Radix astragali and rhizoma polygonati oral liquid and preparation method thereof

Abstract

The invention belongs to the field of traditional Chinese medicine, and particularly relates to astragalus membranaceus and rhizoma polygonati oral liquid and a preparation method thereof. The invention provides a radix astragali and rhizoma polygonati oral liquid. The radix astragali and rhizoma polygonati oral liquid comprises the following components in parts by weight: 25-35 parts of radix astragali, 25-35 parts of rhizoma polygonati, 25-35 parts of radix asteris and 10-25 parts of radix codonopsis which are used as monarch drugs; 10-20 parts of bighead atractylodes rhizome, 25-35 parts of poria cocos, 25-35 parts of prepared rehmannia root and 15-20 parts of caffeoyl butanediamine which are used as ministerial drugs; 10-20 parts of angelica sinensis, 25-35 parts of fructus lycii and 10-20 parts of herba ecliptae are used as adjuvant drugs; and 5-10 parts of honey-fried licorice root and 10-20 parts of glossy privet fruit as conductant drugs. According to the monarch, minister, assistant and guide medication idea of traditional Chinese medicine, caffeoyl butanediamine is matched, so that the oral liquid can effectively tonify qi and enrich blood, exert the curative effect of tonifying qi and boost the spirit of a patient, and has good social benefits.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and specifically relates to an astragalus and polygonatum oral liquid and a preparation method thereof. Background Art

[0002] Astragalus is a traditional Chinese herbal medicine that contains a variety of effective ingredients, such as astragalus polysaccharides. Astragalus polysaccharides have immunomodulatory effects, which can enhance the body's immunity and improve the body's resistance to pathogens. For example, it can stimulate the activity of immune cells such as macrophages, allowing these cells to better engulf foreign pathogens. Astragalus also contains flavonoids, which have antioxidant effects and can remove excess free radicals in the body and reduce the damage to cells by free radicals, thereby helping to delay cell aging.

[0003] The main components of Polygonatum sibiricum include Polygonatum sibiricum polysaccharide, steroidal saponin, etc. Polygonatum sibiricum polysaccharide can regulate blood sugar and stabilize blood sugar levels by promoting insulin secretion or increasing insulin sensitivity. It may have a certain auxiliary improvement effect on some patients with prediabetes or mild diabetes.

[0004] With the acceleration of the pace of modern life and the increase of life pressure, people's physical sub-health status is becoming more and more common. Problems such as insufficient qi and blood, low immunity, etc. seriously affect people's quality of life. Traditional Chinese medicine has a long history and rich experience in conditioning the body and strengthening the physical fitness. Astragalus and Polygonatum sibiricum, as traditional Chinese medicinal materials, have significant effects in replenishing qi and blood, enhancing the body's immunity, etc., but the relevant preparations on the market are still insufficient in formula optimization, preparation process and application expansion. As a result, the effect of Astragalus and Polygonatum sibiricum when used together is not good, and their qi-replenishing effect cannot be exerted. Summary of the invention

[0005] The present invention aims to solve the technical problem that the combined use of Astragalus and Polygonatum odoratum in the above-mentioned background technology has poor effect and cannot exert its Qi-invigorating effect. The technical solution adopted by the present invention is as follows: On the one hand, the present invention provides an Astragalus and Polygonatum oral liquid, which comprises the following ingredients by weight: As the main medicine, 25-35 parts of Astragalus, 25-35 parts of Polygonatum, 25-35 parts of Aster, and 10-25 parts of Codonopsis; As adjuvant drugs, 10-20 parts of Atractylodes macrocephala, 25-35 parts of Poria cocos, 25-35 parts of Rehmannia glutinosa, and 15-20 parts of caffeic acid butanediamine; As adjuvants, 10-20 parts of Chinese Angelica, 25-35 parts of Lycium barbarum, and 10-20 parts of Eclipta prostrata; As adjuvant drugs, 5-10 parts of roasted licorice root and 10-20 parts of Ligustrum lucidum fruit are used.

