Rapeseed bee pollen facial mask, preparation method and application

Abstract

The invention discloses a rape pollen mask and a preparation method, and belongs to the technical field of mask preparation. The mask ingredients include glycerin, sodium hyaluronate, carbomer, triethanolamine, rape bee pollen extract, 1,2-propylene glycol, vitamin B5, methyl paraben, hydrogenated castor oil and deionized water, and the preparation method of the rape pollen mask includes the following steps: 1) preparing phase A: weighing the corresponding mass of deionized water, adding glycerin, stirring until the water and glycerin are completely dissolved, adding sodium hyaluronate to completely dissolve it, adding a certain mass of carbomer, and stirring until uniform; 2) preparing phase B: weighing a certain amount of 1,2-propylene glycol, adding methyl paraben thereto, and then placing it in a 60°C water bath and stirring until completely dissolved; 3) after mixing phase A and phase B evenly, hydrogenated castor oil, rape bee pollen flavonoid extract and vitamin B5 are added thereto one by one, and finally adjusted to a suitable pH with triethanolamine, and continuously stirred to eliminate bubbles.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic preparation, and more specifically, relates to a rapeseed bee pollen facial mask, a preparation method and application thereof. Background Art

[0002] Nowadays, there are more and more conceptualized facial mask products, such as whitening, moisturizing, and freckle removal masks. Judging from the sales volume, consumers are very fond of such conceptualized facial masks with obvious effects. However, when consumers use such products in large quantities, they also have some concerns about the performance of such products. Delaying skin aging has become one of the research focuses of cosmetics. The human skin, like other organs, gradually tends to age with increasing age. Aging makes the human body less beautiful, so the aging appearance will have a negative effect on the work and life of people who love beauty. With the improvement of living standards, there is also a greater market demand for anti-aging cosmetics. In particular, cosmetics developed with natural extracts as raw materials are more popular among people. However, it is more difficult to obtain facial masks with good anti-aging performance, moisturizing performance and comprehensive performance through natural extracts.

[0003] Rapeseed bee pollen is a fine granular substance that is collected by bees from the male reproductive cells of flowering plants and brought to the hive as bee food. Pollen is rich in nutrients, including proteins, lipids, vitamins, sugars and flavonoids, and is known as a micro-nutrient bank. Modern pharmacology has confirmed that pollen can prevent and treat a variety of diseases, and has the functions of regulating the nervous system, lowering blood lipids, improving the body's immunity, preventing and fighting cancer, and inhibiting prostate hyperplasia. In addition, bee pollen has obvious cosmetic effects, can prevent skin aging, and has the ability to scavenge free radicals.

[0004] After searching, it was found that applications for preparing facial masks with bee pollen have been disclosed in the prior art. For example, the Chinese patent application number is 201210219033.6, and the publication date is November 14, 2012, which discloses a pure natural bee pollen facial mask cream and a production method thereof. The weight percentage of its constituent raw materials is 33-38% of broken rapeseed pollen, 50-55% of rapeseed honey, 7-9% of fresh royal jelly, and 4-6% of pearl powder.

[0005] For example, the Chinese patent application number CN201810112693.1 and the publication date of 2018-05-29 discloses a facial mask containing anthocyanins and bee pollen and a preparation method thereof, wherein broken rose bee pollen, rose honey, anthocyanins, pearl powder, rose essential oil, antioxidants, hyaluronic acid, glycerin and deionized water are placed in ultraviolet light for sterilization and disinfection; broken rose bee pollen, anthocyanins and pearl powder are placed in cooled deionized water for soaking, and then rose honey, rose essential oil, antioxidants, hyaluronic acid and glycerin are added, heated and stirred in a water bath to obtain a mixture; the mixture is placed in a high-pressure homogenizer for full homogenization to obtain a facial mask. The method of this application uses rose bee pollen as male reproductive cells, which contains 22 amino acids, 14 vitamins and more than 30 trace elements as well as a large amount of active proteases, nucleic acids, flavonoids and other active substances. It also contains VE, superoxide dismutase (SOD), selenium and other ingredients that can regulate skin metabolism, promote the excretion of metabolic waste such as free radicals and lipofuscin, protect skin elasticity and delay aging; at the same time, the flavonoids in anthocyanin phenolic compounds have strong free radical scavenging and antioxidant capabilities, so the broken rose bee pollen and anthocyanins are directly mixed as facial mask raw materials for application. Although it has a certain beauty and skin care effect, the rich nutrients in the pollen have low availability. As consumers' requirements for facial masks increase, additional ingredients such as antioxidants are needed to meet consumers' requirements for facial masks.

