A single cell library preparation system

Abstract

The present invention discloses a single-cell library preparation system, including a machine base, a transfer mechanism and an extrusion mechanism, wherein a working area is formed on the machine base; the transfer mechanism includes an inlet and outlet bin movably mounted on the machine base in a horizontal direction, and the inlet and outlet bin is used for installing a microfluidic chip; the extrusion mechanism includes a multi-branch plate disposed above the working area and movably arranged in an up-down direction, and used to connect with the microfluidic chip at the working area when the multi-branch plate moves downward to the working area, so as to drive the raw materials of the microfluidic chip to flow from each raw material slot through each flow channel in sequence and then be formed and flow into the forming slot. In the technical solution of the present invention, the microfluid is transferred by the transfer mechanism, and the extrusion mechanism drives the raw materials in the multiple raw material slots of the microfluidic chip to enter the flow channel of the microfluidic chip to be formed and flow into the forming slot of the microfluidic chip, thereby realizing the forming of a single single-cell sample and the preparation of a single-cell library.

Description

Technical Field

[0001] The present invention relates to the field of single cell library preparation, in particular to a single cell library preparation system. Background Art

[0002] In recent years, with the continuous advancement of sequencing technology, high-depth single-cell sequencing has developed towards higher-throughput applications. In the entire single-cell sequencing process, in addition to the base reading itself, sample cDNA synthesis and library preparation are another important cost component. The cDNA of each cell is labeled and the library is usually added through the independent sealing of thousands or tens of thousands of cells, reverse transcription, adding label sequences, library construction and other processes using a single-cell library preparation system. In other words, a single-cell library preparation system is now needed to realize the formation of a single single-cell sample and the preparation of a single-cell library. Summary of the invention

[0003] The main purpose of the present invention is to propose a single-cell library preparation system, aiming to provide a system to achieve single-cell sample formation and single-cell library preparation.

[0004] To achieve the above object, the present invention provides a single cell library preparation system, comprising:

[0005] A machine base is formed with a working area, and the machine base has a front-rear direction in the horizontal direction;

[0006] The transfer mechanism comprises an inlet and outlet bin movably mounted on the machine base in a front-to-back direction, the inlet and outlet bin is used for installing the microfluidic chip and can be moved in a front-to-back direction to the working area under the driving of an external force; and

[0007] The extrusion mechanism includes a multi-branched plate which is arranged above the working area and can be movably arranged in the up-down direction. When the multi-branched plate moves downward to the working area, it is used to connect with the microfluidic chip at the working area to drive the raw materials of the microfluidic chip to flow from each raw material tank through each flow channel in sequence, and then be formed and flow into the forming tank.

[0008] Optionally, the transfer mechanism further includes:

[0009] a first electric machine having a first output shaft; and

[0010] The transfer transmission assembly is used for transmission connection between the first output shaft and the in-and-out bin.

[0011] Optionally, the base also has left and right directions in the horizontal direction;

[0012] The first output shaft is arranged to extend in the left-right direction;

[0013] The transfer transmission assembly includes a gear and a rack that mesh with each other. The gear is installed on the first output shaft, and the rack is arranged at the bottom of the inlet and outlet bin, and the rack is extended in the front-rear direction.

[0014] Optionally, the transfer mechanism further includes a slide groove provided on one of the machine base and the entry and exit bin, and a corresponding slide bar provided on the other one, wherein the slide bar extends in the front-rear direction and is slidably installed in the slide groove.

[0015] Optionally, the extrusion mechanism further comprises an air pump mounted on the base;

[0016] An air passage is formed in the multi-branch plate, and an air inlet and a plurality of air outlets are respectively arranged on two sides of the multi-branch plate that are opposite to each other in an upward direction.

[0017] Wherein, the air inlet is connected to the air passage and the outlet of the air pump;

[0018] The plurality of gas outlets are used to respectively connect the gas passage and the plurality of raw material tanks of the microfluidic chip in the working area when the multi-manifold moves downward to the working area.