[0006] The beneficial effects of the present invention are as follows: the Astragalus and Polygonatum oral liquid of the present invention, in accordance with the TCM medication concept of monarch, minister, assistant and envoy, is combined with caffeine, can effectively invigorate qi and blood, exert its qi-invigorating effect, boost the spirit of the patient, and has good social benefits.

[0007] Based on the above technical solution, the present invention can also be improved as follows.

[0008] Furthermore, the Astragalus and Polygonatum oral liquid also includes a preservative and a pH regulator.

[0009] The beneficial effect of adopting the above further scheme is that the preservative can prolong the use time of the Astragalus and Polygonatum oral liquid, and the pH regulator can better improve the efficacy of the medicine.

[0010] Furthermore, the preservative is selected from at least one of sodium benzoate and potassium sorbate.

[0011] The beneficial effect of adopting the above further scheme is that the above preservatives can extend the shelf life of the drug while reducing the impact on the efficacy of the drug itself.

[0012] Furthermore, the pH adjuster is selected from at least one of citric acid and sodium dihydrogen phosphate.

[0013] The beneficial effect of adopting the above further scheme is that the above pH adjuster can improve the effect while reducing the impact on the drug.

[0014] Furthermore, the pH value of the Astragalus and Polygonatum oral liquid is 4.5-6.5.

[0015] The beneficial effect of adopting the above further scheme is that the above pH range can better improve the drug efficacy.

[0016] Furthermore, the pH value of the Astragalus and Polygonatum oral liquid is 6.

[0017] The beneficial effect of adopting the above further scheme is that the drug effect is best at the above pH value.

[0018] Furthermore, in parts by weight, the formula includes 29 parts of astragalus, 30 parts of polygonatum, 27 parts of aster, 20 parts of codonopsis, 15 parts of atractylodes, 26 parts of poria, 20 parts of rehmannia root, 15 parts of angelica, 25 parts of wolfberry, 15 parts of eclipta prostrata, 10 parts of licorice, 15 parts of glossy privet, and 18 parts of caffeine.

[0019] The beneficial effect of adopting the above further scheme is that the above determined formula can enhance the effect, make the main drug, the minister drug, the adjuvant drug and the guiding drug cooperate better, and best exert their efficacy.

[0020] In order to realize the above dosage forms, pharmaceutically acceptable excipients need to be added when preparing these dosage forms, such as fillers, disintegrants, lubricants, suspending agents, binders, sweeteners, flavoring agents, bases, etc. Fillers include: starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose, etc.; disintegrants include: starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone, low-substituted hydroxypropyl cellulose, cross-linked sodium carboxymethyl cellulose, etc.; lubricants include: magnesium stearate, sodium lauryl sulfate, talc, silicon dioxide, etc.; suspending agents include: polyvinyl pyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methylcellulose, etc.; binders include: starch slurry, polyvinyl pyrrolidone, hydroxypropyl methylcellulose, etc.; sweeteners include: saccharin sodium, aspartame, sucrose, sodium cyclamate, glycyrrhetinic acid, etc.; flavoring agents include: sweeteners and various flavors; matrices include: PEG6000, PEG4000, insect wax, etc. In order to make the above dosage forms able to realize Chinese medicine pharmacy, it is necessary to add other pharmaceutically acceptable excipients when preparing these dosage forms (the excipients recorded in each dosage form in Fan Biting's "Chinese Medicine Pharmacy", Shanghai Science Press, 1st edition, December 1997).

