An apparatus and method for testing the fat content in aquatic organisms

Abstract

The present invention discloses a device for testing the fat content in aquatic organisms, which includes a tissue homogenization and reagent delivery system, a filtration and extraction system, a heating and reagent recovery system, a weighing system, and an automatic control and display system, as well as a method for testing the fat content in aquatic organisms by using this device. The present invention changes the previous high costs of instrument equipment and the previous time-consuming and cumbersome fat extraction methods, providing technical support for determining the fat content in aquatic organisms.

Description

Technical Field

[0001] The present invention relates to the technical field of formulating water quality criteria for human health, and more specifically, to a device and method for measuring the fat content in aquatic organisms. Background Art

[0002] The fat in freshwater aquatic organisms contains a large amount of highly nutritious unsaturated fatty acids. Unsaturated fatty acids have various physiological functions such as regulating blood lipids, clearing blood clots, and anti - inflammation. They can also nourish the brain, enhance the body's immunity, etc. Increasing the intake of fat in aquatic organisms can promote growth and development, reduce the occurrence of diseases, and is beneficial to the development of human health. However, pollutants, especially non - ionic organic compounds, are prone to enrichment and accumulation in the fat of aquatic organisms. Therefore, along with the intake of aquatic organism fat, various pollutants therein are also ingested into the human body.

[0003] With the improvement of living standards, people pay more and more attention to diet nutrition and health, and have an increasing demand for the ingestion of aquatic organisms containing fat. Correspondingly, the intake risk of pollutants is also getting higher and higher. Therefore, China has carried out research and formulation work on water quality criteria for protecting human health and has promulgated national standards. However, in terms of the fat content parameters of edible aquatic organisms involved in human health criteria, there is still a lack of direct relevant fat content parameters, testing methods, and testing devices for various aquatic organisms. At the same time, the fat in aquatic organisms is affected by various factors, not only related to geographical location, but also related to feeding habits, seasons, climate changes, and fish species. Therefore, in order to formulate human health criteria, data on the fat content of aquatic organisms in each region are needed, and a large number of tests on the fat content of aquatic organisms also need to be carried out.

[0004] Therefore, how to provide a convenient, fast, simple, and efficient device and method for measuring the fat content in aquatic organisms is an urgent problem for those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention changes the previous high - cost instrument and equipment and time - consuming and cumbersome fat extraction methods, and provides a device for automatically measuring the fat content in aquatic organisms, providing a basis for determining the fat content in aquatic organisms.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A device for measuring the fat content in aquatic organisms, comprising

[0008] Tissue homogenate and reagent delivery system, the tissue homogenate and reagent delivery system includes a reagent storage device, a homogenizer, and an inductive moisture meter, the reagent storage device is connected to the homogenizer through a pipeline, and the inductive moisture meter is located inside the homogenizer;

[0009] Filtration and extraction system, the filtration and extraction system includes a porous funnel and a separating funnel, the porous funnel is located at the bottom of the homogenizer, and the separating funnel is connected to the liquid outlet of the porous funnel;

[0010] Heating and reagent recovery system, the heating and reagent recovery system includes an extraction cup, a reagent recovery device, a heating device, and a condensing device, the extraction cup is located at the liquid outlet of the separating funnel, and the extraction cup is connected to the reagent recovery device through a pipeline, and the reagent recovery device is connected to the reagent storage device;

[0011] Weighing system, the weighing system is used for weighing organisms and the extraction cup;

[0012] And an automatic control and display system, the automatic control and display system is used for setting the parameters and specific operation procedures required by the whole system, and for receiving and displaying the measurement results.

[0013] Preferably, in the above device for testing the fat content in aquatic organisms, the reagent storage device consists of three storage bottles, which are respectively used for storing distilled water, chloroform, and methanol, and the three storage bottles are all connected to the homogenizer through pipelines.

[0014] The beneficial effects of the above technical solution are: Other solvents such as ether or petroleum ether can also be replaced in the reagent storage device according to actual situations. Under the conditions of standard reagents (i.e., distilled water, chloroform, and methanol), the system does not need to be set and can automatically complete the test. When using other reagents, it needs to be reset through the automatic control and display system to complete the test. The extraction effect of these three reagents is better, the fat is extracted more cleanly, and the fat loss is reduced.

