An anti-wrinkle repair composition containing red camellia extract and its application

Abstract

This invention relates to an anti-wrinkle repair composition containing red camellia extract and its application. The anti-wrinkle repair composition of this invention comprises the following components in weight percentages: polyol 1: 1-25%; red camellia extract: 0.1-15%; creatine: 0.1-2%; carnosine: 0.01-0.5%; recombinant collagen: 0.01-5%; tetrahydrocurcumin inclusions: 1-20%; ergothioneine: 0.0001-1%; water: balance. This invention combats aging through multiple pathways and approaches, providing multi-dimensional skin anti-oxidation and anti-glycation, scavenging free radicals, promoting the production of type I collagen in the skin's extracellular matrix, and strengthening the skin barrier. This composition can be used as an active ingredient in various types of cosmetics, including aqueous solutions, lotions, creams, and shampoos.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics and relates to an anti-wrinkle repair composition containing red camellia extract and its application. Background Technology

[0002] Aging, also known as aging, is an inevitable stage in the biological life process. The skin, located on the body surface, is one of the most prominent organs in the aging process. Skin aging is characterized by dryness, roughness, fine lines, sagging, thickening of the epidermis, and dullness, and is influenced by both internal and external factors. In recent years, many mechanistic theories related to skin aging have been proposed. The latest twelve major markers of aging include: the free radical aging theory, the apoptosis theory, the mitochondrial theory, the immune theory, the gene regulation theory, and the matrix metalloproteinase aging theory. Among these, the free radical theory and the mitochondrial theory are widely accepted and recognized.

[0003] In 1954, scientist Jessman proposed the free radical theory. Free radicals are molecules containing unpaired electrons, characterized by instability and high reactivity. Under normal circumstances, the skin's oxidation and antioxidation systems are in dynamic equilibrium, jointly participating in intercellular and intracellular physiological processes. When the number of free radicals in the body exceeds the body's resistance, the skin experiences oxidative stress. Oxidative stress caused by reactive oxygen species (ROS) can cause three types of harm: 1. Lipid peroxidation, leading to cell membrane damage; 2. Protein damage; 3. Damage to cellular DNA and mitochondria. DNA damage can cause cell cycle arrest and apoptosis, while mitochondrial damage leads to reduced ATP production, causing energy shortages and resulting in skin cell dysfunction, all of which contribute to cellular aging. The mitochondrial theory, proposed by Linnane et al. in 1989, explains the mechanism of skin aging. Currently, scholars are increasingly focusing on the relationship between mitochondria and aging. Mitochondria are important sites for providing energy (ATP) and oxidizing phosphate, and are known as the cell's "powerhouse," playing a crucial role in the production, conversion, and release of ATP. The appearance of dull skin, age spots, wrinkles, and sagging is one of the core mechanisms leading to skin aging.

[0004] Glycation, also known as the Maillard reaction, is a non-enzymatic condensation reaction that occurs at the carbonyl group of a reducing sugar and the free amino group of a protein, producing advanced glycation end products (AGEs). AGEs cross-link with collagen and elastin, reducing collagen solubility and making it difficult to hydrolyze. This reduces collagen activity and metabolism, leading to collagen and elastin loss, resulting in decreased skin elasticity, thinning, reduced moisture content, skin atrophy, and wrinkle formation. Glycation can also cause skin problems such as dullness and roughness.

[0005] According to research reports, the proportion of consumers with sensitive skin is increasing, with thinner stratum corneum and a tendency to experience skin symptoms such as redness and stinging. Therefore, while providing consumers with functional skin care products, it is also necessary and important to enhance the repair of the skin barrier. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings and deficiencies of existing technologies by providing a composition with excellent antioxidant, anti-glycation, and anti-wrinkle effects, and which can enhance the skin barrier, thereby achieving anti-wrinkle repair, through multi-dimensional and multi-pathway design. This composition not only contains extracts of the Chinese specialty plant *Camellia sinensis*, but also active plant components such as tetrahydrocurcumin, creatine, carnosine, and ergothioneine, as well as recombinant collagen, and employs encapsulation technology to help dissolve tetrahydrocurcumin without precipitation.

