The invention relates to the technical field of medical plastic surgery, in particular to the technical field of facial filling and anti-wrinkle, in particular, it is an injection filling cosmetic modification anti-aging, skin beautification and facial rejuvenation material. The preparation formula of the present invention includes hyaluronic acid, collagen, vitamin E and PLGA. The preparation method comprises: 1) preparing high-viscosity gel; 2) preparing PLGA nano-microspheres; 3) fully mixing high-viscosity gel and PLGA nano-microspheres; 4) sterilizing and filling. Compared with the prior art, the present invention has the beneficial effect that: the combined use of collagen and hyaluronic acid reduces skin erythema, swelling, hardening or itching, muscle pain, urticaria or Other symptoms such as rash. It reduces the probability of severe complications such as peripheral blood circulation embolism, skin necrosis, retinal artery embolism, and cardiocerebral artery embolism that may be caused by simple hyaluronic acid injection, and the safety of product use is improved.