Medicament for treating infectious bursal disease as well as preparation method and application thereof

Abstract

The invention relates to a traditional Chinese medicine preparation for treating infectious bursal disease as well as a preparation method and application of the traditional Chinese medicine preparation. The medicament is prepared from the following raw material components: 45 to 50 parts of radix astragali, 30 to 45 parts of radix rehmanniae, 25 to 30 parts of radix isatidis, 25 to 30 parts of folium isatidis, 10 to 45 parts of gypsum, 10 to 20 parts of honeysuckle flower, 10 to 30 parts of fructus forsythiae, 10 to 20 parts of herba taraxaci, 15 to 20 parts of rhizoma atractylodis macrocephalae, 15 to 30 parts of radix paeoniae rubra, 15 parts of bulbus lilii, 15 parts of rhizoma anemarrhenae and 10 to 30 parts of liquorice root. The medicament is prepared by extraction and concentration processes, and can effectively treat the chicken infectious bursal disease caused by the traditional infectious bursal disease virus (IBDV) and the novel variable infectious bursal disease virus (nvvIBDV). The medicine can significantly improve the cure rate of traditional and variant strains, and especially has significant advantages in the treatment of variant strains.

Description

Technical Field

[0001] The invention belongs to the technical field of disease treatment, and in particular relates to a medicament for treating infectious bursal disease, a preparation method and application thereof. Background Art

[0002] Infectious Bursal Disease (IBD) is an acute, highly contagious poultry disease caused by infectious bursal disease virus (IBDV), which mainly affects the bursa of Fabricius and the immune system of chickens, leading to immunosuppression and high mortality. The disease is widely prevalent in the global poultry industry, causing huge economic losses to the breeding industry. Especially in chicks, the incidence and mortality of IBD are high, which seriously affects the growth and production performance of chickens. In recent years, due to the continuous mutation of the virus, especially the emergence of new variant infectious bursal disease virus (nvvIBDV), the complexity of the disease and the difficulty of treatment have increased.

[0003] At present, the drugs used in the market for the treatment of infectious bursal disease in chickens are mainly chemically synthesized drugs and vaccines. However, the effects of these treatments are often unsatisfactory, especially for infectious bursal disease caused by mutant viruses, the efficacy of existing drugs is obviously insufficient. The antigenicity and virulence of mutant viruses have changed, which greatly reduces the protective effect of traditional vaccines, and the direct inhibitory effect of chemical drugs on viruses is limited, which easily leads to drug residues and drug resistance problems. Therefore, there is an urgent need to find new treatments to effectively deal with infectious bursal disease caused by these mutant strains.

[0004] Traditional Chinese medicine has a long history and rich experience in treating poultry diseases. There are many advantages to using traditional Chinese medicine to treat infectious bursal disease in chickens. First, the synergistic effect of multiple components of traditional Chinese medicine can regulate the body's immune function as a whole and improve disease resistance. Secondly, traditional Chinese medicine has low toxicity and side effects, is not easy to develop drug resistance, and has good safety and eco-friendliness. In addition, many traditional Chinese medicines have antiviral, anti-inflammatory and immunomodulatory effects, and have potential therapeutic effects on infectious bursal disease caused by new mutant viruses. Therefore, the development of formulas based on traditional Chinese medicine can provide an effective and sustainable solution for the prevention and treatment of infectious bursal disease in chickens. Summary of the invention

[0005] The purpose of the present invention is to provide a medicament for treating infectious bursal disease and a preparation method and application thereof, thereby solving the problem of treating infectious bursal disease caused by a new variant virus.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a medicament for treating infectious bursal disease, wherein the raw materials for preparing the medicament are composed of the following components:

[0008] 45-50 parts of Astragalus, 30-45 parts of Rehmannia, 25-30 parts of Isatis, 25-30 parts of Folium Isatidis, 10-45 parts of Gypsum, 10-20 parts of Honeysuckle, 10-30 parts of Forsythia, 10-20 parts of Taraxacum, 15-20 parts of Atractylodes, 15-30 parts of Red Peony Root, 15 parts of Lilium, 15 parts of Anemarrhena, and 10-30 parts of Liquorice.

