A high-throughput parallel time sequential double-staining mechanism and a staining method

Abstract

This invention provides a high-throughput parallel sequential dual staining mechanism and method. The mechanism includes an A-staining zone where an A-staining component performs a first staining process on slides containing prepared samples; and a B-staining zone where a B-staining component performs a second staining process on the slides containing the first-stained samples. A slide turntable, connected to a stepper motor, continuously transports the slides containing the first-stained samples from the A-staining zone to the B-staining zone. Both staining processes are performed simultaneously using a drop-dyeing method, significantly reducing staining time and preventing contamination of the staining solution. Furthermore, the staining process requires only the placement and removal of the prepared smears, eliminating the need for manual intervention and greatly reducing labor costs.

Description

Technical Field

[0001] This invention relates to a staining mechanism and a staining method, specifically, to a high-throughput parallel sequential dual staining mechanism and a staining method. Background Technology

[0002] In traditional automated staining devices for cells or other tissue smears, the commonly used techniques are multi-slide staining and single-slide staining. Multi-slide staining typically involves placing the prepared smear in a device, then immersing the device in a staining bath for staining. After staining, the smear is removed for the next step. The staining solution in the staining bath is reused, leading to contamination and decreased staining quality after repeated use, severely impacting clinical pathological diagnosis. Furthermore, in practice, due to emergency situations such as patient visits, several or even a single smear needs to be stained promptly, reducing the effective use of staining solution and resulting in waste. Single-slide staining, on the other hand, stains smears one by one in individual staining baths. While this design mitigates some of the drawbacks of multi-slide staining, single-slide staining is slower and cannot meet the current high-volume staining demands in clinical practice.

[0003] Both of the aforementioned techniques, whether multi-slide staining or single-slide staining, are only applicable to staining with a single staining solution. They cannot meet the staining needs of dual or multiple staining methods currently available on the market. Summary of the Invention

[0004] In order to overcome the above-mentioned defects and deficiencies in the prior art, the present invention provides a high-throughput parallel temporal dual staining mechanism and staining method.

[0005] To address the aforementioned technical problems, this invention provides a high-throughput parallel temporal dual staining mechanism, comprising:

[0006] The A staining solution staining area provides the A staining solution staining assembly to perform the first staining process on the glass slides after the sample preparation is completed;

[0007] The B-staining solution staining area provides a B-staining solution staining assembly to perform a second staining process on slides that have completed the first staining step; and...

[0008] The slide turntable, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located.

[0009] Furthermore, it also includes a rinsing zone, located between the A staining zone and the B staining zone, providing a rinsing assembly to perform a rinsing process on the slides that have completed the first staining, in order to remove excess A staining solution.

[0010] Furthermore, the A staining solution staining assembly includes an A staining solution dropper, which performs a first staining process on the glass slide.

[0011] Furthermore, the A staining solution dropper has at least one outlet.

[0012] Furthermore, the A staining solution dropper has at least two outlets, arranged in a row along the width of the slide.

[0013] Furthermore, the A staining solution staining assembly also includes a transmission device 1, which transports the A staining solution dropper from its initial position to the A staining solution staining area to perform the first staining process, and then resets it after completion.

[0014] Furthermore, the transmission device is equipped with a contact plate, and a slotted photoelectric switch is located at the initial position. When the contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed.

[0015] Furthermore, the transmission device includes a transmission wheel, a transmission belt, and a connector. Two transmission wheels are fixed on the mounting plate, and the transmission belt connects the two transmission wheels. One of the transmission wheels serves as the driving wheel and is connected to a stepper motor. The mounting plate is provided with a drip port directly above the workstation where the dyeing area of ​​dyeing solution A is located. The drip head of dyeing solution A is placed inside the drip port and fixed to the transmission belt through the connector.

[0016] Furthermore, the connector is fixed to the transmission belt by a clip.

[0017] Furthermore, a connecting plate is fixed on the connecting member, and the A staining solution dropper is fixed on the connecting plate.

[0018] Furthermore, an L-shaped mounting plate is fixed on the mounting plate at the position corresponding to the movement trajectory of the connector, and a guide rail is fixed on the L-shaped mounting plate. The connector is provided with a guide groove that matches the guide rail at the position corresponding to the guide rail.

[0019] Furthermore, the B staining solution assembly includes a B staining solution dropper, which performs a second staining process on the slide that has completed the first staining.

[0020] Furthermore, the B staining solution dropper is fixed to the mounting plate at a position directly opposite the B staining solution staining area via a connector.

[0021] Furthermore, the rinsing assembly includes a rinsing head that performs water rinsing and removes excess liquid from the slide after the first staining.

[0022] Furthermore, the blow-wash head includes several water washing holes and several air blowing holes.

[0023] Furthermore, several water washing holes are arranged in one row along the width of the slide, and several air blowing holes are arranged in another row along the width of the slide.

[0024] Furthermore, the blowing and washing assembly also includes a second transmission device, which transmits the blowing and washing head from its initial position to the blowing and washing area to perform water washing and remove excess liquid processes, and then resets it after completion.