[0007] Specifically, the mechanism of action of the anti-wrinkle repair composition of the present invention includes the following:

[0008] Red camellia extract can promote the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and has a good antioxidant effect.

[0009] Creatine is a natural nutrient found in the human body that can provide energy for cells;

[0010] Carnosine is a small dipeptide found in the human body, composed of the amino acids β-alanine and L-histidine. Carnosine can exert anti-cellular aging effects by protecting mitochondrial function and reducing the production of reactive oxygen species. It can also chelate metal ions and inhibit cell membrane lipid peroxidation.

[0011] The preparation of recombinant collagen utilizes genetic engineering technology, using the original human collagen gene sequence as a template, and through optimized modification and recombinant expression, yields recombinant human collagen that is highly consistent with human collagen and possesses high biocompatibility and high activity. It has the effect of promoting human collagen synthesis and reducing wrinkles.

[0012] Tetrahydrocurcumin is a natural functional skin-whitening ingredient derived from the hydrogenation of curcumin, isolated from the rhizome of the ginger plant (Curcuma longa). It effectively inhibits the generation of oxygen free radicals and scavenges existing free radicals, exhibiting significant antioxidant activity. It is soluble in organic solvents such as methanol, ethanol, and DMSO, but insoluble in water.

[0013] Ergothioneine is a natural antioxidant that protects cells in the human body and is an important active substance. It can activate the endogenous antioxidant pathway Nrf2 and initiate cellular self-defense.

[0014] The objective of this invention can be achieved through the following methods:

[0015] In a first aspect, the present invention provides an anti-wrinkle repair composition containing red camellia extract, the anti-wrinkle repair composition comprising the following components in weight percentages:

[0016]

[0017]

[0018] As one embodiment of the present invention, the polyol 1 includes at least one of glycerol, butanediol, 1,2-pentanediol, and 1,2-hexanediol.

[0019] Furthermore, the polyol 1 is glycerol or butanediol.

[0020] As one embodiment of the present invention, the red camellia extract comprises water, polyol 2, camellia flower extract, and camellia leaf extract in a mass ratio of 50-80:3-30:1-10:1-10.

[0021] Furthermore, polyol 2 includes at least one of 1,3-propanediol, 1,2-pentanediol, 1,2-hexanediol, and 1,3-butanediol.

[0022] Furthermore, the polyol 2 is 1,3-propanediol.

[0023] As one embodiment of the present invention, the preparation method of the red camellia extract includes: mixing and stirring water, polyol 2, camellia flower extract and camellia leaf extract evenly, filtering to obtain a uniform liquid, which is the red camellia extract.

[0024] As one embodiment of the present invention, the tetrahydrocurcumin inclusion complex comprises water, tetrahydrocurcumin, camellia seed oil, camellia flower extract, camellia leaf extract, polyol 3, PPG-13-decyltetradecyl alcohol polyether-24, and diisopropyl adipate in a mass ratio of 30-60:0.5-3:1-5:0.1-1:0.1-1:15-40:15-30:5-10.

[0025] Furthermore, polyol 3 includes at least one of glycerol, butanediol, phenylpropanol, 1,3-propanediol, and 1,2-pentanediol.

[0026] Furthermore, the polyol 3 is glycerol.

[0027] As one embodiment of the present invention, the preparation method of the tetrahydrocurcumin inclusion complex includes: mixing tetrahydrocurcumin, camellia seed oil, camellia flower extract, camellia leaf extract, diisopropyl adipate, and polyol 3 evenly, adding them to a mixed phase of PPG-13-decyltetradecyl alcohol polyether-24 and glycerol under stirring conditions, and adding water to obtain the tetrahydrocurcumin inclusion complex.

[0028] Secondly, the present invention provides a method for preparing an anti-wrinkle repair composition containing red camellia extract, the preparation method comprising the following steps:

[0029] S1. Add the tetrahydrocurcumin inclusion complex to water and stir to disperse it evenly to form a mixture A;

[0030] S2. Add the remaining components to mixture A in sequence, stir and mix, and filter to obtain the composition.

[0031] In one embodiment of the present invention, in step S2, the mixture is stirred until it is completely uniform.

[0032] Thirdly, the present invention provides the application of an anti-wrinkle repair composition containing red camellia extract in the preparation of cosmetics.

