Bacillus subtilis composition for treating false smut and preparation method thereof

Abstract

The invention relates to the technical field of agricultural biology, and discloses a bacillus subtilis composition for treating false smut, which is prepared from the following raw materials in parts by weight: 30 to 55 parts of bacillus subtilis strain A, 30 to 55 parts of bacillus subtilis strain B, 10 to 15 parts of auxiliaries, 20 to 30 parts of culture medium, 5 to 15 parts of acid-base solution and 60 to 70 parts of distilled water, the auxiliary agent is prepared from 1.5 to 3 parts of dispersing agent, 2 to 3.5 parts of wetting agent, 4 to 5 parts of stabilizing agent and 2.5 to 3.5 parts of carrier. By providing an appropriate growth environment, promoting rapid propagation and metabolism, accurately adjusting the pH value and reasonably controlling the dissolved oxygen level, the bacillus subtilis has stronger adaptability and can be massively propagated, enough metabolites and bioactive substances are generated, the growth and propagation of ustilaginoidea virens are directly inhibited, the morbidity and illness state of the ustilaginoidea virens can be effectively reduced, and the quality of the ustilaginoidea virens is improved. The problem that a traditional prevention and treatment method is poor in effect is solved.

Description

Technical Field

[0001] The invention relates to the field of agricultural biotechnology, in particular to a Bacillus subtilis composition for treating rice false smut and a preparation method thereof. Background Art

[0002] Rice false smut is one of the common and serious diseases in the growth process of rice. It not only reduces the yield of rice, but also affects the quality of rice. In order to effectively prevent and control rice false smut, researchers are constantly exploring new methods. Among them, the use of Bacillus subtilis compositions for treatment has gradually attracted attention. Bacillus subtilis has good biocontrol effects, can inhibit the growth of pathogens, regulate plant physiological metabolism, and enhance plant immunity.

[0003] However, the existing Bacillus subtilis compositions for treating rice false smut have insufficient strain activity and stability in some products, resulting in poor control effects. In addition, previous methods for controlling rice false smut are costly and complex to operate, and traditional control methods bring about environmental pollution, pesticide residues in agricultural products, destruction of ecological balance, and food safety risks. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a Bacillus subtilis composition for treating rice false smut and a preparation method thereof, which solves the problems of low activity of the Bacillus subtilis composition, high production cost and pesticide residues.

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: A Bacillus subtilis composition for treating rice false smut, comprising the following raw materials in parts by weight: 30 to 55 parts of Bacillus subtilis strain A, 30 to 55 parts of Bacillus subtilis strain B, 10 to 15 parts of an adjuvant, 20 to 30 parts of a culture medium, 5 to 15 parts of an acid-base solution, and 60 to 70 parts of distilled water;

[0006] The auxiliary agent includes 1.5 to 3 parts of a dispersant, 2 to 3.5 parts of a wetting agent, 4 to 5 parts of a stabilizer and 2.5 to 3.5 parts of a carrier;

[0007] The culture medium comprises 5 to 7 parts of carbon source, 10 to 15 parts of nitrogen source and 5 to 8 parts of inorganic salt.

[0008] Preferably, the dispersant is sodium lignin sulfonate, the wetting agent is tea saponin, the stabilizer is xanthan gum, and the carrier is diatomaceous earth;

[0009] The carbon source is glucose, the nitrogen source is peptone, and the inorganic salt is magnesium salt.

[0010] A method for preparing a Bacillus subtilis composition for treating rice false smut, the method comprising the following steps:

[0011] Step 1, adding distilled water to the culture medium, stirring evenly, adjusting the pH value, sterilizing for standby use, and then inoculating Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and cultivation, the culture conditions are temperature 30-35° C., rotation speed 180-200 rpm, and culture time 24-48 hours;

[0012] Step 2, the cultured strain A and strain B are transferred to separate fermentation tanks for fermentation, the temperature during the fermentation process is 30-35° C., the pH value is 6.8-7.2, and the dissolved oxygen is 28%-32%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.0-7.2 to obtain a seed solution;

