indicate the degree of rotation to the user. The scale (253a) is provided at a position extending outward from the second portion (252), and the reference line of the scale (253a) can be displayed on the outside of the body (201). The scale portion (253) and the first portion (251) are coupled via a bolt (not shown), and can be configured so that the scale portion (253) and the first portion (251) move together at that position after the first portion (251) is moved to set the zero point.

[0076] In this embodiment, the moving part (250) converts the rotation of the scale part (253) into movement in the up and down direction of the inlet pipe (211) through the screw threads (251c, 252a), and the slope of the screw threads (251c, 252a) is formed gently to enable precise operation. However, the present invention is not limited to precise operation through screws, and it is obvious that it may be modified and implemented using gears or other mechanical mechanisms as long as the inlet pipe (211) can be precisely adjusted in the up and down direction.

[0077] Meanwhile, between the first part (251) and the ferrule (212), a member such as a bearing may be arranged to allow rotation in the rotational direction and to move together in the up and down direction.

[0078] The chip (300) is placed on the chip mounting portion (121), and the chip (300) is provided with a sample placement groove (310), so that a biological sample can be placed on the inner lower surface of the sample placement groove (310). A structure similar to an inertial collider as described in FIG. 3 can be formed by the sample placement groove (310) and the inlet pipe (211).

[0079] The operation of the aerosol test device (100) will be briefly explained. With the fluid inlet / outlet part (200) and the lower frame (120) separated, the aerosol test device (100) places a chip (300), which is placed in the sample placement groove (310), on the chip seating part of the lower frame (120).

[0080] Afterward, the chip (300) is covered through the fluid inlet / outlet section (200), and the fastening groove (202) of the body (201) and the fastening groove (122) of the lower frame (120) are joined using a bolt (B). At this time, the lower surface of the seal ring (233) of the contact section (230) of the lower part of the body (201) surrounds the sample placement groove (310) and comes into contact with the chip (300). Since the seal ring (233) is formed of an elastic material, the fastening torque of the bolt (B) is adjusted to a preset torque so that the seal ring (233) is pressed to a certain degree.

[0081] Meanwhile, the moving part (250) is configured to be adjustable based on when the inlet tube (211) touches the lower surface of the sample placement groove (310). That is, by adjusting the zero point of the scale part (253) based on when the inlet tube (211) touches the lower surface of the sample placement groove (310) through the scale part (253) of the moving part (250) and then operating the scale part (253), the distance of the inlet tube (211) from the sample placement groove (310) can be determined. This allows the distance from the inner surface of the sample placement groove (310), which is the distance (S) to the collision plate in FIG. 3, to the inlet tube (211) to be accurately adjusted through the scale part (253), thereby enabling position simulation in the respiratory system. It is preferable to perform this zero adjustment after placing the empty chip (300) before starting the actual test, but it is also possible to perform the zero adjustment again after placing the chip (300) for the actual test.

[0082] With the zero point adjusted, the scale portion (253) of the moving part (250) is operated so that the vertical distance between the inlet pipe (211) and the sample placement groove (310) corresponds to the position to be tested.

[0083] After the position of the inlet pipe (211) is set, the condition of the biological sample according to the aerosol is tested while supplying aerosol through an aerosol supply unit (not shown) connected to the inlet pipe (211).

[0084] The present invention enables the simultaneous testing of multiple biological samples and allows for obtaining test results from samples at multiple locations on the respiratory tract using a single test device. Since variations occur with each production of biological samples, samples produced once may possess identical properties, whereas subsequent samples may not. By allowing for the simultaneous testing of multiple biological samples at different locations, the present invention enables the accurate measurement of the effects of aerosols at different respiratory locations under identical conditions, thereby providing reliable results.

[0085] FIGS. 8 to 10 illustrate an aerosol test device (100) according to another embodiment of the present invention.

[0086] The aerosol test device (100) of this embodiment also includes a lower frame (120) on which a chip (300) is placed, and a fluid inlet / outlet part (200) connected to the lower frame (120) at the top of the lower frame (120).

[0087] The chip (300) is placed on the upper surface (123) of the lower frame (120), and if necessary, a chip placement portion may be provided to specify the placement position of the chip (300).

[0088] The above fluid inlet / outlet section (200) includes a body (201) having an internal space (206) that covers the chip (300), a fluid inlet section (210) including an inlet pipe (211) connected to the body (201) and extending toward the sample, a fluid outlet section (220) connected to the body (201) and connected to the internal space (206), and a moving section (250) that moves the inlet pipe (211) in the direction of extension of the inlet pipe (211).

