Processing method of hybrid pennisetum and hybrid broussonetia papyrifera mixed silage

Abstract

A hybrid pennisetum and hybrid broussonetia papyrifera mixed silage is prepared by mixing hybrid pennisetum, hybrid broussonetia papyrifera and sorghum straw as raw materials, adding an additive 1, standing for 3-5 days, adding an additive 2, and fermenting for 35-40 days, the mass ratio of the hybrid pennisetum, the hybrid broussonetia papyrifera and the sorghum straw in the raw materials is (5-7): (2-3): 1, the additive 1 is benzoic acid, and the additive 2 is benzoic acid. The addition amount is 3-5mL / kg of the raw materials. Hybrid pennisetum and hybrid broussonetia papyrifera are used as main raw materials, a small amount of sorghum straw is added and mixed according to a specific proportion, benzoic acid is firstly adopted for treatment, then specific compound lactic acid bacteria are added for fermentation, and the prepared silage has the advantages that the content of acid detergent fibers and neutral detergent fibers is obviously reduced, and the fermentation quality is excellent; the feed has excellent palatability and digestibility and is rich in nutritional value.

Description

Technical Field

[0001] The invention relates to the technical field of silage preparation, and in particular to a processing method for hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage. Background Art

[0002] At present, high-quality forage resources in my country are still scarce, and the development of mixed silage is of great significance for the sustainable development of animal husbandry and agriculture. Mixed silage can improve the nutritional value and utilization efficiency of feed. By mixing different raw materials, the nutritional components can be complemented to provide animals with better quality silage; secondly, this technology can significantly improve the utilization value of agricultural and light industrial by-products. In addition, the promotion of mixed silage will help expand the source of feed, especially in the context of the shortage of feed resources in southern my country, which can be alleviated by developing mixed silage to meet the needs of animal husbandry development. At the same time, this will also help accelerate the development of the agricultural development model of grain-to-feed and the combination of planting and breeding, which is of great significance for the adjustment of agricultural structure.

[0003] The development of mixed silage can also promote ecological protection and reduce environmental pollution caused by agricultural waste, such as reducing air pollution caused by burning straw by converting agricultural byproducts such as straw into feed. At the same time, it also helps to improve agricultural economic benefits, increase farmers' income by improving the utilization value of agricultural byproducts, and promote the extension and value enhancement of the agricultural industry chain.

[0004] In summary, the development of mixed silage can not only provide better quality feed resources for animal husbandry, but also promote the utilization of agricultural by-products, expand feed sources, improve agricultural economic benefits, and promote ecological protection, which plays an important role in promoting the sustainable development of agriculture and animal husbandry in my country. In the process of processing mixed silage, fermentation is an indispensable step, and how to improve the fermentation quality of feed to improve its nutritional value is the key. Summary of the invention

[0005] The invention aims to provide a hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage.

[0006] Another object of the present invention is to provide a processing method for the above-mentioned mixed silage. The mixed silage prepared by mixing hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw in a specific ratio and adding additives has a significantly reduced content of acid detergent fiber and neutral detergent fiber, has excellent palatability and digestibility, and is rich in feed nutritional value.

[0007] The purpose of the present invention is achieved through the following technical solutions: A mixed silage, characterized in that hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw are mixed as raw materials, an additive 1 is added, the mixture is allowed to stand for 3 to 5 days, an additive 2 is added and the mixture is fermented for 35 to 40 days to obtain the mixed silage.

[0008] Furthermore, the mass ratio of hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw in the raw materials is 5-7:2-3:1.

[0009] Further, the additive 1 is benzoic acid, the addition amount is 3-5 mL / kg raw material, and the additive 2 is a fermentation agent, specifically Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1, the addition amount is 1×10 6 CFU / kg raw material.

[0010] A processing method for mixed silage, characterized in that hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw are mixed as raw materials, then an additive 1 is added, the mixture is allowed to stand for 3 to 5 days, and then an additive 2 is added for fermentation for 35 to 40 days.

[0011] Furthermore, the mass ratio of hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw in the raw materials is 5-7:2-3:1.

