Polypeptide dressing with whitening effect and preparation method thereof

Abstract

The invention is suitable for the technical field of polypeptide dressings, and provides a polypeptide dressing with a whitening effect and a preparation method of the polypeptide dressing. The polypeptide dressing is prepared from the following whitening components in percentage by weight: 0.2%-0.6% of a caulis schisandrae chinensis extract, 0.1%-1% of glutathione, 0.1%-0.5% of yeast polypeptide, 0.1%-0.5% of oligopeptide-1, 1%-3% of nicotinamide, 0.1%-1% of silk fibroin, 0.01%-0.1% of recombinant human collagen and the balance of water. Preferably, the polypeptide dressing further comprises a humectant, a preservative and a thickening agent. Preferably, the thickening agent is a carbomer homopolymer, and the mass percent of the thickening agent is 0.15%-0.2%; preferably, the humectant is prepared from the following components in percentage by mass: 0.05 to 0.2 percent of sodium hyaluronate, 1 to 3 percent of mussel mucin and 3 to 5 percent of glycerol. The polypeptide dressing prepared by the preparation method disclosed by the invention can be used for improving the darkness of the skin and reducing the generation of melanin by inhibiting the activity of tyrosinase, so that the effects of whitening and moisturizing are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide dressings, in particular to a polypeptide dressing with whitening effect and a preparation method thereof. Background Art

[0002] Whitening is a skin care effect pursued by most women and has always been a hot topic in the cosmetics industry. The color-forming substances in human skin are melanin, hemoglobin and carotenoids. The content and distribution of melanin in the skin play a decisive role in the color of human skin. Therefore, the relevant research on the mechanism of melanin production has always been a research hotspot.

[0003] The formation of melanin is closely related to tyrosinase. Studies have shown that tyrosinase is the main rate-limiting enzyme in the formation of melanin, and its activity will directly affect the amount of melanin produced. This is also the reason why the whitening effect of skin whitening agents is often measured by testing their inhibitory ability during the development of skin whitening agents.

[0004] Most of the existing whitening dressings have poor effects and cannot meet people's needs. In this regard, the present invention provides a polypeptide dressing with whitening effect and a preparation method thereof. Summary of the invention

[0005] The object of the present invention is to provide a polypeptide dressing with whitening effect and a preparation method thereof, so as to solve the problems in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: In a first aspect, the present invention provides a polypeptide dressing with whitening effect. The whitening components and contents of the polypeptide dressing are as follows, in terms of mass percentage:

[0007] 0.2%-0.6% of Glehnia littoralis extract, 0.1%-1% of glutathione, 0.1%-0.5% of yeast polypeptide, 0.1%-0.5% of oligopeptide-1, 1%-3% of niacinamide, 0.1%-1% of silk fibroin, 0.01%-0.1% of recombinant human collagen, and the balance of water.

[0008] Preferably, the polypeptide dressing further comprises a moisturizer, a preservative, and a thickener.

[0009] Preferably, the thickener is carbomer homopolymer, and the mass percentage of the thickener is 0.15%-0.2%.

[0010] Preferably, the ingredients and mass percentages of the moisturizing agent are: sodium hyaluronate 0.05%-0.2%, mussel mucin 1%-3%, and glycerol 3%-5%.

[0011] Preferably, the preservative is methylparaben, and the mass percentage of the preservative is 0.15%-0.2%.

[0012] The second aspect of the present invention provides a method for preparing the polypeptide dressing according to the first aspect of the present invention, comprising the following steps:

[0013] 1) Preparation of solution 1: weigh a certain amount of purified water and pour it into a water pot, add the extract of Glehnia littoralis, glutathione, yeast polypeptide, oligopeptide-1, niacinamide, silk fibroin, recombinant human collagen, sodium hyaluronate, mussel mucin and triethanolamine, stir and dissolve for later use;

[0014] 2) Preparation of solution 2: weigh a certain amount of purified water and pour it into the main pot, add glycerin, methylparaben and carbomer homopolymer, heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes;

[0015] 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.

[0016] The present invention has at least the following beneficial effects:

[0017] The present invention provides a polypeptide dressing with whitening effect and a preparation method thereof. The prepared polypeptide dressing can improve dull skin and reduce the production of melanin by inhibiting the activity of tyrosinase, thereby playing a whitening and moisturizing role. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The present invention provides a polypeptide dressing with whitening effect. The whitening components and contents of the polypeptide dressing are as follows, in terms of mass percentage:

[0020] 0.2%-0.6% of Glehnia littoralis extract, 0.1%-1% of glutathione, 0.1%-0.5% of yeast polypeptide, 0.1%-0.5% of oligopeptide-1, 1%-3% of niacinamide, 0.1%-1% of silk fibroin, 0.01%-0.1% of recombinant human collagen, and the balance of water.

