Trifluorobenzene pyrimidine-containing pesticide composition for preventing and treating sogatella furcifera, pesticide preparation as well as preparation method and application thereof

Abstract

The invention provides a triflumezopyrim-containing pesticide composition for preventing and treating sogatella furcifera, a pesticide preparation as well as a preparation method and application thereof, and belongs to the technical field of pest prevention and treatment. The nitenpyram and the pymetrozine are compounded with the trifluoro-benzene pyrimidine respectively, a synergistic effect is shown in a certain mass ratio range, the prevention and treatment effect on the sogatella furcifera can be improved (fast-acting property and persistent effect are achieved), the application dosage of chemical agents is reduced on the basis, and the prevention and treatment cost is reduced. The problems of pesticide residues, environmental pollution and the like caused by large-dose application are avoided to a certain extent.

Description

Technical Field

[0001] The invention belongs to the technical field of pest control, and in particular relates to a pesticide composition containing triflubenzuron for controlling white-backed planthoppers, a pesticide preparation, a preparation method and applications thereof. Background Art

[0002] White-backed planthopper (Sogatellafurcifera (Horváth)) is an important pest in rice production in my country. It has the characteristics of small size, strong reproductive capacity, good migration and high outbreak frequency, and can complete its life cycle on 18 crops and barnyard grass. White-backed planthopper is widely distributed in various regions of Asia. The area of ​​occurrence in China is large, which seriously threatens my country's food security and causes huge losses to economic development. In 2020, my country's Ministry of Agriculture and Rural Affairs included white-backed planthopper in the "List of Class I Crop Pests and Diseases" (Announcement No. 333 of the Ministry of Agriculture and Rural Affairs of the People's Republic of China, 2020).

[0003] Triflumezopyrim, molecular formula C 20 H 13 F3N4O2, with a molecular weight of 398.338 and a chemical name of 2,4-dioxy-1-(5-pyrimidinylmethyl)-3-[3-(trifluoromethyl)phenyl]-3,4-dihydro-2H-pyrido[1,2-a]pyrimidine-1-cation-3-carbon anion, is a new type of mesoionic insecticide developed by DuPont in the United States and is an acetylcholine receptor inhibitor or antagonist. Triflubenzuron has strong systemicity and certain contact and stomach poisoning effects, and has a good insecticidal effect in the field. However, the market price is high, and in actual use, a single agent is prone to cause pests to develop resistance, resulting in reduced prevention effectiveness, and increasing the dosage is prone to cause a series of problems such as pesticide residues and environmental pollution.

[0004] In the process of agricultural pest control, the combination of pesticides is one of the most common strategies in current chemical pest control. However, when pesticides with different ingredients are combined, the combined effects are often not all additive, and some are even antagonistic. There are few combinations that have a truly synergistic effect. At present, there are no reports on the combination of triflubenzuron with other pesticides to achieve targeted improvement in the control of white-backed planthoppers. Summary of the invention

[0005] The object of the present invention is to provide a pesticide composition, a pesticide preparation, a preparation method and an application for controlling white-backed planthoppers. The pesticide composition of the present invention can specifically improve the control effect on white-backed planthoppers, reduce the amount of pesticide application, improve crop safety, delay pest resistance and other problems.

[0006] The invention provides a trifluanid-containing pesticide composition for controlling white-backed planthoppers, comprising an effective active ingredient A and an active ingredient B; wherein the active ingredient A is trifluanid, and the active ingredient B is pymetrozine or nitenpyram.

[0007] Preferably, when the active ingredient B is nitenpyram, the mass ratio of trifluanid to nitenpyram is 1:(4-8).

[0008] Preferably, when the active ingredient B is pymetrozine, the mass ratio of trifluanid to pymetrozine is 1:(30-100).

[0009] The present invention also provides a pesticide preparation, which comprises the pesticide composition described in the above technical solution and pharmaceutically acceptable excipients.

[0010] Preferably, the pesticide formulation comprises a suspension concentrate, a dispersible oil suspension concentrate, a suspension seed coating concentrate, a wettable powder or a water dispersible granule.

