Whitening anti-wrinkle composition and method of preparation and eye cream thereof

Abstract

The application discloses a whitening and anti-wrinkle composition and a preparation method and an eye cream thereof, the eye cream contains the following raw materials: plant extract, petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, camosine, octyldodecanol lauroyl glutamate, caffeine, vitamin E, carbomer, butylated hydroxytoluene, palmitoyl tripeptide-1 and water. The plant extract contains sapindus peruviana peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, curcuma longa rhizome extract, geranium maculatum extract, citrus aurantium dulcis seed extract and cinnamomum cassia bark extract. The application selects natural effective components in plants, camosine, vitamin E and palmitoyl tripeptide-1 and other substances reasonably, controls the amount of each component reasonably, makes each component play its own role fully, and makes the obtained composition and eye cream have obvious black removal and whitening effects, increase skin elasticity and improve eye wrinkle phenomenon.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic processing technology, in particular to a whitening and anti-wrinkle composition, a preparation method thereof and an eye cream. BACKGROUND

[0002] Human skin is an organ that is very susceptible to aging. With age, the skin also shows signs of aging: increased wrinkles, skin laxity, yellowing, reduced shine, smoothness and texture, increased pigmentation, and the like. The eye skin is more prominent in terms of fine lines, crow's feet, dark circles and bags.

[0003] In order to delay the aging of the skin around the eyes, in addition to ensuring adequate sleep and developing good habits in daily life, the commonly used method is to use eye cosmetics. The eye cosmetics on the market often not only cannot achieve the purpose of fundamentally protecting the skin, but also can cause skin dependence or strong irritation to the skin, resulting in oil particles and allergic reactions. Therefore, the advent of a healthy and non-irritating eye cream product that can effectively solve the problems of the eye skin has become an urgent need of people. SUMMARY

[0004] In view of the above defects of the prior art, the present application provides a whitening and anti-wrinkle composition, a preparation method thereof and an eye cream to solve the problems raised in the background art.

[0005] To achieve the above purpose, the present application provides the following technical scheme:

[0006] A whitening and anti-wrinkle composition, comprising the following raw materials by weight: 20-30 parts of plant extract, 2-8 parts of petrolatum, 2-5 parts of polyglyceryl-2-diisostearate, 3-8 parts of dipropylene glycol, 1-5 parts of muscle peptide, 2-7 parts of octyldodecanol lauroyl glutamate, 2-4 parts of caffeine, 4-10 parts of vitamin E, 3-9 parts of carbomer, 4-10 parts of butylated hydroxytoluene, 3-15 parts of palmitoyl tripeptide-1, 30-45 parts of water; the plant extract comprises Sapindus mukorossi Gaertn peel extract, tea (Camellia sinensis.) extract, Agave Atrovirens extract, Ziziphus jujuba Mill. extract, Rhizoma Curcumae Longae rhizome extract, Geranium Maculatum extract, Citrus junos seed extract, Cinnamomum cassia Presl bark extract.

[0007] Preferably, the Geranium Punctatum Extract is purchased from Lanzhou Waterless Biological Technology Co., Ltd.; the Agave Americana Extract is purchased from Xi'an Kangnuo Chemical Co., Ltd.

[0008] Preferably, the whitening and anti-wrinkle composition comprises the following raw materials in parts by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of muscleteine, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0009] Preferably, the weight ratio of the plant extract, the Sapindus Diflorus Peel Extract, the Tea Leaf Extract, the Agave Americana Extract, the Ziziphus Jujuba Mill. Extract, the Curcuma Longa Rhizome Extract, the Geranium Punctatum Extract, the Cinnamomum Cassia Bark Extract is (1-3):(0.3-1):(4-6):(1-3):(0.5-2):(3-5):(0.8-1.5):(0.5-1.5).

[0010] Preferably, the preparation method of the plant extract is as follows:

[0011] S1, raw material extraction: the Sapindus Diflorus Peel Extract, the Tea Leaf Extract, the Agave Americana Extract, the Ziziphus Jujuba Mill. Extract, the Curcuma Longa Rhizome Extract, the Geranium Punctatum Extract, the Cinnamomum Cassia Bark Extract are mixed according to the proportion, then 55-75% ethanol by volume fraction is added for reflux extraction for 2-4 times, each time for 25-35 min, the extract is combined, ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed solution;

[0012] S2, 10 5 -10 7 / mL of activated Leuconostoc cremoris culture solution and 10-15% sodium lactate solution by volume fraction are added to the mixed solution, wherein the amount of the Leuconostoc cremoris culture solution added is 3-9% of the mass of the mixed solution, and the amount of the sodium lactate solution added is 0.5-1% of the mass of the mixed solution, and constant temperature aerobic fermentation is carried out at 20-30°C for 10-40h to obtain a plant fermentation product;

[0013] S3, the plant fermentation product is autoclaved to obtain the plant extract.

[0014] Preferably, the product name of the Leuconostoc cremoris freeze-dried powder is Leuconostoc lactis, the number is ATCC19256, and it is purchased from Ningbo Tastwalk Biotech Co., Ltd. The concentration of the Leuconostoc cremoris culture solution is 10 5 -10 7 / mL.

[0015] Preferably, the first activation of the Leuconostoc cryodesiccant powder, the powder is shaken to the bottom, the tip side is sterilized with alcohol, and then knocked open, 0.2-0.3ml sterile water is added to the strain tube, and then gently shaken to mix evenly, and then all the liquid is sucked out with a sterile pipette, and then inoculated into two slopes for culture. The medium composition is: Lacto-casein peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, Tween 80 1g, K2HPO4 2g, Na-acetate 5g, Diamine citrate 2g, MgSO4.7H2O 0.2g, MnSO4.H2O 0.05g, distilled water 1000mL; pH 6.5-6.8. 121℃, 15min.