[0009] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

[0010] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by the novel variant infectious bursal disease virus nvvIBDV.

[0011] Furthermore, the medicament is prepared by the following preparation method:

[0012] (1) Weigh different components according to the composition of the raw materials and add them into the extraction device;

[0013] (2) Add 10 times the amount of distilled water, heat to 100°C, and boil for 1.5 hours;

[0014] (3) Filter and collect the filtrate 1 and the residue, add 10 times the amount of distilled water to the residue, heat to 90°C, and boil for 1.5 hours;

[0015] (4) collecting filtrate 2 by filtration, and combining filtrate 1 and filtrate 2 to obtain filtrate 3;

[0016] (5) The filtrate 3 is concentrated to 1 / 10 of its original volume by rotary evaporation to obtain the pharmaceutical agent.

[0017] Furthermore, the raw materials for preparing the medicine are composed of the following components: 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Isatis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Honeysuckle, 20 parts of Forsythia, 20 parts of Taraxacum, 15 parts of Atractylodes, 15 parts of Red Peony, 15 parts of Lilium, 15 parts of Anemarrhena, and 10 parts of Licorice.

[0018] In a second aspect, the present invention provides a method for preparing a medicament for treating infectious bursal disease, the preparation method comprising the following steps:

[0019] (1) Add 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae into an extraction device;

[0020] (2) Add 10 times the amount of distilled water, heat to 100°C, and boil for 1.5 hours;

[0021] (3) Filter and collect the filtrate 1 and the residue, add 10 times the amount of distilled water to the residue, heat to 90°C, and boil for 1.5 hours;

[0022] (4) collecting filtrate 2 by filtration, and combining filtrate 1 and filtrate 2 to obtain filtrate 3;

[0023] (5) The filtrate 3 is concentrated to 1 / 10 of its original volume by rotary evaporation to obtain the pharmaceutical agent.

[0024] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

[0025] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by the novel variant infectious bursal disease virus nvvIBDV.

[0026] In a third aspect, the present invention provides an application of a traditional Chinese medicine composition in the preparation of a medicine for treating infectious bursal disease, wherein the traditional Chinese medicine composition is composed of 45-50 parts of astragalus, 30-45 parts of rehmannia, 25-30 parts of isatis root, 25-30 parts of isatis indigotica, 10-45 parts of gypsum, 10-20 parts of honeysuckle, 10-30 parts of forsythia, 10-20 parts of dandelion, 15-20 parts of atractylodes, 15-30 parts of red peony root, 15 parts of lily, 15 parts of anemarrhena, and 10-30 parts of licorice.

[0027] Further, the composition of the Chinese medicine composition is 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae;

[0028] The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

[0029] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by the novel variant infectious bursal disease virus nvvIBDV.

[0030] In a fourth aspect, the present invention provides an application of a traditional Chinese medicine composition in the preparation of a medicine for inhibiting infectious bursal disease virus, characterized in that the traditional Chinese medicine composition is composed of 45-50 parts of astragalus, 30-45 parts of rehmannia, 25-30 parts of isatis root, 25-30 parts of isatis indigotica, 10-45 parts of gypsum, 10-20 parts of honeysuckle, 10-30 parts of forsythia, 10-20 parts of dandelion, 15-20 parts of atractylodes, 15-30 parts of red peony root, 15 parts of lily, 15 parts of anemarrhena, and 10-30 parts of licorice.

[0031] Further, the composition of the Chinese medicine composition is 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae;

[0032] The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

[0033] Furthermore, the infectious bursal disease is infectious bursal disease in chickens caused by the novel variant infectious bursal disease virus nvvIBDV.