[0025] Furthermore, the transmission device is equipped with a second contact plate, and a slotted photoelectric switch is provided at the initial position. When the second contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed.

[0026] Furthermore, the transmission device 2 includes a transmission wheel 2, a transmission belt 2, and a connector 2. The two transmission wheels 2 are fixed on the mounting plate, and the transmission belt 2 connects the two transmission wheels 2. One of the transmission wheels 2 serves as the driving wheel and is connected to the stepper motor 2. The mounting plate is provided with a blowing port directly above the workstation where the blowing and washing area is located. The blowing and washing head is placed inside the blowing and washing port and fixed to the transmission belt 2 through the connector 2.

[0027] Furthermore, the second connector is fixed to the second transmission belt by the second clip.

[0028] Furthermore, a connecting plate is fixed to the connecting member two, and the blow-drying head is fixed to the connecting plate two.

[0029] Furthermore, an L-shaped mounting plate two is fixed at the position corresponding to the movement trajectory of the connector two on the mounting plate, and a guide rail two is fixed on the L-shaped mounting plate two. The connector two is provided with a guide groove two that matches the guide rail two at the position corresponding to the guide rail two.

[0030] This invention also provides a high-throughput parallel temporal double staining method:

[0031] The A staining solution staining area provides the A staining solution staining component to perform the first staining on the glass slides after the sample preparation is completed;

[0032] The slide turntable, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located.

[0033] The B staining solution staining area provides the B staining solution staining assembly to perform a second staining on the slides that have completed the first staining.

[0034] Furthermore, it also includes a rinsing process, which is completed by a rinsing zone; the rinsing zone is located between the A staining zone and the B staining zone, and provides a rinsing assembly to perform a rinsing process on the slides that have completed the first staining to remove excess A staining solution.

[0035] Furthermore, it also includes a transmission process for the A staining solution staining area, which transmits the A staining solution dropper, which performs the first staining process, from its initial position to the A staining solution staining area, and returns to its initial position after completing the first staining process, thus completing the reset.

[0036] Furthermore, the slide turntable moves the slide from one station to another and holds it for a fixed period of time, during which the transmission process of the A staining solution staining area is completed.

[0037] Furthermore, it also includes a transmission process in the blowing and washing area, which transmits the blowing and washing head that performs the blowing and washing process from the initial position to the blowing and washing area, and returns it to the initial position after completing the blowing and washing process to complete the reset.

[0038] Furthermore, the slide turntable moves the slide from one station to another and holds it for a fixed period of time, during which the transmission process in the rinsing zone is completed.

[0039] Furthermore, the A staining solution staining assembly includes an A staining solution dropper, which performs the first staining process on the glass slide.

[0040] Furthermore, the A staining solution dropper has at least one outlet.

[0041] Furthermore, the A staining solution dropper has at least two outlets, arranged in a row along the width of the slide.

[0042] Furthermore, the A staining solution staining assembly also includes a transmission device 1, which transports the A staining solution dropper from its initial position to the A staining solution staining area to perform the first staining process, and then resets it after completion.

[0043] Furthermore, the transmission device is equipped with a contact plate, and a slotted photoelectric switch is located at the initial position. When the contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed.

[0044] Furthermore, the transmission device includes a transmission wheel, a transmission belt, and a connector. Two transmission wheels are fixed on the mounting plate, and the transmission belt connects the two transmission wheels. One of the transmission wheels serves as the driving wheel and is connected to a stepper motor. The mounting plate is provided with a drip port directly above the workstation where the dyeing area of ​​dyeing solution A is located. The drip head of dyeing solution A is placed inside the drip port and fixed to the transmission belt through the connector.

[0045] Furthermore, the connector is fixed to the transmission belt by a clip.

[0046] Furthermore, a connecting plate is fixed on the connecting member, and the A staining solution dropper is fixed on the connecting plate.

[0047] Furthermore, an L-shaped mounting plate is fixed on the mounting plate at the position corresponding to the movement trajectory of the connector, and a guide rail is fixed on the L-shaped mounting plate. The connector is provided with a guide groove that matches the guide rail at the position corresponding to the guide rail.

[0048] Furthermore, the B staining solution assembly includes a B staining solution dropper, which performs a second staining process on the slide that has completed the first staining.

[0049] Furthermore, the B staining solution dropper is fixed to the mounting plate at a position directly opposite the B staining solution staining area via a connector.

[0050] Furthermore, the rinsing assembly includes a rinsing head that performs water rinsing and removes excess liquid from the slide after the first staining.

[0051] Furthermore, the blow-wash head includes several water washing holes and several air blowing holes.

[0052] Furthermore, several water washing holes are arranged in one row along the width of the slide, and several air blowing holes are arranged in another row along the width of the slide.

[0053] Furthermore, the blowing and washing assembly also includes a second transmission device, which transmits the blowing and washing head from its initial position to the blowing and washing area to perform water washing and remove excess liquid processes, and then resets it after completion.

