Combined microfluidic chip

Abstract

The present invention relates to the technical field of microfluidic chips, and provides a combined microfluidic chip, including a disc-shaped chip body and an adapter, wherein the adapter is used to fix the disc-shaped chip body on a detection instrument, and the disc-shaped chip body is composed of a plurality of sectors, each of which is provided with a chip monomer, each of which is provided with a fixing structure that cooperates with the adapter, a connection structure that cooperates with chip monomers of other sectors, and a functional area for reaction detection, and both the front and back of the chip monomer are provided with a packaging layer in the functional area. In the present invention, a plurality of chip monomers can be freely combined and assembled into different types of joint inspections, and the connection structure between the chip monomers makes it simple to assemble a plurality of chip monomers or disassemble the disc-shaped chip body, and the adapter solves the problem of adapting the combined microfluidic chip of the present invention to existing detection instruments.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and more specifically to a combined microfluidic chip. Background Art

[0002] Microfluidic devices are usually called microfluidic chips, also known as chip laboratories. They make the basic operating units involved in the fields of biology and chemistry, such as reaction, separation, culture, sorting, and detection, into micro / nano-scale components and integrate them into a micron-scale chip. A network is formed by microchannels, and controllable fluids are used throughout the entire system to achieve various conventional biological or chemical functions.

[0003] The current disc-shaped microfluidic chip can only perform pre-embedded detection and cannot flexibly configure the type of joint detection. For example, a disc-shaped 8-sample chip has a total of 8 sample loading chambers, each with 4 reaction pools, and detection reagents are pre-embedded in the reaction pools. The chip can detect 8 samples at the same time, and each sample can only be jointly tested for 4 fixed detection items, and the type of joint detection cannot be changed by itself. In addition, when the number of samples that can be tested on the chip is greater than the number of samples to be tested, the excess unreacted channels are often wasted in the current disc-shaped microfluidic chip. If the used chip with waste liquid is retained, it will often cause contamination due to poor storage or careless operation during reuse. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a combined microfluidic chip that can freely combine and assemble microfluidic chips of different specifications or pre-embedded with different reagents into different types of joint tests. After the test, all microfluidic chips can be disassembled and the used chips can be discarded.

[0005] The technical solution of the present invention is to provide a combined microfluidic chip, including a disc-shaped chip body and an adapter, the adapter is used to fix the disc-shaped chip body on the detection instrument, the disc-shaped chip body is composed of a plurality of sectors, each sector is provided with a chip monomer, each chip monomer is provided with a fixing structure cooperating with the adapter, a connection structure cooperating with chip monomers of other sectors, and a functional area for reaction detection, and packaging layers are provided in the functional areas on the front and back of the chip monomer.

[0006] Compared with the prior art, the combined microfluidic chip of the present invention has the following advantages: multiple chip monomers can be combined and connected to form a disc-shaped chip body, and the chip monomers can be of the same specification or different specifications, and can be freely combined and assembled into different types of joint inspections; multiple chip monomers can be assembled or the disc-shaped chip body can be disassembled by utilizing the connection structure between the chip monomers; the disc-shaped chip body can be fixed on the detection instrument through an adapter, which solves the adaptation problem of the combined microfluidic chip of the present invention and the existing detection instrument.

[0007] Preferably, the functional area includes a liquid storage area, a microchannel area, and a reaction area. The liquid storage area is provided with a liquid storage cylinder and a sample addition hole, an exhaust hole, and a plurality of reserved grooves connected to the liquid storage cylinder. The microchannel area is provided with a plurality of microfluidic channels connected to the reserved grooves in a one-to-one correspondence. The reaction area is provided with a plurality of reaction pools connected to the microfluidic channels in a one-to-one correspondence. With this structure, the disc-shaped chip body assembled by free combination is consistent with the conventional disc-shaped microfluidic chip structure and can be compatible with existing detection instruments.