[0011] The present invention also provides water-dispersible granules containing trifluanid for controlling white-backed planthoppers. The raw materials for preparing the water-dispersible granules include the pesticide composition and auxiliary materials described in the above technical scheme; the auxiliary materials include one or more of alkylnaphthalene sulfonate sodium formaldehyde condensate dispersant, polycarboxylate dispersant, sodium dodecyl sulfate wetting agent, sodium lignin sulfonate dispersant, alkylnaphthalene sulfonate sodium wetting agent, ammonium sulfate disintegrant, white carbon black filler and kaolin filler.

[0012] The present invention also provides a method for preparing the water dispersible granules described in the above technical solution, comprising the following steps:

[0013] The pesticide composition for controlling white-backed planthoppers containing triflubenzuron is mixed with auxiliary materials to obtain a mixed raw material; the obtained mixed raw material is crushed to obtain a mother powder; the mother powder is mixed with water, granulated, dried and sieved to obtain a water-dispersible granule.

[0014] Preferably, the pulverizing operation includes pulverizing using a jet mill.

[0015] Preferably, the particle size of the master powder is 5 to 10 μm.

[0016] The present invention also provides the use of the triflubenzuron-containing pesticide composition for controlling white-backed planthoppers described in the above technical solution, the pesticide preparation described in the above technical solution, the water-dispersible granules described in the above technical solution, or the water-dispersible granules obtained by the preparation method of the water-dispersible granules described in the above technical solution in controlling white-backed planthoppers.

[0017] The present invention provides a pesticide composition for controlling white-backed planthoppers containing trifluanid, a pesticide preparation, a preparation method and an application thereof. The pesticide composition of the present invention compounds nitenpyram or pymetrozine with trifluanid, exhibits a synergistic effect within a certain mass ratio range, can improve the control effect on white-backed planthoppers (with both rapid effect and long-lasting effect), and based on this, reduces the application dosage of chemical agents, thereby avoiding the problems of pesticide residues and environmental pollution caused by large-dose application to a certain extent. DETAILED DESCRIPTION

[0018] The present invention provides a pesticide composition for controlling white-backed planthoppers containing trifluanid, comprising an effective active ingredient A and an active ingredient B; wherein the active ingredient A is trifluanid, and the active ingredient B is pymetrozine or nitenpyram. In a specific embodiment, the white-backed planthoppers may include nymphs and / or adults. In a specific embodiment, the pesticide composition exhibits a synergistic effect compared to a single component.

[0019] In a specific embodiment, when the active ingredient B is nitenpyram, the mass ratio of trifluanid to nitenpyram can be 1:(4-8). In a specific embodiment, the mass ratio of trifluanid to nitenpyram can also be 1:7. In a specific embodiment, when the mass ratio of trifluanid to nitenpyram is 1:7, the pesticide composition exhibits good rapid effect and long-lasting effect.

[0020] In a specific embodiment, when the active ingredient B is pymetrozine, the mass ratio of triflubenzuron to pymetrozine can be 1:(30-100). In a specific embodiment, the mass ratio of triflubenzuron to pymetrozine can also be 1:60. In a specific embodiment, when the mass ratio of triflubenzuron to pymetrozine is 1:60, the pesticide composition exhibits good rapid effect and long-lasting effect.

[0021] The present invention also provides a pesticide preparation, which comprises the pesticide composition described in the above technical solution and pharmaceutically acceptable excipients.

[0022] In a specific embodiment, the dosage form of the pesticide preparation may include a suspension concentrate, a dispersible oil suspension concentrate, a suspension seed coating concentrate, a wettable powder or a water dispersible granule.

[0023] The present invention also provides water-dispersible granules containing trifluanid for controlling white-backed planthoppers. In a specific embodiment, the raw materials for preparing the water-dispersible granules include the pesticide composition and auxiliary materials described in the above technical scheme; in a specific embodiment, the auxiliary materials include one or more of alkylnaphthalene sulfonate sodium formaldehyde condensate dispersant, polycarboxylate dispersant, sodium dodecyl sulfate wetting agent, sodium lignin sulfonate dispersant, alkylnaphthalene sulfonate sodium wetting agent, ammonium sulfate disintegrant, white carbon black filler and kaolin filler.