[0019] Optionally, the extrusion mechanism further includes:

[0020] a second electric machine having a second output shaft; and

[0021] An extrusion transmission assembly is drivingly connected between the second output shaft and the multi-branch plate.

[0022] Optionally, the second output shaft is arranged to extend in the front-rear direction;

[0023] The extrusion transmission assembly includes a cam mechanism, and the cam mechanism includes:

[0024] a cam mounted on the second output shaft; and

[0025] The push rod is extended in the up-down direction and has a first end and a second end opposite to each other in the up-down and upward direction. The first end abuts against the peripheral edge of the cam. The push rod can move in the up-down direction, and in its downward moving stroke, the second end is used to abut against the top of the multi-branch plate to push the multi-branch plate downward.

[0026] Optionally, the transfer mechanism includes a first motor drivingly connected to the in-and-out bin;

[0027] The extrusion mechanism includes an air pump;

[0028] The single cell library preparation system also includes:

[0029] an integrated circuit board, mounted on the top of the multi-branch board, for electrically connecting the first motor, the second motor and the air pump; and

[0030] A connecting plate, arranged on the top of the multi-branch plate and above the integrated circuit board;

[0031] Wherein, during the downward movement of the push rod, the second end is used to abut against the top of the connecting plate.

[0032] Optionally, the single-cell library preparation system further comprises a noise reduction structure provided on the machine base, and the noise reduction structure is arranged corresponding to the extrusion mechanism.

[0033] Optionally, the extrusion mechanism comprises an air pump mounted on the base;

[0034] The noise reduction structure includes a vibration damping member, the vibration damping member has a fixed section and a mounting section which are arranged opposite to each other in the front-rear direction, the fixed section is arranged on the machine base, and the mounting section is suspended outside the machine base;

[0035] Wherein, the air pump is installed on the installation section.

[0036] In the technical solution of the present invention, the single-cell library preparation system mainly includes a transfer mechanism and an extrusion mechanism, which act together on a microfluidic chip. It should be noted that the microfluidic chip includes a carrier plate, on which a first side surface and a second side surface are formed opposite to each other, a plurality of raw material grooves and a molding groove are sequentially formed on the first side surface, and a flow channel is formed on the second side surface, the flow channel is connected to the plurality of raw material grooves and the molding groove, and different raw materials are placed in the plurality of raw material grooves respectively. After the single-cell library preparation system is turned on, the microfluidic chip is placed in a feed bin of the transfer mechanism, and the microfluidic chip is transferred to the working area by the feed bin, the multi-plate of the extrusion mechanism moves downward, and is connected to the microfluidic chip assembly at the working area, driving each raw material in the plurality of raw material grooves to enter the flow channel to be formed into a single single-cell sample, and then flows into the molding groove, the operator counts and builds a library for the single single-cell samples in the molding groove, and the single-cell sample molding and single-cell library preparation are realized through the single-cell library preparation system. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0038] Figure 1A schematic diagram of the three-dimensional structure of an embodiment of a single cell library preparation system provided by the present invention;

[0039] Figure 2 for Figure 1 Schematic diagram of the three-dimensional structure of the single-cell library preparation system from another perspective;

[0040] Figure 3 for Figure 2 An enlarged schematic diagram of a local A in FIG.

[0041] Figure 4 for Figure 1 Front view schematic diagram of the single-cell library preparation system.

[0042] Description of the accompanying drawings of the embodiments provided by the present invention:

[0043] Label name Label name 100 Cell library preparation system 31 Multi-plate 1 Machine base 32 air pump 2 Transfer agency 33 Second motor 21 In and out of warehouse 34 Extrusion drive components 22 First Motor 341 Cam 23 Transfer drive components 342 mandrel 231 gear 4 Integrated Circuit Board 232 rack 5 Connecting plate 24 Chute 6 Noise reduction structure 25 Slider 61 Vibration Damping Parts 3 Extrusion mechanism

[0044] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0047] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0048] In recent years, with the continuous advancement of sequencing technology, high-depth single-cell sequencing has developed towards higher-throughput applications. In the entire single-cell sequencing process, in addition to the base reading itself, sample cDNA synthesis and library preparation are another important cost component. The cDNA of each cell is labeled and the library is usually added through the independent sealing of thousands or tens of thousands of cells, reverse transcription, adding label sequences, library construction and other processes using a single-cell library preparation system. In other words, a single-cell library preparation system is now needed to achieve single-cell sample formation and single-cell library preparation.