[0008] Preferably, a ball valve is provided in each microfluidic channel. With this structure, the ball valve can allow the liquid in the reserved groove to flow into the reaction pool, while preventing the reagent in the reaction pool from flowing out into the liquid storage cylinder, ensuring the normal reaction and avoiding reagent contamination and mutual interference between reactions.

[0009] Preferably, the number of reaction pools, the number of corresponding reserved slots, and the number of microfluidic channels on the disc-shaped chip body are all 32. With this structure, the disc-shaped chip body assembled by free combination is compatible with existing detection instruments.

[0010] Preferably, the number of reaction pools on the chip unit and the corresponding number of reserved slots and the number of microfluidic channels are all K times of 2, and the angle of the sector center angle θ of the sector where the chip unit is located is K times of 22.5, K is an integer and 1≤K≤16. With this structure, one liquid storage cylinder, i.e., the sample adding chamber, can be simultaneously connected to 2, 4, 6, 8, etc., any even number of reaction pools within 32, corresponding to the joint inspection of 2, 4, 6, 8, etc., any even number of fixed detection items within 32, to achieve parallel analysis of single sample and multiple indicators or multiple samples and multiple indicators.

[0011] Preferably, the number of reaction pools on the chip unit and the corresponding number of reserved slots and the number of microfluidic channels are all M times 1, and the angle of the sector center angle θ of the sector where the chip unit is located is M times 11.25, M is an integer and 1≤M≤32. With this structure, one liquid storage cylinder, i.e., the sample adding chamber, can be connected to any number of reaction pools within 32 at the same time, corresponding to any number of fixed detection items within 32 for joint inspection, and realize parallel analysis of single sample multiple indicators, multiple samples multiple indicators, or multiple samples single indicator.

[0012] Preferably, the connection structure between the chip monomers includes a first connection step surface arranged on the front side of the chip monomer and a second connection step surface arranged on the back side of the chip monomer, and the first connection step surface and the second connection step surface between the chip monomers are correspondingly connected. With this structure, the front and back sides of the connection between the chip monomers can be flat, and the assembled disc-shaped chip body also has a flat surface.

[0013] Preferably, at least one connection column is provided on the first connection step surface, at least one connection hole is provided on the second connection step surface, and the connection columns and connection holes between the chip monomers are matched and connected one by one. With this structure, the second connection step surface of the chip monomer corresponds to cover the first connection step surface of the adjacent chip monomer, and the connection column on the first connection step surface extends into the connection hole on the second connection step surface, so that the chip monomers are firmly connected.

[0014] Preferably, the encapsulation layer on the back of the chip unit is a transparent optical film, and the transparent optical film is used to seal the liquid storage cylinder, the reserved groove, the microfluidic channel and the reaction pool. With this structure, the chip unit only needs to manufacture structures such as the liquid storage cylinder, the reserved groove, the microfluidic channel and the reaction pool, and the sealing of the liquid storage cylinder, the reserved groove, the microfluidic channel and the reaction pool is completed by the transparent optical film, which is easy to produce, and the transparent optical film will not affect the existing detection instrument to perform optical analysis on the reaction results in the chip.

[0015] Preferably, the encapsulation layer on the front side of the chip unit is used to seal the sample loading hole and the exhaust hole. With this structure, the sample loading hole and the exhaust hole are sealed with the encapsulation layer after the sample is loaded, so that the reaction liquid will not be thrown out of the chip when the chip is centrifuged, and the reaction product will always be in a closed environment without leakage and pollution. The encapsulation layer can use an aluminum film that is easy to tear and stick.