[0012] Further, the additive 1 is benzoic acid, the addition amount is 3-5 mL / kg raw material, and the additive 2 is a fermentation agent, specifically Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1, the addition amount is 1×10 6 CFU / kg raw material.

[0013] In the addition 2, Lactobacillus pentosus ( Lactobacillus pentosus ) and Pediococcus acidilactici ( Pediococcus acidilactici ) are commercially available and were purchased from China General Microbiological Culture Collection Center. The collection numbers of Lactobacillus pentosus and Pediococcus acidilactici are CGMCC1.3914 and CGMCC1.2696, respectively.

[0014] In the process of preparing silage by mixing hybrid Pennisetum and hybrid Broussonetia, while ensuring the nutritional value, the fermentation quality of the feed is poor, the palatability decreases, and it is not conducive to livestock feeding.

[0015] After hybrid Pennisetum and hybrid paulownia are mixed, a small amount of sorghum straw is added to adjust the initial nutritional structure of the raw materials. Benzoic acid is then used for pretreatment to lower the pH of the system to a range suitable for lactic acid bacteria fermentation. Composite lactic acid bacteria are then inoculated for fermentation. The two lactic acid bacteria cooperate with each other to improve the fermentation effect in the system. During the fermentation process, raw materials with a specific nutritional structure that is more suitable for composite lactic acid bacteria fermentation are used for fermentation metabolism, which improves the nutritional value of the feed while producing more lactic acid to improve the palatability of the feed, and allows green forage to be preserved for a long time. At the same time, the conversion of lactic acid to organic acids such as propionic acid that are not conducive to palatability is inhibited, thereby improving the fermentation quality of the feed.

[0016] Specifically, a method for processing mixed silage is characterized by comprising the following steps: (1) Hybrid Pennisetum, hybrid papyrus and sorghum straw are mixed in a mass ratio of 5-7:2-3:1, crushed into 5-8 cm segments, and then benzoic acid is added in an amount of 3-5 mL / kg. Specifically, benzoic acid is added to distilled water to dissolve into a solution, and then sprayed into the raw materials, mixed and allowed to stand for 3-5 days; (2) Adding additive 2 to the raw material after being processed in step (1), wherein additive 2 is Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1 composition, the addition amount is 1×10 6 CFU / kg raw material, fermented for 35~40 days after mixing.

[0017] The present invention has the following technical effects: In the present invention, hybrid Pennisetum and hybrid Broussonetia papyrifera are used as main raw materials, a small amount of sorghum straw is added, and the mixture is mixed in a specific ratio. The mixture is first treated with benzoic acid, and then specific composite lactic acid bacteria are added for fermentation. The silage prepared has significantly reduced acid detergent fiber and neutral detergent fiber contents, excellent fermentation quality, excellent palatability and digestibility, and rich feed nutritional value. DETAILED DESCRIPTION

[0018] The present invention is described in detail below through examples. It is necessary to point out that the following examples are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Those skilled in the art can make some non-essential improvements and adjustments to the present invention based on the above-mentioned contents of the present invention.

[0019] The Lactobacillus pentosus used in the present invention ( Lactobacillus pentosus ) and Pediococcus acidilactici ( Pediococcus acidilactici ) are commercially available and were purchased from the China General Microbial Culture Collection Center. The collection numbers of Lactobacillus pentosus and Pediococcus acidilactici are CGMCC1.3914 and CGMCC1.2696, respectively. The hybrid Pennisetum used in the present invention is Guimu No. 1, and the hybrid Broussonetia papyrifera is a self-cultivated variety.

[0020] Example 1 A method for processing mixed silage, characterized in that it comprises the following steps: (1) Hybrid Pennisetum, hybrid papyrus and sorghum straw were mixed in a mass ratio of 6:3:1, crushed into 5-8 cm segments, and then benzoic acid was added in an amount of 4 mL / kg. Specifically, benzoic acid was added to distilled water to dissolve into a solution, and then sprayed on the raw materials, mixed and allowed to stand for 4 days; (2) Adding additive 2 to the raw material after being processed in step (1), wherein additive 2 is Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1 composition, the addition amount is 1×10 6 CFU / kg raw material, mixed and bagged to ferment for 35 days.