[0024] In a specific embodiment, when the water dispersible granules are trifluanid / nitenpyram water dispersible granules, the percentage of each component can also be: trifluanid technical (100% off) 4%, nitenpyram technical (100% off) 28%, sodium alkylnaphthalenesulfonate formaldehyde condensate dispersant 3%, polycarboxylate dispersant 3%, sodium lauryl sulfate wetting agent 3%, ammonium sulfate disintegrant 3%, white carbon black filler 20%, and finally kaolin filler is used to make up to 100%.

[0025] In a specific embodiment, when the water dispersible granules are trifluanid / pymetrozine water dispersible granules, the mass ratio of each component can be: trifluanid technical (100% off) 1%, pymetrozine technical (100% off) 60%, sodium alkylnaphthalene sulfonate formaldehyde condensate dispersant 5%, sodium lignin sulfonate dispersant 5%, sodium alkylnaphthalene sulfonate wetting agent 3%, ammonium sulfate disintegrant 5%, and finally white carbon black filler is used to make up to 100%.

[0026] The present invention also provides a method for preparing the water dispersible granules described in the above technical solution, comprising the following steps:

[0027] The pesticide composition for controlling white-backed planthoppers containing triflubenzuron is mixed with auxiliary materials to obtain a mixed raw material; the obtained mixed raw material is crushed to obtain a mother powder; the mother powder is mixed with water, granulated, dried and sieved to obtain a water-dispersible granule.

[0028] In a specific embodiment, the pulverizing operation includes pulverizing using a jet mill.

[0029] In a specific embodiment, the particle size of the master powder may be 5-10 μm, or may be 5 μm.

[0030] The present invention also provides the use of the triflubenzuron-containing pesticide composition for controlling white-backed planthoppers described in the above technical solution, the pesticide preparation described in the above technical solution, the water-dispersible granules described in the above technical solution, or the water-dispersible granules obtained by the preparation method of the water-dispersible granules described in the above technical solution in controlling white-backed planthoppers.

[0031] In order to further illustrate the present invention, the pesticide composition containing trifluanid for controlling white-backed planthopper and its application provided by the present invention are described in detail below in conjunction with the examples, but they should not be construed as limiting the scope of protection of the present invention.

[0032] Example 1

[0033] Indoor biological activity determination of triflupromide mixtures.

[0034] Test drug

[0035] 99% triflumuron technical, Shanghai Titan Technology Co., Ltd.; 97% nitenpyram technical, Jiangsu Changlong Agrochemical Co., Ltd.; 97% pymetrozine technical, Jiangsu Kesheng Group Co., Ltd.

[0036] Test insect source

[0037] The white-backed planthoppers were collected from Baoying Lake Farm in Huai'an City, Jiangsu Province in 2023, and the indoor toxicity test was carried out when they were reared with tillering rice seedlings to the third-instar nymphs of the next generation. Insect rearing conditions: temperature 25±1℃, humidity 70%-80%, photoperiod 16h:8h (L:D).

[0038] 1. Single-dose biological activity assay

[0039] The rice seedlings grown in the greenhouse were pulled out with their roots, cleaned and cut into 10 cm long rooted rice stems, and placed on filter paper in a cool place to dry until there was no visible water on the surface. The test drugs were dissolved in acetone to prepare 4000 mg / L stock solution (pymetrozine was dissolved in dimethyl sulfoxide), and then diluted with 0.1% TritonX-100 aqueous solution to prepare different series of mass concentration solutions according to the preliminary experimental results (the concentration of triflumuron was 1.6 mg / L, 0.8 mg / L, 0.4 mg / L, 0.2 mg / L, 0.1 mg / L, 0.05 mg / L, the concentration of nitenpyram was 6.4 mg / L, 3.2 mg / L, 1.6 mg / L, 0.8 mg / L, 0.4 mg / L, 0.2 mg / L, 0.1 mg / L, and the concentration of pymetrozine was 243 mg / L, 81 mg / L, 27 mg / L, 9 mg / L, 3 mg / L, 1 mg / L). The dried rice stems were evenly divided into groups and immersed in different concentrations of liquid medicine for 30 seconds. They were taken out to dry and the roots were wrapped with wet absorbent cotton to keep them moist. They were placed in plastic cups, with 3 plants per cup. The above operation was repeated in the order of the experimental design dose from low to high. Each concentration was treated 3 times, and a 0.1% TritonX-100 aqueous solution was used as a blank control. The 3rd instar nymphs were moved into plastic cups with a sucker, 15 per cup, 3 replicates, and a total of 45. The cups were plugged with sponges and transferred to a temperature of 25±1℃, a humidity of 70% to 80%, and a photoperiod of 16h:8h (L:D) for observation and feeding. The number of dead insects was checked and recorded at 96h (check results 168h after pymetrozine), and the control mortality rate was less than 10% for an effective test.