[0015] Preferably, in the above device for testing the fat content in aquatic organisms, a nozzle is provided at one end of the pipeline located in the homogenizer, and the nozzle is connected to three conduits controlled by valves, which respectively correspond to the three storage bottles.

[0016] The beneficial effects of the above technical solution are: According to the set liquid addition volume, the nozzle will spray the corresponding amount of solvent into the homogenizer according to the set solvent ratio of the experiment to wash the four walls and bottom of the homogenizer.

[0017] Preferably, in the above device for testing the fat content in aquatic organisms, a stirring cutter head is provided inside the homogenizer.

[0018] The beneficial effects of the above technical solution are as follows: The stirring cutter head stirs the fish body according to experiments, and the rotation speed of the stirring cutter head can be adjusted through system settings.

[0019] Preferably, in the above device for testing the fat content in aquatic organisms, the probe of the inductive moisture analyzer is placed at the middle position at the bottom of the stirring cutter head, and the probe is fixed by attaching to the stirring cutter head.

[0020] The beneficial effects of the above technical solution are as follows: As the stirring cutter head rotates, the probe of the inductive moisture analyzer will automatically extend into the aquatic biological tissue to detect the moisture of the aquatic biological tissue and display it on the automatic control screen display system.

[0021] Preferably, in the above device for testing the fat content in aquatic organisms, the bottom of the homogenizer is composed of two stainless steel semi-circles, and the opening and closing of the two stainless steel semi-circles are automatically realized by system control.

[0022] The beneficial effects of the above technical solution are as follows: After homogenization, the two semi-circles automatically separate to both sides according to the electrical signal, and the aquatic biological tissue and liquid will flow into the funnel of the bottom filtration and extraction system, and then the two semi-circles close immediately.

[0023] Preferably, in the above device for testing the fat content in aquatic organisms, a sensor for controlling the switch of the separating funnel and a detection rod for detecting liquid transformation are provided at the glass piston of the separating funnel.

[0024] The beneficial effects of the above technical solution are as follows: The separating funnel divides chloroform and methanol into two layers. The glass piston can control the switch of the separating funnel according to the sensor, and the detection probe can detect the transformation of the two liquids. When the lower liquid flows out completely into the extraction cup and methanol liquid appears, the glass piston automatically closes. When the extraction cup is weighed, the glass stopper automatically opens to release methanol.

[0025] The present invention also discloses a method for testing the fat content in aquatic organisms, which includes the following steps:

[0026] (1) Put the processed aquatic biological tissue into the weighing system to weigh it, and the weighing value is displayed at the position of the body weight of the organism in the automatic control screen display system;

[0027] (2) Send the aquatic biological tissue into the homogenizer, set parameters according to the automatic control screen display system, and control the opening of the inductive moisture analyzer through the automatic control screen display system to measure the moisture content of the aquatic biological tissue itself, and then automatically calculate the water content of the aquatic organism according to the weight and moisture content of the aquatic biological tissue;

[0028] (3) Add distilled water, chloroform, and methanol to the homogenizer according to the water content of aquatic organisms for homogenization. The chloroform-methanol mixture extraction method can effectively extract bound lipids from tissues and is particularly suitable for polar fatty foods rich in phospholipids.

[0029] (4) The homogenized material flows from the bottom onto a porous funnel with filter paper and is suction-filtered through a pump. The liquid flowing out of the porous funnel directly flows into the lower separating funnel and is left to stand for layering.

[0030] (5) The lower chloroform layer flows into the extraction cup, and the chloroform is heated and evaporated. The chloroform gas flows through the conduit into the reagent recovery device. When the system detects that no chloroform is flowing into the conduit, the extraction cup is sent to the weighing system for weighing, and the value is recorded according to the automatic control display system.

[0031] (6) The system automatically opens the valve of the separating funnel to let methanol flow into the extraction cup. The methanol is evaporated, and the methanol flows through the conduit into the reagent recovery device. The wastewater and residue are then discharged into the waste collection bag for centralized treatment.

