Silk fibroin gel dressing, preparation method therefor, and use thereof
The preparation of silk fibroin gel dressings solves the problems of dryness and adhesion of traditional dressings, providing easy application, absorption, safety, and gentle multi-dimensional protection, and promoting wound healing.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- SHANGHAI REGE-INNOVATION MEDICAL TECHNOLOGY CO LTD
- Filing Date
- 2025-04-11
- Publication Date
- 2026-07-02
AI Technical Summary
Traditional wound dressings have limitations such as drying out and causing delayed healing, and the material adhering to the wound surface and causing secondary damage. In addition, existing silk fibroin dressings do not cover large wounds completely, are easy to rub off, are difficult to clean, and may contain irritating ingredients.
The silk fibroin gel dressing formula contains silk fibroin, inducers, protectants and thickeners. Through specific ratios and preparation methods, it forms a multi-dimensional protective layer that is easy to apply and absorb, gentle and painless, and promotes wound healing.
It is easy to apply and absorb, requires no rinsing, uses safe ingredients, provides multi-dimensional protection and repair of the skin barrier, relieves postoperative discomfort, and promotes wound healing.
Smart Images
Figure CN2025088606_02072026_PF_FP_ABST
Abstract
Description
A silk fibroin gel dressing, its preparation method and application Technical Field
[0001] This invention relates to the field of silk fibroin application technology, and in particular to a silk fibroin gel dressing, its preparation method and application. Background Technology
[0002] Traditional wound dressings have long relied on passive protective mechanisms such as physical isolation and exudate absorption. These mechanisms have limitations, including delayed healing due to dryness and secondary damage caused by material adhesion to the wound surface, particularly in the management of chronic wounds. In recent years, hydrogel dressings based on the theory of moist wound healing have become a research hotspot in wound care due to their high water content and biomimetic structural characteristics. Further integration with silk fibroin, a natural biomaterial, can significantly optimize the biological function and wound adaptability of hydrogels.
[0003] Silk fibroin, derived from natural silk, possesses a unique β-sheet crystal structure and a hydrophilic amino acid composition (such as glycine and alanine), giving hydrogel dressings dual advantages: 1) dynamic regulation of the moist environment; 2) enhanced bioactivity and tissue compatibility. By combining silk fibroin with hydrogel technology, this new generation of dressings not only overcomes the dryness and adhesion defects of traditional dressings, but also provides an innovative solution for complex wound healing that combines biomimetic structure and proactive function through the integration of natural active ingredients and precise regulation of the microenvironment.
[0004] Chinese invention patent (CN201710088689.1) discloses an invisible and peelable silk fibroin care film base and its preparation method. This base uses silk fibroin as the main component, supplemented with moisturizers and thickeners. The raw materials are all-natural, the preparation method and steps are simple, and when evenly applied to the skin, it can form an aesthetically pleasing invisible film in a short time. The film material is similar to skin, easy to peel off, and has good compatibility with active substances. However, this base has significant limitations when used on wounds. When applied to slightly larger wounds, it cannot form a complete film, requiring multiple peelings to completely remove it. Furthermore, when the wound is pulled, the formed film tends to bulge locally, which is unsightly and does not provide adequate wound coverage. The amount of base applied is difficult to control; a thin application does not easily form a complete film and tends to pill, while a thick application results in the base accumulating on the skin surface, failing to form a film or be absorbed, making cleaning difficult and potentially causing secondary damage to the wound during washing.
[0005] Chinese invention patent (CN201910984317.6) discloses a skin repair dressing and a method for preparing the skin repair dressing. The dressing mainly consists of silk fibroin and / or silk peptides, hyaluronic acid, glycerin, preservatives, and water, and can form a protective barrier on the skin surface, promoting wound healing. However, the addition of preservatives to this dressing may cause some irritation to damaged skin or wounds.
