Acidic phyllostachys zhejiangensis planting soil and preparation method thereof

Abstract

The application provides a Zhejiang phoebe acid planting soil and a preparation method thereof, and belongs to the technical field of planting soil. The Zhejiang phoebe acid planting soil is composed of vinegar residue, coconut husk, fruit shell, acid-modified wet garbage, acid-modified garden waste, garden soil and fine-toothed acaulospora. The application also provides a preparation method of the Zhejiang phoebe acid planting soil, which comprises the preparation of acid-modified wet garbage, the preparation of acid-modified garden waste, the extraction of acaulospora spores, the mixing of vinegar residue, coconut husk, fruit shell, acid-modified wet garbage, acid-modified garden waste, garden soil and acaulospora spores according to the weight ratio, and the preparation of the Zhejiang phoebe acid planting soil. The Zhejiang phoebe acid planting soil provided by the application can increase the survival rate of Zhejiang phoebe to 98%, and provides a powerful guarantee for the introduction of urban acid plants and the biodiversity, and provides an effective way for the resource utilization of urban organic waste.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of planting soil, and particularly to Phoebe chekiangensis acid planting soil and a preparation method thereof. BACKGROUND

[0002] Phoebe chekiangensis C.B.Shang belongs to Lauraceae and Phoebe, and is a new species discovered by Professor Xiang Bo, a famous plant taxonomist in China, on Tiamoshan Mountain and Longtang in Zhejiang Province in the 1960s. The average annual temperature of the distribution area of Phoebe chekiangensis is 16-18℃, the extreme minimum temperature is-5 to-9.6℃, and the extreme maximum temperature is 38℃. At present, Phoebe chekiangensis is distributed in Zhejiang Province, Fujian Province, Jiangxi Province and the southern part of Anhui Province in China, and is often distributed in forest land or valley land with an altitude of less than 1000m, and is suitable for living in warm and humid climate and acid or slightly acid soil.

[0003] With the vigorous development of urban ecological greening construction, urban plant diversity has gradually been valued, and the cultivation of new varieties and the introduction of foreign tree species are important technical means to beautify the city. Phoebe chekiangensis is a evergreen broad-leaved tall tree, which is high and dignified, magnificent and luxuriant in branches and leaves, and is an ideal street tree and landscape tree. The tall and straight posture of Phoebe chekiangensis is a beautiful scenery line of urban greening.

[0004] Phoebe chekiangensis mainly grows in acid soil environment, but part of the urban soil is alkaline soil, which is not suitable for the survival and growth of Phoebe chekiangensis. The existing methods for changing the acid of urban landscaping soil include adding peat, chemical fertilizer (ammonium sulfate, calcium phosphate, ammonium nitrate, ferrous sulfate, calcium sulfate), wood vinegar and humic acid. Peat (grass carbon) is a limited natural resource, and with blind exploitation, peat resources are gradually exhausted; chemical fertilizer can quickly reduce the acid-base value of the material, but it is easy to lose, has poor durability, has a great influence on soil structure, and has a strong damage to soil structure; wood vinegar is a by-product under high temperature, which is rich in acetic acid, phenols, potassium, calcium, magnesium, zinc and other minerals, has a simple molecular structure and is easy to lose; humic acid is a kind of organic matter caused and accumulated by a series of processes of microbial decomposition and transformation of animal and plant remains (mainly plant remains) and geochemistry, and China is rich in humic acid resources, with large reserves, wide distribution and good grade. As an organic material, humic acid is widely used in agriculture.

[0005] The application discloses an agricultural soil improver for improving soil structure, and is composed of the following components in parts by weight: alkali slag 20-26 parts, municipal waste 10-20 parts, amitraz 15-22 parts, riverbed black mud 10-18 parts, wood vinegar 9-14 parts, nitro humic acid 6-12 parts, tannic acid 8-12 parts, calcium hydrogen phosphate 10-18 parts, phosphorite gypsum 6-9 parts, bone charcoal 8-13 parts, spirulina powder 12-16 parts, dolomite powder 16-19 parts, ferrous sulfate 7-10 parts, straw crushed material 12-22 parts, calcium nitrate 5-12 parts, silicone rubber 3-7 parts, lignin sulfonate 2-6 parts and microbial inoculum 1-4 parts.