[0033] As one embodiment of the present invention, the dosage form of the cosmetic includes any one of aqueous solution, lotion / cream, and shampoo / conditioner.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] 1. This invention combats aging through multiple pathways and approaches, providing multi-dimensional skin anti-oxidation and anti-glycation, scavenging free radicals, promoting the production of type I collagen in the skin's extracellular matrix, and strengthening the skin barrier. This composition can be used as an active ingredient in various types of cosmetics, including aqueous solutions, lotions, creams, and shampoos.

[0036] 2. The anti-wrinkle repair composition of the present invention contains nano-encapsulations of tetrahydrocurcumin, which helps dissolve tetrahydrocurcumin, prevents crystal precipitation, and is more conducive to the stability and sustained release of active ingredients, and works synergistically with other active ingredients to exert anti-wrinkle and repair effects.

[0037] 3. The anti-wrinkle repair composition of the present invention ensures the stability of tetrahydrocurcumin nano-encapsulations at low temperatures, which is beneficial to the penetration and absorption of active ingredients. This anti-wrinkle repair composition has excellent antioxidant, anti-glycation, collagen synthesis promoting, and cell scratch resistance effects, and has passed safety tests. Attached Figure Description

[0038] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0039] Figure 1 The particle size distribution of the tetrahydrocurcumin inclusions of the present invention is shown, including a particle size distribution graph and a typical particle size distribution table. Detailed Implementation

[0040] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The following examples are implemented under the premise of the technical solution of the present invention, providing detailed implementation methods and specific operating procedures, which will help those skilled in the art to further understand the present invention. It should be noted that the scope of protection of the present invention is not limited to the following embodiments; any adjustments and improvements made under the concept of the present invention are all within the scope of protection of the present invention.

[0041] It should be noted that the preparation or source information of each component in the embodiments or comparative examples of the present invention includes:

[0042] 1. Preparation method and particle size test of tetrahydrocurcumin inclusions: Tetrahydrocurcumin, camellia seed oil, camellia flower extract, tea leaf extract, diisopropyl adipate, and phenylpropanol were mixed evenly, and then added to a mixture of PPG-13-decyltetradecyl alcohol polyether-24 and glycerol under stirring. Finally, water was added to prepare tetrahydrocurcumin inclusions. The mass ratio of water, tetrahydrocurcumin, camellia seed oil, camellia flower extract, tea leaf extract, polyol 3, PPG-13-decyltetradecyl alcohol polyether-24, and diisopropyl adipate was 37:1:3:0.5:0.5:30:20:8; the mass ratio of phenylpropanol to glycerol in polyol 3 was 1:4. The particle size of the obtained inclusions was tested using a Bettersize 3000 particle size analyzer, and the results are shown below. Figure 1 As shown.

[0043] 2. Preparation method of red camellia extract:

[0044] Water, 1,3-propanediol, camellia flower extract, and tea leaf extract were mixed and stirred until homogeneous, and then filtered to obtain a homogeneous liquid; wherein the mass ratio of water, 1,3-propanediol, camellia flower extract, and tea leaf extract was 62.5:27.5:5.0:5.0.

[0045] 3. The recombinant collagen is a recombinant type III collagen aqueous solution raw material purchased from Jiangsu Chuangjian.

[0046] Examples 1-8

[0047] Examples 1-8 provide a method for preparing an anti-wrinkle repair composition, comprising the following steps:

[0048] a. Add the tetrahydrocurcumin inclusion complex to water and stir to disperse it evenly to form a mixture A;

[0049] b. Add the remaining components to mixture A in sequence, stir and mix until completely homogeneous, then filter to obtain the composition.

[0050] The dosage of each component in Examples 1-8 is shown in Table 1:

[0051] Table 1 Components of the Anti-wrinkle Repair Composition

[0052]

[0053] Stability tests were conducted on the anti-wrinkle repair compositions of Examples 1-8. Stability was assessed under six accelerated stability test conditions (room temperature, 40°C, 48°C, 3°C, cycling, and -18°C) for two months. Particular attention was paid to stability at low temperatures, as these conditions affect the transparency of the inclusions in water. This invention ensures low-temperature stability of the compositions even with high inclusion concentrations.