[0006] Therefore, based on the defects of the prior art, there is an urgent need to invent a natural, pollution-free facial mask that has excellent moisturizing properties, anti-aging properties and comprehensive performance. Summary of the invention

[0007] 1. Problem to be solved

[0008] In view of the defect that the bee pollen masks in the prior art are directly added to the masks, which makes it difficult to effectively utilize the nutrients, the present invention extracts bee pollen and then uses rutin to control the quality of the flavonoid content therein, adds glycerin and sodium hyaluronate as moisturizers, carbomer as a gel matrix, triethanolamine, 1,2-propylene glycol, vitamin B 5 , methylparaben, hydrogenated castor oil and deionized water are used as other ingredients to prepare a natural and pollution-free facial mask with excellent moisturizing, anti-aging and comprehensive performance.

[0009] 2. Technical solution

[0010] In order to solve the above problems, the technical solution adopted by the present invention is as follows:

[0011] The invention provides a rape pollen facial mask, wherein ingredients of the facial mask include glycerin, sodium hyaluronate, carbomer, triethanolamine, rape bee pollen extract, 1,2-propylene glycol, vitamin B5, methyl paraben, hydrogenated castor oil and deionized water.

[0012] As a further improvement of the present invention, the flavonoids content in the rape bee pollen extract is 5-25%.

[0013] As a further improvement of the present invention, the mass fraction of glycerol in the facial mask is 8-16%, the mass fraction of carbomer is 0.3-0.7%, and the mass fraction of triethanolamine is 0.3-0.7%.

[0014] As a further improvement of the present invention, the mass fraction of glycerol in the facial mask is 12%, the mass fraction of carbomer is 0.4%, the mass fraction of triethanolamine is 0.5%, and the mass fraction of rapeseed bee pollen flavonoid extract is 15%.

[0015] As a further improvement of the present invention, the facial mask contains 68.25% deionized water, 12% glycerol, 0.05% sodium hyaluronate, 0.4% carbomer, 3% 1,2-propylene glycol, 0.2% methylparaben, 0.5% vitamin B5, 0.1% hydrogenated castor oil, 15% rapeseed bee pollen flavonoid extract and 0.5% triethanolamine.

[0016] The preparation method of the rapeseed pollen facial mask comprises the following steps:

[0017] 1) Prepare phase A: weigh a corresponding amount of deionized water, add glycerol, stir until the water and glycerol are completely dissolved, add sodium hyaluronate, dissolve it completely, add a certain amount of carbomer, and stir until uniform;

[0018] 2) Prepare phase B: weigh a certain amount of 1,2-propylene glycol, add methylparaben thereto, and then place in a 60°C water bath and stir until completely dissolved;

[0019] 3) After mixing phase A and phase B evenly, add hydrogenated castor oil, rapeseed bee pollen flavonoid extract and vitamin B5 one by one, and finally adjust the pH to a suitable value with triethanolamine, and keep stirring to eliminate bubbles.

[0020] As a further improvement of the present invention, the preparation of the flavonoid extract in rape bee pollen comprises the following steps:

[0021] a) extracting flavonoids from bee pollen using ultrasonic method to obtain a crude extract;

[0022] b) using a rutin standard to determine the flavonoid content in the crude extract to obtain a rape bee pollen flavonoid extract having a flavonoid content of 5 to 25%;

[0023] As a further improvement of the present invention, the preparation steps of the crude extract in step a) are as follows:

[0024] 1) Weigh a certain amount of rapeseed bee pollen, add 10 times the volume of 50% ethanol, and soak at room temperature for 2 hours;

[0025] 2) Ultrasonic extraction was performed for 20 min, and the extraction was repeated three times. The supernatant was centrifuged at 2000 r / min for 10 min, and the volume of the supernatant was fixed with 50% ethanol solution to obtain a crude extract.

