Anti-aging composition, application and cosmetic

Abstract

The invention belongs to the technical field of cosmetic production, and discloses an anti-aging composition which comprises a nymphaea tetragona flower extract and vegetable oil, and the mass ratio of the nymphaea tetragona flower extract to the vegetable oil is (0.5-2): 4. The vegetable oil is selected from at least one of sunflower seed oil, peony seed oil, olive fruit oil, prunus amygdalus dulcis oil, linseed oil and borage seed oil, and the vegetable oil at least contains the sunflower seed oil. The invention discloses an anti-aging composition capable of delaying skin aging, and in addition, the invention further discloses application of the anti-aging composition and a cosmetic.

Description

Technical Field

[0001] The present application relates to the technical field of daily chemical product production, and in particular to an anti-aging composition and its application, and cosmetics. Background Art

[0002] Under normal physiological conditions, human cells produce a small amount of free radicals during metabolism, such as superoxide anion free radicals (O2 - ), hydroxyl radical (·OH), etc. However, external factors such as ultraviolet radiation, air pollution, smoking, poor diet, etc. can significantly increase the production of free radicals. These free radicals have unpaired electrons and are very active in chemical properties. They will attack various biological molecules in the skin.

[0003] For example, free radicals attack lipid components in skin cells and trigger lipid peroxidation. The skin cell membrane is mainly composed of a lipid bilayer. Lipid peroxidation can destroy the structure and function of the cell membrane, resulting in reduced fluidity of the cell membrane and affecting the exchange of substances inside and outside the cell. At the same time, free radicals also attack proteins in the skin, such as collagen and elastic fibers. Collagen is an important component for maintaining skin firmness and elasticity, while elastic fibers help the skin stretch and retract. When free radicals attack collagen, they cause it to cross-link and denature, resulting in damage to the structure and function of collagen, causing the skin to lose elasticity and develop wrinkles.

[0004] In addition, free radicals can also damage the DNA in skin cells. DNA damage may cause cell mutations, affect the normal division and function of cells, and even lead to skin cell cancer in severe cases.

[0005] When too many free radicals are produced, exceeding the skin's own antioxidant defense system's ability to clear them, oxidative stress occurs. In this state, the redox balance in skin cells is broken, and a large amount of reactive oxygen species (ROS) accumulates, further exacerbating damage to skin cells. When the skin is in a state of oxidative stress for a long time, a series of aging symptoms will appear on the skin, such as dry, rough, sagging skin, and increased pigmentation.

[0006] Chinese patent application 202410733356.X discloses an antioxidant natural plant lip balm and a preparation method thereof, comprising the following raw materials in parts by weight: 55-67 parts of vegetable oil, 13-27 parts of vegetable wax, 6-8 parts of antioxidant, 2-5 parts of acid-base buffer, 4-6 parts of traditional Chinese medicine compound preparation, and 2-4 parts of natural plant flavoring agent; wherein the vegetable oil is composed of jojoba oil, meadowfoam seed oil, and coconut oil in a weight ratio of (8-12): (15-20): (12-18); the vegetable wax is composed of carnauba wax, candelilla wax, and olive wax in a weight ratio of 1: (3-6): (5-15); the antioxidant comprises tocopherol, pentaerythritol tetra(di-tert-butylhydroxyhydrocinnamate), and tea polyphenols; the raw materials for the traditional Chinese medicine compound preparation comprise golden vine, purslane, dandelion, cnidium monnieri, andrographis paniculata, hawthorn, and sophora flavescens;

[0007] The preparation method of the scheme can make the components in the lip balm evenly dispersed, give full play to their respective functions, and at the same time, the steps are simple, no complex production equipment is required, the cost is low, the manufacturing process is pollution-free, no toxic substances are discharged, and it is suitable for industrial production. The antioxidant natural plant lip balm of the scheme is applied to human skin to improve dry and cracked skin and make the skin ruddy and delicate.

[0008] Further observation of the instructions of the scheme shows that the antioxidants that mainly play an antioxidant role in the scheme include tocopherol, pentaerythritol tetra(di-tert-butylhydroxyhydrocinnamate) ester, and tea polyphenols.

[0009] Chinese patent application 202111005561.7 discloses a transparent microemulsion composition of plant seed oil that resists light damage, comprising: a plant seed oil complex, phospholipids and an Egyptian blue water lily extract, wherein the plant seed oil complex comprises 10-60 parts by weight of macadamia seeds, 30-40 parts by weight of tea seeds, 0-30 parts by weight of jojoba seeds, 0-50 parts by weight of walnut seeds, 0-30 parts by weight of peony seeds, 0-20 parts by weight of ginseng seeds, 20-40 parts by weight of cranberry seeds, 10-30 parts by weight of baobab seeds, 0-30 parts by weight of hazelnut seeds, 10-20 parts by weight of white pondweed seeds and 0-50 parts by weight of small-fruit coffee seeds;

[0010] The plant seed oil transparent microemulsion composition of the scheme has a natural, extensive and easily available raw material source, and the transparent microemulsion has good stability and high safety, and also has good light damage resistance.

[0011] Further observation of paragraph 38 of the instructions for the scheme shows that the scheme records that "Egyptian blue water lily extract is rich in 17 kinds of amino acids, and water lily protein is a high-quality protein. It also contains rich VC, flavonoids, and trace element zinc, which has a good conditioning, repairing, soothing and anti-allergic effect on cells and skin."