[0032] (7) Based on the fat mass measured in step (5) and the weight of the biological tissue measured in step (1), the fat content is obtained and the calculation result is displayed on the automatic control display system.

[0033] Preferably, in the above method for testing the fat content in aquatic organisms, step (3) specifically includes:

[0034] A. Set the volumes of the solvents chloroform and methanol added by the system for the first time to 5 / 4 water content and 5 / 2 water content respectively, and set the homogenization time to 2 min.

[0035] B. Set the volume of the solvent chloroform added by the system for the second time to 5 / 4 water content, and set the homogenization time to 30 s.

[0036] C. Set the volume of the solvent water added by the system for the third time to 5 / 4 water content, and set the homogenization time to 30 s.

[0037] The beneficial effects of the above technical solution are as follows: Chloroform is used to dissolve lipid components, and methanol can break the lipids combined with proteins. After adding water, small molecule substances such as polysaccharides and amino acids in the sample are dissolved in the methanol-water phase. Adding methanol for the first time can break the lipids combined with proteins in the sample, and chloroform dissolves the lipid components. Since chloroform is denser than methanol and immiscible, chloroform is in the lower layer and methanol is in the upper layer. Adding chloroform again for the second time can extract the lipids in the sample again; adding water for the third time is used to dissolve small molecule substances such as polysaccharides and amino acids in the sample. Because water is miscible with methanol and immiscible with chloroform, water and methanol are in the upper layer and chloroform is in the lower layer.

[0038] Preferably, in the above method for testing the fat content in aquatic organisms, in step (4), the chloroform with a volume flowing out from the wall of the homogenizer being 15 / 8 of the water content is used to rinse the stirrer and the residue.

[0039] The beneficial effects of the above technical solution are as follows: on the one hand, it is to extract fat to the greatest extent and reduce the residue of fat on the container wall and the residue. On the other hand, it is also to save reagents. Through multiple experiments, it is found that the residual fat recovered under this ratio accounts for about 6% of the total extracted fat.

[0040] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a device and method for automatically testing the fat content in aquatic organisms, which changes the previous high cost of instrument equipment and the time-consuming and cumbersome fat extraction method, and provides technical support for determining the fat content in aquatic organisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0042] Figure 1 The drawings are schematic diagrams of the frame structure of the device of the present invention;

[0043] Figure 2 The drawings are schematic diagrams of the overall structure of the device of the present invention;

[0044] Figure 3 The drawings are schematic diagrams of the structure at the bottom of the homogenizer of the present invention;

[0045] Figure 4 The drawings are schematic diagrams of the process flow of the device of the present invention.

[0046] In the figure:

[0047] 1 is a motor, 2 is a stirring cutter head, 3 is a homogenizer, 4 is a pipeline, 5 is a reagent storage device, 6 is a probe, 7 is an automatic control display system, 8 is a porous funnel, 9 is a separating funnel, 10 is a pump, 11 is a glass piston, 12 is a detection rod, 13 is an extraction cup, 14 is a reagent recovery device, 15 is a condensation device, 16 is a weighing meter, 17 is a heating device. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0048] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0049] The device for testing the fat content in aquatic organisms provided by the present invention is mainly used for automatically extracting fat and obtaining the fat content in each organism, and can achieve the purpose of automatically calculating the fat content of the measured organism.

[0050] The device for testing the fat content in aquatic organisms provided in this embodiment includes a tissue homogenization and reagent delivery system, a filtration and extraction system, a heating and reagent recovery system, a weighing system, and an automatic control and display system.

[0051] Among them, the tissue homogenization and reagent delivery system first measures the water content of the organism through the inductive moisture meter set therein, calculates the amount of solvent to be injected according to the water and the proportion of each solvent, and quantitatively delivers the reagent into the homogenizer through a pipeline. The stirring cutter head homogenizes the organism according to the set time.

[0052] The filtration and extraction system first filters the organism after homogenization according to filter layers with different pore sizes, and then separates the two immiscible liquids formed after filtration in a separating funnel.

