Simple field fungal quantification pinning
By using a dual distance control and protection system consisting of a cannula and a limiting rod, the problems of inconsistent inoculation needle depth and contamination by miscellaneous bacteria in the field environment are solved, achieving simplicity and accuracy in field fungal inoculation.
Patent Information
- Application Number
- CN202411550201.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-01
- Publication Date
- 2025-11-21
- Estimated Expiration
- 2044-11-01
AI Technical Summary
Existing inoculation needles cannot be used in the field, and it is difficult for multiple inoculation needles to maintain a consistent depth when piercing the fruit peel, which can easily lead to contamination by other microorganisms.
A simple field fungal quantitative inoculation needle was designed, which uses dual distance control of the cannula and the limiting rod, combined with a liquid quantitative control flow meter, to ensure that multiple puncture bodies maintain a consistent puncture depth on the fruit, and prevents contamination by other fungi through protective components.
It achieves consistent inoculation depth and resistance to contamination by multiple inoculation needles in field operations, and is easy to operate and highly adaptable.
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Figure CN119391521B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of fungal inoculation tools, and more particularly to a simple field fungal quantitative inoculation needle. Background Technology
[0002] Currently, existing inoculation needles are only suitable for laboratory use and not for inoculation operations in the field. Furthermore, when multiple inoculation needles pierce the fruit peel for inoculation, they cannot adapt to the curvature of the fruit surface, which can easily lead to inconsistent penetration depths when multiple inoculation needles are used.
[0003] Developing a simple field fungal quantitative inoculation needle that can prevent contamination by other fungi during field inoculation operations and ensure consistent penetration depth into the fruit peel when multiple needles are used simultaneously has become a technical challenge that urgently needs to be solved by those skilled in the art. Summary of the Invention
[0004] The purpose of this invention is to provide a simple quantitative inoculation needle for field fungi, thereby solving the problems listed in the background art.
[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:
[0006] The present invention provides a simple field fungal quantitative inoculation needle, comprising a connector, the upper end of which is connected to the working end of a liquid quantitative control flow meter, the lower end of which is connected to the upper end of a connecting tube, the outer circumferential surface of the connecting tube being slidably connected to a sleeve, the lower end of the sleeve being connected to a puncture body, and a fastening nut being movably installed at the upper end of the sleeve.
[0007] Preferably, the upper end of the sleeve is conical, and an external thread is formed on the outer circumferential surface of the upper end of the sleeve.
[0008] Preferably, a connecting plate is installed on the outer circumferential surface of the lower end of the sleeve, and the connecting plate is threadedly connected to the limiting rod.
[0009] Preferably, it also includes a protective component, which is fitted onto the outer peripheral surface of the puncture body.
[0010] Preferably, the protective component includes an elastic cavity that abuts against the upper end of the telescopic tube, and a rubber head is installed at the lower end of the telescopic tube;
[0011] Furthermore, the lower surface of the rubber head is flush with the needle tip of the puncture body, and the inner ring wall of the rubber head abuts against the outer wall of the puncture body.
[0012] Compared with the prior art, the beneficial technical effects of the present invention are as follows:
[0013] The present invention provides a simple field fungal quantitative inoculation needle. Through dual distance control of the cannula and the limiting rod, it is possible to ensure that the piercing depth of multiple puncture bodies remains consistent when multiple puncture bodies are used to inoculate fruits with fungi, and the operation is convenient.
[0014] Meanwhile, the retractable protective components can meet the inoculation environment of the field, effectively blocking external bacteria and demonstrating strong environmental adaptability. Attached Figure Description
[0015] The present invention will be further described below with reference to the accompanying drawings.
[0016] Figure 1 This is a schematic diagram of the main view of the simple field fungal quantitative inoculation needle of the present invention;
[0017] Figure 2 This is a schematic diagram illustrating the working state of the simple field fungal quantitative inoculation needle of the present invention. Figure 1 ;
[0018] Figure 3 This is a schematic diagram illustrating the working state of the simple field fungal quantitative inoculation needle of the present invention. Figure 2 ;
[0019] Figure 4 This is a cross-sectional schematic diagram of the simple field fungal quantitative inoculation needle of the present invention;
[0020] Figure 5 This is a schematic diagram illustrating the working state of the simple field fungal quantitative inoculation needle of the present invention. Figure 3 .
[0021] Explanation of reference numerals in the attached drawings: 1. Connector; 2. Connecting tube; 3. Fastening nut; 4. Sleeve; 5. Puncture body; 6. Elastic cavity; 7. Telescopic tube; 8. Rubber head; 9. Connecting plate; 10. Limiting rod. Detailed Implementation
[0022] To make the technical problems to be solved, the technical solutions, and the beneficial effects of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0023] like Figure 1-5 As shown, a simple field fungal quantitative inoculation needle includes a connector 1. The upper end of the connector 1 is connected to the working end of a liquid quantitative control flow meter. The lower end opening of the connector 1 is connected to the upper end of a connecting tube 2. The outer circumferential surface of the connecting tube 2 is slidably connected to a sleeve 4. The lower end of the sleeve 4 is connected to a puncture body 5, and a fastening nut 3 is movably installed on the upper end of the sleeve 4.
[0024] By connecting to a liquid quantitative control flow meter, quantitative control of fungal culture medium can be achieved, so that the flow rate of the inoculation needle is 1-10 microliters.