[0040] 2. Determination of co-toxicity factors

[0041] Based on the single-dose toxicity test, the active ingredients A (trifluanid) and B (nitenpyram and pymetrozine) for compounding were diluted to LC 50 value dose, and the corresponding LC 50The values ​​are a and b, respectively. Five ratios are set at six equal points, namely a / 5b, a / 2b, a / b, 2a / b, and 5a / b. Mix the dosage solution of a with the dosage solution of b at a volume ratio of 1:5, 1:2, 1:1, 2:1, and 5:1 to obtain mixed solutions of different ratios, with concentrations of (a+5b) / 6, (a+2b) / 3, (a+b) / 2, (2a+b) / 3, and (5a+b) / 6, respectively. The measured mortality rate is the mortality rate obtained in the actual test, and the theoretical mortality rate is the mortality rate calculated by the linear equation of a single dose. Calculate the mortality rate and co-toxicity factor of white-backed planthoppers after 96 hours. A co-toxicity factor greater than 20 indicates a synergistic effect, between -20 and 20 is an additive effect, and a co-toxicity factor less than -20 is an antagonistic effect.

[0042] The calculation formula of the co-toxicity factor is shown in Formula I:

[0043]

[0044] 3. Determination of co-toxicity coefficient

[0045] Referring to the method of Sun et al., the co-toxicity factor method was used to screen out the ratio with a co-toxicity factor greater than 20 as a reference, and the ratio was further refined within this ratio range to determine its toxicity to the third-instar nymphs of white-backed planthoppers. The results of the inspection after 96 hours were used to calculate the toxicity regression equation, slope and LC 50 , screen the combination with a co-toxicity coefficient greater than 120. The relative toxicity index and the mixed toxicity index calculation formula are shown in Formula II and Formula III:

[0046]

[0047] The calculation formula of the co-toxicity coefficient is shown in Formula IV:

[0048]

[0049] Among them, the theoretical toxicity index (TTI) of the mixture = TI A Percentage of agent A in the mixture + TI B The percentage of drug B in the mixture. A co-toxicity coefficient greater than 120 indicates a synergistic effect; between 80 and 120 indicates an additive effect; and less than 80 indicates an antagonistic effect.

[0050] 4. Statistical analysis

[0051] Microsoft Excel 2010 and SPSS19.0 data processing software were used for data processing and analysis, and the toxicity regression equation, lethal concentration LC50, 95% confidence interval, etc. were obtained; the insect population reduction rate and control effect of the field efficacy test were calculated, and the insect population reduction rate and control effect data were arcsine square root transformed, and Dumcan's new multiple range method was used to analyze the significant differences in the control effects of each treatment (p=0.05).

[0052] Table 1 Indoor toxicity of each single agent to the third instar nymphs of white-backed planthopper

[0053]

[0054] As shown in Table 1, the lethal concentrations of trifluanid, nitenpyram and pymetrozine to white-backed planthoppers are 50 They are 0.187mg / L, 0.949mg / L and 13.037mg / L respectively.

[0055] Table 2 Determination of co-toxic factors of various combinations against the 3rd instar nymphs of white-backed planthopper

[0056]

[0057] It can be seen from Table 2 that when trifluanid and nitenpyram are mixed in a ratio of 1:5, the co-toxicity factor is greater than 20, showing a synergistic effect. When trifluanid and pymetrozine are mixed in a ratio of 1:70 and 1:35, the co-toxicity factor is greater than 20, showing a synergistic effect.