[0006] On the other hand, the resources of organic waste in cities are relatively rich, and it is difficult to solve the problem of how to use local materials, to improve the soil structure, to adjust the pH value of the soil, to improve the soil gelation rate, to prevent the soil from being hardened, and to solve the problems of fast water evaporation, lack of organic matter and weak soil fertility in the land by screening and improving the organic waste in cities and applying the organic waste generated in cities in the planting soil of Phoebe zhennan. SUMMARY

[0007] The application provides an acid planting soil for Phoebe zhennan, which is suitable for the introduction and cultivation of Phoebe zhennan and has a stable and durable soil structure, improves the reutilization rate of the organic waste generated in cities, provides a technical reference for other acid plants and flowers, and solves the technical problem that the city soil in the prior art is not suitable for the planting and growth of acid plants.

[0008] The acid planting soil for Phoebe zhennan comprises the following raw materials in parts by weight:

[0009] vinegar residue 0-10 parts;

[0010] coconut husk 0-10 parts;

[0011] fruit shell 0-10 parts;

[0012] acid-modified wet garbage 5-20 parts;

[0013] acid-modified garden waste 5-20 parts;

[0014] garden soil 50-75 parts;

[0015] Acaulospora denticulata 0.05-0.1 parts.

[0016] Preferably, the vinegar residue is washed with water for 1-2 times before use.

[0017] Preferably, the coconut shell is washed with water for 1-2 times before use.

[0018] Because the vinegar residue and the coconut shell contain certain salt, which will affect the growth of Phoebe chekiangensis, the salt in the vinegar residue and the coconut shell is dissolved by water, and then removed.

[0019] The shell includes thick-walled fruit shells such as hickory shells.

[0020] Preferably, the wet garbage includes fermentation products of vegetables and fruit peels, and fermentation products of non-food waste.

[0021] Preferably, the acid-modified wet garbage is prepared by composting humic acid and the wet garbage.

[0022] Further preferably, the weight ratio of the wet garbage to the humic acid is 4.5-19:1.

[0023] Preferably, the garden waste includes dry branches and fallen leaves.

[0024] Preferably, the acid-modified garden waste is prepared by composting humic acid and the garden waste.

[0025] Further preferably, the weight ratio of the garden waste to the humic acid is 4.5-19:1.

[0026] The wet garbage and the garden waste are alkaline improvement materials, in order to apply the wet garbage and the garden waste to the planting soil of Phoebe chekiangensis, the wet garbage and the garden waste are acid-modified by humic acid. Humic acid is an organic polymer compound extracted from natural coal or artificially synthesized, and contains complex organic groups. The humic acid can establish a close relationship with humus in the wet garbage and the garden waste through biochemical reactions, thereby effectively and durably maintaining the acidic environment of the wet garbage and the garden waste, and further durably maintaining the structure stability of the planting soil, thereby laying a foundation for the preparation of the acidic planting soil for the growth of Phoebe chekiangensis.

[0027] Preferably, after the wet garbage and the humic acid are mixed and composted, the water content of the compost is 30%-40%.

[0028] Preferably, after the garden waste and the humic acid are mixed and composted, the water content of the compost is 30%-40%.

[0029] The appropriate water content can maintain the slow dissolution of the humic acid and promote the biochemical reactions between the humic acid and the wet garbage and the garden waste.

[0030] The garden soil is the soil of a planting field, which can make the Phoebe zheliensis adapt to the soil environment and the microbial environment of the planting field more quickly.

[0031] The Glomus etunicatum is a mycorrhizal fungus symbiotic with the Phoebe zheliensis, which can promote the growth of the Phoebe zheliensis and prevent diseases.

[0032] Preferably, the acidic planting soil for the growth of the Phoebe zheliensis comprises the following raw materials in parts by weight:

[0033] Vinegar residue 5-10 parts;

[0034] Coconut husk 5-10 parts;

[0035] Shell 5-10 parts;

[0036] Acid-modified wet garbage 5-20 parts;

[0037] Acid-modified garden waste 5-20 parts;

[0038] Garden soil 50-75 parts;

[0039] Glomus etunicatum 0.05-0.1 parts.

[0040] The application further provides a preparation method of the acidic planting soil for the Phoebe zheliensis.