[0034] The beneficial effects of the present invention are:

[0035] In the medicament for treating infectious bursal disease provided by the present invention, each component has obvious synergistic effect. In particular, the combination of key components such as astragalus, honeysuckle, forsythia, isatis root, and isatis leaf can mutually enhance the immune regulation, antiviral, heat-clearing and detoxifying functions of the medicine. The combination of such components can not only effectively improve the body's immunity, but also inhibit virus replication and promote virus clearance, so that the medicine shows significant therapeutic effects when dealing with traditional strains and variant strains.

[0036] The formula in Example 1, through precise ingredient ratios and optimized combinations, exhibits stronger antiviral ability when facing the new variant infectious bursal disease virus (nvvIBDV). The experimental results in Tables 1 and 2 show that Example 1 has better inhibitory effects on variant strains than other examples (2-6) and comparative examples (1-10), especially in reducing the amount of virus excretion and improving the cure rate. Example 1 shows significant advantages. This shows that the ingredient ratio in Example 1 is particularly suitable for dealing with more toxic and drug-resistant variant viruses, producing unexpected technical effects. DETAILED DESCRIPTION

[0037] Example 1

[0038] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0039] The medicine includes the following components:

[0040] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae;

[0041] The preparation method of the drug is as follows:

[0042] (1) Weigh different components according to the formula and add them into the extraction tank;

[0043] (2) Add 10 times the amount of distilled water, heat to 100°C, and boil for 1.5 hours;

[0044] (3) Filter and collect the filtrate 1 and the residue, add 10 times the amount of distilled water to the residue, heat to 90°C, and boil for 1.5 hours;

[0045] (4) collecting filtrate 2 by filtration, and combining filtrate 1 and filtrate 2 to obtain filtrate 3;

[0046] (5) The filtrate 3 is concentrated to 1 / 10 of its original volume by rotary evaporation to obtain a traditional Chinese medicine for treating variant infectious bursal disease.

[0047] Example 2

[0048] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0049] The medicine includes the following components:

[0050] 50 parts of Astragalus, 25 parts of Rehmannia, 25 parts of Radix Isatidis, 25 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 20 parts of Atractylodes macrocephalae, 15 parts of Paeonia lactiflora, 20 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0051] The preparation method is the same as that of Example 1

[0052] Example 3

[0053] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0054] The medicine includes the following components:

[0055] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 10 parts of Flos Lonicerae, 10 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 20 parts of Atractylodes, 15 parts of Radix Paeoniae Rubra, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 25 parts of Radix Glycyrrhizae.

[0056] The preparation method is the same as that of Example 1

[0057] Example 4

[0058] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0059] The medicine includes the following components:

[0060] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 10 parts of Flos Lonicerae, 30 parts of Fructus Forsythiae, 10 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 25 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0061] The preparation method is the same as that of Example 1

[0062] Example 5

[0063] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0064] The medicine includes the following components:

[0065] 45 parts of Astragalus, 45 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 10 parts of Gypsum, 10 parts of Flos Lonicerae, 30 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 30 parts of Radix Glycyrrhizae.

[0066] The preparation method is the same as that of Example 1

[0067] Example 6

[0068] This embodiment provides a Chinese medicine for treating variant infectious bursal disease

[0069] The medicine includes the following components:

[0070] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 30 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 30 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0071] The preparation method is the same as Example 1.

[0072] Comparative Example 1

[0073] This comparative example provides a Chinese medicinal preparation lacking Astragalus membranaceus

[0074] The Chinese medicine comprises the following components:

[0075] 30 parts of Rehmannia root, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0076] The preparation method is the same as Example 1.

[0077] Comparative Example 2

[0078] This comparative example provides a Chinese medicinal preparation lacking Folium Isatidis

[0079] The Chinese medicine comprises the following components:

[0080] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Radix Paeoniae Rubra, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0081] The preparation method is the same as Example 1.

[0082] Comparative Example 3

[0083] This comparative example provides a Chinese medicinal preparation lacking honeysuckle

[0084] The Chinese medicine comprises the following components:

[0085] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0086] The preparation method is the same as Example 1.