[0016] Preferably, the chip monomer is in the shape of a fan ring, and several chip monomers are connected to form a disc-shaped chip body, and the inner ring arcs of all chip monomers are spliced ​​to form the center hole of the disc-shaped chip body; the adapter includes a support platform for supporting the disc-shaped chip body, a support column for limiting the radial movement of the disc-shaped chip body, and at least one buckle for limiting the axial movement of the disc-shaped chip body is arranged on the support platform; the center hole of the disc-shaped chip body is sleeved on the support column, the buckle extends from the center hole, and the disc-shaped chip body is limited between the hook surface of the buckle and the support platform. With this structure, the disc-shaped chip body is sleeved on the support column, the inner wall of the center hole conflicts with the inner wall of the buckle and the side wall of the support column, preventing the disc-shaped chip body from moving radially; the front and back of the disc-shaped chip body conflict with the hook surface of the buckle and the support platform respectively, preventing the disc-shaped chip body from moving axially; at the same time, the hook surface of the buckle and the support platform generate static friction on the front and back of the disc-shaped chip body, preventing the disc-shaped chip body from moving circumferentially.

[0017] Preferably, at least one limiting groove is provided on the inner circular arc of the chip body, and the buckle extends from the central hole and is limited in the limiting groove. With this structure, the buckle prevents the disc-shaped chip body from moving circumferentially by preventing the groove wall of the limiting groove from moving left and right.

[0018] Preferably, an inclined surface is provided between the bottom of the limiting groove and the back of the chip unit; a plurality of guide platforms are also provided on the support platform, and a guide surface inclined downward from the inside to the outside is provided on the top of the guide platform, and a plurality of limiting grooves are correspondingly connected to the buckles, and the inclined surfaces of a plurality of other limiting grooves are against the guide surface. With this structure, the inclined surface of the limiting groove is against the guide surface inclined downward from the inside to the outside, so that the chip unit is tilted and fixed, and the height of the reaction pool is lower than the height of the liquid storage cylinder, which is conducive to the sample liquid flowing from the liquid storage cylinder into the reaction pool during the centrifugation process.

[0019] Preferably, the buckle is elastically connected to the support platform. With this structure, when the center hole of the disc-shaped chip body is sleeved on the support column, the buckle can be pressed against the support column to make both the buckle and the support column extend out of the center hole, and when the buckle is released, the buckle will be in close contact with the disc-shaped chip body to fix the disc-shaped chip body; when the disc-shaped chip body is removed from the support column, the buckle can be pressed against the support column to make both the buckle and the support column detach from the center hole, making it easy to disassemble and assemble the disc-shaped chip body.

[0020] Preferably, the adapter is also provided with an adapting hole for matching with the detection instrument, and a positioning edge is provided on the inner wall of the adapting hole. With this structure, the adapting hole is connected to the detection instrument, and the positioning edge limits the adapter, so that the adapter rotates together with the detection instrument.

[0021] Preferably, the adapter is also provided with an adapting hole for matching with the detection instrument, and a positioning groove is provided on the inner wall of the adapting hole. With this structure, the adapting hole is connected to the detection instrument, and the positioning groove limits the adapter, so that the adapter rotates together with the detection instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic structural diagram of the U2 chip monomer in the combined microfluidic chip of the present invention.

[0023] Figure 2 It is a schematic diagram of the front structure of the U2 chip unit in the combined microfluidic chip of the present invention.

[0024] Figure 3 It is a schematic diagram of the back structure of the U2 chip unit in the combined microfluidic chip of the present invention.

[0025] Figure 4 for Figure 2 Front view of .

[0026] Figure 5 It is a schematic diagram of the structure of the adapter in the combined microfluidic chip of the present invention.

[0027] Figure 6 It is a schematic diagram of the front structure of the adapter in the combined microfluidic chip of the present invention.

[0028] Figure 7 It is a schematic diagram of the back structure of the adapter in the combined microfluidic chip of the present invention.

[0029] Figure 8 It is a schematic structural diagram of the U4 chip monomer in the combined microfluidic chip of the present invention.

[0030] Fig. 9 It is a schematic diagram of the structure of the U8 chip monomer in the combined microfluidic chip of the present invention.

