Full-automatic edible mushroom strain detoxification equipment

Abstract

The application discloses a kind of full-automatic edible mushroom strain detoxification equipment, comprising: inoculation mechanism can realize automatic inoculation transfer between sample mechanism and detoxification mechanism to sample, without multiple artificial transfer strain;Fungus strain detoxification can be completed in a device inside all automation operation;Environment mechanism can regulate and control the environmental conditions in box, including temperature control and air sterilization bacteria virus etc.;Sample mechanism includes: to-be-detoxified test tube and has been detoxified test tube and its conversion mechanical architecture.This scheme completes the function of full-automatic edible mushroom strain detoxification operation of device through the cooperation of several key parts of detoxification, eliminates the disadvantages of manual detoxification operation, realizes full-automatic detoxification, improves the quality and efficiency of edible mushroom strain detoxification;Meanwhile, it solves the problem that opening box and putting in fungus rod and strain during open box inoculation process, which causes repeated disinfection of inoculation box, thereby causing a small amount of residual bacteria, high inoculation contamination rate.

Description

Technical Field

[0001] The invention relates to the technical field of detoxification of edible fungi and other microorganisms, and in particular to a fully automatic edible fungus strain detoxification device. Background Art

[0002] In the process of selecting and cultivating strains for edible fungus production, the strains are easily harmed by viruses, resulting in unstable traits, easy degradation and aging, and it is difficult to maintain good traits for a long time, which seriously restricts the development of edible fungus production.

[0003] There are two main methods for detoxifying edible mushroom strains: one involves culturing them on potato culture plates at temperatures above 32°C; the other involves adding agents such as ribavirin to the potato culture plates. These two methods, combined with the tip-pinching method, achieve a reduction in mycelial virus content through multiple transfers and repeated operations.

[0004] At present, these operations are all performed manually, and the detoxification of edible fungi is very cumbersome and inefficient. It also requires clean benches, incubators and other equipment. Summary of the Invention

[0005] In response to the above troubles in the existing edible fungus strain detoxification operation, the present invention provides a fully automatic edible fungus strain detoxification equipment, which can realize fully automatic microbial detoxification operation steps.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fully automatic edible fungus strain detoxification device, characterized by comprising: a box body and a sample mechanism, an inoculation mechanism, a detoxification mechanism and an environmental mechanism arranged therein;

[0008] The inoculation mechanism is capable of transferring the sample between the sample mechanism and the detoxification mechanism, and the environmental mechanism is capable of adjusting the environmental conditions in the box;

[0009] The sample mechanism includes: a test tube to be detoxified and a test tube that has been detoxified.

[0010] Preferably, the inoculation mechanism comprises: a robotic arm and an inoculation needle;

[0011] The first end of the robotic arm is movably mounted on the crossbeam of the box body, and the inoculation needle is arranged on the second end of the robotic arm.

[0012] Preferably, the inoculation mechanism further comprises: an inoculation needle heating and disinfecting device arranged at the second end of the robotic arm.

[0013] Preferably, the test tube to be detoxified and / or the detoxified test tube is provided with a rubber sealing film.

[0014] Preferably, the sample mechanism further comprises: a turntable for sample tubes to be detoxified; a plurality of the test tubes to be detoxified are arranged circumferentially on the turntable for sample tubes to be detoxified;

[0015] And / or, the sample mechanism further includes: a detoxified sample tube turntable; a plurality of the detoxified test tubes are circumferentially arranged on the detoxified sample tube turntable.

[0016] Preferably, the detoxification mechanism comprises: a wheel-shaped support, a horizontal support, a detoxification tray and an inoculation point;

[0017] The plurality of horizontal supports are rotatably arranged in the box through the wheel-shaped support, the detoxification disk is arranged on the horizontal support, and the inoculation point is arranged on the detoxification disk.

[0018] Preferably, the environmental mechanism includes: an ozone generator and an air conditioner.

[0019] Preferably, the environmental mechanism further comprises: a temperature sensor communicatively connected to the air conditioner.

