Multi-site laser acupuncture diagnosis and treatment integrated system and method

Abstract

The application relates to the technical field of phototherapy, and discloses a multi-site laser acupuncture diagnosis and treatment integrated system and method, which comprises the following: a probe comprising an acupuncture needle and an imaging optical fiber, the imaging optical fiber being arranged in the interior of the acupuncture needle; an optical imaging diagnosis device comprising an excitation light path module, an optical fiber pre-calibration module and a signal detection module; and a control terminal connected with the excitation light path module, the optical fiber pre-calibration module and the signal detection module respectively. The embodiment of the application overcomes the defect that the existing invasive laser acupuncture technology cannot realize real-time imaging of cells around an acupoint or a local area of other sites in an acupuncture stimulation process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of phototherapy, in particular to a multi-site laser acupuncture diagnosis and treatment integrated system and method. BACKGROUND

[0002] With the development of laser medical technology, photobiomodulation has become a common treatment method in clinical practice. Photobiomodulation can induce the body to produce benign biological stimulation and photochemical reaction, so as to promote wound healing by promoting tissue repair, reducing inflammation and producing analgesic effect, and will not cause irreversible tissue damage. Laser acupuncture takes advantage of the photobiomodulation technology, and directly acts on the acupoints and other parts in the form of "light needle" through the light energy of low-power laser beam, so as to achieve the light stimulation similar to acupuncture or related therapy on the acupoints and other parts. Among them, for deep acupoint treatment and intravascular irradiation therapy, laser acupuncture uses optical fiber and acupuncture needle to directly introduce laser into the internal tissue for treatment. However, the output end of the optical fiber will generate speckle due to its own scattering medium characteristics. The existence of speckle makes it difficult to perform real-time clear and effective functional imaging on the local area of the acupuncture tip during laser acupuncture. At present, there is no system integrating imaging diagnosis and focused treatment functions. SUMMARY

[0003] Therefore, the present application provides a multi-site laser acupuncture diagnosis and treatment integrated system and method to solve the technical problem that the acupuncture needle cannot realize imaging diagnosis.

[0004] In a first aspect, the present application provides a multi-site laser acupuncture diagnosis and treatment integrated system, comprising: a probe comprising a plurality of acupuncture needles and an imaging optical fiber, the distal end of the imaging optical fiber comprising at least one multi-mode optical fiber, each of the multi-mode optical fibers being arranged inside a corresponding acupuncture needle; an optical imaging diagnosis device comprising an excitation light path module, an optical fiber pre-calibration module, and a signal detection module, the excitation light path module being configured to generate an excitation light beam and a reference light beam and focus the excitation light beam into the multi-mode optical fiber, the optical fiber pre-calibration module being configured to collect a hologram generated by interference between the reference light beam and the excitation light beam; the signal detection module being configured to collect a fluorescence signal generated by a to-be-measured object scanned by the probe; and a control terminal connected to the excitation light path module, the optical fiber pre-calibration module, and the signal detection module, respectively, and configured to control the optical fiber pre-calibration module to collect, at equal intervals, holograms generated by interference between the reference light beam and the excitation light beam, calculate an output light field of the imaging optical fiber based on the holograms, calculate a transmission matrix based on the output light field, calculate an input light field based on the transmission matrix and a target output light field, control parameters of the excitation light beam generated by the excitation light path module based on the input light field, generate a diagnosis image based on the fluorescence signal, and control laser generated by the excitation light path module based on preset parameters and make the generated laser irradiate a to-be-treated part through the acupuncture needles.

[0005] Optionally, the excitation light path module comprises: a laser configured to output a laser beam; a first 4f system configured to receive the laser beam generated by the laser and expand and collimate the laser beam; a power adjustment module configured to adjust the output power of the laser beam and convert the laser beam into an excitation light beam and a reference light beam for output; a second 4f system configured to expand and collimate the excitation light beam; an optical modulation module configured to modulate the excitation light beam expanded and collimated by the second 4f system, so that the excitation light beam forms a focused spot at the exit end of the imaging optical fiber to scan the to-be-measured object; a third 4f system internally provided with a diaphragm configured to filter out redundant diffraction order reflected light of the excitation light beam modulated by the optical modulation module, except for target field information; and a coupling module configured to couple the excitation light beam exiting the third 4f system into the imaging optical fiber and separate the excitation light beam and the fluorescence signal.

[0006] Optionally, the power adjustment module comprises: a half-wave plate configured to adjust the polarization direction of the laser beam expanded and collimated by the first 4f system; and a first polarization beam splitter prism configured to cooperate with the half-wave plate to adjust the output power of the laser beam and convert the laser beam into an excitation light beam and a reference light beam for output.