[0031] As shown in the figure: 1. Chip monomer, 1-1. Fixed structure, 1-2. Liquid storage area, 1-3. Liquid storage cylinder, 1-4. Reserved groove, 1-5. Sample addition hole, 1-6. Exhaust hole, 1-7. Microchannel area, 1-8. Microfluidic channel, 1-9. Ball valve, 1-10. Reaction area, 1-11. Reaction pool, 1-12. First connecting step surface, 1-13. Connecting column, 1-14. Second connecting step surface, 1-15. Connecting hole, 1-17. Limiting groove, 1-18. Inclined surface, 2. Adapter, 2-1. Support platform, 2-2. Support column, 2-3. Buckle, 2-4. Guide platform, 2-5. Guide surface, 2-6. Adapter hole, 2-7. Positioning edge, 2-8. Positioning groove, 2-9. Inner wall of buckle, 2-10. Hook surface of buckle. DETAILED DESCRIPTION

[0032] In order to better understand the present application, a more detailed description of various aspects of the present application will be made with reference to the accompanying drawings. It should be understood that these detailed descriptions are only descriptions of exemplary embodiments of the present application, and do not limit the scope of the present application in any way. Throughout the specification, the same figure numerals refer to the same elements.

[0033] In the drawings, the thickness, size and shape of objects have been slightly exaggerated for ease of explanation. The drawings are only examples and are not drawn strictly to scale.

[0034] It should also be understood that the terms "comprises", "includes", "has", "includes", "comprising", when used in this specification, indicate the presence of the described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof. In addition, when a statement such as "...at least one" appears after a list of listed features, it modifies the entire listed features rather than modifying the individual elements in the list.

[0035] like Figures 1 to 4 As shown in , the U2 chip monomer in the combined microfluidic chip of the present invention is in the shape of a fan ring, and is divided into a liquid storage area 1-2, a microchannel area 1-7, and a reaction area 1-10 from the inner ring to the outer ring. The liquid storage area 1-2 is provided with a liquid storage cylinder 1-3 and a sample addition hole 1-5, an exhaust hole 1-6, and two reserved grooves 1-4 connected to the liquid storage cylinder 1-3. The microchannel area 1-7 is provided with two microfluidic channels 1-8 connected to the reserved grooves 1-4 in a one-to-one correspondence. The reaction area 1-10 is provided with two reaction pools 1-11 connected to the microfluidic channels 1-8 in a one-to-one correspondence, and each microfluidic channel 1-8 is provided with a ball valve 1-9. The back of the U2 chip monomer is encapsulated with a transparent optical film, which seals the liquid storage cylinder 1-3, the reserved groove 1-4, the microfluidic channel 1-8, the ball valve 1-9, and the reaction pool 1-11. The front of the U2 chip monomer is encapsulated with an aluminum film, and the sample loading hole 1-5 and the exhaust hole 1-6 are usually sealed with the aluminum film after the sample is loaded. The two sides of the U2 chip monomer are respectively provided with connection structures that cooperate with other chip monomers, including a first connection step surface 1-12 arranged on the front of the U2 chip monomer and a second connection step surface 1-14 arranged on the back of the U2 chip monomer. Two connection columns 1-13 are arranged on the first connection step surface 1-12, and two connection holes 1-15 are arranged on the second connection step surface 1-14. The second connection step surface 1-14 of the U2 chip monomer correspondingly covers the first connection step surface 1-12 of the adjacent U2 chip monomer, and the connection column 1-13 on the first connection step surface correspondingly extends into the connection hole 1-15 of the second connection step surface. 16 pieces of U2 chip monomers can be combined into a complete disc-shaped chip body. Since the first connection step surface 1-12 of the U2 chip monomer is overlapped and covered by the second connection step surface 1-14 of the adjacent U2 chip monomer, the first connection step surface 1-12 must be removed to calculate the angle of the central angle θ of the sector where the U2 chip monomer is located. The angle of the central angle θ of the sector where the U2 chip monomer is located is 22.5 degrees. A limiting groove 1-17 is arranged at the center of the inner ring arc of the U2 chip monomer, and an inclined surface 1-18 is arranged between the bottom of the limiting groove 1-17 and the back of the U2 chip monomer 1. The inner ring arc and the limiting groove 1-17 are the fixed structure 1-1 for the U2 chip monomer to cooperate with the adapter 2.

