24 results about "Protein recombinants" patented technology

The invention relates to the technical field of immunotherapy, in particular to a regulatory protein recombinant vaccine and application thereof in pulmonary fibrosis treatment. The vaccine contains polypeptide of amphiregulin antigen epitopes, and the amino acid sequence of the vaccine is shown as SEQ ID No. 1. A research result shows that the recombinant vaccine can effectively induce generation of a specific antibody aiming at the AREG protein in a mouse body, so that secretion of the AREG protein and activation of downstream receptors of the AREG protein in the pulmonary fibrosis process are inhibited. By reducing collagen deposition in lung tissue and reducing tissue structure damage, the recombinant vaccine significantly reduces gene expression of fibrosis-related protein, recovers lung functions of pulmonary fibrosis model mice, and inhibits further development of diseases. In addition, the recombinant vaccine can be administered in an aerosol inhalation mode, and has broad-spectrum, efficient and long-term stable expression and good safety. The invention provides a brand-new targeted therapy means for clinical prevention and treatment of pulmonary fibrosis, and shows a wide application prospect.

The invention provides an extracellular matrix protein recombinant protein, a nucleic acid molecule, a recombinant vector, a genetically engineered bacterium, a preparation method and an application. The provided extracellular matrix protein recombinant protein comprises the following protein fragments: human fibronectin, humanized collagen and elastin-like protein. Wherein the amino acid sequence of the human fibronectin is as shown in SEQ ID NO. 1; the amino acid sequence of the humanized collagen is as shown in SEQ ID NO. 2; the elastin-like protein comprises a pentapeptide repetitive sequence, repetitive units of the pentapeptide repetitive sequence comprise-[valine-proline-glycine-Xaa-glycine] n-, Xaa is isoleucine or lysine, and the number n of the repetitive units is 15-30; in the extracellular matrix protein recombinant protein, any two adjacent ones of the human fibronectin, the humanized collagen and the elastin-like protein are independently connected through a peptide bond or a connecting peptide. The extracellular matrix recombinant fusion protein provided by the invention has good thermal stability and can promote cell adhesion.

The invention relates to a preparation method of high-protein recombinant rice capable of being rehydrated quickly. The main component of the recombinant rice is broken rice, and auxiliary materials comprise high-amylose corn starch, wheat protein, pea protein, guar gum and monoglyceride which are uniformly mixed in proportion. Wherein the addition amount of the high amylose corn starch is 0.5-5%, the addition amount of the wheat protein and the addition amount of the pea protein are both 5-15%, and the addition amount of the guar gum and the addition amount of the monoglyceride are both 0.05-0.2%. The recombinant rice obtained by the preparation method is short in rehydration time, proper in taste, high in protein content and strong in satiety, has the characteristic of slow digestion, effectively solves the problems of overlong rehydration time, half-cooked taste, poor taste and the like of the existing recombinant rice product in the cooking process, and meets the requirements of high-protein diet and low digestion rate of specific people.

The application discloses application of a micro cryptosporidium cgd6_660 protein as an oocyst outer wall marker protein, and discloses that the inventors find that the cryptosporidium cgd6_660 protein is an oocyst outer wall protein, and the protein is located on the outer surface of the cryptosporidium oocyst wall. The inventors confirm the feasibility of the protein as a detection antigen. The antibody of the protein recombinant protein (as shown in a sequence table SEQ ID N0.2) can be used for immunological detection of unbroken cryptosporidium oocysts (live cryptosporidium oocysts), and the application further provides a specific polypeptide of the protein.

The present invention relates to the field of molecular biology technology, and specifically to an application of a molecular chaperone TF in promoting the soluble expression of type A Seneca virus structural protein VP2. The present invention first constructs a SVA VP2 protein recombinant expression plasmid pET28a-SVA-VP2 based on the gene sequence of the SVA CH / FJ / 2017 strain; then the pET28a-SVA-VP2 recombinant plasmid and the molecular chaperone pTf16 plasmid are co-transfected into BL21 (DE3) competent cells to successfully prepare a soluble recombinant SVA VP2 protein; the recombinant VP2 protein has good immunogenicity in both in vivo and in vitro tests, and can induce the body to produce a strong humoral immune response and cellular immune response, providing experimental materials and theoretical basis for the development of SVA subunit vaccines and diagnostic preparations.

The invention belongs to the technical field of biology, and discloses a porcine circovirus type 3 Cap protein recombinant protein and a preparation method, a kit and application thereof, the PCV3 Cap protein recombinant protein is obtained through truncation design of the PCV3 Cap protein, the coincidence rate of the kit prepared from the recombinant protein and commercially available products exceeds 95%, and the kit has good sensitivity and specificity.