[0021] Another aspect of the present invention provides a method for preparing the Astragalus and Polygonatum oral liquid, comprising the following steps: Step S1, washing and processing Astragalus, Polygonatum, Aster, Codonopsis, Atractylodes, Poria, Rehmannia glutinosa, Angelica, Lycium barbarum, Ligustrum lucidum, Eclipta prostrata, and Glycyrrhiza uralensis respectively, extracting effective ingredients by water extraction, alcohol extraction, or water-alcohol combined extraction, and concentrating to prepare an extract; Step S2, weighing the extract according to the component ratio of Huangqi Huangjing oral liquid, adding purified water and stirring to fully dissolve the components, then adding caffeic acid diamine and a preservative and stirring to obtain a solution, and adjusting the pH value of the solution to 4.5-6.5 with a pH adjuster; Step S3, filtering the solution in step S2 with a 0.22-0.45 μm microporous filter membrane for sterilization, and quantitatively filling the filtrate into an oral liquid bottle and sealing it.

[0022] The beneficial effect of the present invention is that the Astragalus and Polygonatum oral liquid which can effectively invigorate qi and nourish blood and exert its qi-invigorating effect can be smoothly prepared through the above steps.

[0023] Based on the above technical solution, the present invention can also be improved as follows.

[0024] Furthermore, in step S1, an ethanol extraction method is adopted.

[0025] The beneficial effect of the present invention is that an extract with better quality can be obtained.

[0026] Another aspect of the present invention also provides the use of the Astragalus and Polygonatum oral liquid in the preparation of qi-invigorating and blood-tonifying medicines.

[0027] The beneficial effect of the present invention is that it can provide the Huangqi and Huangjing oral liquid which is effective in invigorating qi and nourishing blood. DETAILED DESCRIPTION

[0028] The principles and features of the present invention are described below. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0029] Definition of terms: According to the above contents of the present disclosure, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present disclosure, other various forms of modifications, replacements or changes may be made.

[0030] The formula of the Huangqi Huangjing oral liquid of the present invention follows the principle of monarch, minister, assistant and envoy. The principle of monarch, minister, assistant and envoy of Chinese medicine is the basic principle of compatibility of Chinese medicine prescriptions, which highly summarizes the different positions and functions of Chinese medicine in prescriptions. The following is a detailed introduction: Junyao Definition: It is a medicine that plays a major therapeutic role on the main disease or main symptom and is an indispensable core part of the prescription.

[0031] Function: The main drug is the most powerful in the prescription, and its dosage is generally large. It plays a leading role in the prescription and can lead the whole prescription to play a major therapeutic role for the main symptoms. For example, in Mahuang Decoction, Mahuang induces sweating and dispels the wind and cold evil, and is the main drug. It can target the main cause of the external wind-cold syndrome - the wind-cold evil binding the surface, and play a major therapeutic role. It is the key drug for the whole prescription to play its role.

[0032] Minister medicine Definition: There are two meanings. One is that it is a drug that assists the main drug to strengthen the treatment of the main disease or main symptom; the other is that it is a drug that plays a therapeutic role for the concurrent disease or concurrent symptom.

[0033] Function: The efficacy of the assistant drug is less than that of the monarch drug. As an assistant drug that assists the monarch drug in strengthening the treatment of the main disease or main symptom, it can enhance the efficacy of the monarch drug. For example, in Mahuang Decoction, Guizhi is the assistant drug, which helps Mahuang to sweat and relieve the exterior symptoms. The two are used together to enhance the power of sweating and dispelling cold, and more effectively relieve the main symptoms of wind-cold exterior syndrome. Assistant drugs that play a therapeutic role for concurrent diseases or concurrent syndromes can take into account other symptoms of the patient in addition to the main disease. For example, in Jianpi Pills for the treatment of spleen deficiency and food accumulation, Baizhu and Muxiang are assistant drugs. Baizhu replenishes qi and strengthens the spleen, while Muxiang promotes qi and removes stagnation. On the basis of the monarch drugs such as ginseng, Baizhu, and tangerine peel that strengthen the spleen and replenish qi, it enhances the function of strengthening the spleen and digesting food, and plays a therapeutic role for the concurrent syndrome of food accumulation and qi stagnation.