[0013] Step 3, inoculating the seed solution in step 3 into the fermentation medium at an inoculum amount of 5% to 15%, and culturing to the logarithmic growth phase under the conditions of a temperature of 35 to 37° C., a rotation speed of 180 to 200 rpm, and a ventilation volume of 1.0 to 1.5 vvm;

[0014] Step 4. After the fermentation is completed, the bacteria are collected by centrifugation at a speed of 8000-10000 rpm for 15-20 minutes. The collected bacteria are then fully mixed with the auxiliary agent at a mass ratio of 1: (10-15) at a stirring speed of 500-800 rpm for 30-60 minutes, and finally sterilized.

[0015] Preferably, the culture medium and distilled water are sterilized before use.

[0016] Preferably, the culture medium sterilization comprises the following steps:

[0017] Place the prepared culture medium into a high temperature and high pressure sterilization pot, close the lid of the sterilization container, open the exhaust valve, and start heating the sterilization container;

[0018] When the exhaust valve discharges a large amount of steam and the cold air in the sterilization container is completely discharged, close the exhaust valve and continue heating to raise the pressure in the sterilization container to 0.10-0.11MPa and the temperature to 121-126℃, and maintain for 15-30 minutes;

[0019] After the sterilization time is reached, stop heating, wait for the pressure in the sterilization container to naturally drop to zero, open the lid of the sterilization container, take out the sterilized culture medium, and place it in a sterile environment to cool for later use;

[0020] The distilled water sterilization comprises the following steps:

[0021] Put the distilled water into a container that can withstand high temperature and high pressure, and then put the container containing the distilled water into the sterilizer;

[0022] According to the above-mentioned medium sterilization operation steps, the process of exhausting, increasing pressure, maintaining pressure and reducing pressure is carried out, and the sterilization pressure, temperature and time conditions are consistent with the medium sterilization;

[0023] After sterilization, take out the sterilized distilled water under sterile conditions for use.

[0024] Preferably, the acid-base solution has a concentration of 0.1 mol / L to 0.5 mol / L.

[0025] Preferably, in step 2, distilled water is added to the culture medium, stirred evenly, the pH value is adjusted to 7.0-7.5, and the ventilation volume during inoculation culture is 0.5-1.0 vvm.

[0026] Preferably, in the step 5, after the stirring is completed, the obtained mixed solution is cooled to room temperature in a sterile environment, thereby obtaining a Bacillus subtilis composition for treating rice false smut.

[0027] The present invention provides a Bacillus subtilis composition for treating rice false smut, which has the following beneficial effects:

[0028] 1. The present invention combines Bacillus subtilis strain A and strain B to provide a suitable growth environment for the culture and fermentation of the strains, promote their rapid reproduction and metabolism, and accurately adjust the pH value to create an optimal acid-base environment for the strains, reasonably control the dissolved oxygen level, promote growth and metabolism, and make Bacillus subtilis more adaptable and able to reproduce in large quantities, produce sufficient metabolites and bioactive substances, directly inhibit the growth and reproduction of rice false smut fungi, effectively reduce the incidence and condition of rice false smut, and solve the problem of poor effect of traditional prevention and control methods.

[0029] 2. The present invention has strict and detailed sterilization requirements in the culture medium, distilled water and strain culture and fermentation links. By accurately controlling the temperature, pH value, dissolved oxygen and ventilation volume parameters, the stability and reliability of the production process quality of the composition are guaranteed, thereby improving production efficiency and helping to reduce production costs, making this method for preventing and controlling rice false smut more cost-effective, thereby solving the problems of high cost and complex operation of previous prevention and control methods.