The invention discloses an anti-muscovy duck parvovirus VP3 protein recombinant nano antibody, an expression engineering strain and application thereof, and belongs to the field of biology. The anti-Muscovy duck parvovirus VP3 protein recombinant nano antibody is obtained by screening through technologies of computational biology, molecular docking simulation, deep learning and the like. The method comprises the following steps: firstly, screening a heavy-chain antibody variable region sequence VP3-Nb66, then constructing a recombinant eukaryotic expression plasmid by adopting the heavy-chain antibody variable region sequence VP3-Nb66, and further constructing a recombinant expression engineering strain by adopting the recombinant eukaryotic expression plasmid. And further preparing the Muscovy duck parvovirus VP3 protein recombinant nano antibody by adopting the recombinant expression engineering strain. The yield of the recombinant nano antibody reaches 1.01 mg / L, the ELISA result OD450 is equal to 1.66, and the recombinant nano antibody has remarkable binding activity and can be used for preparing a nano antibody preparation for detecting the muscovy duck parvovirus disease.

The invention provides a porcine circovirus type 3 Cap recombinant protein gene, a recombinant rod grain, a protective antigen and a purification method, and belongs to the technical field of biology. The invention utilizes an insect baculovirus eukaryotic expression system to prepare the porcine circovirus type 3 Cap protein protective antigen, and provides a chromatographic purification process of the porcine circovirus type 3 Cap protein protective antigen, which adopts a one-step column chromatography purification method and adopts splitting, filtering and composite affinity chromatography processes to obtain the porcine circovirus type 3 Cap protein protective antigen. According to the method, the porcine circovirus type 3 Cap protein protective antigen is specifically purified from the complex cell lysis solution rapidly and efficiently, the recovery rate in the purification process is high, the purity reaches 90% or above, the treatment capacity is large, the assembly rate of the purified product is high, and the method is simple and suitable for large-scale production.

The invention discloses a high-heme-yield escherichia coli engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of synthetic biology. According to the engineering bacteria disclosed by the invention, eight genes in a heme C4 synthetic route are designed into two fusion multienzyme complexes, short peptide Linker is adopted for connection, escherichia coli preference codon optimization is performed, and the enzyme synergy efficiency is remarkably improved; and meanwhile, constructing a heme efflux protein recombinant plasmid, and co-transforming the heme efflux protein recombinant plasmid and the fusion multienzyme complex plasmid into an escherichia coli host to form a double-plasmid co-expression system. The engineering bacterium takes glucose / glycerol as a substrate and lactose as an inducer for fermentation, the shake flask yield of the heme reaches up to 865 mg / L and is increased by more than one time compared with the prior art, expensive intermediates such as glutamic acid / glycine do not need to be added, and the bottleneck of intracellular accumulation inhibition is effectively broken through. The fermentation product is directly extracted through the acetic acid solvent, the process is simple, the cost is low, the method is suitable for large-scale production of safe and high-purity heme, and a green biological manufacturing solution is provided for the fields of artificial meat, medicine and health care products.

The invention discloses an N protein recombinant antigen for detecting a new bunyavirus antibody and application of the N protein recombinant antigen. The N protein recombinant antigen comprises an N protein and a peptide fragment containing a linear epitope in the N protein. The N protein recombinant antigen completely covers SFTSV antibody recognition sites and has high activity, and the prepared protein is high in purity, stable in structure and good in batch-to-batch stability.

The invention provides a preparation method of prokaryotic expression staphylococcus aureus arginine repression operon ArgR protein, and belongs to the technical field of genetic engineering. An ArgR protein recombinant expression plasmid pGEX-6P-1-ArgR is constructed on the basis of a pGEX-6P-1 plasmid, a high-expression strain is obtained through expression and screening of escherichia coli BL21, and the soluble staphylococcus aureus arginine repression operon ArgR protein can be obtained after fermentation and purification. The ArgR protein prepared by the invention can be applied to the fields of pharmacy, biomedicine and the like.

The invention discloses an HEK293 cell capable of stably expressing pigeon adenovirus I type Fiber2 protein as well as a construction method and application of the HEK293 cell. A pigeon adenovirus I type Fiber2 protein recombinant expression vector is constructed and introduced into an HEK293 adherent cell to obtain a cell QYH-PAF2-A1 capable of expressing the pigeon adenovirus I type Fiber2 protein, and the cell QYH-PAF2-A1 is domesticated into a suspension cell QYH-PAF2-A1. The cell suspension growth state is good, the passage is stable, and the Fiber2 protein can still be stably expressed after 10 generations of continuous culture; then, the Fiber2 protein expressed by the cell is prepared into a subunit vaccine, the protection rate of the subunit vaccine reaches 80% through an immune challenge test, and pigeon adenovirus I type infection can be effectively prevented and controlled. The suspension cell constructed by the invention can be applied to research and development of pigeon adenovirus I type subunit vaccines, preparation of diagnostic reagents and basic research of virus invasion mechanisms, and has a wide application prospect.

