Traditional Chinese medicine composition for treating lithiasis in urinary system as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for treating urinary calculus, which comprises the following components in parts by weight: 4-12 parts of extract A; 2-6 parts of an extract B; 10 to 20 parts of an extract C; 3-7 parts of an extract D; the extract A is mixed powder of 3 parts of vinegar-processed rhizoma corydalis and 4 parts of scalded endothelium corneum gigeriae galli; the extract B is a cyclodextrin inclusion compound of water vapor distilled volatile oil of 2 parts of radix aucklandiae; the extract C is a refined product obtained by mixing 2 parts of radix aucklandiae dregs and distillate obtained after steam distillation, 10 parts of fried cowherb seed, 4 parts of radix salviae miltiorrhizae, 6 parts of polygonum aviculare, 5 parts of dianthus superbus, 3 parts of radix achyranthis bidentatae and 4 parts of lygodium japonicum and then performing water extraction and alcohol precipitation; the extract D is an ethanol eluate obtained after 10 parts of lysimachia christinae hance is subjected to reflux extraction and passes through macroporous adsorption resin. The traditional Chinese medicine composition is used as a medicine in an extract form, and is relatively complete in active part, clear in effective component, scientific in effective component proportion, clear in material basis, small in taking dosage, free of cane sugar in a formula and wide in applicable crowd.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for treating urinary calculi, a preparation method thereof and application thereof. Background Art

[0002] Urinary stones are a common urinary tract disease, including kidney stones, bladder stones, and ureteral stones. The causes are closely related to gender, age, occupation, diet, and lifestyle. The incidence is increasing due to high work pressure, limited exercise, and low water intake. In Traditional Chinese Medicine, this condition is known as "stone stranguria," "sand stranguria," "lumbar pain," and "blood stranguria." Treatment of urinary stones with traditional Chinese medicine, which clears heat and dampness, is safe and effective, avoiding surgical trauma.

[0003] Currently, traditional Chinese medicine compositions for urinary stones are not suitable for special populations due to the backward traditional preparation methods, limited extraction ingredients, low content of effective ingredients, slow onset of drug action, and the presence of sucrose in the preparation method. At the same time, the single dose is relatively large, the compliance is poor, the specificity is not strong, and the indications are not clear, resulting in uneven clinical efficacy of similar drugs. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition for treating urinary stones, a preparation method and application thereof, wherein the traditional Chinese medicine composition has higher pharmaceutical efficacy.

[0005] To achieve the above object, the present invention provides a traditional Chinese medicine composition for treating urinary calculi, comprising, by weight:

[0006] 4-12 parts of extract A;

[0007] Extract B 2-6 parts;

[0008] 10-20 parts of extract C;

[0009] Extract D 3-7 parts;

[0010] The extract A is a mixed powder of 3 parts of vinegared Corydalis yanhusuo and 4 parts of boiled chicken's gizzard lining;

[0011] The extract B is a cyclodextrin inclusion compound of 2 parts of steam distilled volatile oil from costus root;

[0012] The extract C is a mixture of the residue and distillate of 2 parts of costus root, 10 parts of stir-fried angelica root, 4 parts of salvia miltiorrhiza, 6 parts of polyanthus scutellariae, 5 parts of dianthus, 3 parts of achyranthes bidentata, and 4 parts of lygodia scabra, which are then extracted with water and precipitated with alcohol.

[0013] The extract D is the ethanol eluate of 10 parts of Lysimachia chinensis after reflux extraction and passing through a macroporous adsorption resin.

[0014] Preferably, the Chinese medicine composition for treating urinary calculi comprises:

[0015]

[0016] In some specific embodiments of the present invention, the contents (by weight) of extract A, extract B, extract C, and extract D in the traditional Chinese medicine composition for treating urinary calculi are as follows: 4, 2, 10, 3; 4, 4, 15, 5; 4, 6, 20, 7; 8, 2, 15, 7; 8, 4, 20, 3; 8, 6, 10, 5; 12, 2, 20, 5; 12, 4, 10, 7;

[0025] 12, 6, 20, 3.

[0026] Most preferably, the Chinese medicine composition for treating urinary calculi comprises:

[0027]

[0028] The test results show that the Chinese medicine composition of the most preferred components can reduce kidney mass, urine calcium concentration, and blood calcium concentration to the greatest extent, and has the most obvious analgesic effect.

