A magnetic separation cleaning device and method

Abstract

The present invention discloses a magnetic separation cleaning device and method, relating to the technical field of in vitro diagnostic medical devices. The device includes a turntable that can rotate counterclockwise around a central axis; 14 reaction cup placement grooves that can rotate together with the turntable are evenly arranged along the circumferential direction on the top surface of the turntable; along the counterclockwise direction, the positions where each reaction cup placement groove is located are sequentially used as the first to fourteenth workstations, and each workstation does not rotate with the turntable; first permanent magnets are respectively arranged at the third, seventh, and eleventh workstations, second permanent magnets are respectively arranged at the fourth, eighth, and twelfth workstations, and third permanent magnets are respectively arranged at the fifth, ninth, and thirteenth workstations; the permanent magnet placement grooves do not change their positions with the rotation of the turntable. The device of the present invention optimizes the working timing. One sample to be cleaned can be placed every T seconds. In the case of three rounds of cleaning, it takes 14*T seconds for the first sample to complete cleaning, and the time for each subsequent sample to complete cleaning is only separated from the previous sample by T seconds, greatly improving the throughput of cleaning samples.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnostic medical devices, and particularly to a magnetic separation and cleaning device and method. Background Art

[0002] Chemiluminescence immunoassay combines the high sensitivity of chemiluminescence technology with the high specificity of immunological methods to achieve quantitative analysis of substances such as proteins, hormones, and drugs in the human body. Among them, the magnetic separation and cleaning of magnetic bead immune complexes is a key module of immunoassay and also the most complex system in the detection system. The separation speed directly affects the detection speed of the instrument, and the separation effect directly affects the immunoassay result. Therefore, the rapid, non-destructive, and high-purity separation of magnetic bead immune complexes is an important link to ensure the accuracy of immunoassay. Magnetic separation and cleaning usually require four steps: adding cleaning solution, mixing, collecting magnetic beads, and pumping out waste liquid. The usual method is: first add the cleaning solution and mix it to disperse the magnetic bead immune complexes, and the reagents that originally adhered to the magnetic beads but did not participate in the reaction dissolve in the cleaning solution. Move the reaction cup close to the magnet, the magnetic beads are adsorbed on the side wall of the reaction cup, and then all the liquid in the reaction cup is pumped out. Repeat this several times, and the residual reagents in the reaction cup are cleaned. During the cleaning process, it is necessary to ensure that the loss of magnetic beads is as small as possible so that the correct signal value can be obtained in the final test. Therefore, it is necessary to reasonably design the layout and installation method of the magnet.

[0003] It can be seen that the conventional magnetic separation and cleaning device has the defects of long single cleaning time, low cleaning sample throughput, and relatively high magnetic bead loss rate. Therefore, there is an urgent need to provide a new magnetic separation and cleaning device. Summary of the Invention

[0004] The purpose of the present invention is to overcome the defects in the prior art and provide a magnetic separation and cleaning device and method.

[0005] The specific technical solutions adopted by the present invention are as follows:

[0006] In a first aspect, the present invention provides a magnetic separation cleaning device, including a turntable that can rotate counterclockwise around a central axis; 14 reaction cup placement grooves that are evenly arranged along the circumference on the top surface of the turntable and can rotate together with the turntable, and the reaction cup placement grooves are used for placing reaction cups containing magnetic beads to be cleaned; along the counterclockwise direction, the positions where each reaction cup placement groove is located are sequentially used as the first station, the fourth station, the seventh station, the tenth station, the thirteenth station, the second station, the fifth station, the eighth station, the eleventh station, the fourteenth station, the third station, the sixth station, the ninth station and the twelfth station, and the positions indicated by each station are fixed and do not change with the rotation of the turntable; permanent magnet placement grooves for placing first permanent magnets are respectively provided at the reaction cup placement grooves located at the third station, the seventh station and the eleventh station, permanent magnet placement grooves for placing second permanent magnets are respectively provided at the reaction cup placement grooves located at the fourth station, the eighth station and the twelfth station, and permanent magnet placement grooves for placing third permanent magnets are respectively provided at the reaction cup placement grooves located at the fifth station, the ninth station and the thirteenth station; the permanent magnet placement grooves do not change their positions with the rotation of the turntable; there is a gap between each group of corresponding permanent magnet placement grooves and reaction cup placement grooves, and when the reaction cup rotates to the corresponding station, it can be directly opposite to the magnetic pole surface of the permanent magnet.