[0016] Preferably, a preparation method of a whitening and anti-wrinkle composition comprises the following steps:

[0017] S1, at a temperature of 65-70℃, the petrolatum, polyglycerol-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene and water are stirred and mixed until completely dissolved to obtain component A;

[0018] S2, at a temperature of 70-75℃, the plant extract, carnosine, vitamin E and palmitoyl tripeptide-1 are stirred and mixed uniformly to obtain component B;

[0019] S3, under stirring, component B is poured into component A, and continues to be stirred at a stirring speed of 30-45r / min for 5-10min, and then a homogenizer is used to homogenize at a homogenizing speed of 7000-8000r / min and a temperature of 60-70℃ for 8-15min to obtain a mixture;

[0020] S4, the temperature of the mixture obtained in step S3 is reduced to 25-27℃, and then carbomer is added, and the homogenizer is continued to be used at a speed of 7000-8000r / min for 10-20min, and then the temperature is reduced to 25-27℃ to obtain a whitening and anti-wrinkle composition.

[0021] Preferably, a whitening and anti-wrinkle eye cream comprises, by weight percentage, 25-35% of the whitening and anti-wrinkle composition, 4-10% of sodium pyrrolidone carboxylate, 1-5% of glutamic acid, 2-8% of tocopheryl acetate, 0.5-3% of phytosterol, 0.1-0.8% of acetylated sodium hyaluronate, and the balance is water.

[0022] Preferably, the preparation method of the whitening and anti-wrinkle eye cream is

[0023] (1) accurately weigh the various raw materials in the formula, the whitening and anti-wrinkle composition 30%, sodium pyrrolidone carboxylate 8%, glutamic acid 2%, tocopheryl acetate 6%, phytosterol 2%, acetylated sodium hyaluronate 0.5%, and use clean and sterilized containers to hold;

[0024] (2) add the whitening and anti-wrinkle composition, tocopheryl acetate and phytosterol to the oil phase pot at room temperature, stir and heat to 80-85℃, mix evenly, and keep warm for 10-15 minutes;

[0025] (3) add water and sodium pyrrolidone carboxylate to the water phase pot at room temperature, stir and heat to 80-85℃, fully dissolve, and keep warm for 10-15 minutes;

[0026] (4) preheat the emulsification pot to 75-85℃, first draw the water phase pot mixture, then slowly draw the oil phase pot mixture, homogenize and emulsify for 15-30 minutes, and keep warm for 15-25 minutes;

[0027] (5) cool to 35-45℃, add glutamic acid, and fully stir;

[0028] (6) cool to 30-38℃, discharge, and stand for 5-10 minutes;

[0029] (7) after inspection, fill, package, spray code, and store for use.

[0030] Compared with the prior art, the present application has the following beneficial effects:

[0031] (1) the present application selects natural effective components in plants, and substances such as carnosine, vitamin E and palmitoyl tripeptide-1, and controls the amount of each component, so that each component fully plays its role, the obtained composition and eye cream have obvious black removal and whitening effect, increase skin elasticity, improve eye wrinkle phenomenon, and have no skin irritation and do not cause allergic reactions.

[0032] (2) adding Leuconostoc mesenteroides culture solution in the preparation process of plant extract can effectively stimulate natural bioactive compounds contained in the plant extract, including polysaccharides, proteins, vitamins and terpenoids. Leuconostoc mesenteroides is often used to treat intestinal diseases, but there are few reports on the antioxidant effect of polypeptides and exocellular polysaccharides in the product after Leuconostoc mesenteroides and plant extract aerobic fermentation. DETAILED DESCRIPTION

[0033] The technical solutions of the present application will be further described in detail below in combination with specific embodiments.

[0034] Example 1

[0035] A whitening anti-wrinkle composition comprising the following raw materials by weight: 20 parts of plant extract, 2 parts of petrolatum, 2 parts of polyglyceryl-2-diisostearate, 3 parts of dipropylene glycol, 1 part of carnosine, 2 parts of octyldodecyl lauroyl glutamate, 2 parts of caffeine, 4 parts of vitamin E, 3 parts of carbomer, 4 parts of butylated hydroxytoluene, 3 parts of palmitoyl tripeptide-1, and 30 parts of water.

[0036] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange peel extract, and cinnamon bark extract, and the weight ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange peel extract, and cinnamon bark extract is 1:0.3:4:1:0.5:3:0.8:0.5.

[0037] The preparation method of the plant extract is as follows:

[0038] S1, raw material extraction: the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange peel extract, and cinnamon bark extract are mixed in proportion, and then 55% ethanol by volume is added for reflux extraction twice, each for 25 min; the extract is combined, ethanol is recovered, and then cooled at room temperature to obtain a mixed solution;

[0039] S2, 10 5 / mL Leuconostoc mesenteroides culture solution and 10% sodium lactate solution are added to the mixed solution, wherein the amount of the Leuconostoc mesenteroides culture solution added is 3% of the mass of the mixed solution, and the amount of the sodium lactate solution added is 0.5% of the mass of the mixed solution; constant temperature aerobic fermentation is carried out at 20°C for 10 h to obtain a plant fermentation product;

[0040] S3, the plant fermentation product is autoclaved to obtain the plant extract.

[0041] A preparation method of a whitening anti-wrinkle composition, comprising the following steps:

[0042] S1, at a temperature of 65°C, petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene, and water are stirred and mixed until completely dissolved to obtain component A;

[0043] S2, at a temperature of 70°C, the plant extract, carnosine, vitamin E, and palmitoyl tripeptide-1 are stirred and mixed uniformly to obtain component B;

[0044] S3、Under the condition of stirring, pour the B component into the A component, continue to stir at the stirring speed of 30 r / min for 5 min, then use the homogenizer to homogenize at the homogenizing speed of 7000 r / min and the temperature of 60℃ for 8 min, to obtain a mixture;

[0045] S4、Lower the temperature of the mixture obtained in step S3 to 25℃, then add carbomer, continue to use the homogenizer to homogenize at 7000 r / min for 10 min, then lower its temperature to 25℃, to obtain a whitening and wrinkle-removing composition.