[0058] Table 3 Determination of the co-toxicity coefficient of each compound combination against the 3rd instar nymph of white-backed planthopper

[0059]

[0060]

[0061] As can be seen from Table 3, the co-toxicity coefficient of trifluanid and nitenpyram in the ratio of 1:5, 1:6 and 1:7 to white-backed planthopper was greater than 120, showing a synergistic effect, among which the synergistic effect was best when the ratio was 1:7; the co-toxicity coefficient of trifluanid and pymetrozine in the ratio of 1:50, 1:60, 1:70 and 1:80 to white-backed planthopper was greater than 120, showing a synergistic effect, and the co-toxicity coefficient was as high as 200.00 at 1:60, showing the best synergistic effect.

[0062] Example 2

[0063] Preparation of compound composition preparation.

[0064] 1.32% trifluanid·nitenpyram water dispersible granules (1:7)

[0065] The components are as follows (each component is calculated by weight percentage):

[0066] The original drug of trifluanid (100% off) is 4%, the original drug of nitenpyram (100% off) is 28%, the dispersant of sodium alkylnaphthalene sulfonate formaldehyde condensate is 3%, the dispersant of polycarboxylate is 3%, the wetting agent of sodium dodecyl sulfate is 3%, the disintegrant of ammonium sulfate is 3%, the filler of white carbon is 20%, and the filler of kaolin is supplemented to 100%. The preparation method is as follows: each active ingredient, dispersant, wetting agent, disintegrant and filler are fully mixed in proportion, crushed to a particle size of 5 μm by a jet mill to obtain a mother powder, the mother powder is fully mixed with an appropriate amount of water, granulated, dried and sieved, and a 32% water-dispersible granule of trifluanid and nitenpyram (1:7) is obtained.

[0067] 2.61% trifluanidazole·pymetrozine water dispersible granules (1:60)

[0068] The components are as follows (each component is calculated by weight percentage):

[0069] 1% of trifluanid original drug (100% off), 60% of pymetrozine original drug (100% off), 5% of sodium alkylnaphthalene sulfonate formaldehyde condensate dispersant, 5% of sodium lignin sulfonate dispersant, 3% of sodium alkylnaphthalene sulfonate wetting agent, 5% of ammonium sulfate disintegrant, and 100% of white carbon filler. The preparation method is as follows: each active ingredient, dispersant, wetting agent, disintegrant, and filler are fully mixed in proportion, crushed to a particle size of 5 μm by a jet mill to obtain a mother powder, and the mother powder is fully mixed with an appropriate amount of water, granulated, dried, and sieved to obtain 61% trifluanid·pymetrozine water dispersible granules (1:60).

[0070] Example 3

[0071] Field efficacy test of triflupromide compound composition for controlling white-backed planthopper.

[0072] The experiment has 6 treatments:

[0073] Treatment group 1: application of 10% trifluanid suspension (purchased from Corteva Agricultural Technology Co., Ltd.);

[0074] Treatment group 2: application of 20% nitenpyram soluble concentrate (purchased from Shandong Xinxing Pesticide Co., Ltd.);

[0075] Treatment group 3: application of 50% pymetrozine water dispersible granules (purchased from Jiangsu Yancheng Limin Agrochemical Co., Ltd.);

[0076] Treatment group 4: 32% trifluanid·nitenpyram water dispersible granules (1:7) were applied;

[0077] Treatment group 5: 61% trifluanid·pymetrozine water dispersible granules (1:60) were applied;

[0078] Treatment group 6: sprayed with clean water was used as a control (CK).

[0079] The specific dosages of the above 6 treatments are shown in Table 4.

[0080] The experimental field was set up in Wangzhuang, Wudun Town, Qingjiangpu District, Huai'an City, Jiangsu Province. Each treatment was repeated 4 times and arranged in random blocks. Each plot was 30m2. 2 A JACTO PJB-16 electric sprayer was used to evenly spray the stems and leaves of rice plants, with a water consumption of 675 L / hm2. 2 .

[0081] Before applying the pesticide, investigate the insect population base, and investigate the number of rice planthoppers remaining alive once at 3d, 7d, and 10d after applying the pesticide. Sample 10 points in each plot according to the parallel jump method, place the water-soaked white enamel plate obliquely at the base of the rice bush, shake the rice bush three times by hand, and count the total number of rice planthoppers that fall on the enamel plate. Investigate 2 rice bushes at each point, that is, investigate the number of planthoppers on 20 rice bushes in each plot. Calculate the control effect based on the reduction rate of rice planthoppers in the pesticide-treated area and the blank control area. The calculation formulas for the reduction rate of insect population and control effect are shown in Formula V and Formula VI:

[0082]

[0083] The test results are shown in Table 4.