[0007] Preferably, the reaction cup placement groove has a circular notch, and the permanent magnet placement groove has a square notch.

[0008] Preferably, the first permanent magnet, the second permanent magnet and the third permanent magnet are made of metal alloy material or ferrite material.

[0009] Preferably, the first permanent magnet has a cuboid structure with dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is a surface of 10mm * 16mm.

[0010] Preferably, the second permanent magnet is composed of two vertically spliced N - pole magnets and S - pole magnets, and the plane where the splicing gap is located passes through the central axis of the turntable; the dimensions of both magnets are 5mm * 10mm * 16mm, and the magnetic pole surfaces are both surfaces of 5mm * 16mm. After splicing, the obtained second permanent magnet has dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is a surface of 10mm * 16mm.

[0011] Preferably, the third permanent magnet is composed of two horizontally spliced N - pole magnets and S - pole magnets, and the plane where the splicing gap is located is perpendicular to the central axis of the turntable; the dimensions of both magnets are 10mm * 10mm * 8mm, and the magnetic pole surfaces are both surfaces of 10mm * 8mm. After splicing, the obtained third permanent magnet has dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is a surface of 10mm * 16mm.

[0012] Second aspect, the present invention provides a cleaning method using any of the magnetic separation cleaning devices of the first aspect, specifically as follows:

[0013] The turntable rotates counterclockwise once every T seconds, and each time it rotates through five stations. Reaction cups are placed in sequence, so that the reaction cups are in the order of the first station, the second station, the third station, the fourth station, the fifth station, the sixth station, the seventh station, the eighth station, the ninth station, the tenth station, the eleventh station, the twelfth station, the thirteenth station, the fourteenth station, and the first station, and each stays at each station for T seconds to complete three rounds of cleaning; among them, the first station is used to place or take out reaction cups into the reaction cup placement groove, the second station, the sixth station, and the tenth station are used to add cleaning liquid to the reaction cups and shake them evenly, the third station, the seventh station, and the eleventh station are used to collect magnetic beads in the reaction cups in the first stage, the fourth station, the eighth station, and the twelfth station are used to collect magnetic beads in the reaction cups in the second stage, the fifth station, the ninth station, and the thirteenth station are used to extract the waste liquid in the reaction cups, and the fourteenth station is used to add chemiluminescent substrate liquid required for subsequent operations to the reaction cups.

[0014] The present invention has the following beneficial effects compared with the prior art:

[0015] 1. The magnetic separation cleaning device proposed by the present invention optimizes the structural layout and timing sequence. The stations with adjacent timing sequences are separated by four stations, so that the stations with large occupied volumes are dispersed, improving the space utilization rate and facilitating the miniaturization of the device. One sample to be cleaned can be placed every T seconds, and up to 14 samples can be cleaned simultaneously, improving the cleaning sample throughput.

[0016] 2. The magnetic separation cleaning module proposed by the present invention divides the collection of magnetic beads into two stages. In the first stage, a cube magnet with a size of 10mm * 10mm * 16mm is used to gather the magnetic beads at the far end to the near end first. In the second stage, a magnet formed by alternately splicing two cuboid magnets with a size of 5mm * 10mm * 16mm and with N and S poles can gather the magnetic beads at the near end to the reaction cup wall more quickly, improving the speed of collecting magnetic beads and shortening the cleaning time. Moreover, dividing the collection of magnetic beads into two stages shortens the residence time at one station, and to a certain extent, also improves the cleaning sample throughput.

[0017] 3. The magnetic separation cleaning module proposed by the present invention uses two cuboid magnets with a size of 5mm * 10mm * 16mm and with N and S poles alternately spliced at the waste liquid extraction station, which can make the collected magnetic beads agglomerate more tightly, avoid being taken away during waste liquid extraction, and reduce the magnetic bead loss rate. Description of the Drawings

[0018] Figure 1 It is the overall structural layout of an embodiment of the magnetic separation cleaning device proposed by the present invention.

[0019] Figure 2 Schematic diagrams of permanent magnets with three different structures in an embodiment of a magnetic separation and cleaning device proposed by the present invention.

