Probiotic tea and preparation method thereof

Abstract

The invention relates to probiotic tea and a preparation method thereof, and belongs to the technical field of tea drinks, and the probiotic tea comprises the following raw material components in parts by weight: oolong tea, pyracantha fortuneana fruit-blue medicine water lily extract, hawthorn pectin, chrysanthemum and gardenia. The prepared probiotic tea has a good anti-fatigue effect, meanwhile, the probiotic tea can reduce generation of precipitates, the turbidity of tea liquid is effectively improved, and the probiotic tea is more convenient to store for a long time.

Description

Technical Field

[0001] The invention belongs to the technical field of tea beverages, and in particular relates to a probiotic tea and a preparation method thereof. Background Art

[0002] Oolong tea is rich in natural tea polyphenols, catechins, caffeine and other effective ingredients. It has multiple functions such as enhancing immunity, promoting the growth of probiotics, and regulating intestinal flora.

[0003] In the prior art, oolong tea is used in combination with other Chinese medicinal components to obtain a probiotic tea that has both nutritional and health effects and is refreshing and thirst-quenching. However, in actual production, due to the addition of Chinese medicinal components, the sensory quality and health functions of probiotic tea are often not taken into account. If the concentration of the tea soup is too thin, it cannot well reflect the flavor and aroma of the original tea. If the tea soup is too thick, as the leaching rate of tea polyphenols and other substances increases, they will react with the effective ingredients of Chinese medicinal materials to generate precipitation, resulting in a larger turbidity of the probiotic tea, affecting the perception and quality of the tea drink.

[0004] In addition, people in modern cities are prone to chronic fatigue, decreased immunity and other symptoms, i.e. sub-health status, due to work pressure, frequent late night work or irregular diet and work and rest. Therefore, it is of great significance to develop a tea-flavored beverage that can improve the sub-health status of the human body and meet the sensory quality requirements. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention provides a probiotic tea and a preparation method thereof.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0009] The raw materials of a probiotic tea include the following components in parts by weight: 8-12 parts of oolong tea, 0.1-0.15 parts of pyracantha fruit-blue water lily extract, 0.3-0.5 parts of hawthorn pectin, 0.5-1 parts of chrysanthemum and 0.5-1 parts of gardenia.

[0010] Furthermore, the preparation method of the Pyracantha fortuneana fruit-blue water lily extract comprises the following steps:

[0011] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0012] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 10 to 15 times the weight of the powder mixture, each time for 1 to 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract having a relative density of 1.20 to 1.30 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0013] S3: The residue obtained in S2 is extracted twice with water 5 to 10 times the weight of the powder mixture by heating and refluxing, each time for 2 to 3 hours, the two filtrates are combined, allowed to stand for 3 to 5 hours, filtered, and concentrated to an extract with a relative density of 1.20 to 1.30 to obtain the second extract;

[0014] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0015] Furthermore, the preparation method of hawthorn pectin comprises the following steps:

[0016] Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, cut it into pieces and remove the seeds. Then, soak it in 4 to 6 parts of 60 to 80% ethanol solution by volume for 10 to 20 hours. After the soaking, the solid and liquid are separated. The liquid part is clarified and filtered to obtain a hawthorn extract.

[0017] Q2, the solid part obtained in Q1 is put into pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 2 to 4 hours, it is heated to 50 to 70°C, and a decomposing enzyme is added to the slurry. After enzymolysis for 3 to 5 hours, it is squeezed and filtered to obtain hawthorn juice;

[0018] Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 0.5 to 1 hour, separate the pectin to obtain crude pectin, dehydrate and dry to obtain hawthorn pectin.

[0019] Furthermore, the decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:1.

[0020] Furthermore, the concentration of the decomposing enzyme is 0.5-2 g / kg.

[0021] A method for preparing probiotic tea, characterized in that it comprises the following steps:

[0022] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:20-30, the extraction temperature is 80-90°C, and the extraction time is 15-25min;

[0023] T2, add hawthorn pectin to 3-5 times the weight of the drug in pure water, heat to 40-50°C, add Pyracantha fruit-blue water lily extract, stir for 0.5-1h to obtain auxiliary liquid;

[0024] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0025] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0026] 1. The pyracantha fruit of the present invention has significant medicinal value, is rich in vitamin C and other antioxidants, is helpful to enhance the immune system, improve body resistance, and is also helpful to resist the damage of free radicals, delay the aging process, and keep the skin healthy and lustrous; the pyracantha fruit is rich in potassium, which is helpful to regulate blood pressure; the blue medicinal water lily is both medicinal and edible, and has rich nutritional value, containing anthocyanins, various flavonoids and phenolic acid active substances, as well as various amino acids, vitamins, iron, selenium and other trace elements. These nutrients have a good conditioning effect on the human body, and have multiple functions such as removing free radicals in the body and inhibiting lipid peroxidation, anti-cancer and anti-aging, preventing diabetes, and losing weight.