[0054] Furthermore, the transmission device is equipped with a second contact plate, and a slotted photoelectric switch is provided at the initial position. When the second contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed.

[0055] Furthermore, the transmission device 2 includes a transmission wheel 2, a transmission belt 2, and a connector 2. The two transmission wheels 2 are fixed on the mounting plate, and the transmission belt 2 connects the two transmission wheels 2. One of the transmission wheels 2 serves as the driving wheel and is connected to the stepper motor 2. The mounting plate is provided with a blowing port directly above the workstation where the blowing and washing area is located. The blowing and washing head is placed inside the blowing and washing port and fixed to the transmission belt 2 through the connector 2.

[0056] Furthermore, the second connector is fixed to the second transmission belt by the second clip.

[0057] Furthermore, a connecting plate is fixed to the connecting member two, and the blow-drying head is fixed to the connecting plate two.

[0058] Furthermore, an L-shaped mounting plate two is fixed at the position corresponding to the movement trajectory of the connector two on the mounting plate, and a guide rail two is fixed on the L-shaped mounting plate two. The connector two is provided with a guide groove two that matches the guide rail two at the position corresponding to the guide rail two.

[0059] The beneficial technical effects achieved by this invention are as follows: This invention provides a high-throughput parallel sequential dual staining mechanism and method. A slide shredder continuously rotates the slides, while the first staining step performed by the A staining solution component and the second staining step performed by the B staining solution component are performed simultaneously. Both staining steps are implemented using a drop-dyeing method, which not only greatly saves staining time but also prevents contamination of the staining solution. Furthermore, only the prepared smear needs to be placed in or removed; the staining process does not require manual intervention, thus greatly reducing manpower. Moreover, a rinsing step is provided between the two staining steps. The water rinsing step removes any remaining A staining solution, preventing contamination of the staining result; simultaneously, the air rinsing step removes any flowing liquid, preventing dilution of the B staining solution in the second staining step due to the presence of liquid, which would affect the staining result. Attached Figure Description

[0060] Figure 1 A schematic diagram of the structure of an embodiment of the high-throughput parallel temporal dual staining mechanism of the present invention;

[0061] Figure 2 Another structural schematic diagram of an embodiment of the high-throughput parallel temporal dual staining mechanism of the present invention;

[0062] Figure 3 A top view schematic diagram of an embodiment of the high-throughput parallel temporal dual staining mechanism of the present invention;

[0063] Figure 4 A schematic diagram of the A-staining solution staining component of the high-throughput parallel-temporal dual staining mechanism of the present invention;

[0064] Figure 5 A schematic diagram of the rinsing assembly of a throughput parallel sequential dual staining mechanism of the present invention;

[0065] Figure 6 A schematic diagram of the B staining solution staining component of the high-throughput parallel-temporal dual staining mechanism of the present invention;

[0066] Figure 7 A schematic diagram of an embodiment of the high-throughput parallel-temporal dual-staining mechanism of the present invention;

[0067] Figure 8 A schematic diagram of another embodiment of the high-throughput parallel temporal dual staining mechanism of the present invention.

[0068] The components include: 1. Slide turntable; 2. Mounting plate; 3. A staining solution assembly; 301. Dropper; 302. L-shaped mounting plate; 303. Guide rail; 304. A staining solution dropper; 305. Slotted photoelectric switch; 306. Stepper motor; 307. Contact plate; 308. Connector; 309. Drive wheel; 310. Drive belt; 311. Guide groove; 312. Clip; 313. Connecting plate; 4. Washing assembly; 401. Blow-suction port; 402. Water washing hole; 403. Blow-wash head; 404. Slotted photoelectric switch; 405. Contact plate; 406. Connector; 407. Guide groove; 408. Clip; 409. Guide rail; 410. Drive belt; 4011. L-shaped mounting plate; 412. Drive wheel; 413. Air hole; 414. Connecting plate. Detailed Implementation

[0069] The present invention will be further described below with reference to specific embodiments. These embodiments are only used to more clearly illustrate the technical solutions of the present invention and should not be construed as limiting the scope of protection of the present invention.

[0070] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0071] This invention provides a high-throughput parallel temporal dual staining mechanism, comprising:

[0072] The staining area of ​​staining solution A provides three staining components of staining solution A to perform the first staining process on glass slides that have completed the preparation of the sample to be tested.

[0073] The B-staining solution staining area provides 5 pairs of B-staining solution staining components for performing the second staining process on slides that have completed the first staining step; and...

[0074] The slide turntable 1, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located.

[0075] As an exemplary embodiment of the present invention, the invention may further include a rinsing zone positioned between the A-staining zone and the B-staining zone. A rinsing assembly 4 is provided to perform a rinsing process on the slides after the first staining step to remove excess A-staining solution. In specific implementation, the rinsing zone completes two processes: 1. a water rinsing process; 2. an air blowing process. The water rinsing process is to thoroughly clean away any residual A-staining solution from the first staining step to ensure the staining effect; the air blowing process is to blow away any remaining water after rinsing to prevent dilution of the B-staining solution concentration in the next staining step, which would affect the staining effect.