[0029] The present invention provides a method for preparing the above-mentioned traditional Chinese medicine composition for treating urinary calculi, comprising the following steps:

[0030] A) Ultrafinely pulverize 3 parts of vinegar-corydalis yanhusuo and 4 parts of blanched chicken's gizzard lining into fine powder to obtain extract A;

[0031] B) Grinding 2 parts of costus root into coarse powder, and extracting by steam distillation to obtain volatile oil, medicinal residue and distillate;

[0032] The volatile oil, β-cyclodextrin and water are mixed to obtain an inclusion compound, which is dried to obtain extract B;

[0033] C) taking the residue and distillate of the above extract B, 10 parts of stir-fried angelica sinensis, 4 parts of salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, mixing them, and then extracting with water and precipitating with alcohol to obtain extract C;

[0034] D) taking 10 parts of Lysimachia sibiricum, pulverizing and refluxing extraction, filtering, the filtrate was concentrated and passed through a macroporous adsorption resin, eluted with water, the eluate was discarded, and then eluted with ethanol, the eluate was collected, and concentrated under reduced pressure to obtain an extract to obtain an extract D;

[0035] E) mixing the extract A, extract B, extract C and extract D to obtain a traditional Chinese medicine composition for treating urinary calculi.

[0036] The present invention has no special limitation on the vinegar-corydalis yanhusuo, which can be commercially available vinegar-corydalis yanhusuo or self-prepared. It is preferably prepared by vinegar-roasting method. More preferably, the yanhusuo is fried dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213) and cut into thick slices.

[0037] The present invention has no special limitation on the scalded chicken's gizzard lining, and the scalded chicken's gizzard lining can be commercially available scalded chicken's gizzard lining or self-prepared. Preferably, the scalding method is used for preparation. More preferably, the chicken's gizzard lining is fried until it swells according to the scalding method (General Chapter 0213 of the Chinese Pharmacopoeia).

[0038] In the above step B), the material-liquid ratio of steam distillation is preferably 1:(6-10), more preferably 1:8.

[0039] The steam distillation time is preferably 4 to 10 hours, more preferably 6 hours.

[0040] The amount of β-cyclodextrin added is preferably 3 to 8 times the mass of the volatile oil, more preferably 6 times.

[0041] The amount of water added is preferably 10 to 15 times the mass of the volatile oil, more preferably 12 times.

[0042] Preferably, the volatile oil, β-cyclodextrin and water are mixed, and then ground with a colloid mill and filtered.

[0043] The drying is preferably freeze-drying.

[0044] The present invention has no special limitation on the stir-fried angelica dahurica, and it can be commercially available stir-fried angelica dahurica or self-prepared. It is preferably stir-fried, and more preferably stir-fried according to the stir-frying method (Chinese Pharmacopoeia General Chapter 0213) until most of the white flowers burst.

[0045] In the step C), the amount of water used in the water extraction is preferably 4 to 10 times the total mass of the drug, more preferably 6 times.

[0046] The water extraction is preferably performed 1 to 3 times, more preferably 1 to 2 times.

[0047] After water extraction, the solution is filtered and the filtrate is concentrated to obtain a clear paste.

[0048] The relative density of the clear paste is preferably 1.10-1.20 (80° C.).

[0049] After obtaining the clear paste, it is subjected to alcohol precipitation.

[0050] The alcohol precipitation preferably uses ethanol.

[0051] The amount of ethanol used is preferably 60 wt% to 80 wt% of the clear paste obtained after water extraction, and more preferably 60 wt%.

[0052] The alcohol precipitation is specifically:

[0053] Add ethanol to dissolve the clear paste obtained by water extraction, let it stand and then filter it, recover ethanol from the filtrate, and concentrate it to a thick paste with a relative density of 1.20-1.30 (80°C).

[0054] Preferably, the thick paste is dried.

[0055] The drying is preferably carried out under reduced pressure.

[0056] The temperature of the reduced pressure drying is preferably 60 to 80°C, more preferably 70 to 80°C.

[0057] In the step D), the reflux extraction is preferably performed 1 to 3 times, more preferably 1 to 2 times.

[0058] Preferably, the filtrate is concentrated as follows:

[0059] The filtrate is concentrated to 1.0-1.2 g of crude drug per ml.

[0060] The macroporous adsorption resin is preferably D101 macroporous adsorption resin.

[0061] The amount of water used for elution is preferably 2 to 4 times the volume of the column.