[0006] Chinese invention patent (CN202210758330.1) discloses a silk fibroin hydrogel dressing, its preparation method, and its application. The dressing is composed of 0.01%–15% silk fibroin and 85%–99.9% water. This dressing has a single composition and limited effectiveness in repairing skin damage. Summary of the Invention
[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a silk fibroin gel dressing, its preparation method, and its applications. This silk fibroin gel dressing is easy to apply and absorb, requiring no rinsing; compared to traditional dressings, it uses safer ingredients, is gentle, painless, and non-irritating; the synergistic effect of multiple components forms a multi-dimensional protective layer on the skin surface, repairing skin barrier damage, achieving moisturizing, soothing, redness-reducing, and stabilizing effects, and can also alleviate postoperative skin discomfort and promote wound healing. To achieve the above objectives, the technical solution adopted by this invention is as follows:
[0008] In a first aspect, a silk fibroin gel dressing includes a silk fibroin gel dressing main formulation, wherein the silk fibroin gel dressing main formulation includes water, silk fibroin, an inducer, a protectant, and a thickener.
[0009] The silk fibroin has a mass fraction of 0.1% to 5%, the inducing agent has a mass fraction of 1% to 40%, the protective agent has a mass fraction of 1% to 10%, the thickener has a mass fraction of 0.1% to 2%, and the remainder is water.
[0010] In some of these embodiments, the inducing agent includes one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerol, butylene glycol, propylene glycol, pentanediol, and hexanediol.
[0011] In some of these embodiments, the protective agent includes one or more of trehalose, glycerol, and poloxamer.
[0012] In some of these embodiments, the thickener includes one or more of carbomer, carrageenan, sodium alginate, xanthan gum, and hydroxyethyl cellulose.
[0013] In some embodiments, where the thickener is carbomer, the silk fibroin-gel dressing formulation further includes triethanolamine and / or sodium hydroxide.
[0014] In some embodiments, a silk fibroin gel dressing adjuvant formulation is also included, the silk fibroin gel dressing adjuvant formulation comprising one or more of dextran, hyaluronic acid, collagen, and chitosan;
[0015] The components include 0.01%-10% dextran, 0.1%-1.5% hyaluronic acid, 0.01%-2% collagen, and 0.1%-3% chitosan.
[0016] Secondly, the present invention also provides a method for preparing a silk fibroin gel dressing, comprising:
[0017] Add 0.1% to 2% by mass of thickener to water and stir until homogeneous to obtain the first mixed solution;
[0018] An inducing agent with a mass fraction of 1% to 40% and a protective agent with a mass fraction of 1% to 10% are added sequentially to the first mixed solution and stirred until homogeneous to obtain a second mixed solution.
[0019] Add 0.1% to 5% silk fibroin by mass to the second mixed solution and stir until homogeneous to obtain the third mixed solution;
[0020] The third mixed solution is subjected to moist heat sterilization or irradiation sterilization to obtain silk fibroin gel dressing.
[0021] In some embodiments, the stirring speed for adding 0.1% to 2% by mass of thickener to water and stirring to obtain a first mixed solution is 150 to 250 rpm; and / or
[0022] The inducing agent (1%–40% by mass) and the protective agent (1%–10% by mass) are sequentially added to the first mixed solution and stirred until homogeneous to obtain the second mixed solution. The stirring temperature is 40°C–50°C and the stirring speed is 100–200 rpm; and / or
[0023] The process of adding 0.1% to 5% silk fibroin to the second mixed solution and stirring until a third mixed solution is obtained is carried out at a stirring temperature of 50°C to 60°C and a stirring speed of 250 to 350 rpm.
[0024] In some embodiments, the step of adding 0.1% to 2% by mass of thickener to water and stirring until homogeneous to obtain a first mixed solution further includes:
[0025] After adding 0.1% to 2% by mass of thickener to water, one or more of dextran, hyaluronic acid, collagen, and chitosan are added and stirred until homogeneous to obtain the first mixed solution.
[0026] Thirdly, the present invention also provides the application of a silk fibroin gel dressing based on the first aspect in the fields of medical aesthetics, medical devices and cosmetics, including the care and repair of chronic and non-chronic wounds, as well as skin moisturizing, soothing and anti-aging.
[0027] The present invention adopts the above technical solution and has the following technical effects compared with the prior art:
[0028] This invention discloses a silk fibroin gel dressing, its preparation method, and its application. It is easy to apply and absorb, and does not require rinsing. Compared with traditional dressings, it uses safer ingredients, is gentle, painless, and non-irritating. Multiple ingredients work synergistically to form multidimensional protection on the skin surface, repair skin barrier damage, achieve moisturizing, soothing, redness reduction, and stabilization effects, and can also relieve postoperative skin discomfort and promote wound healing. Attached Figure Description
[0029] Figure 1 shows the experimental data results of the cell proliferation experiment in the embodiment of the present invention;
[0030] Figure 2 shows the experimental data results of the ROS (reactive oxygen species) level test in the embodiment of the present invention. Detailed Implementation
[0031] To make the objectives, technical solutions, and advantages of this application clearer, the application is described and illustrated below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application. All other embodiments obtained by those skilled in the art based on the embodiments provided in this application without inventive effort are within the scope of protection of this application.
