Method for imitating wild cultivation under cyclobalanopsis glauca of grifola frondosa living body

Abstract

The invention discloses a method for imitating wild cultivation under grifola frondosa living body cyclobalanopsis, and the method comprises the following steps: step 1, selecting a cyclobalanopsis concentrated distribution forest in a mid-subtropical humid monsoon climate region to cultivate grifola frondosa, the altitude range of the forest land is 600-1000 m, the annual average rainfall is 1100-2600 mm, and the annual average rainfall is 1100-2600 mm; the annual average temperature is 6-17 DEG C, and the annual average relative humidity is 80%; 2, a broad-leaved forest with cyclobalanopsis glauca as a dominant tree species should be selected for a cultivation forest land, the canopy density of the forest land is 0.5-0.8, and the gradient is lower than 30 degrees; 3, selecting living cyclobalanopsis glauca as a grifola frondosa hypha inoculation target tree; according to the method for imitating wild cultivation under the cyclobalanopsis glauca of the grifola frondosa living body, a grifola frondosa cultivar culture medium and a preparation process thereof are omitted, light, temperature and humidity management in various growth stages of mycelia and sporocarps is reduced, and the problems that artificial cultivation of grifola frondosa is poor in nutritional value, flavor and taste and high in cultivation cost are solved.

Description

Technical Field

[0001] The invention relates to the technical field of wild-simulated cultivation of Grifola frondosa, in particular to a method for simulating wild-simulated cultivation of living Grifola frondosa under a Cyclobalanopsis glauca tree. Background Art

[0002] Grifola frondosa, commonly known as "maitake mushroom", is a mushroom that can be used for both food and medicine. It often grows wild around chestnut trees and Cyclobalanopsis glauca in summer and autumn. The fruiting body is fleshy, with short stalks and coral-like branches, overlapping into clusters; the smell is rich and refreshing; the meat is crisp and tender, and it will not rot even after long cooking; it has good health care effects and high medicinal value. In recent years, as a health food, it has become popular in markets such as Japan and Singapore. The artificial cultivation technology of Grifola frondosa is mostly a surface soil cultivation mode under the forest. There is a certain gap in the taste and nutritional value of Grifola frondosa compared with wild Grifola frondosa, and wild Grifola frondosa is limited in yield and management technology. Therefore, it is of great value to simulate the wild cultivation of Grifola frondosa under the specific climatic conditions of Fanjing Mountain. To this end, we propose a method for simulating the wild cultivation of Grifola frondosa under living Cyclobalanopsis glauca trees. Summary of the invention

[0003] The technical problem to be solved by the present invention is to overcome the existing defects and provide a method for simulating wild cultivation of living Grifola frondosa under a Cyclobalanopsis glauca tree, which solves the problems of complicated artificial cultivation technology of Grifola frondosa, poor nutritional value and flavor, and high cultivation cost, and can effectively solve the problems in the background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: a method for simulating wild cultivation of living Grifola frondosa under a Cyclobalanopsis glauca tree, the method comprising the following steps:

[0005] Step 1: Select the concentrated distribution forest of Cyclobalanopsis glauca in the mid-subtropical humid monsoon climate area for the cultivation of Grifola frondosa. The forest land has an altitude range of 600-1000 meters and an average annual rainfall of 1100-2600mm. Use a thermometer and hygrometer to conduct real-time detection of the temperature and humidity of the forest land.

[0006] Step 2: For the cultivated forest land, a broad-leaved forest with Cyclobalanopsis as the dominant tree species should be selected, and Cyclobalanopsis microphylla and Cyclobalanopsis glauca are preferred;

[0007] Step 3: Select living Cyclobalanopsis glauca as the target tree for inoculation of Grifola frondosa mycelium. The tree roots should be large and exposed, with natural gullies suitable for the growth of Grifola frondosa, and with a slope without water accumulation. At the same time, ensure that the surrounding water source is clean and pollution-free.