[0041] The preparation method of the acidic planting soil for the Phoebe zheliensis comprises the following steps:

[0042] Preparation of the acid-modified wet garbage: wet garbage and humic acid are weighed according to a weight ratio of 4.5-19:1, mixed, the water content of the heap is adjusted to 30%-40%, and the acid-modified wet garbage is prepared after stacking for 5-7 days and turning over 1-2 times;

[0043] Preparation of the acid-modified garden waste: garden waste and humic acid are weighed according to a weight ratio of 4.5-19:1, mixed, the water content of the heap is adjusted to 30%-40%, and the acid-modified garden waste is prepared after stacking for 5-7 days and turning over 1-2 times;

[0044] Extraction of spores of the Glomus etunicatum:

[0045] S1, rhizosphere soil of healthy seedlings of the Phoebe zheliensis is dissolved in distilled water to prepare a mixed solution by stirring;

[0046] S2, the mixed solution is passed through a sieve with a mesh number of 300, and the filtrate is collected in a centrifuge tube;

[0047] S3, the centrifuge tube in step S2 is centrifuged at 3000 r / min for 5 min in a centrifuge, and the supernatant is discarded;

[0048] S4, adding 70% sucrose solution into the centrifugal tube in step S3, centrifuging in the centrifuge under the condition of 3000 r / min for 1 min, and taking out the supernatant;

[0049] S5, collecting the filtrate of the supernatant prepared in step S4 in a culture dish through a 300-mesh sieve;

[0050] S6, washing the filtrate in the culture dish in step S5 with distilled water, and observing and collecting the granular ornament with yellow-brown annular protrusions on the surface, smooth edges, and dark brown 120-180 μm size of the mycelium under a stereomicroscope, which is the Absidia corymbifera spore.

[0051] The vinegar residue, coconut husk, fruit shell, acid-modified wet garbage, acid-modified garden waste, garden soil and Absidia corymbifera spore are weighed and mixed uniformly to prepare the Zhejiang nan acid planting soil.

[0052] Preferably, in the process of extracting the Absidia corymbifera spore, the Zhejiang nan healthy seedlings are 3-5 years old Zhejiang nan healthy seedlings.

[0053] Preferably, in the process of extracting the Absidia corymbifera spore, the mixed solution is first passed through a 20-mesh sieve before passing through a 300-mesh sieve to remove large-particle impurities and facilitate the purification and extraction of the Absidia corymbifera spore.

[0054] To enable the Absidia corymbifera spore in the soil to be fully extracted, preferably, in the process of extracting the Absidia corymbifera spore, the operation steps S4 to S5 are repeated 1-3 times.

[0055] Compared with the prior art, the present application has the following advantages:

[0056] (1) Compared with the planting soil prepared by directly mixing all raw materials, the survival rate of Zhejiang nan in the planting soil prepared by the method of the Zhejiang nan acid planting soil provided by the present application can reach 98%, while the survival rate of Zhejiang nan in the planting soil prepared by directly mixing all raw materials is only 5%.

[0057] (2) The Zhejiang nan acid planting soil provided by the present application mainly selects organic waste generated in the city and garden soil as raw materials, improving the resource utilization rate of organic waste generated in the city.

[0058] (3) The Zhejiang nan acid planting soil provided by the present application utilizes the interaction of humic acid with humus in wet garbage and forest and garden waste to durably and stably structure the planting soil, ensuring high survival rate of Zhejiang nan.

[0059] (4) City garden flowers and many important tree species are acidophilic environment, the development of Zhejiang Phoebe acid planting soil provides a strong guarantee for the introduction of urban plants and biodiversity, while providing an effective way for the resource utilization of urban organic waste. DETAILED DESCRIPTION

[0060] The technical solutions of the present application are further described and illustrated by the following examples. The raw materials used in the examples are commercially available or prepared by conventional methods.

[0061] Example 1

[0062] A Zhejiang Phoebe acid planting soil is composed of the following raw materials by weight:

[0063] Vinegar residue 10 parts;

[0064] Coconut husk 10 parts;

[0065] Walnut shell 10 parts;

[0066] Acid-modified wet garbage 10 parts;

[0067] Acid-modified garden waste 10 parts;

[0068] Garden soil 50 parts;

[0069] Blastocladia microti 0.1 parts.