[0032] Obviously, the accompanying drawings described below are merely some examples or embodiments of this application. Those skilled in the art can apply this application to other similar scenarios based on these drawings without any inventive effort. Furthermore, it is understood that although the efforts made in this development process may be complex and lengthy, for those skilled in the art related to the content disclosed in this application, any changes to design, manufacturing, or production based on the technical content disclosed in this application are merely conventional technical means and should not be construed as insufficient disclosure of the content of this application.
[0033] In this application, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that is mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this application may be combined with other embodiments without conflict.
[0034] Example 1
[0035] This embodiment relates to the silk fibroin gel dressing of the present invention.
[0036] An illustrative embodiment of the present invention provides a silk fibroin gel dressing, comprising a silk fibroin gel dressing main formulation, which includes water, silk fibroin, an inducer, a protectant, and a thickener; wherein the mass fraction of silk fibroin is 0.1% to 5%, the mass fraction of the inducer is 1% to 40%, the mass fraction of the protectant is 1% to 10%, the mass fraction of the thickener is 0.1% to 2%, and the balance is water.
[0037] It should be noted that silk fibroin gel dressings can be in liquid, gel, or paste form.
[0038] In some embodiments, the inducing agent includes one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerin, butylene glycol, propylene glycol, pentanediol, and hexanediol; preferably, the inducing agent includes polyethylene glycol 400.
[0039] In some embodiments, the protective agent includes one or more of trehalose, glycerol, and poloxamer; preferably, the protective agent includes trehalose and glycerol.
[0040] In some embodiments, the thickener includes one or more of carbomer, carrageenan, sodium alginate, xanthan gum, and hydroxyethyl cellulose; preferably, the thickener includes carbomer.
[0041] Furthermore, when the thickener is carbomer, the main formulation of the silk fibroin gel dressing also includes triethanolamine and / or sodium hydroxide; preferably, the main formulation of the silk fibroin gel dressing also includes triethanolamine.
[0042] In this embodiment, the main formulation of the silk fibroin gel dressing includes 0.1% silk fibroin by mass, 0.4% carbomer by mass, 10% trehalose by mass, 10% glycerin by mass, 15% polyethylene glycol 400 by mass, 1% triethanolamine by mass, and the balance being purified water.
[0043] The advantages of this embodiment are that it is easy to apply and absorb, and does not require rinsing; compared with traditional dressings, it uses safer ingredients, is gentle, painless and non-irritating; multiple ingredients work synergistically to form multidimensional protection on the skin surface, repair skin barrier damage, achieve moisturizing and soothing, reduce redness and stabilize the skin, and relieve postoperative skin discomfort and promote wound healing.
[0044] Example 2
[0045] This embodiment is a modified embodiment of embodiment 1.
[0046] The main formulation of the silk fibroin gel dressing includes 0.1% silk fibroin, 0.8% carbomer, 5% trehalose, 10% glycerin, 15% polyethylene glycol 400, 1% triethanolamine, and the remainder is purified water.
[0047] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0048] Example 3
[0049] This embodiment is a modified embodiment of Embodiments 1 and 2.
[0050] The main formulation of the silk fibroin gel dressing includes 3% silk fibroin, 0.4% carbomer, 10% trehalose, 10% glycerin, 15% polyethylene glycol 400, 1% triethanolamine, and the balance being purified water.
[0051] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0052] Example 4
[0053] This embodiment is a modified embodiment of embodiments 1 to 3.
[0054] The main formulation of the silk fibroin gel dressing includes 3% silk fibroin, 0.8% carbomer, 5% trehalose, 10% glycerin, 15% polyethylene glycol 400, 1% triethanolamine, and the balance being purified water.
[0055] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0056] Example 5
[0057] This embodiment is a modified embodiment of embodiments 1 to 4.
[0058] The main formulation of the silk fibroin gel dressing includes 5% silk fibroin, 0.4% carbomer, 10% trehalose, 10% glycerin, 15% polyethylene glycol 400, 1% triethanolamine, and the balance being purified water.