[0049] In view of this, the present invention provides a single cell library preparation system. Figures 1 to 4 This is an embodiment of the single cell library preparation system provided by the present invention.

[0050] See also Figure 1 The cell library preparation system 100 includes a base 1, a transfer mechanism 2 and an extrusion mechanism 3, wherein a working area is formed on the base 1, and the base 1 has a front-to-back direction in the horizontal direction; the transfer mechanism 2 includes an inlet and outlet bin 21 movably installed on the base 1 along the front-to-back direction, the inlet and outlet bin 21 is used for the installation of the microfluidic chip, and can be moved to the working area along the front-to-back direction under the drive of an external force; the extrusion mechanism 3 includes a multi-branched plate 31 arranged above the working area and movably arranged along the up-down direction, and is used to be connected with the microfluidic chip at the working area when the multi-branched plate 31 moves downward to the working area, so as to drive the raw materials of the microfluidic chip to flow from each raw material tank through each flow channel in sequence and then be formed and flow into the forming tank.

[0051] In the technical solution of the present invention, the single-cell library preparation system 100 mainly includes a transfer mechanism 2 and an extrusion mechanism 3, which act together on a microfluidic chip. It should be noted that the microfluidic chip includes a carrier plate, on which a first side surface and a second side surface are formed opposite to each other, a plurality of raw material grooves and a molding groove are sequentially formed on the first side surface, and a flow channel is formed on the second side surface, the flow channel is connected to the plurality of raw material grooves and the molding groove, and different raw materials are placed in the plurality of raw material grooves, respectively. After 00 is turned on, the microfluidic chip is placed in the inlet and outlet bin 21 of the transfer mechanism 2, and the microfluidic chip is transferred to the working area by the inlet and outlet bin 21. The multi-plate 31 of the extrusion mechanism 3 moves down and is connected with the microfluidic chip in the working area, driving each raw material in the multiple raw material tanks to enter the flow channel to be formed into a single single-cell sample, and then flow into the forming tank. The operator counts and builds a library for the single single-cell samples in the forming tank, and realizes single-cell sample forming and single-cell library preparation through the single-cell library preparation system 100.

[0052] It should be noted that the single-cell library preparation system 100 is also provided with a plurality of enclosures, which together with the base 1 form a sealed cavity, and the moving mechanism 2 and the extrusion mechanism 3 are arranged in the sealed cavity, so that the preparation environment is better, thereby improving the accuracy of single-cell library preparation.

[0053] Specifically, the transfer mechanism 2 also includes a first motor 22 and a transfer transmission assembly 23, the first motor 22 has a first output shaft; the transfer transmission assembly 23 is used to transmit and connect the first output shaft with the entry and exit warehouse 21, and the entry and exit warehouse 21 transports the microfluidic chip through the joint action of the transfer transmission assembly 23 and the first motor 22.

[0054] The present invention does not limit the specific installation method of the first output shaft and the specific form of the transfer transmission assembly 23. It can be that the first output shaft is extended in the front-to-back direction; the transfer transmission assembly 23 includes a ball screw, and the ball screw includes a screw and a nut with threaded matching. The screw is fixedly connected to the first output shaft and can rotate coaxially with the first output shaft. The peripheral side surface of the nut is fixedly connected to the bottom surface of the inlet and outlet bin 21; when the first motor 22 is started, the first output shaft rotates around the axis in the front-to-back direction, and at the same time drives the screw to rotate around the axis in the front-to-back direction. The nut can make a linear motion on the screw in the front-to-back direction, thereby driving the inlet and outlet bin 21 to move in the front-to-back direction to realize the transfer of the microfluidic chip; specifically, in this embodiment (please refer to Figure 2 and Figure 3), the base 1 also has a left and right direction in the horizontal direction, and the first output shaft is extended along the left and right direction; the transfer transmission assembly 23 includes a gear 231 and a rack 232 that are meshed with each other, the gear 231 is sleeved outside the first output shaft, and can rotate coaxially with the first output shaft, the rack 232 is arranged at the bottom of the entry and exit bin 21, and the rack 232 is extended along the front and back direction; when the first motor 22 is started, the first output shaft rotates around the left and right axis, and at the same time drives the gear 231 to rotate around the left and right axis, and the rack 232 meshing with the gear 231 can make a linear motion in the front and back and upward directions, thereby driving the entry and exit bin 21 to move in the front and back directions, thereby realizing the transfer of the microfluidic chip.

