Anti-scar hydrogel for repairing skin wounds and preparation method of anti-scar hydrogel

Abstract

The invention relates to the field of skin wound repair, and particularly discloses an anti-scar hydrogel for skin wound repair and a preparation method thereof.The preparation method comprises the steps that 1, 10 g of silicon dioxide nanospheres with the average particle size being 50-500 nanometers are selected as a template; step 2, dispersing the template in 1000ml of aqueous solution containing 100-300g of acrylic acid monomer to obtain solution A; 3, 3-9 g of ammonium persulfate is added into the solution A for a polymerization reaction, the reaction temperature ranges from 30 DEG C to 50 DEG C, the pH ranges from 6.5 to 7.5, the reaction time ranges from 2 h to 6 h, and a solution B is obtained after the reaction; active components such as gentamicin, thrombin and epidermal growth factors are uniformly dispersed in the porous hydrogel, the components have a synergistic effect, wound healing is effectively promoted, scar formation is inhibited, gentamicin has an antibacterial effect, and the risk of wound infection can be reduced; thrombin can accelerate blood coagulation and reduce bleeding; and the epidermal growth factor can promote the growth of epithelial cells and accelerate wound healing.

Description

Technical Field

[0001] The invention belongs to the field of skin wound repair, in particular to a scar-proof skin wound repair hydrogel and a preparation method thereof. Background Art

[0002] Skin wound repair is a complex and delicate physiological process involving multiple stages such as inflammation, fibrosis, neovascularization, collagen deposition, epithelialization, and wound contraction. Problems at any stage in the healing process can lead to chronic wound formation, increase the risk of bacterial infection, and may cause scarring. For healthy individuals, the normal healing process occurs at an optimal rate, but in chronic wounds it is often delayed or even completely impaired.

[0003] Traditional skin wound repair technologies often focus on wound healing, while the inhibition of scar formation is relatively weak. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides an anti-scar skin wound repair hydrogel to solve the problem that traditional skin wound repair technology in the prior art often focuses on wound healing while the inhibition of scar formation is relatively weak.

[0005] A method for preparing a hydrogel for scar prevention and skin trauma repair, comprising: Step 1: Select 10 g of silica nanospheres with an average particle size of 50-500 nm as a template; Step 2: dispersing the template in 1000 ml of an aqueous solution containing 100-300 g of an acrylic acid monomer to obtain a solution A; Step 3: Add 3-9 g of ammonium persulfate to the solution A for polymerization reaction at a reaction temperature of 30-50° C., a pH of 6.5-7.5, and a reaction time of 2-6 h to obtain a solution B; Step 4: adding 50-400 ml of hydrofluoric acid solution to the solution B, wherein the mass concentration of the hydrofluoric acid solution should be 5%-20%, to ensure that the silica nanosphere template can be completely removed to obtain a hydrogel with a porous structure; Step 5: washing the hydrogel obtained in step 4 with water to remove unreacted acrylic acid monomer and hydrofluoric acid residue, and then drying naturally at room temperature; Step 6: Mix gentamicin, thrombin and epidermal growth factor according to the following mass ratio: 1 part of gentamicin, 0.5-2 parts of thrombin, and 0.1-1 parts of epidermal growth factor, and then evenly disperse the mixed active ingredients in the hydrogel obtained in step 5 at a ratio of 2% of the total mass of the hydrogel; Step 7: Add silicone to the hydrogel obtained in step 6, the amount of silicone added is 5%-20% of the total mass of the hydrogel, and stir to make it evenly dispersed to enhance the anti-scar effect; Step 8: Post-processing the hydrogel obtained in step 7, including washing again to remove the non-adsorbed active ingredients, and then drying and curing, and then packaging and sterilizing.

[0006] Preferably, in step 1, the average particle size of the silica nanospheres is 150-250 nanometers.

[0007] Preferably, in step 2, the concentration of the aqueous solution containing acrylic acid monomer is 15%-25%.

[0008] Preferably, in step three, the polymerization reaction is carried out for 3-5 hours.

[0009] Preferably, in step 4, the concentration of the hydrofluoric acid solution is 10%-15%.

[0010] A scar-proof skin wound repairing hydrogel prepared by the preparation method.