[0062] The amount of ethanol used in the ethanol elution is preferably 5 to 8 times the column volume.

[0063] The concentration of the ethanol is preferably 50% to 70%.

[0064] The decompression concentration to obtain an extract preferably comprises:

[0065] Concentrate to an extract with a relative density of 1.24-1.26 (80°C).

[0066] The above-mentioned traditional Chinese medicine composition may further include pharmaceutically acceptable adjuvants, including but not limited to flavoring agents, stabilizers, viscosity enhancers, etc.

[0067] In some specific embodiments of the present invention, the traditional Chinese medicine composition further comprises xylitol and dextrin.

[0068] The present invention provides the use of the above-mentioned traditional Chinese medicine composition for treating urinary calculi or the traditional Chinese medicine composition for treating urinary calculi according to any one of claims 1 to 3 prepared by the above-mentioned preparation method in preparing a drug for treating or alleviating urinary calculi.

[0069] Compared with the prior art, the present invention provides a traditional Chinese medicine composition for treating urinary system stones, which comprises, by weight, 4 to 12 parts of extract A; 2 to 6 parts of extract B; 10 to 20 parts of extract C; and 3 to 7 parts of extract D. The extract A is a mixed powder of 3 parts of vinegared Corydalis yanhusuo and 4 parts of scalded chicken's gizzard lining; the extract B is a cyclodextrin inclusion compound of steam-distilled volatile oil from 2 parts of costus root; the extract C is a refined product obtained by water extraction and alcohol precipitation after mixing dregs and distillate of steam-distilled costus root from 2 parts of costus root, 10 parts of stir-fried angelica sinensis, 4 parts of salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistortae, 3 parts of Achyranthes bidentatae, and 4 parts of Lygodium japonicum; and the extract D is an ethanol eluate obtained by reflux extraction of 10 parts of Lycopodiella cuneata and passing it through a macroporous adsorption resin.

[0070] The present invention is based on the prescription and compatibility of Shenshitong granules. The various medicinal materials in the prescription are enriched, separated, and purified to obtain different extraction fractions, which are then recombined to form a new prescription. The prescription is used as an extract, has relatively complete active parts, clear effective ingredients, a scientific ratio of effective components, a clear material basis, a small dosage, and is sucrose-free, making it suitable for a wide range of people. DETAILED DESCRIPTION

[0071] To further illustrate the present invention, the following is a detailed description of the embodiments. However, it should be understood that these descriptions are only for the purpose of further illustrating the features and advantages of the present invention, rather than for limiting the scope of the invention.

[0072] All raw materials of the present invention are not particularly limited in their sources and can be purchased from the market or prepared according to conventional methods well known to those skilled in the art.

[0073] Example 1

[0074] 1. Preparation of Extract A:

[0075] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 350-mesh sieve to obtain Extract A.

[0076] 2. Preparation of Extract B:

[0077] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0078] 3. Preparation of Extract C:

[0079] Take 10 parts of fenugreek and stir-fry them according to the stir-fry method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry fenugreek, 4 parts of salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.10 (80°C), add appropriate amount of ethanol to make the alcohol content reach 60%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.20 (80°C), and dry under reduced pressure at 70°C to obtain Chinese medicine extract C.

[0080] 4. Preparation of Extract D:

[0081] Get 10 parts of Lysimachia scutellariae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to 1.0g crude drug every milliliter, by D101 type macroporous adsorption resin, with 2-4 times of column volume of water wash-out, discard eluent, then use 5 times of column volume of 70% ethanol wash-out, collect eluent, concentrate under reduced pressure, be concentrated to a thick paste with relative density 1.24 (80 ℃), obtain Chinese medicine extract D.

[0082] 5. Preparation of Chinese medicine composition:

[0083] Take 4 parts of Chinese herbal medicine extract A, 2 parts of Chinese herbal medicine extract B, 10 parts of Chinese herbal medicine extract C, and 3 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0084] Example 2

[0085] 1. Preparation of Extract A:

[0086] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 350-mesh sieve to obtain Extract A.

[0087] 2. Preparation of Extract B:

[0088] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0089] 3. Preparation of Extract C:

[0090] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.20 (80°C), add appropriate amount of ethanol to make the alcohol content reach 70%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.30 (80°C), and dry under reduced pressure at 80°C to obtain Chinese medicine extract C.