[0008] Step 4: Collect wild maitake mushrooms, wipe and disinfect them with alcohol cotton balls, and then use a sterile knife to cut off uncontaminated tissue blocks at the junction of the cap and stipe of the maitake mushroom, put them in a culture dish, complete tissue separation and inoculation culture on a clean workbench, and provide light and ventilation in time;

[0009] Step 5: After the mycelium culture is completed, use quicklime to disinfect the inoculation area of ​​the living Cyclobalanopsis glauca roots at the end of March, then use a borer to drill 10-15 inoculation holes in the disinfected area, use tweezers to connect the cultured mycelium to the inside of the hole, and plug the hole with columnar sawdust, and disinfect again;

[0010] Step 6: After inoculation, regularly monitor and observe the growth of mycelium and fruiting bodies, and take good care of pests and diseases, rainstorms, and surface runoff.

[0011] Step 7: After the mushrooms of Grifola frondosa are produced from May to September, the best harvesting time is selected according to the growth of the fruiting bodies, and the dead branches and weeds in the harvested Grifola frondosa are cleaned up. After cleaning, the whole mushrooms are dried or stored at a low temperature of 4-8℃.

[0012] Preferably, in step one, in step one, the average annual temperature of the forest land in the mid-subtropical humid monsoon climate region is 6-17°C, and the average annual relative humidity is 80%.

[0013] Preferably, in step 2, the canopy density of the broad-leaved forest is 0.5-0.8, and the slope of the forest is less than 30 degrees.

[0014] Preferably, in step three, the age of the target tree for mycelium inoculation is 60-100 years old.

[0015] Preferably, in step 4, the alcohol concentration of the alcohol cotton ball is 70-75%.

[0016] Preferably, in step 4, the inoculated wild Grifola frondosa culture time is 15-20 days, and the temperature inside the culture dish after inoculation is 20-26°C.

[0017] Preferably, in step five, the specifications of the inoculation hole at the root of the Cyclobalanopsis glauca tree are 1.5-2.0 cm in diameter and 2.0-3.0 cm in depth.

[0018] Preferably, in step five, the mycelium can grow normally within the range of 20-30°C, the optimum temperature range is 24-27°C, the optimum environmental relative humidity is 65%, and the optimum pH range is 5.5-6.5.

[0019] Preferably, in step six, the mycelium can form fruiting bodies at 16-24°C, and the optimum temperature range of the fruiting bodies is 18-21°C, and the optimum relative humidity of the environment is 90%.

[0020] Preferably, in step seven, the harvesting standards of the Grifola frondosa are: ① the cap is an irregular semicircle, extending in an overlapping manner in all directions, the surface is light gray or grayish white, and no grayish white growth ring appears on the edge of the cap, which is slightly curled inward; ② a fungus pore layer appears at a height of 1 cm from the stipe to the ground; ③ there is a strong mushroom aroma.

[0021] Compared with the prior art, the invention has the following beneficial effects: the method for simulating wild cultivation of living maitake mushrooms under Cyclobalanopsis glauca has the following advantages:

[0022] 1. The production efficiency of Grifola frondosa can be improved by limiting the wild Grifola frondosa growth area and forest land, with Fanjing Mountain in Tongren City being the best. At the same time, living Cyclobalanopsis glauca is selected for Grifola frondosa cultivation, which can save the Grifola frondosa cultivation medium and its preparation process, and reduce the light, temperature and humidity management of Grifola frondosa at each growth stage, reduce the cultivation cost, and solve the problem of poor nutritional value and flavor and taste of artificially cultivated Grifola frondosa.

[0023] 2. Provide sufficient light and ventilation during the mycelium culture process and strictly control the temperature inside the culture dish to prevent the mycelium from lacking oxygen, excessive light and other problems inside the culture dish, thereby avoiding the occurrence of yellow water problems;

[0024] 3. Monitor the growth of mycelium and fruiting bodies through multiple inspections, prevent and control diseases and pests of Grifola frondosa in a timely manner, and dig drainage ditches before the arrival of the rapid rain season to prevent heavy rain and surface runoff from causing losses to the yield of Grifola frondosa fruiting bodies.