[0025] The cannula can move the puncture body up and down along the outer circumference of the connecting tube, so that when multiple inoculation needles inoculate the fruit at the same time, the depth to which each puncture body penetrates the fruit peel is consistent.
[0026] Specifically, the upper end of the sleeve 4 is conical, and an external thread is provided on the outer circumference of the upper end of the sleeve 4. After the sleeve moves a certain distance along the connecting pipe, it is fixedly connected to the upper end of the sleeve by tightening the nut. Furthermore, a sealing sleeve is provided at the joint between the upper end of the sleeve and the connecting pipe to ensure the sealing performance between the sleeve and the connecting pipe.
[0027] Specifically, a connecting plate 9 is installed on the outer circumferential surface of the lower end of the sleeve 4. The connecting plate 9 is threadedly connected to the limiting rod 10, keeping the lower end of the limiting rod and the needle tip of the piercing body on the same horizontal plane. When it is necessary to adjust the depth of piercing into the fruit peel, the limiting rod is adjusted upward. At this time, the height difference between the lower end of the limiting rod and the needle tip of the piercing body is the depth of piercing into the fruit.
[0028] Furthermore, scale lines are engraved on the outer circumference of the limit rod, with the zero scale line of the limit rod located at the lower end of the limit rod.
[0029] Specifically, it also includes a protective component, which is fitted onto the outer peripheral surface of the puncture body 5. The protective component can effectively block the needle of the puncture body and prevent the puncture site of the puncture body from being exposed to the outside air for a long time.
[0030] Specifically, the protective component includes an elastic cavity 6, which abuts against the upper end of a telescopic tube 7. A rubber head 8 is installed at the lower end of the telescopic tube 7. The elastic cavity is fixedly connected to the outer peripheral surface of the puncture body. The telescopic tube can extend and retract relative to the elastic cavity. The opening diameter of the rubber head is slightly smaller than the outer diameter of the puncture body. When the rubber head is located at the needle position of the puncture body, it can achieve the sealing operation and prevent the air containing bacteria from the outside from contacting the inside of the puncture body.
[0031] Furthermore, a sterilization device is placed inside the telescopic tube to disinfect bacteria on the outer surface of the puncture body.
[0032] Furthermore, the lower surface of the rubber head 8 is flush with the needle tip of the puncture body 5, and the inner ring wall of the rubber head 8 abuts against the outer wall of the puncture body 5, which can also block the juice and debris after the puncture body is punctured and placed on the lower surface of the rubber head.
[0033] During the puncture operation, the lower surface of the rubber head contacts the fruit peel. When the inoculation needle is pressed down, the puncture body is pushed out from the lower surface of the rubber head and pierces into the fruit peel to complete the puncture and inoculation operation.
[0034] The method of using the present invention, such as Figure 2-3 As shown:
[0035] When an inoculation needle is installed at the lower end of the liquid metering control flow meter, the depth of penetration into the fruit peel can be controlled by adjusting the height difference between the lower end of the limiting rod and the needle of the puncture body. After adjusting the height, the preset working parameters of the liquid metering control flow meter can be installed to penetrate into the fruit peel.
[0036] When multiple inoculation needles are installed at the lower end of the liquid metering flow meter:
[0037] First, loosen the fastening nut so that the sleeve hangs down naturally and the lower end of the limiting rod abuts against the fruit peel. At this time, the rubber heads of multiple inoculation needles will also abut against the fruit peel.
[0038] Then, tighten the fastening nuts to secure the cannulas and connecting tubes of the multiple inoculation needles.
[0039] Secondly, adjust the limiting rod upwards so that the lower end of the limiting rod is separated from the fruit peel. The difference in distance between the lower end of the limiting rod and the fruit peel is the depth to which the piercing body penetrates the fruit peel.
[0040] Finally, press down on the puncture body, which extends from the rubber head and pierces into the fruit, completing the inoculation operation according to the preset parameters of the liquid metering flow meter.
[0041] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or seal that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or seal.
[0042] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A simple field fungus quantification inoculation needle comprising a connector (1), characterized in that: The upper end of the connector (1) is connected with the working end of the liquid ration control flow meter, the lower end opening of the connector (1) is communicated with the upper end of the connecting pipe (2), the outer peripheral surface of the connecting pipe (2) is slidably connected with the sleeve (4), the lower end of the sleeve (4) is communicated with the puncture body (5), and the upper end of the sleeve (4) movably installs the fastening nut (3); Further comprising a protection assembly sleeved on the outer peripheral surface of the puncture body (5); The protection assembly comprises an elastic cavity (6), the upper end of an elastic tube (7) is abutted with the elastic cavity (6), and the lower end of the elastic tube (7) is installed with a rubber head (8); The lower surface of the rubber head (8) is flush with the needle tip of the puncture body (5), and the inner annular wall of the rubber head (8) is abutted with the outer lateral wall of the puncture body (5); The upper end of the sleeve (4) is conically arranged, and the outer peripheral surface of the upper end of the sleeve (4) is provided with external threads; The outer peripheral surface of the lower end of the sleeve (4) is installed with a connecting plate (9), and the connecting plate (9) is threadedly connected with a limiting rod (10).
Citation Information
Patent Citations
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