[0076] In the staining area of ​​staining solution A, as an exemplary embodiment disclosed in this invention, such as... Figures 1-4As shown, the A staining solution staining assembly 3 includes an A staining solution dropper 304, which performs the first staining process on the glass slide 6. As a more preferred technical solution, the A staining solution dropper 304 has at least one outlet. More preferably, the A staining solution dropper 304 has at least two outlets, arranged in a row along the width of the glass slide 6. Having two or more outlets in a row allows the staining solution to be evenly distributed on the glass slide during the staining process, preventing incomplete staining of the sample.

[0077] As a specific embodiment disclosed by example of the present invention, the A staining solution staining assembly 3 may further include a transmission device 1, which conveys the A staining solution dropper 304 from its initial position to the A staining solution staining area to perform the first staining process, and then resets it after completion. The transmission begins, and the A staining solution dropper 304 is simultaneously activated, ensuring that the entire slide is covered with the staining solution.

[0078] As an exemplary embodiment of the present invention, a contact plate 307 is provided on the transmission device 1, and a slotted photoelectric switch 305 is provided at the initial position. When the contact plate 307 moves onto the slotted photoelectric switch 305 as the transmission device 1 moves, the transmission device 1 stops working and the reset is completed.

[0079] As an exemplary embodiment of the present invention, the transmission device includes a transmission wheel 309, a transmission belt 310, and a connector 308. Two transmission wheels 309 are fixed on the mounting plate 2, and the transmission belt 310 connects the two transmission wheels 309. One of the transmission wheels 309 serves as the driving wheel and is connected to a stepper motor 306. The mounting plate 2 is provided with a drip port 301 directly above the workstation where the A staining solution is located. The A staining solution dropper 304 is placed inside the drip port 301 and fixed to the transmission belt 310 by the connector 308. After the stepper motor 306 is started, the transmission wheel 309 drives the transmission belt 310 to rotate. Therefore, the A staining solution dropper 304 fixed on the transmission belt 310 moves within the drip port 301, and the A staining solution dripping from the A staining solution dropper 304 falls onto the glass slide 6. Preferably, the connector 308 is fixed to the transmission belt 310 by a clip 312, which has a simple structure and is easy to install. More preferably, a connecting plate 313 is fixed to the connector 308, and the A staining solution dropper is fixed to the connecting plate 313. More preferably, an L-shaped mounting plate 302 is fixed on the mounting plate 2 at a position corresponding to the movement trajectory of the connector 308, a guide rail 303 is fixed on the L-shaped mounting plate 302, and the connector 308 is provided with a guide groove 311 that matches the guide rail 303 at a position corresponding to the guide rail 303.

[0080] As an exemplary embodiment disclosed in this invention, such as Figure 6As shown, the B staining solution assembly includes a B staining solution dropper 502, which performs a second staining process on the slide 6 that has completed the first staining. Preferably, the B staining solution dropper 502 is fixed to the mounting plate 2 directly opposite the B staining solution area by a connector 501. When the slide that has completed the first staining process rotates to the position directly below it, the B staining solution dropper is activated to perform B staining.

[0081] As an exemplary embodiment disclosed in this invention, for example... Figure 5 As shown, the rinsing assembly 4 includes a rinsing head 403, which performs water rinsing and removes excess liquid from the slide 6 after the first staining. In specific embodiments, the rinsing head 403 can be either a separate unit or a single unit. A separate unit means that the air blowing part and the water washing part are two independent structures, while a single unit means that the air blowing part and the water washing part are combined to form a structure with both functions. Preferably, the rinsing head is a single unit, including a plurality of water washing holes 402 and a plurality of air blowing holes 413. More preferably, the plurality of water washing holes 402 are arranged in one row along the width direction of the slide 6, and the plurality of air blowing holes 413 are arranged in another row along the width direction of the slide. This allows for cleaning of the entire area of ​​the slide.

[0082] As an exemplary embodiment of the present invention, the rinsing assembly 4 further includes a transmission device 2, which transmits the rinsing head 403 from its initial position to the rinsing area to perform water rinsing and removal of excess liquid, and then resets it after completion. The transmission begins simultaneously with the rinsing head being turned on, ensuring that the entire slide is covered with the staining solution.

[0083] As an exemplary embodiment of the present invention, a second contact plate 405 is provided on the transmission device, and a second slotted photoelectric switch 404 is provided at the initial position. When the second contact plate 405 moves onto the second slotted photoelectric switch 404 as the transmission device moves, the transmission device stops working and the reset is completed.

