Application of ZmNRL1 gene and its encoded protein in improving nitrogen use efficiency of plants

By mutating and overexpressing the maize ZmNRL1 gene, constructing a recombinant vector and transforming maize and Arabidopsis, the problem of low nitrogen utilization efficiency in maize was solved, nitrogen utilization efficiency and tolerance to low nitrogen stress were improved, biomass and nitrate nitrogen content were enhanced, and genetic resources were provided to support maize yield increases and environmental protection.

CN119431529BActive Publication Date: 2025-09-30SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202410241848.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-03-04
Publication Date
2025-09-30
Estimated Expiration
2044-03-04

AI Technical Summary

Technical Problem

In the existing technology, the nitrogen utilization efficiency of corn is low, resulting in the dependence of corn yield increase on large amounts of nitrogen fertilizer input, and excessive use of nitrogen fertilizer leads to environmental pollution. There is a lack of effective molecular mechanism research and genetic improvement methods.

Method used

By mutating and overexpressing the maize ZmNRL1 gene, constructing a recombinant vector and transforming maize and Arabidopsis, the plants' nitrogen utilization efficiency and tolerance to low nitrogen stress were improved, and the nitrate reductase activity and nitrate nitrogen content were enhanced.

Benefits of technology

It significantly improved the nitrogen utilization efficiency of corn and Arabidopsis, enhanced tolerance to low nitrogen stress, increased biomass and nitrate nitrogen content, and provided genetic resources to support corn yield increase and environmental protection.

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Abstract

The present invention discloses the application of the ZmNRL1 gene and its encoded protein in improving the nitrogen utilization efficiency of plants. The nucleotide sequence of the ZmNRL1 gene is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO.2. The present invention finds that the corn ZmNRL1 gene can effectively regulate the nitrogen utilization efficiency of plants. After mutating ZmNRL1, corn seedlings can exhibit a nitrogen starvation-sensitive phenotype, while overexpressing ZmNRL1 can improve the ability of corn seedlings and Arabidopsis plants to tolerate low nitrogen stress, while increasing plant biomass, nitrate reductase activity and nitrate nitrogen content. The present invention provides genetic resources for cultivating new low-nitrogen-tolerant corn germplasm, which is of great significance for increasing corn yield and improving nitrogen utilization efficiency.
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