A method for preparing carboxylated porous microspheres
By preparing porous microspheres through emulsification and adding a carboxyl crosslinking agent during stirring, the problem of insufficient stability of carboxylated porous microspheres was solved, achieving efficient nucleic acid extraction and purification.
CN117225318BActive Publication Date: 2026-05-08XIAMEN WIZ BIOTECH CO LTD
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202311251428.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-26
- Publication Date
- 2026-05-08
- Estimated Expiration
- 2043-09-26
AI Technical Summary
Technical Problem
Existing carboxylated porous microspheres are not stable enough during nucleic acid extraction and purification, resulting in poor application effects.
Method used
Porous microspheres were prepared by emulsification. The porous structure was formed by evaporating organic solvents during stirring, and a carboxyl crosslinking agent was added during stirring to form a semi-interpenetrating polymer network structure, thereby improving the stability and carboxyl distribution of the microspheres.
Benefits of technology
This improved the stability of porous microspheres and the efficiency of nucleic acid extraction and purification, ensuring uniform contact and efficient separation with nucleic acids.
✦ Generated by Eureka AI based on patent content.
Abstract
The application relates to the technical field of microspheres, and particularly provides a preparation method of carboxylated porous microspheres, which comprises the following steps: adding a biocompatible polymer, an acrylic copolymer and an oil-soluble emulsifier into an organic solvent to be dissolved and prepared into a dispersed phase; adding a water-soluble emulsifier and / or a protective colloid into water to be prepared into a continuous phase; mixing the dispersed phase and the continuous phase to obtain an emulsion, continuously stirring until precipitation appears, collecting the precipitation, cleaning, drying and obtaining the carboxylated porous microspheres. In the continuous stirring stage, air blowing or a crosslinking agent can be added. The carboxylated porous microspheres have good fluidity and good nucleic acid extraction and purification effects.
Need to check novelty before this filing date? Find Prior Art