[0012] Meanwhile, the blue water lily extract in the scheme is a water-soluble blue water lily extract, and the scheme does not disclose the use of sunflower seed oil.

[0013] The problem to be solved in this scheme is: how to develop a composition that is different from the prior art and has good antioxidant capacity. Summary of the invention

[0014] The purpose of the present application is to provide an anti-aging composition, which is prepared by mixing a blue water lily extract with a vegetable oil containing sunflower seed oil. On the one hand, the anti-aging composition contains a blue water lily extract with good antioxidant capacity, and on the other hand, due to the rich linoleic acid and vitamin E in the sunflower seed oil, the antioxidant capacity of the composition is further enhanced.

[0015] To achieve the above-mentioned purpose, the present application discloses an anti-aging composition, comprising a blue water lily extract and a vegetable oil, wherein the mass ratio of the blue water lily extract to the vegetable oil is 0.5 to 2:4;

[0016] The vegetable oil is selected from at least one of sunflower seed oil, peony seed oil, olive oil, sweet almond oil, linseed oil and borage seed oil, and the vegetable oil at least contains sunflower seed oil.

[0017] Among them, the polyphenols and flavonoids in the blue water lily extract can directly react with free radicals and convert them into more stable molecules, thereby reducing the oxidative damage of free radicals to skin cells.

[0018] In addition, some components in blue water lily can chelate transition metal ions (such as iron and copper), which can catalyze peroxidation reactions in the body and generate more free radicals. By chelating these metal ions, blue water lily extract can indirectly reduce the generation of free radicals;

[0019] Blue water lily extract may enhance the antioxidant capacity of skin cells by regulating the activity of antioxidant enzymes (such as superoxide dismutase, catalase and glutathione peroxidase) in skin cells;

[0020] Blue water lily extract can also protect the integrity of cell membranes and prevent oxidative degradation of cell membrane lipids by inhibiting lipid peroxidation.

[0021] Sunflower seed oil is rich in fatty acids, especially linoleic acid, which makes it an excellent moisturizer. It can form a protective film on the surface of the skin, helping to lock in moisture and prevent skin from drying out.

[0022] Sunflower seed oil contains vitamin E, a powerful antioxidant that can help neutralize free radicals and reduce oxidative stress damage to the skin, thereby delaying skin aging;

[0023] At the same time, due to the rich linoleic acid content of sunflower seed oil, it helps to repair and maintain the skin's natural barrier function, which is especially important for sensitive skin or damaged skin, because a healthy skin barrier can better resist external irritation and pollutants;

[0024] Additionally, sunflower seed oil has some anti-inflammatory properties that can help soothe skin inflammation and irritation, reducing redness and discomfort;

[0025] Blue water lily extract may exert its antioxidant effect by regulating the antioxidant enzyme system in cells, thereby reducing the accumulation of reactive oxygen free radicals in cells. In addition to direct antioxidant effects, the nutrients in sunflower seed oil also have a certain regulatory effect on cell metabolism and function. It can provide cells with energy and nutrients, maintain the normal physiological functions of cells, and enable cells to better cope with oxidative stress. When the two work together, blue water lily extract regulates the activity of antioxidant enzymes while sunflower seed oil provides cells with good nutritional support, thereby enhancing the overall antioxidant capacity of cells and thus improving the antioxidant defense level of skin cells themselves, reducing the damage of free radicals to the skin and delaying skin aging.

[0026] The method for extracting the blue water lily extract comprises the following steps:

[0027] Step 1: Take a blue water lily flower and scratch its surface to obtain a blue water lily flower with a wound;

[0028] Step 2: placing the blue water lily flower with the wound in a culture medium to obtain a blue water lily callus;

[0029] Step 3: freeze-drying the blue water lily callus obtained in step 3, then mixing it with a 70% ethanol solution and extracting it in a water bath at a temperature of 55 to 65° C. for 2 to 3 hours, centrifuging, and taking the supernatant to obtain a blue water lily extract;

[0030] The culture medium is a liquid culture medium, and the liquid culture medium contains cytokinin and cell growth hormone;

[0031] The cytokinin is selected from at least one of trans-zeatin and cis-zeatin;

[0032] The cell growth hormone is selected from at least one of indoleacetic acid and naphthylacetic acid.

[0033] More preferably, the culture medium is an MS culture medium containing cytokinin and auxin, and the MS culture medium further contains 3% by mass of sucrose and 1.0% by mass of agar.

[0034] Preferably, the vegetable oil is a mixture of sunflower seed oil, peony seed oil and olive oil, and the mass ratio of sunflower seed oil, peony seed oil and olive oil is 4:0.01-0.3:0.01-0.3;

[0035] Furthermore, peony seed oil contains a large amount of unsaturated fatty acids, such as linolenic acid and linoleic acid. These unsaturated fatty acids have antioxidant potential. At the same time, peony seed oil also contains some natural antioxidants, such as vitamin E. Peony seed oil can also affect the antioxidant enzyme system in skin cells to a certain extent, thereby enhancing the antioxidant capacity of cells.