[0036] like Figures 5 to 7 As shown in the figure, the adapter 2 in the combined microfluidic chip of the present invention includes a support platform 2-1, a support column 2-2 is arranged at the center of the support platform 2-1, the outer diameter of the support column 2-2 is slightly larger than the inner arc radius of the chip monomer in the combined microfluidic chip of the present invention, the height of the support column 2-2 is larger than the thickness of the chip monomer in the combined microfluidic chip of the present invention, and four buckles 2-3 are evenly arranged on the support platform 2-1, and the buckles 2-3 are elastically connected to the support platform 2-1 (such as Figures 5 to 7 As shown in , the hook surface of the buckle 2-3 is slightly higher than the upper bottom surface of the support column 2-2. Four guide platforms 2-4 are also evenly arranged on the support platform 2-1. The inner side surface of the guide platform 2-4 is connected back to back to the cylindrical surface of the support column 2-2. The left and right sides of the guide platform 2-4 are respectively provided with grooves between the cylindrical surface of the support column 2-2, and the top surface of the guide platform 2-4 is provided with a guide surface 2-5 that is inclined downward from the inside to the outside. The four guide platforms 2-4 and the four buckles 2-3 are evenly distributed on the circumference where the support column 2-2 and the support platform 2-1 intersect, and a gap is left between the buckle 2-3 and the cylindrical surface of the support column 2-2. The adapter 2 is also provided with an adapter hole 2-6 for mounting in cooperation with the detection instrument, and a positioning edge 2-7 and a positioning groove 2-8 are provided on the inner wall of the adapter hole 2-6.

[0037] like Figure 8As shown in , the U4 chip monomer in the combined microfluidic chip of the present invention is in the shape of a fan ring, and is divided into a liquid storage area 1-2, a microchannel area 1-7, and a reaction area 1-10 from the inner ring to the outer ring. The liquid storage area 1-2 is provided with a liquid storage cylinder 1-3 and a sample addition hole 1-5, an exhaust hole 1-6, and four reserved grooves 1-4 connected to the liquid storage cylinder 1-3. The microchannel area 1-7 is provided with four microfluidic channels 1-8 connected to the reserved grooves 1-4 in a one-to-one correspondence. The reaction area 1-10 is provided with four reaction pools 1-11 connected to the microfluidic channels 1-8 in a one-to-one correspondence, and each microfluidic channel 1-8 is provided with a ball valve 1-9. The back of the U4 chip monomer is encapsulated with a transparent optical film, which seals the liquid storage cylinder 1-3, the reserved groove 1-4, the microfluidic channel 1-8, the ball valve 1-9, and the reaction pool 1-11. The front of the U4 chip monomer is encapsulated with an aluminum film, and the sample loading hole 1-5 and the exhaust hole 1-6 are usually sealed with the aluminum film after the sample is loaded. The two sides of the U4 chip monomer are respectively provided with connection structures that cooperate with other chip monomers, including a first connection step surface 1-12 arranged on the front of the U4 chip monomer and a second connection step surface 1-14 arranged on the back of the U4 chip monomer. Two connection columns 1-13 are arranged on the first connection step surface 1-12, and two connection holes 1-15 are arranged on the second connection step surface 1-14. The second connection step surface 1-14 of the U4 chip monomer correspondingly covers the first connection step surface 1-12 of the adjacent U4 chip monomer, and the connection column 1-13 on the first connection step surface correspondingly extends into the connection hole 1-15 of the second connection step surface. 8 pieces of U4 chip monomers can be combined into a complete disc-shaped chip body. Since the first connection step surface 1-12 of the U4 chip monomer is overlapped and covered by the second connection step surface 1-14 of the adjacent U4 chip monomer, the first connection step surface 1-12 must be removed to calculate the angle of the central angle θ of the sector where the U4 chip monomer is located. The angle of the central angle θ of the sector where the U4 chip monomer is located is 45 degrees. Two limiting grooves 1-17 are evenly arranged on the inner ring arc of the U4 chip monomer. An inclined surface 1-18 is arranged between the bottom of the limiting groove 1-17 and the back of the U4 chip monomer 1. The inner ring arc and the limiting groove 1-17 are the fixed structure 1-1 for the U4 chip monomer to cooperate with the adapter 2.

