Microsphere type antibacterial gel as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of external medicine compositions, and particularly relates to microsphere type antibacterial gel as well as a preparation method and application thereof. The preparation comprises three parts, namely dextran microspheres wrapped by polyethylene glycol, a mixed antibacterial component and a carbomer mixed solution. The mixed antibacterial component is prepared from thymol, chlorhexidine acetate, cinnamyl aldehyde, citral, gallic acid and nano silver. Glucan is prepared into microspheres by utilizing a micro-fluidic chip technology, antibacterial components are wrapped in the microspheres, then the microspheres are compounded into the microsphere type antibacterial gel, and the gel not only shows excellent antibacterial performance and strong adhesive capacity, but also has a remarkable wound repair promoting effect; the microsphere structure can wrap and mix the antibacterial components and play a role in controlled release of the antibacterial components, so that a novel solution is provided for the medical field of antibacterial gel.

Description

Technical Field

[0001] The invention belongs to the technical field of external medicine compositions, and specifically relates to a microsphere antibacterial gel and a preparation method and application thereof. Background Art

[0002] Antibacterial gel is a topical gel preparation with antibacterial effect, mainly used to prevent and treat bacterial infections on the skin surface.

[0003] Traditional wound repair methods often rely on gauze and bandages. However, these materials lack the ability to actively promote repair, so their contribution to wound healing is relatively limited. During the replacement process, they may inadvertently cause secondary trauma to the wound. Their main function is to simply seal the wound, but they are not very effective in maintaining a suitable moist environment for the wound and effectively inhibiting bacteria.

[0004] Therefore, the development of new wound dressings has become a top priority, which not only carries important medical value, but also contains broad market potential. As a water-rich material, hydrogel has properties that are highly similar to human tissues, and some types show excellent biocompatibility and certain antibacterial ability, providing a more ideal environment for wound repair and effectively avoiding the limitations of traditional repair materials. However, unoptimized hydrogels are still insufficient in antibacterial efficacy and adhesion stability, and it is difficult to meet the stringent requirements of actual clinical applications. Summary of the invention

[0005] In order to address the deficiencies of the prior art, the present invention provides a microsphere antibacterial gel and a preparation method thereof, which not only exhibits excellent antibacterial performance and strong adhesion ability, but also has a significant wound repair promoting effect; the microsphere structure can encapsulate the mixed antibacterial components and play a role in the sustained and controlled release of the antibacterial components, thereby bringing a new solution to the medical field of antibacterial gel.

[0006] The technical solution of the present invention is as follows:

[0007] The first aspect of the present invention provides a microsphere antibacterial gel, wherein the microsphere gel comprises polyethylene glycol-coated dextran microspheres, a mixed antibacterial component and a carbomer mixed solution, specifically:

[0008] Phase A: Dextran

[0009] Phase B: Polyethylene glycol

[0010] Phase C: mixed antibacterial ingredients

[0011] Phase D: Carbomer mixture

[0012] The C phase is wrapped by the A phase. Through the microfluidic chip technology, the A phase (wrapping the C phase) and the B phase fluid are injected into the microfluidic channel. The A phase (wrapping the C phase) fluid is sheared by the B phase fluid and forms monodisperse microspheres with a diameter in the micron level. From a macroscopic perspective, the A phase (wrapping the C phase) microspheres float in the B phase to form a microsphere suspension; the microsphere suspension is wrapped by the D phase.

[0013] The mixed antibacterial ingredients include: thymol, chlorhexidine acetate, cinnamaldehyde, citral, gallic acid and nano silver.

[0014] Furthermore, the mass ratio of the mixed antibacterial components is thymol: chlorhexidine acetate: cinnamaldehyde: citral: gallic acid: nanosilver = (0.2-0.3): (0.1-0.2): (1): (0.2-0.3): (0.15-0.25): (0.01-0.05).

[0015] Furthermore, the mass ratio of the components in the carbomer mixture is disodium EDTA:chitosan:carbomer:propylene glycol:glycerol=(0.02-0.06):(1-3):(0.4-1):(3):(1-3).