[0025] 4. Clean the dead branches and weeds in the harvested maitake mushrooms to prevent impurities from affecting the quality of the maitake mushrooms. At the same time, dry the cleaned maitake mushrooms or store them at a low temperature of 4-8℃ to facilitate subsequent storage and transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 : It is a schematic diagram of the cultivation method of the present invention; DETAILED DESCRIPTION

[0027] The following will be combined with the appended Figure 1 , the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Embodiment 1:

[0029] A method for simulating wild cultivation of living Grifola frondosa under a Cyclobalanopsis glauca tree, the method comprising the following steps:

[0030] Step 1: Select the concentrated distribution forest of Cyclobalanopsis glauca in the mid-subtropical humid monsoon climate area to cultivate Grifola frondosa. The forest land has an altitude range of 600-1000 meters, an average annual rainfall of 1100-2600 mm, an average annual temperature of 6-17°C, and an average annual relative humidity of 80%. The locations of the Grifola frondosa cultivation demonstration points are as follows: Demonstration point ①: 109°22'41.3879", 27°40'40.6432", 600m above sea level; Demonstration point ②: 108°47'45.4387", 27°53'06.9665", 831m above sea level; Demonstration point ③: 108°47'45.4387", 27°53'06.9665", 1000m above sea level;

[0031] Step 2: For the cultivated forest land, a broad-leaved forest with Cyclobalanopsis as the dominant tree species should be selected, especially Cyclobalanopsis microphylla and Cyclobalanopsis glauca. The forest canopy density should be 0.5-0.8 and the slope should be less than 30 degrees.

[0032] Step 3: Select living Cyclobalanopsis glauca as the target tree for inoculation of Grifola frondosa mycelium. The tree roots of the cultivated tree should be large and exposed, with natural gullies suitable for the growth of Grifola frondosa, and with a slope without water accumulation. At the same time, ensure that the surrounding water source is clean and pollution-free. The cultivated tree age is 60-100 years old;

[0033] Step 4: Collect wild maitake mushrooms, wipe and disinfect them with alcohol cotton balls, the alcohol concentration of the alcohol cotton balls is 70-75%, use a sterile knife to cut off uncontaminated tissue blocks at the junction of the cap and stipe of the maitake mushrooms, put them in a culture dish, and complete tissue separation and inoculation culture on an ultra-clean workbench. Use a light to supplement the light according to the growth of the hyphae. The wild maitake mushrooms in the culture dish after inoculation take 15-20 days to be cultured, and the temperature inside the culture dish after inoculation is 20-26°C;

[0034] Step 5: After the mycelium culture is completed, use quicklime to disinfect the inoculation area of ​​the living Cyclobalanopsis glauca roots at the end of March, and then use a borer to drill 10-15 inoculation holes in the disinfected area. The inoculation hole specifications are 1.5-2.0 cm in diameter and 2.0-3.0 cm in depth. Use tweezers to connect the cultured mycelium to the inside of the hole, and block the hole mouth with columnar sawdust, and disinfect again; the mycelium can grow normally in the range of 20-30℃, the optimum temperature is 24-27℃, the optimum relative humidity is 65%, and the optimum pH value is 5.5-6.5;

[0035] Step 6: After the inoculation is completed, regularly monitor and observe the growth of mycelium and fruiting bodies, and do a good job in preventing and controlling pests and diseases, rainstorms and surface runoff. Grifola frondosa mycelium can form fruiting bodies at 16-24°C, the optimum temperature for fruiting bodies is 18-21°C, and the optimum relative humidity is 90%;

[0036] Step 7: After the mushrooms of Grifola frondosa are produced from May to September, the best harvesting time is selected according to the growth of the fruiting bodies, and the dead branches and weeds in the harvested Grifola frondosa are cleaned up. After cleaning, the whole mushroom is dried or stored at a low temperature of 4-8℃. The harvesting standards of Grifola frondosa are: ① The cap is an irregular semicircle, extending in an overlapping manner to the surroundings, and the surface is light gray or grayish white. There is no grayish white growth ring at the edge of the cap, which is slightly curled inward; ② A fungus pore layer appears at a height of 1 cm from the ground to the stem; ③ There is a strong mushroom fragrance.