[0026] Application of a rapeseed pollen facial mask, application of the rapeseed pollen facial mask in paper facial masks, using the rapeseed pollen facial mask to make a paper facial mask, and directly sticking the paper facial mask on the skin when using it.

[0027] 3. Beneficial effects

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The present invention has found that rape bee pollen is rich in flavonoids, which have strong biological effects of removing free radicals and anti-oxidation. Since bee pollen has a high content of flavonoids, the extraction method is simple, the materials are economical, the source of bee pollen is wide, and flavonoids have a strong ability to remove free radicals, it can protect the skin. The mask contains sodium hyaluronate, which can keep the skin moisturized, delicate, tender, and elastic, and has the effects of anti-wrinkle, freckle removal, beauty care, and restoration of skin physiological functions.

[0030] (2) The rapeseed pollen mask of the present invention is prepared by extracting flavonoids from rapeseed bee pollen and controlling the flavonoid content in the extract using a rutin standard, while simultaneously using glycerin and sodium hyaluronate as moisturizers, carbomer as a gel matrix, triethanolamine, 1,2-propylene glycol, and vitamin B 5 , methyl paraben, hydrogenated castor oil and deionized water to prepare a facial mask with excellent moisturizing and anti-aging properties, as well as high-quality properties such as viscosity, pH, fineness and uniformity.

[0031] (3) The present invention improves the production formula of the facial mask and adds some natural plant extracts. Studies have found that flavonoids have important physiological activities in organisms, mainly in anti-aging and anti-oxidation, and still have the effect of scavenging free radicals and anti-oxidation in vitro. By introducing natural plant extracts into the facial mask formula, it not only has the natural green characteristics, but also has the biological function of anti-oxidation and delaying skin aging, while also ensuring the quality of the facial mask product.

[0032] (4) The present invention has a simple structure, reasonable design and is easy to manufacture. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The standard curve of rutin concentration and absorbance. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with specific embodiments.

[0035] Example 1

[0036] The preparation process of the facial mask in this embodiment comprises the following steps:

[0037] A) Ultrasonic extraction of flavonoids from rapeseed bee pollen;

[0038] This experiment uses ultrasonic method to extract flavonoids from rape bee pollen. This method is easy to operate, suitable for extraction with various solvents, and has a short extraction time. It is also suitable for the extraction of heat-labile components. The specific method is as follows:

[0039] 1) Weigh a certain amount of rapeseed bee pollen, add 10 times the volume of 50% ethanol, and soak at room temperature for 2 hours;

[0040] 2) Ultrasonic extraction for 20 min, 3 times;

[0041] 3) Centrifuge at 2000 r / min for 10 min, dilute the supernatant to 100 mL with 50% ethanol solution to obtain a crude extract containing 0.037 g / mL rapeseed bee pollen, and refrigerate for later use.

[0042] B) Determination of flavonoids content in rapeseed bee pollen (drawing of rutin standard curve)

[0043] Accurately weigh 10 mg of rutin standard dried at 100°C to constant weight, dissolve it in 75% ethanol and dilute it to a 100 mL volumetric flask to obtain a 0.1 mg / mL rutin standard solution. Pipette 0, 1, 2, 3, 4, and 5 mL of the standard solution into 25 mL graduated test tubes, dilute to 5 mL with 75% ethanol solution, and then add 5% NaNO to each test tube. 2 0.3 mL of solution was added to each test tube 6 min later. 3 ) 3 0.3 mL of the solution was shaken well. After 6 minutes, 4 mL of 10% NaOH solution was added to each test tube and shaken well. The volume was made up to 10 mL with 75% ethanol solution. The solution was allowed to stand for 15 minutes and the absorbance at 510 nm was measured.

[0044] Rutin is a flavonoid component and a representative component of flavonoid components. Rutin is the flavonoid component with the highest content in bee pollen. Rutin is used as a standard curve to more effectively control the quality of the mask. Determination of flavonoid content in rapeseed bee pollen: The data obtained by rutin standard curve are shown in Table 1. The standard curve of rutin concentration and absorbance is shown in Figure 1 , the regression equation is: y = 0.2861x + 0.0036 (R 2 =0.9903). The corresponding figure is as follows Figure 1 shown.