[0036] Olive oil is rich in phenolic compounds, such as hydroxytyrosol and oleuropein. These phenolic compounds have strong antioxidant activity, and some components in olive oil can also protect cell membranes. Cell membranes are mainly composed of lipid bilayers, which are easily attacked by free radicals and undergo peroxidation. The antioxidant components in olive oil can be embedded in the cell membrane to form a protective film. For example, oleuropein can react with free radicals on the surface of the cell membrane, preventing free radicals from combining with unsaturated fatty acids in the cell membrane, thereby avoiding lipid peroxidation of the cell membrane and maintaining the integrity and normal function of the cell membrane;

[0037] The antioxidant capacity of the composition is further enhanced by using the above two vegetable oils with antioxidant capacity together with the blue water lily extract and the sunflower seed oil.

[0038] Preferably, ginger root extract is further included, and the mass ratio of the ginger root extract, the blue water lily extract and the vegetable oil is 0.05-0.1:0.5-2:4.

[0039] Ginger root extract also contains a variety of gingerols with antioxidant capacity. At the same time, shogaol is also an active ingredient in ginger root extract. It also has antioxidant properties, and its antioxidant mechanism is similar to gingerol.

[0040] By adding ginger root extract, the antioxidant capacity of the composition is further improved.

[0041] In addition, the present application also discloses the use of the anti-aging composition as described above in preparing cosmetics.

[0042] In addition, the present application also discloses a cosmetic containing 0.5-10 wt % of the above anti-aging composition.

[0043] Preferably, it also includes skin conditioners, emollients, antioxidants, viscosity regulators, thickeners, solvents, and emulsifiers;

[0044] The skin conditioning agent is selected from at least one of Ganoderma lucidum extract, ginseng root extract, snow lotus extract, pentaerythritol tetra(di-tert-butyl hydroxyhydrocinnamate), bisabolol, green salicornia extract, ascorbyl tetraisopalmitate, tocopherol, maltodextrin, alpine edelweiss callus extract, coastal eryngium callus culture filtrate, sea fennel callus culture filtrate, apple fruit cell culture extract, grape fruit cell extract, desert rose leaf cell extract, lavender leaf cell extract, vitex negundo leaf cell extract, lotus leaf cell culture powder, myrrh leaf cell extract, jasmine leaf cell extract, acetyl hexapeptide-8, 1,2-hexanediol, glycerin, and damask rose flower water;

[0045] The emollient is selected from at least one of tocopherol, isohexadecane, isononyl isononanoate, and caprylic / capric triglyceride;

[0046] The antioxidant is tocopherol acetate;

[0047] The viscosity modifier is selected from at least one of stearalkonium hectorite and propylene carbonate;

[0048] The thickener is a hydrogenated (styrene / isoprene) copolymer;

[0049] The solvent is propylene glycol dicaprylate / dicaprate, hydrogenated polyisobutylene, and pure water;

[0050] The emulsifier is polyglyceryl-3 diisostearate.

[0051] Preferably, the mass ratio of the anti-aging composition, skin conditioner, emollient, antioxidant, viscosity regulator, thickener, emulsifier and solvent is 0.5-10: 0.5-1: 10-20: 0.2-1: 20-30: 3-8: 1-2: 45-60.

[0052] Preferably, the skin conditioner is a skin conditioner containing Ganoderma lucidum extract, ginseng root extract and snow lotus extract, and the mass ratio of Ganoderma lucidum extract, ginseng root extract and snow lotus extract in the skin conditioner is 6:1:1-3.

[0053] Preferably, the cosmetic is in the form of a patch, ointment, liquid, spray, gel, emulsion or cream.

[0054] The beneficial effects of this application are:

[0055] The polyphenols and flavonoids contained in the blue water lily extract of the present application can directly react with free radicals and convert them into relatively stable molecules, thereby reducing the oxidative damage of free radicals to skin cells;

[0056] In addition, some components in blue water lily can chelate transition metal ions (such as iron and copper), which can catalyze peroxidation reactions in the body and generate more free radicals. By chelating these metal ions, blue water lily extract can indirectly reduce the generation of free radicals;

[0057] Blue water lily extract may enhance the antioxidant capacity of skin cells by regulating the activity of antioxidant enzymes (such as superoxide dismutase, catalase and glutathione peroxidase) in skin cells;

[0058] Blue water lily extract can also protect the integrity of cell membranes and prevent oxidative degradation of cell membrane lipids by inhibiting lipid peroxidation.

[0059] Sunflower seed oil is rich in fatty acids, especially linoleic acid, which makes it an excellent moisturizer. It can form a protective film on the surface of the skin, helping to lock in moisture and prevent skin from drying out.