[0038] like Fig. 9As shown in , the U8 chip monomer in the combined microfluidic chip of the present invention is in the shape of a fan ring, and is divided into a liquid storage area 1-2, a microchannel area 1-7, and a reaction area 1-10 from the inner ring to the outer ring. The liquid storage area 1-2 is provided with a liquid storage cylinder 1-3 and a sample addition hole 1-5, an exhaust hole 1-6, and 8 reserved grooves 1-4 connected to the liquid storage cylinder 1-3. The microchannel area 1-7 is provided with 8 microfluidic channels 1-8 connected to the reserved grooves 1-4 in a one-to-one correspondence. The reaction area 1-10 is provided with 8 reaction pools 1-11 connected to the microfluidic channels 1-8 in a one-to-one correspondence, and each microfluidic channel 1-8 is provided with a ball valve 1-9. The back of the U8 chip monomer is encapsulated with a transparent optical film, which seals the liquid storage cylinder 1-3, the reserved groove 1-4, the microfluidic channel 1-8, the ball valve 1-9, and the reaction pool 1-11. The front of the U8 chip monomer is encapsulated with an aluminum film, and the sample loading hole 1-5 and the exhaust hole 1-6 are usually sealed with the aluminum film after the sample is loaded. The two sides of the U8 chip monomer are respectively provided with connection structures that cooperate with other chip monomers, including a first connection step surface 1-12 arranged on the front of the U8 chip monomer and a second connection step surface 1-14 arranged on the back of the U8 chip monomer. Two connection columns 1-13 are arranged on the first connection step surface 1-12, and two connection holes 1-15 are arranged on the second connection step surface 1-14. The second connection step surface 1-14 of the U8 chip monomer correspondingly covers the first connection step surface 1-12 of the adjacent U8 chip monomer, and the connection column 1-13 on the first connection step surface correspondingly extends into the connection hole 1-15 of the second connection step surface. Four U8 chip monomers can be combined into a complete disc-shaped chip body. Since the first connection step surface 1-12 of the U8 chip monomer is overlapped by the second connection step surface 1-14 of the adjacent U8 chip monomer, the first connection step surface 1-12 must be removed to calculate the angle of the central angle θ of the sector where the U8 chip monomer is located. The angle of the central angle θ of the sector where the U8 chip monomer is located is 90 degrees. Four limiting grooves 1-17 are evenly arranged on the inner ring arc of the U8 chip monomer, and an inclined surface 1-18 is arranged between the bottom of the limiting groove 1-17 and the back of the U8 chip monomer 1. The inner ring arc and the limiting groove 1-17 are the fixed structure 1-1 for the U8 chip monomer to cooperate with the adapter 2.