The invention relates to the technical field of molecular biology and immunology, in particular to a bovine herpesvirus type 4 gb protein recombinant expression method and application thereof.The 104-384aa segment (the amino acid sequence is shown as SEQ ID NO.2) of bovine herpesvirus type 4 gb protein is selected and constructed to a pet22b / pet32a escherichia coli expression vector, after induced culture and ultrasonication, Ni-NTA gradient elution purification is conducted, and the recombinant expression vector is obtained. The recombinant gb protein with the molecular weight of about 48.5 kDa is obtained. The recombinant protein is used for preparing mouse antiserum, and the titer of the antiserum is not lower than 1: 128000 through ELISA (enzyme-linked immuno sorbent assay) detection; the purity of the purified antiserum meets experimental requirements, and the antiserum can specifically recognize a target antigen. The method solves the problems of poor protein dissolution and low antiserum titer in the traditional method, is controllable in process and high in cost effectiveness, and provides a reliable tool for molecular detection and immunoassay of the bovine herpesvirus type 4.

The invention provides a method for improving the protein expression quantity of a protein expression system. The method comprises the following steps: replacing a first codon behind an initiation codon in a gene of a target protein with a codon of which the third site is G of the same amino acid; compared with other methods, the method can greatly improve the protein recombinant expression yield and reduce the protein production cost, and is high in cost performance.

The invention discloses the application of Cryptosporidium cgd8_1420 protein as oocyst wall exocyst marker protein, and its amino acid sequence is shown in SEQ ID NO.1 in the sequence table; the invention also discloses Cryptosporidium oocyst wall protein specific polypeptide, and its amino acid sequence is: VLQETIYNFKNFKC; anti-Cryptosporidium oocyst wall exocyst protein antibody, which can specifically bind to the outer surface of the Cryptosporidium oocyst wall exocyst; anti-Cryptosporidium oocyst wall protein specific polypeptide antibody, which is an antibody prepared by adopting the Cryptosporidium oocyst wall protein specific polypeptide; application of anti-Cryptosporidium oocyst wall exocyst protein antibody or anti-Cryptosporidium oocyst wall protein specific polypeptide antibody in detecting Cryptosporidium; oocyst wall exocyst protein, which is located on the outer surface of the Cryptosporidium oocyst wall, and the protein can be used as a detection antigen, and the protein recombinant protein antibody can be used for immunological detection of unbroken Cryptosporidium oocysts.

The invention provides trichogramma japonicum ecdysone regulatory protein recombinant protein as well as preparation and application thereof. The amino acid sequence of the trichogramma japonicum ecdysone regulatory protein recombinant protein is as shown in SEQ ID NO.1, and the nucleotide sequence for coding the recombinant protein is as shown in SEQ ID NO.2. The ecdysone regulatory protein of the trichogramma japonicum is identified for the first time, the recombinant protein with biological activity is prepared, and a preparation method of the recombinant protein comprises the following steps: performing prokaryotic codon optimization on a coding sequence of the ecdysone regulatory protein TjERP of the trichogramma japonicum to obtain a nucleotide sequence, performing gene synthesis on the nucleotide sequence, constructing and expressing plasmids to obtain the recombinant protein. And the recombinant protein is transformed to escherichia coli BL21 (DE3), and is subjected to induced expression and purification to obtain the recombinant protein. The invention also identifies the regulation and control of the ecdysone regulatory protein on the growth and development of trichogramma japonicum. The TjERP recombinant protein provided by the invention can regulate the growth and development and life of adult trichogramma japonicum, and the obtained protein has important application potential in prevention and control of rice pests by utilizing the trichogramma japonicum.

The application belongs to the technical field of biology, discloses a porcine circovirus type 3 Cap protein recombinant protein and a preparation method, a kit and an application thereof, and the PCV3 Cap protein recombinant protein is obtained by truncation design of PCV3 Cap protein. The kit prepared from the recombinant protein has a coincidence rate of more than 95% with a commercially available product and has good sensitivity and specificity.