[0030] 3. The present invention adopts biological Bacillus subtilis as the main ingredient. Bacillus subtilis is a biological preparation, which reduces the use of chemical pesticides and has the advantages of reducing environmental pollution and pesticide residues in agricultural products. It effectively protects the ecological balance and food safety, provides people with greener and healthier agricultural products, and promotes the sustainable development of agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The present invention is a flow chart of a method for preparing a Bacillus subtilis composition for treating rice false smut; DETAILED DESCRIPTION

[0032] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Example:

[0034] Please see attached Figure 1 The embodiment of the present invention provides a Bacillus subtilis composition for treating rice false smut, comprising the following raw materials in parts by weight: 30 to 55 parts of Bacillus subtilis strain A, 30 to 55 parts of Bacillus subtilis strain B, 10 to 15 parts of an adjuvant, 20 to 30 parts of a culture medium, 5 to 15 parts of an acid-base solution, and 60 to 70 parts of distilled water;

[0035] The auxiliary agent includes 1.5 to 3 parts of dispersant, 2 to 3.5 parts of wetting agent, 4 to 5 parts of stabilizer and 2.5 to 3.5 parts of carrier;

[0036] The culture medium comprises 5 to 7 parts of a carbon source, 10 to 15 parts of a nitrogen source and 5 to 8 parts of an inorganic salt.

[0037] The dispersant is sodium lignin sulfonate, the wetting agent is tea saponin, the stabilizer is xanthan gum, and the carrier is diatomaceous earth;

[0038] The carbon source is glucose, the nitrogen source is peptone, and the inorganic salt is magnesium salt.

[0039] A method for preparing a Bacillus subtilis composition for treating rice false smut, the method comprising the following steps:

[0040] Step 1, adding distilled water to the culture medium, stirring evenly, adjusting the pH value, sterilizing for standby use, and then inoculating Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and cultivation, the culture conditions are temperature 30-35° C., rotation speed 180-200 rpm, and culture time 24-48 hours;

[0041] Step 2, the cultured strain A and strain B are transferred to separate fermentation tanks for fermentation, the temperature during the fermentation process is 30-35° C., the pH value is 6.8-7.2, and the dissolved oxygen is 28%-32%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.0-7.2 to obtain a seed solution;

[0042] Step 3, inoculating the seed solution in step 3 into the fermentation medium at an inoculum amount of 5% to 15%, and culturing to the logarithmic growth phase under the conditions of a temperature of 35 to 37° C., a rotation speed of 180 to 200 rpm, and a ventilation volume of 1.0 to 1.5 vvm;

[0043] Step 4. After the fermentation is completed, the bacteria are collected by centrifugation at a speed of 8000-10000 rpm for 15-20 minutes. The collected bacteria are then fully mixed with the auxiliary agent at a mass ratio of 1: (10-15) at a stirring speed of 500-800 rpm for 30-60 minutes, and finally sterilized.

[0044] The culture medium and distilled water must be sterilized before use.

[0045] Sterilization of culture medium includes the following steps:

[0046] Place the prepared culture medium into a high temperature and high pressure sterilization pot, close the lid of the sterilization container, open the exhaust valve, and start heating the sterilization container;

[0047] When the exhaust valve discharges a large amount of steam and the cold air in the sterilization container is completely discharged, close the exhaust valve and continue heating to raise the pressure in the sterilization container to 0.10-0.11MPa and the temperature to 121-126℃, and maintain for 15-30 minutes;

[0048] After the sterilization time is reached, stop heating, wait for the pressure in the sterilization container to naturally drop to zero, open the lid of the sterilization container, take out the sterilized culture medium, and place it in a sterile environment to cool for later use;

[0049] Sterilization of distilled water includes the following steps:

[0050] Put the distilled water into a container that can withstand high temperature and high pressure, and then put the container containing the distilled water into the sterilizer;

[0051] According to the above-mentioned medium sterilization operation steps, the process of exhausting, increasing pressure, maintaining pressure and reducing pressure is carried out, and the sterilization pressure, temperature and time conditions are consistent with the medium sterilization;

[0052] After sterilization, take out the sterilized distilled water under sterile conditions for use.

[0053] The concentration of the acid and alkali solution is 0.1 mol / L to 0.5 mol / L.

[0054] In step 2, distilled water is added to the culture medium, stirred evenly, the pH value is adjusted to 7.0-7.5, and the ventilation volume during inoculation culture is 0.5-1.0 vvm.

[0055] In step 5, after the stirring is completed, the obtained mixed solution is cooled to room temperature in a sterile environment to obtain a Bacillus subtilis composition for treating rice false smut.