[0007] Optionally, the coupling module comprises: a dichroic mirror for separating the excitation light beam and the fluorescent signal; and a first objective lens for coupling the excitation light beam reflected by the third 4f system and the dichroic mirror into the imaging optical fiber.

[0008] Optionally, the optical fiber pre-calibration module comprises: a microscopic imaging system for receiving the excitation light beam emitted by the imaging optical fiber and performing end face imaging on the imaging optical fiber according to the excitation light beam; a beam splitting prism for receiving the reference light beam and the excitation light beam passing through the microscopic imaging system and causing interference between the reference light beam and the excitation light beam to generate a hologram; a reflection unit for reflecting the reference light beam to the beam splitting prism; a spatial pinhole filtering system for filtering stray light on the way of the reflection unit reflecting the reference light beam to the beam splitting prism; and a surface detector for collecting the hologram and sending the hologram to the control terminal.

[0009] Optionally, the signal detection module comprises: a second lens for focusing the fluorescent signal generated by the object to be detected to a point detector; and the point detector for collecting the fluorescent signal and sending the fluorescent signal to the control terminal.

[0010] Optionally, the light modulation module is a digital micromirror array or a liquid crystal phase spatial light modulator.

[0011] Optionally, the system further comprises a sample control module for carrying and moving a sample.

[0012] In a second aspect, the present application provides a multi-site laser acupuncture diagnosis and treatment integrated method, applied to the multi-site laser acupuncture diagnosis and treatment integrated system of the first aspect, the method comprising: determining a multi-site laser acupuncture diagnosis and treatment mode, wherein the multi-site laser acupuncture diagnosis and treatment mode comprises a fiber pre-calibration mode, an imaging diagnosis mode and a focused treatment mode; removing the sample control module when the multi-site laser acupuncture diagnosis and treatment mode is the fiber pre-calibration mode; calculating the transmission matrix of each multi-mode optical fiber in the probe respectively, wherein the calculation of the transmission matrix of one multi-mode optical fiber comprises: locating the end face of the multi-mode optical fiber at the working distance of the second objective lens in the fiber pre-calibration module; pre-loading a pre-hologram in the light modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; controlling the proportion of the excitation light beam and the reference light beam generated by the excitation light path module, and phase-modulating the excitation light beam, and controlling the fiber pre-calibration module to collect the hologram generated by the interference of the reference light beam and the excitation light beam at equal intervals, and calculating the output light field of the imaging optical fiber according to the hologram, so as to calculate the transmission matrix according to the output light field; when the multi-site laser acupuncture diagnosis and treatment mode is the imaging diagnosis mode, a plurality of sites are imaged by a plurality of acupuncture needles and imaging optical fibers in time-sharing manner, wherein the imaging process of one site comprises: loading a pre-hologram in the light modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; calculating the input light field according to the transmission matrix of the corresponding multi-mode optical fiber and the target output light field, and controlling the parameters of the excitation light beam generated by the excitation light path module according to the input light field to make the exit end of the multi-mode optical fiber form a focused light spot to scan the object to be measured, collecting the fluorescence signal generated by the object to be measured scanned by the probe through the signal detection module, and generating a diagnosis image according to the fluorescence signal, so as to realize imaging of the local area of the site of one multi-mode optical fiber; when the multi-site laser acupuncture diagnosis and treatment mode is the focused treatment mode, the laser generated by the excitation light path module is controlled according to the preset parameters, and the generated laser is irradiated on the treatment site through the probe.

[0013] From the above technical solutions, it can be seen that the embodiments of the present application have the following advantages:

[0014] The multi-site laser acupuncture diagnosis and treatment integrated system and method provided by the present application calculate the transmission matrix of the imaging optical fiber through the fiber pre-calibration module, calculate the input light field according to the transmission matrix and the target output light field, control the parameters of the excitation light beam generated by the excitation light path module according to the input light field to make the exit end of the imaging optical fiber form a focused light spot to scan the object to be measured, generate a diagnosis image according to the fluorescence signal generated by the object to be measured, realize high-resolution optical focusing of the local area of the acupoint site or other site, and realize rapid scanning of the focal point in the field of view to obtain a real-time clear and effective image, overcoming the defect that the existing invasive laser acupuncture technology has not been able to realize real-time imaging of the cells around the acupoint or the local area of other sites during the acupuncture stimulation process. Attached Figure Description

[0015] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the probe structure according to an embodiment of the present invention;

[0017] Figure 2 This is a block diagram of the multi-site laser acupuncture diagnosis and treatment integrated system according to an embodiment of the present invention;

[0018] Figure 3 This is a schematic diagram of the excitation optical path module according to an embodiment of the present invention;

[0019] Figure 4 This is a schematic diagram of the multi-site laser acupuncture diagnosis and treatment integrated system according to an embodiment of the present invention;