[0055] In order to facilitate the activities of entering and exiting the warehouse 21, further reference is made to Figure 3 The transfer mechanism 2 also includes a slide groove 24 provided on one of the base 1 and the in-and-out bin 21, and a corresponding slide bar 25 provided on the other one. The slide bar 25 is extended in the front-to-back direction and is slidably installed in the slide groove 24. Through the sliding cooperation between the slide groove 24 and the slide bar 25, the movement of the in-and-out bin 21 is smoother, thereby avoiding unnecessary vibration of the in-and-out bin 21 when transferring the microfluidic chip, thereby eliminating the risk of raw materials in the raw material tank overflowing due to vibration.

[0056] The present invention does not limit the specific setting positions of the slide groove 24 and the slide bar 25. The slide groove 24 can be set on the entry and exit bin 21, and correspondingly, the slide bar 25 can be set on the base 1. Specifically, in this embodiment, the slide groove 24 is set on the base 1, and the slide bar 25 is correspondingly set on the entry and exit bin 21.

[0057] It should be noted that in order to further stabilize the installation of the microfluidic chip, the microfluidic chip is provided with a mounting structure, and the mounting structure includes a base and a fixing structure. The upper end surface of the base is provided with a mounting groove, and the mounting groove is used for placing the microfluidic chip; the fixing structure is used to press the microfluidic chip on the bottom wall of the mounting groove, and a limiting portion is provided at the bottom of the base. Correspondingly, a mounting portion is also provided on the entry and exit bin 21, and the limiting portion cooperates with the mounting portion to fix the base to the entry and exit bin 21, thereby improving the stability of the microfluidic chip, thereby avoiding unnecessary vibration of the entry and exit bin 21 when transferring the microfluidic chip, and eliminating the risk of raw materials in the raw material tank overflowing due to vibration.

[0058] Specifically, the present invention does not limit the specific forms of the limiting portion and the mounting portion. The limiting portion may be a pin column, and correspondingly, the mounting portion may be a pin hole. The pin column and the pin hole are limitedly matched to fix the base to the entry and exit bin 21; the limiting portion and the mounting portion are respectively provided with two magnetic suction portions, and the two magnetic suction portions are mutually attracted to fix the base to the entry and exit bin 21. In this embodiment, the limiting portion is provided as an elastic clamping column, and correspondingly, the mounting portion is a clamping hole opened on the bottom plate of the entry and exit bin 21. The elastic clamping column and the clamping hole are mutually clamped to fix the base to the entry and exit bin 21, thereby making the installation of the microfluidic chip more stable.