[0021] During the experiment, we tried to use different organic acids for single treatment (formic acid, benzoic acid, sorbic acid, added at 4 mL / kg raw material), cellulase (added at 30,000 U / kg raw material), composite lactic acid bacteria (Lactobacillus pentosus: Pediococcus acidilactici = 1:1, added at 1×10 6 CFU / kg raw material) were treated as additives, and different organic acids and cellulases were combined with composite lactic acid bacteria for comprehensive treatment. Among them, cellulase treatment was to add cellulase to the raw materials and treat them at a suitable temperature and pH for enzyme for 48 hours.

[0022] After processing, the sensory and nutritional indicators of the mixed silage products are tested respectively. The specific testing steps are as follows: 1. Sensory evaluation According to the DLG scoring method developed by the German Agricultural Association, the sensory quality of silage was scored on site based on three indicators: smell, texture structure and color. According to the scores, it was divided into four levels: level 1 excellent (20-16 points), level 2 fair (15-10 points), level 3 medium (9-5 points), and level 4 corrupt (4-0 points). Multiple parallel tests were set up for each group, and the mean values ​​were calculated. The results are shown in Table 1.

[0023] Table 1: Sensory evaluation data

[0024] It can be seen that the sensory scores of the products treated with formic acid and benzoic acid alone and then naturally fermented were 13.75 and 13.25, respectively, that is, the sensory score of the formic acid group was slightly higher than that of the benzoic acid group, but the sensory scores of the products further inoculated with composite lactic acid bacteria for fermentation on this basis were significantly increased, and the sensory score of the benzoic acid + composite lactic acid bacteria group was slightly higher than that of the formic acid + composite lactic acid bacteria group. After first using cellulase treatment, and then continuing to use benzoic acid treatment in accordance with the steps in Example 1, and then inoculating composite lactic acid bacteria for fermentation, although the sensory scores were higher than those of the single treatment, they were also significantly lower than those of the benzoic acid + composite lactic acid bacteria treatment group.

[0025] 2. Nutritional index determination 100 g samples were taken from the final mixed silage obtained by processing according to the five-point sampling method. After drying, half of the samples were crushed and stored for the determination of neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), dry matter (DM), soluble sugar (WSC) and other indicators. The feed obtained by spraying the feed raw materials without any additives with an equal amount of distilled water and bagging was used as the CK group.

[0026] (1) Dry matter (DM) Weigh 2.00g (m) of dried sample into a weighing bottle (W0) after constant weight, then place it in an oven set at 105±5℃ for 8h, then cool it in a desiccator for 30min and weigh it (W1). The formula is as follows: DM(%)=(W1-W0) / m×100% Where: m——sample weight (g); W0——weight of the weighing bottle after drying at 105±5℃ to constant weight (g); After drying at W1-105±5℃ to constant weight, weigh the bottle and the remaining dry matter (g).

[0027] (2) Soluble sugar (WSC) The WSC content in the extract of the above silage samples was determined by anthrone colorimetry. Take 1 mL of the extract in a dry 10 mL test tube, add 5 mL of anthrone solution, mix well, react in a boiling water bath for 10 minutes, and take out to cool. Measure the absorbance at a wavelength of 620 nm. Weigh 200 mg of anhydrous glucose and dissolve it in 1000 mL of distilled water to prepare a 200 mg / L glucose standard solution. Take different volumes of the standard solution and dilute them to a concentration gradient of 0, 20, 40, 60, 80, and 100 μg / mL. Under the same conditions as above, the reaction is colored, the absorbance is measured, and a standard curve is drawn. Calculate the soluble sugar content in the extract according to the standard curve. The formula is as follows: WSC(%)=(C×V T ×100) / (W×V1×106) In the formula: C-glucose content found from the standard curve; V T -Total volume of sample extract; W-weight of sample; X-V1--Take sample solution during color development.