[0054] The stability results of the anti-wrinkle repair compositions in Examples 1-8 are shown in Table 2:

[0055] Table 2

[0056]

[0057]

[0058] Comparative Example 1

[0059] The preparation method of the composition in this comparative example is basically the same as that in Example 3, except that the tetrahydrocurcumin inclusions are replaced with curcumin extract (a polyol solution of tetrahydrocurcumin).

[0060] The tetrahydrocurcumin inclusions were replaced with a solubilizing aqueous solution of another tetrahydrocurcumin. In this comparative example, the polyols selected were methylpropanediol and glycerol, and the surfactant was polysorbate-60, which was used to dissolve the tetrahydrocurcumin. The preparation method is as follows:

[0061] a. Weigh 51.14% methylpropanediol, 11.36% glycerol and 11.36% polysorbate-60 into a container, heat and stir evenly in a 65℃ water bath at a stirring speed of 100 rpm, add 1.14% tetrahydrocurcumin and ensure it is completely dissolved to obtain mixture A.

[0062] b. At 65℃, slowly add the remaining water droplets to mixture A while stirring at 100 rpm until a semi-transparent mixture is formed, thus forming mixture B.

[0063] c. Add the remaining components to the water in sequence, stir and mix evenly, then add mixture B and stir to mix.

[0064] Using this preparation method, a stable composition could not be obtained. After adding mixture B, a bluish tinge and fogging phenomenon occurred. After standing at room temperature for 1-2 days, a precipitate appeared at the bottom, and analysis confirmed that the precipitated solid was tetrahydrocurcumin.

[0065] Comparative Example 2

[0066] The components of this comparative example are basically the same as those in Example 3, except that it does not contain tetrahydrocurcumin inclusions, the content of red camellia extract is 10%, the content of recombinant collagen is 10%, and the content of other components remains unchanged. The preparation method is consistent with that of Example 3, and a clear and transparent liquid is also obtained. The test results are shown in the table below:

[0067] Table 3 Comparison of test results between Comparative Example 2 and Example 3

[0068] serial number DPPH, IC50 (%) AGEs inhibition rate (%) 10% of sample concentration Comparative Example 2 10.68 48.46％ Example 3 0.43 69.21％

[0069] As can be seen from Comparative Example 1, the tetrahydrocurcumin nano-encapsulation used in this invention helps tetrahydrocurcumin to dissolve fully, prevents crystal precipitation, and is more conducive to the stability and sustained release of active ingredients. As can be seen from the comparison between Comparative Example 2 and Example 3, the tetrahydrocurcumin nano-encapsulation can synergistically exert anti-wrinkle and repair effects with other active ingredients, and enhance the antioxidant and anti-glycation effects of the composition.

[0070] Efficacy verification:

[0071] 1. Safety testing of anti-wrinkle repair composition: human patch test

[0072] The anti-wrinkle repair compositions prepared in all the above embodiments were subjected to a human skin occlusive patch test. The test method referred to the human skin patch test in the "Cosmetic Safety Technical Specifications" (2015 edition). The method of the occlusive patch test was as follows: 30 subjects aged 18-60 years were selected, and patches with an area not exceeding 50 mm² were used. 2 A qualified patch test apparatus with a depth of approximately 1 mm was used. 20 μL of the essential oil prepared in the above examples and comparative examples was placed in the small chamber of the patch test apparatus. The negative control well served as a blank control (without any substance). The patch test apparatus was applied to the inner forearm of the subject, and the palm was used to gently press it to ensure even application to the skin for 24 hours. Skin reactions were observed according to the standards in Table 4 at 30 minutes (after the pressure marks disappeared), 24 hours, and 48 hours after removing the patch test apparatus, and the results were recorded. The patch test results are shown in Table 5. The experimental results indicate that no adverse reactions occurred in any of the 30 subjects in Examples 1-8.