[0089] The lower frame (120) includes a fastening groove (122) formed on the upper surface (123) on which the chip (300) is seated. If necessary, it may include a chip seating portion that defines the position of the chip (300) and on which the chip (300) is seated, similar to the embodiment of FIG. 4.

[0090] The fluid inlet / outlet section (200) includes a body (201) having an internal space (206), a fluid inlet section (210) connected to the body (201) and including an inlet pipe (211) extending toward a sample of the chip (300), and a fluid outlet section (220) connected to the body (201) and connected to the internal space (206).

[0091] In the body (201) of the fluid inlet / outlet section (200), a plurality of internal spaces (206) may be provided. In this embodiment, a structure is disclosed in which six internal spaces (206) are provided, but is not limited thereto. In this embodiment, a fluid inlet section (210), a fluid outlet section (220), and a moving section (250) are provided corresponding to the number of internal spaces (206).

[0092] In this embodiment, the body (201) has a rectangular shape, but is not limited thereto and can have various shapes. The body (201) includes an inlet groove (203) through which an inlet pipe (211) passes from the upper part of the inner space (206) to be connected to the inner space (206), an outlet groove (204) connected to an outlet pipe (221) on one side, and a fastening groove (202) formed through an outer protrusion that does not overlap with the inlet groove (203) or the outlet groove (204) in the vertical direction. A bolt (B) is inserted into the fastening groove (202) to fasten the body (201) to the lower frame (120).

[0093] The above internal space (206) has a structure in which the lower surface is open in the body (201), but the lower surface is covered by the lower frame (120) to form a single space. The internal space (206) has a bell shape in which the upper part is connected to an inlet groove (203) and the side part is connected to an outlet groove (204).

[0094] The fluid inlet section (210) includes an inlet pipe (211) and a ferrule (212) that fixes the inlet pipe (211). The inlet pipe (211) is configured to extend in a straight line inside the body (201), and the direction of extension is perpendicular to the upper surface (123) of the lower frame (120). That is, in this embodiment, the lower frame (120) has an upper surface parallel to the horizontal plane, and the inlet pipe (211) extends in a vertical direction.

[0095] The lower outer surface (213) of the ferrule (212) is fitted into the inlet groove (203), and a seal ring (215) is placed to seal the space between the outer surface (213) and the inlet groove (203). Multiple seal rings (215) may be placed along the extension direction, thereby allowing the ferrule (212) to move together with the inlet pipe (211) as it moves, while sealing the inlet groove (203) to prevent leakage through the inlet groove (203).

[0096] The fluid outlet section (220) includes an outlet pipe (221) and a ferrule (222) that secures the outlet pipe (221) and is inserted into the outlet groove (204) of the body (201). The outlet pipe (221) communicates with the internal space (206).

[0097] The moving part (250) is configured to move the inlet pipe (211) in an up-and-down direction, which is an extension direction, and is connected to the outside of the body (201). The moving part (250) includes a fixed part (255) fixedly coupled to the outside of the body (201), and a movable part (256) that moves relative to the fixed part (255) and is connected to the inlet pipe (211). The movable part (256) includes a spindle (256a) that moves up and down from the upper part of the fixed part (255), an extension part (256b) that extends from the spindle (256a) and is coupled to the inlet pipe (211), and an operating part (257) that moves the spindle (256a) relative to the fixed part (255). The operating part (257) may be a micrometer, and the anvil of the micrometer is connected to the fixed part (255), and the spindle (256a) is connected to the inlet pipe (211) so that the inlet pipe (211) can be moved precisely in response to the operation of the micrometer.

[0098] The chip (300) is seated on the upper surface (123) of the lower frame (120) and inside the internal space (206), and the chip (300) is provided with a sample placement groove (310), so that a biological sample can be placed on the inner lower surface of the sample placement groove (310). The inlet tube (211) is placed in the sample placement groove (310) so that a structure similar to an inertial collider as described in FIG. 3 can be formed.

[0099] The operation of the aerosol test device (100) according to this embodiment will be briefly described. The aerosol test device (100) may first perform zero point adjustment. With the inlet pipe (211) in contact with the lower frame (120) in the upper body where the lower frame (120) and the body (201) are combined, the position of the inlet pipe (211) separated from the upper surface of the lower frame (120) can be precisely adjusted through the operating part (257). After that, with the fluid inlet / outlet part (200) and the lower frame (120) separated, a chip (300) is placed on the lower frame (120).

[0100] After that, the chip (300) is covered through the fluid inlet / outlet section (200), and the fastening groove (202) of the body (201) and the fastening groove (122) of the lower frame (120) are joined with a bolt (B). By adjusting the distance of the inlet pipe (211) from the lower frame (120) through a micrometer, the vertical distance between the sample placement groove (310) and the inlet pipe (211) can be precisely adjusted, thereby enabling position simulation in the respiratory system.

