46 results about "Enterobacter aerogene" patented technology

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter aerogenes and belongs to the technical field of biological detection. The enterobacter aerogenes specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-GTAACCGGTGAAACCGAAAGC-3'; and the reverse primer R is 5'-GATGCCGCCTTCGTAGTGGAAATGG-3'. A detection method for detecting the enterobacter aerogenes, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The invention belongs to the technical field of medicines, relates to an antibiotic combination containing Avibactam and an application of the antibiotic combination, provides an application of Avibactam or pharmaceutically acceptable salt and carbapenems antibiotics to preparation of medicines for treating or preventing bacterial infection, particularly an application of the Avibactam to preparation of medicines for treating infection caused by escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, baumanii and enterobacter aerogenes so as to solve the problem of drug resistance of the carbapenems antibiotics.

A compound method of high-yield nucleoside phosphorylase strains and arabinose nucleoside pertains to biochemical engineering field, in particular to the high-yield nucleoside phosphorylase strains and a method for compounding arabinose purine nucleoside with the strains by an enzyme method. The invention aims at solving a technical problem for providing the strains of the high-yield nucleoside phosphorylase and strains of uridine phosphorylase and the method for producing the arabinose purine nucleoside with the strains. The invention discloses enterobacter aerogenes with a preservation number of CGMCC No.2035 and the method for producing the arabinose purine nucleoside with the strains, and the invention comprises steps that (1) the enterobacter aerogenes DWOQ-58 of the invention is cultured and collected, and (2) the enterobacter aerogenes DWOQ-58 is contacted with arabinose donor and receptors of purine base. The strains of the invention are rich in vigor and resists 5-flucytosine with an average conversion rate of more than 80 percent in general and the reaction time of the invention is shortened to less than 12 hours.

The invention provides a specific oligonucleotide sequence for detecting serotypes 1-8 of enterobacter aerogenes. The oligonucleotide contains: (1), a DNA fragment selected from wzy genes of the enterobacter aerogenes of types 1, 2, 3, 5, 6, 7 and 8; (2), a DNA fragment selected from orf8 genes of the enterobacter aerogenes of type 4. A suspension array detection system and method for the serotypes 1-8 of the enterobacter aerogenes are established through combination with a Bio-Plex 200 suspension array system of the Bio-Rad company, the blank of serological typing technology of the enterobacter aerogenes is filled up, and the invention has great significance in clinical detection and epidemiological monitoring of the important pathogenic bacterium.

The invention provides a gene chip for detecting common pathogenic bacteria in cosmetics. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises DNA segments which are selected from 16S-23SrDNA intermediate zones of micrococcus luteus, staphylococcus aureus, streptococcus pyogenes, salmonella, proteus mirabilis, citrobacter freundii, pseudomonas aeruginosa, enterobacter aerogenes and providencia stuartii and 18S-28SrDNA intermediate zones of the ipaH gene of shigella, candida albicans and cryptococcus neoformans, or complementary DNA or RNA sequences of the DNA segments. The invention also provides a kit for detecting the common pathogenic bacteria in the cosmetics by use of the gene chip. The gene chip and the kit can be used for detecting the common pathogenic bacteria in the cosmetics, and are simple and convenient to operate, high in accuracy and excellent in repeatability.

The invention provides a kit for the detection of Enterobacter cloacae, including guide RNAs specifically targeting the rpoB gene of the Enterobacter cloacae, a primer pair for amplification, hydrated TwistAmp basic kit reaction drying balls, LbCas12a proteins, a single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer. The kit can be used to detect the Enterobacter cloacae with high specificity, and can distinguish the Enterobacter cloacae from other Enterobacter bacteria, including Enterobacter aerogenes, Enterobacter gergoviae and Enterobacter sakazakii. At the same time, the method using the RNA sequence described in the invention for detection takes about 1 hour, does not need any PCR instruments, and has low requirements for equipment; and time consumption of the method is significantly lower than that of the conventional bacterial culture method or the PCR sequencing method, with great clinical practical value.

The invention discloses an Enterobacter aerogenes S27 for degrading malachite green. The Enterobacter aerogenes with the preservation number CCTCC NO: M 2017452 is preserved on Aug. 28, 2017 in ChinaCenter For Type Culture Collection in Wuhan University in Wuhan province of China. The Enterobacter aerogenes resists salt, is able to adapt to harsh environment, is low-cost in use, can recover ecological balance, and is applicable to degradation of the malachite green in wastewater, thereby serving as a beneficial strain resource for environment pollution caused by the malachite green.

The invention provides a reagent kit for detecting enterobacter aerogenes. The reagent kit contains a guide RNA specifically targeted to an enterobacter aerogenes rpoB gene, an amplification primer pair, a hydration TwistAmp basic kit reaction drying ball, LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease inhibitor and a buffer solution. When the reagent kit is used for detecting the enterobacter aerogenes, the detection specificity is high, and the enterobacter aerogenes can be distinguished from other enterobacter bacteria including enterobacter cloacae, enterobacter gergoviae and enterobacter sakazakii. Besides, when the RNA sequence is used for detection, time consumption is about 1 hour, a PCR instrument is not needed, requirements for equipment are low and are notably lower than those by a traditional bacteria culture method or a PCR sequencing method, and the clinical use value is high.