[0020] Figure 5 This is a flowchart of the multi-site laser acupuncture diagnosis and treatment integrated system in this embodiment of the invention;

[0021] Figure 6 This is a diagram illustrating the effect of focusing the light spot after passing through a multimode optical fiber, according to an embodiment of the present invention.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1-Laser; 2-First 4f system; 3-Half-wave plate; 4-First polarizing beam splitter; 5-Second 4f system; 6-First reflecting mirror; 7-Second reflecting mirror; 8-Optical modulation module; 9-Third 4f system; 10-Dichroic mirror; 11-First objective lens; 12-Imaging fiber; 13-Second objective lens; 14-Tube mirror; 15-Third reflecting mirror; 16-Fourth reflecting mirror; 17-Beam splitter; 18-Third objective lens; 19-Pinhole; 20-First lens; 21-Surface detector; 22-Second lens; 23-Point detector; 24-Sample carrier; 25-Control terminal; 0201-Third lens; 0202-Fourth lens; 0501-Fifth lens; 0502-Sixth lens; 0901-Seventh lens; 0902-Aperture stop; 0903-Ninth lens. Detailed Implementation

[0024] In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0025] In view of the technical problem that the acupuncture needle cannot realize diagnosis and treatment integration at present, the embodiment of the present application provides a multi-site laser acupuncture diagnosis and treatment integration system. Figure 1 、 Figure 2 、 Figure 3 and Figure 4 The system comprises:

[0026] The probe comprises a plurality of acupuncture needles and an imaging optical fiber 12, and the distal end of the imaging optical fiber 12 comprises at least one multi-mode optical fiber, and each multi-mode optical fiber is arranged in the interior of a corresponding acupuncture needle.

[0027] Exemplarily, the imaging optical fiber 12 is 3 multi-mode optical fibers, which are only examples and can also be 4, 5, etc. They are arranged in a square shape at the distal end of the optical fiber bundle, and are divided into 3 branches at the proximal end, and each branch has a single optical fiber. The acupuncture needle is hollow, and each branch of the proximal end of the imaging optical fiber 12, i.e. each multi-mode optical fiber, is inserted into the interior of the 3 hollow acupuncture needles, and the proximal end of each multi-mode optical fiber is adhered to the tip of the acupuncture needle.

[0028] The optical imaging diagnosis device comprises an excitation light path module, an optical fiber pre-calibration module and a signal detection module, the excitation light path module is used for generating an excitation light beam and a reference light beam and focusing the excitation light beam to the multi-mode optical fiber, the optical fiber pre-calibration module is used for collecting a hologram generated by the interference of the reference light beam and the excitation light beam; and the signal detection module is used for collecting a fluorescence signal generated by a to-be-measured object scanned by the probe.

[0029] The main function of the excitation light path module is to generate laser and focus the laser to the probe. After pre-calibration, the effect diagram of the spot focusing of the probe is as shown in Figure 6 , scanning excitation is realized, and in the embodiment of the present application, the excitation light path module divides the generated laser into an excitation light beam and a reference light beam. The optical fiber pre-calibration module is used for collecting a hologram generated by the interference of the reference light beam and the excitation light beam, and the transmission matrix of the imaging optical fiber 12 is calculated by collecting the hologram. The signal detection module is connected with the probe and is used for receiving the fluorescence signal reflected by the to-be-measured object received by the probe. The to-be-measured object is a local area of a human acupoint site or other site.

[0030] The control terminal 25 is connected with the excitation light path module, the optical fiber pre-calibration module and the signal detection module respectively, and is used for controlling the optical fiber pre-calibration module to collect holograms generated by beam interference of the reference light beam and the excitation light beam at equal intervals, calculating the output light field of the imaging optical fiber 12 according to the holograms, calculating the transmission matrix according to the output light field, calculating the input light field according to the transmission matrix and the target output light field, controlling the parameters of the excitation light beam generated by the excitation light path module according to the input light field, generating a diagnostic image according to the fluorescent signal, and controlling the laser generated by the excitation light path module according to the preset parameters and making the generated laser irradiate on the treatment site through the probe.

[0031] The multi-site laser acupuncture diagnosis and treatment integrated system of the embodiment of the application has three multi-site laser acupuncture diagnosis and treatment modes, including an optical fiber pre-calibration mode, an imaging diagnosis mode and a focused treatment mode.

[0032] When the multi-site laser acupuncture diagnosis and treatment mode is the optical fiber pre-calibration mode, the sample control module is removed; the transmission matrix of each multi-mode optical fiber in the probe is calculated respectively, wherein the calculation of the transmission matrix of one multi-mode optical fiber includes: locating the end face of the multi-mode optical fiber at the working distance of the second objective lens in the optical fiber pre-calibration module; preloading a pre-hologram in the light modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; controlling the ratio of the excitation light beam and the reference light beam generated by the excitation light path module, and phase-modulating the excitation light beam; controlling the optical fiber pre-calibration module to collect holograms generated by beam interference of the reference light beam and the excitation light beam at equal intervals; and calculating the output light field of the imaging optical fiber according to the holograms, so as to calculate the transmission matrix according to the output light field.