[0045] Table 1 Standard solution concentration and absorbance

[0046]

[0047] Total flavonoids yield of rape bee pollen (g / g) = M 1 / M 2 *100%

[0048] Among them, M 1 is the mass of total flavonoids in the crude extract, M 2 is the mass of rape bee pollen raw material. The total flavonoid yield is calculated to be 0.93%. The flavonoid content per gram of crude extract is 93 mg (i.e., the flavonoid content per gram of crude extract is 93 mg).

[0049] B) Preparation of facial mask

[0050] 1) First, prepare phase A: weigh a corresponding amount of deionized water, add glycerin, stir until the water and glycerin are completely dissolved, add sodium hyaluronate, and use a high shear dispersing emulsifier to completely dissolve it; add a certain amount of carbomer, and use a high shear dispersing emulsifier to stir until uniform.

[0051] 2) Preparation of phase B: Weigh a certain amount of 1,2-propylene glycol, add methylparaben thereto, and then stir with a glass rod in a 60°C water bath until completely dissolved.

[0052] 3) After mixing phase A and phase B evenly, add hydrogenated castor oil (solubilizer), rapeseed bee pollen flavonoid extract and vitamin B 5 (Nutritional additives) are added thereto; finally, the pH value is adjusted to a suitable value with triethanolamine, and the mixture is stirred continuously to eliminate bubbles, and then the mixture is placed in an ultrasonic cleaner to remove the bubbles.

[0053] Table 2 Basic formula of rapeseed bee pollen mask

[0054]

[0055] The facial mask prepared in this embodiment has the following ingredients: 68.25% by mass of deionized water, 12% by mass of glycerol, 0.05% by mass of sodium hyaluronate, 0.4% by mass of carbomer, 3% by mass of 1,2-propylene glycol, 0.2% by mass of methylparaben, and 0.2% by mass of vitamin B12. 5 The mass fraction is 0.5%, the mass fraction of hydrogenated castor oil is 0.1%, the mass fraction of rapeseed bee pollen flavonoids extract is 15%, and the mass fraction of triethanolamine is 0.5%.

[0056] The rapeseed pollen facial mask is used to make a paper facial mask, and the paper facial mask is directly attached to the skin during use.

[0057] Example 2

[0058] This embodiment is basically the same as embodiment 1, except that: the ingredients of the prepared facial mask are as follows: the mass fraction of deionized water is 81.9%, the mass fraction of glycerol is 8%, the mass fraction of sodium hyaluronate is 0.7%, the mass fraction of carbomer is 0.3%, the mass fraction of 1,2-propylene glycol is 3%, the mass fraction of methylparaben is 0.2%, and the mass fraction of vitamin B 5 The mass fraction is 0.5%, the mass fraction of hydrogenated castor oil is 0.1%, the mass fraction of rapeseed bee pollen flavonoids extract is 5%, and the mass fraction of triethanolamine is 0.3%.

[0059] Example 3

[0060] This embodiment is basically the same as embodiment 1, except that: the facial mask prepared in this embodiment has the following ingredients: deionized water with a mass fraction of 53.5%, glycerol with a mass fraction of 16%, sodium hyaluronate with a mass fraction of 0.3%, carbomer with a mass fraction of 0.7%, 1,2-propylene glycol with a mass fraction of 3%, methylparaben with a mass fraction of 0.2%, vitamin B 5 The mass fraction of the raw material is 0.5%, the mass fraction of hydrogenated castor oil is 0.1%, the mass fraction of rapeseed bee pollen flavonoids extract is 25%, and the mass fraction of triethanolamine is 0.7%.

[0061] Comparative Example 1

[0062] The preparation steps of this embodiment are basically the same as those of Embodiment 1, except that rapeseed bee pollen is directly added in the step of preparing the facial mask, and the quality of all substances is the same as that of Embodiment 1.