[0016] The second aspect of the present invention provides a method for preparing the microsphere antibacterial gel according to the first aspect, comprising the following steps:

[0017] a) Preparation of antibacterial solution

[0018] dissolving the mixed antibacterial components in water to prepare an antibacterial solution;

[0019] b) Preparation of microspheres

[0020] Dextran powder (10 wt%) and polyethylene glycol powder (10 wt%) were dissolved in water to prepare a dextran-polyethylene glycol mixed solution.

[0021] After the above powders were dissolved, the solution was centrifuged (1000 rpm, 2 h) and then allowed to stand for 24 hours. At this time, the dextran solution and the polyethylene glycol solution were phase separated with a clear fluid interface between the two. A pipette was used to collect the dextran solution and the polyethylene glycol solution, respectively.

[0022] Add phase C to the dextran solution and stir thoroughly to obtain a uniformly mixed phase A.

[0023] Phase A and phase B are introduced into the microfluidic chip. Under the microfluidic hydrodynamic mode, phase B shears phase A into microspheres to obtain a microsphere suspension.

[0024] c) Carbomer mixture coating

[0025] Disodium EDTA, chitosan, carbomer, propylene glycol and glycerol are dissolved in water to prepare a carbomer mixture, and then the microsphere suspension is added to the carbomer mixture to obtain a microsphere antibacterial gel.

[0026] Furthermore, in the preparation of the antibacterial solution a), the total concentration of the mixed antibacterial components is 2-8 wt %.

[0027] Furthermore, in the preparation of the microspheres b), the mass ratio of dextran powder to polyethylene glycol powder is 1:1, the concentration of dextran is 10 wt %, and the concentration of polyethylene glycol is 10 wt %.

[0028] Furthermore, in the preparation of b) microspheres, the diameter of the microspheres is 5 μm-800 μm.

[0029] Furthermore, in the coating of the carbomer mixture c), the volume ratio of the microsphere suspension to the carbomer mixture is (1-3): (7-9).

[0030] Furthermore, in the coating of the carbomer mixture c), the volume ratio of the microsphere suspension to the carbomer mixture is 1:4.

[0031] The second aspect of the present invention provides the use of the microsphere antibacterial gel described in the first aspect in the preparation of wound repair materials and adhesive materials.

[0032] Furthermore, the wound repair material and adhesive material are used to prevent and treat bacterial infections on the skin surface.

[0033] The present invention generates microspheres by microfluidic technology, encapsulates mixed antibacterial agents in the microspheres, and finally prepares microsphere carbomer gel. Compared with simple carbomer gel, the present invention can retain more antibacterial components, increase the concentration, and achieve sustained and controlled release of antibacterial components by means of the microsphere structure.

[0034] Antibacterial ingredients in the present invention:

[0035] Thymol: Thymol is an organic compound with significant antibacterial effects. It can effectively inhibit the growth of a variety of bacteria and fungi, causing bacterial death by destroying the permeability of cell membranes. It is widely used in food preservation, medical bactericides and other fields. In addition, thymol also has the characteristics of low toxicity, low residue and easy degradation in the environment.

[0036] Chlorhexidine acetate: Chlorhexidine acetate is a broad-spectrum antibacterial agent with powerful bactericidal, disinfectant and antiseptic effects. It can destroy the cell membrane structure of bacteria and effectively kill a variety of Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa. It is often used for disinfection of skin, wounds and instruments before surgery, as well as antiseptic treatment of medical devices and drugs.

[0037] Cinnamaldehyde: Cinnamaldehyde is an aromatic aldehyde extracted from cinnamon bark with excellent antibacterial properties. It can effectively inhibit the growth of a variety of bacteria and fungi, including common pathogens such as Escherichia coli and Staphylococcus aureus. The antibacterial mechanism of cinnamaldehyde is that the aldehyde group in its molecule can combine with the hydrophilic group on the surface of bacteria, penetrate the cell wall, and destroy the cell structure, thereby achieving an antibacterial or bactericidal effect. In addition, cinnamaldehyde is non-toxic or low-toxic to the human body, so it is widely used in food mildew inhibitors, medicine and other fields. It is a safe and effective antibacterial agent.