[0033] When the multi-site laser acupuncture diagnosis and treatment mode is the imaging diagnosis mode, time-sharing imaging is performed on multiple sites through a plurality of acupuncture needles and imaging optical fibers, wherein the imaging process of one site includes: loading a pre-hologram in the light modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; calculating the input light field according to the transmission matrix and the target output light field of the corresponding multi-mode optical fiber; controlling the parameters of the excitation light beam generated by the excitation light path module according to the input light field to make the multi-mode optical fiber form a focused light spot at the exit end to scan the measured object, collecting the fluorescent signal generated by the measured object scanned by the probe through the signal detection module, generating a diagnostic image according to the fluorescent signal, and thereby realizing imaging of the local area of the site of one multi-mode optical fiber. As an example, in this mode, the wavelength of the laser can be set to 473 nm, which is used to excite the endogenous fluorophore flavin adenine dinucleotide (FAD) to emit spontaneous fluorescent signals, thereby realizing imaging.

[0034] When the multi-site laser acupuncture diagnosis and treatment mode is the focused treatment mode, the laser generated by the excitation light path module is controlled according to the preset parameters and irradiated on the treatment site through the probe.

[0035] The multi-site laser acupuncture diagnosis and treatment integrated system of the embodiment of the present application calculates the transmission matrix of three optical fibers in the imaging optical fiber 12 through the optical fiber pre-calibration module, calculates the input light field according to the transmission matrix and the target output light field, controls the parameters of the excitation light beam generated by the excitation light path module according to the input light field, so that the exit end of the imaging optical fiber 12 forms a focused light spot scanning the object to be measured, generates a diagnostic image according to the fluorescent signal generated by the object to be measured, realizes high-resolution optical focusing of the acupoint site or other site local area, and realizes fast scanning of the focal point in the field of view to obtain a real-time clear and effective image, overcoming the defects that the existing invasive laser acupuncture technology has not been able to realize real-time imaging of the cells around the acupoint site or other site local area during the acupuncture stimulation process.

[0036] In an embodiment, the excitation light path module comprises a laser 1 for outputting a laser beam.

[0037] A first 4f system 2 is configured to receive the laser beam generated by the laser 1 and expand and collimate the laser beam.

[0038] A power adjustment module is configured to adjust the exit power of the laser beam and convert the laser beam into an excitation light beam and a reference light beam for output.

[0039] A second 4f system 5 is configured to expand and collimate the excitation light beam.

[0040] An optical modulation module 8 is configured to modulate the excitation light beam expanded and collimated by the second 4f system 5, so that the excitation light beam forms a focused light spot scanning the object to be measured at the exit end of the imaging optical fiber 12.

[0041] A third 4f system 9 is internally provided with a diaphragm 0902 configured to filter out the redundant diffraction order reflected light of the excitation light beam modulated by the optical modulation module 8, except for the target field information.

[0042] A coupling module is configured to couple the excitation light beam exiting from the third 4f system 9 into the imaging optical fiber 12 and separate the excitation light beam and the fluorescent signal.

[0043] Specifically, the laser 1 is a light source for outputting laser light, and the wavelength and intensity thereof are adjustable and have continuous / pulse two working modes.

[0044] The first 4f system 2 comprises a third lens 0201 and a fourth lens 0202, which are configured to receive the laser beam generated by the laser 1 and expand and collimate the laser beam.

[0045] The power adjustment module comprises a half-wave plate 3 and a first polarization beam splitter prism 4, the half-wave plate 3 is configured to adjust the polarization direction of the laser beam expanded and collimated by the first 4f system 2, and the first polarization beam splitter prism 4 is configured to cooperate with the half-wave plate 3 to adjust the exit power of the laser beam and convert the laser beam into an excitation light beam and a reference light beam for output.

[0046] The second 4f system 5 includes a fifth lens 0501 and a sixth lens 0502, configured to receive the excitation light beam after the output power is adjusted, and to expand and collimate the excitation light beam.

[0047] The first mirror 6 and the second mirror 7 are further arranged between the second 4f system 5 and the light modulation module 8, and are configured to reflect the excitation laser, receive the expanded and collimated excitation laser, and project the excitation laser to the light modulation module 8 at a preset angle.

[0048] The light modulation module 8 is configured to receive the reflected excitation light beam, and to modulate the laser beam according to the imported hologram switched at equal intervals of time, so as to form a series of focused light spots on the exit end of the imaging fiber 12 to scan the object.