[0091] 4. Preparation of Extract D:

[0092] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 3 times of column volumes of water, discarded eluent, then eluted with 6 times of column volumes of 60% ethanol, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.25 (80°C), to obtain Chinese medicine extract D.

[0093] 5. Preparation of Chinese medicine composition:

[0094] Take 4 parts of Chinese herbal medicine extract A, 4 parts of Chinese herbal medicine extract B, 15 parts of Chinese herbal medicine extract C, and 5 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0095] Example 3

[0096] 1. Preparation of Extract A:

[0097] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 400-mesh sieve to obtain Extract A.

[0098] 2. Preparation of Extract B:

[0099] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0100] 3. Preparation of Extract C:

[0101] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.15 (80°C), add appropriate amount of ethanol to make the alcohol content reach 80%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate it to a thick paste with a relative density of 1.25 (80°C), and dry under reduced pressure at 80°C to obtain Chinese medicine extract C.

[0102] 4. Preparation of Extract D:

[0103] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 4 times of column volumes of water, discarded eluent, then eluted with 50% ethanol with 7 times of column volumes, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.24 (80°C), to obtain Chinese medicine extract D.

[0104] 5. Preparation of Chinese medicine composition:

[0105] Take 4 parts of Chinese herbal medicine extract A, 6 parts of Chinese herbal medicine extract B, 20 parts of Chinese herbal medicine extract C, and 7 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0106] Example 4

[0107] 1. Preparation of Extract A:

[0108] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 400-mesh sieve to obtain Extract A.

[0109] 2. Preparation of Extract B:

[0110] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0111] 3. The preparation method of extract C is as follows:

[0112] Take 10 parts of fenugreek and stir-fry them according to the stir-fry method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry fenugreek, 4 parts of salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.10 (80°C), add appropriate amount of ethanol to make the alcohol content reach 60%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.20 (80°C), and dry under reduced pressure at 75°C to obtain Chinese medicine extract C.

[0113] 4. Preparation of Extract D:

[0114] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 2 times of column volumes of water, discarded eluent, then eluted with 50% ethanol with 8 times of column volumes, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.26 (80°C), to obtain Chinese medicine extract D.

[0115] 5. Preparation of Chinese medicine composition:

[0116] Take 8 parts of Chinese herbal extract A, 2 parts of Chinese herbal extract B, 15 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0117] Example 5

[0118] 1. Preparation of Extract A:

[0119] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 350-mesh sieve to obtain Extract A.

[0120] 2. Preparation of Extract B:

[0121] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0122] 3. Preparation of Extract C:

[0123] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.20 (80°C), add appropriate amount of ethanol to make the alcohol content reach 60%, let stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.30 (80°C), and dry under reduced pressure at 70°C to obtain Chinese medicine extract C.

[0124] 4. Preparation of Extract D:

[0125] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, first 2.5 hours, second 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 3 times of column volumes of water, discarded eluent, then eluted with 60% ethanol with 8 times of column volumes, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.24 (80°C), to obtain Chinese medicine extract D.

[0126] 5. Preparation of stone Chinese medicine composition:

[0127] Take 8 parts of Chinese herbal extract A, 4 parts of Chinese herbal extract B, 20 parts of Chinese herbal extract C, and 3 parts of Chinese herbal extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0128] Example 6

[0129] 1. Preparation of Extract A:

[0130] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 400-mesh sieve to obtain Extract A.

[0131] 2. Preparation of Extract B:

[0132] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0133] 3. Preparation of Extract C:

[0134] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.18 (80°C), add appropriate amount of ethanol to make the alcohol content reach 75%, let stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.20 (80°C), and dry under reduced pressure at 80°C to obtain Chinese medicine extract C.

[0135] 4. Preparation of Extract D:

[0136] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 4 times of column volumes of water, discarded eluent, then eluted with 7 times of column volumes of 70% ethanol, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.26 (80°C), to obtain Chinese medicine extract D.

[0137] 5. Preparation of Chinese medicine composition:

[0138] Take 8 parts of Chinese herbal extract A, 6 parts of Chinese herbal extract B, 10 parts of Chinese herbal extract C, and 5 parts of Chinese herbal extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0139] Example 7

[0140] 1. Preparation of Extract A:

[0141] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 350-mesh sieve to obtain Extract A.