[0028] (3) Neutral detergent fiber (NDF) 2L of neutral detergent solution: 60g of sodium dodecyl sulfate, 37.22g of disodium ethylenediaminetetraacetate, 13.62g of sodium tetraborate decahydrate, 9.12g of disodium hydrogen phosphate, and 20mL of triethylene glycol. Add 20g of anhydrous sodium sulfite and 4mL of heat-stable α-amylase before use. Add 2L of neutral detergent solution to the ANKOMA200i semi-automatic fiber analyzer, stir and heat. Then stir and rinse with distilled water, soak in acetone, ventilate and evaporate, dry in an oven, and weigh to calculate the NDF content. The formula is as follows: NDF(%DM)=(m2-m1×C1) / m×100% In the formula: m1-mass of empty filter bag; m2--mass of sample residue and filter bag; m---sample weight; C1 is the correction factor of the blank bag (mass after drying / original mass).

[0029] (4) Acid detergent fiber (ADF) Acidic detergent solution: Weigh 40g of hexadecyltrimethylammonium bromide and dissolve it in 2L of 1.00N sulfuric acid solution. Add 2L of acidic detergent solution to the ANKOMA200i semi-automatic fiber analyzer, stir and heat. Then stir and rinse with distilled water, soak with acetone, volatilize with ventilation, dry in an oven, and weigh to calculate the ADF content. The formula is as follows: ADF(%DM)=(m2-m1×C1) / m×100% Where: m1--mass of empty filter bag; m2---mass of sample residue and filter bag; m---sample weight; C1 is the correction factor of the blank bag (mass after drying / original mass).

[0030] (5) Crude protein (CP) The CP content is determined by the method in GB / T 6432-2018 using a KjeltecTM8100 Kjeldahl nitrogen analyzer. 0.4 g of copper sulfate and 6 g of anhydrous potassium sulfate are crushed and mixed thoroughly. 0.5-1 g of the sample is weighed into a digestion tube, and the mixed copper sulfate and potassium sulfate are added. Then 10 mL of concentrated sulfuric acid is added and digested in a 420°C digestion furnace for about 1.5 h to convert protein substances into ammonia, and then ammonium sulfate is generated under the action of sulfuric acid. After the sample is cooled (the sample color is clear or blue-green), 50 mL of 40% NaOH solution and 50 mL of distilled water are added for distillation, and 1% boric acid solution is used for absorption to finally generate ammonium borate. After adding 2 drops of methyl red-bromocresol green indicator to the boric acid solution, it is titrated with calibrated 0.1M hydrochloric acid to calculate the CP content. The TN content is the CP content multiplied by 6.25.

[0031] The changes in the nutritional components of the raw materials before and after processing are shown in Table 2.

[0032] Table 2: Changes in nutritional composition of silage with different processing methods

[0033] As can be seen from the table above, in the CK group without any treatment, the acid detergent fiber and neutral detergent fiber content were higher, the crude protein was lower, and the soluble sugar content was also higher. After treatment with a single organic acid, enzyme or compound lactic acid bacteria, the dry matter, acid detergent fiber, neutral detergent fiber and soluble sugar all showed a significant decrease, while the crude protein was significantly increased compared with the CK group. After two-step treatment, the acid detergent fiber and neutral detergent fiber in the benzoic acid + compound lactic acid bacteria group showed a significant decrease, the crude protein was significantly increased, and the soluble sugar content was also significantly increased compared with the single treatment group. In the single treatment group, the formic acid group, which was in a relatively excellent position, showed a downward trend in overall nutritional value after the compound treatment with compound lactic acid bacteria, and the crude protein and soluble sugar content were both lower than those of the single formic acid treatment group.

[0034] 3. Determination of fermentation quality Open the bag and take out the silage for fermentation quality determination: take 10g of sample, add 90mL of distilled water, stir evenly, extract at 50℃, and then filter to make an extract. Put the extract at 4℃, refrigerate for 24h, measure pH, ammonia nitrogen (AN), and determine the content of organic acids (lactic acid LA, acetic acid AA, propionic acid PA) by liquid chromatography. The results are shown in Table 3.