[0038] Citral: Citral is a natural organic compound with significant antibacterial properties. It can destroy bacterial cell membranes, inhibit their growth and reproduction, and effectively fight against a variety of pathogenic microorganisms, including bacteria, fungi and viruses. It is an ideal antibacterial agent for medical equipment disinfection and various fields.

[0039] Gallic acid: Gallic acid is an organic acid with broad-spectrum antibacterial properties, which can effectively inhibit the growth of various bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, etc. Its antibacterial mechanism includes destroying bacterial cell membranes, interfering with bacterial metabolism and anti-oxidation, thus exerting a significant bactericidal effect.

[0040] Nanosilver: Nanosilver is a new type of antibacterial material obtained by nano-sizing silver using cutting-edge nanotechnology. Its particle size is mostly around 25 nanometers, and it has strong antibacterial and bactericidal properties. The antibacterial principle of nanosilver is that it can penetrate the cell membrane of microorganisms, bind to the enzymes in the cells, inactivate the enzymes, and thus block the respiratory metabolic system of the microorganisms, leading to their death. In addition, nanosilver also has a broad-spectrum antibacterial property, which can effectively fight against a variety of bacteria, fungi, viruses and other microorganisms, and will not produce drug resistance.

[0041] Given that each antibacterial ingredient has different focal points, simply increasing the concentration of a single ingredient to enhance the antibacterial effect is often ineffective and may aggravate the irritation problem. Therefore, the present invention cleverly selects multiple antibacterial ingredients with synergistic effects, and through their mutual cooperation, a more excellent antibacterial effect is achieved while maintaining or reducing the overall usage.

[0042] One or more technical solutions of the present invention described above achieve the following beneficial effects:

[0043] 1. The present invention uses microspheres prepared by microfluidic technology as a wrapping scaffold for the antibacterial components, and suspends the microspheres in a carbomer gel solution, thereby further enhancing the antibacterial effect of the gel, which has a wide range of practical application value.

[0044] 2. In the present invention, thymol, chlorhexidine acetate, cinnamaldehyde, citral, gallic acid, and nano-silver are compounded into gel as antibacterial ingredients, which significantly alleviates the skin irritation that may be caused by high concentrations of a single ingredient, and greatly prolongs the antibacterial time of the product. The prepared microsphere gel not only exhibits excellent antibacterial performance, but also has the mild characteristics of low irritation to wounds, providing a safer and more efficient antibacterial solution for antibacterial gel products. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0046] Figure 1 Diagram of the device for generating microfluidic microspheres;

[0047] Figure 2 for the microsphere generation process;

[0048] Figure 3 This is a scanning electron microscope image of the microspheres. DETAILED DESCRIPTION

[0049] In order to better understand the present invention, the present invention is further described below in conjunction with specific serial numbers, wherein the terms used in the serial numbers are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0050] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0051] The present invention is further described in conjunction with specific examples. The following examples are only for explaining the present invention and are not intended to limit the content thereof. If the specific experimental conditions are not specified in the examples, they are usually carried out according to conventional conditions or the conditions recommended by the reagent company; the reagents and consumables used in the following examples, unless otherwise specified, can all be obtained from commercial sources.

[0052] The sources of some raw materials used in the present invention are as follows: Table 1:

[0053] name Product Name / Model / Other Name Source (Manufacturer) Dextran Dextran (DEX) Aladdin Reagents (Shanghai) Co., Ltd. Polyethylene glycol PEG Dow Chemical (China) Investment Co., Ltd. Thymol 5-Isopropyl-2-methylphenol Hubei Yuancheng Saichuang Technology Co., Ltd. Chlorhexidine acetate Bis(chlorobenzyl)biguanide Xi'an Libang Pharmaceutical Co., Ltd. Cinnamaldehyde β-phenylacrolein Hubei Jusheng Technology Co., Ltd. Citral Geranial Firmenich Fragrances (China) Co., Ltd. gallic acid 3,4,5-Trihydroxybenzoic acid Tianjin Jindong Tianzheng Fine Chemical Reagent Factory Nano Silver AgNPs (Silver Nanoparticles) Suzhou Nanotech Co., Ltd. Disodium EDTA Disodium EDTA Sigma-Aldrich (Shanghai) Trading Co., Ltd. Chitosan Chitosan Zhejiang Golden Shell Biochemical Co., Ltd. Carbomer Carbomer U20 Nouyo Chemical (Shanghai) Co., Ltd. Propylene glycol Methyl glycol Dow Chemical (China) Investment Co., Ltd. glycerin Glycerol Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd.