[0049] The internal stop 0902 of the third 4f system 9 is arranged at the spectral plane in the third 4f system 9 to filter the diffraction orders containing the target field information, so as to filter out the redundant diffraction order reflected light in the excitation light beam modulated by the light modulation module 8, except for the diffraction order reflected light of the target field information.

[0050] The coupling module includes a dichroic mirror 10 and a first objective lens 11. The dichroic mirror 10 is configured to separate the excitation light beam and the fluorescent signal; and the first objective lens 11 is configured to couple the excitation light beam reflected by the third 4f system 9 and the dichroic mirror 10 in sequence into the imaging fiber 12.

[0051] In the embodiment of the present application, the wavefront shaping technology is introduced, the phase of the incident light is modulated by the light modulation module 8, the high-resolution optical focusing of the acupoint site or other site local area is realized, the fast scanning of the focal point in the field of view is realized, and the integrated imaging diagnosis and focusing treatment functions are realized.

[0052] In an embodiment, the optical fiber pre-calibration module includes:

[0053] The microscopic imaging system is configured to receive the excitation light beam emitted by the imaging fiber 12, and to perform end face imaging of the imaging fiber 12 according to the excitation light beam;

[0054] The light splitting prism 17 is configured to receive the reference light beam and the excitation light beam passing through the microscopic imaging system, and to cause the reference light beam and the excitation light beam to interfere to generate a hologram;

[0055] The reflection unit is configured to reflect the reference light beam to the light splitting prism 17;

[0056] The spatial pinhole filtering system is configured to filter stray light on the way of the reflection unit reflecting the reference light beam to the light splitting prism 17;

[0057] A surface detector 21 is used to collect the hologram and send the hologram to the control terminal 25.

[0058] Specifically, the microscopic imaging system comprises a second objective lens 13 and a tube lens 14, which are used to image the excitation light beam emitted by the imaging optical fiber 12.

[0059] The reflection unit comprises a third mirror 15 and a fourth mirror 16, both of which are used to reflect the laser light, receive the laser light beam output by the first polarization beam splitter prism 4 in the excitation light path module as a reference light beam, and project the reference light beam in the form of parallel light to the beam splitter prism 17.

[0060] The spatial pinhole filtering system comprises a third objective lens 18, a pinhole 19 and a first lens 20, which are sequentially placed between the third mirror 15 and the fourth mirror 16, and are used to receive the reference light beam and filter the stray light of the reference light beam.

[0061] The beam splitter prism 17 is placed behind the tube lens 14, the reflection end receives the reference light beam, and the transmission end receives the excitation light beam output by the microscopic imaging system, so that the excitation light beam and the reference light beam interfere to generate a hologram containing the amplitude information and phase information of the laser light beam. The surface detector 21 is a general detector, for example, a CMOS camera, which collects the hologram and sends the hologram to the control terminal 25.

[0062] The optical fiber pre-calibration module of the embodiment can calculate the output light field of the imaging optical fiber 12 through the collected hologram to calculate the transmission matrix, so that the input light field can be calculated according to the measured transmission matrix when the imaging diagnosis mode is performed, and the phase parameter of the excitation light beam generated by the excitation light path module is adjusted through the input light field to form a series of focused light spots at the exit end of the imaging optical fiber 12 to scan the object to be measured, realize high-resolution optical focusing of the acupoint site or other site local area, and obtain real-time clear and effective images, and improve the image clarity.

[0063] In an embodiment, the signal detection module comprises:

[0064] The second lens 22 is used to focus the fluorescence signal generated by the object to be measured to the point detector 23.

[0065] The point detector 23 is used to collect the fluorescence signal and send the fluorescence signal to the control terminal 25.

[0066] Specifically, the second lens 22 is a convex lens arranged at the transmission output end of the dichroic mirror 10, the fluorescence signal generated by the object to be measured is transmitted to the second lens 22 through the dichroic mirror 10, the second lens 22 receives the fluorescence signal and focuses it on the point detector 23, the point detector 23 adopts a device with high sensitivity, for example, can be a photomultiplier tube, the high-sensitivity point detector 23 collects the fluorescence signal and sends it to the control terminal 25.

[0067] In an embodiment, the multi-site laser acupuncture diagnosis and treatment integrated system further comprises a sample control module, the sample control module is used to carry and move the sample.

[0068] Specifically, the sample control module comprises a sample carrying sheet 24, a sample holder and an XY two-axis displacement table, the sample carrying sheet 24 and the sample holder are used to fix the sample such as cells, slices and living mice, the XY two-axis displacement table is used to manually or electrically move the sample, and is used to select the region of interest for imaging.

[0069] Through the sample control module, the sample position can be conveniently moved, and the fiber pre-calibration and diagnosis and treatment can be conveniently performed.