[0039] The radius of the inner ring and outer ring of the U2 chip monomer, the U4 chip monomer, and the U8 chip monomer are consistent, the spacing between the upper limit grooves 1-17 of the inner ring arc is consistent, the width of the limit grooves 1-17 is slightly larger than the width of the buckle 2-3, and the connection structures of the two sides of the chip monomer and the other chip monomers are also consistent, that is, the U2 chip monomer, the U4 chip monomer, and the U8 chip monomer can be connected in pairs to form a complete disc-shaped chip body. The combined microfluidic chip of the present invention can be spliced ​​by a single U2 chip monomer, can be spliced ​​by a single U4 chip monomer, can be spliced ​​by a single U8 chip monomer, can be spliced ​​by a U2 chip monomer and a U4 chip monomer, or by a U2 chip monomer and a U8 chip monomer, by a U4 chip monomer and a U8 chip monomer, or by a U2 chip monomer, a U4 chip monomer, and a U8 chip monomer. As long as the total number of reaction pools 1-11 on all chip monomers is 32, they can be spliced ​​into a complete disc-shaped chip body, and parallel analysis of multiple samples and multiple indicators can be achieved.

[0040] There is a center hole in the center of the assembled disc-shaped chip body, which is formed by the inner ring arcs of all chip monomers in the disc-shaped chip body, and the 16 limiting grooves 1-17 on the circumference of the center hole are evenly distributed. Press the buckle 2-3 to make it close to the support column 2-2, and put the center hole of the disc-shaped chip body on the support column 2-2, so that the four buckles 2-3 extend from the four limiting grooves 1-17 around the center hole. After releasing the buckle 2-3, the buckle 2-3 is reset; the inner walls 2-9 of the four buckles respectively contact the bottoms of the four limiting grooves 1-17, limiting the radial movement of the disc-shaped chip body; the two sides of the buckle 2-3 prevent the groove wall of the groove 1-17 from moving left and right, limiting the circumferential movement of the disc-shaped chip body; the disc-shaped chip body is limited between the hook surface 2-10 of the buckle 2-3 and the support platform 2-1, limiting the axial movement of the disc-shaped chip body. There are also four inclined surfaces 1-18 of the limiting grooves 1-17 that respectively abut against the guide surfaces 2-5 of the four guide platforms 2-4, so that the disc-shaped chip body as a whole gradually tilts downward from the inner ring to the outer ring, so that the height of the reaction pool is lower than the height of the liquid storage cylinder, which is conducive to the sample liquid flowing from the liquid storage cylinder into the reaction pool during centrifugation, and can also further compress the disc-shaped chip body to limit the axial movement of the disc-shaped chip body.

[0041] The above are only preferred embodiments of the present invention and are not intended to limit the scope of implementation of the present invention. Without departing from the spirit and scope of the present invention, modifications or equivalent substitutions to the present invention, such as making some changes unrelated to the functional area on the fan-ring-shaped chip monomer, etc., should all be covered by the protection scope of the claims of the present invention.

Claims

1. A combined microfluidic chip, comprising a disc-shaped chip body and an adapter (2), wherein the adapter (2) is used to fix the disc-shaped chip body on a detection instrument, characterized in that: The disc-shaped chip body is composed of a plurality of sectors, each sector being provided with a chip monomer (1), each chip monomer (1) being provided with a fixing structure (1-1) cooperating with an adapter (2), a connection structure cooperating with chip monomers of other sectors, and a functional area for reaction detection, and both the front and back sides of the chip monomer are provided with a packaging layer in the functional area; The matching connection structure between chip monomers comprises a first connection step surface (1-12) arranged on the front side of the chip monomer (1) and a second connection step surface (1-14) arranged on the back side of the chip monomer (1); the first connection step surface (1-12) and the second connection step surface (1-14) between the chip monomers are correspondingly matched and connected; At least one connecting column (1-13) is provided on the first connecting step surface (1-12), at least one connecting hole (1-15) is provided on the second connecting step surface (1-14), and the connecting columns (1-13) and the connecting holes (1-15) between the chip monomers are matched and connected in a one-to-one correspondence; The chip monomer (1) is in the shape of a fan ring, and a plurality of chip monomers are connected to form a disc-shaped chip body, and the inner ring arcs of all chip monomers are spliced ​​to form the center hole of the disc-shaped chip body; the adapter (2) comprises a support platform (2-1) for supporting the disc-shaped chip body, a support column (2-2) for limiting the radial movement of the disc-shaped chip body, and at least one buckle (2-3) for limiting the axial movement of the disc-shaped chip body is arranged on the support platform (2-1); the center hole of the disc-shaped chip body is sleeved on the support column (2-2), the buckle (2-3) extends from the center hole, and the disc-shaped chip body is limited between the hook surface (2-10) of the buckle (2-3) and the support platform (2-1); At least one limiting groove (1-17) is provided on the inner circular arc of the chip monomer (1), and the buckle (2-3) extends from the central hole and is limited in the corresponding limiting groove (1-17); An inclined surface (1-18) is provided between the groove bottom of the limiting groove (1-17) and the back surface of the chip monomer (1); a plurality of guide platforms (2-4) are also provided on the support platform (2-1); a guide surface (2-5) inclined downward from the inside to the outside is provided on the top of the guide platform (2-4); a plurality of limiting grooves (1-17) are correspondingly connected to the buckles (2-3); and the inclined surfaces (1-18) of a plurality of limiting grooves (1-17) are in contact with the guide surfaces (2-5).