[0087] Comparative Example 4

[0088] This comparative example provides a Chinese medicinal preparation lacking Forsythia suspensa

[0089] The Chinese medicine comprises the following components:

[0090] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Radix Paeoniae Rubra, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0091] The preparation method is the same as Example 1.

[0092] Comparative Example 5

[0093] This comparative example provides a Chinese medicinal preparation lacking Radix Isatidis

[0094] The Chinese medicine comprises the following components:

[0095] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0096] The preparation method is the same as Example 1.

[0097] Comparative Example 6

[0098] This example provides a Chinese medicine preparation using Codonopsis pilosula instead of Astragalus membranaceus

[0099] The Chinese medicine comprises the following components:

[0100] 45 parts of Codonopsis pilosula, 30 parts of Rehmannia root, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0101] The preparation method is the same as Example 1.

[0102] Comparative Example 7

[0103] This example provides a Chinese medicine preparation using purslane instead of isatis indigotica

[0104] The Chinese medicine comprises the following components:

[0105] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Portulaca Oleracea, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0106] The preparation method is the same as Example 1.

[0107] Comparative Example 8

[0108] This example provides a Chinese medicine preparation using chrysanthemum to replace honeysuckle

[0109] The Chinese medicine comprises the following components:

[0110] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Chrysanthemi, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Radix Paeoniae Rubra, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0111] The preparation method is the same as Example 1.

[0112] Comparative Example 9

[0113] This example provides a Chinese medicine preparation using mulberry leaves instead of forsythia suspensa

[0114] The Chinese medicine comprises the following components:

[0115] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Folium Mori, 20 parts of Herba Taraxaci, 15 parts of Atractylodes macrocephalae, 15 parts of Radix Paeoniae Rubra, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0116] The preparation method is the same as Example 1.

[0117] Comparative Example 10

[0118] This example provides a Chinese medicine preparation using Perilla to replace Radix Isatidis

[0119] The Chinese medicine comprises the following components:

[0120] 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Perilla, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae.

[0121] The preparation method is the same as Example 1.

[0122] Experimental test: Difference in cure rate of infectious bursal disease in chickens without medication

[0123] Experimental steps:

[0124] Experimental Materials:

[0125] Strains: standard infectious bursal disease virus (IBDV), novel variant infectious bursal disease virus (nvvIBDV);

[0126] Experimental animals: 350 21-day-old SPF chicks were purchased from Beijing Boehringer Ingelheim Weitong Biotechnology Co., Ltd.;

[0127] Experimental animal grouping: 350 21-day-old SPF chickens were randomly divided into 35 groups, namely: groups 1-6 of examples of traditional IBDV virus challenge, groups 1-10 of comparative examples; groups 1-6 of examples of variant strains, groups 1-10 of comparative examples,

[0128] At the same time, a traditional IBDV infection control group, a variant strain control group and a healthy control group were set up, with 10 mice in each group. The drug administration group, infection control group and healthy control group were kept in different isolators to prevent cross infection.

[0129] Challenge and drug administration: Before drug administration, SPF chickens were inoculated with 0.3 mL of IBDV and nvvIBDV (containing 104-EID50 / 0.3 ml) intranasally, and the chickens were given the drug orally by gavage 24 hours after virus inoculation for 5 consecutive days, once a day.

[0130] Cure rate and survival rate determination: The number of deaths was recorded 2-5 days after IBDV and variant strains were inoculated. The number of infected chickens that were wilted and depressed was measured before administration, and the chickens that recovered and recovered their spirits and eating 5 days after administration were recorded as recovered chickens. Cure rate (%) = number of recovered chickens / number of diseased chickens × 100%.

[0131] Table 1 Differences in cure rates after treatment with different drugs

[0132]

[0133]

[0134]

[0135] It can be seen from Table 1 that the drug prepared in Example 1 has the best therapeutic effect on infectious bursal disease in chickens, and achieves a 100% cure rate for infectious bursal disease in chickens caused by traditional strains and mutant strains.