[0142] 2. Preparation of Extract B:

[0143] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0144] 3. Preparation of Extract C:

[0145] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.20 (80°C), add appropriate amount of ethanol to make the alcohol content reach 60%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.20 (80°C), and dry under reduced pressure at 80°C to obtain Chinese medicine extract C.

[0146] 4. Preparation of Extract D:

[0147] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 4 times of column volumes of water, discarded eluent, then eluted with 5 times of column volumes of 50% ethanol, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.24 (80°C), to obtain Chinese medicine extract D.

[0148] 5. Preparation of Chinese medicine composition:

[0149] Take 12 parts of Chinese herbal medicine extract A, 2 parts of Chinese herbal medicine extract B, 20 parts of Chinese herbal medicine extract C, and 5 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0150] Example 8

[0151] 1. Preparation of Extract A:

[0152] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 400-mesh sieve to obtain Extract A.

[0153] 2. Preparation of Extract B:

[0154] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0155] 3. Preparation of Extract C:

[0156] Take 10 parts of Vaccaria segetalis and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.10 (80°C), add appropriate amount of ethanol to make the alcohol content reach 80%, let stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.30 (80°C), and dry under reduced pressure at 80°C to obtain Chinese medicine extract C.

[0157] 4. Preparation of Extract D:

[0158] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, the first time 2.5 hours, the second time 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 3 times of column volumes of water, discarded eluent, then eluted with 6 times of column volumes of 60% ethanol, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.25 (80°C), to obtain Chinese medicine extract D.

[0159] 5. Preparation of Chinese medicine composition:

[0160] Take 12 parts of Chinese herbal extract A, 4 parts of Chinese herbal extract B, 10 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000 g of granules.

[0161] Example 9

[0162] 1. Preparation of Extract A:

[0163] Take 3 parts of Corydalis yanhusuo, stir-fry until dry according to the vinegar-roasting method (Chinese Pharmacopoeia General Chapter 0213), and cut into thick slices. Take 4 parts of chicken gizzard lining, stir-fry according to the blanching method (Chinese Pharmacopoeia General Chapter 0213) until swollen; ultrafinely grind into fine powder, and pass through a 350-mesh sieve to obtain Extract A.

[0164] 2. Preparation of Extract B:

[0165] Take 2 parts of costus root, grind it into coarse powder, add it into water with a solid-liquid ratio of 1:8, and extract it with steam distillation for 6 hours. Collect the volatile oil, add 6 times the amount of β-cyclodextrin and 12 times the amount of water, grind it with a colloid mill for 1 hour, filter it, and freeze-dry the inclusion compound to obtain Chinese medicine extract B.

[0166] 3. Preparation of Extract C:

[0167] Take 10 parts of fenugreek and stir-fry them according to the stir-frying method (General Chapter 0213 of the Chinese Pharmacopoeia) until most of the white flowers burst; take the residue and distillate of extract B, stir-fry fenugreek, 4 parts of salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus bistorta, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, add 6 times the amount of water, soak for 30 minutes, and extract under hot reflux twice, the first time for 2.5 hours and the second time for 1.5 hours, filter, and concentrate the filtrate to a clear paste with a relative density of 1.20 (80°C), add appropriate amount of ethanol to make the alcohol content reach 70%, let it stand for 24 hours, filter, recover ethanol from the filtrate, and concentrate to a thick paste with a relative density of 1.20-1.30 (80°C), and dry under reduced pressure at 75°C to obtain Chinese medicine extract C.

[0168] 4. Preparation of Extract D:

[0169] Get 10 parts of Herba Lysimachiae, add 10 times of water, soak for 30 minutes, be ground into coarse powder, hot reflux extraction 2 times, first 2.5 hours, second 1.5 hours, filter, the filtrate is concentrated to every milliliter of 1.0g crude drug, by D101 type macroporous adsorption resin, eluted with 3 times of column volumes of water, discarded eluent, then eluted with 60% ethanol with 8 times of column volumes, collected eluent, concentrated under reduced pressure, concentrated to a thick paste with a relative density of 1.24 (80°C), to obtain Chinese medicine extract D.

[0170] 5. Preparation of Chinese medicine composition:

[0171] Take 12 parts of Chinese herbal medicine extract A, 6 parts of Chinese herbal medicine extract B, 20 parts of Chinese herbal medicine extract C, and 3 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0172] Comparative Example 1

[0173] The preparation methods of Extract A, Extract B, Extract C, and Extract D are the same as those in Example 1.