[0046] Example 2

[0047] A whitening and wrinkle-removing composition, comprising the following raw materials in parts by weight: 30 parts of plant extract, 8 parts of petrolatum, 5 parts of polyglyceryl-2-diisostearate, 8 parts of dipropylene glycol, 5 parts of muspell, 7 parts of octyldodecyl lauroyl glutamate, 4 parts of caffeine, 10 parts of vitamin E, 9 parts of carbomer, 10 parts of butylated hydroxytoluene, 15 parts of palmitoyl tripeptide-1, and 45 parts of water.

[0048] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, curcuma rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cortex extract, and the weight ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, curcuma rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cortex extract is 3:1:6:3:2:5:1.5:1.5.

[0049] The preparation method of the plant extract is as follows:

[0050] S1, raw material extraction: mix the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, curcuma rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cortex extract according to the proportion, then add 75% ethanol by volume to reflux extract 4 times, each time for 35 min, combine the extract, recover the ethanol, and cool at room temperature to obtain a mixed solution;

[0051] S2, add 10 7 / mL activated Leuconostoc culture solution and 15% sodium lactate solution by volume to the mixed solution, wherein the amount of the Leuconostoc culture solution added is 9% of the mass of the mixed solution, and the amount of the sodium lactate solution added is 1% of the mass of the mixed solution, and perform constant-temperature aerobic fermentation at 30℃ for 40 h to obtain a plant fermentation product;

[0052] S3, sterilize the plant fermentation product by high pressure to obtain a plant extract.

[0053] A preparation method of a whitening and anti-wrinkle composition, comprising the following steps:

[0054] S1, stirring and mixing petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene and water at a temperature of 70℃ until all are dissolved to obtain component A;

[0055] S2, stirring and uniformly mixing plant extract, carnosine, vitamin E and palmitoyl tripeptide-1 at a temperature of 75℃ to obtain component B;

[0056] S3, under the condition of stirring, pouring component B into component A, continuing to stir at a stirring speed of 45r / min for 10min, and then using a homogenizer to homogenize at a homogenizing speed of 8000r / min and a temperature of 70℃ for 15min to obtain a mixture;

[0057] S4, reducing the temperature of the mixture obtained in step S3 to 25℃, adding carbomer, and continuing to homogenize using the homogenizer at a speed of 8000r / min for 20min, and then reducing the temperature to 25℃ to obtain a whitening and anti-wrinkle composition.

[0058] Example 3

[0059] A whitening and anti-wrinkle composition, comprising the following raw materials by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0060] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamon bark extract, and the weight ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamon bark extract is 2:0.7:5:2:1:4:1:1.

[0061] The preparation method of the plant extract is as follows:

[0062] S1, raw material extraction: mixing the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamon bark extract according to the proportion, adding 70% ethanol by volume to reflux extract 3 times, each time for 30min, combining the three extract solutions, recovering the ethanol, and cooling at room temperature to obtain a mixed solution;

[0063] S2, adding the activated bacteria concentration of 10 6 / mL of Leuconostoc culture solution and 12% of sodium lactate solution to the mixed solution, wherein the amount of Leuconostoc culture solution added is 7% of the mass of the mixed solution, and the amount of sodium lactate solution added is 0.8% of the mass of the mixed solution, and carrying out constant temperature aerobic fermentation at 25°C for 30h to obtain a plant fermentation product;

[0064] S3, sterilizing the plant fermentation product by high pressure to obtain a plant extract.

[0065] A preparation method of a whitening and anti-wrinkle composition, comprising the following steps:

[0066] S1, stirring and mixing petrolatum, polyglycerol-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene and water at a temperature of 68°C until all are dissolved to obtain component A;

[0067] S2, stirring and mixing the plant extract, carnosine, vitamin E and palmitoyl tripeptide-1 uniformly at a temperature of 72°C to obtain component B;

[0068] S3, under the condition of stirring, pouring component B into component A, continuing to stir at a stirring speed of 40r / min for 8min, and then using a homogenizer to homogenize at a homogenizing speed of 7500r / min and a temperature of 65°C for 10min to obtain a mixture;

[0069] S4, reducing the temperature of the mixture obtained in step S3 to 25°C, adding carbomer, and continuing to use the homogenizer to homogenize at a speed of 7800r / min for 15min, and then reducing the temperature to 25°C to obtain a whitening and anti-wrinkle composition.

[0070] Example 4

[0071] A preparation method of a whitening and anti-wrinkle eye cream, comprising the following specific steps:

[0072] (1) accurately weighing various raw materials in the formula, whitening and anti-wrinkle composition 30%, pyrrolidone carboxylic acid sodium 8%, glutamic acid 2%, tocopheryl acetate 6%, phytosterol 2%, acetylated sodium hyaluronate 0.5%, and the balance is water.

[0073] using clean and sterilized containers to hold;

[0074] (2) at room temperature, adding the whitening and anti-wrinkle composition, tocopheryl acetate and phytosterol into the oil phase pot, stirring and heating to 82°C, mixing uniformly, and keeping warm for 12 minutes;

[0075] (3) at room temperature, adding water and pyrrolidone carboxylic acid sodium into the water phase pot, stirring and heating to 82°C, dissolving fully, and keeping warm for 12 minutes;

[0076] (4) Preheat the emulsification pot to 80℃, first draw the water phase pot mixture, then slowly draw the oil phase pot mixture, homogenize emulsification for 18 minutes, and keep warm for 20 minutes;

[0077] (5) Cool to 40℃, add glutamic acid, and mix well;

[0078] (6) Cool to 35℃, discharge, and stand for 5-10 min;

[0079] (7) After inspection, fill, package, and spray code, and store for use.