[0060] Sunflower seed oil contains vitamin E, a powerful antioxidant that can help neutralize free radicals and reduce oxidative stress damage to the skin, thereby delaying skin aging;

[0061] At the same time, due to the rich linoleic acid content of sunflower seed oil, it helps to repair and maintain the skin's natural barrier function, which is especially important for sensitive skin or damaged skin, because a healthy skin barrier can better resist external irritation and pollutants;

[0062] Additionally, sunflower seed oil has some anti-inflammatory properties that can help soothe skin inflammation and irritation, reducing redness and discomfort;

[0063] Blue water lily extract may exert its antioxidant effect by regulating the antioxidant enzyme system in cells, thereby reducing the accumulation of reactive oxygen free radicals in cells. In addition to direct antioxidant effects, the nutrients in sunflower seed oil also have a certain regulatory effect on cell metabolism and function. It can provide cells with energy and nutrients, maintain the normal physiological functions of cells, and enable cells to better cope with oxidative stress. When the two work together, blue water lily extract regulates the activity of antioxidant enzymes while sunflower seed oil provides cells with good nutritional support, thereby enhancing the overall antioxidant capacity of cells and thus improving the antioxidant defense level of skin cells themselves, reducing the damage of free radicals to the skin and delaying skin aging. DETAILED DESCRIPTION

[0064] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0065] Before presenting the embodiments and comparative examples, the raw materials involved in the embodiments and comparative examples are described as follows, as shown in Table 1:

[0066] Table 1

[0067]

[0068]

[0069] Extraction method of blue water lily extract:

[0070] Step 1: Take a blue water lily flower and scratch its surface to obtain a blue water lily flower with a wound;

[0071] Step 2: placing the blue water lily flower with wounds in a culture medium for 15 days to obtain blue water lily callus tissue;

[0072] Step 3: freeze-drying the blue water lily callus obtained in step 3, then mixing it with a 70% ethanol solution and extracting it in a water bath at a temperature of 60° C. for 3 hours, centrifuging, and taking the supernatant to obtain a blue water lily extract;

[0073] The culture medium is an MS culture medium containing 1.0% cytokinin by mass fraction and 1.0% cell growth factor by mass fraction, and the MS culture medium also contains 3.0% sucrose by mass fraction and 1.0% agar by mass fraction;

[0074] The cytokinin is trans-zeatin;

[0075] The cell growth hormone is indoleacetic acid.

[0076] It should be noted that, unless otherwise specified, the blue water lily extracts in the remaining examples and comparative examples were all prepared using the above method.

[0077] Example 1

[0078] A composition is obtained by mixing and dispersing a blue water lily extract and a vegetable oil in a mass ratio of 1:4;

[0079] The vegetable oil is sunflower seed oil.

[0080] Example 2

[0081] The method is substantially the same as Example 1, except that the mass ratio of the blue water lily extract to the sunflower seed oil is 0.5:4.

[0082] Example 3

[0083] The method is basically the same as Example 1, except that the mass ratio of the blue water lily extract to the vegetable oil is 2:4.

[0084] Example 4

[0085] The method is basically the same as Example 1, except that a mixture of sunflower seed oil, peony seed oil and olive oil having the same total mass as the sunflower seed oil in Example 1 is used to replace the sunflower seed oil, and the mass ratio of sunflower seed oil, peony seed oil and olive oil is 4:0.2:0.2.

[0086] Example 5

[0087] The method is substantially the same as Example 1, except that the blue water lily extract in Example 1 is replaced by a commercially available blue water lily extract.

[0088] Example 6

[0089] The method is basically the same as Example 1, except that the preparation method of the blue water lily extract is as follows:

[0090] Step 1: Take the root of the blue water lily and scratch its surface to obtain the root of the blue water lily with a wound;

[0091] Step 2: placing the wounded root of the blue water lily in a culture medium for 15 days to obtain a callus of the blue water lily;

[0092] Step 3: freeze-drying the blue water lily callus obtained in step 3, then mixing it with a 70% ethanol solution and extracting it in a water bath at a temperature of 60° C. for 3 hours, centrifuging, and taking the supernatant to obtain a blue water lily extract;

[0093] The culture medium is an MS culture medium containing 1.0% cytokinin by mass fraction and 1.0% cell growth factor by mass fraction, and the MS culture medium also contains 3.0% sucrose by mass fraction and 1.0% agar by mass fraction;

[0094] The cytokinin is trans-zeatin;

[0095] The cell growth hormone is indoleacetic acid.

[0096] Example 7

[0097] The method is basically the same as Example 1, except that the preparation method of the blue water lily extract is as follows:

[0098] Step 1: Take the stem of the blue water lily and scratch its surface to obtain the stem of the blue water lily with a wound;

[0099] Step 2: placing the wounded stem of the blue water lily in a culture medium for 15 days to obtain a callus of the blue water lily;

[0100] Step 3: freeze-drying the blue water lily callus obtained in step 3, then mixing it with a 70% ethanol solution and extracting it in a water bath at a temperature of 60° C. for 3 hours, centrifuging, and taking the supernatant to obtain a blue water lily extract;

[0101] The culture medium is an MS culture medium containing 1.0% cytokinin by mass fraction and 1.0% cell growth factor by mass fraction, and the MS culture medium also contains 3.0% sucrose by mass fraction and 1.0% agar by mass fraction;

[0102] The cytokinin is trans-zeatin;

[0103] The cell growth hormone is indoleacetic acid.

[0104] Example 8

[0105] The method is basically the same as Example 1, except that cis-zeatin is used to replace trans-zeatin.

[0106] Example 9

[0107] The method is substantially the same as Example 1, except that naphthylacetic acid is used to replace indoleacetic acid.

[0108] Comparative Example 1

[0109] The method is basically the same as Example 1, except that a mixture of peony seed oil and olive oil having the same total mass as the sunflower seed oil in Example 1 is used to replace the sunflower seed oil, and the mass ratio of peony seed oil to olive oil is 0.2:0.2.