[0070] The multi-site laser acupuncture diagnosis and treatment integrated system of the embodiment of the application has three multi-site laser acupuncture diagnosis and treatment modes, as shown in the figure, including a fiber pre-calibration mode, an imaging diagnosis mode and a focused treatment mode. Figure 5

[0071] The fiber pre-calibration mode is used to measure the transmission matrix of the imaging fiber 12, and comprises: removing the sample control module; calculating the transmission matrix of the three fibers in the probe respectively, wherein the calculation of the transmission matrix of one fiber comprises: using the fiber holder to hold the proximal end of the multi-mode fiber, so that the end face is located at the working distance of the second objective lens in the fiber pre-calibration module; pre-loading the pre-hologram in the light modulation module to select the light source for providing uniform illumination on the distal end of the multi-mode fiber core; rotating the half-wave plate 3 to modulate the proportion of the excitation light beam and the reference light beam, so that the intensities of the signal light beam and the reference light beam are similar; using the Lee holographic method to load the Hadamard basis pattern with equal interval switching in the light modulation module 8 to phase modulate the excitation light beam generated by the laser light source; the general face detector 21 collects the hologram generated by the interference of the excitation light beam and the reference light beam at equal intervals, and calculates the output light field information of the imaging fiber 12 and the transmission matrix of the multi-mode fiber.

[0072] ​The imaging diagnosis mode includes: removing the optical fiber calibration module, and imaging multiple sites in time-sharing mode, and when the multi-mode optical fiber is provided with three, three sites can be imaged in time-sharing mode correspondingly, wherein the imaging process of one site includes: the light modulation module loads the pre-hologram to realize that a uniform light source is provided on the distal end of the core of one of the multi-mode optical fibers; the input light field is calculated according to the transmission matrix of the corresponding multi-mode optical fiber and the target output light field information, so that a series of focused light spots are formed on the exit end of one of the multi-mode optical fibers of the imaging optical fiber 12 to scan the object, generate a fluorescence signal, collect the fluorescence signal, and generate a diagnosis image according to the fluorescence signal, thereby realizing imaging of the local area of the site near the proximal end of one of the multi-mode optical fibers. The effect diagram of the light spot focusing is as shown in Figure 6 The phase of the incident light is modulated to realize high-resolution optical focusing of the acupoint site or other site local area, and the subsequent imaging quality is improved. As an example, in this mode, the wavelength of the laser can be set to 473 nm, which is used to excite the endogenous fluorophore flavin adenine dinucleotide (FAD) to emit spontaneous fluorescence signals to realize imaging.

[0073] The focused treatment mode includes: using the probe to acupuncture multiple sites of the part needing light treatment, and at the same time, three multi-mode optical fibers are also sent to three sites, and by controlling the working mode (continuous / pulse), wavelength and intensity of the laser 1, the laser is directly irradiated on the part at different wavelengths and different intensities in continuous / pulse mode, and by controlling the pre-hologram of the light modulation module, the three sites are irradiated in time-sharing or simultaneously. As an example, in this mode, the wavelength of the laser can be set to 808 nm or 810 nm, and studies have shown that in the PBM of mouse primary cortical neurons and human neuron cells, the use of 808 nm and 810 nm light irradiation can induce significant increases in calcium, ATP and MMP at lower energy, and reduce their production at higher energy, and at the same time, due to the up-regulation of cytochrome C oxidase, the neurons can be protected from the effects of excitotoxicity.

[0074] The multi-site laser acupuncture diagnosis and treatment integrated system of the embodiment of the application introduces wavefront shaping technology in the invasive laser acupuncture device, and by increasing the light modulation module 8 such as a digital micromirror array and a liquid crystal phase type spatial light modulator, the phase of the incident light is modulated to realize high-resolution optical focusing of the acupoint site or other site local area and rapid scanning of the focus in the field of view, and the integrated imaging diagnosis and focused treatment functions are realized.

[0075] The multi-site laser acupuncture diagnosis and treatment integrated system of the embodiment of the application can be applied to the following fields:

[0076] Reflecting real-time dynamic changes of acupoints and target organs during laser acupuncture stimulation is conducive to studying anatomical characteristics of acupoints and mechanisms of local acupoint stimulation by acupuncture and moxibustion.

[0077] The application can be applied to photobiomodulation, and can select a region of interest for multi-site time-sharing or simultaneous stimulation, accurately monitor and diagnose before, during and after surgery, evaluate treatment effect, and accurately select photobiomodulation treatment parameters according to the treatment effect.