[0080] Comparative Example 1

[0081] The difference between this comparative example and Example 3 is that no lactobacillus culture solution is added in the preparation method of the plant extract, and the rest of the ingredients and steps remain unchanged.

[0082] A whitening and anti-wrinkle composition comprises the following raw materials by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of myo-inositol, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0083] The plant extract comprises Sapindus pernervosus peel extract, tea leaf extract, Agave americana variegata extract, Ziziphus jujuba fruit extract, Curcuma longa rhizome extract, Geranium nepalense extract, Citrus aurantium dulcis seed extract, and Cinnamomum cassia bark extract, and the weight ratio of the Sapindus pernervosus peel extract, tea leaf extract, Agave americana variegata extract, Ziziphus jujuba fruit extract, Curcuma longa rhizome extract, Geranium nepalense extract, Citrus aurantium dulcis seed extract, and Cinnamomum cassia bark extract is 2:0.7:5:2:1:4:1:1.

[0084] The preparation method of the plant extract is as follows:

[0085] S1, raw material extraction: the Sapindus pernervosus peel extract, tea leaf extract, Agave americana variegata extract, Ziziphus jujuba fruit extract, Curcuma longa rhizome extract, Geranium nepalense extract, Citrus aurantium dulcis seed extract, and Cinnamomum cassia bark extract are mixed according to the proportion, 70% ethanol is added for reflux extraction for 3 times, each time for 30 min, the three extraction solutions are combined, the ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed solution;

[0086] S2, a 12% sodium lactate solution by volume is added to the mixed solution, and the amount of the sodium lactate solution added is 0.8% of the mass of the mixed solution, to obtain the plant extract.

[0087] A preparation method of a whitening and anti-wrinkle composition comprises the following steps:

[0088] S1, at temperature 68℃, mix petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauryl glutamate, caffeine, butylated hydroxytoluene and water by stirring until all are dissolved to obtain A component;

[0089] S2, at temperature 72℃, mix plant extract, carnosine, vitamin E, palmitoyl tripeptide-1 by stirring until all are dissolved to obtain B component;

[0090] S3, under stirring, pour B component into A component, continue stirring at stirring speed 40r / min for 8min, then use homogenizer at homogenizing speed 7500r / min and temperature 65℃ to homogenize for 10min to obtain a mixture;

[0091] S4, reduce temperature of the mixture obtained in step S3 to 25℃, then add carbomer, continue using homogenizer at 7800r / min to homogenize for 15min, then reduce its temperature to 25℃ to obtain a whitening and wrinkle-removing composition.

[0092] Comparative Example 2

[0093] The difference between this comparative example and Example 3 is that the formula of plant extract is different, no dark green agave extract is added, and the rest of the components and steps are unchanged.

[0094] A whitening and wrinkle-removing composition, comprising the following raw materials by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauryl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, 40 parts of water.

[0095] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cassia bark extract, and the weight ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cassia bark extract is 2:0.7:0:2:1:4:1:1.

[0096] The preparation method of the plant extract is as follows:

[0097] S1, raw material extraction: mix sapindus pericarp extract, tea leaf extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cassia bark extract according to the ratio, then add 70% ethanol to reflux extract for 3 times, each time for 30min, combine the three extract solutions, recover the ethanol, and cool at room temperature to obtain a mixed solution;

[0098] S2, adding the activated bacteria culture solution of Leuconostoc cremoris and a 12% sodium lactate solution to the mixed solution, wherein the amount of the Leuconostoc cremoris culture solution added is 7% of the mass of the mixed solution, and the amount of the sodium lactate solution added is 0.8% of the mass of the mixed solution, and performing constant temperature aerobic fermentation at 25°C for 30h to obtain a plant fermentation product; 6 / mL of the activated bacteria culture solution of Leuconostoc cremoris and a 12% sodium lactate solution to the mixed solution, wherein the amount of the Leuconostoc cremoris culture solution added is 7% of the mass of the mixed solution, and the amount of the sodium lactate solution added is 0.8% of the mass of the mixed solution, and performing constant temperature aerobic fermentation at 25°C for 30h to obtain a plant fermentation product;

[0099] S3, sterilizing the plant fermentation product by autoclaving to obtain a plant extract.

[0100] A method for preparing a whitening and anti-wrinkle composition, comprising the following steps:

[0101] S1, stirring and mixing petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene, and water at a temperature of 68°C until all are dissolved to obtain component A;

[0102] S2, stirring and mixing the plant extract, carnosine, vitamin E, and palmitoyl tripeptide-1 uniformly at a temperature of 72°C to obtain component B;

[0103] S3, under stirring, pouring component B into component A, continuing to stir at a stirring speed of 40r / min for 8min, and then homogenizing using a homogenizer at a homogenizing speed of 7500r / min and a temperature of 65°C for 10min to obtain a mixture;

[0104] S4, reducing the temperature of the mixture obtained in step S3 to 25°C, adding carbomer, and continuing to homogenize using the homogenizer at a speed of 7800r / min for 15min, and then reducing the temperature to 25°C to obtain a whitening and anti-wrinkle composition.

[0105] Comparative Example 3

[0106] This comparative example differs from Example 3 in that the formulation of the plant extract is different, and the weight fraction of dark green agave extract is increased, and the remaining components and steps are unchanged.

[0107] A whitening and anti-wrinkle composition, comprising the following weight fractions of raw materials: 25 parts of a plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0108] The plant extract comprises Chinese wax tree fruit peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, bergamot seed extract, and cassia bark extract, and the weight ratio of the Chinese wax tree fruit peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, bergamot seed extract, and cassia bark extract is 2:0.7:6:2:1:4:1:1.