[0020] Preferably, it further comprises: a monitoring mechanism;

[0021] The monitoring mechanism is capable of monitoring the growth status of the sample at the detoxification mechanism.

[0022] Preferably, the monitoring mechanism includes: a camera and a microcomputer controller;

[0023] The microcomputer controller can control the inoculation mechanism to transfer the sample between the sample mechanism and the detoxification mechanism according to the image information collected by the camera.

[0024] It can be seen from the above technical solutions that the fully automatic edible fungus detoxification equipment provided by the present invention has the following advantages compared with the prior art:

[0025] The automated detoxification device enables automatic detoxification, temperature control, and sterilization. Using a fully automatic detoxification device saves manpower, simplifies operations, improves efficiency, and reduces the risk of bacterial infection.

[0026] 1. The beam-robotic arm structure can automatically perform sampling, detoxification, and inoculation operations, and the inoculation part can be heated, reducing the interference of miscellaneous bacteria in a sterile environment;

[0027] 2. The multi-layer detoxification disc support device can increase the number and efficiency of detoxification in the device;

[0028] 3. The mycelium detoxification status signal collection device can monitor the growth status of the bacteria in the detoxification tray. When detoxification is completed or about to be completed, the robotic arm can be used to inoculate the detoxified mycelium into the culture medium test tube in a timely manner, which is efficient, convenient and highly accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0030] Figure 1 A schematic structural diagram of a fully automatic edible mushroom detoxification device provided in an embodiment of the present invention;

[0031] Figure 2 A schematic structural diagram of a detoxification disk provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0032] First, the technical terms involved in this solution are explained as follows:

[0033] Detoxification: Separate the growth tip tissue of the plant stem tip or fungal hyphae, and culture the separated tissue in a specific culture medium to obtain new seedlings or hyphae that do not contain the original viruses and pathogens.

[0034] The purpose of the present invention is to provide a fully automatic edible fungus strain detoxification device that automates microbial detoxification. The device designed using this technology has a simple structure, high detoxification efficiency, and is easily disseminated on a large scale. This device is a fully automatic system for detoxifying edible fungus strains and is widely applicable to the detoxification and improvement of all edible fungus strains. Transforming the detoxification work of edible fungus strains from being performed by professional technicians to fully automatic equipment not only significantly saves manpower, but also simplifies the operation process, improves work efficiency, and reduces the risk of infection by other bacteria.

[0035] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] The embodiment of the present invention provides a fully automatic edible fungus strain detoxification device, comprising: a box 10 and a sample mechanism, an inoculation mechanism, a detoxification mechanism and an environmental mechanism arranged therein, the structure of which can refer to Figure 1As shown; the inoculation mechanism can transfer the sample between the sample mechanism and the detoxification mechanism, without multiple transfer of strains, and detoxification can be completed in one device; the environmental mechanism can adjust the environmental conditions within the box 10; the sample mechanism includes: a test tube to be detoxified 21 and a detoxified test tube 22.

[0037] As can be seen from the above technical solutions, the fully automatic edible mushroom strain detoxification equipment provided by the embodiments of the present invention achieves the function of automatic detoxification by coordinating the operation of several key detoxification components, thereby improving the various drawbacks of manual detoxification and enhancing the efficiency of mechanized detoxification. It also solves the problem of frequently requiring repeated disinfection of the inoculation box during the inoculation process, which results in a small amount of residual bacteria and a low inoculation success rate due to the need to open the box to place the mushroom sticks and strains.

[0038] In one embodiment of this solution, the inoculation mechanism includes: a robotic arm 31 and an inoculation needle 32; the first end of the robotic arm 31 is movably mounted on the crossbeam 11 of the box 10, and the inoculation needle 32 is disposed at the second end of the robotic arm 31. Figure 1 As shown, the structure of the robotic arm 31 includes a beam moving part, a telescopic part and an inoculation part. The beam moving part can be driven to move left and right on the beam 11, and cooperate with the inoculation part to complete sampling, detoxification and inoculation operations.