[0054] Example 1: Preparation of microsphere antibacterial gel

[0055] a) Preparation of antibacterial solution

[0056] dissolving the mixed antibacterial components in water to prepare an antibacterial solution;

[0057] b) Preparation of microspheres (see Figure 1 , Figure 2 , Figure 3 )

[0058] Dextran powder (10 wt%) and polyethylene glycol powder (10 wt%) were dissolved in water to prepare a dextran-polyethylene glycol mixed solution.

[0059] After the above powders were dissolved, the solution was centrifuged (1000 rpm, 2 h) and then allowed to stand for 24 hours. At this time, the dextran solution and the polyethylene glycol solution were phase separated, with a clear fluid interface between the two.

[0060] Using a pipette, collect the dextran solution and polyethylene glycol solution separately.

[0061] Add phase C to the dextran solution and stir thoroughly to obtain a uniformly mixed phase A.

[0062] The microsphere preparation process is achieved through a microfluidic system. The microfluidic microsphere generation device is as follows Figure 1 As shown, it includes a pressure pump 1 and a pressure controller 2 connected by a gas pipeline 3, an A-phase liquid storage bottle 4, an A-phase fluid 6, a B-phase liquid storage bottle 5, a B-phase fluid 7, and a microfluidic chip 9 connected by a fluid pipeline 8. In the microfluidic chip 9, 10 is the generated microspheres. The A-phase and B-phase fluids finally flow into a collection bottle 11, 10 is the collected microspheres, and 12 is the B-phase solution.

[0063] Phase A and phase B are added into the above-mentioned microfluidic system. Under the microfluidic hydrodynamic mode, phase B shears phase A into microspheres to obtain a microsphere suspension.

[0064] The microsphere generation process is as follows Figure 2 As shown, microfluidic chips of different sizes can generate microspheres of different sizes, and the microspheres contain mixed antibacterial components.

[0065] Scanning electron microscope images of microspheres Figure 3 As shown, the microspheres have good stability and have nanosilver particles on the surface of the microspheres.

[0066] c) Carbomer mixture coating

[0067] Disodium EDTA, chitosan, carbomer, propylene glycol, and glycerol are dissolved in water to prepare a carbomer mixture. Then, the microsphere suspension is added to the carbomer mixture to obtain a microsphere antibacterial gel. The mass ratio of the total weight of the microsphere suspension to the carbomer solution is 1:4.

[0068] Example 2-7: Preparation of microsphere antibacterial gels with different ratios

[0069] Preparation method: Same as Example 1.

[0070] The specific ratio is shown in Table 2.

[0071] Table 2 Preparation ratio of microsphere antibacterial gel

[0072]

[0073]

[0074] Note: The units in the table are parts by mass, and the concentration of antibacterial solution is 1%.

[0075] Example 7-11: Preparation of microsphere antibacterial gel with different antibacterial concentrations

[0076] Preparation method: Same as Example 1.

[0077] The antibacterial gel ratio is the same as that of Example 6, except that the concentrations of the antibacterial solution are set to 2%, 3%, 5%, 8%, and 10%, respectively, as shown in Table 3 below.

[0078] Table 3 Concentrations of different antibacterial solutions

[0079]

[0080] Comparative Examples 1-5: Preparation of antibacterial gels with different ratios

[0081] Experimental method: Preparation of antibacterial gel

[0082] a) Preparation of antibacterial solution

[0083] The mixed antibacterial components are dissolved in water to prepare an antibacterial solution, the formulation of which is shown in Table 3 below;

[0084] b) Preparation of gel skeleton

[0085] Take dextran and place it in water for later use;

[0086] Dissolve sodium alginate in water containing dextran solution, adjust the pH value to 6-9, and form a sodium alginate-dextran complex for later use. The mass ratio of sodium alginate to dextran is 1:1;

[0087] c) Gel coating

[0088] The sodium alginate-dextran complex is placed in an antibacterial solution, disodium EDTA, chitosan, carbomer, propylene glycol, and glycerol are dissolved in water to prepare a carbomer mixed solution, the antibacterial solution is added to the carbomer mixed solution, and stirred to obtain an antibacterial gel. The total weight of the carbomer solution is 4 times the total weight of the sodium alginate-dextran antibacterial solution; the specific ratio is shown in Table 4.

