6 results about "Protein recombinants" patented technology

The application discloses application of a micro cryptosporidium cgd6_660 protein as an oocyst outer wall marker protein, and discloses that the inventors find that the cryptosporidium cgd6_660 protein is an oocyst outer wall protein, and the protein is located on the outer surface of the cryptosporidium oocyst wall. The inventors confirm the feasibility of the protein as a detection antigen. The antibody of the protein recombinant protein (as shown in a sequence table SEQ ID N0.2) can be used for immunological detection of unbroken cryptosporidium oocysts (live cryptosporidium oocysts), and the application further provides a specific polypeptide of the protein.

The invention discloses an anti-muscovy duck parvovirus VP3 protein recombinant nano antibody, an expression engineering strain and application thereof, and belongs to the field of biology. The anti-Muscovy duck parvovirus VP3 protein recombinant nano antibody is obtained by screening through technologies of computational biology, molecular docking simulation, deep learning and the like. The method comprises the following steps: firstly, screening a heavy-chain antibody variable region sequence VP3-Nb66, then constructing a recombinant eukaryotic expression plasmid by adopting the heavy-chain antibody variable region sequence VP3-Nb66, and further constructing a recombinant expression engineering strain by adopting the recombinant eukaryotic expression plasmid. And further preparing the Muscovy duck parvovirus VP3 protein recombinant nano antibody by adopting the recombinant expression engineering strain. The yield of the recombinant nano antibody reaches 1.01 mg / L, the ELISA result OD450 is equal to 1.66, and the recombinant nano antibody has remarkable binding activity and can be used for preparing a nano antibody preparation for detecting the muscovy duck parvovirus disease.

The invention discloses an HEK293 cell capable of stably expressing pigeon adenovirus I type Fiber2 protein as well as a construction method and application of the HEK293 cell. A pigeon adenovirus I type Fiber2 protein recombinant expression vector is constructed and introduced into an HEK293 adherent cell to obtain a cell QYH-PAF2-A1 capable of expressing the pigeon adenovirus I type Fiber2 protein, and the cell QYH-PAF2-A1 is domesticated into a suspension cell QYH-PAF2-A1. The cell suspension growth state is good, the passage is stable, and the Fiber2 protein can still be stably expressed after 10 generations of continuous culture; then, the Fiber2 protein expressed by the cell is prepared into a subunit vaccine, the protection rate of the subunit vaccine reaches 80% through an immune challenge test, and pigeon adenovirus I type infection can be effectively prevented and controlled. The suspension cell constructed by the invention can be applied to research and development of pigeon adenovirus I type subunit vaccines, preparation of diagnostic reagents and basic research of virus invasion mechanisms, and has a wide application prospect.

The invention relates to the technical field of molecular biology and immunology, in particular to a bovine herpesvirus type 4 gb protein recombinant expression method and application thereof.The 104-384aa segment (the amino acid sequence is shown as SEQ ID NO.2) of bovine herpesvirus type 4 gb protein is selected and constructed to a pet22b / pet32a escherichia coli expression vector, after induced culture and ultrasonication, Ni-NTA gradient elution purification is conducted, and the recombinant expression vector is obtained. The recombinant gb protein with the molecular weight of about 48.5 kDa is obtained. The recombinant protein is used for preparing mouse antiserum, and the titer of the antiserum is not lower than 1: 128000 through ELISA (enzyme-linked immuno sorbent assay) detection; the purity of the purified antiserum meets experimental requirements, and the antiserum can specifically recognize a target antigen. The method solves the problems of poor protein dissolution and low antiserum titer in the traditional method, is controllable in process and high in cost effectiveness, and provides a reliable tool for molecular detection and immunoassay of the bovine herpesvirus type 4.

The application belongs to the technical field of antigen protein and antibody preparation and discloses a Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody, antigen protein and a preparation method thereof. The application obtains recombinant expression of the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein, obtains the Linopneus Genomic Differentiation Factor-8 / 11 polyclonal antibody after immunizing mice, and provides an advantageous tool for the research on the Linopneus Genomic Differentiation Factor-8 / 11 protein level. The application also successfully obtains a Linopneus Genomic Differentiation Factor-8 / 11 antigen protein recombinant expression vector, and can obtain the Linopneus Genomic Differentiation Factor-8 / 11 antigen protein. The application also provides a Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence, fills the blank of related research, and the primer pair provided by the application can amplify the above Linopneus Genomic Differentiation Factor-8 / 11 gene coding region full sequence through PCR, and can be used for other related research.

Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and / or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and / or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).