[0109] The preparation method of the plant extract is as follows:

[0110] S1, raw material extraction: the Chinese wax tree fruit peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, bergamot seed extract, and cassia bark extract are mixed in proportion, 70% ethanol is added for reflux extraction for 3 times, each time for 30 min, the three extraction solutions are combined, ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed solution;

[0111] S2, 10 6 / mL of Leuconostoc culture solution and 12% sodium lactate solution are added to the mixed solution, the addition amount of the Leuconostoc culture solution is 7% of the mass of the mixed solution, the addition amount of the sodium lactate solution is 0.8% of the mass of the mixed solution, and constant temperature aerobic fermentation is carried out at 25°C for 30 h to obtain a plant fermentation product;

[0112] S3, the plant fermentation product is subjected to high-pressure sterilization to obtain a plant extract.

[0113] A preparation method of a whitening and anti-wrinkle composition, comprising the following steps:

[0114] S1, at a temperature of 68°C, petrolatum, polyglycerol-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene, and water are stirred and mixed until completely dissolved to obtain component A;

[0115] S2, at a temperature of 72°C, the plant extract, carnosine, vitamin E, and palmitoyl tripeptide-1 are stirred and mixed uniformly to obtain component B;

[0116] S3, under stirring, component B is poured into component A, and stirring is continued at a stirring speed of 40 r / min for 8 min, and then a homogenizer is used to homogenize at a homogenizing speed of 7500 r / min and a temperature of 65°C for 10 min to obtain a mixture;

[0117] S4, the temperature of the mixture obtained in step S3 is reduced to 25℃, carbomer is added, and homogenization is continued using the homogenizer at 7800r / min for 15min, then the temperature is reduced to 25℃, to obtain a whitening and wrinkle-fighting composition.

[0118] Comparative Example 4

[0119] The difference between this comparative example and Example 3 is that the formula of the plant extract is different, and no spotted geranium extract is added, and the rest of the ingredients and steps remain unchanged.

[0120] A whitening and wrinkle-fighting composition comprises the following raw materials by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of myo-inositol, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0121] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cassia bark extract, and the weight ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, citrus aurantium seed extract, and cinnamomum cassia bark extract is 2:0.7:5:2:1:0:1:1.

[0122] The preparation method of the plant extract is as follows:

[0123] S1, raw material extraction: the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, citrus aurantium seed extract, and cinnamomum cassia bark extract are mixed according to the proportions, 70% ethanol is added for reflux extraction for 3 times, each time for 30min, the three extraction liquids are combined, the ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed liquid;

[0124] S2, the activated Leuconostoc culture solution with a concentration of 106 / mL and a 12% sodium lactate solution are added to the mixed liquid, the addition amount of the Leuconostoc culture solution is 7% of the mass of the mixed liquid, and the addition amount of the sodium lactate solution is 0.8% of the mass of the mixed liquid, and constant temperature aerobic fermentation is carried out at 25℃ for 30h to obtain a plant fermentation product;

[0125] S3, the plant fermentation product is autoclaved to obtain a plant extract.

[0126] A preparation method of a whitening and wrinkle-fighting composition comprises the following steps:

[0127] S1, at temperature 68℃, mix petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauryl glutamate, caffeine, butylated hydroxytoluene and water by stirring until all are dissolved to obtain component A;

[0128] S2, at temperature 72℃, mix plant extract, carnosine, vitamin E, palmitoyl tripeptide-1 by stirring until all are dissolved to obtain component B;

[0129] S3, under stirring, pour component B into component A, continue stirring at stirring speed 40r / min for 8min, then use homogenizer at homogenizing speed 7500r / min and temperature 65℃ to homogenize for 10min to obtain a mixture;

[0130] S4, reduce the temperature of the mixture obtained in step S3 to 25℃, then add carbomer, continue using homogenizer at 7800r / min for 15min, then reduce its temperature to 25℃ to obtain a whitening and anti-wrinkle composition.

[0131] Comparative Example 5

[0132] The difference between this comparative example and Example 3 is that the formula of plant extract is different, the weight fraction of spot geranium extract is increased, and the rest of the components and steps are unchanged.

[0133] A whitening and anti-wrinkle composition, comprising the following raw materials by weight: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauryl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, 40 parts of water.

[0134] The plant extract comprises sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spot geranium extract, citrus aurantium seed extract, and cinnamon bark extract, and the weight fraction ratio of the sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spot geranium extract, citrus aurantium seed extract, and cinnamon bark extract is 2:0.7:5:2:1:5:1:1.

[0135] The preparation method of the plant extract is as follows:

[0136] S1, raw material extraction: mix sapindus pericarp extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spot geranium extract, citrus aurantium seed extract, and cinnamon bark extract according to the proportion, then add 70% ethanol to reflux extract 3 times, each time for 30min, combine the three extract solutions, recover the ethanol, and cool at room temperature to obtain a mixed solution;

[0137] S2, adding activated strain concentration of 10 6 / mL of Leuconostoc culture solution and 12% sodium lactate solution to the mixed solution, wherein the amount of Leuconostoc culture solution added is 7% of the mass of the mixed solution, and the amount of sodium lactate solution added is 0.8% of the mass of the mixed solution, and carrying out constant temperature aerobic fermentation at 25°C for 30h to obtain a plant fermentation product;

[0138] S3, sterilizing the plant fermentation product by autoclaving to obtain a plant extract.

[0139] A method for preparing a whitening and anti-wrinkle composition, comprising the following steps:

[0140] S1, stirring and mixing petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene and water at a temperature of 68°C until all are dissolved to obtain component A;

[0141] S2, stirring and mixing the plant extract, carnosine, vitamin E and palmitoyl tripeptide-1 uniformly at a temperature of 72°C to obtain component B;

[0142] S3, under stirring, pouring component B into component A, continuing to stir at a stirring speed of 40r / min for 8min, and then using a homogenizer to homogenize at a homogenizing speed of 7500r / min and a temperature of 65°C for 10min to obtain a mixture;

[0143] S4, reducing the temperature of the mixture obtained in step S3 to 25°C, adding carbomer, and continuing to use the homogenizer to homogenize at a speed of 7800r / min for 15min, and then reducing the temperature to 25°C to obtain a whitening and anti-wrinkle composition.