[0089] Table 4 Preparation ratio of microsphere antibacterial gel that was not generated

[0090]

[0091]

[0092] Note: The units in the table are parts by mass, and the concentration of antibacterial solution is 1%.

[0093] Comparative Examples 6-10: Preparation of microsphere antibacterial gels with different ratios

[0094] Preparation method: Same as Example 1.

[0095] The specific ratio is shown in Table 5.

[0096] Table 5 Preparation ratio of microsphere antibacterial gel

[0097]

[0098] Note: / in the table indicates no addition, all units are parts by mass, and the concentration of antibacterial solution in the table is 1%.

[0099] Sample stability test:

[0100] 1. Heat resistance test: Set the constant temperature incubator to 40°C. Then, for the 10 samples prepared previously, take out three of each sample and put them into transparent glass bottles. The sample volume of each bottle is ensured to be 20 ml. After the sample is loaded and sealed, place the glass bottles in the constant temperature incubator. After three months of continuous testing, take out the samples and wait for them to return to room temperature. Then carefully observe and record the changes in the appearance of each sample.

[0101] 2. Cold resistance test: Adjust the constant temperature incubator to -10℃. For each of the 10 samples prepared previously, take out three samples and put them into transparent glass bottles, ensuring that the sample volume of each bottle is 20 ml. After sealing, place the glass bottle in the adjusted constant temperature incubator. After three months of low temperature testing, take out the samples and restore them to room temperature, then carefully observe and record the changes in the appearance of each sample.

[0102] 3. Normal temperature test: For the 10 samples prepared previously, take out three samples of each sample and put them into transparent glass bottles, and keep the sample volume of each bottle at 20 ml. After the samples are filled and sealed, place the samples at normal room temperature. After one month of storage, take out the samples and directly observe whether there are any changes in their appearance.

[0103] No precipitation, precipitate, cracking, water loss, or damage was observed in the heat resistance test, cold resistance test, and room temperature test, and the original appearance was maintained. At the same time, the internal microsphere rupture rate was less than 5%, and the antibacterial ingredients were well wrapped and protected.

[0104] Human skin patch test

[0105] The five groups of samples numbered 1 to 5 were tested according to the human skin patch test method specified in the "2022 Cosmetic Safety Technical Specifications". During the test, the skin reactions of the subjects must be carefully observed according to standard procedures at three specific time points - 30 minutes (after the indentation completely subsides), 24 hours and 48 hours, and the results of each observation must be recorded in detail. After rigorous observation and recording, it was confirmed that at the three evaluation nodes of 30 minutes, 24 hours and 48 hours, the skin reaction level of all subjects was level 0, indicating that the samples did not cause any adverse reactions in the test.

[0106] Antibacterial activity evaluation

[0107] The antibacterial activity of the 10 groups of samples obtained was determined by the improved filter paper method (similar to the traditional method), and the diameter of the inhibition zone was used as the main evaluation index to quantify the antibacterial effect.

[0108] The specific steps are:

[0109] Preparation of antibacterial tablets: accurately cut the antibacterial gel products corresponding to each serial number into circular thin slices with a diameter of 6 mm, and use them as antibacterial tablets for later use, ensuring that each tablet is of the same size.

[0110] Preparation of bacterial plates: First, select the strain to be tested and culture it overnight in a nutrient-rich medium to promote its growth. Second, dilute the cultured strain with phosphate buffered saline (PBS) to an appropriate concentration (10 6 cfu / mL) to ensure uniform distribution of the bacterial solution. Use the plate coating method to evenly coat the diluted bacterial solution on the surface of the nutrient agar plate to ensure uniform coverage without omissions. Finally, place the coated plate at room temperature to dry naturally for about 5 minutes to allow the bacterial solution to fully adsorb on the agar surface.