[0174] Take 3 parts of Chinese herbal medicine extract A, 1 part of Chinese herbal medicine extract B, 9 parts of Chinese herbal medicine extract C, and 2 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000g of granules.

[0175] Comparative Example 2

[0176] The preparation methods of Extract A, Extract B, Extract C, and Extract D are the same as those in Example 1.

[0177] Take 13 parts of Chinese herbal medicine extract A, 7 parts of Chinese herbal medicine extract B, 21 parts of Chinese herbal medicine extract C, and 8 parts of Chinese herbal medicine extract D, add appropriate amounts of xylitol and dextrin, mix well, and make 1000 g of granules.

[0178] Efficacy trials

[0179] In the following experiments, the preparation method of the original process group is as follows:

[0180] Put 4 parts of Lycopodiella and 10 parts of Vaccaria segetalis (fried) into a cloth bag, add water and decoct twice with 10 parts of Herba Lysimachiae, 6 parts of Radix Polygoni Multiflori, 3 parts of Corydalis yanhusuo (made with vinegar), 4 parts of Gallus galli (scalded), 4 parts of Salvia miltiorrhiza, 2 parts of costus root, 5 parts of Dianthus bistorta, and 3 parts of Achyranthes bidentata, the decoctions are combined, filtered, and concentrated under reduced pressure to a thick paste with a relative density of 1.20-1.25 (75-80°C). Add appropriate amounts of sucrose and dextrin, mix well, and make 1000g of granules.

[0181] Compare the pharmacodynamics of experimental groups with different formulation ratios:

[0182] 1. Therapeutic effect on renal stones in rats

[0183] 1.1 Experimental Animals

[0184] 140 Wistar rats, male, 180-220 g.

[0185] 1.2 Reagents

[0186] Ethylene glycol, manufacturer: Sigma-Aldrich (Shanghai); ammonium chloride, manufacturer: Sigma-Aldrich (Shanghai); calcium gluconate injection, manufacturer: Harbin Pharmaceutical Group Sanjin Pharmaceutical Co., Ltd.

[0187] 1.3 Experimental methods

[0188] 140 Wistar rats were randomly divided into 14 groups, namely, blank group, model group, Example 1-9 group, Comparative Example 1-2 group, and original process group, with 10 rats in each group. Except for the blank group, all other groups were given 1% ethylene glycol in drinking water and 2% ammonium chloride orally (2 mL / rat) for 28 consecutive days to establish the model. Each group was orally administered with the corresponding test substance at a volume of 10 ml / kg for 28 consecutive days.

[0189] 1.4 Detection indicators

[0190] After the administration, urine was collected for 24 hours to determine the urine calcium content (o-cresolphthalein complex spectrophotometry); blood was collected from the retroorbital vein, centrifuged at 3000 rpm / min for 15 minutes, and the blood calcium concentration was measured by o-cresolphthalein complex spectrophotometry; after blood collection, the rats were killed by cervical dislocation, and both kidneys were removed to observe the appearance of organ tissues and stone formation, and the organ weights were weighed.

[0191] 1.5 Experimental Results

[0192] Compared with the blank group, the organ mass, blood calcium concentration and urine calcium concentration of the model group were significantly increased (P < 0.001), indicating that the animal model was successfully established. Compared with the model group, the organ mass of the original process group and Example 2, 3, 4, 5, 6, 8, and 9 groups was significantly reduced (P < 0.05); except for the comparative example 1 and 2 groups, the blood calcium and urine calcium concentrations of the other groups were significantly reduced (P < 0.05, P < 0.01, P < 0.001). Compared with the original process group, the kidney mass of Examples 4 and 5 was significantly reduced (P < 0.05); there was no significant difference in blood calcium and urine calcium concentrations in each group (P > 0.05). Compared with the comparative example 1 group, the kidney mass of Example 2, 3, 4, 5, 6, 8, and 9 groups was significantly reduced (P < 0.05, P < 0.01); the blood calcium concentration of Example 2, 3, 4, 5, 8, and 9 groups was significantly reduced (P < 0.05, P < 0.01, P < 0.001); the urine calcium concentration of Example 5 and 7 groups was significantly reduced (P < 0.05). Compared with the comparative example 2 group, the kidney mass of Example 3, 4, 5, 6, and 9 groups was significantly reduced (P < 0.05, P < 0.01, P < 0.001); the blood calcium concentration of Example 3 and 5 groups was significantly reduced (P < 0.05, P < 0.01); the urine calcium concentration of Example 5 group was significantly reduced (P> 0.05). The results show that the therapeutic effect of Example 5 on kidney stones is better than that of other groups, as shown in Table 1.

