Method and system for monitoring the effect of a drug on the interaction of a biological entity with a fluorescent probe

Abstract

The application provides a method for monitoring the effect of a drug on the interaction between a biological body and a fluorescent probe, the method comprising: continuously injecting a first solution comprising a fluorescent probe into a fluorescent chamber and a microfluidic chamber of a microfluidic chip; the fluorescent probe is at least one in kind; at a first time, continuously injecting a solution comprising the fluorescent probe and a drug into the fluorescent chamber and the microfluidic chamber; the drug is at least one in kind; based on the imaging information corresponding to the fluorescent chamber and the microfluidic chamber at each time unit respectively, determining the effect of the drug on the interaction between the biological body and the fluorescent probe; wherein the microfluidic chamber comprises the biological body.

Claims

1. A method for monitoring the effect of drugs on organisms and the interaction of fluorescent probes, characterized in that, The method includes: A first solution comprising at least two fluorescent probes with different emission wavelengths is continuously injected into the fluorescent chamber and the microfluidic chamber of the microfluidic chip; At a first moment, a second solution comprising at least two fluorescent probes and a drug with different emission wavelengths is continuously injected into the fluorescent chamber and the microfluidic chamber; the drug is of at least one type. At the second moment, a base solution is continuously injected into the fluorescence chamber and the microfluidic chamber, the base solution not containing the fluorescent probe and the drug; or, at the second moment, a fluorescent probe solution not containing the drug is continuously injected into the fluorescence chamber and the microfluidic chamber. Based on the imaging information corresponding to the fluorescence chamber and the microfluidic chamber at each time unit, the effect of the drug on the organism and each of the fluorescent probes is determined. The microfluidic chamber contains the organism; the fluorescent chamber does not contain any organism. The determination of the effect of the drug on the interaction between the organism and the fluorescent probe based on the imaging information corresponding to each time unit of the fluorescence chamber and the microfluidic chamber includes: The process involves determining first reference imaging information for the fluorescence chamber at each time unit and first sample imaging information for the microfluidic chamber at each time unit; determining first concentration information of the fluorescent probe within the fluorescence chamber based on the first reference imaging information; determining second concentration information of the fluorescent probe within the microfluidic chamber based on the first sample imaging information; and determining the interaction between the organism and the fluorescent probe based on the first concentration information and the second concentration information.

2. The method according to claim 1, characterized in that, The method of determining the effect of the drug on the interaction between the organism and the fluorescent probe based on the imaging information corresponding to the fluorescence chamber and the microfluidic chamber at each time unit can also be: Based on the imaging detector acquiring the first reference imaging information of the fluorescence chamber in each time unit and the first sample imaging information of the microfluidic chamber in each time unit during the time interval of continuous injection of the first solution; Based on the imaging detector acquiring the second reference imaging information of the fluorescence chamber and the second sample imaging information of the microfluidic chamber in each time unit during the time interval of continuous injection of the second solution; The effect of the drug on the interaction between the organism and the fluorescent probe is determined based on the first reference imaging information, the first sample imaging information, the second reference imaging information, and the second sample imaging information.

3. The method according to claim 1, characterized in that, The method of determining the effect of the drug on the interaction between the organism and the fluorescent probe based on the imaging information corresponding to each time unit of the fluorescence chamber and the microfluidic chamber can also be: Based on the imaging detector continuously acquiring the third reference imaging information of the fluorescence chamber and the third sample imaging information of the microfluidic chamber in each time unit during the time interval of the injected base solution; The kinetic information of the fluorescent probe after interference by the drug is determined based on the third reference imaging information and the third sample imaging information.

4. The method according to claim 1, characterized in that, The types of drugs injected into the different microfluidic chambers may be the same or different.

5. The method according to claim 1, characterized in that, The concentration of the drug injected into different microfluidic chambers may be the same or different.

6. A system for monitoring the effects of drugs on organisms and the interaction of fluorescent probes, characterized in that, The system includes: A fluid control unit is configured to control the continuous injection of a first solution comprising at least two fluorescent probes with different emission wavelengths into a fluorescence chamber and a microfluidic chamber of a microfluidic chip; at a first moment, controlling the continuous injection of a second solution comprising the at least two fluorescent probes with different emission wavelengths and a drug into the fluorescence chamber and the microfluidic chamber; wherein the drug is of at least one type; at a second moment, controlling the continuous injection of a base solution into the fluorescence chamber and each of the microfluidic chambers, wherein the base solution does not contain the fluorescent probes and the drug; or, at a second moment, controlling the continuous injection of a fluorescent probe solution excluding the drug into the fluorescence chamber and the microfluidic chamber. An imaging detector is used to acquire imaging information of the injected solution in the fluorescence chamber and the microfluidic chamber at each time unit; the microfluidic chamber includes the organism; the fluorescence chamber does not contain any organism. A microfluidic chip for determining the effect of the drug on the organism and each of the fluorescent probes based on the imaging information; Specifically, the microfluidic chip is used to determine the first reference imaging information of the fluorescence chamber at each time unit and the first sample imaging information of the microfluidic chamber at each time unit; to determine the first concentration information of the fluorescent probe in the fluorescence chamber based on the first reference imaging information; to determine the second concentration information of the fluorescent probe in the microfluidic chamber based on the first sample imaging information; and to determine the interaction between the organism and the fluorescent probe based on the first concentration information and the second concentration information.

7. The system according to claim 6, characterized in that, When the microfluidic chip is used to determine the effect of the drug on the organism and each fluorescent probe based on the imaging information, it can also be used to: collect the first reference imaging information of the fluorescent chamber and the first sample imaging information of the microfluidic chamber in each time unit during the time interval of continuous injection of the first solution based on the imaging detector. Based on the imaging detector acquiring the second reference imaging information of the fluorescence chamber and the second sample imaging information of the microfluidic chamber in each time unit during the time interval of continuous injection of the second solution; The effect of the drug on the interaction between the organism and the fluorescent probe is determined based on the first reference imaging information, the first sample imaging information, the second reference imaging information, and the second sample imaging information.

8. The system according to claim 6, characterized in that, When the microfluidic chip is used to determine the effect of the drug on the organism and each of the fluorescent probes based on the imaging information, it can also be used to: based on the imaging detector continuously collecting the third reference imaging information of the fluorescent chamber and the third sample imaging information of the microfluidic chamber in each time unit during the time interval of the injection of the base solution. The kinetic information of the fluorescent probe after interference by the drug is determined based on the third reference imaging information and the third sample imaging information.

9. The system according to claim 6, characterized in that, The types of drugs injected into the different microfluidic chambers may be the same or different.

10. The system according to claim 6, characterized in that, The concentration of the drug injected into different microfluidic chambers may be the same or different.

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