[0063] 1) First, prepare phase A: weigh a corresponding amount of deionized water, add glycerin, stir until the water and glycerin are completely dissolved, add sodium hyaluronate, and use a high shear dispersing emulsifier to completely dissolve it; add a certain amount of carbomer, and use a high shear dispersing emulsifier to stir until uniform.

[0064] 2) Preparation of phase B: Weigh a certain amount of 1,2-propylene glycol, add methylparaben thereto, and then stir with a glass rod in a 60°C water bath until completely dissolved.

[0065] 3) After mixing phase A and phase B evenly, add hydrogenated castor oil (solubilizer), rapeseed bee pollen and vitamin B5 (nutritional additive) one by one; finally, adjust the pH to a suitable value with triethanolamine, stir continuously to eliminate bubbles, and then put it into an ultrasonic cleaner to remove bubbles.

[0066] Comparative Example 2

[0067] This embodiment is basically the same as Embodiment 1, except that the mass concentration of the triethanolamine is 0.2%.

[0068] Comparative Example 3

[0069] This embodiment is basically the same as Embodiment 1, except that the mass concentration of the triethanolamine is 0.6%.

[0070] Example 4

[0071] A) Moisture retention test:

[0072] Select a glass plate of appropriate size, stick a layer of medical breathable tape on it, and place it in the room overnight to make its quality stable. Weigh 0.5g of sample and evenly apply it on the tape on the glass plate, record the initial weight, place the glass plate in a dryer for hygroscopic chemicals, weigh and record the mass of each sample after 48 hours, compare the mass difference before and after placement, calculate the moisture retention rate W (%), and conduct 5 parallel experiments on Examples 1 to 3 and the comparative example.

[0073] W(%)=M 2 / M 1 ×100%

[0074] Where: M 1 M is the mass of the sample before placement; 2 The moisture retention rate is calculated based on the sample mass after placement.

[0075] Table 3 Moisturizing activity of facial mask products in different embodiments and comparative examples

[0076]

[0077] As can be seen from the above table, the product has a good moisturizing effect, and the moisturizing effects of the masks with different implementation ratios and comparison ratios are significantly different. Among them, the moisturizing effects of the masks with different bee pollen flavonoid extracts are significantly different, and compared with the direct use of bee pollen, it can be clearly seen that the moisturizing effect is significantly reduced. Therefore, the use of bee pollen flavonoid extracts has a stronger moisturizing effect than directly adding bee pollen.

[0078] B) Antioxidant activity test

[0079] The antioxidant activity of the facial mask products of each embodiment and comparative example was tested. Samples were taken in the order of sample retention time, and the facial mask combinations described in embodiments 1 to 3 and comparative examples were selected. The antioxidant activity was tested using the DPPH free radical scavenging method. The results are shown in Table 4.

[0080] DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) enzyme marker method was used for detection. 100ul of the facial mask liquid in Examples 1, 2, and 3 were added to 100ul of 1mmol / L DPPH free radical ethanol solution, shaken for 30 seconds, kept warm at 37°C for 20min, and the absorbance was measured at a wavelength of 517nm using an enzyme marker. The measurement was repeated 3 times. Calculate the free radical scavenging rate K (%) = [1-(Ai-Aj) / Ac] × 100%.

[0081] In the formula, Ai is the initial absorbance of the reaction; Aj is the absorbance after the reaction; Ac is the absorbance of the control test (only DPPH ethanol solution is added).

[0082] Table 4 Antioxidant activity of facial mask products of different embodiments and comparative examples at different retention times

[0083]

[0084] In vitro tests show that the product has good stability within 18 months. Comparison of each case shows that bee pollen flavonoid extract has good antioxidant effect. Within a certain range, the antioxidant performance of the mask product has a certain dose dependence on the flavonoid extract content. Comparison of each case shows that the free radical scavenging ability of 5% flavonoid extract is significantly weaker than that of 15% and 25% flavonoid extract. The antioxidant activity of the mask with flavonoid extract added is significantly stronger than that of the mask with bee pollen directly added. Therefore, flavonoid extract has a strong ability to scavenge free radicals in the mask, and has the ability to protect the skin and reduce skin aging.