[0110] Comparative Example 2

[0111] Basically the same as Example 1, except that the sunflower seed oil is replaced with macadamia seed oil having the same total mass as the sunflower seed oil in Example 1.

[0112] Comparative Example 3

[0113] The method is basically the same as Example 1, except that tea seed oil having the same total mass as the sunflower seed oil in Example 1 is used to replace the sunflower seed oil.

[0114] Comparative Example 4

[0115] The method is substantially the same as Example 1, except that cranberry seed oil having the same total mass as the sunflower seed oil in Example 1 is used to replace the sunflower seed oil.

[0116] Comparative Example 5

[0117] The method is basically the same as Example 1, except that white water lily flowers are used to replace blue water lily flowers to finally prepare white water lily flower extract.

[0118] Performance Test:

[0119] 1. Non-enzymatic glycosylation test:

[0120] 1.1 Definition of glycosylation and non-enzymatic glycosylation: Glycosylation is the process of attaching sugars to proteins or lipids under the control of enzymes, which occurs in the endoplasmic reticulum. Sugars are transferred to proteins under the action of glycosyltransferases, forming glycosidic bonds with amino acid residues on proteins. Proteins undergo glycosylation to form glycoproteins. Glycosylation is an important modification of proteins and has the function of regulating protein functions.

[0121] Non-enzymatic glycosylation is a series of complex non-enzymatic reactions. Proteins and glucose react in the body to form early glycosylation products such as Schiff bases and Amadori products, which then undergo oxidation, rearrangement, cross-linking and other processes to form irreversible non-enzymatic glycosylation end products. Non-enzymatic glycosylation reactions are widely and slowly carried out in organisms, which can lead to reduced protein function and aging, and then cause aging and pathology of body tissues.

[0122] 1.2 Test method:

[0123] Preparation of PBS: Prepare PBS with a pH of 7.3-7.4, and add 0.02% (mass percentage) of sodium azide thereto.

[0124] BSA (bovine serum albumin)-fructose reaction solution: 20 mL of BSA solution (20 mg / mL) with PBS as solvent was mixed with 20 mL of fructose solution (0.5 mol / L).

[0125] Preparation of the sample to be tested: First, the composition prepared in the embodiment and the comparative example is diluted with PBS until the mass fraction of the composition in the sample to be tested is 1%, thereby obtaining the sample to be tested.

[0126] Inhibition group: Each sample to be tested was mixed with the BSA-fructose reaction solution to serve as the inhibition group.

[0127] Negative control group: PBS was selected instead of the sample to be tested and mixed with the BSA-fructose reaction solution as the negative control group. The negative control group reflects the situation that there is no inhibitory effect on the non-enzymatic glycosylation reaction in the system.

[0128] Positive control group: Aminoguanidine was selected as a positive control and mixed with the BSA-fructose reaction solution as a positive control group.

[0129] Blank group: Each sample to be tested was mixed with PBS separately without adding reaction solution, which served as the blank group.

[0130] 1.3 Sample testing

[0131] (1) Prepare different test groups according to the ratios given in Table 2 and incubate in a 37°C constant temperature incubator away from light;

[0132] (2) On the 5th day, the fluorescence intensity was measured (excitation wavelength: 370 nm, emission wavelength: 440 nm).

[0133] Table 2

[0134]

[0135] Note: In Table 2, “+” represents addition, and “-” represents no addition.

[0136] 1.6 Test results

[0137] The calculation formula of inhibition rate (IR) is shown in formula 1:

[0138]

[0139] Among them, RFU stands for Relative Fluorescence Unit, relative fluorescence unit;

[0140] The final test results are shown in Table 3:

[0141] Table 3

[0142] Group Inhibition rate (%) Group Inhibition rate (%) Example 1 23.6％ Example 9 23.0％ Example 2 22.8％ Comparative Example 1 15.2％ Example 3 23.1％ Comparative Example 2 13.5％ Example 4 26.3％ Comparative Example 3 12.9％ Example 5 21.8％ Comparative Example 4 13.2％ Example 6 19.7％ Comparative Example 5 17.4％ Example 7 20.3％ Positive control group 33.5％ Example 8 22.4％

[0143] 2. Antioxidant (MDA inhibition) test:

[0144] 2.1 Test Method

[0145] MDA was determined using a thiobarbituric acid (TBA) kit.

[0146] 2.2 Grouping, modeling and drug administration

[0147] The grouping, modeling and administration process is as follows: 170 guinea pigs are randomly divided into a blank group, a model group, a positive control group (using 1% hydroquinone cream) and a sample group (the sample group corresponds to the composition prepared in the embodiment and the comparative example), a total of 17 groups, each group has 10. And 3cm×3cm skin is taken from the back of each group of guinea pigs for depilation once a week. Except for the blank group, the other groups are irradiated with medium-wave ultraviolet rays with a wavelength of 320nm once a day, each irradiation time is 60min, the distance between the animal and the light source is 10cm, and the irradiation is continuous for 4 weeks. The skin changes at the depilated parts of the back of the guinea pigs in each group are continuously observed. The modeling is successful if obvious black deposition is visible to the naked eye. After the modeling is successful, the sample group (the composition prepared in the embodiment and the comparative example is diluted with pure water to a mass fraction of 1.0% aqueous solution) and the positive control group (1.0% hydroquinone cream) are applied to the skin lesions of each guinea pig, once a day, and massage for 3min each time. After continuous use for 4 weeks, the changes in the skin of the guinea pigs in each group were observed. The model group and the blank group were not given any drug intervention.