[0056] Sodium lignin sulfonate can evenly disperse Bacillus subtilis in the preparation, ensuring that the active ingredients are evenly distributed when applied to the field, thus improving the consistency of the control effect;

[0057] Tea saponin, a wetting agent, helps to increase the wetting and adhesion ability of the preparation on the surface of plant leaves, making it easier for Bacillus subtilis to colonize and take effect, thus enhancing the control effect;

[0058] The stabilizer xanthan gum can maintain the physical stability of the preparation, prevent the active ingredients from settling or deteriorating, and extend the shelf life and use effect of the product;

[0059] The carrier diatomaceous earth has good adsorption properties and can carry Bacillus subtilis and its metabolites. After being applied to the field, it provides a certain living environment and nutritional support for the strain, enhancing its activity and durability.

[0060] The carbon source glucose in the culture medium provides an energy source for the growth and metabolism of Bacillus subtilis, promoting the rapid reproduction of the strain;

[0061] Nitrogen source peptone, which provides nitrogen required for the synthesis of cell substances and supports cell growth and division;

[0062] Inorganic magnesium salts participate in the activation and regulation of multiple enzymes, maintain the normal physiological functions of cells, and ensure the normal growth and metabolic activities of Bacillus subtilis.

[0063] The following is an introduction in conjunction with specific embodiments:

[0064] Embodiment 1:

[0065] Prepare the following raw materials in parts by weight: 30 parts of Bacillus subtilis strain A, 30 parts of Bacillus subtilis strain B, 10 parts of auxiliary agent, 20 parts of culture medium, 5 parts of acid-base solution, and 60 parts of distilled water;

[0066] Step 1: Add distilled water to the culture medium, stir evenly, adjust the pH value to 7.0, sterilize and set aside, then inoculate Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and culture, the culture conditions are temperature 30°C, rotation speed 180rpm, culture time 24 hours, ventilation volume 0.5vvm;

[0067] Step 2: The cultured strain A and strain B are transferred to separate fermentation tanks for fermentation. The temperature during the fermentation process is 30° C., the pH value is 6.8, and the dissolved oxygen is 28%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.0 to obtain a seed solution;

[0068] Step 3: inoculate the seed solution in step 3 into the fermentation medium at an inoculum rate of 5%, and culture to the logarithmic growth phase under the conditions of a temperature of 35° C., a rotation speed of 180 rpm, and a ventilation rate of 1.0 vvm;

[0069] Step 4: After the fermentation is completed, the cells are collected by centrifugation, the centrifugal speed is 8000rpm, the centrifugal time is 15 minutes, and then the collected cells are fully mixed with the auxiliary agent according to the mass ratio of 1:10, the stirring speed is 500rpm, the stirring time is 30 minutes, and finally sterilized. The auxiliary agent includes 1.5 parts of dispersant, 2 parts of wetting agent, 4 parts of stabilizer, and 2.5 parts of carrier, the culture medium includes 5 parts of carbon source, 10 parts of nitrogen source, and 5 parts of inorganic salt, and the concentration of acid-base solution is 0.1mol / L. After stirring, the obtained mixed solution is cooled to room temperature in a sterile environment, that is, the Bacillus subtilis composition for treating rice false smut is obtained.

[0070] Embodiment 2:

[0071] Prepare the following raw materials in parts by weight: 42 parts of Bacillus subtilis strain A, 42 parts of Bacillus subtilis strain B, 12 parts of auxiliary agent, 25 parts of culture medium, 10 parts of acid-base solution, and 65 parts of distilled water;

[0072] Step 1: Add distilled water to the culture medium, stir evenly, adjust the pH value to 7.2, sterilize and set aside, then inoculate Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and culture, the culture conditions are temperature 32°C, rotation speed 190rpm, culture time 36 hours, ventilation volume 0.75vvm;

[0073] Step 2: The cultured strain A and strain B are transferred to separate fermentation tanks for fermentation. The temperature during the fermentation process is 32° C., the pH value is 7.0, and the dissolved oxygen is 30%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.1 to obtain a seed solution;