[0020] Figure 3 COMSOL magnetic field force simulation diagrams of permanent magnets with three different structures in an embodiment of a magnetic separation and cleaning device proposed by the present invention.

[0021] In the figure, the reference signs are: first station 1, second station 2, third station 3, fourth station 4, fifth station 5, sixth station 6, seventh station 7, eighth station 8, ninth station 9, tenth station 10, eleventh station 11, twelfth station 12, thirteenth station 13, fourteenth station 14, reaction cup placement groove 101, permanent magnet placement groove 102, first permanent magnet 201, second permanent magnet 202, third permanent magnet 203. Detailed implementation manners

[0022] The present invention will be further described and illustrated below in conjunction with the accompanying drawings and specific implementation manners. The technical features of each implementation manner in the present invention can be combined correspondingly without conflict.

[0023] As Figure 1 shown, a magnetic separation and cleaning device provided by the present invention mainly includes a turntable that can rotate counterclockwise around a central axis. The turntable should be placed horizontally to prevent the reaction liquid from spilling during operation. 14 reaction cup placement grooves 101 are evenly arranged along the circumferential direction on the top surface of the turntable, and the linear distance and the central angle formed between adjacent reaction cup placement grooves 101 are the same. The reaction cup placement groove 101 is used to place the reaction cup containing the magnetic beads to be cleaned and can rotate together with the turntable.

[0024] In the device of the present invention, in the counterclockwise direction, the positions where each reaction cup placement groove 101 is located are sequentially used as the first station 1, the fourth station 4, the seventh station 7, the tenth station 10, the thirteenth station 13, the second station 2, the fifth station 5, the eighth station 8, the eleventh station 11, the fourteenth station 14, the third station 3, the sixth station 6, the ninth station 9, and the twelfth station 12. The positions indicated by each station are fixed and do not change with the rotation of the turntable. That is to say, in the counterclockwise direction, there are 4 stations between adjacent stations in time sequence. This kind of setting can make the stations with large occupied volume be dispersed, improve the space utilization rate, and facilitate the miniaturization of the device.

[0025] In the device of the present invention, permanent magnet placement grooves 102 for placing the first permanent magnet 201 are respectively provided at the reaction cup placement grooves 101 of the third station 3, the seventh station 7, and the eleventh station 11. Permanent magnet placement grooves 102 for placing the second permanent magnet 202 are respectively provided at the reaction cup placement grooves 101 of the fourth station 4, the eighth station 8, and the twelfth station 12. Permanent magnet placement grooves 102 for placing the third permanent magnet 203 are respectively provided at the reaction cup placement grooves 101 of the fifth station 5, the ninth station 9, and the thirteenth station 13. That is to say, there are a total of nine permanent magnet placement grooves 102. The permanent magnet placement grooves 102 and the permanent magnets placed therein do not change their positions with the rotation of the turntable. There is a gap between each group of corresponding permanent magnet placement grooves 102 and reaction cup placement grooves 101 to prevent collision and friction between the reaction cup and the permanent magnet during the rotation of the turntable. However, the gap between the two should not be too large to avoid affecting the working effect of the permanent magnet. When the reaction cup rotates to the corresponding station, it can be directly opposite the magnetic pole surface of the corresponding permanent magnet.

[0026] In this embodiment, the reaction cup placement groove 101 can be set as a circular notch, and the notch size should match the reaction cup; the permanent magnet placement groove 102 can be set as a square notch, and the notch size should match the size of the placed permanent magnet.

[0027] In this embodiment, the first permanent magnet 201, the second permanent magnet 202, and the third permanent magnet 203 can be made of metal alloy materials or ferrite materials. Among them, the metal alloy materials include neodymium iron boron, samarium cobalt, and alnico; the ferrite materials include neodymium iron boron.

[0028] In this embodiment, as Figure 2 (a), the first permanent magnet 201 is a cuboid structure with dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is the surface of 10mm * 16mm, and this surface faces the reaction cup. As Figure 2 (b), the second permanent magnet 202 is composed of two vertically spliced N - pole magnets and S - pole magnets, and the plane where the splicing gap is located passes through the central axis of the turntable. The dimensions of both magnets are 5mm * 10mm * 16mm, and the magnetic pole surfaces are both the surfaces of 5mm * 16mm. After splicing, the obtained second permanent magnet 202 has dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is the surface of 10mm * 16mm. As Figure 2 (c), the third permanent magnet 203 is composed of two horizontally spliced N - pole magnets and S - pole magnets, and the plane where the splicing gap is located is perpendicular to the central axis of the turntable. The dimensions of both magnets are 10mm * 10mm * 8mm, and the magnetic pole surfaces are both the surfaces of 10mm * 8mm. After splicing, the obtained third permanent magnet 203 has dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is the surface of 10mm * 16mm.