[0011] Compared with the prior art, the present invention has the following beneficial effects: By evenly dispersing active ingredients such as gentamicin, thrombin and epidermal growth factor in the porous hydrogel, these ingredients can work synergistically to effectively promote wound healing and inhibit scar formation. Gentamicin has antibacterial effects and can reduce the risk of wound infection; thrombin can accelerate blood coagulation and reduce bleeding; epidermal growth factor can promote epithelial cell growth and accelerate wound healing; At the same time, adding silicone ingredients can further enhance the anti-scar effect. Silicone has excellent film-forming and breathable properties, and can form a protective film on the wound surface to reduce water evaporation and external stimulation, providing a moist environment for wound healing, thereby helping to reduce scar formation; The hydrogel material prepared by the invention has good biocompatibility and can directly contact with human tissue without causing adverse reactions. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the preparation process of the present invention. DETAILED DESCRIPTION

[0013] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0014] like Figure 1 As shown: Embodiment 1: The present invention provides a method for preparing a scar-proof skin wound repair hydrogel, comprising: Step 1: Select 10 g of silica nanospheres with an average particle size of 50 nanometers as a template; Step 2: dispersing the template in 1000 ml of an aqueous solution containing 100 g of acrylic acid monomer to obtain a solution A; Step 3: Add 3 g of ammonium persulfate to the solution A for polymerization reaction at a reaction temperature of 30° C., a pH of 6.5, and a reaction time of 2 h to obtain a solution B; Step 4: adding 50 ml of hydrofluoric acid solution to the solution B, wherein the mass concentration of the hydrofluoric acid solution should be 5%, to ensure that the silica nanosphere template can be completely removed to obtain a hydrogel with a porous structure; Step 5: washing the hydrogel obtained in step 4 with water to remove unreacted acrylic acid monomer and hydrofluoric acid residue, and then drying naturally at room temperature; Step 6: Mix gentamicin, thrombin and epidermal growth factor according to the following mass ratio: 1 part of gentamicin, 0.5 part of thrombin, and 0.1 part of epidermal growth factor, and then evenly disperse the mixed active ingredients in the hydrogel obtained in step 5 at a ratio of 2% of the total mass of the hydrogel; Step 7: Add silicone to the hydrogel obtained in step 6, the amount of silicone added is 5% of the total mass of the hydrogel, and stir to make it evenly dispersed to enhance the anti-scar effect; Step 8: Post-processing the hydrogel obtained in step 7, including washing again to remove the non-adsorbed active ingredients, and then drying and curing, and then packaging and sterilizing.

[0015] Embodiment 2: The present invention provides a method for preparing a scar-proof skin wound repair hydrogel, comprising: Step 1: Select 10 g of silica nanospheres with an average particle size of 200 nanometers as a template; Step 2: dispersing the template in 1000 ml of an aqueous solution containing 200 g of acrylic acid monomer (acrylic acid monomer concentration is 20%) to obtain solution A; Step 3: Add 6 g of ammonium persulfate to the solution A for polymerization reaction at a reaction temperature of 40° C., a pH of 7, and a reaction time of 4 hours to obtain a solution B; Step 4: adding 250 ml of hydrofluoric acid solution to the solution B, wherein the concentration of the hydrofluoric acid solution is 12.5%, to ensure that the silica nanosphere template can be completely removed to obtain a hydrogel with a porous structure; Step 5: washing the hydrogel obtained in step 4 with water to remove unreacted acrylic acid monomer and hydrofluoric acid residue, and then drying naturally at room temperature; Step 6: Mix gentamicin, thrombin and epidermal growth factor according to the following mass ratio: 1 part of gentamicin, 0.5 part of thrombin, and 0.1 part of epidermal growth factor, and then evenly disperse the mixed active ingredients in the hydrogel obtained in step 5 at a ratio of 2% of the total mass of the hydrogel; Step 7: Add silicone to the hydrogel obtained in step 6, the amount of silicone added is 5% of the total mass of the hydrogel, and stir to make it evenly dispersed to enhance the anti-scar effect; Step 8: Post-processing the hydrogel obtained in step 7, including washing again to remove the non-adsorbed active ingredients, and then drying and curing, and then packaging and sterilizing.

[0016] Embodiment 3: The present invention provides a method for preparing a scar-proof skin wound repair hydrogel, comprising: A method for preparing a hydrogel for scar prevention and skin wound repair, comprising: Step 1: Select 10 g of silica nanospheres with an average particle size of 500 nanometers as a template; Step 2: dispersing the template in 1000 ml of an aqueous solution containing 300 g of an acrylic acid monomer (acrylic acid monomer concentration is 30%) to obtain a solution A; Step 3: Add 9 g of ammonium persulfate to the solution A for polymerization reaction at a reaction temperature of 50° C., a pH of 7.5, and a reaction time of 6 hours to obtain a solution B; Step 4: adding 400 ml of hydrofluoric acid solution to the solution B, wherein the concentration of the hydrofluoric acid solution is 20%, to ensure that the silica nanosphere template can be completely removed to obtain a hydrogel with a porous structure; Step 5: washing the hydrogel obtained in step 4 with water to remove unreacted acrylic acid monomer and hydrofluoric acid residue, and then drying naturally at room temperature; Step 6: Mix gentamicin, thrombin and epidermal growth factor according to the following mass ratio: 1 part of gentamicin, 0.5 part of thrombin, and 0.1 part of epidermal growth factor, and then evenly disperse the mixed active ingredients in the hydrogel obtained in step 5 at a ratio of 2% of the total mass of the hydrogel; Step 7: Add silicone to the hydrogel obtained in step 6, the amount of silicone added is 5% of the total mass of the hydrogel, and stir to make it evenly dispersed to enhance the anti-scar effect; Step 8: Post-processing the hydrogel obtained in step 7, including washing again to remove the non-adsorbed active ingredients, and then drying and curing, and then packaging and sterilizing.