The application belongs to the technical field of antibody preparation, and particularly relates to a recombinant rabbit monoclonal antibody specifically recognizing type II grass carp reovirus VP56 protein and application thereof. The amino acid sequence of the light chain variable region of the monoclonal antibody is shown in SEQ ID NO. 3, and the amino acid sequence of the heavy chain variable region is shown in SEQ ID NO. 1. The prepared anti-type II grass carp reovirus VP56 protein recombinant rabbit monoclonal antibody has strong specificity and titer, can specifically recognize the virus VP56 protein in a natural state in tissues and cells, has high affinity of the antibody in recognizing and combining the virus VP56 protein, has strong anti-interference capacity, and is suitable for establishing a type II grass carp reovirus diagnosis technology with high specificity, high sensitivity and high accuracy.

The application belongs to the technical field of antigen protein and antibody preparation and discloses a Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody, antigen protein and a preparation method thereof. The application obtains recombinant expression of the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein, obtains the Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody after immunizing mice, and provides an advantageous tool for the research on the Linopneus Genomic Differentiation Factor-8 / 11 protein level. The application also successfully obtains a Linopneus Genomic Differentiation Factor-8 / 11 antigen protein recombinant expression vector, and can obtain the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein. The application also provides a Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence, fills the blank of related research, and the primer pair provided by the application can amplify the above Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence through PCR, and can be used for other related research.

The invention belongs to the technical field of biology, and discloses an influenza HA protein recombinant turkey herpesvirus strain, the preservation number of the recombinant turkey herpesvirus strain is CGMCC No.46642, and the recombinant turkey herpesvirus strain is preserved in China General Microbiological Culture Collection Center; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is July 23, 2025. The recombinant turkey herpesvirus has the characteristics that: 1, the recombinant turkey herpesvirus has good safety and immunogenicity, can induce strong body fluid and cellular immune response, can realize early immunization through intraembryonic or 1-day-old inoculation, and reduces interference of maternal antibodies; and 2, the HA gene of the H7 subtype AIV and the virus HA gene of the H5 subtype AIV are inserted into the virus strain, the two genes are independently screened by the applicant, and after being loaded on an HVT vector, the HA gene and the virus HA gene can show relatively comprehensive protection aiming at the corresponding subtype. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

The invention discloses a preparation technology of a soybean drawing protein recombinant bamboo shoot, which comprises the following steps: firstly, selecting a soybean drawing protein and a dried bamboo shoot raw material; soaking the textured soybean protein, cutting the textured soybean protein into required shapes, soaking the dried bamboo shoots, and cutting the dried bamboo shoots into proper sizes; uniformly mixing the two according to a certain proportion for seasoning; kneading and forming, and loading into a fixed mold; quick-freezing and demolding at the temperature of minus 37 DEG C or below; and frying, shaping, re-frying, taking out, cooling, packaging, sterilizing at high temperature, and warehousing after passing inspection. The soybean drawing protein recombinant bamboo shoot adopting the technical process disclosed by the invention can realize industrial batch production, is crisp and tender in taste, has a muscle texture, is convenient to produce and process, is matched with seasoning technologies of different proportions, ensures the taste diversity, can be used as a snack for resting, and meets the requirements of modern pace of life.

The invention discloses application of Cryptosporidium cgd3_4260 protein as an oocyst wall exocyst marker protein. The inventors have found through research that a Cryptosporidium protein with an amino acid sequence as shown in SEQ ID NO.1 in the sequence table is an oocyst wall exocyst protein, which is located on the outer surface of the Cryptosporidium oocyst wall. The inventors have confirmed the feasibility of using the protein as a detection antigen. The antibody of the recombinant protein (sequence as shown in SEQ ID NO.3 and 5) can be used for immunological detection of unbroken Cryptosporidium oocysts (live Cryptosporidium oocysts), and the invention also provides a specific polypeptide of the protein.

Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and / or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and / or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).

The invention belongs to the technical field of recombinant rice, and particularly relates to plant type high-protein recombinant rice and a preparation method thereof. The recombinant rice is obtained through material mixing, granulation forming and drying, in the material mixing step, raw materials are mixed, and the raw materials comprise wheat protein and at least one of rice protein and pea protein isolate. The recombinant rice prepared by the invention is composed of plant proteins, the specific addition amount and the use proportion of the plant proteins are adjusted, and the proportion of wheat protein as a main component and other plant proteins is defined, so that the effect of relatively high nutritional value is achieved, and the recombinant rice can be balanced in nutrition, rich in nutritional ingredients and relatively comprehensive in nutritional value; and meanwhile, the corresponding high-protein recombinant rice is successfully prepared by combining the innovative adjustment and optimization of the using method and the synergistic effect. The obtained high-protein rehydrated rice is high in nutritional value, is beneficial to improving satiety, and is more suitable for weight-losing people, body-building people and postoperative patients to eat.