[0039] In one embodiment of this solution, the inoculation mechanism further comprises: an inoculation needle heating and disinfecting device 33 provided at the second end of the robotic arm 31. Figure 1 As shown, the inoculation part is provided with an inoculation needle heating and disinfecting device 33, which can be heated in advance before inoculation, detoxification and inoculation to reduce the entry of miscellaneous bacteria.

[0040] In one embodiment of this solution, the test tube to be detoxified 21 and / or the detoxified test tube 22 are provided with a rubber sealing film. The test tube containing the detoxified bacterial strain (i.e., the aforementioned detoxified test tube 21) is provided with a sealing film made of a highly elastic rubber film that automatically seals after the inoculation needle is removed. The test tube containing the culture medium (i.e., the aforementioned detoxified test tube 22) is provided with a sealing film made of a highly elastic rubber film that automatically seals after the inoculation needle is removed.

[0041] In one embodiment of this solution, the sample mechanism further includes: a sample tube turntable 23 to be detoxified; a plurality of test tubes 21 to be detoxified are arranged circumferentially on the sample tube turntable 23; and / or the sample mechanism further includes: a detoxified sample tube turntable 24; a plurality of detoxified test tubes 22 to be detoxified are arranged circumferentially on the detoxified sample tube turntable 24. Figure 1 As shown, the turntable 23 for the test tubes to be detoxified can hold multiple test tubes, and the turntable can rotate to a position that cooperates with the robotic arm 31 for vertical sampling; the turntable 24 for the test tubes of the detoxified samples can rotate to a position that cooperates with the robotic arm 31 for vertical seeding.

[0042] In one embodiment of this solution, the detoxification mechanism 40 includes: a wheel-shaped bracket 41, a horizontal bracket 42, a detoxification disc 43 and an inoculation point 44; a plurality of horizontal brackets 42 are rotatably arranged in the box 10 through the wheel-shaped bracket 41, the detoxification disc 43 is arranged on the horizontal bracket 42, and the inoculation point 44 is arranged on the detoxification disc 43. Figure 1 As shown, the wheel-shaped bracket 41 can be symmetrically provided with multiple horizontal bracket fixing parts, and multiple horizontal brackets 42 are fixed on the wheel-shaped bracket 41. The wheel-shaped bracket 41 is driven by a driving motor and can complete the rotation movement, so that the inoculation part can perform the detoxification operation on the detoxification discs 43 on the multiple horizontal brackets in turn; by setting the wheel-shaped detoxification disc bracket, the number of detoxification strains of the detoxification device can be increased, and the detoxification efficiency of the device can be improved. The detoxification disc 43 is set as a rectangular parallelepiped with an opening at the top, and multiple spaced growth grids are set in the detoxification disc. The growth grids are concave, and the outer peripheral height of the detoxification disc is higher than the spacing height of the internal growth grids, which is convenient for the transfer of the mycelium growth process. The detoxification disc is preferably made of metal. The first growth grid of the detoxification disc is set with PDA culture medium, and the remaining growth grids are ordinary culture medium.

[0043] In one embodiment of this solution, the environmental mechanism 50 includes: an ozone generator 51 and an air conditioner 52. Figure 1 As shown, the ozone generator 51 can be specifically arranged on the upper left side of the box body 10 and can be used for sterilizing the inside of the device. Among them, the air conditioner 52 can be specifically a small dual-temperature air conditioner, and the environmental mechanism 50 can also include a UV generator, which runs for 30 minutes.

[0044] In one embodiment of this solution, the environmental mechanism 50 further includes a temperature sensor that is in communication with the air conditioner 52. Figure 1 As shown, an air conditioner 52 and a temperature sensor are also provided on the upper right side of the box 10. The temperature changes in the box are monitored by the sensors to achieve automatic temperature control effect and provide a suitable temperature environment for the growth of bacteria.