[0074] Step 3: inoculate the seed solution in step 3 into the fermentation medium at an inoculum rate of 10%, and culture to the logarithmic growth phase under the conditions of a temperature of 36° C., a rotation speed of 190 rpm, and a ventilation volume of 1.25 vvm;

[0075] Step 4: After the fermentation is completed, the bacteria are collected by centrifugation, the centrifugal speed is 9000rpm, the centrifugal time is 18 minutes, and then the collected bacteria are fully mixed with the auxiliary agent at a mass ratio of 1:12, the stirring speed is 650rpm, the stirring time is 45 minutes, and finally sterilized. The auxiliary agent includes 2.2 parts of dispersant, 2.7 parts of wetting agent, 4.5 parts of stabilizer, and 3 parts of carrier. The culture medium includes 6 parts of carbon source, 12 parts of nitrogen source, and 6 parts of inorganic salt. The concentration of acid and alkali solution is 0.3 mol / L. After stirring, the obtained mixed solution is cooled to room temperature in a sterile environment to obtain a Bacillus subtilis composition for treating rice false smut.

[0076] Embodiment three:

[0077] Prepare the following raw materials in parts by weight: 55 parts of Bacillus subtilis strain A, 55 parts of Bacillus subtilis strain B, 15 parts of auxiliary agent, 30 parts of culture medium, 15 parts of acid-base solution, and 70 parts of distilled water;

[0078] Step 1: Add distilled water to the culture medium, stir evenly, adjust the pH value to 7.5, sterilize and set aside, then inoculate Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and culture, the culture conditions are temperature 35°C, rotation speed 200rpm, culture time 48 hours, ventilation volume 1.0vvm;

[0079] Step 2: The cultured strain A and strain B are transferred to separate fermentation tanks for fermentation. The temperature during the fermentation process is 35° C., the pH value is 7.2, and the dissolved oxygen is 32%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.2 to obtain a seed solution;

[0080] Step 3: inoculate the seed solution in step 3 into the fermentation medium at an inoculum rate of 15%, and culture to the logarithmic growth phase under the conditions of a temperature of 37° C., a rotation speed of 200 rpm, and a ventilation volume of 1.5 vvm;

[0081] Step 4: After the fermentation is completed, the bacteria are collected by centrifugation, the centrifugal speed is 10000rpm, the centrifugal time is 20 minutes, and then the collected bacteria are fully mixed with the auxiliary agent at a mass ratio of 1:15, the stirring speed is 800rpm, the stirring time is 60 minutes, and finally sterilized. The auxiliary agent includes 3 parts of dispersant, 3.5 parts of wetting agent, 5 parts of stabilizer, and 3.5 parts of carrier. The culture medium includes 7 parts of carbon source, 15 parts of nitrogen source, and 8 parts of inorganic salt. The concentration of acid and alkali solution is 0.5 mol / L. After stirring, the obtained mixed solution is cooled to room temperature in a sterile environment to obtain a Bacillus subtilis composition for treating rice false smut.

[0082] Table 1 shows that by using raw materials of different weight proportions and different step data during processing, the corresponding proportion can be selected according to different disease degrees to ensure that no waste is caused and the growth and reproduction of rice smut fungus is inhibited.

[0083] Comparison of inhibition and treatment rates in Examples 1 to 3 Table 1:

[0084]

[0085]

[0086] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Bacillus subtilis composition for treating rice false smut, characterized in that: The invention comprises the following raw materials in parts by weight: 30 to 55 parts of Bacillus subtilis strain A, 30 to 55 parts of Bacillus subtilis strain B, 10 to 15 parts of auxiliary agent, 20 to 30 parts of culture medium, 5 to 15 parts of acid-base solution and 60 to 70 parts of distilled water; The auxiliary agent includes 1.5 to 3 parts of a dispersant, 2 to 3.5 parts of a wetting agent, 4 to 5 parts of a stabilizer and 2.5 to 3.5 parts of a carrier; The culture medium comprises 5 to 7 parts of carbon source, 10 to 15 parts of nitrogen source and 5 to 8 parts of inorganic salt.