[0059] In the present invention, in order to ensure the stability of the transfer of the in-and-out warehouse 21, the in-and-out warehouse 21 has a deceleration position and a stop position in its active stroke, and the deceleration position is before the stop position. In the process of the in-and-out warehouse 21 transferring the microfluidic chip into the working area, when the front end of the in-and-out warehouse 21 moves to the deceleration position, the movement of the in-and-out warehouse 21 begins to decelerate, and the in-and-out warehouse 21 continues to move. When the rear end of the in-and-out warehouse 21 reaches the stop position, the in-and-out warehouse 21 transfers the microfluidic chip completely into the working area, and the in-and-out warehouse 21 stops moving. In the process of the in-and-out warehouse 21 exiting the working area, when the front end of the in-and-out warehouse 21 When moving to the deceleration position, the movement of the in-and-out warehouse 21 begins to decelerate, and the in-and-out warehouse 21 continues to move. When the front end of the in-and-out warehouse 21 reaches the stop position, the in-and-out warehouse 21 completely exits the working area, and the in-and-out warehouse 21 stops moving. Correspondingly, in order to ensure that the moving position of the in-and-out warehouse 21 is more accurate, the transfer mechanism 2 also includes a first detector, and the first detector includes a first position sensor and a second position sensor. The first position sensor and the second position sensor are respectively arranged corresponding to the deceleration position and the working position, and are used to be triggered respectively when the in-and-out warehouse 21 moves to the deceleration position and the working position, so as to detect the moving position of the in-and-out warehouse 21.

[0060] A limiting structure is also provided in the transfer mechanism 2, and the limiting structure includes two limiting plates provided on the machine base 1, and the two limiting plates are respectively provided corresponding to the deceleration position and the stop position to limit the movement of the in-and-out bin 21 when the detector fails.

[0061] See also Figure 1 and Figure 4In the present invention, the extrusion mechanism 3 includes an air pump 32 installed on the machine base 1; an air passage is formed in the multi-manifold 31, and the multi-manifold 31 is respectively provided with an air inlet and a plurality of air outlets on two sides opposite to each other upward and downward; wherein the air inlet connects the air passage and the outlet of the air pump 32; the plurality of air outlets are used to connect the air passage and the plurality of raw material tanks of the microfluidic chip in the working area when the multi-manifold 31 moves downward to the working area, and the air pump 32 inflates the plurality of raw material tanks and the molding tank through the multi-manifold 31, and uses air pressure to drive the raw materials in the plurality of raw material tanks into the flow channel to be formed into a single single cell sample, and then flow into the molding tank to realize the molding of a single single cell sample.

[0062] Specifically, the extrusion mechanism 3 also includes a second motor 33 and an extrusion transmission assembly 34, the second motor 33 has a second output shaft; the extrusion transmission assembly 34 transmission connects the second output shaft and the multi-branch plate 31, and the multi-branch plate 31 is moved up and down and upward through the joint action of the extrusion transmission assembly 34 and the second motor 33, thereby enabling the extrusion mechanism 3 to complete the driving operation.

[0063] It should be noted that the present invention does not limit the specific installation method of the second output shaft and the specific form of the extrusion transmission assembly 34. It can be that the top of the multi-branch plate 31 is provided with a mounting groove, the second output shaft is extended in the up-down direction, and the extrusion transmission assembly 34 can include a ball screw, and the ball screw includes a screw and a nut with threaded matching. The screw is fixedly connected to the second output shaft at one end up and down and can rotate coaxially with the second output shaft. The other end of the screw is movably arranged in the mounting groove, and the lower end face of the nut is fixedly connected to the top of the multi-branch plate 31; when the second motor 33 is started, the second output shaft rotates around the up-down axis, and at the same time drives the screw to rotate around the up-down axis, and the nut can make a linear motion on the screw in the up-down direction, that is, It drives the multi-branch plate 31 to move in the up-down direction, realizes the up-down and upward movement of the multi-branch plate 31, and then enables the extrusion mechanism 3 to complete the driving operation; the extrusion transmission assembly 34 can also be a gear rack transmission assembly, and the gear rack transmission assembly includes a gear and a rack that mesh with each other. The gear is arranged outside the second output shaft and can rotate coaxially with the second output shaft. The rack is arranged on one side of the multi-branch plate 31 facing the gear, and the rack is extended in the up-down direction. When the second motor 33 is started, the second output shaft rotates around the up-down axis, and at the same time drives the gear to rotate around the up-down axis. The rack meshing with the gear can make a linear motion in the up-down and upward direction, which drives the multi-branch plate 31 to move in the up-down direction, and then enables the extrusion mechanism 3 to complete the driving operation.