[0029] That is to say, during one round of cleaning process, the permanent magnets need to go through three workstations, namely Figure 1 Among them: (1) the workstations for collecting magnetic beads in the first stage with labels 3, 7, and 11; (2) the workstations for collecting magnetic beads in the second stage with labels 4, 8, and 12; (3) the workstations for pumping out waste liquid with labels 5, 9, and 13. Such an arrangement can cooperate with each other to shorten the time for collecting magnetic beads and reduce the loss of magnetic beads. The basis is as follows:

[0030] According to the formula

[0031] F m = μ0KVH grad H

[0032] In the formula, F m —— the magnetic force exerted on the magnetic beads in the magnetic field;

[0033] μ0 —— the magnetic permeability in air;

[0034] K —— the volume magnetization coefficient of the magnetic beads;

[0035] V —— the volume of the magnetic beads;

[0036] H —— the magnetic field strength.

[0037] Among them, μ0 is a constant, and K and V depend on the magnetic beads used. When the magnetic beads used are determined, the values of K and V are fixed values. Therefore, the magnitude of the magnetic force exerted on the magnetic beads at a certain point in the magnetic field is proportional to the magnitude of H grad H at that point, and the direction of the magnetic force exerted is the same as the direction of H grad H.

[0038] Using COMSOL software, simulate the three-dimensional magnetic field distribution of the magnets used, calculate the magnitude and direction of H grad H at each point in the key cross-section and represent them with surface arrows.

[0039] Analysis Figure 2 the cross-sections shown as a and b in Figure 3 Among them, (a) and (a1) in Figure 3(b) and (b1) are simulation results of a 10mm*10mm*16mm cube magnet. The size of the arrow represents the magnitude of the magnetic field force at that point (due to the large difference in the magnitude of the magnetic field force near and far from the magnet, logarithmic processing is used here for ease of observation), and the direction represents the direction of the magnetic field force at that point. It can be clearly seen that at the near end of the magnet, the magnetic field force generated by the magnet formed by alternating N and S poles is stronger than that of the cube magnet, while at the far end of the magnet, the magnetic field force generated by the magnet formed by alternating N and S poles is weaker than that of the cube magnet. Because the magnetic beads are evenly dispersed in the reaction cup before they are collected, placing a 10mm*10mm*16mm cube magnet at the position where the magnetic beads are collected in the first stage can better collect the magnetic beads at the far end. After the first stage of collecting the magnetic beads, the magnetic beads are gathered at the proximal end of the magnet. Then, placing two 5mm*10mm*16mm rectangular magnets with alternating N and S poles at the position where the magnetic beads are collected in the second stage can take advantage of the strong proximal magnetic field force and increase the speed of collecting magnetic beads. After two stages of collecting the magnetic beads, the magnetic beads are gathered and adsorbed on the wall of the reaction cup. At the position where the waste liquid is extracted, the magnetic field force is needed to restrain the magnetic beads to prevent them from being carried away with the waste liquid. Analysis Figure 2 The vertical magnetic field force component of the middle b section under the magnetic field distribution of the second permanent magnet 202 and the third permanent magnet 203 is: Figure 3 (c) in FIG. 2 corresponds to the second permanent magnet 202. Figure 3 (c1) corresponds to the third permanent magnet 203. The second permanent magnet 202 has a stronger magnetic field force gathering toward the middle at the edge, while the magnetic field force gathering in the vertical direction near the middle is very weak. The magnetic beads gathered on the wall of the reaction cup will appear in strips, while the third permanent magnet 203 has a stronger magnetic field force gathering toward the middle near the middle, which will make the strip-shaped magnetic beads gather into dots. The magnetic beads are gathered more tightly, effectively preventing the magnetic beads from being carried away when the waste liquid is extracted, thereby reducing the loss of magnetic beads.