[0034] Adjuvant Definition: There are three meanings. The first is adjuvant drugs, which are drugs that assist the main and auxiliary drugs to strengthen the therapeutic effect, or directly treat secondary symptoms; the second is auxiliary drugs, which are drugs used to eliminate or reduce the toxicity or potency of the main and auxiliary drugs; the third is counter-adjuvant drugs, which are drugs that have opposite properties to the main drugs but can play a complementary role in treatment according to the needs of the disease.

[0035] Function: Auxiliary drugs can enhance the therapeutic effect of the main and auxiliary drugs. For example, in Yinqiao Powder, adjuvant drugs such as Platycodon grandiflorum and Burdock Fructus can assist the main drugs of Honeysuckle and Forsythiae in clearing away heat and detoxification, dispersing wind and heat, making the whole prescription more powerful in dispersing wind and heat, and better treating wind-heat colds. Auxiliary drugs can restrain the harshness or toxicity of the main and auxiliary drugs. For example, in Sini Decoction, Zhigancao is an adjuvant drug, which can alleviate the harshness of dried ginger and aconite, so that it can break the yin and return the yang without the risk of violent dispersion, and reduce the toxic side effects of the drugs. Counter-adjuvant drugs are used when the disease is serious and the evil is likely to refuse the drug, so as to prevent the pathogenic evil from rejecting the drug. For example, in Baitong plus pig bile soup, pig bile and human urine are counter-adjuvant drugs, which are salty and cold in nature. Adding a small amount of cold pig bile and human urine to a large amount of warm ginger, aconite and other drugs can prevent the excessive yin and cold from rejecting the hot drugs, so that it can better play the role of returning yang and rescuing the adverse.

[0036] Drug Definition: There are two meanings. One is the guiding drug, which is the drug that can lead the other drugs in the prescription directly to the site of the disease; the other is the harmonizing drug, which is the drug that has the function of harmonizing other drugs.

[0037] Function: Channel-guiding drugs can guide the power of other drugs in the prescription to the lesion site, improving the pertinence and efficacy of the drugs. For example, in Qianghuo Shengshi Decoction, Qianghuo and Duhuo are the main drugs, Fangfeng, Ligusticum chuanxiong, etc. are the ministerial drugs, Chuanxiong is the adjuvant, and Asarum is the messenger drug. Its nature is good at running around, which can lead the drugs to go up to the head and face, directly to the diseased area, better play the role of expelling wind, eliminating dampness and relieving pain, and treat rheumatism in the surface headache, body pain and other symptoms. Harmonizing drugs can make the drugs in the prescription coordinate with each other, play a therapeutic role together, and make the drug properties of the whole prescription tend to be peaceful. For example, in Guizhi Decoction, Zhigancao can not only reconcile the pungent, dispersing, sour and astringent nature of Guizhi and Shaoyao, but also reconcile the other drugs, making the whole prescription rigorous, with astringent in the dispersion and tonic in the sweat, becoming a classic prescription for harmonizing Ying and Wei.

[0038] The term "treat" as used herein means to alleviate or alleviate at least one symptom of a disease in a subject. Within the meaning of the present disclosure, the term "treat" also refers to inhibiting, delaying the onset (i.e., the pre-clinical manifestation of the disease) and / or reducing the risk of developing or worsening the disease.

[0039] The term “comprising” encompasses the meanings of “including” and “consisting of”, for example, a composition “comprising” X may consist of X alone, or may include some other components, such as X+Y.

[0040] The Huangqi Huangjing oral solution of the present disclosure can be administered orally, topically, parenterally or mucosally (e.g., buccally, by inhalation or rectally) in a dosage unit formulation containing a conventional non-toxic pharmaceutically acceptable carrier. It is usually desirable to use the oral route.

[0041] The Huangqi Huangjing oral liquid of the present invention can be prepared according to known methods, such as the methods described in the general rules for preparation of the 16th edition of the Japanese Pharmacopoeia, the United States Pharmacopoeia, and the 9th edition of the European Pharmacopoeia. The specific preparation method depends on the dosage form.

