A drug for controlling the growth of Fusarium spp. and banana diseases and its use

Abstract

The present invention provides an effective and safe green fungicide and its application. The green fungicide is a composition containing nicotinamide adenine dinucleotide (NAD). Nicotinamide adenine dinucleotide (NAD) treatment significantly inhibits the growth of Fusarium solani on potato dextrose agar (PDA); at the same time, after NAD treatment of bananas, the resistance of banana fruits to Fusarium solani is improved, resulting in a significant decrease in the pathogenicity of Fusarium solani to banana fruits, and the diameter of the lesions on the banana peel after NAD treatment is significantly smaller than that of the control group. Therefore, NAD treatment can be used to prepare a new antifungal preservative and is applied in the field of prevention and control of post-harvest fungal diseases of fruits.

Description

Technical Field

[0001] The invention belongs to the field of green ecological prevention and treatment of plant diseases, and specifically relates to a novel antibacterial agent and application of the antibacterial agent in controlling Fusarium thaliana diseases. Background Art

[0002] Crown rot is one of the important fungal diseases of bananas after harvest. The increasing severity and spread of crown rot has led to a significant decrease in the nutrition and quality of bananas after harvest, causing major economic losses. Due to the spread and improper prevention and control of banana crown rot, it has brought great difficulties to the storage and transportation of bananas after harvest in my country, and has gradually become one of the main diseases of bananas after harvest. Fusarium proliferatum is one of the main pathogens causing crown rot of bananas after harvest. At present, chemical agents must be used for prevention and control, which has a certain impact on the fruit and is not conducive to the health of the eater. Therefore, screening green fungicides that are harmless to the human body, effective and safe to control the growth and infection of Fusarium proliferatum is of great significance to alleviate the further spread of banana crown rot after harvest, maintain the quality of bananas and extend the storage period.

[0003] Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor and redox carrier involved in a variety of cellular processes and is non-toxic to humans. Studies have shown that NAD may be involved in the interaction between pathogens and plants (Pangenomic analysis reveals plant NAD+ manipulation as an important virulence activity of bacterial pathogen effectors). However, there are currently no reports on the role and application of NAD in controlling postharvest fruit diseases. Summary of the invention

[0004] Based on the problems existing in the background technology, the purpose of the present invention is to provide an effective and safe green fungicide and its application.

[0005] In one aspect, the present invention provides an antibacterial agent, characterized in that the antibacterial agent comprises nicotinamide adenine dinucleotide (NAD) and a buffer.

[0006] In a preferred embodiment, the buffer is a neutral or weakly acidic buffer.

[0007] Furthermore, the neutral or weakly acidic buffer solution may be pure water.

[0008] In another preferred embodiment, the antibacterial agent of the present invention further contains a surfactant for enhancing the adhesion of the liquid on the plant surface.

[0009] In another preferred embodiment, the antibacterial agent of the present invention may further contain a thickener, a stabilizer, a solubilizing agent, and the like.

[0010] Furthermore, the antibacterial agent contains nicotinamide adenine dinucleotide (NAD) at a concentration of 3 to 20 mM.

[0011] In a preferred embodiment, the antibacterial agent contains nicotinamide adenine dinucleotide (NAD) at a concentration of 4 to 16 mM.

[0012] Furthermore, the antibacterial agent is a fruit preservative.

[0013] Furthermore, the antibacterial agent has the effect of inhibiting the growth of Fusarium proliferatum.

[0014] In another aspect, the present invention provides the use of nicotinamide adenine dinucleotide (NAD) in the preparation of an antibacterial agent.

[0015] In a preferred embodiment, the antibacterial agent of the present invention contains nicotinamide adenine dinucleotide (NAD) and a buffer.

[0016] Furthermore, the buffer is a neutral or weakly acidic buffer.

[0017] Furthermore, the buffer solution is pure water.

[0018] Furthermore, the antibacterial agent contains nicotinamide adenine dinucleotide (NAD) at a concentration of 3 to 20 mM, preferably 4 to 16 mM.

[0019] Furthermore, the antibacterial agent can achieve an antibacterial effect by soaking, spraying or applying.

[0020] Furthermore, the object to be soaked, sprayed or applied may be fruit or packaging material for packaging fruit.

[0021] In another aspect, the present invention provides use of the above-mentioned antibacterial agent in inhibiting the growth of pathogenic bacteria.

[0022] In another aspect, the present invention provides use of the above-mentioned antibacterial agent in fruit preservation.

[0023] In a preferred embodiment, the fruit preservation is achieved by inhibiting pathogenic bacteria with an antibacterial agent containing nicotinamide adenine dinucleotide (NAD).

[0024] Furthermore, the antibacterial agent contains nicotinamide adenine dinucleotide (NAD) at a concentration of 3 to 20 mM, preferably 4 to 16 mM.

[0025] Furthermore, the buffer is a neutral or weakly acidic buffer.

[0026] Furthermore, the buffer solution is pure water.