[0040] The cleaning method using the magnetic separation cleaning device is as follows:

[0041] The turntable rotates counterclockwise once every T seconds and rotates through five workstations each time. Reaction cups are placed in sequence, so that the reaction cups stay at each workstation for T seconds in the order of the first workstation 1, the second workstation 2, the third workstation 3, the fourth workstation 4, the fifth workstation 5, the sixth workstation 6, the seventh workstation 7, the eighth workstation 8, the ninth workstation 9, the tenth workstation 10, the eleventh workstation 11, the twelfth workstation 12, the thirteenth workstation 13, the fourteenth workstation 14, and then back to the first workstation 1, completing three rounds of cleaning. Among them, the first workstation 1 is used to place or remove reaction cups into or from the reaction cup placement groove 101. The second workstation 2, the sixth workstation 6, and the tenth workstation 10 are used to add cleaning liquid to the reaction cups and shake them evenly. The third workstation 3, the seventh workstation 7, and the eleventh workstation 11 are used to collect magnetic beads in the reaction cups in the first stage. The fourth workstation 4, the eighth workstation 8, and the twelfth workstation 12 are used to collect magnetic beads in the reaction cups in the second stage. The fifth workstation 5, the ninth workstation 9, and the thirteenth workstation 13 are used to extract waste liquid from the reaction cups. The fourteenth workstation 14 is used to add chemiluminescent substrate liquid required for subsequent operations to the reaction cups.

[0042] That is to say, one round of cleaning the reaction cups will be carried out through four workstations respectively: 1. Add cleaning liquid and shake evenly; 2. Collect magnetic beads in the first stage; 3. Collect magnetic beads in the second stage; 4. Extract waste liquid. Specifically, the reaction cup is placed from the first workstation 1, rotated to the second workstation 2 to add cleaning liquid and shake evenly, then rotated to the third workstation 3 to collect magnetic beads in the first stage, then rotated to the fourth workstation 4 to collect magnetic beads in the second stage, and then rotated to the fifth workstation 5 to extract waste liquid, thus completing one round of cleaning. And so on, after three rounds of cleaning, finally rotate to the first workstation 1 and take out the reaction cup from the first workstation 1. During this process, when one reaction cup rotates from the first workstation 1 to the second workstation 2, the second reaction cup can be placed in the reaction cup placement groove at the first workstation 1. And so on, up to 14 samples can be cleaned simultaneously at most. In the case of three rounds of cleaning, it takes 14*T seconds for the first sample to complete cleaning, and the time for each subsequent sample to complete cleaning is only T seconds different from the previous sample, greatly improving the throughput of cleaning samples.

[0043] In the device of the present invention, permanent magnets with different structures are arranged and cooperate with each other at the workstations for collecting magnetic beads in the first stage, collecting magnetic beads in the second stage, and extracting waste liquid, shortening the time for collecting magnetic beads and reducing the magnetic bead loss rate at the same time. The device optimizes the working timing. One sample to be cleaned can be placed every T seconds. In the case of three rounds of cleaning, it takes 14*T seconds for the first sample to complete cleaning, and the time for each subsequent sample to complete cleaning is only T seconds different from the previous sample, greatly improving the throughput of cleaning samples.

[0044] The embodiments described above are only a preferred solution of the present invention, but they are not intended to limit the present invention. Those of ordinary skill in the relevant technical fields can still make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all technical solutions obtained by means of equivalent substitution or equivalent transformation fall within the protection scope of the present invention.