[0042] The "effective amount" described in the present invention refers to an amount sufficient to induce a desired biological response. The effective amount of the components of the Huangqi Huangjing oral solution of the present invention may vary depending on factors such as the desired biological endpoint, the pharmacokinetics of the compound, the condition being treated, the mode of administration, and the age and health of the subject. In certain embodiments, the effective amount is a therapeutically effective amount. The effective amount is the amount of the first component described in the present invention in a single dose.

[0043] Example 1, preparation of Astragalus and Polygonatum oral liquid

[0044] Step S1, washing and processing Astragalus, Polygonatum, Aster, Codonopsis, Atractylodes, Poria, Rehmannia glutinosa, Angelica, Lycium barbarum, Ligustrum lucidum, Eclipta prostrata, and Glycyrrhiza uralensis respectively, extracting effective ingredients by water extraction, alcohol extraction, or water-alcohol combined extraction, and concentrating to prepare an extract, as follows: Astragalus processing: Wash and dry the Astragalus herb and crush it into coarse powder. Use water extraction and alcohol precipitation method to extract, soak the Astragalus coarse powder with 10 times the amount of water for 2 hours, then heat and reflux to extract twice, each time for 1 hour, combine the extracts, filter, and decompress the filtrate to a relative density of 1.05 (60℃), add 95% ethanol to make the alcohol content reach 70%, let it stand for 48 hours, take the supernatant, recover the ethanol, and concentrate it to a thick paste.

[0045] Processing of Polygonatum sibiricum: Wash, steam and slice the Polygonatum sibiricum, extract with 6 times the amount of 70% ethanol under reflux twice, 2 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.05 (60°C), recover the ethanol, and concentrate to a thick paste.

[0046] Aster treatment: Grind into coarse powder, extract with 9 times the amount of 60% ethanol under reflux for 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60°C).

[0047] Processing of Codonopsis pilosula: wash, cut into sections, soak in 6 times the amount of water for 2 hours, heat and reflux to extract twice, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate to an extract with a relative density of 1.05 (60℃).

[0048] Atractylodes macrocephala processing: wash, slice, extract with 9 times the amount of 60% ethanol reflux for 3 times, 1.5 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60°C).

[0049] Poria processing: Grind into coarse powder, boil and extract with 10 times the amount of water for 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate to an extract with a relative density of 1.05 (60℃).

[0050] Processing of Rehmannia glutinosa: cut into small pieces, soak in 8 times the amount of 70% ethanol for 3 hours, reflux extraction 3 times, 1.5 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60℃).

[0051] Angelica treatment: Wash, slice, and extract with 9 times the amount of 60% ethanol reflux for 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60°C).

[0052] Treatment of wolfberry: Wash the wolfberry, soak it in 5 times the amount of 60% ethanol for 3 hours, reflux extraction 3 times, 1.5 hours each time, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60℃).

[0053] Treatment of Ligustrum lucidum fruit: wash, extract with 8 times the amount of 70% ethanol reflux for 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60℃).

[0054] Treatment of Eclipta prostrata: Wash, cut into sections, and extract with 9 times the amount of 60% ethanol reflux for 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to an extract with a relative density of 1.05 (60℃).

[0055] Treatment of roasted licorice: Wash the roasted licorice, crush it into coarse powder, soak it in 10 times the amount of water for 2 hours, heat and reflux to extract 3 times, each time for 1.5 hours, combine the extracts, filter, and concentrate the filtrate to an extract with a relative density of 1.05 (60℃).

[0056] Step S2, weigh the above extracts according to 35g of Astragalus, 35g of Polygonatum, 35g of Aster, 25g of Codonopsis, 20g of Atractylodes, 35g of Poria, 35g of Rehmannia glutinosa, 20g of Angelica sinensis, 35g of Lycium barbarum, 20g of Ligustrum lucidum, 20g of Eclipta prostrata, and 10g of Radix Glycyrrhizae Preparata, add purified water and stir to fully dissolve the components, then add 20g of caffeic acid butanediamide, then add sodium benzoate as a preservative and stir evenly, and adjust the pH value of the solution to 6 with citric acid as a pH adjuster; Step S3, filtering the above solution with a 0.22 μm microporous filter membrane for sterilization, and quantitatively filling the filtrate into an oral liquid bottle and sealing it.