[0136] Comparison between Example 1 and Example 2:

[0137] Astragalus: 45 parts in Example 1 and 50 parts in Example 2. Although the amount of Astragalus increases, the change in its proportion affects the coordination of other ingredients;

[0138] Rehmannia root: 30 parts in Example 1 and 25 parts in Example 2. The higher proportion of Rehmannia root in Example 1 may enhance the yin nourishing effect and help restore the body's immunity.

[0139] Compared with Example 3, Example 1:

[0140] Honeysuckle and Forsythia: The honeysuckle and Forsythia in Example 3 are reduced to 10 parts and 10 parts respectively, which may significantly reduce the antiviral ability and affect the overall effect.

[0141] Licorice: 10 parts in Example 1 and 25 parts in Example 3. Although the addition of licorice helps to balance the medicinal properties, an excessive amount may inhibit the effects of other ingredients.

[0142] Compared with Example 4, Example 1:

[0143] Forsythia suspensa: In Example 4, the amount of Forsythia suspensa was increased to 30 parts, which may lead to an imbalance in the heat-clearing and detoxifying effects of the prescription and interfere with the overall synergy of the drugs.

[0144] Gypsum: The amount of gypsum in Example 1 is 45 parts, while that in Example 4 is reduced to 30 parts, which may reduce the effect of clearing heat toxins and affect the resistance to mutant strains.

[0145] Compared with Example 5, Example 1:

[0146] Gypsum: The amount of gypsum in Example 5 was reduced to 10 parts, and the heat-clearing effect was greatly weakened, which may lead to insufficient inhibition of the virus.

[0147] Rehmannia root: In Example 5, the amount of Rehmannia root was increased to 45 parts. Although the tonic effect was enhanced, the resistance to viruses was relatively weakened.

[0148] Comparison between Example 1 and Example 6

[0149] Red peony root: In Example 6, the amount of red peony root increased to 30 parts, which may enhance the blood circulation effect, but may not necessarily increase the antiviral effect.

[0150] Gypsum: The amount of gypsum in Example 6 is 30 parts, which has lower heat-clearing efficiency than 45 parts in Example 1, thus possibly leading to poor effect on mutant strains.

[0151] In general, Example 1 shows better overall therapeutic effect due to its reasonable balance in clearing heat, detoxifying and replenishing qi, especially when targeting mutant strains, which produces unexpected technical effects.

[0152] Compared with Example 1, the therapeutic effects of Examples 1-5 on infectious bursal disease in chickens caused by traditional strains and mutant strains are greatly reduced, indicating that the missing components of Examples 1 to 5 have a significant impact on the overall effect of the agents, especially the lack of the key immune-enhancing and antiviral ingredients Astragalus, Honeysuckle, Forsythia and Isatis root, which makes these agents significantly less effective than Example 1 when dealing with mutant strains.

[0153] Compared with Example 1, Comparative Example 6 (replacing Astragalus with Codonopsis pilosula), Comparative Example 7 (replacing Folium Isatidis with Portulaca oleracea), Comparative Example 8 (replacing Flos Lonicerae with Flos Chrysanthemi), Comparative Example 9 (replacing Fructus Forsythiae with Folium Mulberrye), Comparative Example 10 (replacing Radix Isatidis with Fructus Perillae), the overall effects of the medicaments of Comparative Examples 6 to 10 are significantly reduced compared with Example 1 due to the lack or replacement of components. In particular, the key roles of components such as Astragalus, Flos Lonicerae, Fructus Forsythiae and Radix Isatidis in immunomodulation, antiviral and heat-clearing and detoxification are weakened, resulting in the therapeutic effect on the variant strain being far less than that of Example 1. This difference in components directly affects the overall efficacy of the medicament.

[0154] In summary, Example 1, due to its unique combination of ingredients and optimized ratio, the synergistic effect between the ingredients significantly demonstrates unexpected technical effects, has significant advantages in treating infectious bursal disease caused by mutant strains, and can effectively improve the overall efficacy of the drug.