[0017] Experimental example: Experiment Introduction Animal models: Rats, mice, and pigs were selected as models to simulate human skin wounds and deep wounds, respectively.

[0018] Groups: Divided into a control group and an experimental group. The control group used commercially available skin repair products or standard medical care methods, and the experimental group used the hydrogel prepared by the present invention.

[0019] Observation indicators include wound healing time, scar area, skin elasticity, pigmentation, dermis regeneration and subcutaneous tissue repair.

[0020] Data Collection: Experimental data were collected through regular observation and recording, as well as histological examination.

[0021]

[0022]

[0023]

[0024] Experimental data analysis Healing time: The healing time of animals in the experimental group was significantly shorter than that in the control group, indicating that the hydrogel can effectively promote the healing of skin wounds.

[0025] Scar area: The scar area of ​​the experimental group animals was significantly smaller than that of the control group, indicating that the hydrogel can significantly reduce scar formation.

[0026] Skin elasticity: The skin elasticity of animals in the experimental group was better than that in the control group, indicating that the hydrogel can significantly improve skin elasticity.

[0027] Pigmentation: The pigmentation of animals in the experimental group was significantly lighter than that in the control group, indicating that the hydrogel can significantly reduce pigmentation.

[0028] Dermis regeneration and subcutaneous tissue repair: The dermis regeneration and subcutaneous tissue repair of the experimental group of animals were better than those of the control group, indicating that the hydrogel can significantly improve the regeneration and repair of the dermis and subcutaneous tissue.

[0029] The standard parts used in the present invention can all be purchased from the market, and the special-shaped parts can be customized according to the description and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0030] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0031] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0033] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0034] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0035] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a hydrogel for scar prevention and skin wound repair, characterized in that: include: Step 1: Select 10 g of silica nanospheres with an average particle size of 50-500 nm as a template; Step 2: dispersing the template in 1000 ml of an aqueous solution containing 100-300 g of an acrylic acid monomer to obtain a solution A; Step 3: Add 3-9 g of ammonium persulfate to the solution A for polymerization reaction at a reaction temperature of 30-50° C., a pH of 6.5-7.5, and a reaction time of 2-6 h to obtain a solution B; Step 4: adding 50-400 ml of hydrofluoric acid solution to the solution B, wherein the mass concentration of the hydrofluoric acid solution should be 5%-20%, to ensure that the silica nanosphere template can be completely removed to obtain a hydrogel with a porous structure; Step 5: washing the hydrogel obtained in step 4 with water to remove unreacted acrylic acid monomer and hydrofluoric acid residue, and then drying naturally at room temperature; Step 6: Mix gentamicin, thrombin and epidermal growth factor according to the following mass ratio: 1 part of gentamicin, 0.5-2 parts of thrombin, and 0.1-1 parts of epidermal growth factor, and then evenly disperse the mixed active ingredients in the hydrogel obtained in step 5 at a ratio of 2% of the total mass of the hydrogel; Step 7: Add silicone to the hydrogel obtained in step 6, the amount of silicone added is 5%-20% of the total mass of the hydrogel, and stir to make it evenly dispersed to enhance the anti-scar effect; Step 8: Post-processing the hydrogel obtained in step 7, including washing again to remove the non-adsorbed active ingredients, and then drying and curing, and then packaging and sterilizing.

2. The method for preparing a scar-proof skin wound repair hydrogel according to claim 1, characterized in that: In step 1, the average particle size of the silica nanospheres is 150-250 nanometers.

3. The method for preparing a scar-proof skin wound repairing hydrogel as claimed in claim 1, characterized in that: In step 2, the concentration of the aqueous solution containing acrylic acid monomer is 15%-25%.

4. The method for preparing a scar-proof skin wound repairing hydrogel according to claim 1, characterized in that: In step three, the polymerization reaction is carried out for 3-5 hours.

5. The method for preparing a scar-proof skin wound repairing hydrogel according to claim 1, characterized in that: In step 4, the concentration of the hydrofluoric acid solution is 10%-15%.

6. An anti-scar skin wound repairing hydrogel prepared by the preparation method according to any one of claims 1 to 5.

Images