[0064] Specifically, in this embodiment (please refer to Figure 4 ), the second output shaft is extended in the front-to-back direction; the extrusion transmission assembly 34 includes a cam mechanism, the cam mechanism includes a cam 341 and a push rod 342, the cam 341 is installed on the second output shaft; the push rod 342 is extended in the up-down direction, and has a first end and a second end opposite to each other in the up-down direction, the first end abuts against the periphery of the cam 341, the push rod 342 can move in the up-down direction, and in its downward moving stroke, the second end is used to abut against the top of the multi-branch plate 31 to push the multi-branch plate 31 downward, when the second motor 33 is started, the second output shaft rotates around the axis in the front-to-back direction, and at the same time drives the cam 341 to rotate around the axis in the front-to-back direction, because a convex portion is provided on the periphery of the cam 341, when the cam 341 rotates until the periphery of the convex portion abuts against the first end, the convex portion pushes the push rod 342 downward, and also pushes the multi-branch plate 31 downward, so that the extrusion mechanism 3 completes the driving operation.

[0065] More specifically, in order to cooperate with the operation of the cam 341, the extrusion transmission assembly 34 is also provided with an elastic reset member, which is arranged between the multi-branch plate 31 and the machine base 1, and the telescopic direction of the elastic reset member is arranged along the up and down direction; since a convex portion is arranged on the periphery of the cam 341, when the cam 341 rotates until the periphery of the convex portion abuts against the first end, the convex portion pushes the push rod 342 to move downward, thereby pushing the multi-branch plate 31 downward; when the cam 341 rotates until the edge of the convex portion leaves the first end, the elastic reset member drives the multi-branch plate 31 to move upward, so that the periphery of the cam 341 is always abutted against the first end, and the multi-branch plate 31 can also be moved along the up and down direction.

[0066] In this embodiment, the elastic return member is configured as a spring, and the spring is provided at the multi-branch plate 31 and the base 1, so that when the cam 341 rotates until the edge of the protrusion leaves the first end, the elastic return member drives the multi-branch plate 31 to move upward.

[0067] In the present invention, the extrusion mechanism 3 is further provided with a second detector, and the second detector is used to detect the displacement of the multi-branch plate 31 in the up and down directions to ensure the accuracy of the displacement of the multi-branch plate 31.

[0068] Specifically, the present invention does not limit the specific form of the second detector. The second detector can be a distance sensor. The distance sensor is set corresponding to the convex part of the cam 341 and is set above the cam 341. During the rotation of the cam 341, the displacement of the multi-branch plate 31 is detected by measuring the position of the convex part. In this embodiment, the second detector is set as a coding disk, and the coding disk is set on the second output shaft to detect the displacement of the multi-branch plate 31. It should be noted that the coding disk refers to a digital encoder for measuring angular displacement. It has the advantages of strong resolution, high measurement accuracy and reliable operation. It is a most commonly used displacement sensor for measuring the angular position of the shaft. The coding disk is placed on the second output shaft, and the displacement of the multi-branch plate 31 is measured by detecting the rotation angle of the second output shaft, making the detection more convenient.

[0069] In the present invention, the single-cell library preparation system 100 also includes an integrated circuit board 4 and a connecting board 5. The integrated circuit board 4 is installed on the top of the multi-manifold plate 31 to electrically connect the first motor 22, the second motor 33 and the air pump 32; the connecting board 5 is arranged on the top of the multi-manifold plate 31 and is above the integrated circuit board 4; wherein, during the downward movement of the push rod 342, the second end is used to abut against the top of the connecting board 5, and the integrated circuit board 4 is arranged on the top of the multi-manifold plate 31, so that the structural setting is more reasonable and the installation space is saved.

[0070] In order to prevent the integrated circuit board 4 from being damaged due to direct contact between the extrusion transmission assembly 34 and the integrated circuit board 4, the connecting plate 5 is also arranged above the integrated circuit board 4, and the push rod 342 is connected to the connecting plate 5, which not only enables the multi-branch plate 31 to move in the up and down directions, but also prevents the integrated circuit board 4 from being damaged due to direct contact between the push rod 342 and the integrated circuit board 4.