[0155] Experimental test

[0156] After the observation, necropsy was performed, with 10 chickens in each group. The bursa of Fabricius was harvested and the weight of the bursa and the chicken body weight were weighed. The bursal tissue was ground and RNA was extracted. qRT-PCR quantification was performed according to the kit steps, and organ lesions were observed.

[0157] Table 2

[0158]

[0159]

[0160] It can be seen from the results in Table 2 that, consistent with the results obtained in Table 1, the toxin excretion amounts of the traditional strains and variant strains in Example 1 are significantly lower than those in other examples and comparative examples, indicating that the drug prepared in Example 1 has a significant inhibitory effect on both the traditional IBDV strains and the variant nvvIBDV strains. In comparison with Examples 2-6, it can be found that although Examples 2-6 also have a relatively good inhibitory effect on the traditional strains, the inhibitory effect on the variant strains is relatively general.

[0161] The above results show that in the drug formula of Example 1, the combination of Astragalus, Honeysuckle and Forsythia enhances the body's immunity, inhibits viral replication and accelerates viral clearance. In addition, ingredients such as Radix Isatidis and Folium Isatidis significantly reduce viral emissions by directly resisting viruses and removing toxins. The synergistic effect of these ingredients enables Example 1 to show excellent therapeutic effects on both traditional strains and variant strains.

[0162] Examples 2-6 differ from Example 1 in the composition ratio, for example, the amount of Astragalus, Rehmannia, Radix Isatidis, Gypsum, etc. is adjusted, or the content of some ingredients (such as Honeysuckle and Forsythia) is reduced. These adjustments lead to the weakening of the immunomodulatory and antiviral abilities of the drugs when fighting against viruses, especially when facing the mutant strains of the present invention.

[0163] Therefore, the formula of Example 1 is particularly suitable for combating mutant strains with stronger infectivity and the ability to evade the host immune system through precise ingredient ratios and optimized combinations, and the ingredient combination of Example 1 can significantly enhance the host's immune response and directly interfere with the virus replication process, showing a higher inhibitory effect.

[0164] Compared with Example 1, after the key formula ingredients of Comparative Examples 1-10 are missing or replaced, the immunomodulatory ability and antiviral effect of the drug are significantly reduced, especially when facing more challenging mutant strains, the therapeutic effect is even more unsatisfactory. This shows that the combination of ingredients in Example 1 has a significant synergistic effect, and the components support each other in enhancing the body's immunity, clearing away heat and detoxifying, and inhibiting viral replication, forming an effective overall therapeutic effect. Once certain ingredients are removed or replaced, this synergistic effect will be destroyed, resulting in a significant decrease in the effect of the drug in treating traditional strains and mutant strains.

[0165] Therefore, the success of Example 1 lies not only in the effect of each component alone, but also in the precise ratio and mutual synergy between them. The scientific nature of this combination makes the drug show unexpectedly excellent efficacy in enhancing immune function, resisting viruses and controlling mutant strains, but once this balance is broken, the overall effect of the drug is significantly reduced.

Claims

1. A medicament for treating infectious bursal disease, characterized in that: The raw materials for preparing the medicament are composed of the following components: 45-50 parts of Astragalus, 30-45 parts of Rehmannia, 25-30 parts of Isatis, 25-30 parts of Folium Isatidis, 10-45 parts of Gypsum, 10-20 parts of Honeysuckle, 10-30 parts of Forsythia, 10-20 parts of Taraxacum, 15-20 parts of Atractylodes, 15-30 parts of Red Peony Root, 15 parts of Lilium, 15 parts of Anemarrhena, and 10-30 parts of Liquorice.

2. The drug according to claim 1, characterized in that The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

3. The medicament according to claim 2, characterized in that The medicament is prepared by the following preparation method: (1) Weigh different components according to the composition of the raw materials and add them into the extraction device; (2) Add 10 times the amount of distilled water, heat to 100°C, and boil for 1.5 hours; (3) Filter and collect the filtrate 1 and the residue, add 10 times the amount of distilled water to the residue, heat to 90°C, and boil for 1.5 hours; (4) collecting filtrate 2 by filtration, and combining filtrate 1 and filtrate 2 to obtain filtrate 3; (5) The filtrate 3 is concentrated to 1 / 10 of its original volume by rotary evaporation to obtain the pharmaceutical agent.