[0035] Table 3: Changes in fermentation quality of silage with different processing

[0036] As can be seen from the above table, the pH of the silage treated with single organic acid, cellulase and compound lactic acid bacteria was slightly lower than that of the CK group without any treatment, and the lactic acid production was higher in the formic acid group and the compound lactic acid bacteria group, but the propionic acid produced in the organic acid treatment group was higher, which shows that more propionic acid was synthesized in the organic acid treatment group, which can make the feed produce an unpleasant odor, reducing the palatability of the feed and affecting animal feeding. In the composite treatment, the pH of the fermented feed obtained after multi-step treatment further decreased, and the ammonia nitrogen content further increased, indicating that the priority treatment of organic acids quickly reduced the pH of the raw materials. After further inoculation of compound lactic acid bacteria, the lactic acid bacteria quickly started to grow, effectively improving the fermentation effect and the fermentation quality of the feed. Among them, the lactic acid content of the benzoic acid + compound lactic acid bacteria treatment group was significantly higher, and the propionic acid content was the lowest among all treatment groups, second only to the CK group, and lower than the single benzoic acid and single compound lactic acid bacteria treatment groups. This shows that the two-step treatment of benzoic acid and compound lactic acid bacteria during the fermentation process produced a synergistic effect, which inhibited the synthesis of propionic acid during the fermentation process and improved the palatability of the feed.

[0037] During the experiment, we tried to process different raw materials: By processing hybrid pennisetum and hybrid paper mulberry in different proportions, as well as the combination of hybrid pennisetum, hybrid paper mulberry and sorghum straw, respectively, when hybrid pennisetum and hybrid paper mulberry were mixed as raw materials, the mass ratio of hybrid pennisetum and hybrid paper mulberry was changed to test the influence of different raw material proportions on the nutritional value and fermentation quality of the silage obtained by processing, and on this basis, sorghum straw was further added, and the nutritional value and fermentation quality of the silage fermented under each ratio were tested.

[0038] The changes in nutritional components of silage obtained by processing raw materials in different proportions are shown in Table 4, and the fermentation quality is shown in Table 5. In the data, A represents hybrid paulownia, B represents hybrid pennisetum, and C represents sorghum straw.

[0039] Table 4: The changes in nutritional composition of feed with different raw material ratios are shown in the following table.

[0040]

[0041] As can be seen from the above table, different raw material compositions will lead to differences in the initial nutrient content of the raw materials. In the subsequent processing, lactic acid bacteria fermentation will also produce different nutritional environments, resulting in great differences in the microbial fermentation process. In the process of mixing hybrid paulownia and hybrid foxtail grass as raw materials, when the mass ratio of hybrid paulownia and hybrid foxtail grass is 7:3, the nutritional value of the fermented silage is the highest, and its acid detergent fiber and neutral detergent fiber are significantly lower than other ratios, while the crude protein and soluble sugar contents are significantly higher than other ratios. As the proportion of hybrid paulownia decreases, the overall nutritional value of the feed gradually decreases. On this basis, when sorghum straw is further added, it is found that when the proportion of hybrid paulownia is small, the nutritional value of the fermented feed is further improved.

[0042] Table 5: Changes in fermentation quality of feed with different raw material ratios

[0043] When the mass ratio of hybrid paulownia and hybrid foxtail grass is 7:3, the nutritional value of the prepared silage is optimal, which is significantly better than the silage prepared by fermentation of single hybrid foxtail grass or single hybrid paulownia, but the fermentation quality effect is not ideal. Although the nutritional value of the fermented silage is higher than that of other ratios when the proportion of hybrid paulownia is higher than that of hybrid foxtail grass, the fermentation quality of the feed is not optimal. The most propionic acid is produced during the fermentation process, which can cause the feed to produce a foul odor. Moreover, as the proportion of hybrid paulownia decreases, the content of propionic acid does not decrease significantly, and the content of lactic acid produced to improve the palatability of the feed does not change significantly, indicating that there is a situation where lactic acid is used as a substrate to convert to propionic acid. On this basis, after further adding a small amount of sorghum straw, it was found that the lactic acid production in the fermented feed increased significantly, while the acetic acid and propionic acid contents decreased significantly. This shows that the addition of sorghum straw changes the nutritional structure of the initial raw materials and provides a more suitable environment for the fermentation of microorganisms. In this process, the conversion of lactic acid as a substrate to propionic acid is inhibited to a certain extent.