[0078] The embodiment of the application also provides a multi-site laser acupuncture diagnosis and treatment integrated method applied to the multi-site laser acupuncture diagnosis and treatment integrated system in the above embodiment, and the method comprises the following steps:

[0079] In step S501, a multi-site laser acupuncture diagnosis and treatment mode is determined, wherein the multi-site laser acupuncture diagnosis and treatment mode comprises a fiber pre-calibration mode, an imaging diagnosis mode and a focused treatment mode.

[0080] In step S502, when the multi-site laser acupuncture diagnosis and treatment mode is the fiber pre-calibration mode, the sample control module is removed, and the transmission matrix of each multi-mode optical fiber in the probe is calculated, wherein the calculation of the transmission matrix of one multi-mode optical fiber comprises the following steps: the end face of the multi-mode optical fiber is located at the working distance of the second objective lens in the fiber pre-calibration module; a pre-hologram is pre-loaded in the light modulation module to provide a uniform illumination light source for the distal end of the multi-mode optical fiber core; the proportion of the excitation light beam and the reference light beam generated by the excitation light path module is controlled, and the excitation light beam is phase-modulated; the fiber pre-calibration module is controlled to collect holograms generated by the interference of the reference light beam and the excitation light beam at equal intervals, the output light field of the imaging optical fiber is calculated according to the holograms, and the transmission matrix is calculated according to the output light field.

[0081] In step S503, when the multi-site laser acupuncture diagnosis and treatment mode is the imaging diagnosis mode, time-sharing imaging is performed on a plurality of sites by a plurality of acupuncture needles and imaging optical fibers, wherein the imaging process of one site comprises the following steps: a pre-hologram is loaded in the light modulation module to provide a uniform illumination light source for the distal end of the multi-mode optical fiber core; the input light field is calculated according to the transmission matrix of the corresponding multi-mode optical fiber and the target output light field; the parameters of the excitation light beam generated by the excitation light path module are controlled according to the input light field, so that the multi-mode optical fiber forms a focused light spot at the exit end to scan the measured object; the fluorescence signal generated by the measured object scanned by the probe is collected by the signal detection module; and a diagnosis image is generated according to the fluorescence signal, so that the local area of the site of one multi-mode optical fiber is imaged.

[0082] In step S504, when the multi-site laser acupuncture diagnosis and treatment mode is the focusing treatment mode, the laser generated by the excitation light path module is controlled according to the preset parameters and is irradiated on the treatment site through the probe.

[0083] The multi-site laser acupuncture diagnosis and treatment integrated method provided by the embodiment of the present application calculates the transmission matrix of the imaging optical fiber 12 through the optical fiber pre-calibration module, calculates the input light field according to the transmission matrix and the target output light field, controls the parameters of the excitation light beam generated by the excitation light path module according to the input light field, so that the exit end of the imaging optical fiber 12 forms a focused light spot to scan the measured object, generates a diagnosis image according to the fluorescent signal generated by the measured object, realizes high-resolution optical focusing of the local area of the acupoint site or other site, and realizes the rapid scanning of the focal point in the field of view to obtain a real-time clear and effective image, overcoming the defect that the existing invasive laser acupuncture technology has not been able to realize real-time imaging of the cells around the acupoint site or the local area of other sites in the acupuncture stimulation process.

[0084] Although the embodiments of the present application are described in conjunction with the drawings, various modifications and changes can be made by those skilled in the art without departing from the spirit and scope of the present application, and such modifications and changes fall within the scope defined by the appended claims.

Claims

1. A multi-site laser acupuncture diagnosis and treatment integrated system, characterized in that, The application relates to a kind of optical imaging diagnosis device and method. Probe, including several acupuncture needles and imaging optical fibers, the end of the imaging optical fiber includes at least one multi-mode optical fiber, each multi-mode optical fiber is arranged in the inside of corresponding acupuncture needle respectively; Optical imaging diagnosis device, including excitation light path module, optical fiber pre-calibration module and signal detection module, the excitation light path module is used to generate excitation light beam and reference light beam and focus the excitation light beam to the multi-mode optical fiber, the optical fiber pre-calibration module is used to collect the hologram generated by the interference of the reference light beam and the excitation light beam; The signal detection module is used to collect the fluorescence signal generated by the object to be measured scanned by the probe;Wherein, the signal detection module includes: second lens, for focusing the fluorescence signal generated by the object to be measured to point detector;The point detector is used to collect the fluorescence signal and send the fluorescence signal to the control terminal; Control terminal, respectively and the excitation light path module, optical fiber pre-calibration module and signal detection module are connected, for controlling the optical fiber pre-calibration module and the like interval to collect the hologram generated by the interference of reference light beam and the excitation light beam, the output light field of the imaging optical fiber is calculated according to the hologram, so as to calculate the transmission matrix according to the output light field, the input light field is calculated according to the transmission matrix and the target output light field, the parameters of the excitation light beam generated by the excitation light path module are controlled according to the input light field, the diagnosis image is generated according to the fluorescence signal, and the laser generated by the excitation light path module is controlled according to the preset parameter and the laser generated by the excitation light path module is irradiated on the treatment site through the probe.