[0027] The present invention comprehensively utilizes natural components of oolong tea and pyracantha fruit-blue water lily extract and other substances, and effectively improves the turbidity of the tea liquid on the basis of fully retaining the rich aroma of the original oolong tea. At the same time, it has the health care effects of enhancing human immune function, eliminating free radicals and relieving fatigue, and can significantly improve the health status of sub-healthy people.

[0028] 2. The present invention combines the raw materials according to a specific ratio, and extracts the effective components of blue water lily and pyracantha fruit in particular, so that the two can work together to exert health-care effects. In addition, hawthorn pectin is used to separately treat the pyracantha fruit-blue water lily extract to avoid and reduce its interaction with tea polyphenols and other substances, thereby reducing the generation of precipitation and effectively improving the turbidity of the tea liquid. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Example 1

[0031] A probiotic tea, wherein the raw materials include the following components by weight: 10 parts of oolong tea, 0.12 parts of pyracantha fruit-blue water lily extract, 0.3 parts of hawthorn pectin, 0.8 parts of chrysanthemum and 0.8 parts of

[0032] Gardenia, and its preparation method is as follows.

[0033] 1. Preparation of Pyracantha fortuneana fruit-blue water lily extract, the preparation method is as follows:

[0034] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0035] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the powder mixture, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0036] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2 hours, and the two filtrates were combined, allowed to stand for 4 hours, filtered, and concentrated to an extract with a relative density of 1.25 to obtain the second extract;

[0037] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0038] 2. Preparation of hawthorn pectin, comprising the following steps:

[0039] Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, it is cut into pieces and deseeded, and then soaked in 5 parts of 70% ethanol solution by volume for 15 hours. After the soaking, the solid and liquid are separated, and the liquid part is clarified and filtered to obtain a hawthorn extract;

[0040] Q2, the solid part obtained in Q1 is put into pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 3 hours, it is heated to 60°C, and a decomposing enzyme is added to the slurry. After enzymolysis for 4 hours, it is squeezed and filtered to obtain hawthorn juice, wherein the concentration of the decomposing enzyme is 1g / kg, and the decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:1;

[0041] Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 1 hour, separate the pectin to obtain crude pectin, dehydrate and dry to obtain hawthorn pectin.

[0042] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0043] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:25, the extraction temperature is 85°C, and the extraction time is 20min;

[0044] T2, add hawthorn pectin to pure water 4 times the weight of the drug, heat to 45°C, add Pyracantha fruit-blue water lily extract, stir for 0.5-1h to obtain auxiliary liquid;

[0045] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0046] Example 2

[0047] A probiotic tea, wherein the raw materials include the following components by weight: 8 parts of oolong tea, 0.1 parts of pyracantha fruit-blue water lily extract, 0.3 parts of hawthorn pectin, 0.5 parts of chrysanthemum and 0.5 parts of gardenia, and the preparation method thereof is as follows.

[0048] 1. Preparation of Pyracantha fortuneana fruit-blue water lily extract, the preparation method is as follows:

[0049] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0050] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the powder mixture, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0051] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2 hours, and the two filtrates were combined, allowed to stand for 3 hours, filtered, and concentrated to an extract with a relative density of 1.20 to obtain the second extract;

[0052] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0053] 2. Preparation of hawthorn pectin, comprising the following steps:

[0054] Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, it is cut into pieces and deseeded, and then soaked in 4 parts of 60% ethanol solution by volume for 10 hours. After the soaking, the solid and liquid are separated, and the liquid part is clarified and filtered to obtain a hawthorn extract;

[0055] Q2, the solid part obtained in Q1 is put into pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 2 hours, it is heated to 50°C, and a decomposing enzyme is added to the slurry. After enzymolysis for 3 hours, it is squeezed and filtered to obtain hawthorn juice, wherein the concentration of the decomposing enzyme is 0.5g / kg, and the decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:1;

[0056] Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 0.5 h, separate the pectin to obtain crude pectin, and dehydrate and dry to obtain hawthorn pectin.

