A recombinant virus comprising BPI gene and a pharmaceutical composition containing the same and uses thereof

A technology for recombining viruses and genes, which can be used in viruses, gene therapy, genetic engineering, etc., and can solve the problems of gene therapy for unseen bacterial infections and low gene transfer efficiency.

CN100529065CActive Publication Date: 2009-08-19XIAMEN LIANHEANJIN BIOTECH CO LTD +1
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2009-08-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention discloses a recombinant virus, which comprises a viral vector, and a gene construct selected from the following: 1) a human BPI gene or a functional fragment gene thereof, or a degenerate Sequence thereof, and 2) a chimeric gene comprising a human BPI gene or a functional fragment gene thereof, or a degenerate Sequence thereof, wherein the chimeric gene further comprises a Fc constant region gene or its allelic gene of a heavy chain of a human immunoglobulin in the 3' end of the human BPI gene or the functional fragment gene thereof. This invention also discloses the use of the gene construct comprising the human BPI gene or functional fragment gene thereof in the preparation of the pharmaceutical composition of gene therapy of the GNB and / or GNB like pathogen infection in Mammal. The present invention further provides a gene therapy method of the GNB and / or GNB like pathogen infection using the recombinant virus or the pharmaceutical composition.
Need to check novelty before this filing date? Find Prior Art

Description

field of invention

[0001] The invention relates to the field of gene therapy for diseases infected by Gram-negative bacteria (GNB) and / or GNB-like pathogens in mammals. Specifically, the present invention relates to a recombinant virus comprising a viral vector and a gene construct selected from the group consisting of: 1) a human BPI gene or a functional fragment gene thereof, or a degenerate sequence thereof; and 2) a human BPI gene or Its functional fragment gene, or a chimeric gene of its degenerate sequence, wherein the 3' end of the human BPI gene or its functional fragment gene is further connected with the human immunoglobulin heavy chain constant region Fc gene or its allelic gene. The present invention also relates to the use of the gene construct containing the human BPI gene or its functional fragment gene for the preparation of a pharmaceutical composition for gene therapy of mammalian GNB and / or GNB-like pathogen infection diseases. The present invention also re...

Examples

Embodiment Construction

[0019] The purpose and implementation of the present invention will be further elaborated below, and those skilled in the art will be obvious to those skilled in the art to the scope, content and advantages involved in the present invention; .

[0020] One aspect of the present invention provides a recombinant virus comprising a viral vector and a gene construct selected from the following:

[0021] 1) human BPI gene or its functional fragment gene, or its degenerate sequence; and

[0022] 2) A chimeric gene containing the human BPI gene or its functional fragment gene, or its degeneracy sequence, wherein the human immunoglobulin heavy chain constant region Fc gene or its allelic genes.

[0023] As a gene therapy carrier, viral vectors have the advantages of high gene transfer efficiency. Retroviral vectors and adenoviral vectors were widely used earlier in clinical research of gene therapy. However, they have limitations, such as the ability to induce immune responses in t...