[0148] 2.3 Sampling and testing indicators

[0149] The sampling and testing indicators are: after 4 weeks of continuous use, all animals were depilated on the back and killed by breaking the neck, and skin tissue was quickly taken after depilation. The results of MDA detection in guinea pig skin are: compared with the blank group, the MDA content increased. Compared with the model group, the MDA content in the skin of the guinea pig experimental area in the positive control group and the sample group decreased.

[0150] The specific test results are shown in Table 4:

[0151] Table 4

[0152]

[0153] Application Example 1-14

[0154] A cosmetic is prepared by respectively corresponding to the compositions prepared by Examples 1-9 and Comparative Examples 1-5;

[0155] The specific preparation method is:

[0156] The composition prepared in the embodiment or comparative example is mixed with a skin conditioner, an emollient, an antioxidant, a viscosity regulator, a thickener, an emulsifier, and a solvent in a mass content ratio of 1:1:15:0.5:25:6:1.5:50, and dispersed to obtain a cosmetic;

[0157] The skin conditioning agent is specifically Ganoderma lucidum extract;

[0158] The emollient is specifically a mixture of tocopherol and isohexadecane, and the mass ratio of tocopherol to isohexadecane is 0.1:15;

[0159] The antioxidant is tocopheryl acetate;

[0160] The viscosity regulator is stearalkonium hectorite;

[0161] The thickener is hydrogenated (styrene / isoprene) copolymer;

[0162] The solvent is water and propylene glycol dicaprylate / dicaprate, and the mass ratio between the two is 1.8:1.

[0163] The emulsifier is polyglyceryl-3 diisostearate.

[0164] Application Example 15

[0165] The method is basically the same as Application Example 1, except that the skin conditioning agent is a mixture of ginseng root extract and snow lotus extract, and the mass ratio of the ginseng root extract to the snow lotus extract is 1:1.

[0166] Application Example 16

[0167] It is basically the same as Application Example 1, except that the skin conditioner is a mixture of Ganoderma lucidum extract, ginseng root extract, and snow lotus extract, and the mass ratio of Ganoderma lucidum extract, ginseng root extract, and snow lotus extract is 6:1:1.

[0168] Application Example 17

[0169] It is basically the same as Application Example 1, except that the emollient is tocopherol.

[0170] Application Example 18

[0171] It is basically the same as Application Example 1, except that the emollient is isohexadecane.

[0172] Application Example 19

[0173] It is basically the same as Application Example 1, except that the cosmetic lacks an antioxidant (tocopherol acetate).

[0174] Application Example 20

[0175] It is basically the same as Application Example 1, except that the cosmetic lacks the skin conditioning agent (Ganoderma lucidum extract).

[0176] Application Example 21

[0177] It is basically the same as Application Example 1, except that the cosmetic lacks an emollient (a mixture of tocopherol and isohexadecane).

[0178] Application Example 22

[0179] It is basically the same as Application Example 1, except that the composition prepared in Example 1 is not added.

[0180] Performance Test:

[0181] 3. Non-enzymatic glycosylation test (for application examples):

[0182] 3.1 Test method:

[0183] Preparation of PBS: Prepare PBS with a pH of 7.3-7.4, and add 0.02% (mass percentage) of sodium azide thereto.

[0184] BSA (bovine serum albumin)-fructose reaction solution: 20 mL of BSA solution (20 mg / mL) with PBS as solvent was mixed with 20 mL of fructose solution (0.5 mol / L).

[0185] Prepare the samples to be tested: Weigh the finished cosmetics obtained in each application example to obtain the samples to be tested.

[0186] Inhibition group: Each sample to be tested was mixed with the BSA-fructose reaction solution to serve as the inhibition group.

[0187] Negative control group: PBS was selected instead of the sample to be tested and mixed with the BSA-fructose reaction solution as the negative control group. The negative control group reflects the situation that there is no inhibitory effect on the non-enzymatic glycosylation reaction in the system.

[0188] Positive control group: Aminoguanidine was selected as a positive control and mixed with the BSA-fructose reaction solution as a positive control group.

[0189] Blank group: Each sample to be tested was mixed with PBS separately without adding reaction solution, which served as the blank group.

[0190] 3.2 Sample testing

[0191] (1) Prepare different test groups according to the ratio given in Table 5 and incubate in a 37°C constant temperature incubator away from light;

[0192] (2) On the 5th day, the fluorescence intensity was measured (excitation wavelength: 370 nm, emission wavelength: 440 nm).

[0193] Table 5

[0194] Trial Groups Samples to be tested Positive PBS Reaction solution Sample set + － － + Negative control group － － + + Positive control group － + － + Blank control group + － + －

[0195] Note: In Table 2, “+” represents addition, and “-” represents no addition.