[0059] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0060] Example 6
[0061] This embodiment is a modified embodiment of embodiments 1 to 5.
[0062] The main formulation of the silk fibroin gel dressing includes 5% silk fibroin, 0.8% carbomer, 5% trehalose, 10% glycerin, 15% polyethylene glycol 400, 1% triethanolamine, and the balance being purified water.
[0063] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0064] Example 7
[0065] This embodiment is a modified embodiment of embodiments 1 to 6.
[0066] The silk fibroin gel dressing also includes auxiliary formulations. These auxiliary formulations include one or more of the following: dextran, hyaluronic acid, collagen, and chitosan; wherein the mass fraction of dextran is 0.01%–10%, the mass fraction of hyaluronic acid is 0.1%–1.5%, the mass fraction of collagen is 0.01%–2%, and the mass fraction of chitosan is 0.1%–3%.
[0067] Preferably, the mass fraction of dextran is 0.1%, the mass fraction of hyaluronic acid is 0.1%, the mass fraction of collagen is 0.05%, and the mass fraction of chitosan is 1%.
[0068] The advantages of this embodiment are the same as those of Embodiment 1, and will not be repeated here.
[0069] Example 8
[0070] The physicochemical properties of the silk fibroin gel dressings prepared in Examples 1-6 were tested, including appearance, pH, and viscosity. The appearance of the silk fibroin gel dressings was observed visually. The pH was measured using a pH meter, and the viscosity was measured using a rotational viscometer. The test results are as follows:
[0071] Carbomer and triethanolamine work synergistically to form a stable polymer structure with excellent adhesive properties. A comparison of Examples 3 and 4 shows that the higher the carbomer content, the more viscous the gel dressing becomes.
[0072] A comparison of Examples 5 and 6 shows that the viscosity is significantly increased after carbomer is combined with silk fibroin.
[0073] Therefore, by adjusting the ratio of these three components, an ideal gel dressing product can be obtained. This silk fibroin gel dressing can form multi-dimensional protection on the skin surface, ensuring that damaged skin wounds are effectively isolated from the external environment, thus achieving a protective effect.
[0074] The physicochemical properties of each embodiment in the table above did not change significantly before and after moist heat sterilization, indicating that the silk fibroin gel dressing prepared under this formula and process is relatively stable and the silk fibroin forms a stable β-sheet structure.
[0075] Example 9
[0076] Sensory evaluation was performed on the silk fibroin gel dressings prepared in Examples 1-6. The dressings were applied to the skin on the inside of the wrist, and the gel texture, spreadability, skin feel, and irritation were observed.
[0077] As can be seen from Examples 4 and 6, when the silk fibroin gel dressing has a high viscosity, it is not easy to spread and the skin feels sticky, resulting in a poor user experience.
[0078] As can be seen from Examples 1, 2, 3 and 5, a silk fibroin gel dressing with appropriate viscosity has a uniform texture, is smooth to apply, and leaves the skin feeling cool and moisturized after application. It is gentle and non-irritating and can relieve postoperative skin discomfort.
[0079] Example 10
[0080] Skin moisture content tests were conducted on the silk fibroin gel dressings prepared in Examples 1-6. The dressings were applied to the cheeks and massaged in after 10 minutes. Skin moisture, elasticity, and oil content were measured before and after application using a skin moisture meter. The entire test was conducted in a constant temperature and humidity environment. The results are as follows:
[0081] As can be seen from the table above, the silk fibroin gel dressing provided by the present invention has a good moisturizing effect on the skin, and can improve skin elasticity, thereby achieving the effects of moisturizing, soothing, and repairing skin barrier damage.
[0082] Example 11
[0083] The test was conducted according to the method specified in YY / T 0471.1-2004 "Test Methods for Contact Wound Dressings" Part 1: Liquid Absorbency 3.4 Liquid Affinity of Amorphous Hydrogel Dressings. The ability of silk fibroin gel dressings to absorb liquid from agar and deliver liquid to gelatin was tested to evaluate the liquid affinity of the gel dressing.
[0084] As can be seen from Examples 1 and 2, when the silk fibroin content is low, the silk fibroin gel dressing has limited ability to absorb wound exudate.