[0037] Demonstration sites for Grifola frondosa were established at different altitudes, and the temperature, rainfall, and other environmental indicators of each demonstration site were measured. After two consecutive years of follow-up investigation, the following is a display of the mushroom production of Grifola frondosa at different altitudes:

[0038] Table 1 The production of Grifola frondosa mushrooms at different altitudes

[0039]

[0040] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca, characterized in that: The method for simulating wild cultivation of living maitake mushrooms under Cyclobalanopsis glauca comprises the following steps: Step 1: Select the concentrated distribution forest of Cyclobalanopsis glauca in the mid-subtropical humid monsoon climate area for the cultivation of Grifola frondosa. The forest land has an altitude range of 600-1000 meters and an average annual rainfall of 1100-2600mm. Use a thermometer and hygrometer to conduct real-time detection of the temperature and humidity of the forest land. Step 2: For the cultivated forest land, a broad-leaved forest with Cyclobalanopsis as the dominant tree species should be selected, and Cyclobalanopsis microphylla and Cyclobalanopsis glauca are preferred; Step 3: Select living Cyclobalanopsis glauca as the target tree for inoculation of Grifola frondosa mycelium. The tree roots should be large and exposed, with natural gullies suitable for the growth of Grifola frondosa, and with a slope without water accumulation. At the same time, ensure that the surrounding water source is clean and pollution-free. Step 4: Collect wild maitake mushrooms, wipe and disinfect them with alcohol cotton balls, and then use a sterile knife to cut off uncontaminated tissue blocks at the junction of the cap and stipe of the maitake mushroom, put them in a culture dish, complete tissue separation and inoculation culture on a clean workbench, and provide light and ventilation in time; Step 5: After the mycelium culture is completed, use quicklime to disinfect the inoculation area of ​​the living Cyclobalanopsis glauca roots at the end of March, then use a borer to drill 10-15 inoculation holes in the disinfected area, use tweezers to connect the cultured mycelium to the inside of the hole, and plug the hole with columnar sawdust, and disinfect again; Step 6: After inoculation, regularly monitor and observe the growth of mycelium and fruiting bodies, and take good care of pests and diseases, rainstorms, and surface runoff. Step 7: After the mushrooms of Grifola frondosa are produced from May to September, the best harvesting time is selected according to the growth of the fruiting bodies, and the dead branches and weeds in the harvested Grifola frondosa are cleaned up. After cleaning, the whole mushrooms are dried or stored at a low temperature of 4-8℃.

2. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step 1, the average annual temperature of the forest land in the mid-subtropical humid monsoon climate region is 6-17°C, and the average annual relative humidity is 80%.

3. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step 2, the canopy density of the broad-leaved forest is 0.5-0.8, and the slope of the forest is less than 30 degrees.

4. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step three, the age of the target tree for mycelium inoculation is 60-100 years old.

5. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step 4, the alcohol concentration of the alcohol cotton ball is 70-75%.

6. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step 4, the inoculated wild Grifola frondosa culture time is 15-20 days, and the temperature inside the culture dish after inoculation is 20-26°C.

7. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step five, the inoculation hole at the root of the Cyclobalanopsis glauca has a diameter of 1.5-2.0 cm and a depth of 2.0-3.0 cm.

8. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step 5, the mycelium can grow normally within the range of 20-30°C, the optimum temperature range is 24-27°C, the optimum environmental relative humidity is 65%, and the optimum pH range is 5.5-6.

5.

9. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step six, the mycelium can form fruiting bodies at 16-24° C., and the optimum temperature range of the fruiting bodies is 18-21° C., and the optimum relative humidity of the environment is 90%.

10. The method for simulating wild cultivation of living Grifola frondosa under Cyclobalanopsis glauca according to claim 1, characterized in that: In step seven, the harvesting standards of the Grifola frondosa are as follows: ① the cap is an irregular semicircle, extending in an overlapping manner, the surface is light gray or grayish white, and no grayish white growth ring appears on the edge of the cap, which is slightly curled inward; ② a fungus pore layer appears at a height of 1 cm from the stipe to the ground; ③ there is a strong mushroom aroma.

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