[0144] Comparative Example 6

[0145] This comparative example differs from Example 3 in that the formulation of the plant extract is different, and the weight fractions of dark green agave extract and spotted geranium extract are not added, and the remaining components and steps are unchanged.

[0146] A whitening and anti-wrinkle composition comprising the following weight fractions of raw materials: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

[0147] The plant extract comprises Chinese wax plant peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, bergamot seed extract, and cassia bark extract, and the weight ratio of the Chinese wax plant peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, bergamot seed extract, and cassia bark extract is 2:0.7:0:2:1:0:1:1.

[0148] The preparation method of the plant extract is as follows:

[0149] S1, raw material extraction: the Chinese wax plant peel extract, tea leaf extract, jujube fruit extract, turmeric rhizome extract, bergamot seed extract, and cassia bark extract are mixed in proportion, 70% ethanol is added for reflux extraction for 3 times, each time for 30 min, the three extraction solutions are combined, ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed solution;

[0150] S2, 10 6 / mL of Leuconostoc culture solution and 12% sodium lactate solution are added to the mixed solution, the addition amount of the Leuconostoc culture solution is 7% of the mass of the mixed solution, and the addition amount of the sodium lactate solution is 0.8% of the mass of the mixed solution, and constant temperature aerobic fermentation is carried out at 25°C for 30 h to obtain a plant fermentation product;

[0151] S3, the plant fermentation product is subjected to high-pressure sterilization to obtain a plant extract.

[0152] A preparation method of a whitening and anti-wrinkle composition comprises the following steps:

[0153] S1, at a temperature of 68°C, petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene, and water are stirred and mixed until completely dissolved to obtain component A;

[0154] S2, at a temperature of 72°C, the plant extract, carnosine, vitamin E, and palmitoyl tripeptide-1 are stirred and mixed uniformly to obtain component B;

[0155] S3, under stirring, component B is poured into component A, and stirring is continued at a stirring speed of 40 r / min for 8 min, and then a homogenizer is used to homogenize at a homogenizing speed of 7500 r / min and a temperature of 65°C for 10 min to obtain a mixture;

[0156] S4, the temperature of the mixture obtained in step S3 is reduced to 25°C, carbomer is added, and a homogenizer is used to homogenize at a speed of 7800 r / min for 15 min, and then the temperature is reduced to 25°C to obtain a whitening and anti-wrinkle composition.

[0157] Experimental Example 1: Assay of Intracellular Tyrosinase Activity

[0158] 1.1 Experimental Methods

[0159] L-DOPA is oxidized by tyrosinase to produce brown dopaquinone, a color change indicated by a distinct absorption peak at 475 nm. The level of tyrosinase activity determines the amount of dopaquinone produced, which in turn affects the change in absorbance at 475 nm over a specified time period. The level of tyrosinase activity in cultured cells can be determined by the change in absorbance over the specified time period.

[0160] The concentration during the logarithmic growth phase is 5 × 10⁻⁶. 4 100 μL of B16F10 cells / mL were seeded in 96-well plates, and 1 μM α-MSH was added to induce melanin production. The plates were incubated at 37°C in a CO2 incubator for 24 hours. The supernatant was aspirated, and the cells were washed twice with PBS. 100 μL of DMEM medium containing 10% fetal bovine serum was added to each well, and the plates were incubated in triplicate. The plates were then incubated at 37°C in a CO2 incubator for 24 hours. The supernatant was aspirated, and the cells were washed twice with PBS. 90 μL of 1% Triton X-100 was added to each well for cell lysis. 100 μL of 10 g / L L-DOPA was then added to each well, and the plates were incubated at 37°C in a water bath for 4 hours.

[0161] The experimental group consisted of B16F10 cells + α-MSH + DMEM medium + the compositions prepared in Examples 1-3 + Comparative Examples 1-6;

[0162] The control group consisted of B16F10 cells in α-MSH and DMEM medium.

[0163] The blank control group consisted of B16F10 cells in DMEM medium and 0.2 mol / L phosphate buffer.

[0164] Immediately place under an ELISA reader to measure OD. 475 Values ​​for each well. Average the values ​​from replicates; repeat the experiment three times. Calculate the tyrosinase inhibition rate. Tyrosinase inhibition rate (%) = [1 - (OD0.05)]2 实验组 -OD 空白组 ) / (OD 对照组 -OD 空白组 )]×100%.

[0165] 1.2 Experimental Results

[0166] The tyrosinase inhibition rates are shown in Table 1:

[0167] Table 1 Tyrosinase Inhibition Rate

[0168]

[0169]

[0170] From Table 1, it can be seen that Examples 1-3 and Comparative Examples 1-6 can inhibit the growth of tyrosinase, and Example 3 has the best effect; by comparing Example 3 with Comparative Example 1, it can be seen that the addition of Leuconostoc pseudomesenteroides can effectively stimulate the natural bioactive compounds contained in the plant extracts, and promote the whitening and depigmentation effect of the composition; by comparing with Comparative Example 2, Comparative Example 4 and Comparative Example 6, it can be seen that the Agave fourdericis extract and Geranium maculatum extract affect the final effect of the composition, and only reasonable combination can achieve the depigmentation effect; by comparing with Comparative Example 3 and Comparative Example 5, it can be seen that even if the proportion of the weight fraction of Agave fourdericis extract and Geranium maculatum extract is increased, the tyrosinase inhibition rate is not better than that of Example 3, which shows that only the proportion of the plant extract of Sapindus saponaria fruit peel, tea leaf extract, Agave fourdericis extract, jujube fruit extract, Curcuma longa rhizome extract, Geranium maculatum extract, Citrus aurantium seed extract and Cinnamomum cassia bark extract is appropriate can improve the tyrosinase inhibition rate, and by compounding the plant extract with carnosine, vitamin E, palmitoyl tripeptide-1 and the like, and setting the proportion of the eight components selected from the plant extract to synergize, the composition has better depigmentation effect.