[0085] Example 5

[0086] This example is a scoring test of the facial masks prepared in each embodiment and comparative example:

[0087] The specific scoring criteria are shown in Table 5. The higher the comprehensive score, the better the quality of the mask. Among them, viscosity: measured by NDJ-8S viscometer. pH value: take 5g of rapeseed bee pollen mask, add 50mL of deionized water, stir to mix evenly, put it in a constant temperature water bath of 85-90℃, let it boil slightly, cool to room temperature, and measure the pH of the suspension with a pH meter. The most suitable pH for the human body is 5.5.

[0088] Table 5 Comprehensive scoring criteria for rapeseed bee pollen mask

[0089]

[0090] Table 6 Comprehensive scores of facial mask products in different embodiments and comparative examples

[0091]

[0092] Example 6

[0093] This example adopts the preparation method and dosage of each component of Example 1 (only the mass fraction of rapeseed bee pollen flavonoid extract is different in the experiment). The comprehensive scores of rapeseed bee pollen flavonoid extracts with different mass fractions are shown in Table 7.

[0094] Table 7 Comprehensive scores of rapeseed bee pollen flavonoid extracts with different mass fractions

[0095]

[0096] Table 8 Comparison of free radical scavenging rate and moisturizing rate of rapeseed bee pollen flavonoid extracts with different mass fractions

[0097]

[0098] The results show that the free radical scavenging ability of the mask increases with the increase of the flavonoid extract content. When the flavonoid extract content is less than 15%, the free radical scavenging ability and moisturizing rate of the mask increase with the increase of the content, but when the content exceeds 15%, the effect of increasing the flavonoid extract content on the free radical scavenging of the mask is small, but the effect on the moisturizing rate of the mask is large, which decreases significantly with the increase of the content. In summary, when the flavonoid extract content is 15%, it not only ensures a high free radical scavenging ability but also ensures the moisturizing effect of the mask. The free radical scavenging ability of the material is limited, and the continuous improvement of the flavonoid extract has limited impact on the function of the entire product, but the utilization rate of resources is decreasing. Therefore, the flavonoid extract content is at a concentration of 15%, which ensures the quality of the product while improving the utilization rate of resources.

Claims

1. A method for preparing a rapeseed pollen facial mask, characterized in that: The following steps are involved: 1) Prepare phase A: weigh the corresponding mass of deionized water, add glycerol, stir until the water and glycerol are completely dissolved, add sodium hyaluronate, dissolve it completely, add a certain mass of carbomer, and stir until uniform; 2) Prepare phase B: Weigh a certain amount of 1,2-propylene glycol, add methylparaben to it, and then place it in a 60°C water bath and stir until it is completely dissolved; 3) After mixing phase A and phase B evenly, add hydrogenated castor oil, rapeseed bee pollen flavonoid extract and vitamin B5 one by one, and finally adjust the pH to a suitable value with triethanolamine, and keep stirring to eliminate bubbles; The preparation of the flavonoid extract in rape bee pollen comprises the following steps: a) extracting flavonoids from bee pollen by ultrasonic method to obtain a crude extract; b) using a rutin standard to determine the flavonoid content in the crude extract to obtain a rape bee pollen flavonoid extract with a flavonoid content of 15%; The mass fraction of glycerol in the facial mask is 12%, the mass fraction of carbomer is 0.4%, and the mass fraction of triethanolamine is 0.5%; The preparation steps of the crude extract in step a) are as follows: 1) Weigh a certain amount of rapeseed bee pollen, add 10 times the volume of 50% ethanol, and soak at room temperature for 2 hours; 2) Ultrasonic extraction was performed for 20 min, three times, and then centrifuged at 2000 r / min for 10 min. The supernatant was fixed to volume with 50% ethanol solution to prepare a crude extract.

2. A rapeseed pollen facial mask, prepared according to the method of claim 1, characterized in that: The facial mask contains 68.25% deionized water, 0.05% sodium hyaluronate, 3% 1,2-propylene glycol, 0.2% methylparaben, 0.5% vitamin B5 and 0.1% hydrogenated castor oil.

3. The use of the rapeseed pollen mask according to claim 2 in preparing a paper mask, characterized in that: The rapeseed pollen facial mask is used to make a paper facial mask, and the paper facial mask is directly attached to the skin during use.

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