[0027] Furthermore, the antibacterial agent contains nicotinamide adenine dinucleotide (NAD) at a concentration of 4 to 16 mM.

[0028] Furthermore, the antibacterial agent can achieve an antibacterial effect by soaking, spraying or applying.

[0029] Furthermore, the object to be soaked, sprayed or applied may be fruit or packaging material for packaging fruit.

[0030] In a preferred embodiment, the aforementioned antibacterial agent can inhibit the growth of Fusarium solani.

[0031] Compared with the prior art, the present invention has the following advantages:

[0032] 1) The present invention provides a green fungicide which is harmless to human body and effective and safe.

[0033] 2) The present invention provides a new use of NAD, namely, for preparing a green fungicide that is harmless to the human body and is effective and safe.

[0034] 3) The present invention provides for the first time the application of NAD in controlling post-harvest diseases of fruits. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The beneficial effects of the present invention are described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0036] Figure 1 Effect of NAD treatment on the growth of Fusarium solani on PDA plates. (A) Phenotypes of Fusarium solani treated with different concentrations; (B) Statistical histogram of colony diameters treated with the minimum inhibitory concentration of 4 mM, p<0.05, n=3.

[0037] Figure 2 Effects of NAD treatment on banana infection by Fusarium solani. (A), banana phenotype; (B), histogram of plaque diameter, p<0.05, n=16. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0040] Example 1: Antibacterial effect of NAD on culture medium

[0041] Fusarium spp. was cultured on PDA plates at 28°C for 7 days. The spores were washed with sterile water and filtered through gauze. The filtrate was collected and the spore concentration was adjusted to 1 × 10 6 spores / ml. 10 μl of the above spores were dropped into the PDA medium containing 0 mM NAD and 0 mM, 1 mM, 2 mM, 4 mM, 8 mM, and 16 mM NAD, respectively. After 7 days of culture, the effect of NAD on the growth of Fusarium was compared. Figure 1 A, Starting from 4 mM, NAD treatment can inhibit the growth of Fusarium solani;

[0042] We further selected the minimum inhibitory concentration of 4mM and conducted three repeated tests. The colony diameters of the treated group were statistically smaller than those of the control group (0mM CK( Figure 1 B). This indicates that NAD can effectively control the growth of Fusarium solani.

[0043] Example 2: Antibacterial effect of NAD soaked fruit

[0044] Banana (Musa acuminata AAAgroup, cv. Cavendish) fruits were selected and harvested on a sunny day, 80% ripe, free of mechanical damage and pests and diseases. The banana fruits were randomly divided into two groups. The control group and the experimental group were immersed in clean water and 4 mM NAD aqueous solution for 5 min, respectively. The banana fruits after immersion were dried. The acupuncture method was used to inoculate Fusarium spores: 10 μl of Fusarium spores (1×10 6 spores / ml) were dropped onto the punctured part of the banana fruit. All banana fruits were stored in an incubator at 25°C and 85% relative humidity to observe the disease. Seven days after inoculation, it was first observed with the naked eye that the banana peel treated with NAD had less disease ( Figure 2 A), and the diameter of the plaque on the banana peel after NAD treatment was significantly smaller than that of the control group CK ( Figure 2 B). This indicates that NAD treatment can effectively control the occurrence of crown rot.

[0045] Example 3: Antibacterial agent containing NAD

[0046] 1. Antibacterial agents for soaking or spraying

[0047] Components Content (concentration) NAD 4~16mM Cosolvent (optional): glycerol, ethylene glycol, etc. 10% to 20% (ethylene glycol) or 5% to 10% (glycerol) water

[0048] 2. Antibacterial agent for spraying in forests

[0049]

[0050] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to the above embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Nicotinamide adenine dinucleotide in the preparation of inhibitory Fusarium spp. Fusarium proliferatum ) growth inhibitor, characterized in that, The active ingredient of the antibacterial agent is nicotinamide adenine dinucleotide.

2. The use according to claim 1, characterized in that The antibacterial agent contains nicotinamide adenine dinucleotide and a buffer.

3. An antibacterial agent containing nicotinamide adenine dinucleotide is effective in inhibiting the growth of Fusarium spp. Fusarium proliferatum ) in growth, characterized in that, The active ingredient of the antibacterial agent is nicotinamide adenine dinucleotide.

4. An application of an antibacterial agent containing nicotinamide adenine dinucleotide in fruit preservation, characterized in that: The active ingredient of the antibacterial agent is nicotinamide adenine dinucleotide, and the fruit preservation is achieved by inhibiting the growth of Fusarium erythrorhizium ( Fusarium proliferatum )’s growth is achieved.

5. The use according to claim 4, characterized in that The antibacterial agent contains nicotinamide adenine dinucleotide at a concentration of 4 to 16 mM.

6. The use according to claim 4, characterized in that The antibacterial agent can achieve the antibacterial effect by soaking, spraying or applying.

7. The use according to claim 6, characterized in that The object of soaking, spraying or applying is fruit or packaging material for packaging fruit.

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