[0071] It should be noted that due to the processing size errors and assembly errors among the components, in order to ensure the sealing of the multi-branch plate 31, a support plate is also provided on the machine base 1, and the support plate is located above the connecting plate 5. The push rod 342 passes through the connecting plate 5 and can move up and down relative to the connecting plate 5. The extrusion mechanism 3 is also provided with a gap adjustment gasket, which is arranged at the upper end of the support plate and is correspondingly sleeved on the outer side of the push rod 342. The push rod 342 moves downward and adjusts the movable stroke of the multi-branch plate 31 in the up and down directions by abutting against the gap adjustment gasket, thereby improving the sealing performance of the multi-branch plate.

[0072] It should be noted that in the present invention, please refer to Figure 1 and Figure 2 The single-cell library preparation system 100 further includes a noise reduction structure 6 disposed on the machine base 1. The noise reduction structure 6 is disposed corresponding to the extrusion mechanism 3 to eliminate the noise generated by the extrusion mechanism 3 when it is working. Since the extrusion mechanism 3 provides a driving force when the single-cell library preparation system 100 is started, it is inevitable that vibration and noise will be caused, thereby affecting the normal operation of the single-cell library preparation system 100. The noise reduction structure 6 is disposed corresponding to the extrusion mechanism 3 to eliminate the noise generated by the extrusion mechanism 3 when it is working, which not only improves the working environment of the single-cell library preparation system 100, but also eliminates the influence of vibration and noise on single-cell library preparation.

[0073] Specifically, the noise reduction structure 6 includes a vibration damping member 61, and the vibration damping member 61 has a fixed section and a mounting section which are relatively arranged in the front-to-back direction, the fixed section is arranged on the machine base 1, and the mounting section is suspended outside the machine base 1; wherein, the air pump 32 is installed on the mounting section. Since the mounting section is suspended on the machine base 1, when the extrusion mechanism 3 is working, the mounting section will swing up and down, thereby absorbing the up and down vibration of the extrusion mechanism 3, and then eliminating the noise generated by the vibration.

[0074] More specifically, the vibration damper 61 is arranged in a plate shape, and the vibration damper 61 is bent and extended toward one side near the mounting section to form a stepped surface. The stepped surface and the mounting section together define a mounting groove. The air pump 32 is accommodated in the mounting groove, and the side wall of the air pump 32 is supported on the stepped surface. In this way, the side wall of the air pump 32 is supported by the stepped surface, so that the air pump 32 is more stable during operation.

[0075] Furthermore, the fixed section is extended along the surface of the machine base 1 at the location to form a fixed plate, and the fixed section is installed on the machine base 1 through the fixed plate. The fixed plate is in surface contact with the surface of the machine base 1 to prevent the vibration damping member 61 from exerting excessive pressure on the surface of the machine base 1 and damaging the surface of the machine base 1 when the installation section swings up and down.

[0076] In this embodiment, reinforcing ribs are provided between the fixing section and the fixing plate, so that the connection between the fixing section and the fixing plate is more secure, thereby improving the stability of the fixing section when it is installed on the machine base 1.

[0077] In addition, a fixing hole is provided on the upper end surface of the base 1, and a through hole is provided on the fixing plate corresponding to the fixing hole. The noise reduction structure 6 also includes a screw connection (not shown in the figure), and the screw connection threadedly connects the through hole and the fixing hole to fix the fixing plate and the base 1 to each other. The fixing plate is connected to the base 1 via the screw connection, and the connection is reliable and easy to disassemble.

[0078] In this embodiment, the noise reduction structure 6 also includes a damping sleeve, which is at least partially disposed in the through hole and sleeved on the outer periphery of the threaded connector. Such a configuration helps to eliminate the vibration of the fixing plate in the horizontal direction, thereby eliminating the vibration of the air pump 32 in the horizontal direction.

[0079] Specifically, the outer wall of the damping sleeve is recessed inward to form an annular clamping groove, and the two side walls of the annular clamping groove are respectively clamped with the circumferential sides of the two ports of the through hole, which helps to eliminate the up and down vibration of the fixed plate, thereby further eliminating the up and down vibration of the air pump 32.