[0045] The fully automatic edible fungus strain detoxification equipment provided by the embodiment of the present invention also includes: a monitoring mechanism 60; the monitoring mechanism 60 is capable of monitoring the growth status of the sample at the detoxification mechanism. In one embodiment of this solution, the monitoring mechanism 60 includes: a camera 61 and a microcomputer controller 62; the microcomputer controller 62 is capable of controlling the inoculation mechanism to transfer the sample between the sampling mechanism and the detoxification mechanism based on the image information collected by the camera 61. Figure 1 As shown, a monitoring mechanism 60 is further provided on the upper right side of the box 10, which identifies the box temperature information and the image information of the mycelium growth status through signal collection and transmission, and prompts temperature control and mycelium inoculation.

[0046] The present invention will be further described below with reference to specific embodiments:

[0047] The present invention provides a fully automatic edible fungus detoxification device, comprising a housing (10). The interior of the housing is divided into three sections. A turntable for sample tubes of edible fungus strains to be detoxified is located at the lower left side of the housing. Detoxified edible fungus strain sample tubes are placed on the turntable. A horizontal beam is located on the left and right sides of the front of the housing. A movable robotic arm is mounted on the beam. The robotic arm inoculates the edible fungus samples to be detoxified onto the detoxification tray culture medium, detoxifies the culture medium on the detoxification tray, and then inoculates the detoxified test tubes from the detoxification tray. A detoxification tray support assembly is located in the middle of the housing. The detoxification tray support assembly consists of a turntable support on both sides and multiple horizontal flat supports connected to the turntable support assembly. The detoxification tray contains a zigzag-shaped detoxification culture medium. A culture medium test tube turntable is located at the lower right side of the housing to receive detoxified strains removed from the detoxification tray by the detoxification robotic arm. A mycelium detoxification status signal collection device, including a camera and a microcomputer controller, is located at the upper right side of the housing. When the detoxification is completed and the mycelium enters the last specific area of ​​the detoxification box, the camera collects the image status of the mycelium growth on the right side of the detoxification disk, transmits the image to the processor, and the microcomputer controller issues an instruction. The robotic arm inoculates the mycelium that has completed the detoxification procedure into the pre-sterilized culture medium tube on the culture medium tube turntable in the lower right corner, thereby completing the automatic detoxification operation of the device.

[0048] Working process:

[0049] 1) Place the test tubes containing the bacteria to be detoxified: Open the door and place the test tubes containing the bacteria to be detoxified on the turntable.

[0050] 2) Internal Environment Disinfection: Turn on the power and start the device. The ozone generator will start to disinfect the entire device for 30 minutes. Simultaneously, the heater above the inoculation needle will heat the robotic arm inoculation needle to 150-250°C for 3-10 minutes. After 3-10 minutes, the inoculation needle will fully cool.

[0051] 3) The robotic arm inoculates to the detoxification tray: The inoculation device of the robotic arm moves to the top of the test tube to be detoxified, the inoculation needle is inserted into the test tube, and the mycelium of the bacteria to be detoxified is taken out. The robotic arm then moves to the top of the detoxification tray bracket, and the inoculation part is aligned with the first growth grid of the detoxification tray (the detoxification tray with an elastic rubber film covering the detoxification culture medium), and the bacteria are inoculated onto the detoxification tray culture medium. The robotic arm then resets and heats to 150-250°C for disinfection for 3-10 minutes; the detoxification tray bracket rotates, and after the next detoxification tray stops at the inoculation position, the robotic arm inoculates the next bacteria to be detoxified and transfers it to the corresponding detoxification tray, completing the second bacteria inoculation operation. Similarly, each bacteria to be detoxified is inoculated into the detoxification tray by the inoculation needle until all inoculations are completed.

[0052] 4) Temperature control inside the box: The temperature inside the box is controlled at 10℃-40℃ to maintain the growth conditions of the fungus and mycelium.

[0053] 5) Detoxification completion: When the mycelium grows to the last growth grid, the camera captures the characteristic image of the mycelium and transmits it to the microcomputer controller, which activates the robotic arm to transfer to the last growth grid. The heater above the inoculation needle heats the robotic arm inoculation needle to 150-250℃ for 3-10 minutes for disinfection. After the inoculation needle has fully cooled after 10 minutes, the mycelium in the inoculation plate is removed and the detoxified bacteria are transferred to the culture medium test tube on the right. The test tube indicator light turns on, completing one round of detoxification. The robotic arm inoculation needle repeats the above steps and can operate on the next strain of bacteria that has been detoxified until all inoculations are completed.