[0073] Table 4. Grading Criteria for Skin Reactions in Closed Patch Tests

[0074]

[0075] Table 5 Results of Human Safety Tests

[0076]

[0077] 2. Anti-wrinkle repair composition efficacy test: Antioxidant DPPH test

[0078] All the anti-wrinkle repair compositions prepared in the above examples were subjected to an antioxidant DPPH test. The test method followed the group standard T / SHRH 006-2018 published by the Shanghai Daily Cosmetics Industry Association, "Cosmetics - Free Radical (DPPH) Scavenging Test Method". The test principle is that 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable, long-lived free radical. Its ethanol solution is deep purple and has strong absorption around 520 nm. When a free radical scavenger is present, the light absorption of the DPPH ethanol solution is weakened due to its pairing with a single electron. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, which can be used to evaluate the free radical scavenging ability of the test sample, i.e., the magnitude of its antioxidant activity. The smaller the IC50 value, the stronger the antioxidant capacity. The test results are shown in Table 6.

[0079] Table 6. Antioxidant Test Results

[0080] serial number DPPH, IC50 (%) Example 1 1.91 Example 2 0.71 Example 3 0.43 Example 4 0.84 Example 5 0.50 Example 6 0.52 Example 7 12.79 Example 8 2.03

[0081] 3. Anti-wrinkle repair composition efficacy test: anti-glycation test

[0082] The anti-wrinkle repair compositions prepared in all the above examples were subjected to an anti-glycation AGEs inhibition test. The experimental principle is based on the advanced glycation end products (AGEs) produced by bovine serum albumin (BSA) in the Maillard reaction of glucose. Since some glycosylated proteins exhibit fluorescence, the level of protein glycosylation can be determined by measuring the fluorescence value at an excitation wavelength of 370 nm and an emission wavelength of 440 nm using a fluorescence spectrophotometer. The AGEs inhibition rate of the sample was calculated based on the fluorescence value at the excitation wavelength of 370 nm and the emission wavelength of 440 nm. A higher inhibition rate indicates a better anti-glycation effect. AGEs inhibition rate (%) = [(MG-BC)-(SG-SCG)] / (MG-BC) × 100. The test results are shown in Table 7.

[0083] Table 7 Results of Anti-glycation Test

[0084]

[0085]

[0086] 4. Anti-wrinkle repair composition efficacy test: Test for promoting type I collagen.

[0087] The anti-wrinkle repair compositions prepared in all the above examples were subjected to an anti-glycation AGEs inhibition test. The test method was in accordance with the group standard T / SHRH 031—2020 "Test of Firming and Anti-wrinkle Efficacy of Cosmetics - Determination of Type I Collagen Content in In Vitro Fibroblasts" published by the Shanghai Daily Cosmetics Industry Association. HSF cells in the logarithmic growth phase were seeded into 3 cm diameter cell culture dishes or 6-well cell cultures.

[0088] In the culture plates, after the cells reached 80% confluence, different concentrations of sample were added to the sample groups for further culture. After replacing the culture medium with fresh medium, the cells were cultured for another 24 hours. The cell supernatant was collected, and the content of type I collagen was detected using an ELISA kit. The higher the content, the stronger the anti-wrinkle ability. The test results are shown in Table 8.

[0089] Relative content of type I collagen = T / C × 100%;

[0090] In the formula:

[0091] T—The average content of type I collagen in the test substance;

[0092] C—The average content of type I collagen in the blank / solvent control.

[0093] Table 8 Results of determination of relative content of type I collagen

[0094] serial number concentration Relative value of type I collagen Normal control / 100.00％ Positive control 50ng / ml 134.67％ Example 1 0.25％ 146.58％ Example 2 0.25％ 143.19％ Example 3 0.25％ 170.11％ Example 4 0.25％ 144.94％ Example 5 0.25％ 148.93％ Example 6 0.25％ 137.02％ Example 7 0.25％ 130.61％ Example 8 0.25％ 143.07％

[0095] 5. Anti-wrinkle repair composition repair efficacy test: cell scratch test