[0084] As a specific embodiment of the present invention, the transmission device two includes a transmission wheel two 412, a transmission belt two 410, and a connector two 406. The two transmission wheels two 412 are fixed on the mounting plate 2, and the transmission belt two 410 connects the two transmission wheels two 412. One of the transmission wheels two 412 serves as the driving wheel and is connected to the stepper motor two. The mounting plate 2 is provided with a blowing port 401 directly above the station where the blowing area is located. The blowing head 403 is placed in the blowing port 401 and fixed to the transmission belt two 410 through the connector two 406. After the stepper motor two, which drives the transmission wheel two 412, starts, the transmission wheel two 412 drives the transmission belt two 410 to rotate. Therefore, the blowing head 403 fixed on the transmission belt two 410 moves in the blowing port 401. The cleaning liquid coming out of the water washing hole 402 on the blowing head 403 falls onto the glass slide to clean the residual A staining solution, and the gas coming out of the air blowing hole 413 blows off the remaining liquid. Preferably, the second connector 406 is fixed to the second transmission belt 410 by a second clip 408. More preferably, a second connecting plate 414 is fixed to the second connector 408, and the blow-dry head 403 is fixed to the second connecting plate 414. More preferably, an L-shaped mounting plate 411 is fixed on the mounting plate 2 at a position corresponding to the movement trajectory of the second connector 406, a second guide rail 409 is fixed on the L-shaped mounting plate 411, and the second connector 406 is provided with a guide groove 407 that matches the second guide rail 409 at a position corresponding to the second guide rail 409.

[0085] The high-throughput parallel temporal dual staining mechanism provided by this invention has the following parallel temporal principle:

[0086] To better illustrate the principle, as an exemplary embodiment of this invention, 10 slides are stained in parallel each time. The station containing the staining area of ​​staining solution A is designated as position 1, and the slide is designated as number 1. The slides are numbered sequentially clockwise from 1 to 10, and the station numbers are numbered counterclockwise from 1 to 10. The station containing the staining area of ​​staining solution B is designated as position 6, and the station containing the rinsing area is designated as position 5. The schematic diagram is shown below. Figure 7 As shown. After slide #4 is stained, the slide turntable rotates once, and slide #1 moves to position 5. At this time, the A staining solution unit performs the first staining process on slide #5, and the rinsing unit performs the rinsing process on slide #1; both processes are performed simultaneously. After another rotation, slide #1 moves to position 6, slide #2 moves to position 5, and slide #6 moves to position 1. At this time, the A staining solution unit performs the first staining process on slide #6, the rinsing unit performs the rinsing process on slide #2, and the B staining solution unit performs the second staining process on slide #1; all three processes are performed simultaneously, resulting in a high staining rate. Experimental results show that this staining mechanism can achieve a staining capacity of 1600 slides / day.

[0087] As a specific embodiment of the present invention, to facilitate the overall handling of glass slides, 12 stations can be set on the glass slide turntable, with one empty space between every 5 glass slides. The parallel timing principle diagram is as follows. Figure 8 As shown above, the detailed analysis will not be repeated here.

[0088] This invention also provides a high-throughput parallel temporal double staining method, which specifically includes the following steps:

[0089] The A staining solution staining area provides the A staining solution staining component to perform the first staining on the glass slides after the sample preparation is completed;

[0090] The slide turntable, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located.

[0091] The B staining solution staining area provides the B staining solution staining assembly to perform a second staining on the slides that have completed the first staining.

[0092] More preferably, it also includes a rinsing process, which is completed by a rinsing zone; the rinsing zone is located between the A staining zone and the B staining zone, and provides a rinsing assembly to perform a rinsing process on the slides that have completed the first staining to remove excess A staining solution.

[0093] More preferably, it also includes a transmission process for the A staining solution staining area, which transmits the A staining solution dropper, which performs the first staining process, from its initial position to the A staining solution staining area, and returns to its initial position after completing the first staining process, thus completing the reset. The transmission begins simultaneously with the A staining solution dropper 304 being turned on, ensuring that the entire slide is covered with the staining solution.

[0094] Ideally, the slide turntable moves the slide from one station to another, where it remains for a fixed time, such as 2s, 3s, 4s, or 5s. It is important to note that the transfer process in the A staining solution staining area and the first staining process are completed within this dwell time.

[0095] More preferably, it also includes a transmission process for the rinsing zone, which transports the rinsing head, which performs the rinsing process, from its initial position to the rinsing zone, and returns it to its initial position after completing the rinsing process, thus completing the reset. The transmission starts simultaneously with the rinsing head, ensuring that the entire slide is covered with the staining solution.

[0096] The slide turntable moves the slide from one station to another, where it remains for a fixed time, which can be selected as 2s, 3s, 4s, 5s, etc. It is important to note that the transfer process in the A staining solution staining area and the first staining process are completed within this dwell time.

[0097] As an exemplary embodiment disclosed in this invention, such as Figures 1-4 As shown, the A staining solution staining assembly 3 includes an A staining solution dropper 304, which performs the first staining process on the glass slide 6. As a more preferred technical solution, the A staining solution dropper 304 has at least one outlet. More preferably, the A staining solution dropper 304 has at least two outlets, arranged in a row along the width of the glass slide 6. Having two or more outlets in a row allows the staining solution to be evenly distributed on the glass slide during the staining process, preventing incomplete staining of the sample.