[0070] The preparation method of the acid planting soil for the growth of Zhejiang Phoebe is as follows:

[0071] Preparation of acid-modified wet garbage: wet garbage and humic acid are weighed according to the weight ratio of 19:1, mixed, the moisture content of the pile is adjusted to 40%, and the acid-modified wet garbage is prepared after stacking for 5 days with intermediate turning once;

[0072] Preparation of acid-modified forest garden waste: garden waste and humic acid are weighed according to the weight ratio of 19:1, mixed, the moisture content of the pile is adjusted to 30%, and the acid-modified garden waste is prepared after stacking for 5 days with intermediate turning once;

[0073] Extraction of Blastocladia microti spores:

[0074] S1, 50g of rhizosphere soil of 3-5 year old Zhejiang Phoebe healthy seedlings is dissolved in 250ml of distilled water, and a mixed solution is prepared by stirring;

[0075] S2, the mixed solution is passed through two layers of screens, the upper layer of screen has a mesh size of 20 meshes, and the lower layer of screen has a mesh size of 300 meshes, and the filtrate is collected in a 100ml centrifuge tube after washing with distilled water for a few seconds;

[0076] S3, centrifuge the centrifuge tube in step S2 in the centrifuge at 3000 r / min for 5 min, let the Blastocladia pringsheim spores precipitate, discard the supernatant;

[0077] S4, add 70% sucrose solution to the centrifuge tube in step S3, centrifuge in the centrifuge at 3000 r / min for 1 min, take out the supernatant;

[0078] S5, collect the filtrate of the supernatant prepared in step S4 through a 300 mesh sieve in a culture dish;

[0079] S6, repeat steps S4 to S5;

[0080] S7, rinse the filtrate in the culture dish in step S6 with distilled water, wash away the sucrose on the surface of the Blastocladia pringsheim spores, then observe and collect the granular ornamentation with yellow-brown annular protrusions on the surface under a stereomicroscope, the 120-180 μm sized bacteria with smooth edges and dark brown color are the Blastocladia pringsheim spores;

[0081] Wash the vinegar residue and coconut shells with clean water for 2 times;

[0082] Mix the washed vinegar residue, washed coconut shells, walnut shells, the prepared acid-modified wet garbage, the prepared acid-modified garden waste, garden soil and extracted Blastocladia pringsheim spores in equal weight parts to obtain an acid planting soil for the growth of Zhejiang nan, numbered as A01.

[0083] Detect the pH value of the acid planting soil for the growth of Zhejiang nan using a pH meter, randomly detect 5 points, and the test results are shown in Table 1.

[0084] Table 1 pH value of the acid planting soil for the growth of Zhejiang nan prepared in Example 1

[0085]

[0086] Example 2

[0087] An acid planting soil for the growth of Zhejiang nan is composed of the following raw materials by weight:

[0088] Vinegar residue 0 parts;

[0089] Coconut shells 0 parts;

[0090] Walnut shells 0 parts;

[0091] Acid-modified wet garbage 20 parts;

[0092] Acid-modified garden waste 20 parts;

[0093] Garden soil 75 parts;

[0094] Absidia corymbifera var. gamsii 0.1 parts.

[0095] The preparation method of the acidic planting soil for the growth of Phoebe chekiangensis is as follows:

[0096] Preparation of the acid-modified wet garbage: the wet garbage and humic acid are weighed according to the weight ratio of 19:1, mixed, the moisture content of the pile is adjusted to 40%, and the acid-modified wet garbage is prepared after 7 days of stacking with 2 times of intermediate turning;

[0097] Preparation of the acid-modified garden waste: the garden waste and humic acid are weighed according to the weight ratio of 4.5:1, mixed, the moisture content of the pile is adjusted to 30%, and the acid-modified garden waste is prepared after 7 days of stacking with 2 times of intermediate turning;

[0098] Extraction of Absidia corymbifera var. gamsii spores: the extraction steps of the Absidia corymbifera var. gamsii spores are the same as those in the extraction steps of the Absidia corymbifera var. gamsii spores in Example 1;

[0099] The vinegar residue and coconut shells are washed with clean water for 2 times;

[0100] The washed vinegar residue, the washed coconut shells, the acid-modified wet garbage prepared above, the acid-modified garden waste prepared above, the garden soil, and the extracted Absidia corymbifera var. gamsii spores are weighed according to the weight parts, and are uniformly mixed to obtain the acidic planting soil for the growth of Phoebe chekiangensis, numbered A02.