2. The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 1, characterized in that, The excitation light path module includes: Laser, for outputting laser beam; First 4f system, for receiving the laser beam generated by the laser, and expanding and collimating the laser beam; Power regulation module, for adjusting the exit power of the laser beam, and converting the laser beam into excitation light beam and reference light beam output; Second 4f system, for expanding and collimating the excitation light beam; Light modulation module, for modulating the excitation light beam after the expansion and collimation of the second 4f system, so that the excitation light beam forms a focused spot at the exit end of the imaging optical fiber and scans the object to be measured; Third 4f system, provided with a diaphragm inside, for filtering out the redundant diffraction order reflected light of the excitation light beam modulated by the light modulation module, except the target field information; Coupling module, for coupling the excitation light beam emitted by the third 4f system into the imaging optical fiber, and separating the excitation light beam and the fluorescence signal.

3. The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 2, characterized in that, The power regulation module includes: Half-wave plate, for adjusting the polarization direction of the laser beam after the expansion and collimation of the first 4f system; First polarization beam splitter prism, for adjusting the exit power of the laser beam in cooperation with the half-wave plate, and converting the laser beam into excitation light beam and reference light beam output.

4. The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 2, characterized in that, The coupling module includes: Dichroic mirror, for separating the excitation light beam and the fluorescence signal; First objective lens, for coupling the excitation light beam reflected by the third 4f system and the dichroic mirror in turn into the imaging optical fiber.

5. The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 1, characterized in that, The optical fiber pre-calibration module comprises: a microscopic imaging system configured to receive the excitation light beam emitted by the imaging optical fiber and perform end face imaging on the imaging optical fiber according to the excitation light beam; a beam splitting prism configured to receive the reference light beam and the excitation light beam passing through the microscopic imaging system and cause the reference light beam and the excitation light beam to interfere to generate a hologram; a reflection unit configured to reflect the reference light beam to the beam splitting prism; a spatial pinhole filtering system configured to filter stray light on the way of the reflection unit reflecting the reference light beam to the beam splitting prism; a surface detector configured to collect the hologram and send the hologram to the control terminal. 6.The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 2, characterized in that, The optical modulation module is a digital micromirror array or a liquid crystal phase spatial light modulator. 7.The multi-site laser acupuncture diagnosis and treatment integrated system according to claim 1, characterized in that, Further comprising: a sample control module configured to carry and move a sample.

8. The multi-site laser acupuncture diagnosis and treatment integrated system according to any one of claims 1 to 7, characterized in that, The multi-site laser acupuncture diagnosis and treatment integrated system is applied to: determining a multi-site laser acupuncture diagnosis and treatment mode, wherein the multi-site laser acupuncture diagnosis and treatment mode comprises an optical fiber pre-calibration mode, an imaging diagnosis mode and a focused treatment mode; when the multi-site laser acupuncture diagnosis and treatment mode is the optical fiber pre-calibration mode, removing the sample control module; calculating a transmission matrix of each multi-mode optical fiber in the probe, wherein the calculation of the transmission matrix of one multi-mode optical fiber comprises: locating the end face of the multi-mode optical fiber at the working distance of the second objective lens in the optical fiber pre-calibration module; pre-loading a pre-hologram in the optical modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; controlling the proportion of the excitation light beam and the reference light beam generated by the excitation light path module, and phase modulating the excitation light beam; controlling the optical fiber pre-calibration module to collect holograms generated by the interference of the reference light beam and the excitation light beam at equal intervals, calculating the output light field of the imaging optical fiber according to the holograms, and calculating the transmission matrix according to the output light field; when the multi-site laser acupuncture diagnosis and treatment mode is the imaging diagnosis mode, performing time-sharing imaging on multiple sites through a plurality of acupuncture needles and imaging optical fibers, wherein the imaging process of one site comprises: loading a pre-hologram in the optical modulation module to provide a uniform light source for the distal end of the core of one multi-mode optical fiber; calculating an input light field according to the transmission matrix of the corresponding multi-mode optical fiber and a target output light field; controlling the parameters of the excitation light beam generated by the excitation light path module according to the input light field to form a focused light spot at the exit end of the multi-mode optical fiber to scan the object to be measured; collecting a fluorescence signal generated by the object to be measured scanned by the probe through the signal detection module; generating a diagnosis image according to the fluorescence signal, thereby realizing imaging of the local area of the site of one multi-mode optical fiber; when the multi-site laser acupuncture diagnosis and treatment mode is the focused treatment mode, controlling the laser generated by the excitation light path module according to the preset parameters and irradiating the generated laser on the treatment site through the probe.

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