[0021] The polypeptide dressing also includes a moisturizer, a preservative, and a thickener. The thickener is a carbomer homopolymer, and the mass percentage of the thickener is 0.15%-0.2%; the composition and mass percentage of the moisturizer are: sodium hyaluronate 0.05%-0.2%, mussel mucin 1%-3%, glycerol 3%-5%; the preservative is methylparaben, and the mass percentage of the preservative is 0.15%-0.2%.

[0022] The present invention also provides a method for preparing a polypeptide dressing with whitening effect, comprising the following steps:

[0023] 1) Preparation of solution 1: weigh a certain amount of purified water and pour it into a water pot, add the extract of Glehnia littoralis, glutathione, yeast polypeptide, oligopeptide-1, niacinamide, silk fibroin, recombinant human collagen, sodium hyaluronate, mussel mucin and triethanolamine, stir and dissolve for later use;

[0024] 2) Preparation of solution 2: weigh a certain amount of purified water and pour it into the main pot, add glycerin, methylparaben and carbomer homopolymer, heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes;

[0025] 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.

[0026] Based on the above technical solution, the present invention provides the following partial embodiments:

[0027] Example 1

[0028] Example 1 discloses a polypeptide dressing with whitening effect, comprising the following raw materials in percentage by mass: 0.2% of Glehnia littoralis extract, 0.3% of glutathione, 0.1% of yeast polypeptide, 0.1% of oligopeptide-1, 1% of niacinamide, 0.1% of silk fibroin, 0.01% of recombinant human collagen, 0.05% of sodium hyaluronate, 1% of mussel mucin, 3% of glycerol, 0.15% of methylparaben, 0.15% of carbomer homopolymer, 0.15% of triethanolamine and the remainder of water.

[0029] Preparation method:

[0030] 1) Preparation of solution 1: Weigh a certain amount of purified water (40% of the total purified water) and pour it into a water pot, add 0.2% of Glehnia littoralis extract, 0.3% of glutathione, 0.1% of yeast polypeptide, 0.1% of oligopeptide-1, 1% of niacinamide, 0.1% of silk fibroin, 0.01% of recombinant human collagen, 0.05% of sodium hyaluronate, 1% of mussel mucin and 0.15% of triethanolamine, stir and dissolve for later use.

[0031] 2) Preparation of solution 2: Weigh a certain amount of purified water (60% of the total purified water) and pour it into the main pot, add 3% glycerol, 0.15% methylparaben and 0.15% carbomer homopolymer (type C), heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes.

[0032] 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.

[0033] Example 2

[0034] Example 2 discloses a polypeptide dressing with whitening effect, comprising the following raw materials in percentage by mass: 0.4% of Glehnia littoralis extract, 0.4% of glutathione, 0.3% of yeast polypeptide, 0.3% of oligopeptide-1, 2% of niacinamide, 0.3% of silk fibroin, 0.05% of recombinant human collagen, 0.1% of sodium hyaluronate, 2% of mussel mucin, 4% of glycerol, 0.18% of methylparaben, 0.18% of carbomer homopolymer, 0.18% of triethanolamine and the remainder of water.

[0035] Preparation method:

[0036] 1) Preparation of solution 1: Weigh a certain amount of purified water (40% of the total purified water) and pour it into a water pot, add 0.4% of Glehnia littoralis extract, 0.4% of glutathione, 0.3% of yeast polypeptide, 0.3% of oligopeptide-1, 2% of niacinamide, 0.3% of silk fibroin, 0.05% of recombinant human collagen, 0.1% of sodium hyaluronate, 2% of mussel mucin and 0.18% of triethanolamine, stir and dissolve for later use.

[0037] 2) Preparation of solution 2: Weigh a certain amount of purified water (60% of the total purified water) and pour it into the main pot, add 4% glycerol, 0.18% methylparaben and 0.18% carbomer homopolymer (type C), heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes.

[0038] 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.

[0039] Example 3

[0040] Example 3 discloses a polypeptide dressing with whitening effect, comprising the following raw materials in percentage by mass: 0.6% of Glehnia littoralis extract, 0.6% of glutathione, 0.5% of yeast polypeptide, 0.5% of oligopeptide-1, 3% of niacinamide, 1% of silk fibroin, 0.1% of recombinant human collagen, 0.2% of sodium hyaluronate, 3% of mussel mucin, 5% of glycerol, 0.2% of methylparaben, 0.2% of carbomer homopolymer, 0.2% of triethanolamine and the remainder of water.