[0053] The heating and reagent recovery system is used to evaporate the solvent left in the separating funnel by heating, so as to extract fat by evaporating the solvent, and recycle the reagent through a recovery pipeline for repeated use.

[0054] The weighing system is used to weigh the organism before tissue homogenization and weigh the fat after heating and evaporation.

[0055] The automatic control and display system device sets the parameters required by the system according to the touch screen and the specific operation process of the experiment.

[0056] The device for testing the fat content in aquatic organisms provided in the embodiment of the present invention is specifically as follows:

[0057] 1. Tissue homogenization and reagent delivery system

[0058] The system mainly consists of a motor 1, a stirring cutter head 2, a homogenizer 3, an inductive moisture detector, a pipe 4 with a nozzle, and a reagent storage device 5. A cylindrical motor 1 with a height of 15 cm and a radius of 5 cm is placed on the upper part. A homogenizer 3 with a height of 30 cm and a radius of 10 cm is fixed to the motor 1. In the middle of the homogenizer 3 is a stirring cutter head 2 whose rotation speed can be set according to the system. The kinetic energy of the stirring cutter head 2 is provided by the motor 1. The stirring cutter head 2 is fixed at the upper part to stir aquatic organisms. The probe 6 of the inductive moisture detector is located in the middle of the stirring cutter head 2. When the instrument is turned on, the probe 6 will automatically extend towards the organism to detect the moisture in the tissue of aquatic organisms and display it on the automatic control screen display system 7. The nozzle is located 5 cm from the top of the stainless-steel homogenizer 3. According to the set liquid volume, the nozzle will spray the corresponding amount of solvent into the homogenizer 3 according to the proportion of the solvent added in the set experiment to wash the inner walls and bottom of the stainless-steel homogenizer 3. Three pipes are connected to the nozzle. The valves of the corresponding pipes will be opened according to the reagent requirements. The total length of each pipe is 1 meter. The reagents are located in three 4L glass bottles, containing distilled water, chloroform, and methanol respectively. The bottom of the homogenizer 3 consists of two stainless-steel semi-circles. After homogenization, the two semi-circles separate to both sides, and the organisms and liquid will flow into the funnel of the bottom filtration and extraction system, and then the two semi-circles close immediately.

[0059] 2. Filtration and Extraction System

[0060] This system mainly consists of a ceramic porous funnel 8, a separating funnel 9, and a pump 10. The ceramic porous funnel 8 is at the bottom of the homogenizer 3. The inner diameter of the porous funnel 8 is 20 cm. It can automatically change the Whatman No.1 filter paper by grasping the handle. The homogenized liquid flows through the funnel, and the funnel sucks chloroform and methanol into the lower separating funnel 9 by the suction of the pump. The pear-shaped glass separating funnel 9 separates chloroform and methanol into two layers. The glass piston 11 can control the opening and closing of the separating funnel 9 according to the sensor. There is a detection rod 12 above the glass piston 11 to detect the change of the two liquids. When the liquid flowing into the extraction cup 13 of the heating and reagent recovery system at the lower part runs out and methanol liquid appears, the glass piston 11 automatically closes.

[0061] 3. Heating and Reagent Recovery System

[0062] The system consists of an extraction cup 13, a reagent recovery device 14, a condensation device 15, and a heating device 17. The liquid flows through the separating funnel 9 into the lower extraction cup 13. At the set experimental heating temperature, the heating device 17 can evaporate the liquid solvent into the reagent recovery device 14 for storing chloroform. When there is no chloroform flowing in the conduit, the system will transport the extraction cup 13 into the weighing system 16 for weighing, automatically record the mass of the fat, and display it on the automatic control screen display system 7. Then, the glass piston 11 will automatically open to release methanol, and continue to evaporate at the set experimental heating temperature into the reagent recovery device 14 for storing methanol. There is a pipe with a valve in the upper left part of the extraction cup 13. The evaporated solvent gas passes through the pipe and is condensed by the condensation device 15 and then turns into a liquid and flows into the reagent recovery device 14 at the bottom of the pipe to recycle the used solvent. The recycled reagent can be re-transported back to the reagent storage device 5 for storing the solvent through the pipe for reuse.