[0101] The pH value of the acidic planting soil for the growth of Phoebe chekiangensis is detected by using a pH meter, 5 points are randomly detected, and the test results are shown in Table 2.

[0102] Table 2 pH value of the acidic planting soil for the growth of Phoebe chekiangensis prepared in Example 2

[0103]

[0104] Example 3

[0105] An acidic planting soil for the growth of Phoebe chekiangensis is composed of the following raw materials by weight parts:

[0106] Vinegar residue 5 parts;

[0107] Coconut shells 5 parts;

[0108] Walnut shells 5 parts;

[0109] Acid-modified wet garbage 15 parts;

[0110] Acid-modified garden waste 15 parts;

[0111] Garden soil 50 parts;

[0112] Absidia corymbifera var. gamsii 0.05 parts.

[0113] The preparation method of the acidic planting soil for the growth of Phoebe chekiangensis is as follows:

[0114] Preparation of the acid-modified wet garbage: the wet garbage and humic acid are weighed according to the weight ratio of 4.5:1, mixed, the water content of the pile is adjusted to 35%, and the acid-modified wet garbage is prepared after 6 days of stacking with 2 times of intermediate turning;

[0115] Preparation of the acid-modified garden waste: the garden waste and humic acid are weighed according to the weight ratio of 19:1, mixed, the water content of the pile is adjusted to 35%, and the acid-modified garden waste is prepared after 6 days of stacking with 2 times of intermediate turning;

[0116] Extraction of Blastocladia granulata spores: the extraction steps of Blastocladia granulata spores are the same as those in Example 1.

[0117] The vinegar residue and coconut shells are washed with clean water for 2 times;

[0118] The washed vinegar residue, washed coconut shells, walnut shells, acid-modified wet garbage prepared above, acid-modified garden waste prepared above, garden soil, and extracted Blastocladia granulata spores are weighed according to the weight parts, mixed uniformly, and the acidic planting soil for the growth of Phoebe chekiangensis is obtained, labeled as A03.

[0119] The pH value of the acidic planting soil for the growth of Phoebe chekiangensis is detected by using an acidity meter, 5 points are randomly detected, and the test results are shown in Table 3.

[0120] Table 3 pH value of the acidic planting soil for the growth of Phoebe chekiangensis prepared in Example 3

[0121]

[0122] Comparative Example 1

[0123] An acidic planting soil is prepared from the following raw materials by weight parts:

[0124] Vinegar residue 10 parts;

[0125] Coconut shells 10 parts;

[0126] Walnut shells 10 parts;

[0127] Garden soil 50-75 parts;

[0128] Wet garbage 20 parts;

[0129] Garden waste 20 parts;

[0130] Blastocladia granulata 0.1 part.

[0131] Humic acid 1 part

[0132] The preparation method of the planting soil is as follows:

[0133] The vinegar residue, coconut husk, walnut shell, garden soil, wet garbage, garden waste, fine-toothed acellular cystospore and humic acid are weighed by parts by weight, and uniformly mixed to obtain the planting soil numbered B01.

[0134] Transplanting of Phoebe zhennan

[0135] 30 three-year-old seedlings of Phoebe zhennan are transplanted in the acidic planting soil for the growth of Phoebe zhennan prepared in Example 1, Example 2 and Example 3 respectively, and 30 three-year-old seedlings of Phoebe zhennan are planted in the planting soil prepared in Proportion 1, and grown in the same environment for one year. The growth conditions of Phoebe zhennan after one year are shown in Table 4.

[0136] Table 4 Influence of different types of planting soil on the growth of Phoebe zhennan

[0137]

[0138] As shown in Table 4, the survival rate of Phoebe zhennan planted in the acidic planting soil for Phoebe zhennan provided by the application can reach 98%, while the survival rate of Phoebe zhennan planted in the planting soil prepared in Comparative Example 1 is only 5%.

[0139] This is because the wet garbage and garden waste in the planting soil provided by the application are all after composting, and do not need to be composted again, and after acidification by humic acid, the structure is stable, so the structure of the planting soil is stable and the nutrition is sufficient.