[0171] Experimental Example 2 Evaluation of Anti-wrinkle Effect

[0172] 2.1 Experimental method

[0173] Establishment of culture model: C2C12 cells of mouse myoblasts were inoculated into plates coated with gelatin and cultured in a 37℃, 5% carbon dioxide incubator in a medium containing 2 / 3 MEM and 1 / 3 M199, 2 mmol / L L-glutamine, 100 IU / mL penicillin, 100 μg / mL streptomycin, and 5% fetal bovine serum until a monolayer of myofibrils was formed. Then, spinal cord explants of 10-day-old mouse embryos with dorsal root ganglia were placed on the monolayer of myofibrils, and after co-culturing for 48 h, neurites growing from the explants were observed to contact the muscle cells, and contraction began after 100 h. After co-culturing for 4 weeks, the nerves and muscles had been fully connected to form a model of fully differentiated striated muscle fibers with completely matured neuromuscular junctions (equivalent to motor endplates), and at this stage, the myofibrils could contract regularly, indicating that the culture model had been established.

[0174] Test method: First, observe the contraction frequency of the culture model muscle for 30 seconds using a microscope, observe 5 times, count, and take the average. Next, dilute the compositions of Example 3 and Comparative Examples 1 to 6 of the present application and a commercially available whitening anti-wrinkle solution (main components: vitamin C, fruit acid, squalane, and hyaluronic acid, etc.) as a positive control group with a culture medium at a ratio of 1:100, and then add them to the culture model at a ratio of 5% (v / v), respectively. In addition, set the muscle smeared with sterile water as a blank control group. Observe the muscle contraction frequency for 30 seconds at 1 min, 6 h, and 12 h, count, and record the results. Each test is repeated 5 times.

[0175] 2.2 Experimental results

[0176] The results are shown in Table 2:

[0177] Table 2 Number of times of muscle contraction induced by different products in different time periods

[0178] Sample 1 min 6h 12h Example 3 35 12 3 Comparative Example 1 94 76 47 Comparative Example 2 75 52 33 Comparative Example 3 48 31 12 Comparative Example 4 73 50 25 Comparative Example 5 45 36 16 Comparative Example 6 90 65 31 Blank control group 112 106 98 Positive control group 86 62 41

[0179] As can be seen from Table 2, the number of muscle contractions of the blank control group is high, and the number of muscle contractions is significantly reduced after being treated with the whitening anti-wrinkle composition prepared from Example 3 of the present application, and the number of muscle contractions can be continuously reduced with time, and reduced to 3 times at 12 h, which shows that the whitening anti-wrinkle composition of the present application can achieve the effect of anti-wrinkle and wrinkle removal, and the positive control group can also reduce the number of muscle contractions, but the effect is not as significant as Example 3.

[0180] Example 3

[0181] 3.1 Experimental method

[0182] The culture model is established in the same manner as in Example 2;

[0183] Test method: First, observe the contraction frequency of the culture model muscle for 30 seconds using a microscope, observe 5 times, count, and take the average. Next, dilute the compositions of Example 3 and Comparative Examples 1 to 6 of the present application and a commercially available whitening anti-wrinkle solution (main components: vitamin C, fruit acid, squalane, and hyaluronic acid, etc.) as a positive control group with a culture medium at a ratio of 1:100, and then add them to the culture model at a ratio of 5% (v / v), respectively. In addition, set the muscle smeared with sterile water as a blank control group. Observe the muscle contraction frequency for 30 seconds at 1 min, 6 h, and 12 h, count, and record the results. Each test is repeated 5 times.

[0184] 3.2 Experimental results

[0185] The results are shown in Table 3:

[0186] Table 3 Number of times of muscle contraction induced by Example 4 and a commercially available anti-wrinkle eye cream in different time periods

[0187]

[0188]

[0189] From Table 3, the wrinkle-reducing effect of the eye cream of Example 4 is better than that of the positive control group.

[0190] Experimental Example 4: Test of irritancy

[0191] 4.1 Experimental method

[0192] (1) Red blood cell hemolysis experiment

[0193] Preparation of red blood cell suspension: A healthy rabbit was selected, and 9 mL of blood was taken from the heart, 1 mL of 2% potassium oxalate solution was added, centrifuged, and the supernatant was discarded. The precipitate was diluted to 20 mL with 20 mmol / L PBS solution, and stored at 4°C for standby.

[0194] The compositions provided in Examples 1-3 and Comparative Examples 1-6 were diluted with PBS solution to different concentrations, and five concentration gradients were set for each sample. 10 mL of the diluted sample to be tested was taken, 200 μL of the above red blood cell suspension was added (the final concentrations of the control samples were 5, 10, 20, 50, and 100 mg / mL, respectively), distilled water was used as a complete hemolysis control, and PBS solution was used as a negative control. After mixing gently, incubation was carried out at 37°C for 30 min, centrifugation was carried out at 2000 r / min for 10 min, the supernatant was taken, and the absorbance (A 560 ) at 560 nm was tested by spectrophotometry. The hemolysis rate was calculated according to the following formula:

[0195]

[0196] The sample concentration (HD 50 ) at which 50% of the red blood cells were hemolyzed by the composition was determined according to the regression equation.