[0196] 1.3 Test results

[0197] The calculation formula of inhibition rate (IR) is shown in formula 2:

[0198]

[0199] Among them, RFU is Relative Fluorescence Unit, relative fluorescence unit; the final test results are shown in Table 6:

[0200] Table 6

[0201] Group Inhibition rate (%) Group Inhibition rate (%) Application Example 1 34.8％ Application Example 13 17.8％ Application Example 2 33.2％ Application Example 14 21.9％ Application Example 3 33.5％ Application Example 15 32.7％ Application Example 4 37.4％ Application Example 16 36.4％ Application Example 5 31.0％ Application Example 17 32.3％ Application Example 6 29.4％ Application Example 18 31.7％ Application Example 7 29.7％ Application Example 19 32.2％ Application Example 8 31.9％ Application Example 20 33.0％ Application Example 9 32.6％ Application Example 21 32.6％ Application Example 10 20.4％ Application Example 22 6.9％ Application Example 11 18.3％ Positive control group 33.8％ Application Example 12 18.0％

[0202] 4. Antioxidant (MDA inhibition) test (for application examples):

[0203] 4.1 Test Method

[0204] MDA was determined using a thiobarbituric acid (TBA) kit.

[0205] 4.2 Grouping, modeling and drug administration

[0206] The grouping, modeling and drug administration process is as follows: 250 guinea pigs were randomly divided into 25 groups, including a blank group, a model group, a positive control group (using 1% hydroquinone cream) and a sample group (the finished cosmetic product prepared in the application example), with 10 in each group. A 3cm×3cm skin was taken from the back of each group of guinea pigs for hair removal once a week. Except for the blank group, the other groups were irradiated with medium-wave ultraviolet rays with a wavelength of 320nm once a day, each irradiation time was 60min, and the distance between the animal and the light source was 10cm. The irradiation lasted for 4 weeks, and the skin changes at the hair removal site on the back of the guinea pigs in each group were continuously observed. The model was successful when obvious black deposition was visible to the naked eye. After the model was successfully established, the sample group and the positive control group (1% hydroquinone cream) were applied to the skin lesions of each guinea pig, once a day, and massaged for 3 minutes each time. After 4 weeks of continuous use, the changes in the skin at the hair removal site of each group of guinea pigs were observed. No drug intervention was given to the model group and the blank group.

[0207] 4.3 Sampling and testing indicators

[0208] The sampling and testing indicators are: after 4 weeks of continuous use, all animals were depilated on the back and killed by breaking the neck, and the skin tissue was quickly taken after depilation. The results of the MDA test in the guinea pig skin showed that compared with the blank group, the MDA content was significantly increased. Compared with the model group, the MDA content in the skin of the guinea pig experimental area in the positive control group and the sample group was significantly reduced.

[0209] The specific test results are shown in Table 7:

[0210] Table 7

[0211]

[0212]

[0213] Result analysis:

[0214] 1. It can be seen from Table 3 that when the mass ratio between the blue water lily extract and the vegetable oil is slightly adjusted in Examples 1-3, the inhibition rate of Examples 1-3 fluctuates, but the amplitude is very small;

[0215] 2. Further observation of Example 4 shows that when sunflower oil is replaced with a variety of vegetable oils, the inhibition ability of Example 4 is improved, indicating that the compounding of vegetable oils can improve the inhibition rate of the composition on non-enzymatic glycosylation;

[0216] 3. By observing Examples 5-7, it can be seen that after replacing the blue water lily extract of Example 1 with the commercially available blue water lily extract or the blue water lily extract extracted from other locations, the inhibitory ability of Examples 5-7 on non-enzymatic glycosylation is weakened, proving that the extraction method and extraction location of Example 1 are more advantageous;

[0217] 4. It can be seen from Examples 8-9 that when either trans-zeatin or indoleacetic acid is replaced, the non-enzymatic glycosylation inhibition ability of the composition is reduced. It can be seen that the active ingredient content of the blue water lily extract obtained by culturing with the above two cytokinins and cell growth hormones is higher;

[0218] 5. It can be seen from comparative examples 1-4 that after other vegetable oils were used to replace sunflower seed oil, the non-enzymatic glycosylation inhibition ability of the composition declined. It can be seen that the sunflower seed oil in this solution cannot be replaced;

[0219] Furthermore, after replacing the blue water lily extract with the white water lily extract, its inhibitory ability declined significantly, indicating that the blue water lily extract in this solution cannot be replaced;

[0220] 6. Further observation of Table 4 shows that after sunflower oil is replaced by various vegetable oils in Example 4, the MDA value of Example 4 decreases compared with Examples 1-3, indicating that the compounding of vegetable oils can simultaneously enhance the MDA inhibition ability of the composition;

[0221] At the same time, the MDA values ​​of Comparative Examples 1-5 are higher than those of Example 1. It can be further seen that in terms of antioxidant properties, the sunflower seed oil and blue water lily extract of this solution cannot be replaced;

[0222] 7. From Table 6, it can be seen that the inhibitory ability of Application Examples 1-14 on non-enzymatic glycosylation is improved compared with that of Examples 1-9 and Comparative Examples 1-5. It can be seen that other ingredients in cosmetics also have a certain ability to inhibit non-enzymatic glycosylation;