[0041] Preparation method:

[0042] 1) Preparation of solution 1: Weigh a certain amount of purified water (40% of the total purified water) and pour it into a water pot, add 0.6% of Glehnia littoralis extract, 0.6% of glutathione, 0.5% of yeast polypeptide, 0.5% of oligopeptide-1, 3% of niacinamide, 1% of silk fibroin, 0.1% of recombinant human collagen, 0.2% of sodium hyaluronate, 3% of mussel mucin and 0.2% of triethanolamine, stir and dissolve for later use.

[0043] 2) Preparation of solution 2: Weigh a certain amount of purified water (60% of the total purified water) and pour it into the main pot, add 5% glycerol, 0.2% methylparaben and 0.2% carbomer homopolymer (type C), heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes.

[0044] 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.

[0045] Comparative Example 1

[0046] Comparative Example 1 is obtained by removing the Glehnia littoralis extract in Example 1, and the composition includes 0.3% glutathione, 0.1% yeast polypeptide, 0.1% oligopeptide-1, 1% niacinamide, 0.1% silk fibroin, 0.01% recombinant human collagen, 0.05% sodium hyaluronate, 1% mussel mucin, 3% glycerol, 0.15% methylparaben, 0.15% carbomer homopolymer, 0.15% triethanolamine and the remainder water.

[0047] The preparation method is the same as Example 1.

[0048] Comparative Example 2

[0049] Comparative Example 2 is obtained by removing the glutathione in Example 2, and is composed of 0.4% of Glehnia littoralis extract, 0.3% of yeast polypeptide, 0.3% of oligopeptide-1, 2% of niacinamide, 0.3% of silk fibroin, 0.05% of recombinant human collagen, 0.1% of sodium hyaluronate, 2% of mussel mucin, 4% of glycerol, 0.18% of methylparaben, 0.18% of carbomer homopolymer, 0.18% of triethanolamine and the remainder of water.

[0050] The preparation method is the same as Example 2.

[0051] Comparative Example 3

[0052] Comparative Example 3 is obtained by removing the silk fibroin in Example 3, and its composition is: 0.6% of Glehnia littoralis extract, 0.6% of glutathione, 0.5% of yeast polypeptide, 0.5% of oligopeptide-1, 3% of niacinamide, 0.1% of recombinant human collagen, 0.2% of sodium hyaluronate, 3% of mussel mucin, 5% of glycerol, 0.2% of methylparaben, 0.2% of carbomer homopolymer, 0.2% of triethanolamine and the remainder of water.

[0053] The preparation method is the same as Example 3.

[0054] In the present invention:

[0055] Glutathione is a short peptide composed of three amino acids: glutamic acid, cysteine ​​and glycine. It can bind to the copper ions in tyrosinase, inhibit its activity, and remove oxygen free radicals in the body, thereby inhibiting the proliferation of melanocytes and slowing down skin pigmentation. Glutathione can also regulate the metabolism of melanin intermediates to generate other products, thereby blocking the synthesis of melanin, making the skin appear lighter brown melanin, and thus brightening the skin tone.

[0056] Oligopeptide-1 is an important active protein polypeptide substance in the human body. It can promote the growth of basal tissue, promote the overall metabolic rate of epidermal and dermal cells, reduce the content of melanin and colored cells in skin cells, reduce skin pigmentation, accelerate skin barrier repair, and improve acne and red blood streaks.

[0057] Nicotinamide, also known as nicotinamide, is an amide compound of nicotinic acid. It is a white crystalline powder, odorless or almost odorless, and slightly hygroscopic. Studies have shown that niacinamide has the function of inhibiting the transfer of melanin from melanocytes to keratinocytes. At the same time, niacinamide can accelerate the shedding of epidermal cells containing melanin by promoting skin metabolism. Although niacinamide cannot fundamentally control the production of melanin, it can intervene in its transport channel, making it difficult for it to reach the epidermis, and at the same time accelerate the shedding of melanin that has already formed, thereby reducing excessive pigmentation.

[0058] Tianxiangteng is a plant of the Fabaceae family and the genus Albizia. It is a climbing shrub or vine, about 20 meters long; the young branches are slightly pubescent, and there is often a short, thick thorn that bends downward under the petiole. The stipules are small and fall off. It grows by the water or in wet places. It is distributed in Guangdong, Guangxi and other places. Studies have shown that Tianxiangteng extract can remove free radicals in the human body through antioxidant effects and reduce the synthesis of melanin in cells, thereby achieving the effect of whitening.

[0059] Yeast polypeptide is a kind of polypeptide. When added to skin care products, it mainly promotes metabolism, stimulates cell regeneration, and proliferates new collagen. It is mainly a skin conditioner. It is a core ingredient in skin care products that truly plays the role of anti-aging, whitening, wrinkle removal, etc.