[0098] As a specific embodiment disclosed by example of the present invention, the A staining solution staining assembly 3 may further include a transmission device 1, which conveys the A staining solution dropper 304 from its initial position to the A staining solution staining area to perform the first staining process, and then resets it after completion. The transmission begins, and the A staining solution dropper 304 is simultaneously activated, ensuring that the entire slide is covered with the staining solution.

[0099] As an exemplary embodiment of the present invention, a contact plate 307 is provided on the transmission device 1, and a slotted photoelectric switch 305 is provided at the initial position. When the contact plate 307 moves onto the slotted photoelectric switch 305 as the transmission device 1 moves, the transmission device 1 stops working and the reset is completed.

[0100] As an exemplary embodiment of the present invention, the transmission device includes a transmission wheel 309, a transmission belt 310, and a connector 308. Two transmission wheels 309 are fixed on the mounting plate 2, and the transmission belt 310 connects the two transmission wheels 309. One of the transmission wheels 309 serves as the driving wheel and is connected to a stepper motor 306. The mounting plate 2 is provided with a drip port 301 directly above the workstation where the A staining solution is located. The A staining solution dropper 304 is placed inside the drip port 301 and fixed to the transmission belt 310 by the connector 308. After the stepper motor 306 is started, the transmission wheel 309 drives the transmission belt 310 to rotate. Therefore, the A staining solution dropper 304 fixed on the transmission belt 310 moves within the drip port 301, and the A staining solution dripping from the A staining solution dropper 304 falls onto the glass slide 6. Preferably, the connector 308 is fixed to the transmission belt 310 by a clip 312, which has a simple structure and is easy to install. More preferably, a connecting plate 313 is fixed to the connector 308, and the A staining solution dropper is fixed to the connecting plate 313. More preferably, an L-shaped mounting plate 302 is fixed on the mounting plate 2 at a position corresponding to the movement trajectory of the connector 308, a guide rail 303 is fixed on the L-shaped mounting plate 302, and the connector 308 is provided with a guide groove 311 that matches the guide rail 303 at a position corresponding to the guide rail 303.

[0101] As an exemplary embodiment disclosed in this invention, such as Figure 6 As shown, the B staining solution assembly includes a B staining solution dropper 502, which performs a second staining process on the slide 6 that has completed the first staining. Preferably, the B staining solution dropper 502 is fixed to the mounting plate 2 directly opposite the B staining solution area by a connector 501. When the slide that has completed the first staining process rotates to the position directly below it, the B staining solution dropper is activated to perform B staining.

[0102] As an exemplary embodiment disclosed in this invention, for example... Figure 5 As shown, the rinsing assembly 4 includes a rinsing head 403, which performs water rinsing and removes excess liquid from the slide 6 after the first staining. In specific embodiments, the rinsing head 403 can be either a separate unit or a single unit. A separate unit means that the air blowing part and the water washing part are two independent structures, while a single unit means that the air blowing part and the water washing part are combined to form a structure with both functions. Preferably, the rinsing head is a single unit, including a plurality of water washing holes 402 and a plurality of air blowing holes 413. More preferably, the plurality of water washing holes 402 are arranged in one row along the width direction of the slide 6, and the plurality of air blowing holes 413 are arranged in another row along the width direction of the slide. This allows for cleaning of the entire area of ​​the slide.

[0103] As an exemplary embodiment of the present invention, the rinsing assembly 4 further includes a transmission device 2, which transmits the rinsing head 403 from its initial position to the rinsing area to perform water rinsing and removal of excess liquid, and then resets it after completion. The transmission begins simultaneously with the rinsing head being turned on, ensuring that the entire slide is covered with the staining solution.

[0104] As an exemplary embodiment of the present invention, a second contact plate 405 is provided on the transmission device, and a second slotted photoelectric switch 404 is provided at the initial position. When the second contact plate 405 moves onto the second slotted photoelectric switch 404 as the transmission device moves, the transmission device stops working and the reset is completed.

[0105] As a specific embodiment of the present invention, the transmission device two includes a transmission wheel two 412, a transmission belt two 410, and a connector two 406. The two transmission wheels two 412 are fixed on the mounting plate 2, and the transmission belt two 410 connects the two transmission wheels two 412. One of the transmission wheels two 412 serves as the driving wheel and is connected to the stepper motor two. The mounting plate 2 is provided with a blowing port 401 directly above the station where the blowing area is located. The blowing head 403 is placed in the blowing port 401 and fixed to the transmission belt two 410 through the connector two 406. After the stepper motor two, which drives the transmission wheel two 412, starts, the transmission wheel two 412 drives the transmission belt two 410 to rotate. Therefore, the blowing head 403 fixed on the transmission belt two 410 moves in the blowing port 401. The cleaning liquid coming out of the water washing hole 402 on the blowing head 403 falls onto the glass slide to clean the residual A staining solution, and the gas coming out of the air blowing hole 413 blows off the remaining liquid. Preferably, the second connector 406 is fixed to the second transmission belt 410 by a second clip 408. More preferably, a second connecting plate 414 is fixed to the second connector 408, and the blow-dry head 403 is fixed to the second connecting plate 414. More preferably, an L-shaped mounting plate 411 is fixed on the mounting plate 2 at a position corresponding to the movement trajectory of the second connector 406, a second guide rail 409 is fixed on the L-shaped mounting plate 411, and the second connector 406 is provided with a guide groove 407 that matches the second guide rail 409 at a position corresponding to the second guide rail 409.