[0057] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0058] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:20, the extraction temperature is 80°C, and the extraction time is 15min;

[0059] T2, add hawthorn pectin to pure water 3 times the weight of the drug, heat to 40°C, add Pyracantha fruit-blue water lily extract, stir for 0.5h, and obtain auxiliary liquid;

[0060] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0061] Example 3

[0062] A probiotic tea, wherein the raw materials include the following components by weight: 12 parts of oolong tea, 0.15 parts of pyracantha fruit-blue water lily extract, 0.5 parts of hawthorn pectin, 1 part of chrysanthemum and 1 part of gardenia, and the preparation method thereof is as follows.

[0063] 1. Preparation of Pyracantha fortuneana fruit-blue water lily extract, the preparation method is as follows:

[0064] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0065] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the powder mixture, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0066] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 3 hours, and the two filtrates were combined, allowed to stand for 5 hours, filtered, and concentrated to an extract with a relative density of 1.30 to obtain the second extract;

[0067] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0068] 2. Preparation of hawthorn pectin, comprising the following steps:

[0069] Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, the raw material is cut into pieces and the seeds are removed. The raw material is then soaked in 6 parts of ethanol solution with a volume concentration of 80% for 20 hours. After the soaking, the solid and liquid are separated, and the liquid part is clarified and filtered to obtain a hawthorn extract;

[0070] Q2, the solid part obtained in Q1 is put into pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 4 hours, it is heated to 50-70°C, and a decomposing enzyme is added to the slurry. After enzymolysis for 5 hours, it is squeezed and filtered to obtain hawthorn juice, wherein the concentration of the decomposing enzyme is 2g / kg, and the decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:1;

[0071] Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 1 hour, separate the pectin to obtain crude pectin, dehydrate and dry to obtain hawthorn pectin.

[0072] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0073] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:30, the extraction temperature is 90°C, and the extraction time is 25min;

[0074] T2, add hawthorn pectin to pure water 5 times the weight of the drug, heat to 50°C, add Pyracantha fruit-blue water lily extract, stir for 1 hour to obtain auxiliary liquid;

[0075] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0076] Example 4

[0077] A probiotic tea, wherein the raw materials include the following components by weight: 9 parts of oolong tea, 0.11 parts of pyracantha fruit-blue water lily extract, 0.35 parts of hawthorn pectin, 0.6 parts of chrysanthemum and 0.5-1 parts of gardenia, and the preparation method thereof is as follows.

[0078] 1. Preparation of Pyracantha fortuneana fruit-blue water lily extract, the preparation method is as follows:

[0079] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0080] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the powder mixture, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0081] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2.3 hours, the two filtrates were combined, allowed to stand for 3.5 hours, filtered, and concentrated to an extract with a relative density of 1.23 to obtain the second extract;

[0082] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0083] 2. Preparation of hawthorn pectin, comprising the following steps:

[0084] Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, cut it into pieces and remove the seeds. Then, soak it in 4.5 parts of 65% ethanol solution by volume for 12 hours. After soaking, the solid and liquid are separated. The liquid part is clarified and filtered to obtain a hawthorn extract.

[0085] Q2, the solid part obtained in Q1 is put into pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 2.5 hours, it is heated to 55°C, and a decomposing enzyme is added to the slurry. After enzymolysis for 3.5 hours, it is squeezed and filtered to obtain hawthorn juice, wherein the concentration of the decomposing enzyme is 0.9 g / kg, and the decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:1;

[0086] Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 0.6h, separate the pectin to obtain crude pectin, dehydrate and dry to obtain hawthorn pectin.

[0087] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0088] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:23, the extraction temperature is 83°C, and the extraction time is 18min;

[0089] T2, add hawthorn pectin to 3.5 times the weight of the drug in pure water, heat to 42°C, add Pyracantha fruit-blue water lily extract, stir for 0.6h, and obtain auxiliary liquid;

[0090] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0091] Comparative Example 1

[0092] The only difference from Example 1 is that no Pyracantha fruit is added in the preparation of the Pyracantha fruit-Blue Water Lily extract.