The invention discloses a spiro quinazoline derivative and a preparation method thereof. The molecular structural formula of the spiro quinazoline derivative is as shown in the specification, wherein R1 is any one or two out of hydrogen, methyl, trifluoromethyl, fluorine or chlorine, and R2 is hydrogen, methyl or phenyl. The spiro quinazoline derivative has an excellent antibacterial effect particularly on pseudomonas aeruginosa, enterobacter aerogenes and staphylococcus epidermidis, and has the better antibacterial effect on the pseudomonas aeruginosa, the enterobacter aerogenes and the staphylococcus epidermidis than a marketed bactericide bromogeramine, and the preparation method has the advantages of simple process, mild condition, high productivity, simple and convenient post treatment, high activity of an obtained product and the like.

The invention belongs to the field of quality control in microbial detection and particularly relates to a standard sample of salmonella in milk powder and a preparation method of the standard sample of the salmonella in the milk powder. The standard sample of the salmonella in the milk powder comprises a target bacterium and background flora, wherein the target bacterium is the salmonella; the background flora comprises Bacillus cereus, Staphylococcus aureus, Enterobacter cloacae and Enterobacter aerogenes. The standard sample meets requirements for uniformity and stability, the stability of the standard sample can be guaranteed to the greatest extent, and a perfect preparation technology for the standard sample is formed by starting from meeting special transport conditions by means of research in special process, stability and uniformity and the like, is suitable for preparing the standard sample, meeting the requirements for uniformity and stability, of the salmonella in the milk powder and is applicable to quality control, method validation and other purposes in a laboratory.

The invention discloses a method capable of reducing biogenic amine in a fermented sausage and application thereof. The method comprises the following steps that 1, according to sequences of amine production-correlated genes luxR of main amine production bacteria enterobacter aerogenes and enterobacter cloacae in the fermented sausage, small-interfering ribonucleic acid (siRNA) is designed and prepared; 2, the siRNA is used as an encapsulated matter for preparing lipidosome, so that the stable siRNA cationic liposome is obtained; 3, the siRNA cationic liposome is added into the fermented sausage according to a certain ratio in a fermentation early stage. According to the method, the flavor, color and lustre of the fermented sausage are not affected, and the effect of inhibiting the biogenic amine is significant, so that the method has important significance in controlling the biogenic amine in the fermented sausage.

The invention provides a microbial agent combining enterobacter aerogenes and klebsiella aerogenes, the microbial agent is used for treating lead and cadmium polluted soil, and the microbial agent comprises enterobacter aerogenes and klebsiella aerogenes; the enterobacter aerogenes is Enterobacter aerogenes W6, the Latin classification name of the enterobacter aerogenes is Enterobacter aerogenes, the enterobacter aerogenes is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No.22888. The enterobacter aerogenes can be used for preparing the feed additive, and the feed additive can be used for preparing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive. The Klebsiella aerogenes are Klebsiella aerogenes Wn, the classification name of the Klebsiella aerogenes is Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is the Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No. 22889.

The invention belongs to the technical field of medicines. The invention relates to an antibacterial composition containing avibactam and meropenem and application thereof. Particularly, the inventionprovides application of avibactam or a pharmaceutically acceptable salt thereof and meropenem in preparation of a medicine for treating or preventing bacterial infection, in particular to applicationin preparation of medicines for treating infection caused by escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and enterobacter aerogenes, and aims to solve theproblem of drug resistance of carbapenem antibiotics.

The invention discloses a hydrogen production associated protein, coding genes thereof and application thereof. The hydrogen production associated protein is a protein (a) or protein (b), wherein the protein (a) has an amino acid sequence represented by the No.1 sequence 1 in a sequence table; or (b) the protein has an amino acid sequence that has substituents of one or more amino acid residues, lacks one or more amino acid residues or has one or more additional amino acid residues and is associated with the hydrogen production and derived from the sequence 1. The invention also obtains a strain of engineering bacteria by inactivating the coding genes of the protein in the enterobacter aerogenes, and the engineering bacteria have the advantages of high growing speed and strong environmental adaptability, greatly improve the glucose utilizing ability and the hydrogen producing ability, can be applied to biological hydrogen production, and have great instruction significance.

The invention provides a stabilizer of enterobacter aerogenes. The stabilizer comprises the following raw materials: L-carnosine, polyethylene glycol, skim milk powder, glycine and ascorbic acid. Thestabilizer of the enterobacter aerogenes quality control bacteria can make the freeze-drying survival rate of the quality control bacteria reach 95% or above, and also can keep the bacterial content stability in the long-term storage process.

The invention discloses a method for regenerating oxidized coenzyme I by utilizing the transformation of intact cell organisms. The method for regenerating the oxidized coenzyme I comprises the following steps: in a buffer solution, reduced coenzyme I is taken as a substrate and transformed into the oxidized coenzyme I by utilizing Enterobacter aerogenes. The pH value of the buffer solution is between 4.0 and 9.0. The concentration of the reduced coenzyme I in the buffer solution is between 0.02 and 10mM. The method for regenerating the oxidized coenzyme I can transform NADH into NAD<+>, and the transformation rate reaches 72 percent.

The invention discloses a LAMP primer group for detecting enterobacter aerogenes, a kit and a rapid detection method. The LAMP primers are six specific primers designed according to a ThdF gene of enterobacter aerogenes. By applying the six primers, specific amplification can be realized, the enterobacter aerogenes can be effectively identified, and the lowest detection limit can reach 1 DNA copy; a DNA sample can be detected within 60min; the detection method can be flexibly selected according to actual conditions, and the primer group is convenient and rapid and has a very wide application prospect.