[0084] Table 4 Field control effect of triflubenzuron compound composition on white-backed planthopper

[0085]

[0086] Note: The data in the table with different letters after them are significantly different (P<0.05) as tested by Duncan's new multiple range method.

[0087] As can be seen from Table 4, when trifluanid and nitenpyram were mixed at a ratio of 1:7, the control effects after 3d, 7d and 14d were 86.94%, 99.92% and 99.85%, respectively. When trifluanid and pymetrozine were mixed at a ratio of 1:60, the control effects after 3d, 7d and 14d were 91.45%, 99.61% and 99.74%, respectively. Under the condition that the dosage was much lower than that of a single agent, the two compound compositions showed good rapid and persistent effects in controlling white-backed planthoppers, with no significant difference from the single agent of trifluanid and nitenpyram, and significantly better than the control effect of pymetrozine alone. Field observations showed that the above-mentioned pesticide treatments were safe for the test crop rice, and no symptoms of pesticide damage (such as dwarfing, chlorosis and deformity, etc.) occurred.

[0088] In summary, when trifluanid and nitenpyram or trifluanid and pymetrozine are compounded in the pesticide composition of the present invention, a synergistic effect is exhibited under certain ratio conditions, and the control effect on white-backed planthoppers can be improved. Based on this, the application dosage of chemical agents can be reduced, and the problems of pesticide residues and environmental pollution caused by high-dose application can be avoided to a certain extent.

[0089] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creative work, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A pesticide composition for controlling white-backed planthopper containing trifluanid, characterized in that: The invention comprises an effective active ingredient A and an active ingredient B, wherein the active ingredient A is trifluanid and the active ingredient B is pymetrozine or nitenpyram.

2. The pesticide composition according to claim 1, characterized in that: When the active ingredient B is nitenpyram, the mass ratio of trifluanid to nitenpyram is 1:(4-8).

3. The pesticide composition according to claim 1, characterized in that: When the active ingredient B is pymetrozine, the mass ratio of trifluanid to pymetrozine is 1:(30-100).

4. A pesticide preparation, characterized in that: The pesticide preparation comprises the pesticide composition according to any one of claims 1 to 3 and pharmaceutically acceptable excipients.

5. The pesticide preparation according to claim 4, wherein the dosage form of the pesticide preparation comprises a suspension concentrate, a dispersible oil suspension concentrate, a suspension seed coating agent, a wettable powder or a water dispersible granule.

6. A water dispersible granule containing trifluanid for controlling white-backed planthopper, characterized in that: The raw materials for preparing the water-dispersible granules include the pesticide composition according to any one of claims 1 to 3 and auxiliary materials; the auxiliary materials include one or more of alkyl naphthalene sulfonate sodium formaldehyde condensate dispersant, polycarboxylate dispersant, sodium dodecyl sulfate wetting agent, sodium lignin sulfonate dispersant, alkyl naphthalene sulfonate sodium wetting agent, ammonium sulfate disintegrant, white carbon black filler and kaolin filler.

7. The method for preparing the water dispersible granules according to claim 6, comprising the following steps: The pesticide composition for controlling white-backed planthoppers containing triflubenzuron is mixed with auxiliary materials to obtain a mixed raw material; the obtained mixed raw material is crushed to obtain a mother powder; the mother powder is mixed with water, granulated, dried and sieved to obtain a water-dispersible granule.

8. The method for preparing the pesticide formulation according to claim 7, characterized in that: The pulverizing operation includes pulverizing using a jet mill.

9. The method for preparing the pesticide formulation according to claim 7, characterized in that: The particle size of the master powder is 5 to 10 μm.

10. Use of the triflufenazine-containing pesticide composition for controlling white-backed planthoppers according to any one of claims 1 to 3, or the pesticide preparation according to claim 4 or 5, or the water-dispersible granules according to claim 6, or the water-dispersible granules obtained by the preparation method of the water-dispersible granules according to claims 7 to 9 in controlling white-backed planthoppers.

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