[0093] A probiotic tea, wherein the raw materials include the following components in parts by weight: 10 parts of oolong tea, 0.12 parts of blue water lily extract, 0.3 parts of hawthorn pectin, 0.8 parts of chrysanthemum and 0.8 parts of gardenia, and the preparation method thereof is as follows.

[0094] 1. Preparation of blue water lily extract, the preparation method is as follows:

[0095] S1. Crush the blue medicinal water lily with a grinder and pass it through a 20-mesh sieve to obtain blue medicinal water lily powder;

[0096] S2, the blue medicinal water lily powder obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the blue medicinal water lily powder, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining medicinal residue is reserved for later use;

[0097] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2 hours, and the two filtrates were combined, allowed to stand for 4 hours, filtered, and concentrated to an extract with a relative density of 1.25 to obtain the second extract;

[0098] S4, evenly mixing the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray drying the mixture to obtain a blue water lily extract.

[0099] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0100] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:25, the extraction temperature is 85°C, and the extraction time is 20min;

[0101] T2, add hawthorn pectin to pure water 4 times the weight of the drug, heat to 45°C, add blue water lily extract, stir for 0.5-1h to obtain auxiliary solution;

[0102] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0103] Comparative Example 2

[0104] The only difference from Example 1 is that in the preparation of the Pyracantha fruit-blue water lily extract, blue water lily is not added.

[0105] A probiotic tea, wherein the raw materials include the following components in parts by weight: 10 parts of oolong tea, 0.12 parts of pyracantha fruit extract, 0.3 parts of hawthorn pectin, 0.8 parts of chrysanthemum and 0.8 parts of gardenia, and the preparation method thereof is as follows.

[0106] 1. Preparation of Pyracantha fortuneana fruit extract, the preparation method thereof is as follows:

[0107] S1. Grind the Pyracantha fruit with a grinder and pass it through a 20-mesh sieve to obtain Pyracantha fruit powder;

[0108] S2, the pyracantha fruit powder obtained in S1 was extracted twice with a 50% ethanol solution with a volume concentration of 15 times that of the pyracantha fruit powder, each time for 2 hours, filtered, the two ethanol extracts were combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining dregs were reserved;

[0109] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2 hours, and the two filtrates were combined, allowed to stand for 4 hours, filtered, and concentrated to an extract with a relative density of 1.25 to obtain the second extract;

[0110] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain the Pyracantha fortuneana fruit extract.

[0111] 3. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0112] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:25, the extraction temperature is 85°C, and the extraction time is 20min;

[0113] T2, add hawthorn pectin to pure water 4 times the weight of the drug, heat to 45°C, add pyracantha fruit extract, stir for 0.5-1h to obtain auxiliary liquid;

[0114] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0115] Comparative Example 3

[0116] The only difference from Example 1 is that the Pyracantha fortuneana fruit-blue water lily extract is not added to the raw materials of the probiotic tea.

[0117] A probiotic tea, wherein the raw materials include the following components in parts by weight: 10 parts of oolong tea, 0.3 parts of hawthorn pectin, 0.8 parts of chrysanthemum and 0.8 parts of gardenia, and the preparation method thereof is as follows.

[0118] The preparation of probiotic tea using the above raw materials comprises the following steps:

[0119] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:25, the extraction temperature is 85°C, and the extraction time is 20min;

[0120] T2. Add hawthorn pectin to pure water 4 times the weight of the drug, heat to 45°C, and stir for 0.5-1h to obtain auxiliary liquid;

[0121] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0122] Comparative Example 4

[0123] The only difference from Example 1 is that no hawthorn pectin is added in the preparation of the probiotic tea.

[0124] A probiotic tea, wherein the raw materials include the following components in parts by weight: 10 parts of oolong tea, 0.12 parts of pyracantha fruit-blue water lily extract, 0.8 parts of chrysanthemum and 0.8 parts of gardenia, and the preparation method thereof is as follows.