[0057] In the above preparation method, the raw material drug can be purified and refined after extraction, such as passing through a macroporous resin column; and further prepared into any conventional dosage form acceptable to pharmacy according to conventional preparation technology, including granules, tablets, capsules, pills, oral liquids, suspensions, emulsions, and injections.

[0058] In order to realize the above dosage forms, pharmaceutically acceptable excipients need to be added when preparing these dosage forms, such as fillers, disintegrants, lubricants, suspending agents, binders, sweeteners, flavoring agents, bases, etc. Fillers include: starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose, etc.; disintegrants include: starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone, low-substituted hydroxypropyl cellulose, cross-linked sodium carboxymethyl cellulose, etc.; lubricants include: magnesium stearate, sodium lauryl sulfate, talc, silicon dioxide, etc.; suspending agents include: polyvinyl pyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methylcellulose, etc.; binders include: starch slurry, polyvinyl pyrrolidone, hydroxypropyl methylcellulose, etc.; sweeteners include: saccharin sodium, aspartame, sucrose, sodium cyclamate, glycyrrhetinic acid, etc.; flavoring agents include: sweeteners and various flavors; matrices include: PEG6000, PEG4000, insect wax, etc. In order to make the above dosage forms able to realize Chinese medicine pharmacy, it is necessary to add other pharmaceutically acceptable excipients when preparing these dosage forms (the excipients recorded in each dosage form in Fan Biting's "Chinese Medicine Pharmacy", Shanghai Science Press, 1st edition, December 1997).

[0059] Example 2, Preparation of Astragalus and Polygonatum oral liquid The difference from Example 1 is that in step S2, 29 g of Astragalus, 30 g of Polygonatum, 27 g of Aster, 20 g of Codonopsis, 15 g of Atractylodes, 26 g of Poria, 20 g of Rehmannia glutinosa, 15 g of Angelica sinensis, 25 g of Lycium barbarum, 15 g of Ecliptae, 10 g of Radix Glycyrrhizae Preparata, and 15 g of Ligustrum lucidum are weighed, and 18 g of caffeoyl butanediamide is added.

[0060] Example 3, Preparation of Astragalus and Polygonatum oral liquid The difference from Example 1 is that in step S2, 25g of Astragalus, 25g of Polygonatum, 25g of Aster, 10g of Codonopsis, 10g of Atractylodes, 25g of Poria, 25g of Rehmannia glutinosa, 10g of Angelica sinensis, 25g of Lycium barbarum, 10g of Eclipta prostrata, 5g of Radix Glycyrrhizae Preparata, and 10g of Ligustrum lucidum are weighed as the above extracts, and 15g of caffeic acid butanediamide is added.

[0061] Example 4: Experimental study on the therapeutic effect of Huangqi Huangjing oral liquid on rat models

[0062] Experimental animals: 48 clean Wistar rats, half male and half female, weighing (180±20) g.

[0063] method: 1. Grouping and administration: divided into negative control group, blank control group, experimental group 1 (taking the Huangqi Huangjing oral liquid of Example 1), experimental group 2 (taking the Huangqi Huangjing oral liquid of Example 2), experimental group 3 (taking the Huangqi Huangjing oral liquid of Example 3), positive control group (commercially purchased donkey-hide gelatin blood-replenishing solution 2 mg kg -1 ).in: The negative control group was not given drugs by gavage and was kept normally; The positive control group was given 2 mg·kg of donkey-hide gelatin blood-enriching solution by gavage. -1 , once a day, for 12 consecutive days.