[0140] However, the planting soil prepared in Comparative Example 1 is directly mixed with all raw materials, so the humic acid not only acts on the wet garbage and garden waste, but also acts on other components in the planting soil, which will reduce the concentration of humic acid and reduce the biochemical reaction efficiency between humic acid, wet garbage and garden waste; and when the humic acid acts on the wet garbage and garden waste, it will constantly change the structure and environment of the planting soil, and in addition, the wet garbage and garden waste cannot be completely acidified by the humic acid due to the waste of humic acid, which further makes the structure of the planting soil unstable; in addition, although directly mixing all raw materials can weaken the alkaline environment of the garden soil, the pH value is reduced from 8.1 to 7.8, but the planting soil is still alkaline, which is not conducive to the survival of the strain and the survival of Phoebe zhennan, and finally leads to low survival rate of Phoebe zhennan and cannot adapt to the growth of Phoebe zhennan.

[0141] It should be understood that the above examples are only used to illustrate the content of the present application and should not be considered as limitation to the present application. The examples in the present application and the features in the examples can be combined with each other arbitrarily without conflict. The protection scope of the present application should be the technical solutions recited in the claims and the equivalent replacement solutions of the technical features recited in the claims. That is, the equivalent replacement improvement within the scope should also be within the protection scope of the present application.

Claims

1. A method for preparing acidic planting soil for *Phoebe zhennan* trees in Zhejiang Province, characterized in that, Includes the following steps: Preparation of modified acid wet waste: Weigh wet waste and humic acid according to a weight ratio of 4.5 to 19:1, mix them, adjust the moisture content of the pile to 30% to 40%, pile for 5 to 7 days, turning the pile 1 to 2 times in between to obtain modified acid wet waste. Preparation of modified humic waste: Weigh humic waste and humic acid according to a weight ratio of 4.5 to 19:

1. After mixing, adjust the moisture content of the pile to 30% to 40%. Pile for 5 to 7 days, turning the pile 1 to 2 times in between to obtain modified humic waste. Extraction of spores of *Cytomyces dentata*: S1. Take the rhizosphere soil of healthy young seedlings of Nanmu (Phoebe zhennan) from Zhejiang, dissolve it in distilled water, and stir to prepare a mixture; S2. Pass the mixture through a 300-mesh sieve and collect the filter material in a centrifuge tube; S3. Centrifuge the centrifuge tubes from step S2 in a centrifuge at 3000 r / min for 5 min and discard the supernatant. S4. Add 70% sucrose solution to the centrifuge tube in step S3, centrifuge at 3000 r / min for 1 min, and take out the supernatant. S5. Pass the supernatant obtained in step S4 through a 300-mesh sieve and collect the filtrate in a petri dish; S6. Rinse the culture dish filtered in step S5 with distilled water, and observe and collect the granular ornamentation with yellow-brown annular protrusions on the surface, smooth edges, dark brown, and 120-180 μm in size under a stereomicroscope. These are the spores of *Aphelenchus dentata*. Weigh out 0-10 parts of vinegar residue, 0-10 parts of coconut coir, 0-10 parts of fruit shells, 5-20 parts of acid-modified wet waste, 5-20 parts of acid-modified garden waste, 50-75 parts of garden soil, and 0.05-0.1 parts of fine-toothed sessile spores according to the specified weight ratios, mix them evenly, and prepare Zhejiang Nan acidic planting soil.

2. The method for preparing acidic planting soil for *Phoebe zhennan* plants according to claim 1, characterized in that, The vinegar residue should be rinsed with clean water 1-2 times before use; the coconut coir should be rinsed with clean water 1-2 times before use.

3. The method for preparing acidic planting soil for *Machilus nanmu* according to claim 1, characterized in that, The wet waste includes fermented products from vegetables and fruit peels, as well as fermented products from non-food waste.

4. The method for preparing acidic planting soil for *Phoebe zhennan* plants according to claim 1, characterized in that, After the wet waste and humic acid are mixed and composted, the moisture content of the compost pile is 30% to 40%.

5. The method for preparing acidic planting soil for *Phoebe zhennan* plants according to claim 1, characterized in that, The garden waste includes dead branches and fallen leaves.

6. The method for preparing acidic planting soil for *Machilus nanmu* according to claim 1, characterized in that, The moisture content of the compost pile after the garden waste and humic acid are mixed is 30% to 40%.

7. The method for preparing the acidic planting soil for *Phoebe zhennan* according to claim 1, characterized in that, During the extraction of spores of *Aphelenchoides dentata*, steps S4 and S5 were repeated 1 to 3 times.

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