[0125] 1. Preparation of Pyracantha fortuneana fruit-blue water lily extract, the preparation method is as follows:

[0126] S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture;

[0127] S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 15 times the weight of the powder mixture, each time for 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract with a relative density of 1.25 to obtain an extract 1, and the remaining drug residue is reserved for later use;

[0128] S3: The residue obtained in S2 was extracted twice with water 10 times the weight of the powder mixture by heating and refluxing, each time for 2 hours, and the two filtrates were combined, allowed to stand for 4 hours, filtered, and concentrated to an extract with a relative density of 1.25 to obtain the second extract;

[0129] S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

[0130] 2. Using the above raw materials to prepare probiotic tea, the process includes the following steps:

[0131] T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:25, the extraction temperature is 85°C, and the extraction time is 20min;

[0132] T2, add hawthorn pectin to pure water 4 times the weight of the drug, heat to 45°C, add Pyracantha fruit-blue water lily extract, stir for 0.5-1h to obtain auxiliary liquid;

[0133] T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.

[0134] Product performance testing

[0135] 1. Mouse swimming test method: SPF mice, half male and half female, a total of 90 mice, weighing 20±2g, were randomly divided into 9 groups, 10 mice in each group, respectively, Examples 1 to 4 and Comparative Examples 1 to 5, where Comparative Example 5 was a blank control group. The probiotic tea beverages prepared in Examples 1 to 4 and Comparative Examples 1 to 4 were administered to mice by gavage at a dose of 10mL / kg, and the blank control group was given the same volume of normal saline. The frequency and time of administration of each group of mice were once a day for 30 consecutive days. One hour after the administration on the 30th day, each group of animals was placed in a swimming tank at 10°C, and the time for each group of mice to sink to the bottom of the water was observed as the continuous swimming time. The test results are shown in Table 1.

[0136] 2. Turbidity determination: The probiotic tea obtained in Examples 1 to 4 and Comparative Examples 1 to 3 was packaged in a fixed volume and stored in the dark at 15 to 25°C. After standing for 6 months, the turbidity of the tea liquid was measured. The test results are shown in Table 1. Turbidity is the degree of obstruction of suspended matter in water to the transmission of light. 1 mg of silicon dioxide in 1 L of distilled water is one turbidity unit. A large turbidity indicates that there are many suspended matter in the tea soup and poor brightness.

[0137] Table 1

[0138] Continuous swimming time / min Turbidity (NTU) Example 1 12.2 2.57 Example 2 11.8 2.59 Example 3 12.0 2.64 Example 4 11.7 2.61 Comparative Example 1 9.8 2.56 Comparative Example 2 10.3 2.64 Comparative Example 3 8.6 2.21 Comparative Example 4 12.0 9.27 Comparative Example 5 7.8 ——

[0139] Combining the contents of Examples 1 to 5 with the contents in Table 1, it can be seen that after the mice drank the probiotic tea prepared by the present invention, their continuous swimming time exceeded 11.7 minutes, which was relatively long, indicating that the probiotic tea prepared by the present invention had a good anti-fatigue effect. The probiotic tea prepared by the technical scheme of the present invention was stored in the dark at 15 to 25°C. After standing for 6 months, the turbidity test data was lower than 2.64, and the turbidity was relatively low, indicating that the probiotic tea prepared by the present invention can reduce the generation of precipitation, effectively improve the turbidity of the tea liquid, and is easier to store for a long time.

[0140] From the above content, it can be seen that comparative example 5 is a blank control group, and mice are given the same volume of normal saline.

[0141] In the preparation process of the probiotic tea of ​​Comparative Example 1 and Comparative Example 2, Pyracantha fruit and blue medicinal water lily were not added respectively. After the mice drank the probiotic tea prepared in Comparative Example 1 and Comparative Example 2, the continuous swimming time of the mice was 9.8 minutes and 10.3 minutes respectively, which were both lower than the continuous swimming time of 12.2 minutes after the mice drank the probiotic tea prepared in Example 1. In the preparation of the probiotic tea of ​​Comparative Example 3, neither Pyracantha fruit nor blue medicinal water lily was added. After the mice drank the probiotic tea prepared in Comparative Example 3, the swimming test result of the mice was 8.6 minutes, which was less than the anti-fatigue result of the probiotic tea in Example 1 and less than the anti-fatigue result of the probiotic tea in Comparative Example 1 and Comparative Example 2. The results show that Pyracantha fruit and blue medicinal water lily have a synergistic effect in improving the anti-fatigue effect of the prepared probiotic tea.

[0142] The turbidity test result of the probiotic tea prepared in Comparative Example 3 is worse than that of the probiotic tea prepared in Example 1. Combined with Example 1, it can be seen that the Pyracantha fruit-Blue Water Lily extract can reduce the production of precipitation in the prepared probiotic tea and improve the transparency of the probiotic tea.