[0193] Table 1 Comparison of kidney mass, blood calcium and urine calcium in rats of each group (x±s, n=10)

[0194]

[0195]

[0196] Note: Compared with the blank control group, ### P<0.001; compared with the model control group, * P<0.05, ** P<0.01, *** P<0.001; compared with the original process group, △P<0.05; compared with the comparative example 1 group, ※ P<0.05, ※※ P<0.01, ※※※ P<0.001; compared with control group 2, □ P<0.05, □□ P<0.01, □□□ P<0.001.

[0197] 2 Analgesia experiment in mice

[0198] 2.1 Experimental Animals

[0199] ICR mice, male, 18-22 g, 140 mice.

[0200] 2.2 Reagents

[0201] Acetic acid, manufacturer: Tianjin Zhongtian Chemicals Co., Ltd.

[0202] 2.3 Experimental methods

[0203] 140 ICR mice were randomly divided into 14 groups, namely blank group, model group, Example 1-9 group, Comparative Example 1-2 group, and original process group, with 10 mice in each group. Each drug-dosing group was gavage-administered with the corresponding drug once a day for 3 consecutive days, and the blank group was given an equal amount of drinking water. 60 minutes after the last administration, all groups of mice except the blank group were intraperitoneally injected with 0.6% glacial acetic acid solution, 0.2 ml / mouse, to induce a writhing reaction in the mice. The blank group mice were intraperitoneally injected with an equal volume of normal saline. This reaction occurred most frequently within 15 minutes after injection, so the number of writhing reactions within 15 minutes after injection and the time of writhing reactions were used as detection indicators, and the inhibition rate of the drug on the writhing reaction was calculated.

[0204] Inhibition rate = (average number of writhing times in the model group - average number of writhing times in the drug-treated group) / average number of writhing times in the model group × 100%

[0205] 2.4 Experimental Results

[0206] Compared with the blank group, the writhing latency and number of writhings in the model group showed significant changes, and were statistically significant (P < 0.001). Compared with the model group, except for comparative example groups 1 and 2, the writhing latency of all other groups was prolonged to varying degrees, and was statistically significant (P < 0.05, P < 0.01, P < 0.001); the number of writhings in the original process group and examples 1, 2, 5, 6, 7, and 8 groups was significantly reduced (P < 0.05, P < 0.01, P < 0.001). Compared with the original process group, the writhing latency of examples 4, 5, and 6 groups was significantly prolonged (P < 0.05, P < 0.001); the number of writhings in example 3 group was significantly increased (P < 0.05). Compared with the comparative example 1 group, except for the Example 2 and 3 groups, the writhing latency of the remaining groups was significantly prolonged (P < 0.05, P < 0.01, P < 0.001); the number of writhing times in the Example 1 and 5 groups was significantly reduced (P < 0.05, P < 0.01). Compared with the comparative example 2 group, except for the Example 3 group, the writhing latency of the remaining groups was significantly prolonged (P < 0.05, P < 0.01, P < 0.001); the number of writhing times in the Example 1 and 5 groups was significantly reduced (P < 0.05). The comprehensive writhing inhibition rate results show that the analgesic effect of Example 5 is better than that of the other groups, as shown in Table 2.

[0207] Table 2 Comparison of analgesic effect in mice of each group (x±s, n=10)

[0208]

[0209] Note: Compared with the blank group, □□□ P<0.001; compared with the model group, * P<0.05, ** P<0.01, *** P<0.001; compared with the original process group, △ P<0.05, △△△ P<0.001; compared with the control group 1, ※ P<0.05, ※※ P<0.01, ※※※ P<0.001; compared with control group 2, # P<0.05, ## P<0.01, ### P<0.001.

[0210] Based on the above test results, the analgesic and kidney stone treating effects of Example 5 are better than those of the other groups.