[0060] Silk fibroin, also known as fibroin, is a natural high-molecular fibrous protein extracted from silk, accounting for about 70% to 80% of silk. In terms of reducing scar formation after surgery and trauma, silk fibroin helps improve the appearance of scars and skin texture by regulating the activity of fibroblasts and the deposition of collagen. It is also used to fight wrinkles and improve skin sagging, providing a smoother and firmer skin appearance.

[0061] The products prepared in the above Examples 1-3 and Comparative Examples 1-3 were tested, and the results are as follows:

[0062] 60 volunteers with an average age of 20 to 40 years old and problems such as epidermal pigmentation and scars were selected. The volunteers were randomly divided into 6 groups, with 10 people in each group. There was no statistically significant difference in the baseline data of the volunteers in each group (P>0.05); the volunteers in each group applied the dressings prepared by Examples 1 to 3 and Comparative Examples 1 to 3, respectively. Usage: After cleaning the face, the volunteers sprayed the dressing on the face, sprayed twice a day, and used it continuously for 14 days. During the experiment, the subjects could not apply any other cosmetics on the experimental area.

[0063] The volunteers were scored based on the morphology of skin lesions and the degree of scars / pigmentation on a scale of 0 to 10. The skin condition of the volunteers was observed under natural light. The higher the score, the better the improvement effect on scars / pigmentation; the lower the score, the worse the effect of the dressing on improving the volunteers' pigmentation and scars.

[0064] The evaluation results are shown in the following table, where the scores are average scores (1-10 points).

[0065] The test results are shown in the following table:

[0066] Treatment Day 3 Treatment day 7 Treatment day 10 Treatment day 14 Example 1 4.7 6.1 7.3 8.2 Comparative Example 1 3.8 4.0 5.2 7 Example 2 5.1 6.8 7.6 8.9 Comparative Example 2 4.3 5.0 6.5 7.3 Example 3 5.6 6.9 7.9 9.2 Comparative Example 3 5 6.0 7.5 8.4

[0067] From the above results, it can be seen that the whitening polypeptide dressing of the present invention can effectively inhibit the activity of tyrosinase, thereby inhibiting the production of melanin, and promoting the shedding of melanin to reduce pigmentation, thereby playing a role in whitening and lightening the skin. The feedback results after the trial showed that the polypeptide dressing prepared in Example 3 was more popular with volunteers.

[0068] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

[0069] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide dressing with whitening effect, characterized in that: In terms of mass percentage, the whitening components and contents of the polypeptide dressing are as follows: 0.2%-0.6% of Glehnia littoralis extract, 0.1%-1% of glutathione, 0.1%-0.5% of yeast polypeptide, 0.1%-0.5% of oligopeptide-1, 1%-3% of niacinamide, 0.1%-1% of silk fibroin, 0.01%-0.1% of recombinant human collagen, and the balance of water.

2. A polypeptide dressing with whitening effect according to claim 1, characterized in that: The polypeptide dressing also includes a moisturizer, a preservative and a thickener.

3. A polypeptide dressing with whitening effect according to claim 2, characterized in that: The thickener is carbomer homopolymer, and the mass percentage of the thickener is 0.15%-0.2%.

4. The polypeptide dressing with whitening effect according to claim 2, characterized in that: The ingredients and mass percentages of the moisturizing agent are: 0.05%-0.2% sodium hyaluronate, 1%-3% mussel mucin, and 3%-5% glycerol.

5. The polypeptide dressing with whitening effect according to claim 2, characterized in that: The preservative is methylparaben, and the mass percentage of the preservative is 0.15%-0.2%.

6. The method for preparing the polypeptide dressing according to any one of claims 1 to 5, characterized in that: The following steps are involved: 1) Preparation of solution 1: weigh a certain amount of purified water and pour it into a water pot, add the extract of Glehnia littoralis, glutathione, yeast polypeptide, oligopeptide-1, niacinamide, silk fibroin, recombinant human collagen, sodium hyaluronate, mussel mucin and triethanolamine, stir and dissolve for later use; 2) Preparation of solution 2: weigh a certain amount of purified water and pour it into the main pot, add glycerin, methylparaben and carbomer homopolymer, heat at 80°C for 35 minutes and then start stirring at a speed of 1400 rpm for 10 minutes; 3) Homogenization and emulsification: Suck the prepared solution in the water pot into the main pot, set the temperature of the main pot to 80°C, the homogenization speed to 2500rpm, homogenize for 10 minutes, keep warm for 30 minutes, and discharge after cooling.