Claims

1. A magnetic separation cleaning device, characterized in that, It includes a turntable that can rotate counterclockwise around the central axis; on the top surface of the turntable, 14 reaction cup placement slots (101) that can rotate with the turntable are evenly arranged in the circumferential direction. The reaction cup placement slots (101) are used to place reaction cups containing magnetic beads to be cleaned; in the counterclockwise direction, the positions where each reaction cup placement slot (101) is located are successively used as the first station (1), the fourth station (4), the seventh station (7), the tenth station (10), the thirteenth station (13), the second station (2), the fifth station (5), the eighth station (8), the eleventh station (11), the fourteenth station (14), the third station (3), the sixth station (6), the ninth station (9), and the twelfth station (12). The positions indicated by each station are fixed and do not change with the rotation of the turntable; at the reaction cup placement slots (101) located at the third station (3), the seventh station (7), and the eleventh station (11), permanent magnet placement slots (102) for placing the first permanent magnet (201) are respectively provided. At the reaction cup placement slots (101) located at the fourth station (4), the eighth station (8), and the twelfth station (12), permanent magnet placement slots (102) for placing the second permanent magnet (202) are respectively provided. At the reaction cup placement slots (101) located at the fifth station (5), the ninth station (9), and the thirteenth station (13), permanent magnet placement slots (102) for placing the third permanent magnet (203) are respectively provided; the permanent magnet placement slots (102) do not change their positions with the rotation of the turntable; there is a gap between each group of corresponding permanent magnet placement slots (102) and reaction cup placement slots (101), and when the reaction cup rotates to the corresponding station, it can be directly opposite to the magnetic pole surface of the permanent magnet; The first permanent magnet (201) is a cuboid structure with dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is a surface of 10mm * 16mm; The second permanent magnet (202) is composed of two vertically spliced N - pole magnets and S - pole magnets. The plane where the splicing gap is located passes through the central axis of the turntable; the dimensions of both magnets are 5mm * 10mm * 16mm, and the magnetic pole surfaces are both surfaces of 5mm * 16mm. After splicing, the obtained second permanent magnet (202) has dimensions of 10mm * 10mm * 16mm, and the magnetic pole surface is a surface of 10mm * 16mm; The first station (1) is used to put into or take out reaction cups from the reaction cup placement slots (101). The second station (2), the sixth station (6), and the tenth station (10) are used to add cleaning liquid to the reaction cups and shake them evenly. The third station (3), the seventh station (7), and the eleventh station (11) are used to collect the magnetic beads in the reaction cups in the first stage. The fourth station (4), the eighth station (8), and the twelfth station (12) are used to collect the magnetic beads in the reaction cups in the second stage. The fifth station (5), the ninth station (9), and the thirteenth station (13) are used to extract the waste liquid in the reaction cups. The fourteenth station (14) is used to add the chemiluminescence substrate solution required for subsequent operations to the reaction cups.

2. The magnetic separation and cleaning device according to claim 1, characterized in that, The reaction cup placement groove (101) is a circular notch, and the permanent magnet placement groove (102) is a square notch.

3. The magnetic separation and cleaning device according to claim 1, characterized in that, The first permanent magnet (201), the second permanent magnet (202), and the third permanent magnet (203) are made of metal alloy material or ferrite material.

4. A magnetic separation cleaning device according to claim 1, characterized in that, The third permanent magnet (203) is composed of two N-pole magnets and S-pole magnets spliced horizontally. The plane where the splicing gap is located is perpendicular to the central axis of the turntable; the size of each of the two magnets is 10mm * 10mm * 8mm, and the magnetic pole surface is a 10mm * 8mm surface. After splicing, the size of the obtained third permanent magnet (203) is 10mm * 10mm * 16mm, and the magnetic pole surface is a 10mm * 16mm surface.

5. A cleaning method using the magnetic separation cleaning device according to any one of claims 1 to 4, characterized in that, Specifically as follows: The turntable rotates counterclockwise once every T seconds and rotates five workstations each time. It sequentially places reaction cups, so that the reaction cups are in the order of the first workstation (1), the second workstation (2), the third workstation (3), the fourth workstation (4), the fifth workstation (5), the sixth workstation (6), the seventh workstation (7), the eighth workstation (8), the ninth workstation (9), the tenth workstation (10), the eleventh workstation (11), the twelfth workstation (12), the thirteenth workstation (13), and the fourteenth workstation (14), and the first workstation (1). Each reaction cup stays at each workstation for T seconds to complete three rounds of cleaning; among them, the first workstation (1) is used to put into or take out reaction cups from the reaction cup placement groove (101), the second workstation (2), the sixth workstation (6), and the tenth workstation (10) are used to add cleaning liquid to the reaction cups and shake them evenly, the third workstation (3), the seventh workstation (7), and the eleventh workstation (11) are used to collect magnetic beads in the reaction cups in the first stage, the fourth workstation (4), the eighth workstation (8), and the twelfth workstation (12) are used to collect magnetic beads in the reaction cups in the second stage, the fifth workstation (5), the ninth workstation (9), and the thirteenth workstation (13) are used to extract waste liquid from the reaction cups, and the fourteenth workstation (14) is used to add chemiluminescent substrate liquid required for subsequent operations to the reaction cups.

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