[0143] No hawthorn pectin was added to the probiotic tea prepared in Comparative Example 4. The probiotic tea of ​​Comparative Example 4 was stored in the dark at 15-25° C., and the turbidity test data after standing for 6 months was significantly greater than the turbidity test data of the probiotic tea prepared in Example 1. This shows that the addition of hawthorn pectin can significantly reduce the generation of precipitation, significantly improve the turbidity of the tea liquid, and significantly improve the brightness of the tea liquid after standing for 6 months.

[0144] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A probiotic tea, characterized in that: The raw materials include the following components in parts by weight: 8 to 12 parts of oolong tea, 0.1 to 0.15 parts of pyracantha fruit-blue water lily extract, 0.3 to 0.5 parts of hawthorn pectin, 0.5 to 1 parts of chrysanthemum and 0.5 to 1 parts of gardenia.

2. A probiotic tea according to claim 1, characterized in that: The preparation method of the Pyracantha fortuneana fruit-blue water lily extract comprises the following steps: S1. Mix Pyracantha fruit and blue water lily in a mass ratio of 1:1, then grind them with a grinder and pass them through a 20-mesh sieve to obtain a powder mixture; S2, the powder mixture obtained in S1 is subjected to heating and reflux extraction twice with a 50% ethanol solution having a volume concentration of 10 to 15 times the weight of the powder mixture, each time for 1 to 2 hours, filtered, the two ethanol extracts are combined, and concentrated under reduced pressure to an extract having a relative density of 1.20 to 1.30 to obtain an extract 1, and the remaining drug residue is reserved for later use; S3: The residue obtained in S2 is extracted twice with water 5 to 10 times the weight of the powder mixture by heating and refluxing, each time for 2 to 3 hours, the two filtrates are combined, allowed to stand for 3 to 5 hours, filtered, and concentrated to an extract with a relative density of 1.20 to 1.30 to obtain the second extract; S4. Evenly mix the extract extract 1 prepared in S2 and the extract extract 2 prepared in S3 to obtain a total extract extract, and spray-dry the mixture to obtain a Pyracantha fortuneana-blue water lily extract.

3. The probiotic tea according to claim 1, characterized in that: The preparation method of hawthorn pectin comprises the following steps: Q1. By weight, 1 part of fresh hawthorn fruit is used as raw material. After washing the raw material, cut it into pieces and remove the seeds. Then, soak it in 4 to 6 parts of 60 to 80% ethanol solution by volume for 10 to 20 hours. After the soaking, the solid and liquid are separated. The liquid part is clarified and filtered to obtain a hawthorn extract. Q2, the solid part obtained in Q1 is placed in pure water, wherein the mass ratio of the solid part to the pure water is 1:1, and the pre-treated slurry is obtained. After leaching for 2 to 4 hours, it is heated to 50 to 70° C., a decomposing enzyme is added to the slurry, and after enzymolysis for 3 to 5 hours, it is squeezed and filtered to obtain hawthorn juice; Q3. Mix the hawthorn juice obtained in Q2 with the hawthorn extract obtained in Q1, stir for 0.5 to 1 hour, separate the pectin to obtain crude pectin, dehydrate and dry to obtain hawthorn pectin.

4. A beneficial tea according to claim 3, characterized in that: The decomposing enzyme is a mixture of pectinase and cellulase in a mass ratio of 2:

1.

5. A beneficial tea according to claim 4, characterized in that: The concentration of the decomposing enzyme is 0.5-2 g / kg.

6. A method for preparing the beneficial tea according to any one of claims 1 to 5, characterized in that: The following steps are involved: T1. Weigh the raw materials according to weight, add oolong tea, chrysanthemum and gardenia into pure water, heat and extract, and filter the extract after cooling to obtain tea liquid, wherein the mass ratio of oolong tea, chrysanthemum, gardenia and total weight to pure water during the extraction process is 1:20-30, the extraction temperature is 80-90°C, and the extraction time is 15-25min; T2, add hawthorn pectin to 3-5 times the weight of the drug in pure water, heat to 40-50°C, add Pyracantha fruit-blue water lily extract, stir for 0.5-1h to obtain auxiliary liquid; T3. Evenly mix the tea clear liquid in T1 and the auxiliary liquid in T2, stir evenly, and filter finely to obtain probiotic tea.