[0197] (2) Protein denaturation experiment

[0198] The compositions provided in Examples 1-3 and Comparative Examples 1-6 were diluted with PBS solution to 10 g / L, 10 mL of the diluted sample to be tested was taken, 200 μL of the above red blood cell suspension was added, distilled water was used as a blank control, and 1 mg / mL sodium dodecyl sulfate (SDS) solution was used as a positive control. After mixing gently, incubation was carried out at 37°C for 30 min, centrifugation was carried out at 2000 r / min for 10 min, the supernatant was taken, and the absorbance (A 540 and A 575 at 540 nm and 575 nm, respectively, were tested by spectrophotometry. The protein denaturation index (DI)

[0199]

[0200] R1 = blank control group A 575 blank control group A 540 R2 = experimental group A 575 experimental group A 540 R3 = positive control group A 575 positive control group A 540 .

[0201] The irritancy of the sample to be tested is evaluated according to the L / D value, wherein the L / D value is HD 50 / DI, and the erythrocyte hemolysis test irritancy grading standard is shown in Table 3:

[0202] Table 4 Grading standard

[0203]

[0204]

[0205] 4.2 Experimental results

[0206] The irritancy test results are shown in Table 5:

[0207] Table 5 Irritancy test results

[0208] No. L / D Evaluation Example 1 ﹥100 No irritation Example 2 ﹥100 No irritation Example 3 ﹥100 No irritation Comparative Example 1 ﹥100 No irritation Comparative Example 2 ﹥100 No irritation Comparative Example 3 ﹥100 No irritation Comparative Example 4 ﹥100 No irritation Comparative Example 5 ﹥100 No irritation Comparative Example 6 ﹥100 No irritation

[0209] The results show that the compositions prepared in Examples 1-3 and Comparative Examples 1-6 of the present application are all non-irritating.

[0210] The above description is merely preferred embodiments of the present application, but not to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A whitening anti-wrinkle composition characterized in that, The raw materials include the following weight parts: 20-30 parts of plant extract, 2-8 parts of petrolatum, 2-5 parts of polyglyceryl-2-diisostearate, 3-8 parts of dipropylene glycol, 1-5 parts of carnosine, 2-7 parts of octyldodecyl lauroyl glutamate, 2-4 parts of caffeine, 4-10 parts of vitamin E, 3-9 parts of carbomer, 4-10 parts of butylated hydroxytoluene, 3-15 parts of palmitoyl tripeptide-1, 30-45 parts of water; the plant extract contains soapberry peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange seed extract, and cinnamon bark extract; The weight ratio of soapberry peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange seed extract, and cinnamon bark extract in the plant extract is (1-3):(0.3-1):(4-6):(1-3):(0.5-2):(3-5):(0.8-1.5):(0.5-1.5); The preparation method of the plant extract is as follows: S1, raw material extraction: the soapberry peel extract, tea leaf extract, dark green agave extract, jujube fruit extract, turmeric rhizome extract, spotted geranium extract, orange seed extract, and cinnamon bark extract are mixed in proportion, then 55-75% ethanol by volume is added for reflux extraction for 2-4 times, each time for 25-35 min, the extract is combined, the ethanol is recovered, and then the mixture is cooled at room temperature to obtain a mixed solution; S2, adding Leuconostoc culture solution with a concentration of 10 5 ~ 10 7 CFU / mL and 10-15% sodium lactate solution into the mixed solution, wherein the amount of the Leuconostoc culture solution is 3-9% of the mass of the mixed solution, and the amount of the sodium lactate solution is 0.5-1% of the mass of the mixed solution, and then performing constant temperature aerobic fermentation at 20-30°C for 10-40h to obtain the plant fermentation product; S3, the plant fermentation product is autoclaved to obtain the plant extract.

2. The whitening anti-wrinkle composition according to claim 1, wherein The raw materials include the following weight parts: 25 parts of plant extract, 6 parts of petrolatum, 3 parts of polyglyceryl-2-diisostearate, 6 parts of dipropylene glycol, 3 parts of carnosine, 5 parts of octyldodecyl lauroyl glutamate, 3 parts of caffeine, 8 parts of vitamin E, 5 parts of carbomer, 7 parts of butylated hydroxytoluene, 9 parts of palmitoyl tripeptide-1, and 40 parts of water.

3. The method of claim 1, wherein the whitening anti-wrinkle composition is prepared by mixing the whitening agent and the anti-wrinkle agent in a ratio of 1: 1 to 1:

10. The steps include: ​ S1, at a temperature of 65-70℃, the petrolatum, polyglyceryl-2-diisostearate, dipropylene glycol, octyldodecyl lauroyl glutamate, caffeine, butylated hydroxytoluene, and water are stirred and mixed until completely dissolved to obtain component A; S2, at a temperature of 70-75℃, the plant extract, carnosine, vitamin E, and palmitoyl tripeptide-1 are stirred and mixed uniformly to obtain component B; S3, under stirring, component B is poured into component A, and continues to be stirred at a stirring speed of 30-45 r / min for 5-10 min, then a homogenizer is used to homogenize at a homogenizing speed of 7000-8000 r / min and a temperature of 60-70℃ for 8-15 min to obtain a mixture; S4, the temperature of the mixture obtained in step S3 is reduced to 25-27℃, then carbomer is added, and a homogenizer is used to homogenize at a speed of 7000-8000 r / min for 10-20 min, then the temperature is reduced to 25-27℃ to obtain a whitening and anti-wrinkle composition.

4. A whitening anti-wrinkle eye cream, characterized by, A whitening anti-wrinkle composition comprising, by weight percent, 25-35% of the composition of claim 1, 4-10% of sodium pyrrolidone carboxylate, 1-5% of glutamic acid, 2-8% of tocopheryl acetate, 0.5-3% of phytosterol, 0.1-0.8% of sodium acetylated hyaluronic acid, and the balance being water.

5. The whitening and anti-wrinkle eye cream according to claim 4, characterized in that, A whitening anti-wrinkle composition comprising, by weight percent, 30% of the composition of claim 1, 8% of sodium pyrrolidone carboxylate, 2% of glutamic acid, 6% of tocopheryl acetate, 2% of phytosterol, 0.5% of sodium acetylated hyaluronic acid, and the balance being water.

Images