[0106] The present invention has been disclosed above with reference to preferred embodiments, but it is not intended to limit the present invention. All technical solutions obtained by adopting equivalent substitutions or equivalent transformations fall within the protection scope of the present invention.

Claims

1. A high-throughput parallel temporal dual staining mechanism, characterized in that, include: The A-staining solution staining area provides an A-staining solution staining assembly to perform the first staining process on the glass slides after sample preparation; the rinsing area, located between the A-staining solution staining area and the B-staining solution staining area, provides a rinsing assembly to perform a rinsing process on the glass slides after the first staining process to remove excess A-staining solution; the A-staining solution staining assembly includes an A-staining solution dropper and a transmission device. The A-staining solution dropper performs the first staining process on the glass slides, and the transmission device moves the A-staining solution dropper from its initial position to the A-staining solution staining area to perform the first staining process, and then resets after completion; A contact plate is provided on the transmission device 1, and a slotted photoelectric switch is provided at the initial position. When the contact plate 1 moves to the slotted photoelectric switch 1 as the transmission device 1 moves, the reset is completed. The transmission device includes a transmission wheel, a transmission belt, and a connector. The two transmission wheels are fixed on the mounting plate, and the transmission belt connects the two transmission wheels. One of the transmission wheels is connected to the stepper motor as the driving wheel. The mounting plate is provided with a drip port directly above the work station where the dyeing area of ​​dyeing solution A is located. The drip head of dyeing solution A is placed in the drip port and fixed to the transmission belt through the connector. The B-staining solution staining area provides a B-staining solution staining assembly to perform a second staining process on slides that have completed the first staining. The B-staining solution staining assembly includes a B-staining solution dropper fixed to a position on the mounting plate directly opposite the B-staining solution staining area via a connector three, and performs the second staining process on slides that have completed the first staining. The rinsing assembly includes a rinsing head and a transmission device two. The rinsing head performs a water rinsing and removes excess liquid from the slides that have completed the first staining. The transmission device two transports the rinsing head from its initial position to the rinsing area to perform a water rinsing and remove excess liquid, and then resets it after completion. The transmission device is equipped with a second contact plate, and a slotted photoelectric switch is located at the initial position. When the second contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed. The transmission device 2 includes a transmission wheel 2, a transmission belt 2 and a connector 2. The two transmission wheels 2 are fixed on the mounting plate, and the transmission belt 2 connects the two transmission wheels 2. One of the transmission wheels 2 serves as the driving wheel and is connected to the stepper motor 2. The mounting plate is provided with a blowing port directly above the work station where the blowing and washing area is located. The blowing and washing head is placed in the blowing and washing port and fixed to the transmission belt 2 through the connector 2. The slide turntable, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located.

2. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: The A staining solution dropper has at least one outlet.

3. The high-throughput parallel temporal dual staining mechanism according to claim 2, characterized in that: The A staining solution dropper has at least two outlets, arranged in a row along the width of the slide.

4. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: The connector is fixed to the transmission belt by a clip.

5. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: A connecting plate is fixed on the connector, and the A staining solution dropper is fixed on the connecting plate.

6. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: An L-shaped mounting plate is fixed on the mounting plate at the movement trajectory of the connector. A guide rail is fixed on the L-shaped mounting plate. The connector is provided with a guide groove that matches the guide rail at the position corresponding to the guide rail.

7. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: The blow-dry head includes several water washing holes and several air blowing holes.

8. The high-throughput parallel temporal dual staining mechanism according to claim 7, characterized in that: Several water washing holes are arranged in one row along the width of the slide, and several air blowing holes are arranged in another row along the width of the slide.

9. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: The second connector is fixed to the second transmission belt by the second clip.

10. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: A connecting plate is fixed to the connecting member 2, and the blow-dry head is fixed to the connecting plate 2.

11. The high-throughput parallel temporal dual staining mechanism according to claim 1, characterized in that: An L-shaped mounting plate is fixed on the mounting plate at the location corresponding to the movement trajectory of the connector two. A guide rail is fixed on the L-shaped mounting plate, and a guide groove matching the guide rail is provided at the location of the connector two corresponding to the position of the guide rail two.