4. The medicament according to claim 3, characterized in that The raw materials for preparing the medicine are composed of the following components: 45 parts of astragalus, 30 parts of rehmannia, 30 parts of isatis root, 30 parts of isatis indigotica, 45 parts of gypsum, 20 parts of honeysuckle, 20 parts of forsythia, 20 parts of dandelion, 15 parts of atractylodes, 15 parts of red peony root, 15 parts of lily, 15 parts of anemarrhena, and 10 parts of liquorice.

5. A method for preparing a medicament for treating infectious bursal disease, characterized in that: The preparation method comprises the following steps: (1) Add 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Radix Isatidis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Flos Lonicerae, 20 parts of Fructus Forsythiae, 20 parts of Herba Taraxaci, 15 parts of Atractylodes, 15 parts of Paeonia lactiflora, 15 parts of Lilium, 15 parts of Rhizoma Anemarrhenae, and 10 parts of Radix Glycyrrhizae into an extraction device; (2) Add 10 times the amount of distilled water, heat to 100°C, and boil for 1.5 hours; (3) Filter and collect the filtrate 1 and the residue, add 10 times the amount of distilled water to the residue, heat to 90°C, and boil for 1.5 hours; (4) collecting filtrate 2 by filtration, and combining filtrate 1 and filtrate 2 to obtain filtrate 3; (5) The filtrate 3 is concentrated to 1 / 10 of its original volume by rotary evaporation to obtain the pharmaceutical agent.

6. The preparation method according to claim 5, characterized in that: The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

7. Use of a Chinese medicine composition in the preparation of a drug for treating infectious bursal disease, characterized in that: The Chinese medicine composition comprises: 45-50 parts of astragalus, 30-45 parts of rehmannia, 25-30 parts of isatis root, 25-30 parts of isatis indigotica, 10-45 parts of gypsum, 10-20 parts of honeysuckle, 10-30 parts of forsythia, 10-20 parts of dandelion, 15-20 parts of atractylodes, 15-30 parts of red peony root, 15 parts of lily, 15 parts of anemarrhena, and 10-30 parts of liquorice.

8. The use according to claim 7, characterized in that: The Chinese medicine composition is composed of 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Isatis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Honeysuckle, 20 parts of Forsythia, 20 parts of Taraxacum, 15 parts of Atractylodes, 15 parts of Red Peony, 15 parts of Lilium, 15 parts of Anemarrhena, and 10 parts of Liquorice; The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

9. Use of a Chinese medicine composition in the preparation of a drug for inhibiting infectious bursal disease virus, characterized in that: The Chinese medicine composition comprises: 45-50 parts of astragalus, 30-45 parts of rehmannia, 25-30 parts of isatis root, 25-30 parts of isatis indigotica, 10-45 parts of gypsum, 10-20 parts of honeysuckle, 10-30 parts of forsythia, 10-20 parts of dandelion, 15-20 parts of atractylodes, 15-30 parts of red peony root, 15 parts of lily, 15 parts of anemarrhena, and 10-30 parts of liquorice.

10. The use according to claim 9, characterized in that: The Chinese medicine composition is composed of 45 parts of Astragalus, 30 parts of Rehmannia, 30 parts of Isatis, 30 parts of Folium Isatidis, 45 parts of Gypsum, 20 parts of Honeysuckle, 20 parts of Forsythia, 20 parts of Taraxacum, 15 parts of Atractylodes, 15 parts of Red Peony, 15 parts of Lilium, 15 parts of Anemarrhena, and 10 parts of Liquorice; The infectious bursal disease is chicken infectious bursal disease caused by traditional infectious bursal disease virus IBDV and novel variant infectious bursal disease virus nvvIBDV.

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