[0085] As can be seen from Example 6, when the silk fibroin content is high, the skin's ability to provide moisture is weakened.
[0086] As can be seen from Examples 3 and 5, the addition of trehalose works synergistically with silk fibroin. While absorbing wound exudate, it can also provide a certain amount of moisture to the skin, creating a physiologically moist healing environment for wound healing and promoting wound healing.
[0087] Example 12
[0088] Cell proliferation experiments were conducted on the silk fibroin gel dressings prepared in Examples 1-6, and the specific methods are as follows:
[0089] Fibroblasts were seeded into 96-well plates and cultured in DMEM medium containing 10% FBS at 37°C, 5% CO2, and saturated humidity. After 24 hours of culture, silk fibroin gel dressing samples were placed in the culture medium and incubated for 72 hours. CCK-8 (cell counting kit-8) was then added, and the absorbance at 450 nm was measured using a microplate reader.
[0090] As shown in Figure 1, after co-culturing with silk fibroin gel for 72 hours, the fibroblasts in the groups of Examples 1-6 showed significant growth compared to the control group, indicating that the silk fibroin gel dressing can promote the growth and proliferation of fibroblasts.
[0091] Example 13
[0092] The ROS (reactive oxygen species) levels of the silk fibroin gel dressings prepared in Examples 1, 3, and 5 were tested using the following methods:
[0093] Fibroblasts were seeded and cultured in 12-well plates in DMEM medium containing 10% FBS at 37°C, 5% CO2, and saturated humidity. After 48 hours of culture, silk fibroin gel dressing samples were placed in the culture medium and incubated for another 48 hours. DCFH-DA fluorescence was then added, followed by UVA irradiation. Immediately after irradiation, the fluorescence intensity was detected using a fluorescence microplate reader.
[0094] Experimental groups: The negative group consisted of unirradiated fibroblasts; the positive group consisted of irradiated fibroblasts; the control group consisted of fibroblasts supplemented with 0.5 mg / mL vitamin C; and the experimental group consisted of silk fibroin gel dressings from Examples 1, 3, and 5 supplemented with fibroblasts.
[0095] As shown in Figure 2, compared with the positive group, the ROS (reactive oxygen species) levels in the experimental group decreased to varying degrees, indicating that the silk fibroin gel dressing can reduce the level of reactive oxygen species (ROS).
[0096] Example 14
[0097] This embodiment relates to a method for preparing the silk fibroin gel dressing of the present invention.
[0098] A method for preparing a silk fibroin gel dressing, comprising:
[0099] Add 0.1% to 2% by mass of thickener to water and stir until homogeneous to obtain the first mixed solution;
[0100] An inducing agent with a mass fraction of 1% to 40% and a protective agent with a mass fraction of 1% to 10% are added sequentially to the first mixed solution and stirred until homogeneous to obtain a second mixed solution.
[0101] Add 0.1% to 5% silk fibroin by mass to the second mixed solution and stir until homogeneous to obtain the third mixed solution;
[0102] The third mixed solution was subjected to moist heat sterilization to obtain silk fibroin gel dressing.
[0103] In some of these embodiments, the thickener includes, but is not limited to, carbomer.
[0104] It should be noted that when the thickener is carbomer, triethanolamine also needs to be added.
[0105] In some of these embodiments, the inducing agent includes, but is not limited to, polyethylene glycol 400.
[0106] In some of these embodiments, the protective agent includes, but is not limited to, trehalose and glycerin.
[0107] It should be noted that the stirring speed for adding 0.1% to 2% of the thickener by mass to water and stirring until a first mixed solution is obtained is 150 to 250 rpm; preferably, the stirring speed is 200 rpm.
[0108] It should be noted that the inducing agent with a mass fraction of 1% to 40% and the protective agent with a mass fraction of 1% to 10% are added to the first mixed solution in sequence and stirred evenly to obtain the second mixed solution. The stirring temperature is 40℃ to 50℃ and the stirring speed is 100 to 200 rpm; preferably, the stirring temperature is 45℃ and the stirring speed is 150 rpm.
[0109] It should be noted that the stirring temperature for adding 0.1% to 5% silk fibroin to the second mixed solution and stirring until uniform to obtain the third mixed solution is 50°C to 60°C and the stirring speed is 250 to 350 rpm; preferably, the stirring temperature is 55°C and the stirring speed is 300 rpm.