[0044] Example 2 A method for processing mixed silage, characterized in that it comprises the following steps: (1) Hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw were mixed in a mass ratio of 5:2.5:1, crushed into 5-8 cm segments, and then benzoic acid was added in an amount of 5 mL / kg. Specifically, benzoic acid was added to distilled water to dissolve into a solution, and then sprayed on the raw materials, mixed and allowed to stand for 3 days; (2) Adding additive 2 to the raw material after being processed in step (1), wherein additive 2 is Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1 composition, the addition amount is 1×10 6CFU / kg raw material, mixed and fermented for 40 days.

[0045] The nutritional components and fermentation quality data of the silage of this example are shown in Table 6.

[0046] Table 6:

[0047] It can be seen that the mixed silage prepared in this embodiment has excellent palatability and digestibility, and is rich in feed nutritional value.

[0048] Example 3 A method for processing mixed silage, characterized in that it comprises the following steps: (1) Hybrid Pennisetum, hybrid papyrus and sorghum straw were mixed in a mass ratio of 7:2:1, crushed into 5-8 cm segments, and then benzoic acid was added in an amount of 3 mL / kg. Specifically, benzoic acid was added to distilled water to dissolve into a solution, and then sprayed on the raw materials, mixed and allowed to stand for 5 days; (2) Adding additive 2 to the raw material after being processed in step (1), wherein additive 2 is Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1 composition, the addition amount is 1×10 6 CFU / kg raw material, mixed and fermented for 38 days.

[0049] The nutritional components and fermentation quality data of the silage of this example are shown in Table 7.

[0050] Table 7:

[0051] The mixed silage prepared in this embodiment has excellent palatability and digestibility, and the feed has rich nutritional value. During the preparation process, we tried to use Lactobacillus plantarum (CGMCC1.1856) to replace the lactic acid Pediococcus in the composite lactic acid bacteria, and the remaining steps were the same as in Example 3. It was found that the other nutritional components of the prepared silage did not change much, but the content of soluble sugar decreased, and the organic acid content was also significantly different. The lactic acid content decreased to 5.47%, and the acetic acid and propionic acid contents increased significantly, which were 1.52% and 0.57%, respectively.

Claims

1. A mixed silage, characterized in that: The raw materials are hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum stalks, which are mixed, and then additive 1 is added. After standing for 3 to 5 days, additive 2 is added and fermented for 35 to 40 days. The mass ratio of hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum stalks in the raw materials is 5 to 7:2 to 3:

1. The additive 1 is benzoic acid, and the addition amount is 3 to 5 mL / kg of raw material.

2. The mixed silage according to claim 1, characterized in that: The additive 2 is a fermentation agent, specifically Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1, the addition amount is 1×10 6 CFU / kg raw material.

3. A method for processing hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage, characterized in that: The raw materials are hybrid Pennisetum, hybrid Broussonetia chinensis and sorghum straw, and then additive 1 is added. After standing for 3 to 5 days, additive 2 is added for fermentation.

4. The method for processing hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage according to claim 3, characterized in that: The mass ratio of hybrid Pennisetum, hybrid Broussonetia papyrifera and sorghum straw in the raw materials is 5-7:2-3:

1.

5. The method for processing hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage according to claim 3 or 4, characterized in that: The additive 1 is benzoic acid, the addition amount is 3-5 mL / kg raw material, and the additive 2 is a fermentation agent, specifically Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1, the addition amount is 1×10 6 CFU / kg raw material.

6. A method for processing hybrid Pennisetum and hybrid Broussonetia papyrifera mixed silage, characterized in that: The steps include: (1) Hybrid Pennisetum, hybrid papyrus and sorghum straw are mixed in a mass ratio of 5-7:2-3:1, crushed into 5-8 cm segments, and then benzoic acid is added in an amount of 3-5 mL / kg. Specifically, benzoic acid is added to distilled water to dissolve into a solution, and then sprayed into the raw materials, mixed and allowed to stand for 3-5 days; (2) Adding additive 2 to the raw material after being processed in step (1), wherein additive 2 is Lactobacillus pentosus ( Lactobacillus pentosus ): Pediococcus acidilactici ( Pediococcus acidilactici )=1:1 composition, the addition amount is 1×10 6 CFU / kg raw material, fermented for 35~40 days after mixing.

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