[0063] 4. Weighing System

[0064] A weighing meter 16 is set at the bottom of the system. Before the aquatic biological tissue is put into the system, it is first weighed in the weighing system. At this time, the automatic control screen display system 7 will automatically record the mass of the organism, and then the system will transport the aquatic biological tissue into the homogenizer 3 of the tissue homogenization and reagent delivery system. When the chloroform in the extraction cup 13 has completely evaporated, the system will send the dried fat to the weighing meter 16 for weighing, and display the specific value after the fat mass on the automatic control screen display system 7.

[0065] 5. Automatic Control Screen Display System Equipment

[0066] The entire experimental process can be controlled according to the automatic control screen display system 7. In the automatic control screen display system 7, the proportion of the solvent added in the experiment, the time for each step of the experiment, the experimental heating temperature, the display of the fat mass, and the calculation of the fat content can be set. The automatic control screen display system 7 is 30 cm long, 20 cm wide, and 8 cm high, and the experimental process can be set through the touch screen.

[0067] The method for testing the fat content in aquatic organisms using the above device is as follows:

[0068] Put the fish body from which the fish scales have been removed, the fish head has been removed, and the weight has been measured into the weighing system. The system will automatically weigh it and display the specific value of the organism's body weight on the automatic control screen display system. Immediately afterwards, the system will send it into the blender;

[0069] Set parameters according to the automatic control display system. Turn on the switch of the inductive moisture analyzer in the automatic control display system to measure the moisture content of the fish body. The system automatically calculates the water content of the fish body based on the body weight and moisture content of the organism. The volumes of chloroform and methanol added by the system for the first time are automatically set to 5 / 4 water content and 5 / 2 water content respectively (for example, if the measured water content is 80 ml, then according to the ratio, the system automatically calculates that the chloroform and methanol added for the first time are 100 ml and 200 ml respectively), and the homogenization time is automatically set to 2 min. The volume of chloroform added by the system for the second time is automatically set to 5 / 4 water content (for example, if the system has calculated the water content to be 80 ml, then it automatically calculates that 100 ml of chloroform needs to be added according to the ratio), and the homogenization time for this step is automatically set to 30 s. The volume of water added by the system for the third time is automatically set to 5 / 4 water content (for example, if the system has calculated the water content to be 80 ml, then it automatically calculates that 100 ml of water needs to be added according to the ratio), and the homogenization time is set to 30 s. When using other non-recommended solvents, the ratio of each solvent and the homogenization time can be manually set through the control panel on the automatic control display system;

[0070] The homogenized substance flows from the bottom of the homogenizer to a Coors No. 3 porous funnel with Whatman No. 1 filter paper placed on it, and is suction filtered through a pump. Set the system so that the amount of chloroform flowing out from the wall of the homogenizer after filtration is 15 / 8 of the water content (the system automatically calculates the volume of chloroform based on the water content. For example, if the water content is 80 ml, the system automatically calculates the chloroform volume to be 150 ml), wash the wall of the homogenizer and then let it flow out from the funnel to wash the stirrer and residues;

[0071] The liquid flowing out from the porous funnel directly flows into the lower separating funnel. Set the static time in the separating funnel to 2 minutes through the automatic control display system. The lower chloroform layer flows into the extraction cup, and the chloroform is evaporated according to the first heating temperature set by the automatic control display system. The chloroform gas first flows through a conduit into the device for recovering chloroform. When the system detects that no chloroform flows into the conduit, the system will send the extraction cup to the weighing system for weighing, and display the specific value after the fat mass on the automatic control display system. Next, the system automatically opens the valve of the separating funnel, allows methanol to flow into the extraction cup, and evaporates the methanol according to the second heating temperature set by the automatic control display system. The methanol flows back through the conduit to the methanol reagent recovery device for recycling. The solvents in the recovery device will be re-transported through pipelines back to the corresponding storage devices for reuse;

[0072] According to the calculation method set in the system, that is, dividing the fat mass measured by the device by the body weight of the organism, the fat content can be obtained. The automatic control display system automatically calculates the fat content and displays the calculation result.