[0110] Further, adding a thickener with a mass fraction of 0.1% to 2% to water and stirring until homogeneous to obtain a first mixed solution also includes adding one or more of dextran, hyaluronic acid, collagen, and chitosan to water after adding a thickener with a mass fraction of 0.1% to 2% to water and stirring until homogeneous to obtain a first mixed solution.
[0111] Example 15
[0112] This embodiment relates to the application of the silk fibroin gel dressing of the present invention in the fields of medical aesthetics, medical devices and cosmetics.
[0113] Silk fibroin gel dressings can be used in medical aesthetics, medical devices and cosmetics to care for and repair chronic and non-chronic wounds, as well as moisturize, soothe and anti-age the skin.
[0114] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0115] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.
Claims
1. A silk fibroin gel dressing, characterized by, The silk fibroin gel dressing main formula comprises water, silk fibroin, an inducing agent, a protective agent, a thickening agent, and the like. The mass fraction of the silk fibroin is 0.1% to 5%, the mass fraction of the inducing agent is 1% to 40%, the mass fraction of the protective agent is 1% to 10%, the mass fraction of the thickening agent is 0.1% to 2%, and the balance is water.
2. The silk fibroin gel dressing according to claim 1, wherein, The inducing agent comprises one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerol, butanediol, propylene glycol, pentanediol, and hexanediol.
3. The silk fibroin gel dressing according to claim 1, wherein, The protective agent comprises one or more of trehalose, glycerol, and poloxamer.
4. The silk fibroin gel dressing according to claim 1, wherein, The thickening agent comprises one or more of carbomer, carrageenan, sodium alginate, xanthan gum, and hydroxyethyl cellulose.
5. The silk fibroin gel dressing according to claim 4, wherein, In the case where the thickening agent is carbomer, the silk fibroin gel dressing main formula further comprises triethanolamine and / or sodium hydroxide.
6. The silk fibroin gel dressing of claim 1, wherein, The silk fibroin gel dressing auxiliary formula comprises one or more of dextran, hyaluronic acid, collagen, and chitosan. The mass fraction of the dextran is 0.01% to 10%, the mass fraction of the hyaluronic acid is 0.1% to 1.5%, the mass fraction of the collagen is 0.01% to 2%, and the mass fraction of the chitosan is 0.1% to 3%.
7. A method of preparing a silk fibroin gel dressing, characterized by, The method comprises the following steps: A thickening agent with a mass fraction of 0.1% to 2% is added to water and stirred uniformly to obtain a first mixed solution; An inducing agent with a mass fraction of 1% to 40% and a protective agent with a mass fraction of 1% to 10% are sequentially added to the first mixed solution and stirred uniformly to obtain a second mixed solution; Silk fibroin with a mass fraction of 0.1% to 5% is added to the second mixed solution and stirred uniformly to obtain a third mixed solution; The third mixed solution is subjected to moist heat sterilization or irradiation sterilization to obtain a silk fibroin gel dressing.
8. The preparation method according to claim 7, characterized in that, The stirring speed for adding the thickening agent with a mass fraction of 0.1% to 2% to water and stirring uniformly to obtain the first mixed solution is 150 to 250 rpm; and / or The stirring temperature and speed for sequentially adding the inducing agent with a mass fraction of 1% to 40% and the protective agent with a mass fraction of 1% to 10% to the first mixed solution and stirring uniformly to obtain the second mixed solution are 40°C to 50°C and 100 to 200 rpm, respectively; and / or The stirring temperature and speed for adding the silk fibroin with a mass fraction of 0.1% to 5% to the second mixed solution and stirring uniformly to obtain the third mixed solution are 50°C to 60°C and 250 to 350 rpm, respectively.
9. The preparation method according to claim 7, characterized in that, The step of adding the thickening agent with a mass fraction of 0.1% to 2% to water and stirring uniformly to obtain the first mixed solution further comprises the following steps: After the thickening agent with a mass fraction of 0.1% to 2% is added to water, one or more of dextran, hyaluronic acid, collagen, and chitosan are added thereto and stirred uniformly to obtain the first mixed solution.
10. Use of a silk fibroin gel dressing according to any one of claims 1 to 6 in the field of medical aesthetics, medical devices and cosmetics, characterized in that, The method is used for the care, repair of chronic and non-chronic wounds, and skin moisturizing, soothing, and anti-aging.