[0054] 6) After multiple rounds of detoxification operations, the strains can be detoxified efficiently and cleanly.

[0055] In summary, the present invention is mainly aimed at a set of fully automatic equipment for detoxification of edible fungi strains, maintaining and restoring the original excellent biological characteristics and production traits of the strains. It is widely used in the detoxification improvement of all edible fungi strains. The detoxification work of edible fungi strains is transformed from being operated by professional technicians to being operated by fully automatic equipment, which not only greatly saves manpower, but also simplifies the operation process, improves work efficiency, and reduces the risk of infection by miscellaneous bacteria. The basic scheme adopted is to design an automated detoxification device, configure relevant functional components to achieve automated detoxification, efficient detoxification operation, including automatic internal sterilization, inoculation, temperature and light culture control, etc. The equipment does not need to transfer strains multiple times, which greatly improves the detoxification efficiency; for those who need to detoxify edible fungi and other fungi, it is easy and efficient to use.

[0056] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0057] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A fully automatic edible fungus strain detoxification device, characterized in that: include: A box (10) and a sample mechanism, an inoculation mechanism, a detoxification mechanism and an environmental mechanism arranged therein; The inoculation mechanism is capable of transferring the sample between the sample mechanism and the detoxification mechanism for inoculation, and the environmental mechanism is capable of adjusting the environmental conditions within the box (10); The sample mechanism comprises: a test tube to be detoxified (21) and a detoxified test tube (22); The sample mechanism further comprises: a sample tube turntable (23) for to-be-detoxified samples; a plurality of the to-be-detoxified test tubes (21) are arranged circumferentially on the sample tube turntable (23); and / or, the sample mechanism further comprises: a detoxified sample tube turntable (24); a plurality of the detoxified test tubes (22) are arranged circumferentially on the detoxified sample tube turntable (24); The detoxification mechanism (40) comprises: a wheel-shaped support (41), a horizontal support (42), a detoxification disk (43) and an inoculation point (44); The plurality of horizontal supports (42) are rotatably arranged in the box (10) via the wheel-shaped support (41), the detoxification disk (43) is arranged on the horizontal support (42), and the inoculation point (44) is arranged on the detoxification disk (43).

2. The fully automatic edible fungus detoxification equipment according to claim 1 is characterized in that: The inoculation mechanism comprises: a robotic arm (31) and an inoculation needle (32); The first end of the robotic arm (31) is movably mounted on the crossbeam (11) of the box (10), and the inoculation needle (32) is arranged on the second end of the robotic arm (31).

3. The fully automatic edible fungus detoxification equipment according to claim 2 is characterized in that: The inoculation mechanism further comprises: an inoculation needle heating and disinfecting device (33) arranged at the second end of the mechanical arm (31).

4. The fully automatic edible fungus detoxification equipment according to claim 1, characterized in that: The test tube to be detoxified (21) and / or the detoxified test tube (22) are provided with an elastic rubber sealing membrane.

5. The fully automatic edible fungus detoxification equipment according to claim 1 is characterized in that: The environmental mechanism (50) includes an ozone generator (51) and an air conditioner (52).

6. The fully automatic edible fungus detoxification equipment according to claim 5, characterized in that: The environmental mechanism (50) further includes a temperature sensor communicatively connected to the air conditioner (52).

7. The fully automatic edible fungus detoxification equipment according to claim 1 is characterized in that: Also includes: Monitoring agencies (60); The monitoring mechanism (60) is capable of monitoring the mycelial growth status of the sample at the detoxification mechanism.

8. The fully automatic edible fungus detoxification equipment according to claim 7, characterized in that: The monitoring mechanism (60) includes: a camera (61) and a microcomputer controller (62); The microcomputer controller (62) can control the inoculation mechanism to transfer the sample between the sample mechanism and the detoxification mechanism based on the image information collected by the camera (61).

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