[0073] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. For the same or similar parts among the various embodiments, reference can be made to each other. For the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple. For related parts, reference can be made to the description in the method section.

[0074] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for testing the fat content in aquatic organisms, characterized in that, the device for testing the fat content in aquatic organisms includes a tissue homogenization and reagent delivery system, and the tissue homogenization and reagent delivery system includes a reagent storage device, a homogenizer and an inductive moisture meter. The reagent storage device is connected to the homogenizer through a pipeline, and the inductive moisture meter is located inside the homogenizer; a filtration and extraction system, the filtration and extraction system includes a porous funnel and a separating funnel. The porous funnel is located at the bottom of the homogenizer, and the separating funnel is connected to the liquid outlet of the porous funnel; a heating and reagent recovery system, the heating and reagent recovery system includes an extraction cup, a reagent recovery device, a heating device and a condensing device. The extraction cup is located at the liquid outlet of the separating funnel, and the extraction cup is connected to the reagent recovery device through a pipeline. The reagent recovery device is connected to the reagent storage device; a weighing system, the weighing system is used for weighing the organism and the extraction cup; and an automatic control and display system, the automatic control and display system is used for setting the parameters required by the whole system and the specific operation process, and for receiving and displaying the measurement results; the reagent storage device consists of three storage bottles, which are respectively used for storing distilled water, chloroform and methanol, and the three storage bottles are all connected to the homogenizer through pipelines; a nozzle is arranged at one end of the pipeline located inside the homogenizer; a sensor for controlling the switch of the separating funnel and a detection probe for detecting liquid change are arranged at the glass piston of the separating funnel; the method for testing the fat content in aquatic organisms includes the following steps: (1) Put the processed aquatic organism tissue into the weighing system for weighing, and the weighing value is displayed at the position of the weight of the biological tissue in the automatic control and display system; (2) Automatically send the aquatic organism tissue into the homogenizer, set the parameters according to the automatic control and display system, and control the opening of the inductive moisture meter through the automatic control and display system to measure the moisture content of the aquatic organism tissue, and then automatically calculate the water content of the aquatic organism tissue according to the weight and moisture content of the aquatic organism tissue; (3) Automatically add chloroform and methanol into the homogenizer according to the water content of the aquatic organism tissue for homogenization; (4) The homogenized substance flows from the bottom to the porous funnel with filter paper, and is suction-filtered by a pump. The liquid flowing out of the porous funnel directly flows into the lower separating funnel and stands for stratification; (5) The lower chloroform layer flows into the extraction cup, the chloroform is heated and evaporated, and the chloroform gas flows into the reagent recovery device through a conduit. When the system detects that no chloroform flows into the conduit, the extraction cup is sent to the weighing system for weighing, and the automatic control and display system records the value; (6) The system automatically opens the valve of the separating funnel, allows methanol to flow into the extraction cup, evaporates the methanol, and the methanol flows into the reagent recovery device through a conduit; (7) According to the fat mass measured in step (5) and the weight of the biological tissue measured in step (1), obtain the fat percentage content, and the calculation result is displayed on the automatic control and display system; Step (3) specifically includes: A. Set the volumes of chloroform and methanol added to the system for the first time to 5 / 4 water content and 5 / 2 water content respectively, and set the homogenization time to 2 min; B. Set the volume of chloroform added to the system for the second time to 5 / 4 water content, and set the homogenization time to 30 s; C. Set the volume of water added to the system for the third time to 5 / 4 water content, and set the homogenization time to 30 s. In step (4), the stirrer and residues are rinsed with chloroform with a volume of 15 / 8 of the water content ejected from the nozzle after filtration.

2. A method for testing the fat content in aquatic organisms according to claim 1, characterized in that a stirring cutter head is provided in the homogenizer.

3. A method for testing the fat content in aquatic organisms according to claim 1, characterized in that the bottom of the homogenizer is composed of two stainless steel semi-circles.

4. A method for testing the fat content in aquatic organisms according to claim 1, characterized in that under standard conditions, steps (1)-(7) are automatically completed through the automatic control screen display system, and under non-standard conditions, parameters are set through the automatic control screen display system to complete steps (1)-(7).

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