[0064] The blank control group was given normal saline, 1 mL per 100 g body weight, once a day for 12 consecutive days; The experimental group was given the corresponding drug solution by gavage, with a volume of 1 mL per 100 g body weight, once a day for 12 consecutive days; 2. Modeling Except for the negative control group, all groups were subcutaneously injected with 2% N-acetylphenylhydrazine saline solution on days 1, 3, 5, 7, and 9, with doses of 100, 50, 50, 25, and 25 mg kg -1 , resulting in a blood deficiency model.

[0065] 3. Index detection (1) Changes in rats’ physical signs, rectal temperature and facial temperature; (2) Bleeding and coagulation time: Cut the rat's tail 3 cm from the tip, discard the first drop of blood, and drop the blood on both ends of a clean glass slide. The diameter of the blood drop is 5-10 mm. Start timing immediately. Use a dry needle to stir the blood once every 30 seconds until the needle can stir up the fibrin thread. This is the coagulation time. After the rat's tail is cut, wait for the blood to overflow by itself and start timing. Use filter paper to absorb the blood drop once every 30 seconds until the bleeding stops naturally (no blood is absorbed by the filter paper). This is the bleeding time. (3) Red blood cell count (RBC) and hematocrit (HCT) testing: blood is drawn from the ocular plexus vein to measure hematological indicators; 4. Statistical methods The data were expressed as x±s, and the comparison among the groups was performed by analysis of variance using the statistical software SPSS 11.0.

[0066] 5. Results 5.1. Impact on facial temperature and rectal temperature Compared with the negative control group, the facial and rectal temperatures of rats in the blank control group decreased significantly ( P <0.01); compared with the positive control group, experimental groups 1, 2 and 3 could significantly increase facial temperature and rectal temperature ( P <0.05), indicating that the experimental group had an improvement in facial temperature and rectal temperature. The results are shown in Table 1.

[0067] Table 1

[0068] Note: Compared with the negative control group, 1) P <0.01; compared with the blank control group, 2) P <0.05 5.2 Effects on Bleeding and Coagulation Time Compared with the negative control group, the coagulation time and bleeding time of the rats in the blank control group were significantly prolonged (P<0.01); compared with the positive control group, experimental groups 1, 2 and 3 could significantly shorten the coagulation time and bleeding time of the rats (P<0.05), among which experimental group 2 had the best effect. The results are shown in Table 2.

[0069] Table 2

[0070] Note: Compared with the negative control group, 1) P<0.01; compared with the blank control group, 2) P<0.05, 3) P<0.01 5.3. Red blood cell count (RBC) and hematocrit (HCT) testing Compared with the negative control group, the RBC and HCT of the rats in the blank control group were significantly decreased ( P <0.01); compared with the positive control group, it can significantly increase RBC and HCT ( P <0.01), experimental groups 1, 2 and 3 can increase RBC, HCT ( P <0.05), and elevated RBC ( P <0.05). This indicates that experimental groups 1, 2 and 3 can promote hematopoiesis, and experimental group 2 has the best promoting effect. See Table 3 for details: Table 3

[0071] Note: Compared with the negative control group, 1) P <0.01; compared with the positive control group, 2) P <0.05, 3) P <0.01

[0072] Example 5: Anti-fatigue application test 1. Methods Forty athletes were selected and randomly divided into two groups, with 20 people in each group.

[0073] The volunteers in the experimental group took Astragalus and Polygonatum oral liquid once in the morning and evening every day, with each dose being 20 ml, for 4 weeks.

[0074] The volunteers in the control group ate a normal diet every morning and evening for 4 weeks.

[0075] 2. Sports endurance test Before and after the experiment, all volunteers underwent an endurance running test on a standard 400-meter track. The volunteers were required to run at a constant speed until they were exhausted and could no longer maintain the running posture. The total running time was recorded.