2. The combined microfluidic chip according to claim 1, characterized in that: The functional area comprises a liquid storage area (1-2), a microchannel area (1-7), and a reaction area (1-10); the liquid storage area (1-2) is provided with a liquid storage cylinder (1-3) and a sample addition hole (1-5) connected to the liquid storage cylinder (1-3), an exhaust hole (1-6), and a plurality of reserved grooves (1-4); the microchannel area (1-7) is provided with a plurality of microfluidic channels (1-8) connected to the reserved grooves (1-4) in a one-to-one correspondence; and the reaction area (1-10) is provided with a plurality of reaction pools (1-11) connected to the microfluidic channels (1-8) in a one-to-one correspondence.

3. The combined microfluidic chip according to claim 2, characterized in that: A ball valve (1-9) is arranged in each micro-flow channel (1-8).

4. The combined microfluidic chip according to claim 2, characterized in that: The number of reaction pools (1-11) on the disc-shaped chip body, the number of corresponding reserved grooves (1-4), and the number of microfluidic channels (1-8) are all 32.

5. The combined microfluidic chip according to claim 4, characterized in that: The number of reaction pools (1-11) on the chip monomer (1) and the number of corresponding reserved slots (1-4) and the number of microfluidic channels (1-8) are all K times 2, and the angle of the sector center angle θ of the sector where the chip monomer (1) is located is K times 22.5, K is an integer and 1≤K≤16.

6. The combined microfluidic chip according to claim 4, characterized in that: The number of reaction pools (1-11) on the chip monomer (1) and the number of corresponding reserved slots (1-4) and the number of microfluidic channels (1-8) are all M times 1, and the angle of the sector center angle θ of the sector where the chip monomer (1) is located is M times 11.25, where M is an integer and 1≤M≤32.

7. The combined microfluidic chip according to claim 2, characterized in that: The packaging layer on the back of the chip monomer is a transparent optical film, and the transparent optical film is used to seal the liquid storage cylinder (1-3), the reserved groove (1-4), the microfluidic channel (1-8) and the reaction pool (1-11).

8. The combined microfluidic chip according to claim 2, characterized in that: The packaging layer on the front side of the chip unit is used to seal the sample loading hole (1-5) and the exhaust hole (1-6).

9. The combined microfluidic chip according to claim 1, characterized in that: The buckle (2-3) and the support platform (2-1) are elastically connected.

10. The combined microfluidic chip according to claim 1, characterized in that: The adapter (2) is also provided with an adapting hole (2-6) for being installed in coordination with the detection instrument, and a positioning edge (2-7) is provided on the inner wall of the adapting hole (2-6).

11. The combined microfluidic chip according to claim 1, characterized in that: The adapter (2) is also provided with an adapting hole (2-6) for being installed in coordination with the detection instrument, and a positioning groove (2-8) is provided on the inner wall of the adapting hole (2-6).

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