[0080] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A single cell library preparation system, characterized in that: include: A machine base is formed with a working area, and the machine base has a front-rear direction in the horizontal direction; The transfer mechanism comprises an inlet and outlet bin movably mounted on the machine base in a front-to-back direction, the inlet and outlet bin is used for installing the microfluidic chip, and can be moved to the working area in a front-to-back direction under the driving of an external force; as well as, The extrusion mechanism comprises a multi-branched plate disposed above the working area and movable in the up-down direction, wherein the multi-branched plate is used to be connected with the microfluidic chip at the working area when it moves downward to the working area, so as to drive the raw materials of the microfluidic chip to flow from the raw material tanks through the flow channels in sequence, and then be formed and flow into the forming tank; The extrusion mechanism also includes an air pump mounted on the base; An air passage is formed in the multi-branch plate, and an air inlet and a plurality of air outlets are respectively arranged on two sides of the multi-branch plate that are opposite to each other in an upward direction. Wherein, the air inlet is connected to the air passage and the outlet of the air pump; The plurality of gas outlets are used to respectively connect the gas passage and the plurality of raw material tanks of the microfluidic chip in the working area when the multi-manifold moves downward to the working area.

2. The single cell library preparation system according to claim 1, characterized in that: The transfer mechanism also includes: a first electric machine having a first output shaft; and The transfer transmission assembly is used for transmission connection between the first output shaft and the in-and-out bin.

3. The single cell library preparation system according to claim 2, characterized in that: The base also has left and right directions in the horizontal direction; The first output shaft is arranged to extend in the left-right direction; The transfer transmission assembly includes a gear and a rack that mesh with each other. The gear is installed on the first output shaft, and the rack is arranged at the bottom of the inlet and outlet bin, and the rack is extended in the front-rear direction.

4. The single cell library preparation system according to claim 1, characterized in that: The transfer mechanism further includes a slide groove provided on one of the machine base and the in-and-out bin, and a slide bar provided on the other one, wherein the slide bar extends in the front-back direction and is slidably installed in the slide groove.

5. The single cell library preparation system according to claim 1, characterized in that: The extrusion mechanism also includes: a second electric machine having a second output shaft; and An extrusion transmission assembly is drivingly connected between the second output shaft and the multi-branch plate.

6. The single cell library preparation system according to claim 5, characterized in that: The second output shaft is arranged to extend in the front-rear direction; The extrusion transmission assembly includes a cam mechanism, and the cam mechanism includes: a cam mounted on the second output shaft; and The push rod is extended in the up-down direction and has a first end and a second end opposite to each other in the up-down and upward direction. The first end abuts against the peripheral edge of the cam. The push rod can move in the up-down direction, and in its downward moving stroke, the second end is used to abut against the top of the multi-branch plate to push the multi-branch plate downward.

7. The single cell library preparation system according to claim 6, characterized in that: The transfer mechanism includes a first motor driving and connecting the in-and-out bin; The extrusion mechanism includes an air pump; The single cell library preparation system also includes: an integrated circuit board, mounted on the top of the multi-branch board, for electrically connecting the first motor, the second motor and the air pump; and A connecting plate, arranged on the top of the multi-branch plate and above the integrated circuit board; Wherein, during the downward movement of the push rod, the second end is used to abut against the top of the connecting plate.

8. The single cell library preparation system according to claim 1, characterized in that: The single-cell library preparation system further includes a noise reduction structure disposed on the machine base, and the noise reduction structure is disposed corresponding to the extrusion mechanism.

9. The single cell library preparation system according to claim 8, characterized in that: The extrusion mechanism includes an air pump mounted on the base; The noise reduction structure includes a vibration damping member, the vibration damping member has a fixed section and a mounting section which are arranged opposite to each other in the front-rear direction, the fixed section is arranged on the machine base, and the mounting section is suspended outside the machine base; Wherein, the air pump is installed on the installation section.

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