[0076] 3. Fatigue recovery time test Before and after the experiment, after completing the endurance running test to exhaustion, the volunteers rested in a quiet and comfortable environment, and recorded the time from stopping running to the heart rate returning to the pre-exercise range of +10 beats / minute as the fatigue recovery time. The total running time and fatigue recovery time were calculated, and the results are shown in Table 4. Table 4

[0077] The sports endurance of the athletes in the experimental group was significantly improved compared with that in the control group. At the same time, the fatigue recovery time of the athletes in the experimental group after training was shortened by about 30% compared with that in the control group.

[0078] In the description of this specification, the description of the terms "one embodiment", "some embodiments", "specific embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0079] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An Astragalus and Polygonatum oral liquid, characterized in that: The invention comprises the following ingredients by weight: 25-35 parts of astragalus as main medicine, 25-35 parts of polygonatum, 25-35 parts of aster, 10-25 parts of codonopsis; As adjuvant drugs, 10-20 parts of Atractylodes macrocephala, 25-35 parts of Poria cocos, 25-35 parts of Rehmannia glutinosa, and 15-20 parts of caffeic acid butanediamine; As adjuvants, 10-20 parts of Chinese Angelica, 25-35 parts of Lycium barbarum, and 10-20 parts of Eclipta prostrata; As adjuvant drugs, 5-10 parts of roasted licorice root and 10-20 parts of Ligustrum lucidum fruit are used.

2. The Astragalus and Polygonatum oral liquid according to claim 1, characterized in that The Huangqi Huangjing oral liquid also includes a preservative and a pH regulator.

3. The Astragalus and Polygonatum oral liquid according to claim 2, characterized in that: The preservative is selected from at least one of sodium benzoate and potassium sorbate.

4. The Astragalus and Polygonatum oral liquid according to claim 2, characterized in that The pH regulator is selected from at least one of citric acid and sodium dihydrogen phosphate.

5. The Astragalus and Polygonatum oral liquid according to claim 4, characterized in that: The pH value of the Astragalus and Polygonatum oral liquid is 4.5-6.

5.

6. The Astragalus and Polygonatum oral liquid according to claim 5, characterized in that: The pH value of the Astragalus and Polygonatum oral liquid is 6.

7. The Astragalus and Polygonatum oral liquid according to any one of claims 1 to 6, characterized in that: In parts by weight, the formula includes 29 parts of astragalus, 30 parts of polygonatum, 27 parts of aster, 20 parts of codonopsis, 15 parts of atractylodes, 26 parts of poria, 20 parts of rehmannia root, 15 parts of angelica, 25 parts of wolfberry, 15 parts of eclipta prostrata, 10 parts of licorice, 15 parts of ligustrum lucidum, and 18 parts of caffeamide.

8. The method for preparing the Astragalus and Polygonatum oral liquid according to any one of claims 1 to 7, characterized in that: The steps include: Step S1, washing and processing Astragalus, Polygonatum, Aster, Codonopsis, Atractylodes, Poria, Rehmannia glutinosa, Angelica, Lycium barbarum, Ligustrum lucidum, Eclipta prostrata, and Glycyrrhiza uralensis respectively, extracting effective ingredients by water extraction, alcohol extraction, or water-alcohol combined extraction, and concentrating to prepare an extract; Step S2, weighing the extract according to the component ratio of Huangqi Huangjing oral liquid, adding purified water and stirring to fully dissolve the components, then adding caffeic acid diamine and a preservative and stirring to obtain a solution, and adjusting the pH value of the solution to 4.5-6.5 with a pH adjuster; Step S3, filtering the solution in step S2 with a 0.22-0.45 μm microporous filter membrane for sterilization, and quantitatively filling the filtrate into an oral liquid bottle and sealing it.

9. The method for preparing the Astragalus and Polygonatum oral liquid according to claim 8, characterized in that: In step S1, an ethanol extraction method is used.

10. Use of the Astragalus and Polygonatum oral liquid according to any one of claims 1 to 7 in the preparation of qi-invigorating and blood-tonifying medicines.

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