2. The Bacillus subtilis composition for treating rice false smut according to claim 1, characterized in that: The dispersant is sodium lignin sulfonate, the wetting agent is tea saponin, the stabilizer is xanthan gum, and the carrier is diatomaceous earth; The carbon source is glucose, the nitrogen source is peptone, and the inorganic salt is magnesium salt.

3. A method for preparing a Bacillus subtilis composition for treating rice false smut, characterized in that: A Bacillus subtilis composition for treating rice false smut as claimed in any one of claims 1 to 2, the method comprising the following steps: Step 1, adding distilled water to the culture medium, stirring evenly, adjusting the pH value, sterilizing for standby use, and then inoculating Bacillus subtilis strain A and Bacillus subtilis strain B into the sterilized culture medium for inoculation and cultivation, the culture conditions are temperature 30-35° C., rotation speed 180-200 rpm, and culture time 24-48 hours; Step 2, the cultured strain A and strain B are transferred to separate fermentation tanks for fermentation, the temperature during the fermentation process is 30-35° C., the pH value is 6.8-7.2, and the dissolved oxygen is 28%-32%. During the fermentation, an acid-base solution is used to adjust the pH value of the overall environment of the Bacillus subtilis strain A and strain B in the fermentation tank to 7.0-7.2 to obtain a seed solution; Step 3, inoculating the seed solution in step 3 into the fermentation medium at an inoculum amount of 5% to 15%, and culturing to the logarithmic growth phase under the conditions of a temperature of 35 to 37° C., a rotation speed of 180 to 200 rpm, and a ventilation volume of 1.0 to 1.5 vvm; Step 4. After the fermentation is completed, the bacteria are collected by centrifugation at a speed of 8000-10000 rpm for 15-20 minutes. The collected bacteria are then fully mixed with the auxiliary agent at a mass ratio of 1: (10-15) at a stirring speed of 500-800 rpm for 30-60 minutes, and finally sterilized.

4. The Bacillus subtilis composition for treating rice false smut according to claim 1, characterized in that: The culture medium and distilled water must be sterilized before use.

5. The Bacillus subtilis composition for treating rice false smut according to claim 4, characterized in that: The culture medium sterilization comprises the following steps: Place the prepared culture medium into a high temperature and high pressure sterilization pot, close the lid of the sterilization container, open the exhaust valve, and start heating the sterilization container; When the exhaust valve discharges a large amount of steam and the cold air in the sterilization container is completely discharged, close the exhaust valve and continue heating to raise the pressure in the sterilization container to 0.10-0.11MPa and the temperature to 121-126℃, and maintain for 15-30 minutes; After the sterilization time is reached, stop heating, wait for the pressure in the sterilization container to naturally drop to zero, open the lid of the sterilization container, take out the sterilized culture medium, and place it in a sterile environment to cool for later use; The distilled water sterilization comprises the following steps: Put the distilled water into a container that can withstand high temperature and high pressure, and then put the container containing the distilled water into the sterilizer; According to the above-mentioned medium sterilization operation steps, the process of exhausting, increasing pressure, maintaining pressure and reducing pressure is carried out, and the sterilization pressure, temperature and time conditions are consistent with the medium sterilization; After sterilization, take out the sterilized distilled water under sterile conditions for use.

6. The Bacillus subtilis composition for treating rice false smut according to claim 1, characterized in that: The acid-base solution has a concentration of 0.1 mol / L to 0.5 mol / L.

7. The Bacillus subtilis composition for treating rice false smut according to claim 1, characterized in that: In the step 2, distilled water is added to the culture medium, stirred evenly, the pH value is adjusted to 7.0-7.5, and the ventilation volume during inoculation culture is 0.5-1.0 vvm.

8. The Bacillus subtilis composition for treating rice false smut according to claim 1, characterized in that: In the step 5, after the stirring is completed, the obtained mixed solution is cooled to room temperature in a sterile environment, and the Bacillus subtilis composition for treating rice false smut is obtained.

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