[0101] After the position of the inlet pipe (211) is set in this way, the condition of the biological sample according to the aerosol is tested while supplying aerosol through an aerosol supply unit (not shown) connected to the inlet pipe (211).

[0102] This testing method enables the solution to the problem where accurate testing cannot be performed due to errors or tolerances that occur during the manufacturing of parts. That is, since zero point adjustment is performed while reflecting the tolerances and errors that occur during manufacturing, the vertical distance between the inlet tube (211) and the sample placement groove (310) can be accurately adjusted, and by accurately adjusting this vertical distance, the position in the breathing apparatus can be accurately simulated.

[0103] Although the present invention has been described above with reference to embodiments, it is understood that the invention is not limited thereto and can be implemented with various modifications.

Claims

1. A lower frame including a chip mounting portion on which a chip containing a sample is mounted; and A fluid inlet / outlet part mounted on the upper side of the lower frame and supplying and discharging fluid to the sample; comprising, The above fluid inlet / outlet section comprises a body, a fluid inlet section including an inlet pipe connected to the body and extending toward the seating section, a contact section including an end section in contact with the chip and a contact pipe surrounding the inlet pipe, and a fluid outlet section connected to the body and connected to the contact pipe. The above fluid inlet / outlet section is an aerosol test device comprising a moving section that moves the inlet pipe in the direction of extension of the inlet pipe.

2. In Paragraph 1, The above-mentioned moving part is an aerosol testing device in which a portion is fixed to the above-mentioned body.

3. In Paragraph 1, The above-mentioned moving part includes a first part connected to the fluid inlet part and moving together, and a second part connected to the first part through screw threads and fixed to the body. An aerosol test device configured such that the inlet pipe advances and retracts in an extension direction by rotating the first part relative to the second part.

4. In Paragraph 3, An aerosol testing device in which the first part of the moving part and the contact part are integrally formed in the body.

5. In Paragraph 3, The above first part is coupled to the fluid inlet, and An aerosol testing device comprising a moving part that surrounds and is coupled to the first part and includes a scale formed on the outer surface.

6. In Paragraph 5, The above contact portion includes a first seal ring that contacts the chip at a position different from the sample at the lower part of the above end, and The above-mentioned first part is inserted into the inlet groove of the body, and an aerosol testing device is equipped with a second seal ring that adheres to the inlet groove.

7. A lower frame on which a chip containing a sample is seated; and A fluid inlet / outlet part mounted on the upper side of the lower frame and supplying and discharging fluid to the sample; comprising, The above fluid inlet / outlet section includes a body having an internal space that covers the chip, a fluid inlet section including an inlet pipe connected to the body and extending toward the sample, a fluid outlet section connected to the body and connected to the internal space, and a moving section that moves the inlet pipe in the direction of extension of the inlet pipe. The above moving part is an aerosol testing device positioned outside the body.

8. In Paragraph 7, The above moving part includes a fixed part fixedly coupled to the outside of the body and a movable part that moves relative to the fixed part and is coupled to the fluid inlet part. The above-mentioned movable and fixed parts are connected by a gear or a screw in an aerosol testing device.

9. In Paragraph 8, The above movable part is coupled to the above fluid inlet part, and The above body includes an inlet groove into which the fluid inlet portion is inserted and an outlet groove into which the fluid outlet portion is inserted, and The above internal space has a bell shape communicating with the above inlet groove and outlet groove, and The lower part of the above internal space is covered by the lower frame, and The above-mentioned inlet pipe is an aerosol testing device that extends into the interior of the above-mentioned internal space.

10. In Paragraph 7, The above-mentioned moving part includes a fixed part fixedly coupled to the outside of the body, and a movable part that moves relative to the fixed part and is connected to the inlet pipe. The above-mentioned movable part is an aerosol test device comprising an extension part connected to the inlet pipe and a rotatable operating part for moving the movable part relative to the fixed part.

11. In Paragraph 10, The above-described operating part is an aerosol testing device in which an anvil is connected to a fixed part and a spindle is connected to a movable part, which is a micrometer.

12. In Paragraph 7, The lower frame includes a plurality of mounting portions on which the chip is seated, and The above fluid inlet / outlet section is an aerosol testing device comprising a plurality of moving parts to individually control a plurality of internal spaces corresponding to the plurality of seating sections and an inlet pipe extending into the internal spaces.

13. In Paragraph 7, An aerosol testing device having a seal ring disposed on the lower outer surface of the fluid inlet portion that is in close contact with the inlet groove of the body.

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