[0211] The above embodiments are only intended to help understand the method and core concept of the present invention. It should be noted that, without departing from the principles of the present invention, a number of improvements and modifications may be made to the present invention by those skilled in the art, and such improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A Chinese medicine composition for treating urinary calculi, It is characterized in that In parts by weight, it includes: Extract A 4-12 parts; Extract B 2-6 parts; 10-20 parts of extract C; Extract D 3-7 parts; The extract A is a mixed powder of 3 parts of vinegar Corydalis yanhusuo and 4 parts of boiled chicken gizzard lining; The extract B is a cyclodextrin inclusion compound of 2 parts of steam distilled volatile oil from costusroot; The extract C is a mixture of the residue and distillate of 2 parts of costusroot, 10 parts of stir-fried radix bupleuri, 4 parts of salvia miltiorrhiza, 6 parts of polyanthus macrophylla, 5 parts of superbus, 3 parts of achyranthes bidentata and 4 parts of Lygodium japonicum, which are extracted with water and precipitated with alcohol; The extract D is the ethanol eluate of 10 parts of Lysimachia sibiricum after reflux extraction and passing through a macroporous adsorption resin.

2. The Chinese medicine composition for treating urinary calculi according to claim 1, It is characterized in that include: 4, 8 or 12 parts of extract A; Extract B 2, 4 or 6 parts; Extract C 10, 15 or 20 parts; Extract D 3, 5 or 7 parts.

3. The Chinese medicine composition for treating urinary calculi according to claim 2, It is characterized in that include: Extract A 8 parts; Extract B 4 parts; Extract C 20 parts; Extract D 3 parts.

4. A method for preparing the Chinese medicine composition for treating urinary calculi according to any one of claims 1 to 3, It is characterized in that The following steps are involved: A) 3 parts of vinegar Corydalis yanhusuo and 4 parts of blanched chicken's gizzard lining were ultra-finely ground into fine powder to obtain extract A; B) crushing 2 parts of costusroot into coarse powder, and extracting by steam distillation to obtain volatile oil, medicinal residue and distillate; The volatile oil, β-cyclodextrin and water are mixed to obtain an inclusion compound, which is dried to obtain extract B; C) taking the residue and distillate of the extract B, 10 parts of stir-fried Vaccaria segetalis, 4 parts of Salvia miltiorrhiza, 6 parts of Polygonum multiflorum, 5 parts of Dianthus superbus, 3 parts of Achyranthes bidentata, and 4 parts of Lygodium japonicum, mixing them, and then extracting with water and precipitating with alcohol to obtain extract C; D) taking 10 parts of Lysimachia sibiricum, crushing and then refluxing for extraction, filtering, concentrating the filtrate and passing it through a macroporous adsorption resin, eluting with water, discarding the eluate, eluting with ethanol, collecting the eluate, and concentrating under reduced pressure to obtain an extract to obtain an extract D; E) The extract A, extract B, extract C and extract D are mixed to obtain a Chinese medicine composition for treating urinary system stones.

5. The preparation method according to claim 4, It is characterized in that In the step C), the amount of water extracted is 4 to 10 times the total mass of the drug; After water extraction, the solution is filtered and the filtrate is concentrated to obtain a clear paste; The relative density of the clear paste is 1.10-1.20 (80° C.).

6. The preparation method according to claim 4, It is characterized in that In the step C), the alcohol precipitation is carried out using ethanol; The amount of ethanol used is 60wt% to 80wt% of the clear paste obtained after water extraction; The alcohol precipitation is specifically: Add ethanol to dissolve the clear paste obtained by water extraction, let it stand and then filter it, recover ethanol from the filtrate and concentrate it to a thick paste with a relative density of 1.20-1.30 (80°C).

7. The preparation method according to claim 4, It is characterized in that In the step D), the filtrate is concentrated by: The filtrate is concentrated to 1.0-1.2 g of crude drug per ml.

8. The preparation method according to claim 4, It is characterized in that In the step D), the macroporous adsorption resin is a D101 macroporous adsorption resin; The amount of water used for elution is 2 to 4 times the volume of the column; The amount of ethanol used for elution is 5 to 8 times of column volume; the concentration of ethanol is 50% to 70%.

9. The preparation method according to claim 4, It is characterized in that In the step D), the reduced pressure concentration to obtain an extract is specifically: Concentrate to an extract with a relative density of 1.24-1.26 (80°C).

10. Use of the Chinese medicinal composition for treating urinary calculi according to any one of claims 1 to 3 or the Chinese medicinal composition for treating urinary calculi prepared by the preparation method according to any one of claims 4 to 9 in preparing a drug for treating or alleviating urinary calculi.

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