12. A high-throughput parallel temporal double staining method, characterized in that: The A staining solution staining area provides the A staining solution staining component to perform the first staining on the glass slides after the sample preparation is completed; The slide turntable, connected to a stepper motor, continuously transports the slides that have completed the first staining step from the station where the A-solution staining area is located to the station where the B-solution staining area is located. The B staining solution staining area provides the B staining solution staining assembly to perform a second staining on the slides that have completed the first staining. The rinsing process is completed by the rinsing zone, which is located between the A-staining solution staining zone and the B-staining solution staining zone. The rinsing zone provides a rinsing assembly to perform the rinsing process on the slides that have completed the first staining step, removing excess A-staining solution. The A-staining solution staining zone's transmission process moves the A-staining solution dropper from its initial position to the A-staining solution staining zone, and after completing the first staining step, it returns to its initial position, completing the reset. The slide turntable moves the slide from one station to another, where it stays for a fixed time, during which the transmission process of the A-staining solution staining zone is completed. The rinsing zone's transmission process moves the rinsing head from its initial position to the rinsing zone, and after completing the rinsing step, it returns to its initial position, completing the reset. The staining assembly for A staining solution includes an A staining solution dropper and a transmission device. The A staining solution dropper performs the first staining process on the glass slide. The transmission device moves the A staining solution dropper from its initial position to the A staining solution staining area to perform the first staining process. After completion, it is reset. A contact plate is provided on the transmission device 1, and a slotted photoelectric switch is provided at the initial position. When the contact plate 1 moves to the slotted photoelectric switch 1 as the transmission device 1 moves, the reset is completed. The transmission device includes a transmission wheel, a transmission belt, and a connector. The two transmission wheels are fixed on the mounting plate, and the transmission belt connects the two transmission wheels. One of the transmission wheels is connected to the stepper motor as the driving wheel. The mounting plate is provided with a drip port directly above the work station where the dyeing area of ​​dyeing solution A is located. The drip head of dyeing solution A is placed in the drip port and fixed to the transmission belt through the connector. The B staining solution staining assembly includes a B staining solution dropper fixed to the mounting plate at a position directly opposite the B staining solution staining area via connector three, which performs a second staining process on the slides that have completed the first staining. The rinsing assembly includes a rinsing head and a transmission device two. The rinsing head performs a water rinsing and removes excess liquid from the slides that have completed the first staining. The transmission device two transports the rinsing head from its initial position to the rinsing area to perform a water rinsing and remove excess liquid, and then resets it after completion. The transmission device is equipped with a second contact plate, and a slotted photoelectric switch is located at the initial position. When the second contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed. The transmission device 2 includes a transmission wheel 2, a transmission belt 2, and a connector 2. The two transmission wheels 2 are fixed on the mounting plate, and the transmission belt 2 connects the two transmission wheels 2. One of the transmission wheels 2 serves as the driving wheel and is connected to the stepper motor 2. The mounting plate is provided with a blowing port directly above the workstation where the blowing and washing area is located. The blowing and washing head is placed inside the blowing and washing port and fixed to the transmission belt 2 through the connector 2.

13. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The A staining solution dropper has at least one outlet.

14. The high-throughput parallel temporal double staining method according to claim 13, characterized in that: The A staining solution dropper has at least two outlets, arranged in a row along the width of the slide.

15. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The connector is fixed to the transmission belt by a clip.

16. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: A connecting plate is fixed on the connector, and the A staining solution dropper is fixed on the connecting plate.

17. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: An L-shaped mounting plate is fixed on the mounting plate at the movement trajectory of the connector. A guide rail is fixed on the L-shaped mounting plate. The connector is provided with a guide groove that matches the guide rail at the position corresponding to the guide rail.

18. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The blow-dry head includes several water washing holes and several air blowing holes.

19. The high-throughput parallel temporal dual staining method according to claim 18, characterized in that: Several water washing holes are arranged in one row along the width of the slide, and several air blowing holes are arranged in another row along the width of the slide.

20. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The blowing and washing assembly also includes a second transmission device, which transmits the blowing and washing head from its initial position to the blowing and washing area to perform water washing and remove excess liquid processes, and then resets it after completion.

21. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The transmission device is equipped with a second contact plate, and a slotted photoelectric switch is located at the initial position. When the second contact plate moves onto the slotted photoelectric switch as the transmission device moves, the reset is completed.

22. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The transmission device 2 includes a transmission wheel 2, a transmission belt 2, and a connector 2. The two transmission wheels 2 are fixed on the mounting plate, and the transmission belt 2 connects the two transmission wheels 2. One of the transmission wheels 2 serves as the driving wheel and is connected to the stepper motor 2. The mounting plate is provided with a blowing port directly above the workstation where the blowing and washing area is located. The blowing and washing head is placed inside the blowing and washing port and fixed to the transmission belt 2 through the connector 2.

23. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: The second connector is fixed to the second transmission belt by the second clip.

24. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: A connecting plate is fixed to the connecting member 2, and the blow-dry head is fixed to the connecting plate 2.

25. The high-throughput parallel temporal dual staining method according to claim 12, characterized in that: An L-shaped mounting plate is fixed on the mounting plate at the location corresponding to the movement trajectory of the connector two. A guide rail is fixed on the L-shaped mounting plate, and a guide groove matching the guide rail is provided at the location of the connector two corresponding to the position of the guide rail two.

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