[0111] Antibacterial effect test: First, gently place the prepared antibacterial sheet on the stained agar plate to ensure that the antibacterial sheet fits tightly against the bacterial layer to avoid bubbles. Then, place the fitted plate in a constant temperature incubator and set a suitable temperature for incubation, usually for 18 hours, to observe the antibacterial effect. Finally, after the incubation, use a vernier caliper to accurately measure the outer diameter of the antibacterial circle (i.e., the antibacterial diameter, in millimeters) and record the data. To ensure the accuracy of the experimental results, each test group must be measured three times, and the average value must be calculated as the basis for evaluating the final antibacterial diameter.

[0112] The results are shown in Table 6.

[0113] Table 6 Antibacterial results

[0114]

[0115] It can be seen from Table 6 that the 10% antibacterial solution concentration of Example 11 has the best antibacterial effect, but considering that too high a concentration may cause irritation, and the 10% antibacterial concentration of Example 11 is not significantly different from the 8% antibacterial solution concentration of Example 10; and the 1% concentration of Example 6 is compared with the 2% concentration of Example 7, the effect is quite different, so the concentration of the antibacterial solution of the present invention is limited to 2-8%.

[0116] From the comparison of the above results, it can be seen that when the concentration of the antibacterial solution is within 2-8%, better antibacterial effect can be achieved when the antibacterial components are encapsulated by microspheres.

[0117] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microsphere antibacterial gel, characterized in that: The microsphere gel comprises polyethylene glycol-coated dextran microspheres, mixed antibacterial components and a carbomer mixed solution; The mixed antibacterial ingredients include: thymol, chlorhexidine acetate, cinnamaldehyde, citral, gallic acid and nano silver.

2. The microsphere antibacterial gel according to claim 1, characterized in that: The mass ratio of the mixed antibacterial components is thymol: chlorhexidine acetate: cinnamaldehyde: citral: gallic acid: nanosilver = (0.2-0.3): (0.1-0.2): (1): (0.2-0.3): (0.15-0.25): (0.01-0.05).

3. The microsphere antibacterial gel according to claim 1, characterized in that: The mass ratio of the components in the carbomer mixture is disodium EDTA:chitosan:carbomer:propylene glycol:glycerol=(0.02-0.06):(1-3):(0.4-1):(3):(1-3).

4. The method for preparing the microsphere antibacterial gel according to any one of claims 1 to 3, characterized in that: The following steps are involved: a) Preparation of antibacterial solution dissolving the mixed antibacterial components in water to prepare an antibacterial solution; b) Preparation of microspheres Take dextran powder and polyethylene glycol powder, dissolve them in water to prepare a dextran-polyethylene glycol mixed solution; The dextran-polyethylene glycol mixed solution is centrifuged and allowed to stand, and the dextran solution and the polyethylene glycol solution are collected respectively; Add phase C to the dextran solution and stir to obtain a uniformly mixed phase A; Passing the uniformly mixed phase A and phase B into a microfluidic chip to obtain a microsphere suspension; c) Carbomer mixture coating Disodium EDTA, chitosan, carbomer, propylene glycol and glycerin are dissolved in water to prepare a carbomer mixture; The microsphere suspension is added into the carbomer mixed solution to obtain a microsphere antibacterial gel.

5. The method for preparing the microsphere antibacterial gel according to claim 4, characterized in that: In the preparation of the antibacterial solution a), the total concentration of the mixed antibacterial components is 2-8 wt %.

6. The method for preparing the microsphere antibacterial gel according to claim 4, characterized in that: In the preparation of b) microspheres, the mass ratio of dextran powder to polyethylene glycol powder is 1:

1.

7. The method for preparing the microsphere antibacterial gel according to claim 4, characterized in that: In the preparation of b) microspheres, the diameter of the microspheres is 5 μm-800 μm.

8. The method for preparing the microsphere antibacterial gel according to claim 4, characterized in that: In the coating of the carbomer mixture c), the volume ratio of the microsphere suspension to the carbomer mixture is (1-3): (7-9).

9. Use of the microsphere antibacterial gel according to any one of claims 1 to 3 in the preparation of wound repair materials and adhesive materials.

10. The use according to claim 9, characterized in that: The wound repair material and the adhesive material are used for preventing and treating bacterial infection on the skin surface.

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