[0096] The anti-wrinkle repair compositions prepared in all the above examples were subjected to a cell scratch test for repair. Epidermal keratinocytes are important cells constituting the epidermal layer. When the skin surface is damaged, keratinocytes are stimulated to migrate and repair the damaged area. When cells grow to a monolayer in vitro, a blank area is artificially created on the monolayer. Cells at the edge of the scratch gradually enter the blank area, causing the scratch to heal, which to some extent simulates the in vivo cell migration process. The repair efficacy of the test substance is evaluated by measuring the cell migration rate after sample treatment. Hacat cells in the logarithmic growth phase were washed twice with DPBS, digested with trypsin, and then DMEM medium (10% FBS) was added. After stopping digestion and centrifugation, the supernatant was discarded, and DMEM medium (10% FBS) was added to prepare a cell suspension. The cells were scratched with a 10 μL pipette tip, washed twice with PBS, and then 2 mL of DMEM medium (1% FBS) containing different concentrations of the sample was added to each well. The sample concentration was 1%. Twenty-four hours later, photographs were taken of the same field of view of the sample group, with a blank control in DMEM medium (1% FBS). The scratch distance (d) at three locations at each time point was measured, and cell migration rate was calculated. The in vitro scratch assay was used to assess cell migration ability, and the ability of the sample to promote cell migration was evaluated based on the degree of wound healing. The test results are shown in Table 9.

[0097] Cell migration rate (%) = (d0h - d24h) / d0h * 100%

[0098] All experimental data are expressed as mean ± SEM data.

[0099] Table 9 Results of Cell Scratch Test

[0100] serial number Cell migration rate (%) Blank control group 29.24 Example 1 51.24 Example 2 59.63 Example 3 70.43 Example 4 73.08 Example 5 59.29 Example 6 52.33 Example 7 41.01 Example 8 40.48

[0101] The main innovations of this invention are as follows: 1. Tetrahydrocurcumin is the main active ingredient in turmeric root extract, possessing excellent chemical stability and appearing as a white powder, overcoming the defects of ordinary turmeric extracts, which are chemically unstable and easily stain the skin. However, tetrahydrocurcumin is insoluble in water and oil, making it difficult to dissolve and prone to precipitation during formulation application, thus failing to achieve the desired efficacy. This invention utilizes nano-encapsulation technology to dissolve and encapsulate tetrahydrocurcumin, not only enhancing its solubility but also resulting in smaller particle sizes that facilitate penetration and application; 2. Based on the mechanism of skin aging and combined with mainstream market trends, this invention seeks corresponding active ingredients for synergistic effects, acting on the skin through four levels and six pathways. Its scientific and rational design provides a gentle, safe, and effective way to combat skin aging.

[0102] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. An anti-wrinkle repair composition containing camellia extract, characterized in that, The anti-wrinkle repair composition comprises the following components in weight percentages: Polyol 1 1-25%; Red camellia extract 0.1-15%; Creatine 0.1-2%; Carnosine 0.01-0.5%; Recombinant collagen 0.01-5%; Tetrahydrocurcumin inclusions 1-20%; Ergothioneine 0.0001-1%; Water balance; The polyol 1 includes at least one of glycerol, butanediol, 1,2-pentanediol, and 1,2-hexanediol; The tetrahydrocurcumin inclusion complex comprises water, tetrahydrocurcumin, camellia seed oil, camellia flower extract, camellia leaf extract, polyol 3, PPG-13-decyltetradecyl alcohol polyether-24, and diisopropyl adipate in a mass ratio of 30-60:0.5-3:1-5:0.1-1:0.1-1:15-40:15-30:5-10; The polyol 3 includes at least one of glycerol, butanediol, phenylpropanol, 1,3-propanediol, and 1,2-pentanediol.

2. The anti-wrinkle repair composition according to claim 1, characterized in that, The preparation method of the tetrahydrocurcumin inclusion complex includes: mixing tetrahydrocurcumin, camellia seed oil, camellia flower extract, camellia leaf extract, diisopropyl adipate, and polyol 3 evenly, adding them to a mixed phase of PPG-13-decyltetradecyl alcohol polyether-24 and glycerol under stirring, and adding water to obtain the tetrahydrocurcumin inclusion complex.

3. A method for preparing an anti-wrinkle repair composition containing camellia extract as described in any one of claims 1-2, characterized in that, The preparation method includes the following steps: S1. Add the tetrahydrocurcumin inclusion complex to water and stir to disperse it evenly to form a mixture A; S2. Add the remaining components to mixture A in sequence, stir and mix, and filter to obtain the composition.

4. The use of an anti-wrinkle repair composition containing red camellia extract as described in any one of claims 1-2 in the preparation of cosmetics.

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