[0223] However, it can be seen from Application Example 22 that the non-enzymatic glycosylation inhibition rate of other ingredients is 6.9%; while the non-enzymatic glycosylation inhibition rate of the composition measured in Example 1 is 23.6%; the sum of the two is about 30.5% and is weaker than that of Application Example 1. It can be seen that in terms of non-enzymatic glycosylation inhibition, the composition has a synergistic effect with other components in the cosmetics;

[0224] 8. Further observation of Application Examples 15-16 shows that after further optimization of the skin conditioning agent in Application Example 16, the inhibition rate of Application Example 16 is improved. It can be seen that the synergistic effect between the three skin conditioning agents or between the skin conditioning agent and the composition is further amplified;

[0225] It can be seen from Application Examples 17-18 that the presence of tocopherol and isohexadecane in the emollient enhances the non-enzymatic glycation inhibition ability of the cosmetics, while when the cosmetics lack any one of the antioxidants, skin conditioners, and emollients, the non-enzymatic glycation inhibition ability of Application Examples 19-21 is weakened compared with Example 1;

[0226] 9. Further observation of Table 7 shows that the average MDA values ​​of Application Examples 1-14 in Table 7 are further suppressed relative to those of Examples 1-9 and Comparative Examples 1-5. This shows that the composition and other ingredients in the cosmetics have a common effect on the antioxidant capacity.

Claims

1. An anti-aging composition, characterized in that: The invention comprises a blue water lily extract and vegetable oil, wherein the mass ratio of the blue water lily extract to the vegetable oil is 0.5 to 2:4; The vegetable oil is selected from at least one of sunflower seed oil, peony seed oil, olive oil, sweet almond oil, linseed oil and borage seed oil, and the vegetable oil at least contains sunflower seed oil.

2. The anti-aging composition according to claim 1, characterized in that: The method for extracting the blue water lily extract comprises the following steps: Step 1: Take a blue water lily flower and scratch its surface to obtain a blue water lily flower with a wound; Step 2: placing the blue water lily flower with the wound in a culture medium to obtain a blue water lily callus; Step 3: freeze-drying the blue water lily callus obtained in step 3, then mixing it with a 70% ethanol solution and extracting it in a water bath at a temperature of 55 to 65° C. for 2 to 3 hours, centrifuging, and taking the supernatant to obtain a blue water lily extract; The culture medium is a culture medium containing cytokinin and cell growth hormone; The cytokinin is selected from at least one of trans-zeatin and cis-zeatin; The cell growth hormone is selected from at least one of indoleacetic acid and naphthylacetic acid.

3. The anti-aging composition according to claim 1, characterized in that: The vegetable oil is a mixture of sunflower seed oil, peony seed oil and olive oil, and the mass ratio of sunflower seed oil, peony seed oil and olive oil is 4:0.01-0.3:0.01-0.

3.

4. The anti-aging composition according to claim 1, characterized in that: It also includes ginger root extract, wherein the mass ratio of the ginger root extract, the blue water lily extract and the vegetable oil is 0.05-0.1:0.5-2:

4.

5. Use of the anti-aging composition according to any one of claims 1 to 4 in preparing cosmetics.

6. A cosmetic, characterized in that: Contains 0.5-10wt% of the anti-aging composition according to any one of claims 1-4.

7. The cosmetic according to claim 6, characterized in that: It also includes skin conditioners, emollients, antioxidants, viscosity regulators, thickeners, solvents, and emulsifiers; The skin conditioning agent is selected from at least one of Ganoderma lucidum extract, ginseng root extract, snow lotus extract, pentaerythritol tetra(di-tert-butyl hydroxyhydrocinnamate), bisabolol, green salicornia extract, ascorbyl tetraisopalmitate, tocopherol, maltodextrin, alpine edelweiss callus extract, coastal eryngium callus culture filtrate, sea fennel callus culture filtrate, apple fruit cell culture extract, grape fruit cell extract, desert rose leaf cell extract, lavender leaf cell extract, vitex negundo leaf cell extract, lotus leaf cell culture powder, myrrh leaf cell extract, jasmine leaf cell extract, acetyl hexapeptide-8, 1,2-hexanediol, glycerin, and damask rose flower water; The emollient is selected from at least one of tocopherol, isohexadecane, isononyl isononanoate, and caprylic / capric triglyceride; The antioxidant is tocopherol acetate; The viscosity modifier is selected from at least one of stearalkonium hectorite and propylene carbonate; The thickener is a hydrogenated (styrene / isoprene) copolymer; The solvent is propylene glycol dicaprylate / dicaprate, hydrogenated polyisobutylene, and pure water; The emulsifier is polyglyceryl-3 diisostearate.

8. The cosmetic according to claim 7, characterized in that: The mass ratio of the anti-aging composition, skin conditioner, emollient, antioxidant, viscosity regulator, thickener, emulsifier and solvent is 0.5-10: 0.5-1: 10-20: 0.2-1: 20-30: 3-8: 1-2: 45-60.

9. The cosmetic according to claim 7, characterized in that: The skin conditioner contains Ganoderma lucidum extract, ginseng root extract and snow lotus extract. The mass ratio of Ganoderma lucidum extract, ginseng root extract and snow lotus extract in the skin conditioner is 6:1:1-3.

10. The cosmetic according to claim 6, characterized in that: The dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion or cream.

Images